Digital Commons Network^™

Identification Of Secondary Structural Elements Contained Within The Intrinsically Disordered N-Terminal Tail Of The Bloom’S Syndrome Helicase., Vivek Somasundaram

USF Tampa Graduate Theses and Dissertations

The Bloom’s Syndrome Helicase (BLM) is one of five human RecQ helicases and is necessary for maintenance of genome stability. Whilst a crystal structure exists for the C-terminal domain of BLM, very limited structural knowledge is known about the intrinsically disordered N-terminal tail. This lack of insight exists, despite the fact that the N-terminus of BLM is essential for the overall biological activity of BLM. Here we provide an Nuclear Magnetic Resonance spectroscopy based approach that we used to identify two distinct ⍺-helices contained within the first 100 residues of BLM. In addition, we propose a mutagenesis-based approach involving rationally …

August 24, 2022, Faculty, Illinois Wesleyan University

2022-2023

No abstract provided.

Igg Antibodies To Sars-Cov-2 In Asymptomatic Blood Donors At Two Time Points In Karachi, Muhammad Hasan, Bushra Moiz, Shama Qaiser, Kiran I. Masood, Zara Ghous, Areeba Hussain, Natasha Bahadur Ali, Syed Hani Abidi, Kulsoom Ghias, Erum Khan, Zahra Hasan

Department of Pathology and Laboratory Medicine

Introduction: An estimated 1.5 million cases were reported in Pakistan until 23 March, 2022. However, SARS-CoV-2 PCR testing capacity has been limited and the incidence of COVID-19 infections is unknown. Volunteer healthy blood donors can be a control population for assessment of SARS-CoV-2 exposure in the population. We determined COVID-19 seroprevalence during the second pandemic wave in Karachi in donors without known infections or symptoms in 4 weeks prior to enrollment.
Materials and methods: We enrolled 558 healthy blood donors at the Aga Khan University Hospital between December 2020 and February 2021. ABO blood groups were determined. Serum IgG reactivity …

Role Of Nucleobindin-2/Nesfatin-1 In The Regulation Of Energetic Status And Maternal Mouthbrooding In The Cichlid Fish, Astatotilapia Burtoni, Saachi Chugh

LSU Master's Theses

Feeding and energetics are fundamental processes in all species that must also be balanced with reproductive investment. The peptide hormone nesfatin-1 is encoded by the nucleobindin-2(nucb2) gene and has recently emerged as a key player involved in both feeding and reproduction across vertebrates. How nesfatin-1 might help regulate trade-offs between feeding and reproduction, however, are relatively unexplored. The female cichlid fish Astatotilapia burtoniis an ideal system to examine the role of nesfatin-1 in regulating metabolic and reproductive functions because this species cycles between a gravid feeding state while preparing to mate, followed by a forced starvation …

Lenses, Focus, And Fluidity: Lessons From Medieval Queer History, Reese Hollister

The Gettysburg Historical Journal

The Medieval era is sometimes overlooked within the field of Queer and Transgender History, but a recent shift in focus has revealed new discoveries and interpretations. This historiographical analysis posits that in the Middle Ages, gender and sexuality were much more fluid than previously believed.

Spatial Analysis Of Health Services In The District Of Tubas, West Bank, Palestine, Muhammad Abu-Salah, Mahmoud Abu-Shama, Hussein Al-Rimmawi

Al-Balqa Journal for Research and Studies البلقاء للبحوث والدراسات

District of Tubas in Palestine has been under occupation for over a half century. The occupying power did not take the issue of developing health services in this district seriously. Therefore, this study, through using new technologies tries to carry out spatial analysis of health services in terms of their distribution and adequacy. This study aims to carry out spatial analysis to health services in Tubas District in terms of their distribution and adequacy. The study also aims to find out the most important problems they encounter and suggest appropriate solutions. Researchers used historical method to follow Tubas District historical …

The Effect Of A Physical Training Program On Several Body Variables And Depression Degree Of Female Trainees While Banned During The Corona Pandemic, Samira M. Orabi, Manal M. Bayyat, Naema Abdusllam Oun

Al-Balqa Journal for Research and Studies البلقاء للبحوث والدراسات

Due to the global COVID 19 pandemic, a curfew was imposed to eliminate the spread of the virus. This study aims to investigate the effect of a training program on several body variables and depression degree of female trainees while banned during the Corona pandemic. The study sample consisted of 20 female trainees attending the Consultative Center in the city of Al- Zawia west / Libya. The proposed training program included rhythmic aerobic exercises accompanied with music for 8 weeks, 3 sessions/week for 45 minutes per session. Researchers have used different tools to measure body variables and the “The Beck …

