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2023

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Fairy Shrimp (Anostraca) In The Vernal Pools Of Eastern Washington, Megan Garvey Jan 2023

Fairy Shrimp (Anostraca) In The Vernal Pools Of Eastern Washington, Megan Garvey

EWU Masters Thesis Collection

Vernal pools are ephemeral wetlands that retain water annually from winter and spring precipitation and snowmelt but are dry the rest of the year. Though important habitats and sources of freshwater biodiversity, they are little accounted for in wetland conservation and restoration practices. Like much of the world’s wetlands, they have seen a significant decline from anthropogenic impacts and conversion for alternative land use. Pools are also at significant risk due to the impacts of climate change and invasive species. These small temporary water bodies perform vital ecosystem services and are host to rare and endemic species. Anostraca, or fairy …

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The Perfect Storm: Motherhood, Intimate Partner Violence, And Covid-19, Kelly Blauschild Jan 2023

The Perfect Storm: Motherhood, Intimate Partner Violence, And Covid-19, Kelly Blauschild

Electronic Theses and Dissertations, 2020-2023

Intimate partner violence (IPV) is an insidious societal problem with potentially lethal ramifications. At the onset of the COVID-19 pandemic, widespread conditions and virus risk mitigation procedures like social distancing and stay-at-home orders exacerbated factors that contribute to IPV such as poverty and substance use. Though anyone can experience IPV, mothers carried unparalleled burdens in the early weeks of the COVID-19 pandemic such as increasing childcare responsibilities, growing financial difficulties, and worsening mental health, and many of these factors have been linked to experiencing IPV. The growing body of literature indicates a rise in IPV during COVID-19, but little has …

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Reflections On White-Passing Black Identity, Edi Mucka Jan 2023

Reflections On White-Passing Black Identity, Edi Mucka

Electronic Theses and Dissertations, 2020-2023

Now, there are more options than ever for racial identity. Identities such as Black, White, Native, Asian, and Latino have put into contention how past racial boundaries and definitions interact with newer racial color lines. Racial passing is one concept that captures both older and newer forms of identity maintenance. Racial passing refers to when a person classified as a member of a racial group is accepted or perceived as a member of another. In this thesis, I review the literature on racial passing to understand its history and theoretical explanations. Based on the review, I examine a convenience sample …

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Becoming Racially Aware: A Social Process Of (Re)Constructing An Alternative White Identity, Ashley Sobke Jan 2023

Becoming Racially Aware: A Social Process Of (Re)Constructing An Alternative White Identity, Ashley Sobke

Electronic Theses and Dissertations, 2020-2023

Structural and systemic mechanisms reinforce institutionalized racism, whiteness, and white supremacy in the United States. These mechanisms prove adaptable and resilient, shifting and changing to continuously restructure and reinforce the material reality that people experience within a racialized social order. White people's incomprehension of complicity in racism and privilege, along with color-blind ideologies, perpetuate racialized disparities resulting from this social order and render white superiority as "normal" and "universal." This underlies an often internalized sense of normative, white American identity interconnected with racism and white supremacy. In a society where many white Americans remain sheltered from and/or resistant to acknowledging …

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Evolution And Diversification Of The Novel Chlorophyll D-Based Photosynthetic Apparatus, Nikea Jonelle Ulrich Jan 2023

Evolution And Diversification Of The Novel Chlorophyll D-Based Photosynthetic Apparatus, Nikea Jonelle Ulrich

Graduate Student Theses, Dissertations, & Professional Papers

The origin of novel traits is an important evolutionary process that generates biological diversity. However, we rarely know the underlying mechanisms of diversification following the innovation of a novel trait. Over two billion years ago, the innovation of chlorophyll (Chl) abased photosynthesis by cyanobacteria sparked the oxygenation of Earth, setting the stage for the emergence of multicellular life. Chl a was subsequently acquired by diverse algae and plants, and in the process, these organisms diversified their light-harvesting systems to absorb a range of visible wavelengths (400-700 nm) (1). Due to the ancient origins of Chl a, however, it is difficult …

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Impacts Of Climate And Wildfire On Western Larch Regeneration, Spencer T. Vieira Jan 2023

