Digital Commons Network^™

Meaningless Dna: Moore’S Inadequate Protection Of Genetic Material, Natalie Alexander

Dickinson Law Review (2017-Present)

Moore v. Regents of the University of California represents the seminal case regarding the protection of genetic material. In this case, the California Supreme Court held that patients do not retain property rights in their excised genetic material; instead, informed consent laws serve as genetic material’s only protection. Many states have accepted the Moore court’s decision not to extend property rights to genetic material, and most states choose to protect genetic material through informed consent alone. Moore and informed consent do not adequately protect genetic material, creating unjust results in which “donors” of genetic material have little to no recourse …

Ccl4 Regulates Eosinophil Activation In Eosiccl4 Regulates Eosinophil Activation In Eosinophilic Airway Inflammationnophilic Airway Inflammation, Hanh Hong Chu, Yoshiki Kobayashi, Dan Van Bui, Yasutaka Yun, Linh Manh Nguyen, Akitoshi Mitani, Kensuke Suzuki, Mikiya Asako, Akira Kanda, Hiroshi Iwai

Faculty, Staff and Student Publications

Eosinophilic chronic rhinosinusitis (ECRS) is a refractory airway disease accompanied by eosinophilic inflammation, the mechanisms of which are unknown. We recently found that CCL4/MIP-1β-a specific ligand for CCR5 receptors-was implicated in eosinophil recruitment into the inflammatory site and was substantially released from activated eosinophils. Moreover, it was found in nasal polyps from patients with ECRS, primarily in epithelial cells. In the present study, the role of epithelial cell-derived CCL4 in eosinophil activation was investigated. First, CCL4 expression in nasal polyps from patients with ECRS as well as its role of CCL4 in eosinophilic airway inflammation were investigated in an in …

Nuclear S6k1 Enhances Oncogenic Wnt Signaling By Inducing Wnt/Β-Catenin Transcriptional Complex Formation, Min Gyu Lee, Hwamok Oh, Jong Woo Park, Jueng Soo You, Jeung-Whan Han

Faculty, Staff and Student Publications

Ribosomal protein S6 kinase 1 (S6K1), a key downstream effector of the mammalian target of rapamycin (mTOR), regulates diverse functions, such as cell proliferation, cell growth, and protein synthesis. Because S6K1 was previously known to be localized in the cytoplasm, its function has been mainly studied in the cytoplasm. However, the nuclear localization and function of S6K1 have recently been elucidated and other nuclear functions are expected to exist but remain elusive. Here, we show a novel nuclear role of S6K1 in regulating the expression of the Wnt target genes. Upon activation of the Wnt signaling, S6K1 translocated from the …

Ex Vivo Drug Sensitivity Correlates With Clinical Response And Supports Personalized Therapy In Pediatric Aml, Debbie C Strachan, Christine J Gu, Ryosuke Kita, Erica K Anderson, Michelle A Richardson, George Yam, Graham Pimm, Jordan Roselli, Alyssa Schweickert, Maci Terrell, Raushan Rashid, Alan K Gonzalez, Hailey H Oviedo, Michelle C Alozie, Tamilini Ilangovan, Andrea N Marcogliese, Hiroomi Tada, Marianne T Santaguida, Alexandra M Stevens

Faculty, Staff and Students Publications

Acute myeloid leukemia (AML) is a heterogeneous disease that accounts for ~20% of all childhood leukemias, and more than 40% of children with AML relapse within three years of diagnosis. Although recent efforts have focused on developing a precise medicine-based approach towards treating AML in adults, there remains a critical gap in therapies designed specifically for children. Here, we present ex vivo drug sensitivity profiles for children with de novo AML using an automated flow cytometry platform. Fresh diagnostic blood or bone marrow aspirate samples were screened for sensitivity in response to 78 dose conditions by measuring the reduction in …

"I Am Primarily A Scientific Philologist": J.R.R. Tolkien And The Science/Technology Divide, Kristine Larsen

Journal of Tolkien Research

Presentation on Tolkien's writings on science and technology, New York Tolkien Conference, Baruch College, New York, March 17, 2019

Commencement [Program], December 17, 2022, University Of Northern Iowa

UNI Commencement Programs

The program of the commencement ceremonies, including a list of institutional leadership, a list of the departments by college, awards given at the ceremonies, and a list of undergraduate and graduate degree candidates. This commencement program is not an official graduate list.