Blood-Flow-Restriction-Training-Induced Hormonal Response Is Not Associated With Gains In Muscle Size And Strength, Gilberto C. Laurentino, Jeremy P. Loenneke, Carlos Ugrinowitsch, Marcelo S. Aoki, Antonio G. Soares, Hamilton Roschel, Valmor Tricoli

Faculty and Student Publications

The aim of this study was to determine whether increases in post-exercise endocrine response to low-load resistance exercise with blood flow restriction and high-load resistance exercise would have association with increases in muscle size and strength after an 8-week training period. Twenty-nine untrained men were randomly allocated into three groups: low-load resistance exercise with (LL-BFR) or without blood flow restriction (LL), and high-load resistance exercise (HL). Participants from LL-BFR and LL groups performed leg extension exercise at 20% of one repetition maximum (1RM), four sets of 15 repetitions and the HL group performed four sets of eight repetitions at 80% …

Explicit And Implicit Emotion Processing In The Cerebellum: A Meta‑Analysis And Systematic Review, Jordan E. Pierce, Marine Thomasson, Philippe Voruz, Garance Selosse, Julie Péron

Center for Brain, Biology, and Behavior: Faculty Publications

The cerebellum’s role in affective processing is increasingly recognized in the literature, but remains poorly understood, despite abundant clinical evidence for affective disruptions following cerebellar damage. To improve the characterization of emotion processing and investigate how attention allocation impacts this processing, we conducted a meta-analysis on task activation foci using GingerALE software. Eighty human neuroimaging studies of emotion including 2761 participants identified through Web of Science and ProQuest databases were analyzed collectively and then divided into two categories based on the focus of attention during the task: explicit or implicit emotion processing. The results examining the explicit emotion tasks identified …

Ms 059 Guide To Samuel Dreizen, Dds, Md Papers (1946-1992), Samuel Dreizen (1918-1994)

Manuscript Finding Aids

The Samuel Dreizen, DDS, MD papers contains 35mm slides, glass slides, reprints, medical and dental school course notes, lecture notes, faculty workshop records, and models that document Dr. Dreizen's education, work, and colleagues in the field of dentistry. Collection includes records from University of Texas Health Science Center at Houston and UT Dental Branch. See more at MS 059.

Hawks On The Hunt, Sarah Schaier

UNH Today Archive

No abstract provided.

Impacts Of Ocean-Atmosphere Teleconnection Patterns On The South-Central United States, Robert V. Rohli, Gregg A. Snedden, Elinor R. Martin, Kristine L. Delong

Faculty Publications

Recent research has linked the climate variability associated with ocean-atmosphere teleconnections to impacts rippling throughout environmental, economic, and social systems. This research reviews recent literature through 2021 in which we identify linkages among the major modes of climate variability, in the form of ocean-atmosphere teleconnections, and the impacts to temperature and precipitation of the South-Central United States (SCUSA), consisting of Arkansas, Louisiana, New Mexico, Oklahoma, and Texas. The SCUSA is an important areal focus for this analysis because it straddles the ecotone between humid and arid climates in the United States and has a growing population, diverse ecosystems, robust agricultural …

Clinical Characteristics, Prognostic Factors And Treatment Outcomes Of Patients With Bone-Only Metastatic Breast Cancer, Lina Marie, Dina Braik, Nayef H. Abdel-Razeq, Hala Abu-Fares, Ahmad Al-Thunaibat, Hikmat Abdel-Razeq

Internal Medicine Articles

Introduction: Bone is the most frequent site of breast cancer metastasis. Differences between those who present with de novo bone-only metastasis (BOM) and those who progress to bone-only disease following a diagnosis of early-stage breast cancer are not clear. Such differences in clinical course might have an impact on the aggressiveness of treatment. This study presents the clinical and pathological features, along with treatment outcomes, of breast cancer patients with BOM in relation to the timing and type of bone metastasis.