Impacts Of Climate And Wildfire On Western Larch Regeneration, Spencer T. Vieira

Graduate Student Theses, Dissertations, & Professional Papers

Fire plays a critical role in forests of the western United States (US), but as wildfire and climate deviate from historical patterns, increasing fire activity may significantly alter forest ecosystems. To understand the impacts of changing climate and wildfire activity on conifer forests, we studied the impact of wildfire and annual post-fire climate on western larch (Larix occidentalis) regeneration. We destructively sampled 1651 seedlings from 57 sites within 32 fires that burned at moderate or high severity from 2000-2015 in the northwestern US. Using dendrochronological methods, we estimated germination years of seedlings to calculate annual recruitment rates. We …

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The Effects Of Weather On Facilitating The Intensification And Collapse Of Bark Beetle Outbreaks In Ponderosa Pine Forests Of The Northern Rocky Mountains And The Black Hills, Jordan T. Lestina Jan 2023

The Effects Of Weather On Facilitating The Intensification And Collapse Of Bark Beetle Outbreaks In Ponderosa Pine Forests Of The Northern Rocky Mountains And The Black Hills, Jordan T. Lestina

Graduate Student Theses, Dissertations, & Professional Papers

Bark beetles are a diverse family of insect herbivores, of which the most aggressive species can cause significant tree mortality, and are integral components of disturbance regimes in conifer dominated forests of North America. Recent drought events across the western U.S. have been broadly associated with the initiation of multiple bark beetle outbreaks in ponderosa pine forests during the last two decades. Changing weather conditions, such as those observed during the onset of drought, affect outbreak progression through their influence on bark beetle physiology and life history traits and the overall vigor and defensiveness of host trees. This study used …

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Genetic And Environmental Contributions To Wolbachia-Induced Cytoplasmic Incompatibility, Kelley Van Vaerenberghe Jan 2023

Genetic And Environmental Contributions To Wolbachia-Induced Cytoplasmic Incompatibility, Kelley Van Vaerenberghe

Graduate Student Theses, Dissertations, & Professional Papers

Maternally-transmitted Wolbachia infect the cells of most insect species, but their frequencies in host populations vary. While much of their success can be attributed to their ability to manipulate host reproduction, these manipulations are context-dependent, varying due to several biotic and abiotic factors. Wolbachia’s most common manipulation, cytoplasmic incompatibility (CI), is a conditional sterility phenotype where male-female host compatibility depends on their infection status. Specifically, CI occurs when infected males produce modified sperm that cause increased embryonic lethality unless the female carries a similar infection. Rescuing their eggs from CI increases the relative fitness of infected females, promoting Wolbachia spread …

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Modified Tlr7/8 Adjuvanted Liposomes For Improved Intranasal Delivery Of Subunit Vaccines, Elizabeth Brander Lorentz Jan 2023

Modified Tlr7/8 Adjuvanted Liposomes For Improved Intranasal Delivery Of Subunit Vaccines, Elizabeth Brander Lorentz

Graduate Student Theses, Dissertations, & Professional Papers

Intranasal vaccines hold enormous potential for improving vaccine efficacy against respiratory pathogens by activating the mucosal immune system. The urgency for novel approaches in vaccine development is amplified by the recent COVID-19 pandemic. Most intranasal vaccines that were developed are either live-attenuated or inactivated vaccine candidates that demonstrated poor safety profiles. Protein-based subunit vaccines represent a safe and cost-effective alternative, but they are often weak immunogenically. TLR7/8 adjuvants such as INI-4001 have shown promise for enhancing the humoral and cellular immunogenicity of protein-based subunit vaccines. It is theorized that poor immunogenicity of intranasal protein subunit vaccines is due to low …

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Developing A Natural Bio-Sanitizer For The Effective Removal Of Resilient Biofilms From Dairy Separation Membranes, Sheetal Jha Jan 2023

Developing A Natural Bio-Sanitizer For The Effective Removal Of Resilient Biofilms From Dairy Separation Membranes, Sheetal Jha

Electronic Theses and Dissertations

No abstract provided.

