Open Access. Powered by Scholars. Published by Universities.®

Digital Commons Network™

Open Access. Powered by Scholars. Published by Universities.®

2023

Discipline
Institution
Keyword
Publication
Publication Type
File Type

Articles 2221 - 2250 of 24881

Full-Text Articles in Entire DC Network

The Guardian The Month Of December 2023, Wright State Student Body Dec 2023

The Guardian The Month Of December 2023, Wright State Student Body

The Guardian Student Newspaper

News articles from The Guardian for the Month of December 2023. The Guardian is the official student-run newspaper for Wright State University. It has been published regularly since March of 1965.


Teaching Animal And Plant Survival With Instructional And Assessment Strategies, Kapri Case Dec 2023

Teaching Animal And Plant Survival With Instructional And Assessment Strategies, Kapri Case

Theses/Capstones/Creative Projects

This capstone study explored the impact of instructional and assessment strategies on students’ achievement in science. The study spanned 6 days, comprising 5 lessons. The students took a 6-item pre-assessment to assess prior knowledge, a 4-item post-assessment at the unit’s conclusion, and a summative assessment to demonstrate their new knowledge.

Instructional strategies were adapted based on pre-assessment results. Analysis of student performance data revealed a marked increase in learning outcomes through the integration of differentiated instruction and assessment strategies. Following the review of the student work, it became evident that the integration of summarizing/note-taking and cooperative learning significantly enhanced post-instruction …


Monitoring The Effectiveness Of Wildlife Exits And Bobcat Use Along A South Texas Highway, Jamie E. Langbein Dec 2023

Monitoring The Effectiveness Of Wildlife Exits And Bobcat Use Along A South Texas Highway, Jamie E. Langbein

Theses and Dissertations

Roadside exclusion fencing is commonly used to mitigate wildlife road mortalities and property damages caused by wildlife-vehicle collisions. A potential consequence is that animals may become trapped on the road by fencing. On State Highway 100 in South Texas, the Texas Department of Transportation installed ten wildlife exits (WE) allowing animals to exit the roadway through a modified opening in the fence. However, some individuals used the WE to access the roadway. In this study, a new design was implemented, with narrower openings, a ramp and a clear plastic one-way door. While the updated design prevented road access for wildlife, …


Status Of The Western Australian Pastoral Rangelands 2023: Total Vegetative Cover And Cover Risk, Department Of Primary Industries And Regional Development Dec 2023

Status Of The Western Australian Pastoral Rangelands 2023: Total Vegetative Cover And Cover Risk, Department Of Primary Industries And Regional Development

Natural resources published reports

The Department of Primary Industries and Regional Development (DPIRD) monitors and reports on the vegetation condition of pastoral rangelands in Western Australia. This 2023 short report uses remotely sensed total cover data available to the end of October 2023, rainfall data to the end of November 2023 and Stock Return data reported in 2022 (the most recent available) to determine cover risk. Cover risk at the land conservation district (LCD) level is an indicator of the likelihood of total cover declining to, or remaining at, low or very low levels. Total cover and cover risk may not be indicative of …


Fabrication Of Pvc Enrofloxacin-Selective Electrodes For Estimating Enrofloxacin In Pure Form And As Preparation Formula, Omar Salih Hassan, Sahar S M Alabdullah, Amina M. Abass, Ahmed Ahmed Dec 2023

Fabrication Of Pvc Enrofloxacin-Selective Electrodes For Estimating Enrofloxacin In Pure Form And As Preparation Formula, Omar Salih Hassan, Sahar S M Alabdullah, Amina M. Abass, Ahmed Ahmed

Baghdad Science Journal

This study explored the development and qualities of the response of electrochemical properties of enrofloxacin-selective electrodes using precipitation based on producing phosphotungstic, after utilizing a matrix of polyvinyl chloride (PVC) and dibutyl phthalate or dibutyl phosphate as a plasticizer. The resulting membrane sensors were an enrofloxacin-phosphotungstic electrode (sensors 1) and an ENR-DOP-PTA electrode (sensors 2). Linear responses of (ENR-DBPH-PTA) and (ENR-DOP-PTA) within the concentration ranges of 2.1×10-6-10-1 and 3.0×10-6-10-2 mol. L-1, respectively, for both sensors were observed. Slopes of 51.61±0.24 and 39.40± 0.16 mV/decade and pH ranges equal to 2.5-8.5 and 2.0-9.0 were observed for sensors 1 and 2, respectively. …