Patients and Methods: Patients with breast cancer and BOM were retrospectively reviewed. De novo BOM was defined as bone …

Niche Partitioning Of Food Resources By Freshwater Mussels In A Multispecies Mussel Bed In The Sabine River, Marissa Netti

Biology Theses

Freshwater mussels (Unionids) are a very diverse group of bivalves, with about 300 species in the United States. Although freshwater mussels are very diverse, they are also critically endangered and are declining at extremely high rates. In fact, the U.S. Fish and Wildlife Service proposed excluding eight freshwater mussel species from the Endangered Species Act (ESA) in 2021 because they went extinct. Mussels play a key role in keeping our water systems healthy because of their filter feeding method. Filter feeding influences the rest of the ecosystem through the transfer of energy, cycling of nutrients, and purification of the surrounding …

Drift Macroalgal Distribution In Northern Gulf Of Mexico Seagrass Meadows, Kelly M. Correia, Scott B. Alford, Benjamin A. Belgrad, Kelly M. Darnell, M. Zachary Darnell, Bradley T. Furman, Margaret O. Hall, Christian T. Hayes, Charles W. Martin, Ashley M. Mcdonald, Delbert L. Smee

Faculty Publications

Drift macroalgae, often found in clumps or mats adjacent to or within seagrass beds, can increase the value of seagrass beds as habitat for nekton via added food resources and structural complexity. But, as algal biomass increases, it can also decrease light availability, inhibit faunal movements, smother benthic communities, and contribute to hypoxia, all of which can reduce nekton abundance. We quantified the abundance and distribution of drift macroalgae within seagrass meadows dominated by turtle grass Thalassia testudinum across the northern Gulf of Mexico and compared seagrass characteristics to macroalgal biomass and distribution. Drift macroalgae were most abundant in areas …

Future Crop Risk Estimation Due To Drought, Extreme Temperature, Hail, Lightning, And Tornado At The Census Tract Level In Louisiana, Rubayet Bin Mostafiz, Robert V. Rohli, Carol J. Friedland, Melanie Gall, Nazla Bushra

Faculty Publications

Louisiana is one of the most hazard-prone states in the U.S., and many of its people are engaged directly or indirectly in agricultural activities that are impacted by an array of weather hazards. However, most hazard impact research on agriculture to date, for Louisiana and elsewhere, has focused on floods and hurricanes. This research develops a method of future crop loss risk assessment due to droughts, extreme low and high temperatures, hail, lightning, and tornadoes, using Louisiana as a case study. This approach improves future crop risk assessment by incorporating historical crop loss, historical and modeled future hazard intensity, cropland …

Metabolic Model Of Necrotizing Enterocolitis In The Premature Newborn Gut Resulting From Enteric Dysbiosis, Giorgio Casaburi, Jingjing Wei, Sufyan Kazi, Junlin Liu, Kewei Wang, Guo-Zhong Tao, Po-Yu Lin, James C. Y. Dunn, Bethany M. Henrick, Steven A. Frese, Karl G. Sylvester

Department of Nutrition and Health Sciences: Faculty Publications

Necrotizing enterocolitis (NEC) is a leading cause of premature newborn morbidity and mortality. The clinical features of NEC consistently include prematurity, gut dysbiosis and enteral inflammation, yet the pathogenesis remains obscure. Herein we combine metagenomics and targeted metabolomics, with functional in vivo and in vitro assessment, to define a novel molecular mechanism of NEC. One thousand six hundred and forty seven publicly available metagenomics datasets were analyzed (NEC = 245; healthy = 1,402) using artificial intelligence methodologies. Targeted metabolomic profiling was used to quantify the concentration of specified fecal metabolites at NEC onset (n = 8), during recovery ( …

Image Analysis For Characterization Of Microtubule Protrusions, Haomin Yu

Mechanical Engineering and Materials Science Independent Study

The dynamics of microtubules is central to how cells probe their environment and adopt both morphology and phenotypic fate. The length and dynamics of the protrusions that microtubules drive may be affected by the growth environment of a cell. This independent study project aimed to obtain data on the relationship between environment and microtubule disposition through integrated experiments and analysis. PDMS models in different sizes were fabricated by photolithography technology. The diameters of the PDMS model used in this experiment were 50, 75, 100, and 200 microns, respectively. A single 3T3 cell was grown in the PDMS model. After the …

Energy Drink Usage By Students Attending An Online University, Jodi Lynn Hutak, Ali Boolani, Lauri O. Byerley

