Digital Commons Network^™

Trpm5'S Role In Vascular Development, Megan Goeckel

Arts & Sciences Graduate Student Theses and Dissertations

Blood vessels are composed of two main cell types, endothelial cells (ECs) and mural cells (MCs). Endothelial cells comprise of the inner-most layer of the vessel and sense changes in blood flow to recruit mural cells. The mechanism for how endothelial cells sense these hemodynamic changes is not well understood and deciphering this mechanism is the focus of this thesis. We hypothesize that transient receptor potential melastatin 5 (TRPM5), a transmembrane channel protein, is involved in this process. Here, using cultured human endothelial cells and the zebrafish model, we show that inhibition of TRPM5 decreases vessel formation and increases EC-MC …

Effect Of Obesity And Insulin Resistance On Glucose And Fatty Acid Metabolism, Chao Cao

WUSM Theses and Dissertations – All Programs

In people with obesity, insulin resistance of glucose and fatty acid metabolism is common and it is thought that insulin resistance of fatty acid metabolism is involved in causing insulin resistance of glucose metabolism and subsequently hyperglycemia through a mechanism referred to as “lipotoxicity”. However, in many individuals with obesity and insulin resistance fasting plasma glucose and free fatty acid (FFA) concentrations are not elevated. It is widely believed that pancreatic beta-cells detect the need to increase insulin secretion in order to compensate for the impaired insulin action caused by insulin resistance in these people, whereas beta-cell dysfunction results in …

The Echo: May 13, 2024, Taylor University

2023-2024 (Volume 111)

No abstract provided.

Equivalent Running Leg Lengths Require Prosthetic Legs To Be Longer Than Biological Legs During Standing, Janet H. Zhang-Lea, Joshua R. Tacca, Owen N. Beck, Paolo Taboga, Alena M. Grabowski

Human Physiology Faculty Scholarship

We aimed to determine a method for prescribing a standing prosthetic leg length (ProsL) that results in an equivalent running biological leg length (BioL) for athletes with unilateral (UTTA) and bilateral transtibial amputations (BTTA). We measured standing leg length of ten non-amputee (NA) athletes, ten athletes with UTTA, and five athletes with BTTA. All athletes performed treadmill running trials from 3 m/s to their maximum speed. We calculated standing and running BioL and ProsL lengths and assessed the running-to-standing leg length ratio (Lratio) at three instances during ground contact: touchdown, mid-stance, and take-off. Athletes with UTTA had 2.4 cm longer …

Contributions To Auditory System Conduction Velocity: Insights With Multi-Modal Neuroimaging And Machine Learning In Children With Asd And Xyy Syndrome, Jeffrey I. Berman, Luke Bloy, Lisa Blaskey, Carissa R. Jackel, Judith S. Miller, Judith Ross, J. Christopher Edgar, Thimothy P.L. Roberts

Department of Pediatrics Faculty Papers

Introduction: The M50 electrophysiological auditory evoked response time can be measured at the superior temporal gyrus with magnetoencephalography (MEG) and its latency is related to the conduction velocity of auditory input passing from ear to auditory cortex. In children with autism spectrum disorder (ASD) and certain genetic disorders such as XYY syndrome, the auditory M50 latency has been observed to be elongated (slowed).

Methods: The goal of this study is to use neuroimaging (diffusion MR and GABA MRS) measures to predict auditory conduction velocity in typically developing (TD) children and children with autism ASD and XYY syndrome.

Results: Non-linear TD …

The Effect Of Training Status On Exercise Performance And Oxygen Uptake Responses To Beetroot Juice Supplementation In Cyclists, Haley Mcvannel

Masters Theses, 2020-current

Purpose: To determine if cardiovascular fitness, assessed by peak oxygen consumption (VO_2peak),influences the physiological and performance effects of beetroot juice (BRJ) supplementation and to explore if recent training habits independently influence this response. Methods: Thirteen male and female cyclists completed two graded exercise tests to assess cardiovascular fitness and four, seven-day experimental phases. Five subjects were classified as high fitness (VO_2peak: 65.9 ± 4.5 mL∙kg^-1∙min^-1) and eight as lower fitness (VO_2peak: 44.2 ± 7.0 mL∙kg^-1∙min^-1). Subjects consumed 140 ml of concentrated BRJ or placebo the last …

Approximate And Sample Entropy Of Center Of Pressure In Unperturbed Tandem Standing: Contribution Of Embedding Dimension And Tolerance, Jayla Mashae Wesley

