Digital Commons Network^™

Evaluation Of Gliovascular Functions Of Aqp4 Readthrough Isoforms, Shayna M Mueller, Kelli Mcfarland White, Stuart B Fass, Siyu Chen, Zhan Shi, Xia Ge, John A Engelbach, Seana H Gaines, Annie R Bice, Michael J Vasek, Joel R Garbow, Joseph P Culver, Zila Martinez-Lozada, Martine Cohen-Salmon, Joseph D Dougherty, Darshan Sapkota

2020-Current year OA Pubs

Aquaporin-4 (AQP4) is a water channel protein that links the astrocytic endfeet to the blood-brain barrier (BBB) and regulates water and potassium homeostasis in the brain, as well as the glymphatic clearance of waste products that would otherwise potentiate neurological diseases. Recently, translational readthrough was shown to generate a C-terminally extended variant of AQP4, known as AQP4x, which preferentially localizes around the BBB through interaction with the scaffolding protein α-syntrophin, and loss of AQP4x disrupts waste clearance from the brain. To investigate the function of AQP4x, we generated a novel AQP4 mouse line (AllX) to increase relative levels of the …

The Actin-Bundling Protein, Pls3, Is Part Of The Mechanoresponsive Machinery That Regulates Osteoblast Mineralization, Samantha M Chin, Carmela Unnold-Cofre, Teri Naismith, Silvia Jansen

2020-Current year OA Pubs

Plastin-3 (PLS3) is a calcium-sensitive actin-bundling protein that has recently been linked to the development of childhood-onset osteoporosis. Clinical data suggest that PLS3 mutations lead to a defect in osteoblast function, however the underlying mechanism remains elusive. To investigate the role of PLS3 in bone mineralization, we generated MC3T3-E1 preosteoblast cells that are stably depleted of PLS3. Analysis of osteogenic differentiation of control and PLS3 knockdown (PLS3 KD) cells showed that depletion of PLS3 does not alter the first stage of osteoblast mineralization in which a collagen matrix is deposited, but severely affects the subsequent mineralization of that matrix. During …

Whole Genome Sequence Analysis Of Apparent Treatment Resistant Hypertension Status In Participants From The Trans-Omics For Precision Medicine Program, Nicole D Armstrong, Dabeeru C Rao, Et Al.

2020-Current year OA Pubs

No abstract provided.

A Bone To Pick-Cellular And Molecular Mechanisms Of Bone Pain In Sickle Cell Disease, Jahnavi Gollamudi, Kristine A Karkoska, Oluwabukola T Gbotosho, Wei Zou, Hyacinth I Hyacinth, Steven L Teitelbaum

2020-Current year OA Pubs

The bone is one of the most commonly affected organs in sickle cell disease (SCD). Repeated ischemia, oxidative stress and inflammation within the bone is largely responsible for promoting bone pain. As more individuals with SCD survive into adulthood, they are likely to experience a synergistic impact of both aging and SCD on their bone health. As bone health deteriorates, bone pain will likely exacerbate. Recent mechanistic and observational studies emphasize an intricate relationship between bone remodeling and the peripheral nervous system. Under pathological conditions, abnormal bone remodeling plays a key role in the propagation of bone pain. In this …

Evaluating The Efficacy Of Purchased Antisense Oligonucleotides To Reduce Mouse And Human Tau In Vivo, Pranav Vemula, Kathleen M Schoch, Timothy M Miller

2020-Current year OA Pubs

Many preclinical and clinical studies support the use of antisense oligonucleotides (ASOs) as effective therapeutic strategies. However, acquiring ASOs for research purposes may be limited by partnerships with the pharmaceutical companies. Our lab previously developed an effective ASO strategy to lower human tau and reverse pathology in aged tauopathy model mice. Testing the efficacy of purchased tau lowering ASOs would provide support for these reagents as broad research tools. Purchased mouse and human tau lowering ASOs were infused or injected intracerebroventricularly into wildtype and tau transgenic mice. Following treatment, brain tissue evaluated for ASO distribution and levels of tau mRNA, …

