Digital Commons Network^™

Aptamer-Based Proteomics Measuring Preoperative Cerebrospinal Fluid Protein Alterations Associated With Postoperative Delirium, Simon T. Dillon, Sarinnapha M. Vasunilashorn, Hasan H. Otu, Long Ngo, Tamara Fong, Xuesong Gu, Michele Cavallari, Alexandra Touroutoglou, Mouhsin Shafi, Sharon K. Inouye, Zhongcong Xie, Edward R. Marcantonio, Towia A. Libermann

Department of Electrical and Computer Engineering: Faculty Publications

Delirium is a common postoperative complication among older patients with many adverse outcomes. Due to a lack of validated biomarkers, prediction and monitoring of delirium by biological testing is not currently feasible. Circulating proteins in cerebrospinal fluid (CSF) may reflect biological processes causing delirium. Our goal was to discover and investigate candidate protein biomarkers in preoperative CSF that were associated with the development of postoperative delirium in older surgical patients. We employed a nested case–control study design coupled with high multiplex affinity proteomics analysis to measure 1305 proteins in preoperative CSF. Twenty-four matched delirium cases and non-delirium controls were selected …

Implications Of Bhagvad Gita On Library System In Digital Era: A Philosophical View, Subir Kumar

Library Philosophy and Practice (e-journal)

Bhagvad Gita has potential of spiritual awakening and intellectual stimulation. Bhagvad Gita is the science of Wisdom, Moral Values and Celestial Consciousness. Bhagvad gita is the sublime philosophy of life. Gita has capability to promote spiritual and constructive work culture in the library system. Gita provides positive coping mechanism among the learners.

Lord Krishna advocated righteous action, righteous intention and righteous direction. Gita is more than a religion, but is the real philosophy and practice of life. It is capable of enhancing the professional flexibility and compatibility among the library staff.

Bhagvad Gita potentially strengthens the pillars of professional library …

The Impact Of Insomnia Symptoms On Obesity Among Mexicans Aged 50 And Older, Kanwal N Momin, Connor Sheehan, Rafael Samper-Ternent, David S López, Rebeca Wong, Sadaf Arefi Milani

Faculty, Staff and Student Publications

OBJECTIVE: To examine the association between insomnia and obesity in Mexican adults aged 50 and older.

MATERIALS AND METHODS: We used data from the Mexican Health and Aging Study (2015-2018). Self-reported insomnia was measured using the modified insomnia severity index with scores ranging from zero to six. Obesity was categorized using body mass index (BMI ≥ 30 kg/m2). We used generalized estimating equations to assess the association between insomnia and obesity over three years.

RESULTS: Insomnia was associated with obesity (odds ratio [OR]: 1.06, 95% confidence interval [CI]: 1.01,1.11), among those with no obesity at baseline. Among those with obesity, …

Neurocognitive Outcomes In Adult Survivors Of Neuroblastoma: A Report From The Childhood Cancer Survivor Study, Caroline Hesko, Wei Liu, Deo K Srivastava, Tara M Brinkman, Lisa Diller, Todd M Gibson, Kevin C Oeffinger, Wendy M Leisenring, Rebecca Howell, Gregory T Armstrong, Kevin R Krull, Tara O Henderson

Faculty, Staff and Student Publications

Background: Despite survival improvements, there is a paucity of data on neurocognitive outcomes in neuroblastoma survivors. This study addresses this literature gap.

Methods: Neurocognitive impairments in survivors were compared to sibling controls from the Childhood Cancer Survivor Study (CCSS) using the CCSS Neurocognitive Questionnaire. Impaired emotional regulation, organization, task efficiency, and memory defined as scores ≥90th percentile of sibling norms. Modified Poisson regression models evaluated associations with treatment exposures, era of diagnosis, and chronic conditions. Analyses were stratified by age at diagnosis (≤1 and >1 year) as proxy for lower versus higher risk disease.