Masters Theses

Approximate entropy (ApEn) and sample entropy (SampEn) are statistical methods designed to quantify the regularity or predictability of a time series. Although ApEn has been a prominent choice for use, it is currently unclear as to which method and parameter selection combination is optimal for its application in biomechanics. The goal of this thesis was to examine the difference between ApEn and SampEn related to center of pressure (COP) data during standing balance tasks, while also refining tolerance r, to determine entropy optimization. Six participants completed five 30-second, feet together and tandem standing, trials under eyes-open and eyes-closed conditions. Ground …

Evaluating The Impact Of Routine Enteral Iron Fortification On Gastrointestinal Dysbiosis In Premature Infants, Matthew Van Ormer

Theses & Dissertations

The gastrointestinal microbiome in preterm infants bears significant influence on optimal outcomes – dysbiosis is shown to substantially increase the risk of the life-threatening necrotizing enterocolitis, whereas optimal microbiome composition improves nutrient digestion and immune function. Iron is a vital nutrient especially during the perinatal window of rapid hemoglobin production, tissue growth, and foundational neurological development. However, excess colonic iron exhibits potent oxidation capacity and alters the competitive environment of microbes – potentially facilitating the proliferation of pathological bacterial strains in the gut. Of note, breastfed preterm infants routinely receive iron fortification starting at 14 days of life and are …

Species Differences In Platelet Protease-Activated Receptors, Stephanie A Renna, Steven E. Mckenzie, James V. Michael

Cardeza Foundation for Hematologic Research

Protease-activated receptors (PARs) are a class of integral membrane proteins that are cleaved by a variety of proteases, most notably thrombin, to reveal a tethered ligand and promote activation. PARs are critical mediators of platelet function in hemostasis and thrombosis, and therefore are attractive targets for anti-platelet therapies. Animal models studying platelet PAR physiology have relied heavily on genetically modified mouse strains, which have provided ample insight but have some inherent limitations. The current review aims to summarize the notable PAR expression and functional differences between the mouse and human, in addition to highlighting some recently developed tools to further …

Ungrading Writing: Changes In Motivation, Volition, And Perceived Learning, Jennifer B. Wilson

Electronic Theses and Dissertations Archive

Motivation and volition are critical precursors to learning, as students learn best when the course material is relevant and personally meaningful. The ARCS-V (i.e., attention, relevance, confidence, satisfaction, and volition) model addresses motivation from an instructional design perspective. Underlying the model is a learner-centered locus of control. Research suggests that grades can be a barrier to learning because they are teacher-centered, allowing instructors to control the reward and punishment cycle. When grades are given, earning high marks often replaces learning as the educational goal. In response to this concern, many undergraduate composition instructors have implemented ungrading, an assessment method in …

Effect Of High Intensity Interval Training Under Mask On Force Vital Capacity In Football Players, Neranoch Bumrung, Apiwan Manimmanakorn, Natthapon Traiperm

International Journal of Exercise Science

International Journal of Exercise Science 16(6): 576-586, 2023. The purpose of the study was to investigate the effect of high interval training (HIIT) under mask on forced vital capacity in football players. Fourteen male football players (age 20 ± 2 years) were randomized into the without mask group (CON) or with the 2 difference mask groups [Surgical mask (SM), FFP2 mask (FM)]. HIIT program were implemented six movements in each session three times per week. Body composition, forced vital capacity and percutaneous oxygen saturation test were taken before and after 4 weeks intervention. The results show that forced vital capacity …

Key Takeaways For Knowledge Expansion Of Early-Career Scientists Conducting Transdisciplinary Research In Energetics And Cancer (Trec): A Report From The Trec Training Workshop 2022, Che-Pei Kung, Meghan B. Skiba, Erika J. Crosby, Jessica Gorzelitz, Mary A. Kennedy, Bethany A. Kerr, Yun Rose Li, Sarah Nash, Melanie Potiaumpai, Amber S. Kleckner, Dara L. James, Michael F. Coleman, Ciaran M. Fairman, Gloria C. Galván, David O. Garcia, Max J. Gordon, Mathilde His, Lyndsey M. Hornbuckle, So-Youn Kim, Tae-Hyung Kim, Amanika Kumar, Mélanie Mahé, Karen K. Mcdonnell, Jade Moore, Sangphil Oh, Xinghui Sun, Melinda L. Irwin

