Digital Commons Network^™

The Prostate Cancer Androgen Receptor Cistrome In African American Men Associates With Upregulation Of Lipid Metabolism And Immune Response, Jacob E Berchuck, Elio Adib, Sarah Abou Alaiwi, Amit K Dash, Jin Na Shin, Dallin Lowder, Collin Mccoll, Patricia Castro, Ryan Carelli, Elisa Benedetti, Jenny Deng, Matthew Robertson, Sylvan C Baca, Connor Bell, Heather M Mcclure, Talal El Zarif, Matthew P Davidsohn, Gitanjali Lakshminarayanan, Kinza Rizwan, Darlene G Skapura, Sandra L Grimm, Christel M Davis, Erik A Ehli, Kaitlin M Kelleher, Ji-Heui Seo, Nicholas Mitsiades, Cristian Coarfa, Mark M Pomerantz, Massimo Loda, Michael Ittmann, Matthew L Freedman, Salma Kaochar

Faculty, Staff and Students Publications

African-American (AA) men are more likely to be diagnosed with and die from prostate cancer than European American (EA) men. Despite the central role of the androgen receptor (AR) transcription factor in prostate cancer, little is known about the contribution of epigenetics to observed racial disparities. We performed AR chromatin immunoprecipitation sequencing on primary prostate tumors from AA and EA men, finding that sites with greater AR binding intensity in AA relative to EA prostate cancer are enriched for lipid metabolism and immune response genes. Integration with transcriptomic and metabolomic data demonstrated coinciding upregulation of lipid metabolism gene expression and …

A Single-Cell Transcriptional Gradient In Human Cutaneous Memory T Cells Restricts Th17/Tc17 Identity, Christopher P Cook, Mark Taylor, Yale Liu, Ralf Schmidt, Andrew Sedgewick, Esther Kim, Ashley Hailer, Jeffrey P North, Paymann Harirchian, Hao Wang, Sakeen W Kashem, Yanhong Shou, Timothy C Mccalmont, Stephen C Benz, Jaehyuk Choi, Elizabeth Purdom, Alexander Marson, Silvia B V Ramos, Jeffrey B Cheng, Raymond J Cho

Staff and Researcher Publications

The homeostatic mechanisms that fail to restrain chronic tissue inflammation in diseases, such as psoriasis vulgaris, remain incompletely understood. We profiled transcriptomes and epitopes of single psoriatic and normal skin-resident T cells, revealing a gradated transcriptional program of coordinately regulated inflammation-suppressive genes. This program, which is sharply suppressed in lesional skin, strikingly restricts Th17/Tc17 cytokine and other inflammatory mediators on the single-cell level. CRISPR-based deactivation of two core components of this inflammation-suppressive program, ZFP36L2 and ZFP36, replicates the interleukin-17A (IL-17A), granulocyte macrophage-colony-stimulating factor (GM-CSF), and interferon gamma (IFNγ) elevation in psoriatic memory T cells deficient in these transcripts, functionally validating …

Poziotinib Inhibits Her2-Mutant-Driven Therapeutic Resistance And Multiorgan Metastasis In Breast Cancer, Rashi Kalra, Ching Hui Chen, Junkai Wang, Ahmad Bin Salam, Lacey E Dobrolecki, Alaina Lewis, Christina Sallas, Clayton C Yates, Carolina Gutierrez, Balasubramanyam Karanam, Meenakshi Anurag, Bora Lim, Matthew J Ellis, Shyam M Kavuri

Faculty, Staff and Student Publications

The pan-HER tyrosine kinase inhibitor (TKI) neratinib is therapeutically active against metastatic breast cancers harboring activating HER2 mutations, but responses are variable and often not durable. Here we demonstrate that recurrent HER2 mutations have differential effects on endocrine therapy responsiveness, metastasis, and pan-HER TKI therapeutic sensitivity. The prevalence and prognostic significance may also depend on whether the HER2 mutant has arisen in the context of lobular versus ductal histology. The most highly recurrent HER2 mutant, L755S, was particularly resistant to neratinib but sensitive to the pan-HER TKI poziotinib, alone or in combination with fulvestrant. Poziotinib reduced tumor growth, diminished multiorgan …

Elucidating Factors Affecting Cercosporin Production In Cercospora Leaf Blight Of Soybean To Develop Better Tools For Disease Management, Maria Izabel Costa De Novaes