Roles Of Individual Protein In De Novo Polycomb Protein Recruitment, Piao Ye

Biological Sciences Theses and Dissertations

Polycomb Group (PcG) proteins are evolutionarily conserved epigenetic transcriptional regulators that maintain the transcriptional repression of silenced genes by maintaining heritable chromatin states. PcG proteins first discovered as repressors of Hox genes in Drosophila, later were shown to regulate a wide range of genes. In mammals, PcG proteins are involved in maintaining pluripotent state of stem cells and controlling cell differentiation. Misexpression of PcG protein leads to cancers like lymphoma and melanoma. PcG proteins maintain rather than initiate transcriptional repression, once PcG – mediated repression is established it can be maintained through an unlimited number of cycles. Most studies of …

Dissecting Interactions Across Gene Regulatory Layers In C. Elegans, Morgan Taylor

Biological Sciences Theses and Dissertations

The nematode Caenorhabditis elegans is a powerful tool for studying nervous system genetics. Though relatively simple compared to mammals, C. elegans boasts a remarkably well-conserved neuronal genome and proteome, and its utility in the characterization of neuronal genes has been well-established. However, gene expression is often controlled by complex interactions between multiple genes, and teasing apart the functions of individual genes within such networks remains a challenge. Dissecting these interaction networks is crucial in determining the multifaceted functions of important, conserved regulatory genes. Here we explore interactions between gene regulatory layers in the C. elegans nervous system, employing a synthetic …

Cryptic Population Structure And Differentiation In Three Species Of Alaska Claytonia, Robert W. Gabbitas

Biological Sciences

Beringia is an extensive geographic region noted for high levels of taxonomic complexity among closely related species. Three such species distributed across Beringia (Claytonia arctica, C. scammaniana, and C. sarmentosa,) exhibit low morphological differentiation between the species, while C. scammaniana includes two additional described morphologies of questionable taxonomic standing, C. "noatakensis" and C. "porsildii". Taxonomic boundaries within this group have been historically disagreed upon and are susceptible to confounding factors such as isolation-by-distance (IBD), discontinuous sampling of continuous variation, and low sequence divergence and morphological differentiation. To assist in species delimitation, multiple approaches combining phylogenomics and spatially explicit population genetics …

Thermokarst-Pond Plant Community Characteristics And Effects On Icewedge Degradation In The Prudhoe Bay Region, Alaska, Emily Watson-Cook

Biological Sciences

Ice-wedge thermokarst ponds are forming in many areas of the Arctic as a result of climate warming and infrastructure development. Previous research suggests that development of aquatic vegetation within these ponds may create negative feedbacks to the process of ice-wedge degradation by reducing pond-bottom temperatures and thaw depths. The objectives of this research were to characterize thermokarst-pond plant communities and to evaluate the effects of vegetation on within-pond sediment temperatures and thaw depths. Aquatic vegetation was sampled in 39 plots within 29 thermokarst ponds in the Prudhoe Bay region of Alaska. Five floristically distinct plant communities were identified: Calliergon richardsonii …

Shifts In Microbial Community Composition During The 2019 Pacific Marine Heatwave In The Northern Gulf Of Alaska, Jacob Cohen

Oceanography

Climate change has led to a gradual increase of sea surface temperatures in the Northern Gulf of Alaska (NGA) interspersed with marine heatwaves (MHW) that impose a rapid but temporary perturbation of sea surface temperature. MHWs have the potential to alter marine microbial community structure, which may impact the production and transfer of carbon to higher trophic levels. The year 2019 was characterized as an MHW in the North Pacific, with sea surface temperatures in the NGA reaching ~2.5 º C above average during 2019 and ~1 º C above average during 2020, while 2021 had near-average sea surface temperatures. …

Development Of A Muc1 Cancer Immunotherapy Using Complement Targeted Liposomes And Organoplatinum (Iv) Complex As An Effective Anticancer Drug, Ameneh Arabi

