Digital Commons Network^™

Characterizing The Developmental Role Of Autism-Risk Gene Adnp In A Zebrafish Model For Autism, Vaishnavi Balaji

Master's Theses

Autism spectrum disorder is a complex neurodevelopmental disorder characterized by impaired social

skills, restricted/ repetitive behaviors, and developmental delay. The disease has a genetic component,

and mutations in the adnp gene have been found in 0.17% of non-inherited genetic cases of the disorder. The exact molecular mechanism by which adnp is involved in the development of autism is not known. This study aimed to use zebrafish to determine the phenotypic effects of adnp mutations using two adnp paralogs- adnpA and adnpB to understand the underlying mechanism. Novel zygotic adnp double heterozygous mutants (A+/- B+/-) were previously generated using …

“The Cruelty Is The Point”: Using Buck V. Bell As A Tool For Diversifying Instruction In The Law School Classroom, Tiffany C. Graham

Roger Williams University Law Review

No abstract provided.

Structural Files For The Etr1 Ethylene-Receptor Dimer Based On Computational Modeling, Beenish J. Azhar, Safdar Abbas, Sitwat Aman, Maria V. Yamburenko, Wei Chen, Lena Muller, Buket Uzun, David A. Jewell, Jian Dong, Samina N. Shakeel, Georg Groth, Brad M. Binder, Gevorg Grigoryan, G. Eric Schaller

Dartmouth Scholarship

Structural models for the ETR1 homodimer were generated with AlphaFold-Multimer. Coppers were modeled under two potential coordinations involving Cys65 and His69 of the ETR1 homodimer, one in which the two coppers are bound independently and do not share an interaction with each other, and another where they are closely bonded.

See the following publication for details: Azhar, B.J., Abbas, S., Aman, S., Yamburenko, M.V., Chen, W., Müller, L., Uzun, B., Jewell, D.A., Dong, J., Shakeel, S.N., Groth, G., Binder, B.M., Grigoryan, G., Schaller, G.E. (2023) Basis for high-affinity ethylene binding by the ethylene receptor ETR1 of Arabidopsis. Proc. Natl. Acad. …

Modified Periodic Acid-Schiff (Pas) Is An Alternative To Safranin O For Discriminating Bone–Cartilage Interfaces, Kelsey M. Kjosness, Philip Reno, Maria A. Serrat

PCOM Scholarly Works

Cartilage histomorphometry is often performed on decalcified, paraffin-embedded bone sections, which provide versatility in staining applications from basic morphology to immunohistochemistry. Safranin O is a cationic dye that binds to proteoglycans in cartilage and is routinely used to assess growth plate dynamics and/or fracture repair at bone–cartilage interfaces. When used with a counterstain such as fast green, safranin O can offer exquisite differentiation of cartilage from surrounding bone. However, various decalcification and processing methods can deplete proteoglycans, rendering inconsistent, weak, or absent safranin O staining with indiscriminate bone–cartilage boundaries. We sought to develop an alternative staining methodology that preserves the …

Psy 390 Evolutionary Psychology, Dan Mccloskey

Open Educational Resources

No abstract provided.

Environmental Monitoring Plan: For Work To Be Performed: July 1, 2022, Through June 30, 2023, Tennessee. Department Of Environment And Conservation.

Environmental Monitoring Plans

The Tennessee Department of Enviornment and Conservation, Division of Remediation, Oak Ridge (TDEC DoR-OR), provides the annual Fiscal Year 2023 EMP for the period of July 2, 2022, through June 30, 2023. This report is submitted as a comprehensive plan for TDEC DoR-OR monitoring and assessment activities across the Oak Ridge Reservation, Oak Ridge, Tennessee, (ORR) in accordance with terms of both the Environmental Surveillance and Oversight Agreement (ESOA), as well as in support of activities being conducted under the Federal Facilities Agreement (FFA).