Characterization Of Vernal Pools Across National Parks In The Great Lakes Region, Samantha R. Kurkowski

Dissertations, Master's Theses and Master's Reports

Vernal pools are small, ephemeral wetlands that become inundated each spring and provide many ecosystem services to the surrounding upland forests. They also provide critical habitat for amphibians and invertebrates, as their temporary nature keeps them free of fish and reduces predator populations. As part of a mapping project, we collected baseline field data on vernal pool characteristics throughout five Great Lakes National Parks: Pictured Rocks National Lakeshore, Sleeping Bear Dunes National Lakeshore, Apostle Islands National Lakeshore, Isle Royale National Park, and Voyagers National Parks. Our goals were to characterize and assess how vernal pools vary within and across the …

Eutrophication On Twin Lakes: Applying A Phosphorus Budget And Shoreline Surveys To Assess Current & Historical Lake Health, Tyler K. Lemahieu

Dissertations, Master's Theses and Master's Reports

The Twin Lakes system, a series of lakes including Lake Gerald and Lake Roland, was historically categorized as oligotrophic but is now mesotrophic having experienced cultural eutrophication. A mass balance phosphorus budget model was constructed for the Twin Lakes system in Houghton, Co. Michigan for the 2022 stratified season. Additional spatial insights were gleaned through a survey of shoreline water quality, which corroborated the model results. The lakes currently experience substantial depletion of hypolimnetic dissolved oxygen during late summer stratification. A budget reveals approximately 22% (6.6 kg) of the current 29.5 kg total phosphorus inputs to Twin Lakes (during the …

Structural Analysis And Slip Kinematics Of The Keweenaw Fault System Between Bête Grise Bay And Gratiot Lake, Keweenaw County, Michigan, Daniel J. Lizzadro-Mcpherson

Dissertations, Master's Theses and Master's Reports

The Keweenaw fault is perhaps the most significant geologic structure on the Keweenaw Peninsula, with an estimated 7-11 km of reverse slip juxtaposing volcanic strata of the ~1.1 Ga Portage Lake Volcanics above ~1.0 Ga Jacobsville Sandstone. The fault has been interpreted as a rift-bounding normal fault later inverted by compressional pulses of the Grenville Orogeny or, more recently, as part of a detached thrust fault system unrelated to an earlier normal fault. The fault is shown on published maps as a single continuous fault trace whose sinuosity implies multiple fault segments and complex slip dynamics. Mapping along Bête Grise …

Implementation And Optimization Of Multi-Resonance And Phase Control Of The Electrical Power Take-Off On A Wec Array For Improved Performance, Madelyn G. Veurink

Dissertations, Master's Theses and Master's Reports

Many governments around the world are pledging to reduce their consumption of fossil fuels as they look to curb the amount of green house gasses they release into the atmosphere. These green house gasses are what scientists blame for global warming and the recent increase in extreme weather events. Producing electricity is one of the largest producers of these gasses but utilizing renewable sources can greatly decrease the amount of green house gasses produced. Common forms of renewable energies are wind and solar and both of these green energies have reached a state of maturation where they are economically viable …

Neuroevolution And Machine Learning Research Applied To Connected Automated Vehicle And Powertrain Control, Frédéric F. Jacquelin

Dissertations, Master's Theses and Master's Reports

This dissertation focuses on advancing Predictive Energy Management (PrEM) functions applied to modern connected and automated vehicles (CAV) cohorts. PrEM aims to utilize connectivity and ADAS functions to adaptively minimize vehicle energy consumption in a wide array of operations, extending the original control designed around a reduced set of test cycle procedures to adapt to real-world stochastic operating conditions. This research document is built upon three journal publications covering two PrEM schemes; the global cohort and local vehicle optimization paths. Both optimal control solutions are generated using various Neuroevolution centric processes.