LSU Doctoral Dissertations

Fungal toxins are pathogenicity/virulence factors for many plant diseases, including Cercospora leaf blight (CLB) of soybean (Glycine max (L.) Merr.). The pathogen, Cercospora cf. flagellaris, produces a perylenequinone toxin, cercosporin, which is activated by light to produce damaging reactive oxygen species. To identify novel solutions for CLB management, we studied factors that may impact the biology of cercoporin: fungal lipid droplets (LDs) and mineral and trace element composition (ionome) of the host. Furthermore, since artificial inoculation with the pathogen has been challenging, we also explored the usefulness of a previously developed cercosporin-based assay to study disease resistance. LDs …

A Central Alarm System That Gates Multi-Sensory Innate Threat Cues To The Amygdala, Sukjae J Kang, Shijia Liu, Mao Ye, Dong-Il Kim, Gerald M Pao, Bryan A Copits, Benjamin Z Roberts, Kuo-Fen Lee, Michael R Bruchas, Sung Han

2020-Current year OA Pubs

Perception of threats is essential for survival. Previous findings suggest that parallel pathways independently relay innate threat signals from different sensory modalities to multiple brain areas, such as the midbrain and hypothalamus, for immediate avoidance. Yet little is known about whether and how multi-sensory innate threat cues are integrated and conveyed from each sensory modality to the amygdala, a critical brain area for threat perception and learning. Here, we report that neurons expressing calcitonin gene-related peptide (CGRP) in the parvocellular subparafascicular nucleus in the thalamus and external lateral parabrachial nucleus in the brainstem respond to multi-sensory threat cues from various …

Purification Of Artepillin C (Arc) With Anticancer Activities From Supercritical Co2-Ethanol Extracts Of Brazilian Green Propolis By Using Adsorption Column Chromatography, Lin-Hsiang Chuang, Wen-Chin Chen, Wen Pei, Ming-Hsi Chuang, Todd Hsu, Po-Chao Chuang

Journal of Marine Science and Technology–Taiwan

Phenolic compounds with varying phenolic structures are present in both terrestrial and marine organisms. Artepillin C (ARC) is a prenylated phenolic compound produced specifically in Brazilian green propolis. Earlier studies have demonstrated the potential of ARC as a dietary supplement for cancer prevention due to its ability to suppress tumor growth and metastasis. Here we report the development of an effective adsorption chromatography combined with supercritical CO2 extraction for the massive purification of ARC. A mixture of supercritical CO2 and ethanol extract of Brazilian green propolis was passed through a stainless chromatographic column packed with protruding metals at 4,000 psi …

A Stochastic Sailing Speed Optimization And Vessel Deployment Problem In Liner Shipping, Dung-Ying Lin, Pak Weng Leong

Journal of Marine Science and Technology–Taiwan

In this research, we consider the stochastic sailing speed optimization and vessel deployment problem and examine the trade-off between sailing speed and the number of vessels required to provide a certain service in international liner shipping. Solving this kind of stochastic program is a challenging task. To address this issue, we construct a mathematical formulation based on Ng (1) and Ng (2) and enhance the linearization techniques from Wang and Meng (3) so that problem instances of an even larger scale can be solved. The proposed formulation has been numerically applied to solve realistic cases, and the empirical results show …

Vehicle Assignment For Chemicals Transportation, Yu-Liang Liu, Juan Huang, Ching-Wu Chu

Journal of Marine Science and Technology–Taiwan

Vehicle assignment is a significant challenge faced by chemical logistics companies. The chemical industry typically outsources the delivery of chemicals to professional logistics companies to meet customers' complex and demanding needs. In this regard, this research explores the case study of a logistics company that selects its delivery modes and formulates its vehicle assignment plans based on its personnel’s experience rather than any formal system and plans its vehicle routes on the day before the delivery. This study seeks to improve the efficiency and immediacy of logistics companies’ vehicle assignments, understand vehicle route planning, maximize space utilization, and minimize transportation …

A Hybrid Finite Difference Method For Singularly Perturbed Delay Partial Differential Equations With Discontinuous Coefficient And Source, Nitika Sharma, Aditya Kaushik