Effects Of Growth Factor Deprivation On Mkn-45 Spheroid Cells, Özlem Türksoy, Gökhan Terzi̇oğlu

Turkish Journal of Biology

Background/aim: Serum and growth factor deprivation, a common cellular stressor in solid tumors, arises upon irradiation, chemotherapy, and antiangiogenesis. Spheroid body culture aims to enrich cancer stem cells by using low attachment conditions and some growth factors, such as basic fibroblast growth factor and epidermal growth factor to support the spheroid formation in serum-free spheroid culture. However, spheroid culture without any growth factors can imitate the tumor environment more realistically. In this study, we aimed to identify the effect of growth factor deprivation on the MKN-45 gastric cancer cell line in terms of stemness characteristics. Materials and methods: The spheroids …

Vitamin D Receptor Mediates Liver Ischemia And Reperfusion Injury By Autophagy-Regulated M2 Macrophage Polarization, Mingming Fang, Chen Zhong

Turkish Journal of Biology

Liver ischemia and reperfusion (IR) injury is the major complication of liver-related operations. Macrophage polarization has an essential effect on the mechanism of liver IR injury. Vitamin D receptor (VDR) has been found to regulate macrophage polarization and alleviate IR injury. Nevertheless, the correlation between VDR and macrophage polarization in liver IR injury has not been clearly elucidated. VDR knockout mice and wild-type littermates underwent partial liver ischemia for 90 min and reperfusion for 6 h. RAW264.7 cells were also used to verify the influence of VDR on macrophage polarization in vitro. VDR activation could promote M2 macrophage polarization and …

Antiadipogenic And Antiobesogenic Effects Of Pterostilbene In 3t3-L1 Preadipocyte Models, Bi̇rgül Gülnar, İpek Canatar, Si̇bel Özdaş

Turkish Journal of Biology

Since obesity causes at least 2.8 million death each year and is a major risk factor for many diseases, it is critical to evaluate alternative treatment approaches. In this context, studies on the research of natural product-based therapeutics in the fight against obesity are increasing. In this study, it was aimed to evaluate the antiadipogenic and antiobesogenic efficacy of pterostilbene a natural phenolic compound in 3T3-L1 cells. The mature 3T3-L1 adipocytes were exposed to pterostilbene at different concentrations and half-maximum inhibitory concentrations (IC50) were determined by MTT assay. Oil-Red-O staining was applied to determine lipid accumulation. Phase contrast microscopy, Giemsa, …

Cover And Contents

Turkish Journal of Botany

No abstract provided.

Distributional Ecology Of Noccaea Vesicaria In Relation To The Anatolian Diagonal, İlayda Dumlupinar, Hakan Gür, Bariş Özüdoğru

Turkish Journal of Botany

Anatolia is located at the intersection of three of the world's 36 biodiversity hotspots (the Caucasus, Irano-Anatolian, and Mediterranean Basin biodiversity hotspots) and has about 10,000 plant species, over 3000 of which are endemic. Among the many reasons for the astonishing biodiversity of Anatolia, one that stands out is the Anatolian Diagonal. Although the Anatolian Diagonal has long been recognized as a biogeographical boundary between Central and Eastern Anatolia, its exact functioning is still not well understood. Thus, in this study, our aims were 1) to assess the environmental barrier function of the Anatolian Diagonal for a plant species, Noccaea …

Haplotype Diversity And Molecular Phylogeny Of Wild Crambe L. (Brassicaceae) Taxa Of Turkey, Burcu Tarikahya Hacioğlu, Kurtuluş Özgi̇şi̇

Turkish Journal of Botany

Revealing genetic diversity is both essential for plant systematics and also provides important information for agricultural sciences. Crambe hispanica var. abyssinica is an oil seed crop. Wild plants related to crops (Crop Wild Relatives) are important resources for the genetic improvement of cultivated species. In order to reveal the genetic diversity of wild Crambe taxa of Turkey, we used ITS and trnL-F markers to create the haplotype networks and phylogeny reconstruction. Thirty-nine accessions belonging to 8 Crambe taxa were used as the material of our study: Crambe orientalis var. orientalis with 18 accessions, Crambe orientalis var. sulphurea with 3 accessions, …

Seed Morphology Of Some Taxa Of The Genus Euphorbia L. (Euphorbiaceae) In Turkey And Its Taxonomic Significance, Murat Kurşat, Bi̇rol Başer, Fahretti̇n Özbey, İrfan Emre