School of Medicine Faculty Publications

Objective: This study determined the frequency, reasons for, and side effects of energy drinks (ED) consumption among online students. Participants: Students attending an online university. Methods: Participants were recruited by email and completed a 59-question survey about their prior months ED practices using a combination of validated surveys previously published examining similar constructs in on-campus students. Results: 307 students (age = 32.4 ± 6.5yrs) completed the survey, and 88% reported consuming EDs. Students’ reasons for consuming ED included school (p < .001), work (p < .001), an event/competition (p < .001), pick me up (p < .001), lack of rest (p < .001), more energy (p < .001), and staying awake while driving (p < .001). Only individuals who consumed > 10 ED/month reported side effects of headaches (p = .01) and speeding (p=.01). Conclusions: In our sample, a majority of …

A Yield Comparison Between Small-Plot And On-Farm Foliar Fungicide Trials In Soybean And Maize, Anabelle Laurent, Emily Heaton, Peter Kyveryga, David Makowski, Laila A. Puntel, Alison E. Robertson, Laura J. Thompson, Fernando Miguez

Department of Agronomy and Horticulture: Faculty Publications

Agronomic research provides management recommendations based on small-plot trials (SPTs) and on-farm trials (OFTs) with very different characteristics. SPTs are traditionally conducted at agricultural experiment stations by research institutes or universities, while OFTs are conducted under commercial-scale conditions and managed by farmers using their own equipment. Several researchers claimed that discrepancies could occur between these two types of trials, which can make the extrapolation of results from SPTs to the farm level difficult. In our study, we conducted an extensive comparison of small-plot and on-farm trials to analyze the effect of foliar fungicide application on maize and soybean yields. We …

Great-Tailed Grackles (Quiscalus Mexicanus) As A Tolerant Host Of Avian Malaria Parasites, M. Andreína Pacheco, Francisco C. Ferreira, Corina J. Logan, Kelsey B. Mccune, Maggie P. Macpherson, Sergio Albino Miranda, Diego Santiago-Alarcon, Ananias A. Escalante

Harold W. Manter Laboratory of Parasitology: Library Materials

Great-tailed Grackles (Quiscalus mexicanus) are a social, polygamous bird species whose populations have rapidly expanded their geographic range across North America over the past century. Before 1865, Great-tailed Grackles were only documented in Central America, Mexico, and southern Texas in the USA. Given the rapid northern expansion of this species, it is relevant to study its role in the dynamics of avian blood parasites. Here, 87 Great-tailed grackles in Arizona (a population in the new center of the range) were screened for haemosporidian parasites using microscopy and PCR targeting the parasite mitochondrial cytochrome b gene. Individuals were caught …

Advancing Student Futures In Stem, Urmi Ghosh-Dastidar, Sandie Han, Nadia Kennedy, Diana Samaroo Phd, Armando Solis

Publications and Research

This work reports a programmatic effort devoted to increasing diversity, equity, and inclusion in STEM education at a Hispanic-serving undergraduate higher-education institution. Between Fall 2015 and Spring 2020, the STEM program offered comprehensive academic and financial support to ninety-four students from five STEM majors: Bachelor of Science degrees in Biomedical Informatics, Applied Chemistry, and Applied Mathematics; and Associate of Science degrees in Chemical Technology and Computer Science. The goals of the program were to: (1) support, retain and graduate academically talented low-income and underrepresented minority students in the five targeted STEM majors; (2) establish a model for a comprehensive support …

2021 Btl O&M Events Log / 2021 Daily Log, Pioneer Technical Services, Inc., Environmental Protection Agency

Silver Bow Creek/Butte Area Superfund Site

No abstract provided.

Morphodynamics Of Wave-Dominated Beaches, B Castelle, G Masselink

School of Biological and Marine Sciences

Abstract Wave-dominated sandy beaches are highly valued by societies and are amongst the world’s most energetic and dynamic environments. On wave-dominated beaches with unlimited sand supply and limited influence of tide and geology, beach change has long been conceptualised in the morphodynamic framework of Wright and Short (1984). Such framework describes the occurrence of beach types based on wave conditions and sediment characteristics across the complete reflective–dissipative spectrum. Building on theoretical work, field/laboratory measurements and monitoring programmes, the physical mechanisms underpinning this morphodynamic framework have been progressively unravelled. Cross-shore morphological changes are primarily controlled by equilibrium and beach memory principles …

Update On The Management Of Vitamins And Minerals In Cystic Fibrosis, Senthilkumar Sankararaman