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Addressing Soil Carbon Sequestration Response From Multispecies Dairy Forage Systems And Modeling Rangeland Beef Cow Dry Matter Intake Using Precision Enteric Emissions Measurements, Lillian J. Mcfadden Jan 2023

Addressing Soil Carbon Sequestration Response From Multispecies Dairy Forage Systems And Modeling Rangeland Beef Cow Dry Matter Intake Using Precision Enteric Emissions Measurements, Lillian J. Mcfadden

Electronic Theses and Dissertations

Regenerative agriculture is a pressing matter for the dairy industry to address cropland sustainability and carbon sequestration. One regenerative management practice that has been identified to help with row crop sustainability for key metrics like soil organic carbon (SOC) is complex covers. When producers use complex covers one of the main challenges is that it takes time to detect a change in SOC. However, simulation models are a tool that can be used to help determine if a regenerative practice is a strategy that gives the best results (i.e., increased SOC) while aligning with long-term production goals. Therefore, our objectives …

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Diet And Management Strategies To Mitigate Decreased Feed Intake Associated With Terminal Implant Administration In Finishing Beef Steers, Alexandria M. Kelly Jan 2023

Diet And Management Strategies To Mitigate Decreased Feed Intake Associated With Terminal Implant Administration In Finishing Beef Steers, Alexandria M. Kelly

Electronic Theses and Dissertations

Terminal implant administration in beef steers often leads to decreased feed intake, leading to less growth. Our objective was to identify diet and management changes that can mitigate this decrease in feed intake. Single-sourced Angus x Simmental steers (n=27, initial BW= 385.1 ± 30.8 kg) were utilized in a completely randomized trial to assess the effects of animal movement and increased forage inclusion on rumination and feed intake after re-implanting. Steers were initially implanted with Synovex Choice (100 mg trenbolone acetate and 14 mg estradiol benzoate; Zoetis, Parsippany, NJ), then reimplanted 88 d later with Synovex Plus (200 mg trenbolone …

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Indigenous Toxin Affects Cell Viability, While An Artificial Proteolytic Queueing Causes The Upregulation Of Specific Genes, Mohammed Salahuddin Jan 2023

Indigenous Toxin Affects Cell Viability, While An Artificial Proteolytic Queueing Causes The Upregulation Of Specific Genes, Mohammed Salahuddin

Electronic Theses and Dissertations

Studying bacterial physiology is crucial to understand the fundamental mechanisms that govern bacterial growth, survival, and adaptation. This thesis combines two chapters investigating bacterial physiology by studying important cellular processes like bacterial toxin-antitoxin systems and proteolytic pathways. The first chapter involves studying bacterial toxin-antitoxin systems to understand the regulation and function of hypothetical toxin-antitoxin (TA) systems of bacteria and the effects of these TA systems on bacterial growth and survival. The emergence of these genetic modules in bacterial research and the unrevealing of some of their important roles in cell physiology in recent years has drawn much attention in scientific …

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Advanced Analytical Techniques For The Analysis Of Toxic Inhaled Agent Exposure And Pharmacokinetic Investigation, Sharmin Sultana Jan 2023

Advanced Analytical Techniques For The Analysis Of Toxic Inhaled Agent Exposure And Pharmacokinetic Investigation, Sharmin Sultana

Electronic Theses and Dissertations

Toxic inhalation agents (TIAs) (i.e., chlorine (Cl2), cyanide (HCN), etc.) are highly poisonous. However, they have a variety of industrial uses, such as health, agro-food, building, textiles, transport, leisure activities, cosmetics, etc. In addition, TIAs can be used as chemical agents in terrorist events or conventional warfare. The ability to detect TIAs in biological and environmental samples is important for preparing use, determining individual exposure, limiting hazardous exposure, etc. Therefore, developing simple and sensitive analytical methods for TIAs, TIA metabolites, and antidotes for those toxic agents is very important. The focus of this study was chlorine and cyanide. Chlorine is …

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Removal Of Disinfection Byproducts In Drinking Water Using Biological Filtration, Peng Dai Jan 2023

Removal Of Disinfection Byproducts In Drinking Water Using Biological Filtration, Peng Dai

Electronic Theses and Dissertations

Drinking water disinfection is essential to protecting public health from waterborne diseases. However, the reaction of disinfectants with natural organic matter (NOM) can form carcinogenic disinfection byproducts (DBPs). Drinking water treatment facilities employ precursor control and alternative disinfectants to minimize regulated DBPs formation to comply with Environmental Protection Agency (EPA) guidelines. Despite their efficacy, some limitations have been observed during the application, such as increased cost of enhanced coagulation, high demand for sludge disposal, inconsistency in precursor removal, and formation of more toxic unregulated and unknown DBPs. Therefore, it is important to develop more efficient and safe strategies for DBPs …