Turkish Journal of Botany

The morphological characteristics of seeds are not affected by environmental factors and can be used to identify the Euphorbia taxa. The present study aims to determine the morphological characteristics of seeds in 27 taxa of Euphorbia using scanning electron microscopy (SEM) and light microscope (LM). Findings revealed that the seeds of Euphorbia show variation in size, color, and seed surface ornamentations among different species. The seeds vary also in shape from ovoid, ellipsoidal, globose, quadrangular, compressed, to compressed-ellipsoidal with two exceptions in E. petiolata (oblong--quadrangular truncate), E. macrocarpa (subglobose), and E. szovitsii var. kharputensis(ellipsoidal quadrangular truncate). Furthermore, it was …

Early Pliocene Arvicolinae And Cricetinae From The Locality Of Afşar, Western Turkey, Panagiotis Skandalos, Koen Lansing, Fatma Arzu Demi̇rel, Mehmet Ci̇hat Alçi̇çek, Serdar Mayda, Francien Dieleman, Lars W. Van Den Hoek Ostende

Turkish Journal of Earth Sciences

The Afşar section, situated in the Dombayova graben in western Turkey, is one of the key localities for the study of the Pliocene of Anatolia. Two fossiliferous layers yielded micromammal assemblages, including various cricetine and arvicoline species. These include the species Mimomys cf. gracilis, Pliomys sp., Arvicolinae gen. sp. and the cricetines Cricetulus cf. ehiki and Cricetulus sp. in Afşar 1 and Mimomys hassiacus, M. gracilis, Pliomys graecus and Mesocricetus primitivus in Afşar 2. The cooccurence of these species indicates a dry and open spaced habitat. Based on the composition and stage of evolution of the hamster and vole species, …

The Next 100 Years Of International Intellectual Property: Integrating Human Rights And Corporate Social Responsibility, J. Janewa Osei-Tutu

Articles

No abstract provided.

Stage-Structured Predator-Prey Interactions In A Warming World: A Case Study In Riverine Rock Pools, Andrew T. Davidson

Theses and Dissertations

Warming can impact predator-prey interactions through a variety of mechanisms. For example, warming can both alter the rate at which predators consume prey and the rate prey develop through vulnerable life stages. Thus, the overall effect of warming on consumer-resource interactions will depend upon the strength and asymmetry of warming effects on predator and prey performance. Here, we describe the consequences of these asymmetrical responses to temperature by first developing a simple stage-structured modeling framework, then applying that framework to predation of American rock pool mosquito larvae, Aedes atropalpus, by several common predators in riverine rock pools. We then …

Interactions Between Hiv And Opioids On Antiretroviral Accumulation, The Blood Brain Barrier, And The Inflammatory Response In The Brain., Kara Rademeyer

Theses and Dissertations

The complex mechanisms related to HIV infection, neurodegeneration, and chronic neuroinflammation collectively describe neuroHIV (Hauser et al. 2007; Chang et al. 2014; Smith et al. 2014). Specifically, opioid abuse, poor penetration of antiretroviral (ARV) drugs, chronic inflammation and neuronal injury/degeneration are all implicated in neuroHIV (Fantuzzi et al. 2003; Letendre et al. 2004; Verani et al. 2005; Duncan and Sattentau 2011; Hong and Banks 2015; Simoes and Justino 2015; Olivier et al. 2018; Murphy et al. 2019; Osborne et al. 2020). For the first time, we demonstrate that morphine, fentanyl, and methadone in vivo alter the brain accumulation of ARVs, …

Optimizing Crispr/Cas9 Transfection Protocol To Generate A Tet-On Inducible Sk-Mel 24 Cell Lines To Better Study Icer, Abdulkader Hallak

Theses, Dissertations and Culminating Projects

Inducible cAMP Early Repressor (ICER) is a dominant transcription repressor of the cAMP Responsive Element Modulator (CREM) and cAMP Responsive Element Protein (CREB) family of transcription factors. ICER isoforms are differently expressed in various cell types and are involved in essential functions including hormonal regulation, circadian rhythm, spermatogenesis, and immune response. In the context of cancer, ICER is proposed as a tumor suppressor in leukemia, prostate, pituitary cancers, and melanoma. In melanoma, ICER is targeted for degradation during tumorigenesis and highly upregulated during regression, further highlighting its potency as a tumor suppressor. Inside the nucleus, ICER targets oncogenes and key …

