Digital Commons Network^™

Bisacodyl Limits Chikungunya Virus In Vitro And Is Broadly Antiviral, Natalie June Lomascolo

Master's Theses

Identifying novel antivirals requires significant time and resource investment, and the continuous threat of viruses to human health necessitates commitment to antiviral identification and development. Developing antivirals requires years of research and validation, and recent outbreaks have highlighted the need for preparedness in counteracting pandemics. One way to facilitate development is to repurpose molecules already used clinically. By screening such compounds, we can accelerate antiviral development. Here, we screened compounds from the National Institutes of Health’s Developmental Therapeutic Program for activity against chikungunya virus, an alphavirus that is responsible for a significant outbreak in the Americas in 2013. Using this …

Elucidating The Function Of An Evolutionarily Conserved Col2Α1 Isoform During Vertebrate Development, Charles Lantz H. Ingersoll

Master's Theses

ABSTRACT

Formation of skeletal structural elements during vertebrate embryogenesis is largely a

conserved evolutionary process that employs numerous conserved genes and pathways. One

critical gene in this process is the highly conserved vertebrate Type II Collagen alpha 1 (col2α1).

It produces multiple splice isoforms that have different functions and expression patterns during

skeletogenesis. Two of the primary isoforms are of great interest; variant 1, the primary

embryonic isoform, and variant 2, a post embryonic isoform. While the more commonly known

post-embryonic isoform has been well studied over the last 30 years, identification of a unique

role for the evolutionarily conserved …

Development And Characterization Of A Tissue-Specific Reporter Line For Monitoring Circadian Clock Transcriptional Activity, Lilyan Mather

Master's Theses

Daily rhythms in physiology and behavior are produced by a circadian system consisting of a central clock located in the brain and peripheral clocks located in various peripheral tissues. Circadian clocks track time of day through the presence of a molecular clock that functions as a transcriptional-translational feedback loop. In the nucleus, the transcription factors CLOCK (CLK) and CYCLE (CYC) activate transcription of clock genes period (per) and timeless (tim). PER and TIM proteins slowly accumulate in the cytoplasm before entering the nucleus, where they inhibit CLK and CYC, and therefore repress their own transcription. Degradation of nuclear PER and …

A Process Evaluation Of Aunt Mary’S Storybook: Seeking To Improve The Relationship Between Incarcerated Parents And Their Children Through Literacy, Scott Charles Mcwilliams

Master's Theses

In response to the rise of incarceration in jails and prisons, and the number of children adversely impacted by their parents’ incarceration, in 1993, a nonprofit organized called Companions Journeying Together (“CJT”), created the Aunt Mary’s Storybook (AMS) program to foster positive connections between incarcerated parents to their children. What began as a Christmastime program for mothers in the Cook County Jail has grown into a year-round program operating in prisons and jails throughout Illinois. AMS provides those incarcerated with an opportunity to record themselves reading a book to their children, and AMS then mails/transmits the recording along with a …

Influences Of Latino Caregivers’ Input And Acculturation On Children’S Bilingual Development: A Speech Sample Analysis, Jordan Sierra Perry

Master's Theses

Interactions with caregivers are important for children’s development. In particular, the language input that young dual language learners (DLLs) receive from their primary caregivers affects their cultural knowledge and their bilingual language outcomes. However, relatively little research has been conducted to investigate the cultural contexts that influence Latino caregivers’ bilingual language use at home, including their acculturation level. Thus, the present study examined the relation between caregivers’ acculturation and their children’s bilingual (Spanish and English) language use, and whether this relation varied as a function of caregivers’ bilingual language use. Parent-report measures of bilingual language use, in addition to video …

Investigating The Role Of Nagc Reveals It To Be A Global Regulator, Natasha Peterson

Master's Theses

Bacterial colonization of host surfaces can result in symbiotic or pathogenic associations.As bacteria becomes more resistant to antibiotics, pathogenic bacterial colonization poses an increasing risk to human health. Symbiotic associations that have beneficial outcomes for both the host and the microbe can serve as models to investigate how bacterial processes affect colonization of host surfaces. The symbiotic relationship between the Gram-negative, bioluminescent bacterium Vibrio fischeri and the Hawaiian bobtail squid is one such model (E. V. Stabb & Visick, 2013). The study of this exclusive relationship has developed in a way where in vitro phenotypes can predict the ability of …

