Digital Commons Network^™

Cell-Based Experimental Evidence To The Anti-Covid-19 Potential Of Ashwagandha And Honeybee Propolis Ingredients, Renu Wadhwa, Durai Sundar, Yoshiyuki Ishida, Keiji Terao, Sunil C. Kaul

Research Symposium

Background: The COVID-19 pandemic emerged in December 2019 by a novel strain of SARS-CoV-2 (Severe Acute Respiratory Syndrome Coronavirus 2) has led to new endeavours in repurposing of existing drugs, anti-COVID-19 vaccine and drug development. Natural products, due to their general safety and wider availability, have attracted research and public attention. In this study, we report anti-COVID potential of compounds from honeybee propolis and Ayurvedic herb, Ashwagandha. Effect of active ingredients was studied on human cell surface receptors (ACE-2:Angiotensin Converting Enzyme-2/Spike protein and TMPRSS2:Transmembrane Protease Serine 2), critical for virus infection and virus main protease (Mpro, essential for virus replication), …

Dpyd Pathogenic Variants Associated With Fluoropyrimidines Toxicity, Diana Cristina Pérez-Ibave, Noé Israel Oliva-García, Irasema Ramos-Martínez, Francisco Javier Villarreal Alvarado, Valeria Jimena Gómez Ordaz, Jonatán Isaí Cortes Alfaro, Carlos Horacio Burciaga-Flores, Juan Francisco González-Guerrero, Oscar Vidal-Gutiérrez, Maria De Lourdes Garza-Rodriguez

Research Symposium

Background: Genetic variants in dihydropyrimidine dehydrogenase gene (DPYD) coding for the key enzyme (DPD) of fluoropyrimidines (FPs) catabolism. DPYD contributes to the development of severe FPs-related toxicity, and pathogenic DPYD variants detection reduces side effects and complications associated with FP-toxicity. The allelic frequency of these variants in the Mexican population is currently unknown.

Methods: The study was carried out at the Centro Universitario Contra el Cáncer (CUCC) of the Universidad Autónoma de Nuevo León (UANL) in Monterrey México. Genomic DNA was isolated from 154 subjects using the QIAamp DNA Blood Midi kit (QIAGEN) following the manufacturer's recommendations. We …

Teaching To Develop Perspective, Skills, Confidence, And Identity As Problem-Solving Engineers, Russell Kirk Pirlo

Research and Reflection on Learning and Teaching in Higher Education

The “core” of an engineering degree program typically comprises the concepts, equations, and technical skills needed, as well as their practical application to common problems of the profession. This core is then divided into the “content” that must be covered in each course. It is widely recognized, however, that successful individuals do not thrive as professionals on content alone. Thus, there is significant and increasing emphasis across higher education to “educate the whole person.” These efforts aim to develop “deep” qualities like grit, critical thinking, perseverance, learning from failure, valuing diversity, teamwork, leadership, curiosity, recognizing opportunity, creating value, and acting …

The Impact Of Social Isolation On The Neural Pathways Of Dopamine Neurons In The Ventral Tegmental Area (Vta) And The Nucelus Accumbens (Nac): Implications For The Treatment Of Depression, Anxiety, And Drug Addiction., Cristian M. Botello, Sasawan Heingraj, John L. Vandeberg, Mario Gil

Research Symposium

As the literature on the Monodelphis domestica continues to grow, it is important to contribute to the knowledge base regarding neural pathways and their role in social behavior in this species. Previous studies have provided evidence that increased activity in ventral tegmental area (VTA) dopamine neurons were associated with more social activity in mice. It is also known that in traditional rodent models, the Nucleus Accumbens (NAcc) is implicated in interaction reward processes like motivation; however, more research is needed to elucidate the role of the NAcc in social behavior of the M. domestica. The present study was designed …

Cover, Title Page, Table Of Contents, University Of Dayton

Research and Reflection on Learning and Teaching in Higher Education

Cover, title page, table of contents, and other information about the journal

Challenges In Genetic Counseling In Hereditary Cancer Syndromes In A Mexican Oncologic Center, Diana Cristina Perez-Ibave, Diana Cristina De Lourdes Perez Ibave, María Fernanda Noriega-Iriondo, Omar Alejandro Zayas-Villanueva, Fernando Alcorta-Nuñez, Juan Francisco González-Guerrero, Adelina Alcorta-Garza, David Hernandez-Barajas, Oscar Vidal-Gutierrez, Carlos Horacio Burciaga-Flores

Research Symposium

Background: In Mexico, hereditary cancer is underdiagnosed, medical geneticists give genetic counseling, but the access is limited due to the socio-economic characteristics of the population. The CUCC (Centro Universitario Contra el Cáncer) Early Cancer Detection Clinic (CECIL) created a model in which patients without cancer are enrolled in a prevention cancer screening program.

