Digital Commons Network^™

Fresh Produce As A Potential Vector And Reservoir For Human Bacterial Pathogens: Revealing The Ambiguity Of Interaction And Transmission, Ahmed Esmael, Rashad R. Al-Hindi, Raed S. Albiheyri, Mona G. Alharbi, Amani A. R. Filimban, Mazen S. Alseghayer, Abdulaziz M. Almaneea, Meshari Ahmed Alhadlaq, Jumaa Ayubu, Addisu D. Teklemariam

Nebraska Center for Virology: Faculty Publications

The consumer demand for fresh produce (vegetables and fruits) has considerably increased since the 1980s for more nutritious foods and healthier life practices, particularly in developed countries. Currently, several foodborne outbreaks have been linked to fresh produce. The global rise in fresh produce associated with human infections may be due to the use of wastewater or any contaminated water for the cultivation of fruits and vegetables, the firm attachment of the foodborne pathogens on the plant surface, and the internalization of these agents deep inside the tissue of the plant, poor disinfection practices and human consumption of raw fresh produce. …

Epidemic Highs And Lows: A Stochastic Diffusion Model For Active Cases, Luis F. Gordillo, Priscilla E. Greenwood, Dana Strong

Mathematics and Statistics Faculty Publications

We derive a stochastic epidemic model for the evolving density of infective individuals in a large population. Data shows main features of a typical epidemic consist of low periods interspersed without breaks of various intensities and duration. In our stochastic differential model, a novel reproductive term combines a factor expressing the recent notion of ‘attenuated Allee effect’ and a capacity factor is controlling the size of the process. Simulation of this model produces sample paths of the stochastic density of infectives, which behave much like long-time Covid-19 case data of recent years. Writing the process as a stochastic diffusion allows …

Increased Hepatic Interleukin-1, Arachidonic Acid, And Reactive Oxygen Species Mediate The Protective Potential Of Peptides Shared By Gut Cysteine Peptidases Against Schistosoma Mansoni Infection In Mice, Hatem Tallima, Rashika El Ridi

Faculty Journal Articles

No abstract provided.

The Long Term Impact Of Covid-19 On Nursing: An E-Panel Discussion From The International Network For Child And Family Centred Care, Mandie Jane Foster, Julie Blamires, Sarah Neill, Imelda Coyne, G Kristjansdottir, Veronica D. Feeg, Ann Marie Paraszczuk, Mohammad Al-Motlaq

School of Nursing and Midwifery

No abstract provided.

An Essential Endoplasmic Reticulum-Resident N-Acetyltransferase Ortholog In Plasmodium Falciparum, Alexander J Polino, Muhammad M Hasan, Katherine Floyd, Yolotzin Avila-Cruz, Yujuan Yang, Daniel E Goldberg

2020-Current year OA Pubs

N-terminal acetylation is a common eukaryotic protein modification that involves the addition of an acetyl group to the N-terminus of a polypeptide. This modification is largely performed by cytosolic N-terminal acetyltransferases (NATs). Most associate with the ribosome, acetylating nascent polypeptides co-translationally. In the malaria parasite Plasmodium falciparum, exported effectors are thought to be translated into the endoplasmic reticulum (ER), processed by the aspartic protease plasmepsin V and then N-acetylated, despite having no clear access to cytosolic NATs. Here, we used inducible gene deletion and post-transcriptional knockdown to investigate the primary ER-resident NAT candidate, Pf3D7_1437000. We found that it localizes to …

Diatom Diversity And Functional Groups In 72 Florida Lakes: Assessing Ecological Changes For Improved Protection And Management, Francesca M. Lauterman

USF Tampa Graduate Theses and Dissertations

Florida lakes are diverse ecosystems and are an important part of the state's biodiversity and ecosystem services. As human population and development increase across the state, many lakes have been documented as nutrient impaired and are unable to maintain essential ecological functions. In this study, diatom diversity and functional groups in 72 Florida lakes were assessed for their relationships with associated water-quality data. Common alpha diversity indices, including Shannon-Weiner Index, the Gini-Simpson Index, and Hill’s numbers were utilized to measure trends in diatom communities, while the relationships between diatom lifeform characteristics and environmental data were explored using multivariate methods and …

March 2023: Region 10 Emerging Special Pathogen Treatment Center (Resptc) At Providence Sacred Heart Medical Center & Children’S Hospital, Providence - Special Pathogens Program