Assessing The Feasibility Of Implementing A Dairy Industry In Mauritius, Sophia Ultes Dec 2023

Assessing The Feasibility Of Implementing A Dairy Industry In Mauritius, Sophia Ultes

Economics Undergraduate Honors Theses

Mauritius faces food security and economic challenges due to its lack of a domestic dairy industry. To address this, the University of Arkansas partnered with the Ministry of Agro Industry and Food Security to explore the possibility of establishing a dairy sector on the island.

Through surveys and interviews with locals, including restaurant owners and grocery store customers, we investigated the demand for fresh milk. We also studied factors like consumer preferences and production costs. Our research, conducted with IRB approval, aimed to understand if people are willing to buy locally produced milk.

During the summer, our team visited Mauritius …


Post-Pandemic Student Attendance, Becki Delloyd Dec 2023

Post-Pandemic Student Attendance, Becki Delloyd

Dissertations

Student attendance has been a national concern since the start of compulsory education in America; however, since the COVID-19 pandemic, attendance plummeted to never- before-seen attendance rates. Nationally, by the end of the 2021-2022 school year, nearly one in three students were absent 10% or more of the school year (Attendance Works, 2023). My 39-question survey conducted in two vastly different states between April and May of 2023 corroborated the national data and resulted in nearly one in three students being absent 10 or more school days in a year. Through my study, I explored parents’ perceptions of post-pandemic K–12 …


Madonnas Of The Prairie, James C. Schaap Dec 2023

Madonnas Of The Prairie, James C. Schaap

Pro Rege

No abstract provided.


Disrupting Childhood Trauma With Holistic Health Practices In Low Ses Elementary Schools, Unna G. Burns, Ketzia Kogan, Sarah M. Wingerden, Hannah Meck Dec 2023

Disrupting Childhood Trauma With Holistic Health Practices In Low Ses Elementary Schools, Unna G. Burns, Ketzia Kogan, Sarah M. Wingerden, Hannah Meck

Psychology and Child Development

It is evident that adverse childhood experiences (ACE) are a large challenge to tackle in the U.S., with 61% of adults reporting that they’ve experienced one ACE and 16% reporting four or more ACEs by age 18 (Centers for Disease Control and Prevention, 2019). ACE score items include, but are not limited to experiencing verbal, physical, sexual abuse, physical and/or emotional neglect, having a mentally ill family member, witnessing violence in the home towards a parent, and/or having a family member in prison. Such experiences serve as a public health threat that, without early intervention, bring about long-term effects biologically, …


Docai, Riley Badnin, Justin Brunings Dec 2023

Docai, Riley Badnin, Justin Brunings

Computer Science and Software Engineering

DocAI presents a user-friendly platform for recording, transcribing, summarizing, and classifying doctor-patient consultations. The application utilizes AssemblyAI for conversational transcription, and the user interface allows users to either live-record consultations or upload an existing MP3 file. The classification process, powered by 'ml-classify-text,' organizes the consultation transcription into SOAP (Subjective, Objective, Assessment, and Plan) format – a widely used method of documentation for healthcare providers. The result of this development is a simple yet effective interface that effectively plays the role of a medical scribe. However, the application is still facing challenges of inconsistent summarization from the AssemblyAI backend. Future work …


Independent- Dec. 1, 2023, Leslie Lozada Dec 2023

Independent- Dec. 1, 2023, Leslie Lozada

The Independent (1988-present)

No abstract provided.