Methods: From 2016 to 2021, 3014 patients were enrolled in the prevention program. Patients were evaluated with a hereditary cancer risk survey before a consultation. Those with at least one familial hereditary risk positive answer were assessed in a consultation. We also included patients with cancer diagnoses …

Cast Off Body And Mind: Realization Of The Self In Phenomenology And Soto Zen, Christian Mecham

International Journal of Undergraduate Research and Creative Activities

Throughout much of the history of Western philosophy, the works of non-Western philosophers were often categorized as belonging to that of mystics and was not worthy of rational discourse. This attitude began to change in the mid-19th century with philosophers such as Arthur Schopenhaur, but even more so in the 20th century as exploration of Eastern philosophy began in full earnest. Of particular note is Martin Heidegger’s examination of Buddhism in his phenomenological work. Heidegger is not the only phenomenologist to have connections to Buddhism. Maurice Merleau-Ponty, the leading French phenomenologist of the 20th century, argued that the body was …

Foxo Transcription Factors & Gene Expression, Larissa Barroso, Angelica M. Reyes, David Flores, Millat Jahan, Shania Pintor, Alma Lopez, Megan Keniry

Research Symposium

This paper attempts to highlight ForkHead box transcription factors (FOXO -1, -3, and -4) importance of subcellular localization in U87MG and myoblasts.

Obesity Management In Primary Care Medicine: A Review Of Obesity Perception And Barriers To Weight Loss, Asma Syed, Saima Siddiqui

Research Symposium

Background: This project addresses the obesity epidemic in America. According to the CDC, in 2018, the US obesity prevalence was 42.4%. Obesity is linked to many conditions including heart disease, stroke, type 2 diabetes, and certain types of cancer. Successfully treating obesity can decrease these leading causes of preventable and premature deaths. A proper understanding of the successes and failures of current obesity management in primary care medicine with the help of qualitative research through patient and provider interviews can elucidate the best practices to reduce the burden of disease in America.

Methods: A review of literature was conducted …

Seasonal Dynamics Of Karst Surface Dissolution Based On A Limestone Tables Experiment (Slovak Karst), Alena Gessert, Zdenko Hochmuth

International Journal of Speleology

Chemical denudation in karst is the basic process of karst relief formation. However, it is influenced by many factors of varying intensity that depend on the climatic conditions and characteristics of each given location. Based on measurable results of weight loss of limestone tablets, we monitored the intensity of chemical denudation in two areas of the Slovak Karst, Silica Plateau and Jasov Plateau that are situated in the most developed karst plateau region of Slovakia. Both experimental sites are similar in terms of geographical conditions, thus comparable between them. In each locality, we placed three sets of standardized tablets at …

The First Record Of Orthochirus Glabrifrons (Kraepelin, 1903) (Scorpiones: Buthidae) From The United Arab Emirates, František Kovařík, Ersen A. Yağmur, Alexander Ullrich, Balázs Buzás

Euscorpius

Orthochirus glabrifrons (Kraepelin, 1903) (Scorpiones: Buthidae) was described from Oman (Muscat). Here, we summarize known localities from Oman as well as records from the United Arab Emirates, which is the new country record for this species. Illustrations of morphology of both sexes are given together with a map of distribution. A lectotype of Orthochirus glabrifrons (Kraepelin, 1903) is designated. Paraorthochirus kaspareki Lourenço & Huber, 2000 and Paraorthochirus kinzelbachi Lourenço & Huber, 2000 are synonymized with Orthochirus glabrifrons (Kraepelin, 1903), syn. n.

Dynamic Reconfiguration Of Switchgrass Proteomes In Response To Rust (Puccinia Novopanici) Infection, Nathan A. Palmer, Sophie Alvarez, Michael J. Naldrett, Anthony Muhle, Gautam Sarath, Serge J. Edmé, Satyanarayana Tatineni, Robert B. Mitchell, Gary Yuen

Department of Plant Pathology: Faculty Publications

Switchgrass (Panicum virgatum L.) can be infected by the rust pathogen (Puccinia novopanici) and results in lowering biomass yields and quality. Label-free quantitative proteomics was conducted on leaf extracts harvested from non-infected and infected plants from a susceptible cultivar (Summer) at 7, 11, and 18 days after inoculation (DAI) to follow the progression of disease and evaluate any plant compensatory mechanisms to infection. Some pustules were evident at 7 DAI, and their numbers increased with time. However, fungal DNA loads did not appreciably change over the course of this experiment in the infected plants. In total, 3830 …

Mixed Method Approach Towards The Life Of University Students During The Covid-19 Pandemic, Raihan Khan, Andrew White, Tony Jehi

Department of Health Sciences - Faculty Scholarship

Background

The COVID-19 pandemic negatively impacted the higher education system. This mixed-methods study aimed to assess COVID-19 fear, anxiety, and stress among Shenandoah Valley college students.