Special Pathogens Newsletters

REGION 10 SPECIAL PATHOGENS NEWSLETTER

Relationship Between Treatment Comorbidities And Hiv Viral Suppression Among People Who Live With Aids In Johannesburg., Nwogo Immaculata Ekeji, Tolulope A. Osoba, Hebatullah Tawfik, Mehdi Agha

Journal of Social, Behavioral, and Health Sciences

HIV has globally infected over 37.9 million people, of which 28.2 million (73%) are on antiretroviral treatment, and 66% of those on treatment are virally suppressed. In South Africa, however, low rate of viral suppression (47%) among people living with HIV is a major health problem that has continued to fuel HIV prevalence. A cross-sectional quantitative research design was used to investigate the relationship between treatment comorbidities and viral suppression among HIV-infected adults aged 18–49 who were diabetic, had cancer, or tuberculosis in Johannesburg. HIV Care Continuum formed the theoretical framework for this research. An existing HIV-infected patient de-identifiable dataset …

Tsks Localizes To Nuage In Spermatids And Regulates Cytoplasmic Elimination During Spermiation, Keisuke Shimada, Soojin Park, Seiya Oura, Taichi Noda, Akane Morohoshi, Martin M Matzuk, Masahito Ikawa

Faculty, Staff and Students Publications

Spermatozoa have a streamlined shape to swim through the oviduct to fertilize oocytes. To become svelte spermatozoa, spermatid cytoplasm must be eliminated in several steps including sperm release, which is part of spermiation. Although this process has been well observed, the molecular mechanisms that underlie it remain unclear. In male germ cells, there are membraneless organelles called nuage, which are observed by electron microscopy in various forms of dense material. Reticulated body (RB) and chromatoid body remnant (CR) are two types of nuage in spermatids, but the functions of both are unknown. Using CRISPR/Cas9 technology, we deleted the entire coding …

Human Schistosomiasis Vaccines As Next Generation Control Tools, Peter J Hotez, Maria Elena Bottazzi

Faculty, Staff and Students Publications

No abstract provided.

T-Cell Receptor Repertoire Sequencing In The Era Of Cancer Immunotherapy, Meredith L Frank, Kaylene Lu, Can Erdogan, Yi Han, Jian Hu, Tao Wang, John V Heymach, Jianjun Zhang, Alexandre Reuben

Faculty, Staff and Student Publications

T cells are integral components of the adaptive immune system, and their responses are mediated by unique T-cell receptors (TCR) that recognize specific antigens from a variety of biological contexts. As a result, analyzing the T-cell repertoire offers a better understanding of immune responses and of diseases like cancer. Next-generation sequencing technologies have greatly enabled the high-throughput analysis of the TCR repertoire. On the basis of our extensive experience in the field from the past decade, we provide an overview of TCR sequencing, from the initial library preparation steps to sequencing and analysis methods and finally to functional validation techniques. …

Public Health After Covid-19, Linda B. Cottler

Journal of Health Research

Background: This paper reflects on community health components during the COVID-19 epidemic in the light of major and always challenging health system issues from inequities, to financing, to delays in service delivery and the like.

Method: This is an invited commentary.

Results: The seven issues have been highlighted for the deconstruction of the public health infrastructure since COVID-19 (1) Growing social determinants of health disparities (2) Inadequate investment in public health infrastructure (3) Racial injustice and discrimination (4) Increased anxiety, depression and loneliness (5) Slow response to serious infectious diseases (6) Lack of diversity, equity, and sense of belonging (7) …

Graves' Disease And Major Histocompatibility Complex Class Ii: A Meta-Analysis Of Hla-Dq And Hla-Drb1, Dylan Thibaut, Connor Sweeney, Shannon South, Mohamed Hussein

Advances in Clinical Medical Research and Healthcare Delivery

Background: Human leukocyte antigen (HLA) class II has shown potential in determining prognosis, understanding medication reactions, and predicting onset of Graves’ disease. The aim of this study is to further investigate the association between Graves’ disease and HLA class II, specifically HLA-DQ and HLA-DR, via meta-analysis to find HLAs that can be further examined for prognostic reasons.