A Model Of Immediate Implant Placement To Evaluate Early Osseointegration In 129/Sv Diabetic Mice, Cláudia Cristina Biguetti, Alexandra Arteaga, Bhuvana Lakkasetter Chandrashekar, Evelin Rios, Ryan Margolis, Danieli C. Rodrigues Dec 2023

A Model Of Immediate Implant Placement To Evaluate Early Osseointegration In 129/Sv Diabetic Mice, Cláudia Cristina Biguetti, Alexandra Arteaga, Bhuvana Lakkasetter Chandrashekar, Evelin Rios, Ryan Margolis, Danieli C. Rodrigues

School of Podiatric Medicine Publications

Purpose: To analyze the process of early oral osseointegration of titanium (Ti) implants in diabetic 129/Sv mice through microCT and histologic and immunohistochemical analysis.

Materials and methods: A group of 30 male 129/Sv mice was equally subdivided into two groups: (1) nondiabetic (ND), in which mice did not undergo systemic alterations and received a standard diet, and (2) diabetic (D), in which mice were provided a high-fat diet from the age of 6 weeks until the conclusion of the study and received two intraperitoneal (IP) injections of streptozotocin (STZ) at a concentration of 100 mg/Kg each. Each mouse underwent extraction …


Community-Based Cultivation Of Medicinal Plants: Preserving Florida's Native Plants, Shayna Dimmer Dec 2023

Community-Based Cultivation Of Medicinal Plants: Preserving Florida's Native Plants, Shayna Dimmer

USF St. Petersburg campus Honors Program Theses (Undergraduate)

This thesis explores the role of community-based cultivation of native medicinal plants as a strategy for promoting conservation, cultural awareness, and public health in Florida. Through a review of ethnobotanical literature and a hands-on case study conducted in a St. Petersburg community garden, the research documents the process of identifying, sourcing, propagating, and integrating two Florida native medicinal species, Passiflora incarnata (purple passionflower) and Callicarpa americana (American beautyberry), into a community setting. The study examines the historical and contemporary significance of medicinal plants, the challenges posed by overharvesting and habitat loss, and the growing need for sustainable cultivation practices. Findings …


They Need Rappers Like Me: A Poetic Analysis Of Identity And Disturbance In Women’S Rap Music, Willard Justice Dec 2023

They Need Rappers Like Me: A Poetic Analysis Of Identity And Disturbance In Women’S Rap Music, Willard Justice

Honors College

This thesis explores the theme of identity in rap music produced by Black women in the past five years (2017-2023). Hip hop feminism's theoretical underpinnings and viability are first established, then the impact that Black women rappers have on their listeners is encapsulated in a statement of significance. After establishing those ideas as a framework, ten songs by three main performers are analyzed for their use of disrespectability politics in developing or protecting the identity of the author. Each of these songs adds the author's voice to the conversation. Disrespectability politics owes much to Onika Maraj, who laid the groundwork …


Enhancing Urban Water Quality Through Biological-Chemical Treatment: Aquatic Macroinvertebrate Community And Temporal Chlorophyll-A Response, Matthew Chaffee Dec 2023

Enhancing Urban Water Quality Through Biological-Chemical Treatment: Aquatic Macroinvertebrate Community And Temporal Chlorophyll-A Response, Matthew Chaffee

Department of Agricultural and Biological Systems Engineering: Dissertations, Theses, and Student Research

With a growing human population, urbanization is impeding a plethora of natural waterways. Of these, urban ponds play a vital role in nutrient sequestration, flood prevention, and habitat sanctuaries. However, nutrient loading can reduce habitat effectiveness and promote harmful algae blooms. To reduce internal nutrient loads, a biological-chemical treatment strategy consisting of floating treatment wetlands (FTWs) and lanthanum were applied to two urban retention ponds, Densmore and Wilderness Ridge Ponds. To measure effectiveness, chlorophyll-a samples were collected and correlated with Sentinel-2. A novel band algorithm termed 3BR1 produced a strong correlation (R2 = 0.72) to physical chlorophyll-a …


Renovating The Ipmu Via Internet Of Things For Pollutant Emission Estimations In Poultry Facilities, Joshua Dotto Dec 2023

Renovating The Ipmu Via Internet Of Things For Pollutant Emission Estimations In Poultry Facilities, Joshua Dotto

Department of Agricultural and Biological Systems Engineering: Dissertations, Theses, and Student Research

The emissions of ammonia (NH3), particulate matter (PM2.5), and carbon dioxide (CO2) are major concerns in poultry facilities. They can pose environmental concerns and nuances. Robust and affordable measurement systems are needed to accurately measure in-barn concentrations and quantify the emissions.

