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2022

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Biological And Phytochemical Studies On Six Astragalus Taxa From Anatolia, Sophie Fadime Aydoğan, Zulfiqar Ali, Shabana I. Khan, Jianping Zhao, Ikhlas A. Khan Oct 2022

Biological And Phytochemical Studies On Six Astragalus Taxa From Anatolia, Sophie Fadime Aydoğan, Zulfiqar Ali, Shabana I. Khan, Jianping Zhao, Ikhlas A. Khan

Annual Poster Session 2022

Corresponding author (NCNPR): Fadime Aydoğan, [email protected]


Utility Of Fatty Acid Profile And In Vitro Immune Cell Activation For Chemical And Biological Standardization Of Arthrospira/Limnospira, Jungmoo Huh, Jin Zhang, Radka Hauerová, Joseph Lee, Saqlain Haider, Mei Wang, Tomáš Hauer, Ikhlas A. Khan, Amar G. Chittiboyina, Nirmal D. Pugh Oct 2022

Utility Of Fatty Acid Profile And In Vitro Immune Cell Activation For Chemical And Biological Standardization Of Arthrospira/Limnospira, Jungmoo Huh, Jin Zhang, Radka Hauerová, Joseph Lee, Saqlain Haider, Mei Wang, Tomáš Hauer, Ikhlas A. Khan, Amar G. Chittiboyina, Nirmal D. Pugh

Annual Poster Session 2022

Corresponding author (NCNPR): Jin Zhang, [email protected]


Department Of Biomolecular Sciences, Candace Lowstuter Oct 2022

Department Of Biomolecular Sciences, Candace Lowstuter

Annual Poster Session 2022

Department/Unit poster (BioMolecular Sciences). Corresponding author: Candace Lowstuter ([email protected])


Improved Topical Ophthalmic Natamycin Suspension For The Treatment Of Fungal Keratitis, Chuntian Cai, Poorva Joshi, Ahmed Adel Ali Youssef, Narendar Dudhipala, Soumyajit Majumdar Oct 2022

Improved Topical Ophthalmic Natamycin Suspension For The Treatment Of Fungal Keratitis, Chuntian Cai, Poorva Joshi, Ahmed Adel Ali Youssef, Narendar Dudhipala, Soumyajit Majumdar

Annual Poster Session 2022

Corresponding author (Pharmaceutics and Drug Delivery): Chuntian Cai, [email protected]


Effect Of Ploidy On Early Oyster Life Stage Tolerance Of Salinity And Temperature, Christian Boudreaux, Alexz Carpenter, Jessica Pruett, Kristine Willett, Deborah Gochfeld Oct 2022

Effect Of Ploidy On Early Oyster Life Stage Tolerance Of Salinity And Temperature, Christian Boudreaux, Alexz Carpenter, Jessica Pruett, Kristine Willett, Deborah Gochfeld

Annual Poster Session 2022

Corresponding author (BioMolecular Sciences): Christian Boudreaux, [email protected]


Effects Of Chemical Inducers On Larval Oyster Settlement In Suboptimal Conditions, Alexz Carpenter, Christian Boudreaux, Jessica Pruett, Kristine Willett, Deborah Gochfeld Oct 2022

Effects Of Chemical Inducers On Larval Oyster Settlement In Suboptimal Conditions, Alexz Carpenter, Christian Boudreaux, Jessica Pruett, Kristine Willett, Deborah Gochfeld

Annual Poster Session 2022

Corresponding author (Biological Science): Alexz Carpenter, [email protected]


Ms 242 Guide To The John Z. Bowers, Md Papers (1947-1983), John Z. Bowers (1913-1993) Oct 2022

Ms 242 Guide To The John Z. Bowers, Md Papers (1947-1983), John Z. Bowers (1913-1993)

Manuscript Finding Aids

This collection includes photographs, manuscripts, and reprints related to Dr. John Bowers' involvement with the Atomic Bomb Casualty Commission (ABCC). Of particular note are 58 black-and-white photographs of Nagasaki, Japan, taken by Captain Shields Warren (MC) in 1947. There is also a photograph showing Dr. Bowers alongside Carl Tessmer reviewing plans for the ABCC laboratory-clinic in Hiroshima, and another featuring the groundbreaking of the Radiation Effects Research Foundation (RERF). A third set of photographs depicts Berlin, Germany during the Cold War. See more at MS 242.