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Control Of Persistent Environmental Listeria Monocytogenes Using Phenotypic And Genomic Approaches, Bhaswati Chowdhury Jan 2023

Control Of Persistent Environmental Listeria Monocytogenes Using Phenotypic And Genomic Approaches, Bhaswati Chowdhury

Electronic Theses and Dissertations

No abstract provided.

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Assessing How Long-Term Conservation Agricultural Practices Impact Soil Properties And Soil, Water, And Nutrient Dynamics Through Soil Profile, Ajoy Kumar Saha Jan 2023

Assessing How Long-Term Conservation Agricultural Practices Impact Soil Properties And Soil, Water, And Nutrient Dynamics Through Soil Profile, Ajoy Kumar Saha

Electronic Theses and Dissertations

No abstract provided.

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Expanding The Use Of High-Performance Liquid Chromatography For Varietal Identification Or Verification Of Selected Cereal Crops, Johnna Jorgensen Jan 2023

Expanding The Use Of High-Performance Liquid Chromatography For Varietal Identification Or Verification Of Selected Cereal Crops, Johnna Jorgensen

Electronic Theses and Dissertations

High-performance liquid chromatography (HPLC) is a form of analytical chemistry where adsorption is a key factor in which the purpose is to separate, identify and quantify each component in a mixture. It is the optimal separation technique of chemical and biological compounds that are non-volatile including proteins and natural products (i.e., plant extracts). As each sample component interacts differently with the absorbent material within the column, the flow rates change leading to the separation of peaks, which are plotted via computer software. Most commonly HPLC analysis has been used for chemical manufacturing and pharmaceutical purposes. When using HPLC for crop …

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Identification Of Genes Expressed In The Anterior Pituitary Associated With Water Restriction In Beef Cattle, Himali Chathurika Wickramasinghe Vithana Arachchilage Jan 2023

Identification Of Genes Expressed In The Anterior Pituitary Associated With Water Restriction In Beef Cattle, Himali Chathurika Wickramasinghe Vithana Arachchilage

Electronic Theses and Dissertations

Water is an essential nutrient and is required for growth, metabolism, lactation, and reproduction. Without water, livestock production would not be possible. Despite its importance, water requirements in livestock have not received much attention from the research community since the 1950s. The most recent edition of the Nutrient Requirements of Beef Cattle (8th edition) relies on literature from the 1950s or earlier for defining water requirements in beef cattle. Since the time these requirements were estimated, much has changed with regards to body size, genetics, and productivity of our livestock herds, including beef cattle. Global surface temperatures have increased in …

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Genomics-Assisted Approaches To Improve Grain Yield And End-Use Quality In Hard Winter Wheat (Triticum Aestivum L.), Harsimardeep Singh Gill Jan 2023

Genomics-Assisted Approaches To Improve Grain Yield And End-Use Quality In Hard Winter Wheat (Triticum Aestivum L.), Harsimardeep Singh Gill

Electronic Theses and Dissertations

Global wheat production needs to be increased by 60% to meet the future demand of feeding nine billion people by 2050. Simultaneously, it is important to improve the enduse quality to meet the requirements of producers, grain markets, processors, and consumers. Thus, the development of more productive wheat varieties with better enduse quality remains the primary focus for all wheat breeding programs. However, direct phenotypic selection for improving grain yield and end-use quality is difficult as it is highly influenced by environmental factors. This dissertation focuses on harnessing advancements in genomics applications, including genome-wide association studies (GWAS), for the genetic …

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Simulating The Impact Of Emissions Control On Economic Productivity Using Particle Systems And Puff Dispersion Model, Najam Khan Jan 2023

Simulating The Impact Of Emissions Control On Economic Productivity Using Particle Systems And Puff Dispersion Model, Najam Khan