The Intelligent Portable Monitoring Unit (iPMU or PMU3) developed in 2016 was reconstructed into PMU4 to include upgraded NH3 and PM2.5 sensors and wireless connectivity for a low-cost, robust, and accurate air quality monitoring device with contactless data transfer using the concept of Internet of Things (IoT). In addition, a user-friendly …


Developing Disinfection Strategies For Controlling Human Norovirus, Sars-Cov-2, And Clostridioides Difficile Endospores In Long-Term Care Facilities, Jinge Huang Dec 2023

Developing Disinfection Strategies For Controlling Human Norovirus, Sars-Cov-2, And Clostridioides Difficile Endospores In Long-Term Care Facilities, Jinge Huang

All Dissertations

Long-term care facilities (LTCFs) provide an environment favorable for the transmission of three critical human pathogens: human norovirus (HuNoV), severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), and Clostridioides difficile. Given residents in LTCFs are susceptible to infections due to their advanced ages and compromised immune systems, effective environmental surface disinfection plays a crucial role in controlling the spread of human pathogens within these settings and, therefore, mitigates the risk of infections caused by these pathogens. This dissertation aimed to assess the efficacy of various types of disinfectants against two HuNoV surrogates [feline calicivirus (FCV) and Tulane virus (TuV)], two …


Usage Statistics: Project Counter R5 Dr_D1 Report Fy2023 - Database Search And Item Usage, Andrée J. Rathemacher Dec 2023

Usage Statistics: Project Counter R5 Dr_D1 Report Fy2023 - Database Search And Item Usage, Andrée J. Rathemacher

Technical Services Reports and Statistics

Project COUNTER R5 Report DR_D1 for the University of Rhode Island for the period from July 1, 2022 - June 30, 2023. The DR_D1 report is defined as "Database Search and Item Usage." This report presents an annual total only and only includes those platforms successfully configured for automated harvesting via SUSHI.

File for download is Excel spreadsheet generated by Alma Analytics.

Results:

Searches Automated - 101,532

Searches Federated - 10,516

Searches Regular - 1,059,387

Total Item Investigations - 519,728

Total Item Requests - 219,645


Usage Statistics: Project Counter R5 Tr_B1 Report Fy2023 - Book Requests (Excluding Oa_Gold) By Title, Andrée J. Rathemacher Dec 2023

Usage Statistics: Project Counter R5 Tr_B1 Report Fy2023 - Book Requests (Excluding Oa_Gold) By Title, Andrée J. Rathemacher

Technical Services Reports and Statistics

Project COUNTER R5 Report TR_B1 for the University of Rhode Island for the period from July 1, 2022 - June 30, 2023. The TR_B1 report is defined as "Book Requests (Excluding OA_Gold)." This report presents an annual total only and only includes those platforms successfully configured for automated harvesting via SUSHI.

File for download is Excel spreadsheet generated by Alma Analytics.

Results:

Total Item Requests - 118,197

Unique Title Requests - 46,803


The New Comity Abstention, John Harland Giammatteo Dec 2023

The New Comity Abstention, John Harland Giammatteo

Journal Articles

In the past ten years, lower federal courts have quietly but regularly abstained from hearing federal claims challenging state court procedures, citing concerns of comity and federalism. Federal courts have dismissed a broad range of substantive challenges tasked to them by Congress, including under the Americans with Disabilities Act, the Indian Child Welfare Act, and various constitutional provisions, involving state court eviction proceedings, foster care determinations, bail and criminal justice policies, COVID-era safety practices, and other instances where state courts determine state policy.