Rice Chalky Grain 5 Regulates Natural Variation For Grain Quality Under Heat Stress, Anil Kumar Nalini Chandran, Jaspreet Sandhu, Larissa Irvin, Puneet Paul, Balpreet K. Dhatt, Waseem Hussain, Tian Gao, Paul E. Staswick, Hongfeng Yu, Gota Morota, Harkamal Walia Oct 2022

Rice Chalky Grain 5 Regulates Natural Variation For Grain Quality Under Heat Stress, Anil Kumar Nalini Chandran, Jaspreet Sandhu, Larissa Irvin, Puneet Paul, Balpreet K. Dhatt, Waseem Hussain, Tian Gao, Paul E. Staswick, Hongfeng Yu, Gota Morota, Harkamal Walia

Department of Agronomy and Horticulture: Faculty Publications

Heat stress occurring during rice (Oryza sativa) grain development reduces grain quality, which often manifests as increased grain chalkiness. Although the impact of heat stress on grain yield is well-studied, the genetic basis of rice grain quality under heat stress is less explored as quantifying grain quality is less tractable than grain yield. To address this, we used an image-based colorimetric assay (Red, R; and Green, G) for genome-wide association analysis to identify genetic loci underlying the phenotypic variation in rice grains exposed to heat stress. We found the R to G pixel ratio (RG) derived from mature …


The Enablement And Written Description Requirements Through The Lens Of The Federal Circuit's Actions (As Opposed To Its Words), Christopher M. Holman Oct 2022

The Enablement And Written Description Requirements Through The Lens Of The Federal Circuit's Actions (As Opposed To Its Words), Christopher M. Holman

Faculty Works

No abstract provided.


The Temporal Impulse Response Function In Infantile Nystagmus, Gal Avital, Felicia Kuperwaser, Andrew W Pountain, Keenan A Lacey, Erin E Zwack, Magdalena Podkowik, Bo Shopsin, Victor J Torres, Itai Yanai Oct 2022

The Temporal Impulse Response Function In Infantile Nystagmus, Gal Avital, Felicia Kuperwaser, Andrew W Pountain, Keenan A Lacey, Erin E Zwack, Magdalena Podkowik, Bo Shopsin, Victor J Torres, Itai Yanai

Faculty, Staff and Student Publications

Innate immune recognition of bacterial pathogens is a key determinant of the ensuing systemic response, and host or pathogen heterogeneity in this early interaction can impact the course of infection. To gain insight into host response heterogeneity, we investigate macrophage inflammatory dynamics using primary human macrophages infected with Group B Streptococcus. Transcriptomic analysis reveals discrete cellular states within responding macrophages, one of which consists of four sub-states, reflecting inflammatory activation. Infection with six additional bacterial species—Staphylococcus aureus, Listeria monocytogenes, Enterococcus faecalis, Yersinia pseudotuberculosis, Shigella flexneri, and Salmonella enterica—recapitulates these states, though at …


Program Review Report October 11, 2022, Angela Brock Oct 2022

Program Review Report October 11, 2022, Angela Brock

Program Review Committee

No abstract provided.


Approved Programs 10-11-22, Angela Brock Oct 2022

Approved Programs 10-11-22, Angela Brock

Programs Committee (PC)

No abstract provided.


Whole Genome Sequence Analysis Of Blood Lipid Levels In >66,000 Individuals, Margaret Sunitha Selvaraj, Xihao Li, Zilin Li, Akhil Pampana, David Y. Zhang, Joanne E. Curran, Ravi Duggirala, John Blangero, Juan M. Peralta, Harald Hh Goring, Michael Mahaney Oct 2022

Whole Genome Sequence Analysis Of Blood Lipid Levels In >66,000 Individuals, Margaret Sunitha Selvaraj, Xihao Li, Zilin Li, Akhil Pampana, David Y. Zhang, Joanne E. Curran, Ravi Duggirala, John Blangero, Juan M. Peralta, Harald Hh Goring, Michael Mahaney