Chemistry and Biochemistry

The development of therapeutic and preventive cancer vaccines has been on the rise in the past decade. Extensive tumor antigen libraries supply researchers with a wide variety of targets for developing cancer vaccines. Cancer vaccines aim to induce tumor regression, eradicate tumors and establish lasting antitumor memory, while avoiding adverse reactions. However, achieving this goal is hampered by the challenges of finding tumor antigens to incite an effective immune response and creating an efficient delivery system to deliver the antigen to target cells. In this thesis, we discuss how to integrate Toll Like Receptor (TLR) agonists with a MUC1 cancer …

Exploring The Evolution Of Fishes At High Latitudes, Anna S. Rix

Biological Sciences

Fish species found in high latitude waters are especially vulnerable to climatic changes due to their inability to regulate body temperature and long evolution in cold, oxygen-rich aquatic environments. Antarctic notothenioid fishes have evolved to thrive in an extremely stable, cold, oxygen-rich environment. Likewise, lake trout live in a cold, oxygen-rich environment, but commonly experience a wider range of temperatures than notothenioids. Millions of years of evolution in the cold have shaped the genetics of these fishes, but the effects on their biology remain largely unexplored. This dissertation seeks to learn from the past evolution of these two evolutionarily distant …

Potential Benefits Of The Campus Outdoor Built Environment On Student Stress, Leading To Improved Retention, Kevin Dilliard

Graduate Liberal Studies Theses and Dissertations

University landscape managers often ask themselves, are they making a difference? Since what they do is not a revenue-generating department, how can they validate their true worth beyond making the campus beautiful, which is excellent, but what added value do they bring? As is common practice, when economic times get tough, the landscape department budget is one of the first to be cut, but is that the wisest thing to do? This research was intended to explore the concept that the services landscape managers provide have a return on investment by providing an environment in which students can thrive and …

2022 Fall Commencement, Harding University

Commencement Programs

Program for the Harding University Commencement on December 17.

Commencement Speaker: Susan Hutchinson

Architects Of Abundance: Indigenous Regenerative Food And Land Management Systems And The Excavation Of Hidden History, Lyla June Johnston

Indigenous Studies

Pre-Columbian and contemporary Indigenous Nations of North and South America (hereafter referred to as Turtle Island and Abya Yala) have managed ecosystems extensively to produce prolific and predictable food systems for themselves and non-humans, whom they often view as relatives. The elements of earth, water, fire, and air are explored to analyze Indigenous soil management, Indigenous aquaculture, Indigenous pyrogenic land management, and Indigenous oral histories, respectively. First, a review of four Indigenous societies and their soil management techniques revealed that none of these systems require outside fertilizer or irrigation to sustain ecocentric food systems on millennial scales. Second, a comparative …

I'M A Killer Whale: The Process Of Cultural Identity Development From The Perspectives Of Young Indigenous Children, Angela Lunda

Indigenous Studies

This qualitative single case study examined the phenomenon of cultural identity development from the perspective of young Indigenous children situated within the context of their southeast Alaskan community. Decades of assimilationist policies have eroded cultural identity among many Indigenous Alaskans, yet a strong cultural identity is known to be a protective factor for Indigenous peoples. Building on Indigenous identity development theory, the study sought to answer the research questions: (1) How do young children demonstrate their cultural identity through interactions on the Land? (2) How do community organizations support cultural identity development (CID) in young Indigenous children? (3) What role …

Computing Prime Factorizations With Neural Networks, Dakota Dragomir

Mathematics and Statistics

When dealing with sufficiently large integers, even the most cutting-edge existing algorithms for computing prime factorizations are impractically slow. In this paper, we explore the possibility of using neural networks to approximate prime factorizations in the hopes of providing an alternative factorization method which trades accuracy for speed. Due to the intrinsic difficulty associated with this task, the focus of this paper is largely concentrated on the obstacles encountered in the training of the neural net, rather than on the viability of the method itself.