This paper is the first to argue that these decisions constitute a new abstention doctrine, unmoored from …


Isotopic Ecology Of Bison And Bootherium At Big Bone Lick, Kentucky, Frances Stephenson Dec 2023

Isotopic Ecology Of Bison And Bootherium At Big Bone Lick, Kentucky, Frances Stephenson

Electronic Theses and Dissertations

Big Bone Lick (BBL) is a late Pleistocene and Holocene fossil locality in the Ohio River valley of Kentucky. This study utilized stable isotopes (δ13C, δ18O, and 87Sr/86Sr) in tooth enamel to reconstruct mobility and diet in Bison, as well as diet in Bootherium bombifrons. Isotopic results from seven Bison and two B. bombifrons are reported. Results suggest Pleistocene Bison and Bootherium occupied different dietary niches. Although both had C3-dominated diets, Bison occupied more open environments than B. bombifrons. Two bison from the late Holocene deposit had diets that consisted …


Improving Self-Regulation In First Graders, Areli Santiago Perez Dec 2023

Improving Self-Regulation In First Graders, Areli Santiago Perez

Capstone Projects and Master's Theses

This capstone project focuses on the development of self-regulation skills in a first grade class. Self-regulation is an individual's capacity to comprehend and control their behaviors, emotions, and thoughts (Bockmann & Yu, 2022). Self-regulation abilities influence a child's academic performance. For example, some school age children get easily distracted from one activity to another, struggle to follow directions, and find it difficult to regulate their feelings, which can seriously create difficulties in their learning capabilities. Therefore , there is a need for self-regulation strategies to be taught to young school age children. By improved emotional control, students with higher self-regulation …


Immune Evasion And Membrane Fusion Of Sars-Cov-2 Xbb Subvariants Eg51 And Xbb23, Julia N Faraone, Panke Qu, Negin Goodarzi, Yi-Min Zheng, Claire Carlin, Linda J Saif, Eugene M Oltz, Kai Xu, Daniel Jones, Richard J Gumina, Shan-Lu Liu Dec 2023

Immune Evasion And Membrane Fusion Of Sars-Cov-2 Xbb Subvariants Eg51 And Xbb23, Julia N Faraone, Panke Qu, Negin Goodarzi, Yi-Min Zheng, Claire Carlin, Linda J Saif, Eugene M Oltz, Kai Xu, Daniel Jones, Richard J Gumina, Shan-Lu Liu

Faculty, Staff and Student Publications

Immune evasion by SARS-CoV-2 paired with immune imprinting from monovalent mRNA vaccines has resulted in attenuated neutralizing antibody responses against Omicron subvariants. In this study, we characterized two new XBB variants rising in circulation - EG.5.1 and XBB.2.3, for their neutralization and syncytia formation. We determined the neutralizing antibody titers in sera of individuals that received a bivalent mRNA vaccine booster, BA.4/5-wave infection, or XBB.1.5-wave infection. Bivalent vaccination-induced antibodies neutralized ancestral D614G efficiently, but to a much less extent, two new EG.5.1 and XBB.2.3 variants. In fact, the enhanced neutralization escape of EG.5.1 appeared to be driven by its key …


A Transcriptomic Taxonomy Of Mouse Brain-Wide Spinal Projecting Neurons, Carla C Winter, Anne Jacobi, Junfeng Su, Leeyup Chung, Cindy T J Van Velthoven, Zizhen Yao, Changkyu Lee, Zicong Zhang, Shuguang Yu, Kun Gao, Geraldine Duque Salazar, Evgenii Kegeles, Yu Zhang, Makenzie C Tomihiro, Yiming Zhang, Zhiyun Yang, Junjie Zhu, Jing Tang, Xuan Song, Ryan J Donahue, Qing Wang, Delissa Mcmillen, Michael Kunst, Ning Wang, Kimberly A Smith, Gabriel E Romero, Michelle M Frank, Alexandra Krol, Riki Kawaguchi, Daniel H Geschwind, Guoping Feng, Lisa V Goodrich, Yuanyuan Liu, Bosiljka Tasic, Hongkui Zeng, Zhigang He Dec 2023