Pathology Research and Scholarship

The overall goal of the annual Transdisciplinary Research in Energetics and Cancer (TREC) Training Workshop is to provide transdisciplinary training for scientists in energetics and cancer and clinical care. The 2022 Workshop included 27 early-to-mid career investigators (trainees) pursuing diverse TREC research areas in basic, clinical, and population sciences. The 2022 trainees participated in a gallery walk, an interactive qualitative program evaluation method, to summarize key takeaways related to program objectives. Writing groups were formed and collaborated on this summary of the 5 key takeaways from the TREC Workshop. The 2022 TREC Workshop provided a targeted and unique networking opportunity …

Does Vocalization Increase The Positive Valence Of Emotion?, Joshua Hyde

Electronic Theses and Dissertations Archive

This study aims to determine if the act of voicing can have an effect on mood in a within-participant reversal paradigm, where each participant served as their own control. Following a baseline condition, participants underwent three experimental conditions: breathing, articulating, and voicing. Following baseline and experimental conditions, participants underwent an emotion-induction paradigm by looking at pictures. They rated their current mood and arousal levels following each picture. Additionally, psychophysiological measures of facial electromyography, electrocardiography, and electrodermal responses were gathered throughout the experiment. Results were mixed, revealing that there was a mood change during aversive picture viewing toward positive moods in …

Heart Rhythm And Related Cardiac Conditions, Lucas Joseph Asadorian

Honors Capstone Projects and Theses

No abstract provided.

The Role Of Cytochrome P450 2e1 In Hepatic Ischemia Reperfusion Injury And Reactive Oxygen Species Formation, Nouf Alwadei

Pharmaceutical Sciences (PhD) Dissertations

The mechanisms of hepatic ischemia/reperfusion (I/R) injury, which occurs during liver transplantation or surgery, are poorly understood. The central pathophysiological phenomenon of this injury is the formation of reactive oxygen species (ROS). Recent studies suggest that P450 enzymes may contribute to ROS generation, which may occur through their uncoupling and/or release of heme and iron after their degradation. Among P450 enzymes, CYP2E1 is unique because it is considered a leaky enzyme, producing significant ROS through uncoupling in the absence of substrates, accelerating its own degradation and reducing its half-life. In this dissertation, we generated and characterized a HepG2 cell line …

Pharmacy Matters - May 2023, University Of Mississippi. School Of Pharmacy

Pharmacy Matters Newsletters

Featured stories: "Natural Products Center Hosts 21st International Botanicals Conference," "Distinguished Teaching Scholars Announced," "Alumni Honored at Annual Banquet," "Hayes, Khan Represent Ph.D. Graduates as Class Marshals," "Journey to Commencement: Passion for Politics, Policy and Professional Advocacy," "Pharmacy School Pioneers Receive Recognition," "Minority Association Sponsors Gordon Mental Health Research Talk"

Contributors, Honorable Mentions, Sponsors

The Oval

No abstract provided.

Rats Lacking Ucp1 Present A Novel Translational Tool For The Investigation Of Thermogenic Adaptation During Cold Challenge, Jaycob D. Warfel, Carrie M. Elks, David S. Bayless, Bolormaa Vandanmagsar, Allison C. Stone, Samuel E. Velasquez, Paola Olivares-Nazar, Robert C. Noland, Sujoy Ghosh, Jingying Zhang, Randall L. Mynatt

Division of Basic Science Faculty Publications

Aim: Valuable studies have tested the role of UCP1 on body temperature maintenance in mice, and we sought to knockout Ucp1 in rats (Ucp1−/−) to provide insight into thermogenic mechanisms in larger mammals. Methods: We used CRISPR/Cas9 technology to create Ucp1−/− rats. Body weight and adiposity were measured, and rats were subjected to indirect calorimetry. Rats were maintained at room temperature or exposed to 4°C for either 24 h or 14 days. Analyses of brown and white adipose tissue and skeletal muscle were conducted via histology, western blot comparison of oxidative phosphorylation proteins, and qPCR to compare mitochondrial DNA levels …

Investigating Microbial Metabolism Of Garcinia Mangostana By The Human Gut Bacterium Clostridium Sporogenes, Anna Liu