Electronic Theses and Dissertations

A simulation platform is developed for quantifying the change in productivity of an economy under passive and active emission control mechanisms. The program uses object-oriented programming to code a collection of objects resembling typical stakeholders in an economy. These objects include firms, markets, transportation hubs, and boids which are distributed over a 2D surface. Firms are connected using a modified Prim’s Minimum spanning tree algorithm, followed by implementation of an all-pair shortest path Floyd Warshall algorithm for navigation purposes. Firms use a non-linear production function for transformation of land, labor, and capital inputs to finished product. A GA-Vehicle Routing Problem …

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The Role Of Cadherins And Ryk On Metastasis And Invasion In Triple Negative Breast Cancer Cell Model: Hs578t/Hs578ts(I)8, Ibis Iser Jan 2023

The Role Of Cadherins And Ryk On Metastasis And Invasion In Triple Negative Breast Cancer Cell Model: Hs578t/Hs578ts(I)8, Ibis Iser

Electronic Theses and Dissertations

Breast cancer is a major cause of death among women in European and North American countries, even with improved methods for diagnosis and therapy. The mortality of breast cancer is mainly due to the migration of the primary tumor to distinct sites in the body and is very common in triple negative breast cancer (TNBC). This type of breast cancer affects younger woman and has a high recurrence rate. Unfortunately, TNBC is extremely difficult to control because of the absence of specific targets for treatment. Therefore, our research aim is to discover new therapeutic targets and identify novel approaches for …

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Application Of Crispr/Cas9 Whole Genome Screens To Advance Macrophage Biology, Kevin Wanniarachchi Jan 2023

Application Of Crispr/Cas9 Whole Genome Screens To Advance Macrophage Biology, Kevin Wanniarachchi

Electronic Theses and Dissertations

Advances in CRISPR/Cas9 gene editing technologies present opportunities to better understand the contribution of individual genes to complex eukaryotic cellular processes. I have applied CRISPR whole genome screen technologies to inform further mechanistic understanding of macrophage fitness and neutral lipid metabolism. This work is important because macrophages play critical roles in immune function and are implicated as causative agents in disease states. Chapter One of this dissertation provides insights into how CRISPR genome screening technologies have improved biological discovery and key findings from their application and discusses how this technology can be implemented to advance the discovery of novel macrophage …

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Strategies To Optimize Confinement Fed Beef Cattle In The Northern Plains, Erin R. Gubbels Jan 2023

Strategies To Optimize Confinement Fed Beef Cattle In The Northern Plains, Erin R. Gubbels

Electronic Theses and Dissertations

The goal of this dissertation was to understand the strategies needed to optimize performance of confinement fed beef cattle in the Northern Plains. This was accomplished through three objectives: 1) evaluate health, growth performance, and antibody titers in previously vaccinated, newly weaned calves administered a respiratory and clostridia vaccine upon arrival compared to no vaccination; 2) determine the influence of manger space restriction on program-fed feedlot heifers during the growing phase; and 3) to evaluate growth performance and carcass traits following transit of feedlot heifers sourced and finished in different regions of the United States. For objective 1, single-sourced, newly …

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Soil Profile Properties And Greenhouse Gas Emissions As Influenced By Long-Term Cattle Manure And Inorganic Fertilizer Applications Under Corn-Soybean-Spring Wheat Rotation In Eastern South Dakota, Anuoluwa Ojonoka Sangotayo Jan 2023

Soil Profile Properties And Greenhouse Gas Emissions As Influenced By Long-Term Cattle Manure And Inorganic Fertilizer Applications Under Corn-Soybean-Spring Wheat Rotation In Eastern South Dakota, Anuoluwa Ojonoka Sangotayo

Electronic Theses and Dissertations

The application of manure and inorganic fertilizer in row crops may significantly influence soil and greenhouse gas (GHG) emissions. Understanding the long-term influence of these management practices on soil pore characteristics, hydro-physical properties and greenhouse gas emission is essential in developing proper conservation practices. However, there is limited information on the impact of cattle manure and inorganic fertilizer application on soil hydro-physical properties, soil pore characteristics at lower depths and surface GHGs emissions. Therefore, the objectives of this study were to; (i) utilize X-ray computed tomography (XCT) technique to quantify the impact of manure and fertilizer amendments under a corn …

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Walleye Feeding, Movements, And Energetics Reveal The Importance Of Connected Wetlands In Glacial Lakes, Logan M. Cutler Jan 2023