Results: Survivors (N = 837; median …

A Phenome-Wide Association Study (Phewas) Of Late Onset Alzheimer Disease Genetic Risk In Children Of European Ancestry At Middle Childhood: Results From The Abcd Study®, Aaron Gorelik

Arts & Sciences Graduate Student Theses and Dissertations

Genetic risk for Late Onset Alzheimer Disease (AD) has been associated with lower cognition and smaller hippocampal volume in healthy young adults. However, whether these and other associations are present during childhood remains unclear. Using data from 5,556 genomically-confirmed European ancestry youth who completed the baseline session of the ongoing the Adolescent Brain Cognitive Development^SM Study (ABCD Study^®), our phenome-wide association study estimating associations between four indices of genetic risk for late-onset AD (i.e., AD polygenic risk scores (PRS), APOE rs429358 genotype, AD PRS with the APOE region removed (AD_PRS-APOE), and an interaction between AD …

Masculinity And Gender Roles In Selected Stories By Haruki Murakami, Maha Hamed Issa

Alustath Journal for Human and Social Sciences

Haruki Murakami (1949) is a Japanese novelist who uses fiction to portray modern man's condition. Sexual identity, loneliness, and nostalgia are common themes in his works. Men without Women (2014) is a collection of short stories in which Murakami reverses gender roles to show men's desperate seek for companionship in their lives. According to Hans Robert Jauss (1921-1997), a German literary theorist, there is no identical interpretation of any literary text. Yet, a dialogic correspondence between the text and the reader allows the reader's voice to breathe different life into the text. Therefore this study aims to illustrate the identity …

Psychological And Physical Violence In Darkly Dreaming Dexter By Jeff Lindsay, Omar Abbas Sallal

Alustath Journal for Human and Social Sciences

This study aims to examine the psychological and physical violence in Jeff Lindsay’s Darkly Dreaming Dexter. This study analyzed the psychological and social influences on serial killers by using two theories; Milgram’s “Obedience Experiment”, the experiment proved that the majority of people will obey the instructions that came from higher authority without thinking about moral values. The second theory is Sigmund Freud's beliefs about violence and right he thought that violence is a natural thing and there is a strong relationship between violence and right. The research samples are the serial killers and their victims and how theories put forward …

Using Remote Sensing And Geographic Information Systems To Detect Desertified Agricultural Lands In Al-Qadisiyah Governorate, Abbas Hashem Khaled

Alustath Journal for Human and Social Sciences

Desertification is one of the phenomena that threaten the ecological system، otherwise economic and social. This study aims to assess and control desertification in Al-Qadisiyah Governorate through the use of remote sensing techniques and geographic information systems.Vegetation Index (NDVI) and Crust Index (CI) were used، which were applied from both (OII) and Landsat (ETM) satellite images during the years 2013 and 2020 . The results showed that the total area of vegetation cover in 2013 was 2902 square kilometers . With a percentage of 32.30% of the total area، which made it classified in that year within the medium desertification? …

Direct Modulators Of K-Ras–Membrane Interactions, Johannes Morstein, Rebika Shrestha, Que N Van, César A López, Neha Arora, Marco Tonelli, Hong Liang, De Chen, Yong Zhou, John F Hancock, Andrew G Stephen, Thomas J Turbyville, Kevan M Shokat

Faculty, Staff and Student Publications

Protein-membrane interactions (PMIs) are ubiquitous in cellular signaling. Initial steps of signal transduction cascades often rely on transient and dynamic interactions with the inner plasma membrane leaflet to populate and regulate signaling hotspots. Methods to target and modulate these interactions could yield attractive tool compounds and drug candidates. Here, we demonstrate that the conjugation of a medium-chain lipid tail to the covalent K-Ras(G12C) binder MRTX849 at a solvent-exposed site enables such direct modulation of PMIs. The conjugated lipid tail interacts with the tethered membrane and changes the relative membrane orientation and conformation of K-Ras(G12C), as shown by molecular dynamics (MD) …

A Phase I, Multicenter, Open-Label, First-In-Human Study Of Ds-6157a In Patients With Advanced Gastrointestinal Stromal Tumor, Suzanne George, Peter Oppelt, Et Al.

2020-Current year OA Pubs

PURPOSE: To evaluate DS-6157a, an antibody-drug conjugate targeting G protein-coupled receptor 20 (GPR20), in gastrointestinal stromal tumors (GIST).

PATIENTS AND METHODS: In this phase I multicenter, open-label, multiple-dose study, patients with previously treated advanced GIST received intravenous DS-6157a on Day 1 of 21-day cycles, with a starting dose of 1.6 mg/kg. The primary objective evaluated the safety and tolerability of DS-6157a, while determining dose-limiting toxicity (DLT) and the MTD. Secondary objectives included plasma pharmacokinetics parameters, plasma antidrug antibodies (ADA), and efficacy.