School of Medicine Publications

Blood lipids are heritable modifiable causal factors for coronary artery disease. Despite well-described monogenic and polygenic bases of dyslipidemia, limitations remain in discovery of lipid-associated alleles using whole genome sequencing (WGS), partly due to limited sample sizes, ancestral diversity, and interpretation of clinical significance. Among 66,329 ancestrally diverse (56% non-European) participants, we associate 428M variants from deep-coverage WGS with lipid levels; ~400M variants were not assessed in prior lipids genetic analyses. We find multiple lipid-related genes strongly associated with blood lipids through analysis of common and rare coding variants. We discover several associated rare non-coding variants, largely at Mendelian lipid …


Amyloid Β, Lipid Metabolism, Basal Cholinergic System, And Therapeutics In Alzheimer's Disease, Victoria Campos-Peña, Pavel Pichardo-Rojas, Talía Sánchez-Barbosa, Emma Ortíz-Islas, Citlali Ekaterina Rodríguez-Pérez, Pedro Montes, Gerardo Ramos-Palacios, Daniela Silva-Adaya, Rafael Valencia-Quintana, Jorge Francisco Cerna-Cortes, Danira Toral-Rios Oct 2022

Amyloid Β, Lipid Metabolism, Basal Cholinergic System, And Therapeutics In Alzheimer's Disease, Victoria Campos-Peña, Pavel Pichardo-Rojas, Talía Sánchez-Barbosa, Emma Ortíz-Islas, Citlali Ekaterina Rodríguez-Pérez, Pedro Montes, Gerardo Ramos-Palacios, Daniela Silva-Adaya, Rafael Valencia-Quintana, Jorge Francisco Cerna-Cortes, Danira Toral-Rios

2020-Current year OA Pubs

The presence of insoluble aggregates of amyloid β (Aβ) in the form of neuritic plaques (NPs) is one of the main features that define Alzheimer's disease. Studies have suggested that the accumulation of these peptides in the brain significantly contributes to extensive neuronal loss. Furthermore, the content and distribution of cholesterol in the membrane have been shown to have an important effect on the production and subsequent accumulation of Aβ peptides in the plasma membrane, contributing to dysfunction and neuronal death. The monomeric forms of these membrane-bound peptides undergo several conformational changes, ranging from oligomeric forms to beta-sheet structures, each …


Mesoscale Structure-Function Relationships In Mitochondrial Transcriptional Condensates, Marina Feric, Azadeh Sarfallah, Furqan Dar, Dmitry Temiakov, Rohit V Pappu, Tom Misteli Oct 2022

Mesoscale Structure-Function Relationships In Mitochondrial Transcriptional Condensates, Marina Feric, Azadeh Sarfallah, Furqan Dar, Dmitry Temiakov, Rohit V Pappu, Tom Misteli

2020-Current year OA Pubs

In live cells, phase separation is thought to organize macromolecules into membraneless structures known as biomolecular condensates. Here, we reconstituted transcription in condensates from purified mitochondrial components using optimized in vitro reaction conditions to probe the structure-function relationships of biomolecular condensates. We find that the core components of the mt-transcription machinery form multiphasic, viscoelastic condensates in vitro. Strikingly, the rates of condensate-mediated transcription are substantially lower than in solution. The condensate-mediated decrease in transcriptional rates is associated with the formation of vesicle-like structures that are driven by the production and accumulation of RNA during transcription. The generation of RNA alters …


Coherent Enhancement Of Optical Remission In Diffusive Media, Nicholas Bender, Arthur Goetschy, Chia Wei Hsu, Hasan Yılmaz, Pablo Jara Palacios, Alexey Yamilov, Hui Cao Oct 2022

Coherent Enhancement Of Optical Remission In Diffusive Media, Nicholas Bender, Arthur Goetschy, Chia Wei Hsu, Hasan Yılmaz, Pablo Jara Palacios, Alexey Yamilov, Hui Cao