Positive Affect Facilitates Reduction In Depression Symptoms During A Mobile Mindfulness-Based Intervention, Andrew Rauch

Master's Theses

Depression negatively impacts the lives of many, and the rates are continuing to rapidly increase. Identifying patterns of modifiable affective psychological mechanisms that contribute to reducing depression symptoms is critical to improve the effectiveness of wellbeing interventions for individuals with depressive disorders. Depression has been characterized by valanced patterns of low positive affect and high negative affect, yet the extent to which these relative patterns of affectivity change in response to intervention remains less clear. The present study evaluated affective patterns in college students (n = 127) with elevated depression symptoms who participated in a randomized controlled trial (RCT) of …

How Black Are You?: The Influence Of Racial Centrality On Stereotype Threat In The Courtroom, Kendall Cathleen Redwood

Master's Theses

The current study explores whether stereotype threat, or fear of confirming negativestereotypes about one’s in-group, might manifest in the criminal justice system. This study specifically asks whether the threat of confirming stereotypes connecting race and crime manifest, among Black defendants, in nonverbal behaviors that might be perceived by observers as guilt. This research further explores whether racial centrality, the degree to which one identifies with one’s race, moderates effects of stereotype threat. Black female-identifying college students, who rated their degree of racial centrality, were randomly assigned to experience the activation of the race-crime stereotype or to a control condition. They …

The Role Of Identification In Black Individuals' Goals To Challenge Racism Through Confrontation And Collective Action, Efrata Sasahulih

Master's Theses

Anti-Black racism continues to harm Black individuals in the United States and throughout the world (e.g., police brutality, medical racism: Hoffman, Trawalter, Axt, & Oliver, 2016; Smiley & Fakunle, 2016; Stuber, Meyer, & Link, 2008). Psychological research has investigated the roots of prejudice and strategies to mitigate its negative consequences, including confrontation and collective action. However, research in this area has largely focused on ways that these strategies pose additional risk for targets of prejudice. This research determined if Black people who endorse individual-benefiting or group-benefiting goals following an instance of anti-Black racism use confrontation and collective action, respectively, to …

Comparison Of Feeding Functional Morphology Development In North American Esocids, Cheryl Theile

Master's Theses

Esocidae is a family of freshwater fishes within order Esociformes known for its long, cylindrical bodies, duck-bill snouts, and an impressive number of sharp teeth. There are four species, including two subspecies, in North America with areas of overlap of their geographic distribution. These ambush predators change diets from invertebrate prey to vertebrate prey during their first year of life, and the timing of this shift varies between species. In particular, the sister species Esox lucius (Northern Pike) and Esox masquinongy (Muskellunge) overlap in the upper Midwest, but E. masquinongy transitions to a vertebrate diet sooner in its development than …

Delayed Testosterone Treatment In A Rodent Model Of Repeat Mild Traumatic Brain Injury Attenuates Mechanisms Of Cellular Death In The Vestibular Nucleus, Michael Volyanyuk

Master's Theses

A common complaint after repetitive mild traumatic brain injury (rmTBI) is vestibular dysfunction, which can become persistent and decrease quality of life. Delayed testosterone treatment has been shown to reduce vestibular impairment and improve neuronal survival in the vestibular nucleus following rmTBI; however, the long-term molecular pathways associated with rmTBI and testosterone treatment remain widely unexplored. We utilized a 5-hit closed-head rodent rmTBI model and administered testosterone 35 days after injury (DPI). The vestibular nuclei were removed and prepared for RT-qPCR and western blotting. Gene expression and protein levels were elevated for markers of oxidative stress (NADPH oxidase 4) and …

Calcium-Mediated Induction Of Vibrio Fischeri Es114 Biofilms, Katia Elizabeth Semenchuk