RESULTS: A total of 34 patients enrolled. DS-6157a was well tolerated, with DLTs in 4 patients (11.8%) at doses …

Protocol For Isolating Human Bcam-Positive Corneal Progenitor Cells By Flow Cytometry And Cell Sorting, Yuzuru Sasamoto, Philip C. Yeung, Johnathan Tran, Markus H. Frank, Natasha Y. Frank

Research outputs 2022 to 2026

BCAM-positive basal limbal epithelial cells are an early transit-amplifying cell population (TAC) capable of holoclone formation and corneal epithelial differentiation. Here, we present a protocol for isolating BCAM-positive cells from human donor corneas by flow cytometry and cell sorting. We describe steps for cell dissection and dissociation, antibody staining, and flow cytometry. We then detail procedures for culturing the purified BCAM-positive and BCAM-negative cells for holoclone and cell sheet formation assays to study the factors that regulate corneal regeneration. For complete details on the use and execution of this protocol, please refer to Sasamoto et al.1

Decoding Depression Severity From Intracranial Neural Activity, Jiayang Xiao, Nicole R Provenza, Joseph Asfouri, John Myers, Raissa K Mathura, Brian Metzger, Joshua A Adkinson, Anusha B Allawala, Victoria Pirtle, Denise Oswalt, Ben Shofty, Meghan E Robinson, Sanjay J Mathew, Wayne K Goodman, Nader Pouratian, Paul R Schrater, Ankit B Patel, Andreas S Tolias, Kelly R Bijanki, Xaq Pitkow, Sameer A Sheth

Faculty, Staff and Students Publications

BACKGROUND: Disorders of mood and cognition are prevalent, disabling, and notoriously difficult to treat. Fueling this challenge in treatment is a significant gap in our understanding of their neurophysiological basis.

METHODS: We recorded high-density neural activity from intracranial electrodes implanted in depression-relevant prefrontal cortical regions in 3 human subjects with severe depression. Neural recordings were labeled with depression severity scores across a wide dynamic range using an adaptive assessment that allowed sampling with a temporal frequency greater than that possible with typical rating scales. We modeled these data using regularized regression techniques with region selection to decode depression severity from …

Recent Breakthroughs In Using Quantum Dots For Cancer Imaging And Drug Delivery Purposes, Aisha Hamidu, William G. Pitt, Ghaleb A. Husseini

Faculty Publications

Cancer is one of the leading causes of death worldwide. Because each person’s cancer may be unique, diagnosing and treating cancer is challenging. Advances in nanomedicine have made it possible to detect tumors and quickly investigate tumor cells at a cellular level in contrast to prior diagnostic techniques. Quantum dots (QDs) are functional nanoparticles reported to be useful for diagnosis. QDs are semiconducting tiny nanocrystals, 2–10 nm in diameter, with exceptional and useful optoelectronic properties that can be tailored to sensitively report on their environment. This review highlights these exceptional semiconducting QDs and their properties and synthesis methods when used …

Hematopoietic Stem And Progenitor Cells Confer Cross-Protective Trained Immunity In Mouse Models, Bailee N Kain, Brandon T Tran, Pamela N Luna, Ruoqiong Cao, Duy T Le, Marcus A Florez, Laure Maneix, Jack D Toups, Daniel E Morales-Mantilla, Scott Koh, Hyojeong Han, Roman Jaksik, Yun Huang, Andre Catic, Chad A Shaw, Katherine Y King

Duncan NRI Faculty and Staff Publications

Recent studies suggest that infection reprograms hematopoietic stem and progenitor cells (HSPCs) to enhance innate immune responses upon secondary infectious challenge, a process called “trained immunity.” However, the specificity and cell types responsible for this response remain poorly defined. We established a model of trained immunity in mice in response to Mycobacterium avium infection. scRNA-seq analysis revealed that HSPCs activate interferon gamma-response genes heterogeneously upon primary challenge, while rare cell populations expand. Macrophages derived from trained HSPCs demonstrated enhanced bacterial killing and metabolism, and a single dose of recombinant interferon gamma exposure was sufficient to induce similar training. Mice transplanted …

Spatial And Temporal Distribution Of Phalaropes (Phalaropus Spp.) And Adult Brine Flies (Ephydra Spp.) Are Linked On Great Salt Lake, Utah, Maureen G. Frank, Michael R. Conover