Honors Scholar Theses

The human gut microbiome is a burgeoning field of research due to recent recognition of its profound impact on the human hosts’ homeostasis, physiology and metabolism, and disease. Botanical natural products have been utilized for disease prevention and medical treatment since ancient times. Increased consumption of botanical natural products for their health benefits have led researchers to investigate interactions between the gut microbiome and botanical natural products. Garcinia mangostana, also known as mangosteen, is a fruit from Southeast Asia that is known for its health benefits such as anti-inflammatory, antihypertensive, and anticancer properties. Its benefits have been linked to bioactive …

The Impact Of Low Appendicular Skeletal Muscle Mass In The Development Of Insulin Resistance And Metabolic Syndrome: A Systematic Review Of The Literature, Ella Boston

The Eleanor Mann School of Nursing Undergraduate Honors Theses

Chronic conditions are the leading cause of death and disability. More specifically, cardio-metabolic disease is the number one cause of death worldwide. The tragedy and burden of disease is unrefuted; even more so, evidence indicates that realistic modifications of lifestyle can prevent most chronic disease. Metabolic syndrome and insulin resistance are leading risk factors in the development of cardio-metabolic disease. Presently, chronic disease is treated through symptomatic evaluation of risk. Reframing the lens of care through root cause evaluation is fundamental to optimizing patient outcomes. The purpose of this systematic review is to synthesize literature evaluating the impact of low …

Physiological Effects Of A Traditional Active Warm-Up Versus A Passive Warm-Up During Submaximal Endurance Exercise, Vernice Ollano

UNLV Theses, Dissertations, Professional Papers, and Capstones

The purpose of this study is to investigate physiological responses during a traditional active warm-up and a passive warm-up consisting of wearing heated trousers during a submaximal endurance cycling workout. Participants included 10 cyclists/triathletes (3 females, 7 males; age: 48 ± 13 years old; mass: 157.9 ± 33.8 kg; height: 169.3 ± 8.65 cm; percent body fat: 25.3 ± 8.53 %) of at least 18 years of age who could continuously cycle for at least 120 minutes and had knowledge of their individual FTP. Experimental 1-day testing included a randomized crossover design where participants proceeded with two different protocols: an …

Fatty Females And Muscular Males: Investigating Human Sexual Dimorphism Across The Upper And Lower Skeleton, Brooklin Edwards

Anthropology Undergraduate Honors Theses

The sexual dimorphism profile of human body composition produces a unique pattern. Compared to other primates, humans have a mild size dimorphism; however, human males have a particularly muscular upper body and human females have a permanent fatty composition. These findings have resulted in varying interpretations related to sexual selection, mating systems, and male competition. Using humerus and femur measurements collected from 9 primate species, we investigated how the pronounced sexually dimorphic tissue composition of humans influences skeletal elements compared to the other primates. We hypothesized that over the course of human evolution, human females developed a body composition that …

Characterization Of Leptin Receptor Expressing Cells In The Cortex And Hippocampus Of The Mouse Brain, Cade Canepa

Honors Capstones

No abstract provided.

A Pathophysiological, Clinical, And Epidemiological View Of Malaria, Alyssa Watt

Senior Honors Theses

Malaria is a parasitic disease that is common among all ages in tropical and subtropical countries. Annually, there are an estimated 3.3 billion people in 97 countries at risk of contracting malaria. Malaria has been a major global health problem throughout history and is a leading cause of death and disease for many within tropical regions. In the past decade, efforts, such advances in medicine and insecticide techniques, have reduced the prevalence of malaria by 50% which suggests that the elimination of this disease is possible. With the prevalence of malaria and recent reduction, it is vital to aid in …

A Retrospective Analysis Of Rabies Lyssavirus Animal Testing In Nebraska, 2012 Through 2021, Elijah Theye

Capstone Experience: Master of Public Health

Background: Rabies is a zoonotic disease caused by a neurotropic virus that has been a public health issue for over 4,000 years, killing roughly 59,000 humans globally each year. This study examined the seasonality of bat-human exposures, the association between animal-human exposure types and animal rabies positivity, and using skunk monthly positivity rates to predict all non-skunk non-bat animal positivity rates with a lag time of six months, in Nebraska.