Master's Theses

Vibrio fischeri bacteria form biofilms that facilitate symbiotic colonization of the Hawaiian bobtail squid, Euprymna scolopes. These host-associated biofilms require production of the SYP polysaccharide. However, it has been difficult to observe robust biofilm formation in vitro in a laboratory setting using wild-type strain ES114; instead, most work investigating syp-dependent biofilm formation has relied on genetically altered strains. Guided by recent findings that increasing calcium induces c-di-GMP (Tischler et al., 2021), a molecule known in other systems to promote biofilm formation, the question was posed as to whether high levels of calcium alone could promote syp-dependent biofilm formation by ES114. …

Knowledge, Experiences And Perceptions Of Abagusii Community Of Kisii County, Kenya On The Health Consequences Of Female Genital Mutilation (Fgm), Roselyter Riang’A, Moses Mutiso

Population Health, East Africa

Background: Health education is among the prominent forms of interventions that are used to advocate for eradication of the practice of FGM. However, little is known on affected communities’ actual experiences, knowledge and perceptions on these health education messages hence need for this study.

Objective: To understand Abagusii people’s knowledge, experiences and perceptions on the health consequences of FGM.

Methods: Data was collected from Abagusii community of Kisii County in Kenya using interview schedules, focus group discussions, key informants and document analysis. The collected data were qualitatively and quantitatively analysed using SPSS and MAXQDA programs

Results: …

A Case Report Of Papulo-Pustular Rosacea-Like Reaction After The Second Dose Of Pfizer Vaccine, Hayder Al-Hamamy, Zainab Jawad

Iraqi Postgraduate Medical Journal

Coronavirus disease-2019 (COVID-19) has affected countries around the world. The introduction of COVID-19 vaccines has proved the most effective weapon in the fight against the disease. The massive vaccination campaign against COVID-19 pandemic started in 2020 lead to significant decrease in the number of symptomatic infected patients and a decline in the mortality related to COVID-19 infection. However, with the vaccination of billions of people, data on vaccine-induced adverse reactions are also emerging. Here we report a 50 year old woman who manifested a papule-pustular rash within 24hours of receiving the second dose of pfizerBioNTech COVID19 vaccine. We report this …

Design And Synthesis Of Curcumin Analogs For Anticancer Activity And Discovery Of Novel Hit Molecules Targeting Cxcr4., Maryam Sadat Hosseini Zare

Dissertations (2016-Present)

Curcumin as a natural compound is made of various components including protein, carbohydrate, and curcuminoid. Curcuminoid is made of curcumin, desmethoxycurcumin and bis desmethoxycurcumin. Curcumin is used for the treatment of cancer and inflammatory disorders, but it has some therapeutic problems like poor bioavailability, poor efficacy, and chemical instability. To overcome these problems, the objective of this study is (1) synthesis of pyrazole curcumin analogs, (2) synthesis of triazole curcumin analogs, and (3) in-vitro study of the anticancer activity of these curcumin analogs on head & neck, breast, pancreatic and glioblastoma cancer cells. During this part of my Ph.D. project, …

Physical Activity And Sedentary Behaviour Among Children And Adolescents With Intellectual Disabilities During The Covid-19 Lockdown In China, Y. Q. Yuan, J. N. Ding, N. Bi, M. J. Wang, S. C. Zhou, X. L. Wang, S. H. Zhang, Y. Liu, G. Roswal

Research, Publications & Creative Work

Background: In the wake of the COVID-19 pandemic, preliminary research has reported a significant decline in physical activity (PA) and an increase in sedentary behaviour (SB) among typically developed children and adolescents. Limited research has looked at the current situation of PA and SB during this pandemic among children and adolescents with intellectual disabilities (ID). This study investigated the situations about PA and SB among school-aged children and adolescents with ID on China’s mainland during the COVID-19 outbreak.

Methods: In total, 837 parents of children and adolescents (ages 6–18 years) with ID from 15 special education schools of Shandong Province …

The Other In The Self: The Ontological Metaphoricity Of Language, Cristina Valencia Mazzanti, Melissa Freeman

Education and Child Study: Faculty Publications

Through text and illustrations, I (first author) theorize language as a being that becomes and revels in its plurality, illuminating the inherent and necessary aesthetic and multimodal dimensions of any struggle toward meaning. To do so, I start by foregrounding my own experiences with the diversity that characterizes language to portray the significance of studying its ontology and plurality. I then identify philosophical hermeneutics as a practical metatheory that centrally positions language as a medium for understanding. From a hermeneutic perspective, language manifests as a network of meaning that is constituted through an ontological metaphoricity that echoes a capacity to …