Physics Faculty Research & Creative Works

Remitted waves are used for sensing and imaging in diverse diffusive media from the Earth's crust to the human brain. Separating the source and detector increases the penetration depth of light, but the signal strength decreases rapidly, leading to a poor signal-to-noise ratio. Here, we show, experimentally and numerically, that wavefront shaping a laser beam incident on a diffusive sample enables an enhancement of remission by an order of magnitude at depths of up to 10 transport mean free paths. We develop a theoretical model which predicts the maximal remission enhancement. Our analysis reveals a significant improvement in the sensitivity …


Tinjauan Yuridis Pengesahan Anak Luar Kawin Yang Dicatatkan Setelah Ayah Dari Anak Meninggal Dunia (Studi Kasus: Penetapan Pengadilan Negeri Jakarta Pusat Nomor: 36/Pdt.P/2020/Pn.Jkt.Pst.), Devina Delfian Oct 2022

Tinjauan Yuridis Pengesahan Anak Luar Kawin Yang Dicatatkan Setelah Ayah Dari Anak Meninggal Dunia (Studi Kasus: Penetapan Pengadilan Negeri Jakarta Pusat Nomor: 36/Pdt.P/2020/Pn.Jkt.Pst.), Devina Delfian

Lex Patrimonium

The birth of a child from an unregistered polygamous marriage according to the prevailing laws is a juridical phenomenon that cannot be denied. As the part of legal events, unregistered polygamous marriages can bring further legal consequences to children born out of wedlock, which in law children born out of wedlock have a lower position than children born from legal marriages. Because of these differences, the law provides a solution for children born out of wedlock to have the same legal status as children born from legal marriages, namely through child legalization. This research will be continued by focusing on …


Personality And Psychopathology Correlates Of Institutional Misconduct Among Juvenile Offenders, Paula Floyd Oct 2022

Personality And Psychopathology Correlates Of Institutional Misconduct Among Juvenile Offenders, Paula Floyd

Dissertations

Juvenile delinquency is a national concern, as delinquent behavior is associated with a host of poor psychosocial outcomes during later adolescence and adulthood. To address delinquency, it is important to understand psychological adjustment among adolescents who have already made contact with the juvenile justice system. One way to explore adjustment within this population involves examining personality and psychopathology correlates of institutional misconduct (i.e., behavioral infractions while incarcerated). While there is a robust body of literature regarding personality and psychopathology correlates of misconduct in adult inmate samples, there has been far less work devoted to these relationships among justice-involved youth. Furthermore, …


Observations On Late Cretaceous Micrampulla (Corethrales, Bacillariophyceae) From The Campbell Plateau (Zealandia), Southwest Pacific Ocean, Kenta Abe, David M. Harwood, Richard W. Jordan Oct 2022

Observations On Late Cretaceous Micrampulla (Corethrales, Bacillariophyceae) From The Campbell Plateau (Zealandia), Southwest Pacific Ocean, Kenta Abe, David M. Harwood, Richard W. Jordan

Department of Earth and Atmospheric Sciences: Faculty Publications

Late Cretaceous (late Campanian) diatom assemblages from the Campbell Plateau (Zealandia), southwest Pacific Ocean, obtained from Deep Sea Drilling Project (DSDP) Leg 29 Site 275, contain well-preserved specimens of two enigmatic diatom species currently assigned to the genus Ktenodiscus; Micrampulla parvula originally described from the Maastrichtian-age Moreno Shale, California, and Pterotheca cretacea from DSDP Site 275. In general, the two species share a number of common features with modern Corethron (domed valves, probable heterovalvate frustules, T-shaped serrated articulated spines, marginal sockets), but differ in the location of the sockets (i.e. vertically at the base of the valve dome and …


Alfalfa Water Productivity And Yield Gaps In The U.S. Central Great Plains, Kaylin P. Fink, Patricio Grassini, Alexandre Rocateli, Leonardo M. Bastos, Jude Kastens, Luke P. Ryan, Xiaomao Lin, Andres Patrignani, Romulo P. Lollato Oct 2022

Alfalfa Water Productivity And Yield Gaps In The U.S. Central Great Plains, Kaylin P. Fink, Patricio Grassini, Alexandre Rocateli, Leonardo M. Bastos, Jude Kastens, Luke P. Ryan, Xiaomao Lin, Andres Patrignani, Romulo P. Lollato