Methods: Statistical analysis was performed to determine if variants of HLA-DQA1, HLA-DQB1, or HLA-DRB1 were associated with significantly altered odds of Graves’ disease. A minimum of three studies pertaining to a particular HLA was required for inclusion. Studies were excluded if …

Money Matters: Time For Prevention And Early Intervention To Address Family Economic Circumstances, Nick Axford, Vashti Berry

Peninsula Medical School

Child poverty is associated with poorer physical and mental health, negative educational outcomes and adverse long-term social and psychological consequences, all of which impact on service demand and expenditure. Until now, however, prevention and early intervention practice has tended to focus on enhancing inter-parental relationships and parenting skills (e.g., via relationship skills education, home visiting, parenting programs, family therapy) or child language, social-emotional and life skills (e.g., early childhood education, school-based programs, youth mentoring). Programs often target low-income neighborhoods or families but rarely address poverty directly. While there is substantial evidence for the effectiveness of such interventions in improving child …

Shift To Online Learning: Response Of Pakistani Visual Art Teachers During Pandemic And Post-Covid Era, Tauseef Hussain Mr, Nimra Akram Miss, Rabiya Asim Ms, Amina Sarfraz Cheema Ms, Kiran Zohra Ms

Library Philosophy and Practice (e-journal)

This study aims to investigate the response of Visual art teachers to educational lockdown and shift to online learning at art institutions. The study employed qualitative phenomenological research design to investigate visual artists i.e., painters, sculptors, textile designers, graphic designers, and performing artists who were faculty members in five leading art institutions of Lahore. Data were collected by conducting a total of 15 interviews from each mentioned discipline. These interviews were conducted preferably in their studios, at their homes, or at times online through WhatsApp video calls. The data was analyzed thematically by using NVIVO 12 software.

Findings – It …

The P323l Substitution In The Sars-Cov-2 Polymerase (Nsp12) Confers A Selective Advantage During Infection, Hannah Goldswain, Xiaofeng Dong, Rebekah Penrice-Randal, Muhannad Alruwaili, Ghada T Shawli, Tessa Prince, Maia Kavanagh Williamson, Jayna Raghwani, Nadine Randle, Benjamin Jones, I'Ah Donovan-Banfield, Francisco J Salguero, Julia A Tree, Yper Hall, Catherine Hartley, Maximilian Erdmann, James Bazire, Tuksin Jearanaiwitayakul, Malcolm G Semple, Peter J M Openshaw, J Kenneth Baillie, Isaric4c Investigators, Stevan R Emmett, Paul Digard, David A Matthews, Lance Turtle, Alistair C Darby, Andrew D Davidson, Miles W Carroll, Julian A Hiscox

Faculty, Staff and Student Publications

BACKGROUND: The mutational landscape of SARS-CoV-2 varies at the dominant viral genome sequence and minor genomic variant population. During the COVID-19 pandemic, an early substitution in the genome was the D614G change in the spike protein, associated with an increase in transmissibility. Genomes with D614G are accompanied by a P323L substitution in the viral polymerase (NSP12). However, P323L is not thought to be under strong selective pressure.

RESULTS: Investigation of P323L/D614G substitutions in the population shows rapid emergence during the containment phase and early surge phase during the first wave. These substitutions emerge from minor genomic variants which become dominant …

Syndemics And Social Factors: Infectious Disease Patterns Within The Population Of People Experiencing Homelessness In The United States, Kathleen Berzonsky

Honors Program: Senior Projects (Public)

Individuals experiencing homelessness are at increased risk of suffering from infectious diseases. This is due to a number of social factors and healthcare disparities, as well as the idea of syndemics, by which diseases cluster together to worsen disease burden. Current intervention strategies approach treatment from a post-infection perspective, but reducing transmission rates of infectious diseases within the population of people experiencing homelessness will require a shift in the healthcare framework. The issue of people experiencing homelessness must be viewed through a biosocial lens, focusing on preventative care and treatment. I provide an overview of the social factors governing infectious …

The Spanish Flu Of 1918: A Historical Reflection And Lessons, Jonathan Kopel

Journal of Community Hospital Internal Medicine Perspectives

A catastrophic Spanish flu epidemic spread throughout the world throughout 1918 and part of 1919. In the spring of 1918, an army training center at the Fort Riley Kansas found the first cases of Spanish influenza in the United States. The first incidence of the virus in Kansas was quite moderate, with low-fever, nausea, headache, and fatigue. The Spanish grip took an ominous turn in the fall of 1918. The moderate United States (U.S.) strain traveled on troops and several ferrying boats to Europe. The injured and the ill were sent back home, spreading freely throughout the U.S. the dangerous …