Western North American Naturalist

Great Salt Lake (GSL), Utah, is home to some of the world’s largest concentrations of Wilson’s Phalaropes (Phalaropus tricolor), Red-necked Phalaropes (Phalaropus lobatus), American Avocets (Recurvirostra americana), and Black-necked Stilts (Himantopus mexicanus). These birds spend several weeks on GSL feeding on larvae, pupae, and adult brine flies (Ephydra spp.) before migrating to their wintering grounds in Central and South America. Unfortunately, GSL is shrinking in size and becoming more saline due to water diversions and climate change. Assessing how a smaller and more saline GSL will affect adult brine flies is …

Final 2023 Butte Priority Soils Operable Unit (Bpsou) Borrow Amendment Work Plan, Pioneer Technical Services, Inc.

Silver Bow Creek/Butte Area Superfund Site

No abstract provided.

Transcriptomic And Proteomic Assessment Of Tocilizumab Response In A Randomized Controlled Trial Of Patients Hospitalized With Covid-19, Haridha Shivram, Jason A Hackney, Carrie M Rosenberger, Anastasia Teterina, Aditi Qamra, Olusegun Onabajo, Jacqueline Mcbride, Fang Cai, Min Bao, Larry Tsai, Aviv Regev, Ivan O Rosas, Rebecca N Bauer

Faculty, Staff and Students Publications

High interleukin (IL)-6 levels are associated with greater COVID-19 severity. IL-6 receptor blockade by tocilizumab (anti-IL6R; Actemra) is used globally for the treatment of severe COVID-19, yet a molecular understanding of the therapeutic benefit remains unclear. We characterized the immune profile and identified cellular and molecular pathways modified by tocilizumab in peripheral blood samples from patients enrolled in the COVACTA study, a phase 3, randomized, double-blind, placebo-controlled trial of the efficacy and safety of tocilizumab in hospitalized patients with severe COVID-19. We identified markers of inflammation, lymphopenia, myeloid dysregulation, and organ injury that predict disease severity and clinical outcomes. Proteomic …

Viral Serpins-A Family Of Highly Potent Immune-Modulating Therapeutic Proteins, Kyle S. Varkoly, Roxana N. Beladi, Mostafa Hamada, Grant Mcfadden, James Irving, Alexandra R. Lucas

Student Publications

Serine protease inhibitors, SERPINS, are a highly conserved family of proteins that regulate serine proteases in the central coagulation and immune pathways, representing 2-10% of circulating proteins in the blood. Serine proteases form cascades of sequentially activated enzymes that direct thrombosis (clot formation) and thrombolysis (clot dissolution), complement activation in immune responses and also programmed cell death (apoptosis). Virus-derived serpins have co-evolved with mammalian proteases and serpins, developing into highly effective inhibitors of mammalian proteolytic pathways. Through interacting with extracellular and intracellular serine and cysteine proteases, viral serpins provide a new class of highly active virus-derived coagulation-, immune-, and apoptosis-modulating …

Structure, Function, And Molecular Landscapes Of The Aging Retina, Jeffrey D Zhu, Sharma Pooja Tarachand, Qudrat Abdulwahab, Melanie A Samuel

Faculty, Staff and Students Publications

Because the central nervous system is largely nonrenewing, neurons and their synapses must be maintained over the lifetime of an individual to ensure circuit function. Age is a dominant risk factor for neural diseases, and declines in nervous system function are a common feature of aging even in the absence of disease. These alterations extend to the visual system and, in particular, to the retina. The retina is a site of clinically relevant age-related alterations but has also proven to be a uniquely approachable system for discovering principles that govern neural aging because it is well mapped, contains diverse neuron …

Physical And Chemical Effects Of Conventional Microplastic Glitter Versus Alternative Glitter Particles On A Freshwater Plant (Lemnaceae: Lemna Minor), Bas Boots, Dannielle Senga Green, Brigitta Olah-Kovacs, Falco F De, Emanuele Lupo

School of Geography, Earth and Environmental Sciences

Glitters are primary microplastics which are directly littered into the environment, yet the ecological effects have seldom been tested. When microplastics enter the environment, their physical presence and chemical leachate may alter the physiology of primary producers. Glitter can be composed of plastic or natural and/or biodegradable materials, often with additives. Three experiments were run for 14 days to separate chemical and physical effects of different types of glitter: polyethylene terephthalate (PET), biodegradable modified regenerated cellulose (MRC), synthetic mica, and a natural particle control (kaolinite) on several physical characteristics of Lemna minor (common duckweed). L. minor was exposed to either …