Department of Agronomy and Horticulture: Faculty Publications

Context: Yield gap (Yg) analyses using farmer-reported yield and management data have been performed for a number of annual grain crops, but it lacks for perennial forages. The U.S. accounts for 21 % of the global alfalfa production with a large rainfed area located in the central Great Plains, serving as an interesting case-study for Yg in perennial forages. Most existing alfalfa Yg analyses quantified the magnitude of the Yg but failed to identify associated management practices to reduce it. Challenging this analysis, a systematic benchmark for alfalfa water productivity [WP, kg dry matter per mm evapotranspiration (ETc)] that …


Effect Of Water Stress On Weed Germination, Growth Characteristics, And Seed Production: A Global Meta-Analysis, Mandeep Singh, Resham Thapa, Meetpal Singh Kukal, Suat Irmak, Steven Mirsky, Amit J. Jhala Oct 2022

Effect Of Water Stress On Weed Germination, Growth Characteristics, And Seed Production: A Global Meta-Analysis, Mandeep Singh, Resham Thapa, Meetpal Singh Kukal, Suat Irmak, Steven Mirsky, Amit J. Jhala

Department of Agronomy and Horticulture: Faculty Publications

Weeds compete with crops for soil moisture, along with other resources, which can impact the germination, growth, and seed production of weeds; however, this impact has not been systematically recorded and synthesized across diverse studies. To address this knowledge gap, a global meta-analysis was conducted using 1,196 paired observations from 86 published articles assessing the effect of water stress on weed germination, growth characteristics, and seed production. These studies were conducted and published during 1970 through 2020 across four continents (Asia, Australia, Europe, and North America). Imposed water stress was expressed as solution osmotic potential (ψsolution), soil water potential (ψsoil), …


Metabolome-Wide Associations Of Gestational Weight Gain In Pregnant Women With Overweight And Obesity, Jin Dai, Nansi S. Boghossian, Mark Sarzynski Ph.D., Faha, Facsm, Feng Luo, Xiaoqian Sun, Jian Li, Oliver Fiehn, Jihong Liu Sc.D., Liwei Chen Oct 2022

Metabolome-Wide Associations Of Gestational Weight Gain In Pregnant Women With Overweight And Obesity, Jin Dai, Nansi S. Boghossian, Mark Sarzynski Ph.D., Faha, Facsm, Feng Luo, Xiaoqian Sun, Jian Li, Oliver Fiehn, Jihong Liu Sc.D., Liwei Chen

Faculty Publications

Excessive gestational weight gain (GWG) is associated with adverse pregnancy outcomes. This metabolome-wide association study aimed to identify metabolomic markers for GWG. This longitudinal study included 39 Black and White pregnant women with a prepregnancy body mass index (BMI) of ≥ 25 kg/m2. Untargeted metabolomic profiling was performed using fasting plasma samples collected at baseline (mean: 12.1 weeks) and 32 weeks of gestation. The associations of metabolites at each time point and changes between the two time points with GWG were examined by linear and least absolute shrinkage and selection operator (LASSO) regression analyses. Pearson correlations between the …


Executive Committee - Agenda 10/11/2022, Academic Senate Oct 2022

Executive Committee - Agenda 10/11/2022, Academic Senate

Academic Senate Agendas

No abstract provided.


Role Of Stem Cell Transplant In Cd30+ Ptcl Following Frontline Brentuximab Vedotin Plus Chp Or Chop In Echelon-2., Kerry J Savage, Steven M Horwitz, Ranjana Advani, Jacob Haaber Christensen, Eva Domingo-Domenech, Giuseppe Rossi, Franck Morschhauser, Onder Alpdogan, Cheolwon Suh, Kensei Tobinai, Andrei Shustov, Marek Trneny, Sam Yuen, Pier Luigi Zinzani, Lorenz Trümper, Tim Ilidge, Owen A O'Connor, Barbara Pro, Harry Miao, Veronica Bunn, Keenan Fenton, Michelle Fanale, Markus Puhlmann, Swaminathan Iyer Oct 2022