Walleye Feeding, Movements, And Energetics Reveal The Importance Of Connected Wetlands In Glacial Lakes, Logan M. Cutler

Electronic Theses and Dissertations

Fish require a variety of resources including prey, thermal refugia, and different physical habitat characteristics to grow and reproduce. All necessary resources are rarely provided by a single habitat, resulting in tradeoffs between occupying different habitats; therefore, the ability to move among heterogenous habitats is critical. These tradeoffs may occur in lakes with connected wetlands where the main lake habitats tend to be more thermally stable, but wetlands often provide more diverse and abundant prey. For my second chapter, I compared prey diversity and abundance as well as Walleye (Sander vitreus) diets and condition in a large glacial lake (Lake …

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Ecological Impacts Of Anthropogenic Chemicals On Non-Target Species And The Role Of Plant Diversity In Ecosystem Restoration And Resilience, Shiva Torabian Jan 2023

Ecological Impacts Of Anthropogenic Chemicals On Non-Target Species And The Role Of Plant Diversity In Ecosystem Restoration And Resilience, Shiva Torabian

Electronic Theses and Dissertations

Understanding ecosystem processes in rangelands is of paramount importance for ensuring global food security, given their pivotal role in sustaining forage and livestock production. Central to this understanding are fundamental processes such as nutrient cycling, decomposition, and plant biomass dynamics. However, the use of anthropogenic chemicals in agricultural practices has the potential to exert significant influence on these critical ecosystem functions. A pivotal factor in this context is the plant diversity inherent to these rangeland ecosystems. Plant diversity not only provides essential ecosystem functions but also enhances them with the capacity to withstand and recover from perturbations. Ecosystems characterized by …

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Enhancing Nitrogen Use Efficiency Through Ai-Powered Image Analysis And Innovative N-Rich Spot Method, Bobby Azad Jan 2023

Enhancing Nitrogen Use Efficiency Through Ai-Powered Image Analysis And Innovative N-Rich Spot Method, Bobby Azad

Electronic Theses and Dissertations

This study conducted in 2023 aimed to enhance nitrogen use efficiency (NUE) in wheat and corn grown in South Dakota. Based on dynamic weather conditions and other factor interactions, conventional nitrogen (N) recommendations need to be improved. Soil properties information, including electrical conductivity, was used to create management zones. In each zone, three N-rich spots were established as biosensors. Drones and satellites collected imagery data, and an AI-driven approach assessed the crop response to applied N. A dynamic N application approach, integrating aerial data with historical records, was developed and evaluated. Our methodology, at a 95% confidence level, resulted in …

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Characterizing Vaginal Gene Expression In Prepubertal Gilts, Shannon Lee Dierking Jan 2023

Characterizing Vaginal Gene Expression In Prepubertal Gilts, Shannon Lee Dierking

Electronic Theses and Dissertations

The best-known predictor of reproductive success in gilts is the age at puberty. Early puberty (i.e., d170 – d180 of age) is associated with improved long-term reproductive performance and more full value offspring for market. The ability to predict, in the prepubertal stage, the age that a replacement gilt would be expected to achieve puberty has great reproductive and economic advantages. This work focuses on identifying biological markers that are potentially predictive of age at puberty. Study 1 identified changes in the vaginal epithelium during gilt reproductive development. Pre-pubertal gilts (n =13) were followed from d70 of age until first …

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Investigating Dormancy And Germination Characteristics To Promote Restoration Success In The Northern Great Plains, Gregory A. Cooper Jan 2023

Investigating Dormancy And Germination Characteristics To Promote Restoration Success In The Northern Great Plains, Gregory A. Cooper

Electronic Theses and Dissertations

Failures in seed-based restoration can be partially attributed to a lack of knowledge on seed dormancy. Dormancy is beneficial for plant establishment in unpredictable environmental conditions, but a lack of uniform germination can hinder restoration efforts. The purpose of this research was to gain a better understanding of dormancy displayed by select forbs of the northern Great Plains. The first data chapter attempted to identify and break dormancy class. Seeds were treated with scarification, smoke, and fertilizer pretreatments in an attempt to break seed dormancy. Seeds were also placed in spring and summer temperatures to identify how seasonal phenology influenced …

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