Implications Of Spatial Population Dynamics For Abundance Estimation And Catch Apportionment Of Alaska Sablefish (Anoplopoma Fimbria), Kari Hammarsten Fenske

Fisheries

Spatial dynamics in fish populations are challenging to identify and incorporate into population dynamics models due to the large amount of data required and the uncertainty associated with spatial processes. However, ignoring spatial dynamics can result in biased population estimates and negatively affect population sustainability or the ability to meet fishery management goals. My research explored implications of spatial dynamics for the assessment and management of Alaska sablefish (Anoplopoma fimbria). Sablefish are highly mobile, targeted in commercial and recreational fisheries, and documented to have spatial variation in population dynamics such as abundance and biomass. However, gaps remain in understanding the …

Freshwater Pressures On Pacific Salmon In The Coastal Watersheds Of Alaska, Christopher J. Sergeant

Fisheries

This dissertation explores the implications of a warmer and wetter climate on the freshwater life stages of Pacific salmon (Oncorhynchus spp.) in the focal region of southern coastal Alaska. Recent trends and long-term climate change predictions support the notion that arctic and subarctic watersheds will be subject to warming air temperatures, increased rainfall in the autumn and winter, diminishing snowpack, and continued glacial recession. Such atmospheric and terrestrial changes will shift patterns of streamflow, water temperature, and habitat diversity that comprise the basic building blocks of freshwater ecosystems that support Pacific salmon. Alaska's Pacific salmon populations sustain a multi-billion-dollar economy …

Sled Dogs As Gifts. An Ethnography Of Dog Exchanges Among Kennels In Alaska., Allison K. Cruz

Anthropology

This thesis investigates the social relationships that develop through the circulation of sled dogs among Alaskan kennels. It is based on multi-sited ethnographic fieldwork at racing events, in sled dog kennels, as well as semi-structured interviews around Fairbanks. The research is informed by anthropological literature on kinship, personhood, and gift-giving economies. It examines how sled dogs are acquired and exchanged in Alaska and offers a novel perspective on the value of dog bloodlines and the relationships that unite breeders, mushers and sled dogs. The main argument is that Alaskan sled dogs are exchanged as gifts (rather than commodities) with the …

Species Distribution Modeling Of Northern Sea Otters (Enhydra Lutris Kenyoni) In A Data-Limited Ecosystem, Elizabeth L. Hasan

Marine Biology

Species distribution models are used to map and predict geographic distributions of animals based on environmental covariates. However, species distribution models often require high resolution habitat data and time-series data on animal locations. In data-limited regions with little animal survey data or habitat information, modeling is more challenging and often ignores important environmental attributes. For sea otters (Enhydra lutris), a federally protected keystone species with variable population trends across their range, predictive modeling of distributions has been successfully conducted in areas with an abundance of sea otter and habitat data. Here, we used open-access data across a single time step …

Acute Toxicity Of Copper To Three Species Of Pacific Salmon In Water With Low Hardness And Low Dissolved Organic Carbon, Drew E. Porter

Marine Biology

Despite a history of investigation, the toxicity of copper (Cu) to fishes remains difficult to predict due to the complex influential effects of water chemistry. Water hardness and dissolved organic carbon (DOC) concentrations can vary significantly within a watershed and also attenuate the toxicity of Cu to fishes. To account for location-specific water chemistry and predict Cu toxicity to aquatic organisms the United States Environmental Protection Agency (USEPA) endorses use of the biotic ligand model (BLM). Though the BLM has proven useful in many instances, it has performed inaccurately for waters low in hardness and DOC; this has raised questions …

Compound-Specific Stable Isotopes Of Amino Acids Reveal The Influence Of Trophic Level And Primary Production Sources On Mercury Concentrations In Fishes From The Aleutian Islands, Alaska, N/A N/A

Marine Biology

Total mercury (THg) concentrations exceed thresholds of concern in some Steller sea lion (Eumetopias jubatus; SSL) tissues from certain portions of the Aleutian Islands, Alaska. Here, compound-specific stable isotope analyses (CSIA) of carbon in essential amino acids ([delta]¹³CEAA values) and nitrogen in AAs ([delta]¹⁵NAA values) in fish muscle tissue was applied to quantify the proportional contributions of primary production sources and trophic positions of eight prey species (n = 474 total) that are part of SSL diets. Previous THg analyses of fish muscle, coupled with additional monomethylmercury (MMHg) analyses of a subset of samples, substantiated previous findings that fishes from …