Chapter 1 discusses the methods and reasoning behind the …

Age And Chemistry Of Bell Creek Batholith, Elana G. Barth

Dissertations, Master's Theses and Master's Reports

The Southern Complex, located near Marquette, Michigan is in the southernmost portion of the Superior Province. This complex consists of granitoids and granitic gneiss that vary in composition. In twentieth century research, Hoffman (1987) and Tinkham (1997) concluded that the Southern Complex contained two units in which the granitoid was emplaced in the granitic gneiss. Recent research conducted by Petryk (2019) determined that the Southern Complex has a U-Pb zircon age of 2600 Ma. Dalle Fratte (2020) concluded that the granitic gneiss migmatite texture was the result of a felsic magma mixing with several generations of mafic intrusions that were …

Bioaccumulation Of Polychlorinated Biphenyl Compounds And Mercury In A Mining Impacted Aquatic Ecosystem, Michelle Bollini

Dissertations, Master's Theses and Master's Reports

The Keweenaw area continues to be influenced by the century of copper mining that ended nearly 50 years ago. This project is focused on Torch Lake, an aquatic ecosystem that has been heavily impacted by mining waste disposal. The watershed has been impaired by mine discharge and tailings, smelter and smokestack plumes, and poor waste disposal practices. The lake is listed as a Great Lakes Area of Concern with beneficial use impairments of restrictions on fish consumption and a degraded benthic community. Polychlorinated biphenyl compounds (PCBs) and methylmercury (MeHg) are persistent, bioaccumulative, and toxic substances (PBTs). These contaminants pose threats …

Multiscale Molecular Modeling Studies Of The Dynamics And Catalytic Mechanisms Of Iron(Ii)- And Zinc(Ii)-Dependent Metalloenzymes, Sodiq O. Waheed

Dissertations, Master's Theses and Master's Reports

Enzymes are biological systems that aid in specific biochemical reactions. They lower the reaction barrier, thus speeding up the reaction rate. A detailed knowledge of enzymes will not be achievable without computational modeling as it offers insight into atomistic details and catalytic species, which are crucial to designing enzyme-specific inhibitors and impossible to gain experimentally. This dissertation employs advanced multiscale computational approaches to study the dynamics and reaction mechanisms of non-heme Fe(II) and 2-oxoglutarate (2OG) dependent oxygenases, including AlkB, AlkBH2, TET2, and KDM4E, involved in DNA and histone demethylation. It also focuses on Zn(II) dependent matrix metalloproteinase-1 (MMP-1), which helps …

Information Use And Decision-Making For Evacuation At Fuego Volcano, Guatemala, Beth A. Bartel

Dissertations, Master's Theses and Master's Reports

For populations living with risk to rapid-onset environmental hazards, an effective early warning system (EWS) may be the most viable short- to mid-term solution for risk reduction. At Fuego volcano, Guatemala, more than 60,000 people distributed between more than 30 small communities live within the identified hazard zones for pyroclastic density currents (PDCS), highly lethal hot avalanches and surges of volcanic gases, rock, and ash. Despite ongoing risk reduction efforts by scientific and civil protection authorities, more than 400 people died during a paroxysmal eruption on 3 June 2018 when PDCs reached populated areas. A high-end resort, La Reunión, evacuated …

An Exploration Of Cloud Droplet Growth By Condensation And Collision-Coalescence In A Convection-Cloud Chamber, Jacob T. Kuntzleman

Dissertations, Master's Theses and Master's Reports

Atmospheric clouds are crucial to weather and climate, and the rate at which droplets collide and coalesce to form precipitation is one of the fundamental controlling processes. The convection-cloud chamber allows the interactions between aerosols and cloud droplets produced by condensation to be investigated within a turbulent environment. Studying the full range of microphysical conditions in atmospheric clouds is not possible, however, unless conditions for droplet growth by collision and coalescence are also achieved. In this study, we explore the conditions favorable to collision-coalescence growth in convection-cloud chambers, extending previous work on steady-state droplet size distributions due to condensation alone. …