Faculty Scholarship

Advancements in respiratory and nutrition management have significantly improved the survival of patients with cystic fibrosis (CF). With the availability of several nutrition interventions such as oral/enteral nutrition supplements, enteric-coated pancreatic enzymes, and water-miscible CF-specific vitamin supplements, frank vitamin deficiencies—with the exception of vitamin D—are rarely encountered in current clinical practice. Whereas they were previously considered as micronutrients, our current understanding of fat-soluble vitamins and minerals as antioxidants, immunomodulators, and disease biomarkers has been evolving. The impact of highly effective modulators on the micronutrient status of patients with CF remains elusive. This narrative review focuses on the updates on the …

Two Interaction Surfaces Between Xpa And Rpa Organize The Preincision Complex In Nucleotide Excision Repair, Mihyun Kim, Hyun-Suk Kim, Areetha D'Souza, Kaitlyn Gallagher, Eunwoo Jeong, Agnieszka Topolska-Wós, Kateryna Ogorodnik Le Meur, Chi-Lin Tsai, Miaw-Sheue Tsai, Minyong Kee, John A Tainer, Jung-Eun Yeo, Walter J Chazin, Orlando D Schärer

Faculty, Staff and Student Publications

The xeroderma pigmentosum protein A (XPA) and replication protein A (RPA) proteins fulfill essential roles in the assembly of the preincision complex in the nucleotide excision repair (NER) pathway. We have previously characterized the two interaction sites, one between the XPA N-terminal (XPA-N) disordered domain and the RPA32 C-terminal domain (RPA32C), and the other with the XPA DNA binding domain (DBD) and the RPA70AB DBDs. Here, we show that XPA mutations that inhibit the physical interaction in either site reduce NER activity in biochemical and cellular systems. Combining mutations in the two sites leads to an additive inhibition of NER, …

C10pred: A First Machine Learning Based Tool To Predict C10 Family Cysteine Peptidases Using Sequence-Derived Features, Adeel Malik, Nitin Mahajan, Tanveer Ali Dar, Chang-Bae Kim

2020-Current year OA Pubs

No abstract provided.

Whole Genome Association Study Of The Plasma Metabolome Identifies Metabolites Linked To Cardiometabolic Disease In Black Individuals, Usman A. Tahir, Daniel H. Katz, Julian Avila-Pachecho, Alexander G. Bick, Akhil Pampana, John Blangero, Joanne Curran, Juan M. Peralta, Harald H. H. Goring, Michael Mahaney

School of Medicine Publications

Integrating genetic information with metabolomics has provided new insights into genes affecting human metabolism. However, gene-metabolite integration has been primarily studied in individuals of European Ancestry, limiting the opportunity to leverage genomic diversity for discovery. In addition, these analyses have principally involved known metabolites, with the majority of the profiled peaks left unannotated. Here, we perform a whole genome association study of 2,291 metabolite peaks (known and unknown features) in 2,466 Black individuals from the Jackson Heart Study. We identify 519 locus-metabolite associations for 427 metabolite peaks and validate our findings in two multi-ethnic cohorts. A significant proportion of these …

Climate Warming Amplifies The Frequency Of Fish Mass Mortality Events Across North Temperate Lakes, Simon P. Tye, Adam M. Siepielski, Andrew Bray, Andrew L. Rypel, Nicholas B.D. Phelps, Samuel B. Fey

Biological Sciences Faculty Publications and Presentations

Recent increases of animal mass mortality events have coincided with substantial changes in global climate. Yet, tractable approaches that predict how climate change will accentuate occurrences of these ecological catastrophes remain nascent. We compiled one of the most comprehensive datasets of lentic fish mortality events, thermal tolerances of affected families, and 1.2 million air and water temperature profiles across 8891 north temperate lakes in North America. Temperature extremes within and across lakes were strongly associated with the three most frequent cause types (infectious agents, summerkills, winterkills). Thermal tolerances mediated the lethality of direct thermal stress, but mortalities of warm- and …

Estimating Uncertainty In Density Surface Models, David L. Miller, Elizabeth A. Becker, Karin A. Forney, Jason J. Roberts, Ana Cañadas, Robert S. Schick

Faculty Research, Scholarly, and Creative Activity

Providing uncertainty estimates for predictions derived from species distribution models is essential for management but there is little guidance on potential sources of uncertainty in predictions and how best to combine these. Here we show where uncertainty can arise in density surface models (a multi-stage spatial modelling approach for distance sampling data), focussing on cetacean density modelling. We propose an extensible, modular, hybrid analytical-simulation approach to encapsulate these sources. We provide example analyses of fin whales Balaenoptera physalus in the California Current Ecosystem.