Methods

An online survey was fielded and completed by n=680 students. Qualitative semi-structured interviews were conducted with 20 students.

Results

The mean participant age was 22.14±5.48 years, and primarily White (81.9%), women (80.4%), and undergraduate (78.0%) students. Approximately 41% were enrolled in health-related majors (41.4%). Women students had significantly higher anxiety, depression, and fear of contracting COVID-19 than men. Undergraduate students had significantly higher depression than graduate students. Qualitative analysis revealed several major themes: …

Cercocarpus Ledifolius Var. Intricatus ‘Doubledown’, Asmita Paudel, Youping Sun, Larry A. Rupp

Plants, Soils, and Climate Faculty Publications

Bonsai (tray landscape, potted scenery, potted landscape, miniature trees, and rockery) is an artistic horticulture practice of developing aesthetically formed trees and landscapes in miniature with appropriately aesthetic containers. This has been practiced over a few thousand years in oriental cultures, including the ancient Chinese tradition of penzai or penjing, from which the art originated; the miniature living landscapes of Vietnamese hòn non bộ; and the Japanese variations of bonsai and “tray planting” (Gustafson 1995). To produce bonsai plants that share similar shapes and styles of mature, full-size trees, cultivation techniques are used, including leaf trimming, …

Rotunda - Vol 103, No 2 - Sep 27, 2023, Longwood University

Rotunda

No abstract provided.

Uab Biomedical Sciences Ph.D. Student Selected As Inaugural Howard Garrison Advocacy Fellow Under Faseb, Micah Hardge

Browse All News

No abstract provided.

Gardner-Webb College Of Health Sciences Partners With Remote Area Medical To Serve Community, Office Of University Communications

Gardner-Webb NewsCenter Archive

Over 300 volunteers responded to the call from Gardner-Webb University’s College of Health Sciences to partner with Remote Area Medical on Sept. 23-24 to provide free medical and dental care to over 50 people. Seven patients, who had eyeglass prescriptions less than a year old, also received free glasses.

Ms 250 Guide To James "Red" Duke, Jr., Md Papers On Kazakhstan Health Care (1949-2014), James Henry Duke Jr

Manuscript Finding Aids

The collection primarily consists of video recordings, television scripts, and interview transcripts of Dr. Duke’s popular nationally and internationally syndicated television programs, “Dr. Red Duke’s Health Reports”, “Body Watch”, and “Life is Difficult” from 1987-1998. These materials illustrate his valuable contributions as a television host, engaging and educating both the public and the medical community through live medical consultations, surgical procedures, and international teleconferences. See more at MS 250.

Circulating Endocannabinoids And N-Acylethanolamines In Individuals With Cannabis Use Disorder-Preliminary Findings, Nadia Boachie, Erin Gaudette, Richard P. Bazinet, Lin Lin, Rachel F. Tyndale, Esmaeil Mansouri, Marilyn A. Huestis, Junchao Tong, Bernard Le Foll, Stephen J. Kish, Tony P. George, Isabelle Boileau

Institute of Emerging Health Professions Faculty Papers

BACKGROUND: Endocannabinoids and related N-acylethanolamines (NAEs) are bioactive lipids with important physiological functions and putative roles in mental health and addictions. Although chronic cannabis use is associated with endocannabinoid system changes, the status of circulating endocannabinoids and related NAEs in people with cannabis use disorder (CUD) is uncertain.