Journal of Marine Science and Technology–Taiwan

The article presents a hybrid finite difference scheme to solve a singularly perturbed parabolic functional differential equation with discontinuous coefficient and source. The simultaneous presence of deviating argument with a discontinuous source and coefficient makes the problem stiff. The solution of the problem exhibits turning point behaviour across discontinuity as tends to zero. The hybrid scheme presented is a composition of a central difference scheme and a midpoint upwind scheme on a specially generated mesh. At the same time, an implicit finite difference method is used to discretize the time variable. Consistency, stability, and convergence of the presented numerical approach …

Simulating And Predicting Offshore Oil Spills By Using Cellular Automata, Lin Liu, Wanwu Li, Yumeng Cui, Guanghui Hu, Hang Li

Journal of Marine Science and Technology–Taiwan

In recent years, offshore oil spill accidents have caused substantial damage to both marine organisms and marine ecology. Accurate simulation and prediction of oil spills could provide a scientific basis for assessing losses due to oil spills and provide support for emergency decisions regarding oil spills. We analyzed the advantages and disadvantages of existing oil spill models and constructed an offshore oil spill model based on cellular automata (CA). By considering both the flow field and wind field, we formulated new CA rules for the diffusion and drift of a pollution zone, and in our model, the cellular state in …

Design And Testing Of A Bearing Less Piezo Jet Micro Heat Sink, Rong-Tsu Wang, Jung-Chang Wang, Sih-Li Chen

Journal of Marine Science and Technology–Taiwan

The present study utilized piezoelectric ceramics (PC) as actuators to design five new piezoelectric heatsinks and preliminarily investigated the different design types and their operating conditions, such as the frequency, placement distance, thickness between piezoelectric sheets, piezoelectric sheet size, and noise produced. The results showed that when the micro heatsink bearingless piezo jet was placed too close to the heat source, the high temperature sucked back the surrounding fluids, causing the fluid chamber temperature to rise and the cooling effect to be reduced. Therefore, the heatsink should be placed between 10 and 20 mm from the heat source. With the …

The Prep Act And The Countermeasures Injury Compensation Program: Past, Present, And Future, Allison M. Whelan

DePaul Law Review

No abstract provided.

Racial Inequality, Covid-19, And Health And Unemployment Insurance: Lessons Learned And Pathways Forward, Shauhin A. Talesh

DePaul Law Review

No abstract provided.

Regulating Health Care: Perspectives From Government Failure During The Covid-19 Pandemic, David A. Hyman, Charles Silver

DePaul Law Review

No abstract provided.

Research

Appalachia

No abstract provided.

Effects Of Varying Load Intensity On Skeletal Muscle Damage Between Two Isovolumic Resistance Exercise Bouts, Lee J. Winchester, Cody E. Morris, Patton Allen, Teresa Wiczynski, Scott W. Arnett, T. Scott Lyons

International Journal of Exercise Science

International Journal of Exercise Science 15(4): 1212-1221, 2022. There are limited data comparing the efficacy of resistance loads within the hypertrophy range for promoting muscular growth, particularly when similar training volumes are utilized. The purpose of this study was to determine if two similar volume-loads, utilizing different intensities, would produce dissimilar muscular damage and inflammation. Eleven resistance-trained, college-aged males participated in this study. After testing 1RM barbell squats, participants completed two similar volume-load barbell squat sessions at two different resistance loads (67% and 85% of 1RM) on two separate visits. Venous blood samples were collected at baseline and one hour …

Beyond ‘Rising Tides’ And ‘Lying Flat’: Emergent Cultural Practices Among Youth In Urban China, Diego Gullotta, Lili Lin

CLCWeb: Comparative Literature and Culture

In their article, “Beyond ‘Rising Tides’ and ‘Lying Flat’: Emergent Cultural Practices Among Youth in Urban China”, Diego Gullotta and Lili Lin examine how Chinese youth are positioned within the dominant culture, how young people appropriate space in their emergent cultural practices, and how they negotiate meaning-making. The article first analyses the rising tides (houlang) video, sponsored jointly by the state and the private sector, and argues that it reduces youth to a homogenous subject inscribed into the discourse of “China’s rise” (zhongguo jueqi) via emotional mobilization. The “lying flat” phenomenon represents young people’s negative response to …

Breadtube Rising: How Modern Creators Use Cultural Formats To Spread Countercultural Ideology, Jj Sylvia Iv, Kyle Moody