Velocity-Based Training—A Critical Review, Stuart Guppy, Kristina Kendall, G. Gregory Haff

Research outputs 2022 to 2026

Velocity-based training (VBT) is an increasingly popular programming strategy used by strength and conditioning professionals to develop their athlete's ability to express force rapidly. To implement the varying forms of VBT effectively within their training regimes, strength and conditioning professionals need to understand the strengths and weaknesses of strategies, such as predicting 1 repetition maximum using the load-velocity profile, modulating training loads using the load-velocity profile, and controlling training volume using the magnitude of velocity-loss. The aim of this review was to highlight these strengths and weaknesses and then provide practical examples of when each programming strategy may be most …

Single Cell Multiomics Identifies Cells And Genetic Networks Underlying Alveolar Capillary Dysplasia, Minzhe Guo, Kathryn A Wikenheiser-Brokamp, Joseph A Kitzmiller, Cheng Jiang, Guolun Wang, Allen Wang, Sebastian Preissl, Xiaomeng Hou, Justin Buchanan, Justyna A Karolak, Yifei Miao, David B Frank, William J Zacharias, Xin Sun, Yan Xu, Mingxia Gu, Pawel Stankiewicz, Vladimir V Kalinichenko, Jennifer A Wambach, Jeffrey A Whitsett

Faculty, Staff and Students Publications

Rationale

Alveolar capillary dysplasia with misalignment of pulmonary veins (ACDMPV) is a lethal developmental disorder of lung morphogenesis caused by insufficiency of FOXF1 (forkhead box F1) transcription factor function. The cellular and transcriptional mechanisms by which FOXF1 deficiency disrupts human lung formation are unknown.

Objectives

To identify cell types, gene networks, and cell–cell interactions underlying the pathogenesis of ACDMPV.

Methods

We used single-nucleus RNA and assay for transposase-accessible chromatin sequencing, immunofluorescence confocal microscopy, and RNA in situ hybridization to identify cell types and molecular networks influenced by FOXF1 in ACDMPV lungs.

Measurements and Main Results

Pathogenic single-nucleotide variants and copy-number …

A High-Throughput Protocol For Measuring Solution Ph Of Bacterial Cultures Using Uv-Vis Absorption Spectrophotometry, Kristen A Engevik, Hyland Gonzalez, Clay Daniels, Ronald K Stavert, Numan Oezguen, Melinda A Engevik, Thomas D Horvath

Faculty, Staff and Students Publications

We present a protocol for measuring the pH of cell-free bacterial-conditioned media based on changes in the ultraviolet-visible (UV-Vis) absorbance spectrum using the pH indicator dye litmus. This protocol includes detailed procedures for performing bacterial culturing, examining bacterial growth, collecting cell-free supernatant, litmus dye addition, and pH-based calibration curve preparations. This assay has been designed for flexible formatting that can accommodate both high-volume and low-volume sample sets.

Ifn-Λ Derived From Nonsusceptible Enterocytes Acts On Tuft Cells To Limit Persistent Norovirus, Harshad Ingle, Heyde Makimaa, Somya Aggarwal, Hongju Deng, Lynne Foster, Yuhao Li, Elizabeth A Kennedy, Stefan T Peterson, Craig B Wilen, Sanghyun Lee, Mehul S Suthar, Megan T Baldridge

2020-Current year OA Pubs

Norovirus is a leading cause of epidemic viral gastroenteritis, with no currently approved vaccines or antivirals. Murine norovirus (MNoV) is a well-characterized model of norovirus pathogenesis in vivo, and persistent strains exhibit lifelong intestinal infection. Interferon-λ (IFN-λ) is a potent antiviral that rapidly cures MNoV. We previously demonstrated that IFN-λ signaling in intestinal epithelial cells (IECs) controls persistent MNoV, and here demonstrate that IFN-λ acts on tuft cells, the exclusive site of MNoV persistence, to limit infection. While interrogating the source of IFN-λ to regulate MNoV, we confirmed that MDA5-MAVS signaling, required for IFN-λ induction to MNoV in vitro, controls …