Secondary Analysis Of Chip-Seq Dataset And Genomic Interactions Of Icer, Karem Rivera

Theses, Dissertations and Culminating Projects

The Inducible cyclic AMP (cAMP) Early Repressor (ICER) is an endogenous transcriptional repressor of the cAMP-mediated signaling gene transcription pathway and belongs to the CRE-binding protein (CREB)/CRE modulator (CREM) gene family. ICER is associated to many functions and biological processes in the immune system, brain, regulation of spermatogenesis, circadian control of transcription, apoptosis and metabolic functions, and of particular interest, has been shown to act like a tumor suppressor. Recent findings from our laboratory suggest that ICER might have a role in DNA repair, but further experiments need to be performed. In this research, we aimed to elucidate the function …

Design Of Block Copolymer With Tunable Hydrophobic/Hydrophilic/Fluorophilic Interactions, Tatiane De Fatima Dutra

Theses, Dissertations and Culminating Projects

Block copolymers (BCP) with balanced interactions and their resultant bulk-phase self-assembly have become increasingly important in advancing nanotechnology, separation, and energy applications. However, a few reports have addressed the synthesis challenge and bulk-phase self-assembly of such triblock copolymers. This thesis presents a facile route for preparing triblock copolymer via controlled radical and organocatalytic ring-opening polymerization that allows precise control over the incorporation of individual moieties in resultant polymer, responsible for balanced hydrophilic, hydrophobic/lipophilic, and fluorophilic interactions. Synthesized polymer with suggested self-assembled 2D lamellar nanostructure exhibits high-temperature stability. The long-term goal of this work is to selectively use this polymer class …

A Computational Study Of The Copper-Catalyzed Trifluoromethylation Of Boronic Acids, Kevin Nyhuis

Theses, Dissertations and Culminating Projects

This work is a computational study of a reaction mechanism for the trifluoromethylation of boronic acids. Three steps of the proposed reaction mechanism are studied, •CF₃ addition to a copper catalyst center, base promoted transmetalation between copper and aryl boronic acid, and bond forming reductive elimination in which the CF₃ and aryl substituent form a bond. Limited information is known about this mechanism. This study uses computational methods to attempt to elucidate the mechanism and provide the groundwork for potential improvement. Quantum chemical methods in conjunction with tight-binding based conformational sampling methods are used to investigate the possible …

Investigating The Roles Of Active Site Residues K59, K119 And E168 In M. Tuberculosis Indole-3-Glycerol Phosphate Synthase, Maryum M. Bhatti

Theses, Dissertations and Culminating Projects

Tuberculosis (TB) is a disease that is caused by a bacteria called Mycobacterium tuberculosis and primarily attacks the lungs but can spread to other areas of the body as well. TB can develop resistance to drugs that may be used to cure the disease. Multidrug resistant TB is especially a growing issue as it does not respond to the two most powerful anti-TB drugs, isoniazid and rifampicin. For this reason, finding new ways to target TB are critically needed. One possible way to do this is to target the enzymes in the tryptophan biosynthetic pathway of M. tuberculosis. Studies have …

Future Time Orientation And Criminal Thinking Style, Danielle Squillaro

Theses, Dissertations and Culminating Projects

Individuals who commit criminal behaviors are often thought to prioritize short-term goals rather than long-term goals (i.e., a present vs. a future time orientation). Though previous theories of crime and empirical research support a relationship among future time orientation, criminal thinking, and illegal behaviors, there is disagreement in the literature about how to operationalize future time orientation. Moreover, prior research has usually only included a single measure of future time orientation, making generalizability of the results across different measures (reflecting different operationalizations of the construct) difficult. The primary aim of the current thesis was to measure multiple components of future …

Destruction Of Pfoa On Ion-Exchange Resin With Advanced Reduction Processes To Regenerate Resins For Addressing Pfoa Pollution In Drinking Water, Junkui Cui

Theses, Dissertations and Culminating Projects

Per- and polyfluoroalkyl substances (PFAS) have been globally incorporated into various industrial and consumer products since the 1940s. However, concerns about PFAS have gradually grown because of their prevalence, mobility, persistence, bioaccumulation, and adverse impacts on human and environmental health. Unfortunately, traditional water treatment processes inefficiently remove PFAS. Therefore, there is an urgent research need to develop innovative, technically viable, and low cost-treatment processes for the removal of PFAS in water.