CLCWeb: Comparative Literature and Culture

In their article, “BreadTube Rising: How Modern Creators Use Cultural Formats to Spread Countercultural Ideology,” J.J. Sylvia IV and Kyle Moody analyze the rise of BreadTube. Scholars have argued that YouTube’s algorithms lead to greater radicalization (Ribeiro et al.) and bad actors have weaponized algorithms to draw users into conspiracies (boyd, What Hath We Wrought?). This article adds to this by linking these practices to the commodification of social media that spread misinformation as adaptations of socially and rhetorically mediated technologies. It analyzes how the economics of YouTube and other platforms demand that user-generated content fit within paradigms of …

Biopolitics In The Twenty-First Century: India And The Pandemic, _ Swatie, Rashee Mehra

CLCWeb: Comparative Literature and Culture

Swatie and Rashee Mehra discuss in their "Biopolitics in the Twenty-first Century: India and the Pandemic”, the rise of the biopolitical state in India in the 2020s. The article emphasizes the relevance of Michel Foucault’s work on biopolitics for the pandemic in India. The biopolitical governmentality of the Indian state operates at several levels to politicize ‘life itself’: racism (the notion that sections of the population are disposable), economics (the notion of privatization of care), and the logic of contagion (based on ideas of threat perception and risk). The article engages with biopolitics in the 21st century and looks at …

Kazuo Ishiguro And The Service Economy, Kate Montague

CLCWeb: Comparative Literature and Culture

In her article, “Kazuo Ishiguro and the Service Economy,” Kate Montague argues that Kazuo Ishiguro’s novels enact a poetics of work for the present moment—not just at the level of narrative but also in the kind of language used to describe the service economies his characters are doomed to inhabit. In his best-known novels, a clinical, bureaucratic, and even glorifying lexicon of “donations,” “completions,” “substitutions,” and “lifting” is betrayed by the reality of work grounded in horror. In Ishiguro’s worlds, which are very much our own, the out-sourcing of reproductive and domestic labor is enabled by a larger system in …

When The Queer East Met The American West: How The Same-Sex Relationship Between A Syrian “Princess” And Her “Secretary” Destabilized Orientalism In The Early Twentieth Century, Matthew Champagne

Madison Historical Review

No abstract provided.

Lesbian, Gay, And Bisexual Students Experiencing Homelessness And Substance Use In The School Context: A Statewide Study, Hadass Moore, Kris De Pedro

Education Faculty Articles and Research

PURPOSE

This study explored differences between lesbian, gay, and bisexual (LGB)-housed and homeless students regarding substance use patterns on and off school grounds and the unique contribution of homelessness to substance use in school.

METHODS

Data were from the 2013-2015 California Healthy Kids Survey, a statewide survey of school protective factors and risk behaviors. A representative sample of 9th- and 11th-grade students (N = 20,337) was used. Comparisons between housed (n = 19,456) and homeless (doubled up: n = 715; acute homeless: n = 166) LGB students were conducted. We used chi-square tests to compare rates of lifetime, past-30-day, and …

Investigating Features And Output Correlation Coefficient Of Natural Fiber-Reinforced Poly(Lactic Acid) Biocomposites, Andreas Federico, Siti Norasmah Surip, Wan Nor Raihan Wan Jaafar, Jaka Fajar Fatriansyah, Agrin Febrian Pradana

Journal of Materials Exploration and Findings

Polylactic acid (PLA) material has the potential to be applied in various industrial fields, but this material has shortcomings in terms of mechanical properties, especially mechanical strength, due to brittleness nature of PLA. The manufacture of PLA composite material with the addition of natural fibers as a reinforcing phase is one of the methods to increase the impact strength and maintain the biodegradable properties of the material. However, in theory, there are many factors that affect the mechanical properties of composite materials, thus making the mechanical properties of composites more complex than monolithic materials. The mechanical properties of these composite …

Teacher As Student: Matters Of Exploration And Radical Vulnerability In The Classroom, Elizabeth Giardina

Exploring How We Teach

• GSIs can find pedagogical value in demonstrating an exploratory learning process, and, in sharing the partialities that come with that process, we can find opportunities for our own intellectual development and well-being.
• Radical vulnerability is a powerful tool for building a community of responsibility and care.
• Acts of transparent vulnerability can reframe course content as relevant to students’ broader lives, while also modeling a method of community- and self-care.