Methods: Animal rabies testing data from 2012-21 were extracted from Nebraska’s Electronic Disease Surveillance System. Animal tests that had a valid animal rabies test were included in the analyses. To …

Genetic Associations Between Modifiable Risk Factors And Alzheimer Disease, Jiao Luo, Jesper Qvist Thomassen, Céline Bellenguez, Benjamin Grenier-Boley, Itziar De Rojas, Atahualpa Castillo, Kayenat Parveen, Fahri Küçükali, Aude Nicolas, Oliver Peters, Anja Schneider, Martin Dichgans, Dan Rujescu, Norbert Scherbaum, Deckert Jürgen, Steffi Riedel-Heller, Lucrezia Hausner, Laura Molina Porcel, Emrah Düzel, Timo Grimmer, Jens Wiltfang, Stefanie Heilmann-Heimbach, Susanne Moebus, Thomas Tegos, Nikolaos Scarmeas, Jordi Clarimon, Fermin Moreno, Jordi Pérez-Tur, María J Bullido, Pau Pastor, Raquel Sánchez-Valle, Victoria Álvarez, Mercè Boada, Pablo García-González, Raquel Puerta, Pablo Mir, Luis M Real, Gerard Piñol-Ripoll, Jose María García-Alberca, Jose Luís Royo, Eloy Rodriguez-Rodriguez, Hilkka Soininen, Teemu Kuulasmaa, Alexandre De Mendonça, Shima Mehrabian, Jakub Hort, Martin Vyhnalek, Sven Van Der Lee, Caroline Graff, Goran Papenberg, Vilmantas Giedraitis, Anne Boland, Delphine Bacq-Daian, Jean-François Deleuze, Gael Nicolas, Carole Dufouil, Florence Pasquier, Olivier Hanon, Stéphanie Debette, Edna Grünblatt, Julius Popp, Luisa Benussi, Daniela Galimberti, Beatrice Arosio, Patrizia Mecocci, Vincenzo Solfrizzi, Lucilla Parnetti, Alessio Squassina, Lucio Tremolizzo, Barbara Borroni, Benedetta Nacmias, Sandro Sorbi, Paolo Caffarra, Davide Seripa, Innocenzo Rainero, Antonio Daniele, Carlo Masullo, Gianfranco Spalletta, Julie Williams, Philippe Amouyel, Frank Jessen, Patrick Kehoe, Magda Tsolaki, Giacomina Rossi, Pascual Sánchez-Juan, Kristel Sleegers, Martin Ingelsson, Ole A Andreassen, Mikko Hiltunen, Cornelia Van Duijn, Rebecca Sims, Wiesje Van Der Flier, Agustín Ruiz, Alfredo Ramirez, Jean-Charles Lambert, Ruth Frikke-Schmidt

Faculty, Staff and Students Publications

IMPORTANCE: An estimated 40% of dementia is potentially preventable by modifying 12 risk factors throughout the life course. However, robust evidence for most of these risk factors is lacking. Effective interventions should target risk factors in the causal pathway to dementia.

OBJECTIVE: To comprehensively disentangle potentially causal aspects of modifiable risk factors for Alzheimer disease (AD) to inspire new drug targeting and improved prevention.

DESIGN, SETTING, AND PARTICIPANTS: This genetic association study was conducted using 2-sample univariable and multivariable mendelian randomization. Independent genetic variants associated with modifiable risk factors were selected as instrumental variables from genomic consortia. Outcome data for …

An Analysis Of Animal Testing In Medical Research And Ethical Considerations, Diana Santini

Academic Festival

Introduction:

The use of animal testing in medical research has been the gold standard for gathering information and determining safety and efficacy for pharmaceutical candidates. While animal testing has been considered an essential component in medical research, evidence has shown that it has become an ethical issue in not only animals, but humans as well, delaying the development of medical treatments and providing inaccurate predictions of safety use in human administration.

The Minutes Of The Marshall University Graduate Council Meeting, January 27, 2023, Marshall University Graduate Council

Graduate Council Minutes

No abstract provided.

Effects Of Cannabichromene On Pain And Neuroinflammation In Hiv-1 Tat Transgenic Mice, Sydney Wheeler

Honors Theses

HIV-1 TransActivator of Transcription (Tat) is a vital regulatory protein involved in HIV replication and implicated in development of HIV-associated pain and neurocognitive deficits. Tat is thought to contribute to these pathologies through proinflammatory pathways. Developing mechanisms to curb Tat-mediated inflammation is a key step in improving the quality of life of those living with HIV. Both recreational and medicinal cannabis use is prevalent among many people living with HIV; given this prevalence and the perceived anti-inflammatory benefits of cannabis use, it is important to investigate how chemical constituents of cannabis may impact HIV. In the present study, the effects …

Greenland Shark Lifespan And Implications For Human Longevity, Holly C. Gates

Honors Capstone Projects and Theses

No abstract provided.