Twitter: More Than Tweets For Undergraduate Student Researchers, Nathan S. Reyna, Christin Pruett, Mike Morrison, Jennifer Fowler, Sumali Pandey, Lori Hensley

Research, Publications & Creative Work

A vital component of a CURE is feeling a sense of community. While our students often present to each other at the end of the semester, we have struggled with having them interact with a wider audience. A few students may present class results at a regional or national meeting; however, feasibility limits this opportunity to only a handful of students. Twitter has been used as a tool to disseminate scientific findings (1, 2). As a result of COVID-19, scientific societies have used Twitter to provide snapshots of research to be presented at their virtual meetings using “Twitter Posters” (Michael …

Biofilms On Plastic Litter: Community Composition And Activity And The Effects On Ecosystem Processes, Raul F. Lazcano

Master's Theses

The rapid increase of plastic production and disposal has resulted in plastic pollution becoming a global problem. In aquatic ecosystems, plastic litter is a substrate for biofilms, but little research has simultaneously assessed the effects of plastic litter on biofilm activity, community composition, and ecosystem processes. Our objectives were to: (1) measure biofilm activity and community composition on plastic litter relative to a natural surface in an urban river and (2) assess the impact of microplastic pollution on ecosystem metabolism and N2 flux in an oligotrophic lake. For objective (1) we incubated three common plastics with distinct physical and chemical …

The World Health Organization, The Trump Administration, American Public Opinion, And China: A Principal-Agent Problem, Megan Patrice Larson

Master's Theses

Why did the United States perceive the World Health Organization as ineffective during the COVID-19 pandemic? To answer this question, this research delves into current principal-agent model literature to reproduce mechanisms present by the WHO and the United States. Current research fails to explain the WHO's effectiveness. By borrowing from realist theory and political psychology and analyzing various declarations made by the United States, this analysis found preliminary evidence that the idea of WHO's ineffectiveness was primarily shaped by the Trump Administration and American public opinion. Moreover, because of the disinformation and misinformation spread by the Trump Administration, the WHO …

Dna Methylation Patterns Of Line-1 Elements Specific To Hc21p In Cancer Cells, Dametria Jetters

Master's Theses

Long Interspersed Nuclear Elements (LINEs) are retrotransposons. They function by making a cDNA copy, reverse-transcribing it into DNA and reinserting itself back into the genome; thereby, increasing its copy number. Sequencing of the human genome revealed that LINEs (L1) are underrepresented on the short arm of human chromosome 21 (HC21), but it is enriched in full-length copies. A number of previous studies have investigated the DNA methylation patterns of L1s in cancer cells, but these studies are typically done in euchromatic regions. The heterochromatic short arm of HC21 is primarily composed of heterochromatin. However these regions can be targeted for …

Unveiling Bacterial Hitchhikers In The Urobiome, Genevieve Baddoo

Master's Theses

Microbial communities of bacteria, viruses, and fungi form complex interactions in nature. However, our understanding of these communities is limited to those that can be cultured in the lab. Enhanced culture methods have enabled the isolation of numerous fastidious species from urinary tract, definitively proving that the urinary tract of asymptomatic individuals is not sterile. In working with these isolates, our group has observed several instances in which a “purified” isolate actually harbors more than one species. We refer to these additional members as bacterial hitchhikers, a term previously used to describe similar occurrences in soil communities. I hypothesized that …

Modeling The Impact Of Peer Mentorship To Support First-Year Student Well-Being, Sarah Broner

Master's Theses

The transition to college is characterized by instability in a variety of life domains, with some students experiencing sharp declines in well-being across the first year of college (Conley et al., 2014). Well-established in the literature is the link between this life transition and increased rates of mental illness, including depression, anxiety, stress, and relationship problems (Auerbach et al., 2018). The adjustment to higher education can be particularly difficult for underserved students (Carter et al., 2013; Kalsner & Pistole, 2003), who may not have access to the same types of knowledge, resources, and privileges as their White counterparts on campus. …

Hispanic Teachers And Leaders In Catholic Schools: Special Issue Introduction, Melodie Wyttenbach, Hosffman Ospino