Graduate Teaching Communities Of Practice: Fostering A Sense Of Belonging And Professional Development For Graduate Students, By Graduate Students, April Athnos, Tianyi Kou-Herrema, Matthew Langley, Emile Oshima, Harrison Parker, Hima Rawal, Olivia Wilkins, Alexandra Lee, Seth Hunt, Ellen Searle, Nathalie Marinho

Exploring How We Teach

• Communities of Practice provide explicit formal recognition for teaching work and serve as a network of pedagogical resources.
• Communities of Practice create a safe space and a strengthened sense of community.
• Communities of Practice can be formed anywhere to meet any set of needs but always thrive with members’ agency and institutional support.

Don't Forget To Breathe: Advice For Coping With Graduate Teaching Anxiety, Ryan Cheek

Exploring How We Teach

• Graduate teaching anxiety is a unique form of communication apprehension.
• Ignore your overactive amygdala. Teaching will not kill you.
• Teach with empath. Students are more afraid of you than you are of them.
• Don’t forget to breathe. A hospitable atmosphere is key to successful teaching.
• Institutions should better support their graduate student instructors.

Trends In Health Outcomes Before And After Long Term Care Federal Regulations Limited Antipsychotic Medication Use: A Secondary Data Analysis, Karen Robson

Electronic Theses and Dissertations

Trends in resident falls did not significantly change, although trends in serious injury from falls declined ten years after regulations restricted antipsychotic medications in long-term care facilities. Trends in functional and continence status worsened after the regulations were implemented.

Hexavalent Chromium Increases The Metabolism And Genotoxicity Of Aromatic Amine Carcinogens 4-Aminobiphenyl And Β-Naphthylamine In Immortalized Human Lung Epithelial Cells, James T.F. Wise, Raúl A. Salazar-González, Kennedy M. Walls, Mark A. Doll, Mariam R. Habil, David W. Hein

Faculty Publications

Humans are exposed to carcinogenic chemicals via occupational and environmental exposures. Common chemicals of concern that can occur in exposures together are aromatic amines (e.g., 4-aminobiphenyl [4-ABP] and β-naphthylamine [BNA]) and hexavalent chromium (Cr[VI]). Arylamine N-acetyltransferases 1 and 2 (NAT1 and NAT2) are key to the metabolism of aromatic amines and their genotoxicity. The effects of Cr(VI) on the metabolism of aromatic amines remains unknown as well as how it may affect their ensuing toxicity. The objective of the research presented here is to investigate the effects of Cr(VI) on the metabolism and genotoxicity of 4-ABP and BNA in immortalized …

Attributing Differences Of Solar-Induced Chlorophyll Fluorescence (Sif)-Gross Primary Production (Gpp) Relationships Between Two C4 Crops: Corn And Miscanthus, Genghong Wu, Kaiyu Guan, Chongya Jiang, Hyungsuk Kimm, Guofang Miao, Carl J. Bernacchi, Caitlin E. Moore, Elizabeth A. Ainsworth, Xi Yang, Joseph A. Berry, Christian Frankenberg, Min Chen

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

There remains limited information to characterize the solar-induced chlorophyll fluorescence (SIF)-gross primary production (GPP) relationship in C4 cropping systems. The annual C4 crop corn and perennial C4 crop miscanthus differ in phenology, canopy structure and leaf physiology. Investigating the SIF-GPP relationships in these species could deepen our understanding of SIF-GPP relationships within C4 crops. Using in situ canopy SIF and GPP measurements for both species along with leaf-level measurements, we found considerable differences in the SIF-GPP relationships between corn and miscanthus, with a stronger SIF-GPP relationship and higher slope of SIF-GPP observed in corn compared to miscanthus. These differences were …

The Impact Of Appearance On Transgender Victimization, Tabrina M. Bratton

Theses and Dissertations

Trans people experience disproportionate rates of victimization compared to cisgender people. This disparity persists despite the finding that many of the same predictors of cisgender victimization have been identified for transgender victimization (e.g., age, race, income, employment, sex work participation, homelessness), which suggest transgender-specific factors may be at play. Some scholars have proposed that transgender victimization is due to their non-conforming appearance. The purpose of this dissertation was to expand our understanding of anti-trans violence by concentrating on the relationship between appearance and sexual and recent physical victimization. This dissertation builds upon the existing transgender victimization literature by focusing on …