Among the established PFAS treatment technologies, ion-exchange (IX) has been extensively applied to drinking water treatment practices due to its adsorption capability and technology maturity. However, IX is highly …

Contextualizing The Guilty Plea Experiences Of Individuals Charged With Sex Crimes, Conor Hogan

Theses, Dissertations and Culminating Projects

Little research has been done on the guilty plea experiences of individuals charged with sex crimes. Persons convicted of sex crimes face unique legal and extralegal consequences, and often experience social stigma. I interviewed 60 males charged with sex crimes who have accepted a guilty plea on their decision making. I report contextual data of their guilty plea experiences, as well as their rationales for pleading guilty, including whether concerns over stigma and collateral consequences influenced their decision making. For most participants, primary rationales for pleading guilty were reduced time or charges, consistent with the most common reasons given by …

Road Salt-Induced Salinization Of The Upper Paulins Kill River In Newton, New Jersey, Kristine Emily Rogers

Theses, Dissertations and Culminating Projects

Road salt application is a primary factor leading to the salinization of freshwater rivers and streams throughout the Northeastern United States and globally. Rising salinization within fresh water bodies is problematic because it can modify community structure and detritus processing within freshwater ecosystems, induce mortality among macroinvertebrates and other aquatic life, and mobilize metals that can pose harm to human health. Road salts also threaten surface and underground drinking water supplies, kill riparian vegetation, and corrode infrastructure, such as bridges and roads. This research examines 4 1⁄2 years of continuous water quality monitoring data collected from two sensor stations along …

Methods Of Teaching Early Literacy, Nandita Gurjar, Sohyun Meacham, Constance Beecher

Faculty Book Gallery

Theories, teaching strategies, and instructional materials pertinent to teaching reading and writing in grades PK-3, with an emphasis on integrating reading, writing, speaking, and listening, as well as integration across content areas while addressing diversity and inclusion. -- Provided by the publisher

Privatizing Family Leave Policy: Assessing The New Opt-In Insurance Model, Deborah Widiss

Articles by Maurer Faculty

Federal law fails to guarantee new parents or family caregivers paid time off from work. A growing number of blue-leaning states have addressed this gap by enacting comprehensive paid family and medical leave laws, typically funded by a small payroll tax. A new—and quite different—approach is expanding rapidly in red-leaning states: authorization of commercial “Family Leave Insurance” to be marketed to employers. In other words, this is an opt-in privatized approach to family leave policy.

This Article, written for a symposium held by the Seton Hall Law Review, offers the first analysis in the legal literature of opt-in Family Leave …

Pronostic Of Colo-Rectal Cancer (Crc) Using Machine Learning Models On Organoids Derived Of Patient, Claudia Andrea Leiva Acevedo

ICT

Colorectal Cancer (CRC) is a globally prevalent and deadly carcinoma, necessitating advanced treatment approaches. Despite ongoing advancements, the mortality rate remains high. Various biological models, including animal studies, cell lines, and the emerging organoid model, contribute to understanding molecular mechanisms. Organoids, 3D cultures derived from tumor epithelial cells, offer advantages such as enhanced diversity, genetic modification, and extended culture capabilities. Recent applications of machine learning (ML) in predicting CRC treatment responses using organoids and tissue data indicate a promising avenue for advancing personalized therapies.

Shifting The Male Gaze Of Evidence, Teneille R. Brown

Utah Law Faculty Scholarship

In this article I target the altar at which many of us worship—the pursuit of rationality. For evidence purposes, rationality is defined as decisions that are reasonable, objective, inductive, and free from the bias of emotion. This view of rationality is deeply embedded in evidence scholarship and practice. It is also reflected in evidence rules like FRE 403, which treat emotional testimony as unfairly prejudicial simply because it is emotional. The anti-emotion view of rationality reflects the thinking of Western philosophical giants. Plato, Hobbes, Descartes, and Bacon all thought that men should strive for rationality by suppressing their emotions, because …