Microscopic And Laboratory Scale Characterization Methods To Evaluate Biomass Deconstruction, Meenaa Chandrasekar

Dissertations, Master's Theses and Master's Reports

Renewable fuels from lignocellulosic biomass are an appealing option because they can seamlessly integrate into the existing fuel distribution infrastructure. Lignocellulosic biomass constitutes nonedible plant material obtained from plant cell walls. The natural recalcitrance of lignocellulosic biomass poses a challenge in accessing the cell wall carbohydrates during biochemical conversion. Despite various approaches, enzymatic hydrolysis of lignocellulosic biomass remains economically impractical due to incomplete knowledge about biomass recalcitrance and the influence of environmental factors on biomass quality.

The first goal of this dissertation was to construct a microfluidic imaging reactor to better understand the tissue-specific deconstruction of plant materials. Confocal laser …

Prediction Of Sumoylation Sites In Proteins From Language Model Representations, Evgenii Sidorov

Dissertations, Master's Theses and Master's Reports

Sumoylation is an essential post-translational modification intimately involved in a diverse range of eukaryotic cellular mechanisms and plays a significant role in DNA repair. Some researchers hypothesize that a high level of SUMOylation events in cancer cells improves cells' chances for survival under stress conditions by regulating tumor-related proteins.

This study belongs to a booming field of harnessing computational power to the domain of life. Prediction of protein structure, its molecular function, and the design of new drugs are just a few examples of the applications within this exciting area of research. By leveraging computational power, researchers can analyze vast …

Integrating Lidar, Aeromagnetic, And Geological Field Data To Identify Structural-Lithologic Elements Within The Archean Carney Lake Gneiss Complex, Ian M. Gannon

Dissertations, Master's Theses and Master's Reports

The Archean Carney Lake Gneiss Complex (CLGC) is a suite of rocks on the southern margin of the Superior Province and within the Minnesota River Valley subprovince. It is one of many gneissic domes in Michigan, Wisconsin, and Minnesota whose complex geology is poorly understood due to a previous lack of economic interest in gneissic terranes and significant challenges to their investigation. As a consequence, the CLGC and other gneissic domes in the region are represented on geologic maps as undifferentiated masses surrounded by better defined and understood Paleoproterozoic supracrustal rocks and igneous intrusions. Within the last ten years, however, …

Impacts Of Introduced Earthworms On Sugar Maple Sap Chemistry And The Discourse Of Invasion Ecology, Shelby Nicole Lane-Clark

Dissertations, Master's Theses and Master's Reports

To delve into further understanding of the relationships between introduced earthworms, forest ecosystems, and the human systems they impact, two approaches were taken. In the first approach, we seek to explore ways we can shift the discourse within and regarding the field of invasion ecology by re-framing how we approach discussion, management, and education regarding introduced species. Language issues surrounding introduced species through the example of earthworms in North America are described and potential solutions are provided, including a repositioned perspective that may facilitate better relationships with the natural world. The goal is to shift the language to be more …

Fine Root Respiration In Quercus Rubra Is Influenced By Multidimensional Trade-Offs In Root Trait Space, Emma L. Shedd

Dissertations, Master's Theses and Master's Reports

Economic theory is often applied to plant strategies, arguing that trade-offs exist between maximizing quick returns on investment within short lifespans or producing longer-lived tissues with slower returns. While these trade-offs are well-studied aboveground, their influence on belowground traits, including root respiration, remains unclear. Resource heterogeneity and symbioses with mycorrhizal fungi may complicate root trade-off axes beyond those predicted by economics theory alone. Investigating fine root trade-offs in Quercus rubra populations along a latitudinal gradient in the Midwest, USA, we identified two distinct axes of variation in root traits: one related to a potential “fungal symbiosis” trade-off and another representing …