A Transcriptomic Taxonomy Of Mouse Brain-Wide Spinal Projecting Neurons, Carla C Winter, Anne Jacobi, Junfeng Su, Leeyup Chung, Cindy T J Van Velthoven, Zizhen Yao, Changkyu Lee, Zicong Zhang, Shuguang Yu, Kun Gao, Geraldine Duque Salazar, Evgenii Kegeles, Yu Zhang, Makenzie C Tomihiro, Yiming Zhang, Zhiyun Yang, Junjie Zhu, Jing Tang, Xuan Song, Ryan J Donahue, Qing Wang, Delissa Mcmillen, Michael Kunst, Ning Wang, Kimberly A Smith, Gabriel E Romero, Michelle M Frank, Alexandra Krol, Riki Kawaguchi, Daniel H Geschwind, Guoping Feng, Lisa V Goodrich, Yuanyuan Liu, Bosiljka Tasic, Hongkui Zeng, Zhigang He

Faculty, Staff and Student Publications

The brain controls nearly all bodily functions via spinal projecting neurons (SPNs) that carry command signals from the brain to the spinal cord. However, a comprehensive molecular characterization of brain-wide SPNs is still lacking. Here we transcriptionally profiled a total of 65,002 SPNs, identified 76 region-specific SPN types, and mapped these types into a companion atlas of the whole mouse brain1. This taxonomy reveals a three-component organization of SPNs: (1) molecularly homogeneous excitatory SPNs from the cortex, red nucleus and cerebellum with somatotopic spinal terminations suitable for point-to-point communication; (2) heterogeneous populations in the reticular formation with broad …


Single-Cell Analysis Of Chromatin Accessibility In The Adult Mouse Brain, Songpeng Zu, Yang Eric Li, Kangli Wang, Ethan J Armand, Sainath Mamde, Maria Luisa Amaral, Yuelai Wang, Andre Chu, Yang Xie, Michael Miller, Jie Xu, Zhaoning Wang, Kai Zhang, Bojing Jia, Xiaomeng Hou, Lin Lin, Qian Yang, Seoyeon Lee, Bin Li, Samantha Kuan, Hanqing Liu, Jingtian Zhou, Antonio Pinto-Duarte, Jacinta Lucero, Julia Osteen, Michael Nunn, Kimberly A Smith, Bosiljka Tasic, Zizhen Yao, Hongkui Zeng, Zihan Wang, Jingbo Shang, M Margarita Behrens, Joseph R Ecker, Allen Wang, Sebastian Preissl, Bing Ren Dec 2023

Single-Cell Analysis Of Chromatin Accessibility In The Adult Mouse Brain, Songpeng Zu, Yang Eric Li, Kangli Wang, Ethan J Armand, Sainath Mamde, Maria Luisa Amaral, Yuelai Wang, Andre Chu, Yang Xie, Michael Miller, Jie Xu, Zhaoning Wang, Kai Zhang, Bojing Jia, Xiaomeng Hou, Lin Lin, Qian Yang, Seoyeon Lee, Bin Li, Samantha Kuan, Hanqing Liu, Jingtian Zhou, Antonio Pinto-Duarte, Jacinta Lucero, Julia Osteen, Michael Nunn, Kimberly A Smith, Bosiljka Tasic, Zizhen Yao, Hongkui Zeng, Zihan Wang, Jingbo Shang, M Margarita Behrens, Joseph R Ecker, Allen Wang, Sebastian Preissl, Bing Ren

Faculty, Staff and Student Publications

Recent advances in single-cell technologies have led to the discovery of thousands of brain cell types; however, our understanding of the gene regulatory programs in these cell types is far from complete1-4. Here we report a comprehensive atlas of candidate cis-regulatory DNA elements (cCREs) in the adult mouse brain, generated by analysing chromatin accessibility in 2.3 million individual brain cells from 117 anatomical dissections. The atlas includes approximately 1 million cCREs and their chromatin accessibility across 1,482 distinct brain cell populations, adding over 446,000 cCREs to the most recent such annotation in the mouse genome. The mouse brain cCREs are …