Factors Influencing Remote Working For Facilitators In Under Resourced Universities, Anelisa Tyutyu, Salah Kabanda, Guidance Mthwazi

African Conference on Information Systems and Technology

Remote working has been widely implemented in industry. In higher education, a gap exists in understanding how it has been implemented, particularly in under resourced universities. The purpose of this study was to explain the factors that influence remote working experiences of higher education facilitators (HEFs) in under resourced higher education institutions (HEIs). With the use of South African HEIs that are classified as historically disadvantaged, the study explained how remote working has been implemented and experienced and identified strategies that were shown to work in under resourced universities. A deductive approach to theory and an interpretivist research philosophy were …

Experiences Of African Women In Stem Careers: A Systematic Literature Review., Kaluwa Siwale, Gwamaka Mwalemba, Ulrike Rivett

African Conference on Information Systems and Technology

The discourse on women's underrepresentation in science, technology, engineering, and mathematics (STEM) mainly centres on the global north, leaving a gap in understanding the perspectives of African women in STEM. To address this, a systematic literature review was conducted to explore African women's experiences in STEM careers and education. After applying inclusion and criteria, 18 published articles were analysed. 8 key issues emerge: work environment, education system, work-life balance, gender-based stereotypes, racial bias, sexual harassment, inadequate support/mentorship, and self-imposed limits. These themes intertwine, with some aspects influencing others. Grasping the complexities and interactions of these factors provides insights into challenges …

E-Wild Life Alert: Tackling The Human-Wildlife Conflict Problem, Eliel Kundai Zhuwankinyu, Sibonile Moyo, Catherine Chivasa, Smart Ncube

African Conference on Information Systems and Technology

Depletion of resources meant for both human and animal survival leads to competition for these. Human-wildlife conflict (HWC) occurs when these two parties compete for resources such as space, water, and food. If not properly managed, HWC can lead to loss of livelihoods and even loss of life. This paper discusses the design and development of an E-Wildlife Alert application that uses machine learning to detect dangerous animals. Using the Design Science Research method, a convolutional neural network is trained to build an artifact that detects five dangerous animals from an African context. The artifact is mounted on a robot …

Feed Formulation: Expanding Our Horizons Beyond The Simple Provision Of Least-Cost Diets, Rick Kleyn, Mariana Ciacciariello

Proceedings of the Arkansas Nutrition Conference

Commercial feed formulation is typically based on linear programming (LP) algorithms that derive a least-cost solution for a blend of ingredients that meet specific nutrient criteria. This methodology has been effectively used for decades, but how we achieve our primary goal, which is sustainable poultry production, needs to be reconsidered in broader terms. Some of the issues that this paper will deal with are: not all parameters that we consider are linear and additive; we are still unsure of how to build the matrix required for feed formulation; dealing with the variability encountered in every batch of ingredient remains a …

No Antibiotic Ever (Nae) Versus Conventional Broiler Production: It’S Complicated, Marcos H. Rostagno

Proceedings of the Arkansas Nutrition Conference

Broiler production has evolved significantly over the years to meet a constant growing demand for animal protein. Rooted on major advances in genetics, facilities and environmental conditions, management, and specially, in nutrition and health, the U.S. broiler industry improved tremendously in productivity and efficiency, clearly demonstrated by a marked reduction of the average market age (i.e., days to market), while significantly increasing average market weight, breast meat yield and feed efficiency (National Chicken Council, 2023). For decades, conventional broiler production was dominant, with extensive use of antibiotics to promote growth and manage disease challenges (prevention and control). However, concerns over …

Tobacco, Alcohol And Diet As Mortality Risk Factors: The Secondary Analysis Of The 25-Year Cohort Study, Alina Egorova, Bulat Idrisov, Romany Redman, Stanislav Otstavnov, Sergey Soshnikov

Journal of Health Research

Background: Individual lifestyle risk factors have been linked to increased mortality globally; however, there is limited data on these associations in Russia. A secondary analysis of the Russia Longitudinal Monitoring Survey (RLMS) data was conducted to close this gap.

Methods: The secondary data have been obtained from a nationally-representative annual cohort survey conducted by the Higher School of Economics (HSE). In this original study, for the first time in Russia, we extracted RRs for researched risk factors. Of additional original value, we made a prospective-retrospective cohort based on a representative longitudinal survey and provided the deaths as outcomes for survival …