Role Of Stem Cell Transplant In Cd30+ Ptcl Following Frontline Brentuximab Vedotin Plus Chp Or Chop In Echelon-2., Kerry J Savage, Steven M Horwitz, Ranjana Advani, Jacob Haaber Christensen, Eva Domingo-Domenech, Giuseppe Rossi, Franck Morschhauser, Onder Alpdogan, Cheolwon Suh, Kensei Tobinai, Andrei Shustov, Marek Trneny, Sam Yuen, Pier Luigi Zinzani, Lorenz Trümper, Tim Ilidge, Owen A O'Connor, Barbara Pro, Harry Miao, Veronica Bunn, Keenan Fenton, Michelle Fanale, Markus Puhlmann, Swaminathan Iyer

Department of Medical Oncology Faculty Papers

Peripheral T-cell lymphomas (PTCLs) are a heterogeneous group of aggressive non-Hodgkin lymphomas, the majority of which have high relapse rates following standard therapy. Despite use of consolidative stem cell transplant (SCT) following frontline therapy, there remains no consensus on its utility. The double-blind randomized phase 3 ECHELON-2 study (#NCT01777152; clinicaltrials.gov) demonstrated improved progression-free survival (PFS) and overall survival with frontline brentuximab vedotin plus cyclophosphamide, doxorubicin, and prednisone (A+CHP). Herein, we conducted an exploratory subgroups analysis of the impact of consolidative SCT on PFS in patients with previously untreated CD30+ PTCL (ALK- anaplastic large cell lymphoma [ALCL] and non-ALCL) …


Whole Genome Sequence Analysis Of Blood Lipid Levels In >66,000 Individuals, Margaret Sunitha Selvaraj, Xihao Li, Zilin Li, Akhil Pampana, David Y Zhang, Joseph Park, Stella Aslibekyan, Joshua C Bis, Jennifer A Brody, Brian E Cade, Lee-Ming Chuang, Ren-Hua Chung, Joanne E Curran, Lisa De Las Fuentes, Paul S De Vries, Ravindranath Duggirala, Barry I Freedman, Mariaelisa Graff, Xiuqing Guo, Nancy Heard-Costa, Bertha Hidalgo, Chii-Min Hwu, Marguerite R Irvin, Tanika N Kelly, Brian G Kral, Leslie Lange, Xiaohui Li, Martin Lisa, Steven A Lubitz, Ani W Manichaikul, Preuss Michael, May E Montasser, Alanna C Morrison, Take Naseri, Jeffrey R O'Connell, Nicholette D Palmer, Patricia A Peyser, Muagututia S Reupena, Jennifer A Smith, Xiao Sun, Kent D Taylor, Russell P Tracy, Michael Y Tsai, Zhe Wang, Yuxuan Wang, Wei Bao, John T Wilkins, Lisa R Yanek, Wei Zhao, Donna K Arnett, John Blangero, Eric Boerwinkle, Donald W Bowden, Yii-Der Ida Chen, Adolfo Correa, L Adrienne Cupples, Susan K Dutcher, Patrick T Ellinor, Myriam Fornage, Stacey Gabriel, Soren Germer, Richard Gibbs, Jiang He, Robert C Kaplan, Sharon L R Kardia, Ryan Kim, Charles Kooperberg, Ruth J F Loos, Karine A Viaud-Martinez, Rasika A Mathias, Stephen T Mcgarvey, Braxton D Mitchell, Deborah Nickerson, Kari E North, Bruce M Psaty, Susan Redline, Alexander P Reiner, Ramachandran S Vasan, Stephen S Rich, Cristen Willer, Jerome I Rotter, Daniel J Rader, Xihong Lin, Gina M Peloso, Pradeep Natarajan Oct 2022