METHODS: Eleven individuals with CUD and 54 healthy non-cannabis using control participants (HC) provided plasma for measurement by high-performance liquid chromatography-mass spectrometry of endocannabinoids (2-arachidonoylglycerol (2-AG) and N-arachidonoylethanolamine (AEA)) and related NAE fatty acids (N-docosahexaenoylethanolamine (DHEA) and N-oleoylethanolamine (OEA)). Participants were genotyped for the functional gene variant of FAAH (rs324420, C385A) which …

Synlight: A Bicistronic Strategy For Simultaneous Active Zone And Cell Labeling In The Drosophila Nervous System, Michael A. Aimino, Jesse Humenik, Michael J. Parisi, Juan Carlos Duhart, Timothy J. Mosca

Farber Institute for Neuroscience Faculty Papers

At synapses, chemical neurotransmission mediates the exchange of information between neurons, leading to complex movement, behaviors, and stimulus processing. The immense number and variety of neurons within the nervous system make discerning individual neuron populations difficult, necessitating the development of advanced neuronal labeling techniques. In Drosophila, Bruchpilot-Short and mCD8-GFP, which label presynaptic active zones and neuronal membranes, respectively, have been widely used to study synapse development and organization. This labeling is often achieved via the expression of 2 independent constructs by a single binary expression system, but expression can weaken when multiple transgenes are expressed by a single driver. Recent …

Unh's Center Of Integrated Biomedical And Bioengineering Research Receives $10m Nih Grant, Sarah Schaier

UNH Today Archive

No abstract provided.

Archean (3.3 Ga) Paleosols And Paleoenvironments Of Western Australia, Gregory J. Retallack, Mark D. Schmitz

Geosciences Faculty Publications and Presentations

The Pilbara craton of northwestern Australia is known for what were, when reported, the oldest known microfossils and paleosols on Earth. Both interpretations are mired in controversy, and neither remain the oldest known. Both the microfossils and the paleosols have been considered hydrothermal artefacts: carbon films of vents and a large hydrothermal cupola, respectively. This study resampled and analyzed putative paleosols within and below the Strelley Pool Formation (3.3 Ga), at four classic locations: Strelley Pool, Steer Ridge, Trendall Ridge, and Streckfuss, and also at newly discovered outcrops near Marble Bar. The same sequence of sedimentary facies and paleosols was …

Video Based Estimation Of Surface Currents In A Tidal Inlet, Benjamin Middour, George Voulgaris, Douglas Cahl

Faculty Publications

Oceanic surface currents are estimated from analysis of short duration video obtained by a nadir facing camera installed on a bridge over a tidal inlet. Data analyzed consisted of 13 two-minute video clips captured approximately every 40 min over a period of 8 hr, covering a significant portion of the tidal cycle. Surface current speed and direction estimates are obtained from each collected video using (i) a method originally developed for analysis of imagery from drones (CopterCurrents) and (ii) a particle tracking method. The horizontal (u, v) surface current velocity components and total horizontal velocity magnitude, obtained from the two …

Repurposing Normal Chromosomal Microarray Data To Harbor Genetic Insights Into Congenital Heart Disease, Nephi Walton, Hoang Nguyen, Sara Procknow, Darren Johnson, Alexander Anzelmi, Patrick Jay

Department of Medicine Faculty Papers

About 15% of congenital heart disease (CHD) patients have a known pathogenic copy number variant. The majority of their chromosomal microarray (CMA) tests are deemed normal. Diagnostic interpretation typically ignores microdeletions smaller than 100 kb. We hypothesized that unreported microdeletions are enriched for CHD genes. We analyzed "normal" CMAs of 1762 patients who were evaluated at a pediatric referral center, of which 319 (18%) had CHD. Using CMAs from monozygotic twins or replicates from the same individual, we established a size threshold based on probe count for the reproducible detection of small microdeletions. Genes in the microdeletions were sequentially filtered …

Wind Symphony

Bailey School of Music Concert Programs

Program for the Wind Symphony performance on September 27, 2023

Antibodies To Coagulase Of Staphylococcus Aureus Crossreact To Efb And Reveal Different Binding Of Shared Fibrinogen Binding Repeats, Federico Bertoglio, Ya-Ping Ko, Sheila Thomas, Liliana Giordano, Francesca Romana Scommegna, Doris Meier, Saskia Polten, Marlies Becker, Srishtee Arora, Michael Hust, Magnus Höök, Livia Visai

Faculty, Staff and Student Publications

Staphylococcus aureus pathology is caused by a plethora of virulence factors able to combat multiple host defence mechanisms. Fibrinogen (Fg), a critical component in the host coagulation cascade, plays an important role in the pathogenesis of this bacterium, as it is the target of numerous staphylococcal virulence proteins. Amongst its secreted virulence factors, coagulase (Coa) and Extracellular fibrinogen-binding protein (Efb) share common Fg binding motives and have been described to form a Fg shield around staphylococcal cells, thereby allowing efficient bacterial spreading, phagocytosis escape and evasion of host immune system responses. Targeting these proteins with monoclonal antibodies thus represents a …