A Case Of Candida Empyema In A Patient With Esophagopleural Fistula, Ramez Alyacoub

Journal of Community Hospital Internal Medicine Perspectives

Fungal empyema is a rare entity, particularly in immunocompetent patients. It has been noted to occur in a patient with esophageal perforation. Esophageal perforation has a wide range of clinical presentations and associated complications depending on the size and site of perforation. Although the classic presentation of esophageal perforation, also known as Boerhaave syndrome, is often dramatic with hemodynamic instability and mediastinitis. Smaller perforations and esophageopleural fistula can lead to more indolent presentation in the form of complications such as necrotizing pneumonia and pleural effusions. Here we present a 42-year-old patient with alcohol withdrawal and aspiration pneumonia, later found to …

Case Report Of Acute Q Fever With Hepatitis Progressing To Chronic Q Fever With Endocarditis, Abbas B. Jama, Jessica L. Sheehy, Hassan Mohamed, Noura Attallah, Esraa Hassan, Anwar Khedr, Hisham Mushtaq, Omar Y. Mousa, James J. Milavetz, Ali Sadik, Mohamad El Labban, Nitesh Jain, Salim Surani, Eric O. Gomez Urena, Syed Anjum Khan

Journal of Community Hospital Internal Medicine Perspectives

A 35-year-old male greenhouse worker presented with myalgia, fatigue, and fever. Initially, he was thought to have an unspecified viral infection and was treated with conservative therapy. However, the patient’s symptoms persisted, and he reported additional symptoms of mild abdominal pain and headaches. Laboratory evaluation was significant for elevated liver enzymes. Due to concern for acute hepatitis and persistent fever the patient was hospitalized. During his hospital course, no infectious etiology was found to explain his symptoms. After discharge from the hospital, additional testing showed positive serology for Q fever IgG phase II antibody (1:8192) and phase II antibody IgM …

Recent Developments In Detection And Therapeutic Approaches For Antibiotic-Resistant Bacterial Infections, Kavya Moorthy, Kai-Chih Chang, Hsueh-Hui Yang, Wen-Min Su, Cheng-Kang Chiang, Zhiqin Yuan

Journal of Food and Drug Analysis

Owing to the widespread emergence and proliferation of antibiotic-resistant bacteria, the therapeutic benefits of antibiotics have been reduced. In addition, the ongoing evolution of multidrug-resistant pathogens poses a challenge for the scientific community to develop sensitive analytical methods and innovative antimicrobial agents for the detection and treatment of drug-resistant bacterial infections. In this review, we have described the antibiotic resistance mechanisms that occur in bacteria and summarized the recent developments in detection strategies for monitoring drug resistance using different diagnostic methods in three aspects, including electrostatic attraction, chemical reaction, and probe-free analysis. Additionally, to understand the effective inhibition of drug-resistant …

Checks & Balances In The Bayou State: Louisiana’S Constitutional Balance Of Power Between The Executive And Legislative Branches During States Of Emergency, Luke A. Dupré

Louisiana Law Review

No abstract provided.

A Multiscale Mechanistic Model Of Human Dendritic Cells For In-Silico Investigation Of Immune Responses And Novel Therapeutics Discovery, Sara Sadat Aghamiri, Bhanwar Lal Puniya, Rada Amin, Tomáš Helikar

Department of Biochemistry: Faculty Publications

Dendritic cells (DCs) are professional antigen-presenting cells (APCs) with the unique ability to mediate inflammatory responses of the immune system. Given the critical role of DCs in shaping immunity, they present an attractive avenue as a therapeutic target to program the immune system and reverse immune disease disorders. To ensure appropriate immune response, DCs utilize intricate and complex molecular and cellular interactions that converge into a seamless phenotype. Computational models open novel frontiers in research by integrating large-scale interaction to interrogate the influence of complex biological behavior across scales. The ability to model large biological networks will likely pave the …

Affected Family Members' Communicative Management Of Opioid Misuse Stigma: Applying And Rethinking The Stigma Management Communication Typology, Sydney O'Shay, Emily Pasman, Danielle L. Hicks, Suzanne Brown, Elizabeth Aguis, Stella M. Resko