Drivers Of Functional Ecology Of The Alaskan Arctic Epibenthos, Lauren Sutton

Marine Biology

This dissertation explored multiple facets of functional diversity for epibenthic invertebrate communities of Alaskan Arctic shelves. Functional diversity is the range of organismal traits within a community that determines ecosystem functioning. As a complement to taxonomic diversity, functional diversity reflects what species "do" as opposed to "who" they are, providing information on community-level ecosystem resilience and vulnerability. The Alaskan Arctic marine system is presently changing at an unprecedented rate, which impacts the biomass-rich benthos that is of great importance to upper trophic level fishes, birds, and marine mammals as a food source. In my first chapter, I tested the Biodiversity-Ecosystem-Functioning …

Exploring The North American Arctic Benthos: Community Structure And Oil Degradation Potential Of Sediment Bacteria And Archaea, Alexis M. Walker

Marine Biology

The Chukchi and Beaufort seas benthic habitats are home to a multitude of ecologically and commercially important organisms that are subject to ongoing environmental changes, including the impacts of climate change and increased exposure to contaminants. Benthic bacteria and archaea can be considered biogeochemical engineers. They play a major role in organic matter (OM) degradation and nutrient cycling and their community structure can reflect changes in environmental conditions such as OM composition and quantity, nutrient availability, redox conditions, and natural/anthropogenic contaminants (e.g. petroleum hydrocarbons). Yet, sediment microbial communities have rarely been examined in these marginal seas of North American Arctic. …

Seaweeds Across Ecosystem Boundaries: From Habitat Formation To Harvest Implications, Brian P. Ulaski

Marine Biology

This dissertation broadly investigates the response of wild stock seaweeds to harvesting, and their role as biogenic habitat formers when cast ashore. Seaweeds are important primary producers and foundation species that maintain their functional roles across coastal ecosystem boundaries. Climate-driven loss of seaweed biomass, including that of kelp forests, is exacerbated by other human-related activities that directly impact their persistence, such as overharvesting. Attached rockweeds and kelps are commonly harvested for food, while diverse assemblages of beach-cast seaweed wrack are collected for fertilizer. The importance of wrack habitat in Alaska has not been extensively explored, especially in regions where there …

Inhibition Of Rad18 By Arsenic, Lindsay B. Volk

Biomedical Sciences ETDs

Arsenite exposure leads to the retention of UV-induced DNA damage, thus burdening translesion synthesis (TLS). Rad18 is an essential factor in initiating TLS through PCNA monoubiquitination and is implicated in homologous recombination. It contains two functionally and structurally distinct zinc fingers that are potential targets for arsenite binding. Results from this study reveal arsenite binding to both zinc fingers of Rad18 and a corresponding loss of domain function. Importantly, arsenite inhibited Rad18 RING-dependent PCNA monoubiquitination and polymerase eta recruitment to DNA damage. Further analysis demonstrated multiple effects of arsenite, including the reduction in the nuclear localization and UV-induced chromatin recruitment …

Spatial Single-Cell Identification Of Cell Layers Within Mouse Brain Using A Novel Spatially Resolved Transcriptomics Technology, Radha Swaminathan

Biomedical Engineering ETDs

Spatially resolved transcriptomics technologies investigate gene expression in the context of a tissue. While imaging-based technologies enable gene expression quantification at a single-cell level, only two NGS-based technologies are currently capable of single-cell resolution. Both Stereo-seq and Seq-Scope involve two rounds of sequencing: one to sequence the location of spatial barcodes and the other to obtain sequence of cDNA. Thus, the objective of the research study is to develop a technology that can capture transcripts from every cell of a tissue without the need for two rounds of sequencing. To this end, a capture surface or barcoded array (similar to …

In Vitro Anti-Helicobacter Pylori And Antioxidant Activities Of Plants Used In Algerian Traditional Medicine For Gastrointestinal Disorders, Souhila Tabak, Bendif Hamdi, Mohamed Djamel Miara, Luca A. Vitali

Journal of Bioresource Management

It has been recognized that gastric colonization with Helicobacter pylori (H. pylori) induces a risk of developing ulcer disease and gastric cancer. The interest of this research relates for the first time to the study of the Anti-Helicobacter pylori activities of three medicinal plants methanolic extracts, namely Allium sativum, Allium cepa and Foeniculum vulgare. The activity of the methanolic extracts was assessed against two strains of H. pylori (SAN158 and 26659) by the disc diffusion method on Muller Hinton agar. The content of phenolic compounds (TPC) was determined by the Folin-Ciocalteu test and the antioxidant …