Near-Surface Soil Nitrogen And Vegetation Response To Invasive Emerald Ash Borer In Forested Black Ash Wetlands Of The Western Upper Peninsula, Michigan, Usa, Daniel A. Beyer

Dissertations, Master's Theses and Master's Reports

Invasive emerald ash borer (EAB) (Agrilus planipennis Fairmaire) poses an imminent threat to the structure and function of North American hardwood forests, particularly black ash (Fraxinus nigra Marshall), and alters the hydrologic and ecological services of their wetlands. Black ash trees regularly grow in seasonally saturated soils and are responsible for hydrologic regulation and nutrient cycling. In this study, a gradient of black ash wetlands impacted by EAB were monitored to assess vegetation changes and near-surface soil nitrogen availability. Vegetation community changes were intertwined with nitrogen cycle disturbances following EAB infestation. As black ash died and fell to …

Uhplc/Ft-Ms Non-Targeted Screening Approach For Biomass Burning Organic Aerosol And Liquid Smoke As Biomass Burning Organic Aerosol Surrogate, D.M.R. Thusitha Dinusha Kumarihami Divisekara

Dissertations, Master's Theses and Master's Reports

Biomass combustion contributes a large amount of aerosol particles to the atmosphere impacting the climate system and human health. Identifying the molecular composition of biomass burning organic aerosol (BBOA) is challenging because of its complex nature. Liquid chromatography mass spectrometric non-targeted screening (NTS) methods provide a comprehensive analysis. However, the instrumentation and data analysis are challenging due to the extreme complexity of environmental samples. In the first part of this study, we implemented a new LC/FT-MS data processing approach by combining existing open-source data processing tools, MZmine2.53 and MFAssignR. The method's validity was checked using the standard polyacrylic acid (PAA) …

Remote Sensing Approach For Terramechanics Applications Utilizing Machine And Deep Learning, Jordan J. Ewing

Dissertations, Master's Theses and Master's Reports

Terrain traversability is critical for developing Go/No Go maps, significantly impacting a mission's success. To predict the mobility of a vehicle over a terrain, one must understand the soil characteristics. In situ measurements performed by soldiers in the field are the current method of collecting this information, which is time-consuming, are only point measurements, and can put soldiers in harm's way. Therefore, this study investigates using remote sensing as an alternative approach to characterize terrain properties.

This approach will explore the relationships between electromagnetic radiation and soil types with varying properties. Optical, thermal, and hyperspectral sensors will be used to …

Rain-Induced Hazards In Remote, Low-Resource Communities: A Case Study Of Flash Flooding In The Usulután Department, El Salvador, Natalea Cohen

Dissertations, Master's Theses and Master's Reports

Rain-induced natural hazards can lead to devastating and potentially life-threatening impacts. Understanding areas susceptible to flash flooding and characterizing the intensity of flash flood events is critical in improving the mitigation and emergency preparedness of vulnerable communities. Flash floods occur on small spatial scales and for short durations making it challenging to classify flash flood susceptibility and forecast events. Modeling flash flooding becomes even more difficult when focusing on data-poor regions. This study is based in California, El Salvador, an agricultural community located in the Central American Dry Corridor (CADC), a region experiencing the impacts of climate change and associated …

The Art And Science Of Wood: From Pyrography To Termites And Wood Decomposition, Joanna D. Walitalo

Dissertations, Master's Theses and Master's Reports

Wood is vital to many natural ecosystems, as it provides energy, nutrients, and habitat for organisms from the micro- to the macro- scale. Wood is also critical to humans for similar reasons, and can be an important medium of art and education. This dissertation addresses three diverse aspects of wood with the contexts of science, art, and education. First, we explored the impact of timber harvest techniques and site preparation on microbial wood decay and subterranean termite responses on a forest-stand scale. The amount of coarse woody debris removed post-harvest, coupled with the location and species of the test wood …