Molecularly Defined And Spatially Resolved Cell Atlas Of The Whole Mouse Brain, Meng Zhang, Xingjie Pan, Won Jung, Aaron R Halpern, Stephen W Eichhorn, Zhiyun Lei, Limor Cohen, Kimberly A Smith, Bosiljka Tasic, Zizhen Yao, Hongkui Zeng, Xiaowei Zhuang Dec 2023

Molecularly Defined And Spatially Resolved Cell Atlas Of The Whole Mouse Brain, Meng Zhang, Xingjie Pan, Won Jung, Aaron R Halpern, Stephen W Eichhorn, Zhiyun Lei, Limor Cohen, Kimberly A Smith, Bosiljka Tasic, Zizhen Yao, Hongkui Zeng, Xiaowei Zhuang

Faculty, Staff and Student Publications

In mammalian brains, millions to billions of cells form complex interaction networks to enable a wide range of functions. The enormous diversity and intricate organization of cells have impeded our understanding of the molecular and cellular basis of brain function. Recent advances in spatially resolved single-cell transcriptomics have enabled systematic mapping of the spatial organization of molecularly defined cell types in complex tissues1-3, including several brain regions (for example, refs. 1-11). However, a comprehensive cell atlas of the whole brain is still missing. Here we imaged a panel of more than 1,100 genes in approximately 10 million cells across the …


Single-Cell Dna Methylome And 3d Multi-Omic Atlas Of The Adult Mouse Brain, Hanqing Liu, Qiurui Zeng, Jingtian Zhou, Anna Bartlett, Bang-An Wang, Peter Berube, Wei Tian, Mia Kenworthy, Jordan Altshul, Joseph R Nery, Huaming Chen, Rosa G Castanon, Songpeng Zu, Yang Eric Li, Jacinta Lucero, Julia K Osteen, Antonio Pinto-Duarte, Jasper Lee, Jon Rink, Silvia Cho, Nora Emerson, Michael Nunn, Carolyn O'Connor, Zhanghao Wu, Ion Stoica, Zizhen Yao, Kimberly A Smith, Bosiljka Tasic, Chongyuan Luo, Jesse R Dixon, Hongkui Zeng, Bing Ren, M Margarita Behrens, Joseph R Ecker Dec 2023

Single-Cell Dna Methylome And 3d Multi-Omic Atlas Of The Adult Mouse Brain, Hanqing Liu, Qiurui Zeng, Jingtian Zhou, Anna Bartlett, Bang-An Wang, Peter Berube, Wei Tian, Mia Kenworthy, Jordan Altshul, Joseph R Nery, Huaming Chen, Rosa G Castanon, Songpeng Zu, Yang Eric Li, Jacinta Lucero, Julia K Osteen, Antonio Pinto-Duarte, Jasper Lee, Jon Rink, Silvia Cho, Nora Emerson, Michael Nunn, Carolyn O'Connor, Zhanghao Wu, Ion Stoica, Zizhen Yao, Kimberly A Smith, Bosiljka Tasic, Chongyuan Luo, Jesse R Dixon, Hongkui Zeng, Bing Ren, M Margarita Behrens, Joseph R Ecker

Faculty, Staff and Student Publications

Cytosine DNA methylation is essential in brain development and is implicated in various neurological disorders. Understanding DNA methylation diversity across the entire brain in a spatial context is fundamental for a complete molecular atlas of brain cell types and their gene regulatory landscapes. Here we used single-nucleus methylome sequencing (snmC-seq3) and multi-omic sequencing (snm3C-seq)1 technologies to generate 301,626 methylomes and 176,003 chromatin conformation–methylome joint profiles from 117 dissected regions throughout the adult mouse brain. Using iterative clustering and integrating with companion whole-brain transcriptome and chromatin accessibility datasets, we constructed a methylation-based cell taxonomy with 4,673 cell groups and 274 …