Exploring Conformational Landscapes And Cryptic Binding Pockets In Distinct Functional States Of The Sars-Cov-2 Omicron Ba.1 And Ba.2 Trimers: Mutation-Induced Modulation Of Protein Dynamics And Network-Guided Prediction Of Variant-Specific Allosteric Binding Sites, Gennady M. Verkhivker, Mohammed Alshahrani, Grace Gupta

Mathematics, Physics, and Computer Science Faculty Articles and Research

A significant body of experimental structures of SARS-CoV-2 spike trimers for the BA.1 and BA.2 variants revealed a considerable plasticity of the spike protein and the emergence of druggable binding pockets. Understanding the interplay of conformational dynamics changes induced by the Omicron variants and the identification of cryptic dynamic binding pockets in the S protein is of paramount importance as exploring broad-spectrum antiviral agents to combat the emerging variants is imperative. In the current study, we explore conformational landscapes and characterize the universe of binding pockets in multiple open and closed functional spike states of the BA.1 and BA.2 Omicron …

Office Of Research, Economic Engagement And Outreach Update, September 27, 2023, Office Of Research, Economic Engagement And Outreach

Office of Research, Economic Engagement and Outreach

No abstract provided.

Detection Of Cancer-Associated Gene Mutations In Urinary Cell-Free Dna Among Prostate Cancer Patients In South Africa, Dada Oluwaseyi Temilola, Martha Wium, Juliano Paccez, Azola Samkele Salukazana, Solomon O. Rotimi, Hasan H. Otu, Giuseppina M. Carbone, Lisa Kaestner, Stefano Cacciatore, Luiz Fernando Zerbini

Department of Electrical and Computer Engineering: Faculty Publications

Prostate cancer (PCa) is the most common cause of cancer death among African men. The presence of tumor-specific variations in cell-free DNA (cfDNA), such as mutations, microsatellite instability, and DNA methylation, has been explored as a source of biomarkers for cancer diagnosis. In this study, we investigated the diagnostic role of cfDNA among South African PCa patients. We performed whole exome sequencing (WES) of urinary cfDNA. We identified a novel panel of 31 significantly deregulated somatic mutated genes between PCa and benign prostatic hyperplasia (BPH). Additionally, we performed whole-genome sequencing (WGS) on matching PCa and normal prostate tissue in an …

Adjunctive Diagnostic Studies Completed Following Detection Of Candidemia In Children: Secondary Analysis Of Observed Practice From A Multicenter Cohort Study Conducted By The Pediatric Fungal Network, Rachel L Wattier, Robert F T Bucayu, Craig L K Boge, Rachael K Ross, Inci Yildirim, Theoklis E Zaoutis, Debra L Palazzi, Surabhi B Vora, Elio Castagnola, Martha Avilés-Robles, Lara Danziger-Isakov, Alison C Tribble, Tanvi S Sharma, Antonio C Arrieta, Gabriela Maron, David M Berman, Dwight E Yin, Lillian Sung, Michael Green, Emmanuel Roilides, Kiran Belani, José Romero, Pere Soler-Palacin, Eduardo López-Medina, Dawn Nolt, Ibrahim Zaid Bin Hussain, William J Muller, Sarmistha B Hauger, Natasha Halasa, Daniel Dulek, Alice Pong, Blanca E Gonzalez, Mark J Abzug, Fabianne Carlesse, Anna R Huppler, Sujatha Rajan, Catherine Aftandilian, Monica I Ardura, Arunaloke Chakrabarti, Benjamin Hanisch, Christine M Salvatore, Lena Klingspor, Elizabeth D Knackstedt, Irja Lutsar, Maria E Santolaya, Sydney Shuster, Sarah K Johnson, William J Steinbach, Brian T Fisher

Faculty, Staff and Students Publications

BACKGROUND: Adjunctive diagnostic studies (aDS) are recommended to identify occult dissemination in patients with candidemia. Patterns of evaluation with aDS across pediatric settings are unknown.

METHODS: Candidemia episodes were included in a secondary analysis of a multicenter comparative effectiveness study that prospectively enrolled participants age 120 days to 17 years with invasive candidiasis (predominantly candidemia) from 2014 to 2017. Ophthalmologic examination (OE), abdominal imaging (AbdImg), echocardiogram, neuroimaging, and lumbar puncture (LP) were performed per clinician discretion. Adjunctive diagnostic studies performance and positive results were determined per episode, within 30 days from candidemia onset. Associations of aDS performance with episode characteristics …