Sequencing Antisense Transcriptome Of Populus Through Antisense/Sense Transcript Pair Enrichment, Emma V. Burke

Dissertations, Master's Theses and Master's Reports

Past transcriptome research on plants focused primarily on protein coding genes, and only recently researchers began looking into the non-protein coding regions that may play significant roles in gene regulation. Antisense RNA transcripts that are found naturally in the cell share complementary sequence with sense transcripts and have been shown to regulate expression of their sense counterparts. Since antisense RNA has been largely under-studied and difficult to sequence because of their low relative abundance, new methods are needed to target antisense RNA for efficient genome-wide profiling. To address this gap in methods to efficiently and cost effectively enrich antisense RNA …

Study Of Nanocomposite Materials Using Molecular Dynamics, Prashik Sunil Gaikwad

Dissertations, Master's Theses and Master's Reports

There is an increase in demand for new lightweight structural materials in the aerospace industry for more efficient and affordable human space travel. Polymer matrix composites (PMCs) with reinforcement material as carbon nanotubes (CNTs) have shown exceptional increase in the mechanical properties. Flattened carbon nanotubes (flCNTs) are a primary component of many carbon nanotube (CNT) yarn and sheet materials, which are promising reinforcements for the next generation of ultra-strong composites for aerospace applications. These flCNT/polymer materials are subjected to extreme pressure and temperature during curing process. Therefore there is a need to investigate the evolution of properties during the curing …

Knowledge Discovery On The Integrative Analysis Of Electrical And Mechanical Dyssynchrony To Improve Cardiac Resynchronization Therapy, Zhuo He

Dissertations, Master's Theses and Master's Reports

Cardiac resynchronization therapy (CRT) is a standard method of treating heart failure by coordinating the function of the left and right ventricles. However, up to 40% of CRT recipients do not experience clinical symptoms or cardiac function improvements. The main reasons for CRT non-response include: (1) suboptimal patient selection based on electrical dyssynchrony measured by electrocardiogram (ECG) in current guidelines; (2) mechanical dyssynchrony has been shown to be effective but has not been fully explored; and (3) inappropriate placement of the CRT left ventricular (LV) lead in a significant number of patients.

In terms of mechanical dyssynchrony, we utilize an …

Changes In Poplar Digestibility Due To Overexpression Of Laccase Genes, Fnu Arunima

Dissertations, Master's Theses and Master's Reports

Woody biomass, particularly poplar, is a sought-after feedstock for lignocellulosic biofuels because of its year-round availability and high productivity. Because of cell wall components like lignin, poplar is inherently resistant to enzymatic saccharification. The experiment aims to determine whether the controlled overexpression of laccase genes alters the biomass composition and whether it can create poplar that is more digestible than the wildtype.

Three sets of transgenic poplar (Populus tremula x alba) samples were evaluated: laccase 18, laccase 27, and STTM. Laccase 18 samples, an target of miRNA397a was overexpressed along with Laccase 27. Short Tandem target Mimic (STTM) technology …

Treating Contaminated Water: Predicting Reactivities Of Aqueous Organic Contaminants With Hydrated Electrons, And Development Of A Biogarden To Manage Greywater Discharge In Monteverde, Costa Rica, Rose C. Daily

Dissertations, Master's Theses and Master's Reports

Advanced reduction processes (ARPs) that generate highly reactive solvated electrons (e^-_aq) are a promising method for the destruction of conventional and emerging aqueous organic contaminants. While there is a large database of contaminant reactivity with e^-_aq in the literature, there is little information on the detailed elementary mechanisms for reduction of multifunctional group compounds and the impact of those functional groups on reactivity. As it is difficult to determine specific mechanisms through experiments and time consuming to measure reactivity, the development of computational approaches to elucidate mechanisms and predict reactivity is becoming increasingly important. In …