Communication Studies and Philosophy Faculty Publications

Opioid misuse is a prevalent health problem in the United States with consequences extending past the person who misuses opioids to affected family members (AFM) through courtesy stigma. The goals of this study were to understand the stigma management communication (SMC) strategies employed by AFMs when they experience courtesy stigma and changes in strategies used over time. The findings from interviews with 34 AFMs suggest the SMC strategies they employ range from those that indicate acceptance of stigma and avoidance of stigma situations to strategies where AFMs actively challenge opioid misuse stigma. However, strategy use depended on the social context …

Affected Family Members’ Communicative Management Of Opioid Misuse Stigma: Applying And Rethinking The Stigma Management Communication Typology, Sydney O'Shay, Emily Pasman, Danielle L. Hicks, Suzanne Brown, Elizabeth Aguis, Stella M. Resko

Publications

Opioid misuse is a prevalent health problem in the United States with consequences extending past the person who misuses opioids to affected family members (AFM) through courtesy stigma. The goals of this study were to understand the stigma management communication (SMC) strategies employed by AFMs when they experience courtesy stigma and changes in strategies used over time. The findings from interviews with 34 AFMs suggest the SMC strategies they employ range from those that indicate acceptance of stigma and avoidance of stigma situations to strategies where AFMs actively challenge opioid misuse stigma. However, strategy use depended on the social context …

Don’T Make Them Martyrs: Empowering Children In The Foster Care & Juvenile Justice Systems Through Covid-19 Vaccine Consenting Rights, Victoria Kalumbi

Minnesota Journal of Law & Inequality

No abstract provided.

Credit Suisse Announces Technical Delay Of Publication Of 2022 Annual Report, Credit Suisse

Documents

No abstract provided.

Of Mētis And Cuttlefish: Employing Collective Mētis As A Theoretical Framework For Marginalized Communities, Justiss Wilder Burry

USF Tampa Graduate Theses and Dissertations

This LGBTQ+ rhetoric of health and medicine (RHM) community-based research explores the ways community members and queer community participants communicate about potential intimate partner selection in Central Florida. The dissertation introduces the theoretical framework of “collective mētis” as a way to analyze and discuss community communication against Florida’s required HIV disclosure laws that attempt to medicalize this and control this community’s intimate partner selection. Research questions include: 1) How do people in counterpublic enclaves (Chávez, 2011) communicate their potential intimate partner desires to others in the community including the types of disclosures they express and negotiate, and how? 2) How …

Biased But Reasonable: Bias Under The Cover Of Standard Of Care, Maytal Gilboa

Georgia Law Review

Inequities in the distribution of healthcare are widely acknowledged to plague the United States healthcare system. Controversies as to whether anti-discrimination law allows individuals to bring lawsuits with respect to implicit rather than intentional bias render negligence law an important avenue for redressing harms caused by implicit bias in medical care. Yet, as this Article argues, the focus of negligence law on medical standards of care to define the boundaries of healthcare providers’ legal duty of care prevents the law from adequately deterring implicit bias and leaves patients harmed by biased treatment decisions without redress for their losses, so long …

Whole Transcriptome-Based Skin Virome Profiling In Typical Epidermodysplasia Verruciformis Reveals Α-, Β-, And Γ-Hpv Infections, Amir Hossein Saeidian, Leila Youssefian, Mahtab Naji, Hamidreza Mahmoudi, Samantha M Barnada, Charles Huang, Karim Naghipoor, Amir Hozhabrpour, Jason S Park, Flavia Manzo Margiotta, Fatemeh Vahidnezhad, Zahra Saffarian, Kambiz Kamyab-Hesari, Mohammad Tolouei, Niloofar Faraji, Seyyede Zeinab Azimi, Ghazal Namdari, Parvin Mansouri, Jean-Laurent Casanova, Vivien Béziat, Emmanuelle Jouanguy, Jouni Uitto, Hassan Vahidnezhad

Department of Dermatology and Cutaneous Biology Faculty Papers

HPVs are DNA viruses include approximately 450 types that are classified into 5 genera (α-, β-, γ-, μ-, and ν-HPV). The γ- and β-HPVs are present in low copy numbers in healthy individuals; however, in patients with an inborn error of immunity, certain species of β-HPVs can cause epidermodysplasia verruciformis (EV), manifesting as recalcitrant cutaneous warts and skin cancer. EV presents as either typical or atypical. Manifestations of typical EV are limited to the skin and are caused by abnormal keratinocyte-intrinsic immunity to β-HPVs due to pathogenic sequence variants in TMC6, TMC8, or CIB1. We applied a transcriptome-based computational pipeline, …