Lessons For The Next Generation Of Scientists From The Second Annual Arthur And Sandra Irving Cancer Immunology Symposium, Christopher Alvarez-Breckenridge, Kristin G Anderson, Ana Luisa Correia, Shadmehr Demehri, Huy Q Dinh, Karen Olivia Dixon, Gavin P Dunn, Laura Evgin, Jeremy Goc, Zinaida Good, Nir Hacohen, Patrick Han, Pavel Hanč, John Hickey, Kelly Kersten, Beiyun C Liu, Aitziber Buque, Yuxuan 'Phoenix' Miao, J Justin Milner, Yuri Pritykin, Ferdinando Pucci, Nicole E Scharping, Lisa Sudmeier, Yufei Wang, Andreas Wieland, Michelle M Williams Dec 2023

Lessons For The Next Generation Of Scientists From The Second Annual Arthur And Sandra Irving Cancer Immunology Symposium, Christopher Alvarez-Breckenridge, Kristin G Anderson, Ana Luisa Correia, Shadmehr Demehri, Huy Q Dinh, Karen Olivia Dixon, Gavin P Dunn, Laura Evgin, Jeremy Goc, Zinaida Good, Nir Hacohen, Patrick Han, Pavel Hanč, John Hickey, Kelly Kersten, Beiyun C Liu, Aitziber Buque, Yuxuan 'Phoenix' Miao, J Justin Milner, Yuri Pritykin, Ferdinando Pucci, Nicole E Scharping, Lisa Sudmeier, Yufei Wang, Andreas Wieland, Michelle M Williams

Faculty, Staff and Student Publications

The Arthur and Sandra Irving Cancer Immunology Symposium has been created as a platform for established cancer immunologists to mentor trainees and young investigators as they launch their research career in the field. By sharing their different paths to success, the senior faculty mentors provide an invaluable resource to support the development of the next generation of leaders in the cancer immunology community. This Commentary describes some of the key topics that were discussed during the 2022 symposium: scientific and career trajectory, leadership, mentoring, collaborations, and publishing. For each of these topics, established investigators discussed the elements that facilitate success …


Aging Differentially Affects Axonal Autophagosome Formation And Maturation, Heather Tsong, Erika Lf Holzbaur, Andrea Kh Stavoe Dec 2023

Aging Differentially Affects Axonal Autophagosome Formation And Maturation, Heather Tsong, Erika Lf Holzbaur, Andrea Kh Stavoe

Faculty, Staff and Student Publications

Misregulation of neuronal macroautophagy/autophagy has been implicated in age-related neurodegenerative diseases. We compared autophagosome formation and maturation in primary murine neurons during development and through aging to elucidate how aging affects neuronal autophagy. We observed an age-related decrease in the rate of autophagosome formation leading to a significant decrease in the density of autophagosomes along the axon. Next, we identified a surprising increase in the maturation of autophagic vesicles in neurons from aged mice. While we did not detect notable changes in endolysosomal content in the distal axon during early aging, we did observe a significant loss of acidified vesicles …


Plastic, It's What's For Dinner: A Preliminary Comparison Of Ingested Particles In Bottlenose Dolphins And Their Prey, Leslie B Hart, Miranda Dziobak, Randall S Wells, Elizabeth Berens Mccabe, Eric Conger, Tita Curtin, Maggie Knight, John Weinstein Dec 2023

Plastic, It's What's For Dinner: A Preliminary Comparison Of Ingested Particles In Bottlenose Dolphins And Their Prey, Leslie B Hart, Miranda Dziobak, Randall S Wells, Elizabeth Berens Mccabe, Eric Conger, Tita Curtin, Maggie Knight, John Weinstein

Faculty, Staff and Student Publications

Microplastic ingestion was reported for common bottlenose dolphins (Tursiops truncatus) inhabiting Sarasota Bay, FL, USA, a community that also has prevalent exposure to plasticizers (i.e., phthalates) at concentrations higher than human reference populations. Exposure sources are currently unknown, but plastic-contaminated prey could be a vector. To explore the potential for trophic exposure, prey fish muscle and gastrointestinal tract (GIT) tissues and contents were screened for suspected microplastics, and particle properties (e.g., color, shape, surface texture) were compared with those observed in gastric samples from free-ranging dolphins. Twenty-nine fish across four species (hardhead catfish, Ariopsis felis; pigfish, Orthopristis …