Journal of Catholic Education

Introduction to the Special Issue

The Impact Of Perceived Discrimination And Stigma Consciousness On Connectedness, Trust, And Satisfaction In Healthcare Domains During The Covid-19 Pandemic, Devon Richardson

Master's Theses

This study will assess the relations between perceived experiences of discrimination within healthcare settings and connectedness, trust, and satisfaction with services in healthcare domains among African Americans. It will also examine if the Covid-19 pandemic has exacerbated this relationship. I predict that African Americans in the COVID-19 discrimination condition (versus the control condition) will report lower connectedness and trust with healthcare workers and lower satisfaction with healthcare services. I also predict that stigma consciousness will moderate this effect. For African Americans reporting lower levels of stigma consciousness, I expect there will be a negative relation between perceiving discrimination and connectedness, …

Psychological Symptoms, Visceral Pain Sensitivity, And Observations Of Parent Pain Behavior Are Related To Somatic Symptoms In Premenarchal Adolescents, Genevieve Roth

Master's Theses

Somatic symptoms are a risk factor for the development of chronic pelvic pain in women. The present study characterized somatic symptoms and their relations with psychological, pain sensitivity, and parental factors in a community sample of premenarchal adolescents, which marks an initial step in advancing the identification of potential modifiable factors related to menstrual pain and chronic pain development. Pain sensitivity (visceral and bodily factors) and psychological symptoms (anxiety and stress) were predicted to be related to somatic symptoms (e.g, widespread bodily discomfort) in premenarchal adolescents. Additionally, adolescents’ observation of parent pain behavior was expected to be specifically associated with …

Book Review: Teacher Diversity And Student Success: Why Racial Representation Matters In The Classroom, Aubrey Scheopner Torres

Journal of Catholic Education

No abstract is published with book reviews

Rethinking The Hispanic Teacher Shortage: Dual Language Schools As Identity-Affirming Organizations, Elena Sada, Katie Ward

Journal of Catholic Education

Research has established the connection between the academic success of culturally and linguistically diverse students, and their schools’ ability to recruit and retain teachers that reflect such diversity (Shirrell et al., 2019). Studies have also highlighted the criticality of the students’ home language use as a way to enhance academic growth and develop their sociocultural competence and well-being (Feinauer & Howard, 2014). There is no research, however, addressing the differences between the experiences of Hispanic teachers in bilingual Catholic education compared to those in monolingual English Catholic education. This article highlights the differences captured by a recent study, and discusses …

The Influence Of Shoe And Cleat Type On Lower Extremity Muscle Activation In Youth Baseball Pitchers, Jacob R. Gdovin, Chip Wade, Lauren A. Luginsland, Charles C. Williams, Riley Galloway, John C. Garner

Human Movement Studies & Special Education Faculty Publications

Background: Baseball pitching is a dynamic movement where the lower extremities generate and sequentially transfer energy to the upper extremities to maximize ball velocity. The need for lower body muscular strength to produce adequate push-off and landing forces has been documented; however, the influence footwear and surface inclination has on muscle activation remains unknown.

Objectives: Determine how pitching in molded cleats and turf shoes from a pitching mound and flat ground affects stride-leg muscle activation in youth baseball pitchers while determining percent activation during each pitching phase.

Methods: Cross – sectional study analyzing mean muscle activity and percent activation of …

Longitudinal Changes Of Cardiorespiratory Fitness Performance In High School: Association With Individual And School-Based Variables, Xihe Zhu, Justin A. Haegele, Jinting Shao, Summer Davis

Human Movement Studies & Special Education Faculty Publications

This study aimed to model adolescents’ cardiorespiratory fitness performance change trajectories longitudinally across high school years and its relation to school- and individual/student-level factors. We employed hierarchical linear modeling to examine longitudinal cardiorespiratory fitness performance changes, as measured by the progressive aerobic capacity endurance run (PACER), over the years, between sexes, and in association with the school-level variables. Participants were 76,227 adolescents from 80 high schools in the mid-Atlantic region of the United States. School-level academic performance (SAP), the percent of students eligible for free and reduced-price meals (FARM), and physical education student-faculty ratio were obtained with permission from the …