Whole Genome Sequence Analysis Of Blood Lipid Levels In >66,000 Individuals, Margaret Sunitha Selvaraj, Xihao Li, Zilin Li, Akhil Pampana, David Y Zhang, Joseph Park, Stella Aslibekyan, Joshua C Bis, Jennifer A Brody, Brian E Cade, Lee-Ming Chuang, Ren-Hua Chung, Joanne E Curran, Lisa De Las Fuentes, Paul S De Vries, Ravindranath Duggirala, Barry I Freedman, Mariaelisa Graff, Xiuqing Guo, Nancy Heard-Costa, Bertha Hidalgo, Chii-Min Hwu, Marguerite R Irvin, Tanika N Kelly, Brian G Kral, Leslie Lange, Xiaohui Li, Martin Lisa, Steven A Lubitz, Ani W Manichaikul, Preuss Michael, May E Montasser, Alanna C Morrison, Take Naseri, Jeffrey R O'Connell, Nicholette D Palmer, Patricia A Peyser, Muagututia S Reupena, Jennifer A Smith, Xiao Sun, Kent D Taylor, Russell P Tracy, Michael Y Tsai, Zhe Wang, Yuxuan Wang, Wei Bao, John T Wilkins, Lisa R Yanek, Wei Zhao, Donna K Arnett, John Blangero, Eric Boerwinkle, Donald W Bowden, Yii-Der Ida Chen, Adolfo Correa, L Adrienne Cupples, Susan K Dutcher, Patrick T Ellinor, Myriam Fornage, Stacey Gabriel, Soren Germer, Richard Gibbs, Jiang He, Robert C Kaplan, Sharon L R Kardia, Ryan Kim, Charles Kooperberg, Ruth J F Loos, Karine A Viaud-Martinez, Rasika A Mathias, Stephen T Mcgarvey, Braxton D Mitchell, Deborah Nickerson, Kari E North, Bruce M Psaty, Susan Redline, Alexander P Reiner, Ramachandran S Vasan, Stephen S Rich, Cristen Willer, Jerome I Rotter, Daniel J Rader, Xihong Lin, Gina M Peloso, Pradeep Natarajan

Faculty, Staff and Student Publications

Blood lipids are heritable modifiable causal factors for coronary artery disease. Despite well-described monogenic and polygenic bases of dyslipidemia, limitations remain in discovery of lipid-associated alleles using whole genome sequencing (WGS), partly due to limited sample sizes, ancestral diversity, and interpretation of clinical significance. Among 66,329 ancestrally diverse (56% non-European) participants, we associate 428M variants from deep-coverage WGS with lipid levels; ~400M variants were not assessed in prior lipids genetic analyses. We find multiple lipid-related genes strongly associated with blood lipids through analysis of common and rare coding variants. We discover several associated rare non-coding variants, largely at Mendelian lipid …


Analysis Of Hybrid Learning For Students With Learning Disabilities In Primary Schools Providing Inclusive Education, Nugraheni Rachmawati, Asep Supena, Yufiarti Yufiarti, Gusti Yarmi, Asep Rudi Casmana Oct 2022

Analysis Of Hybrid Learning For Students With Learning Disabilities In Primary Schools Providing Inclusive Education, Nugraheni Rachmawati, Asep Supena, Yufiarti Yufiarti, Gusti Yarmi, Asep Rudi Casmana

The Qualitative Report

Many special-needs children, including students with learning disabilities, are enrolled in public schools. The current state of education is transitioning from online to face-to-face learning due to the improving situation after the COVID-19 pandemic. Hybrid learning is one of the alternative methods applied during the transition period. Therefore, this study aims to explore the experiences of teachers in implementing hybrid learning in students with disabilities in Indonesia using a qualitative description. Data was collected through interviews with one class teacher, two students with learning disabilities and their parents, and one school principal. Furthermore, observations were made on the learning process …


A Snapshot Of Science Education During Covid-19 In The Spring Of 2021, Lily Bentley Oct 2022

A Snapshot Of Science Education During Covid-19 In The Spring Of 2021, Lily Bentley

The Qualitative Report

The COVID-19 pandemic has placed many unique challenges on our education system. Unpacking the many issues that educators faced will allow researchers to understand some of the impacts that resulted from this unique phenomenon. This exploratory qualitative research study sought to understand how science educators and administrators made sense of science instruction during the spring of 2021. Data was collected through semi-structured interviews and online observations with ten K-12 science teachers and four administrators across two different counties within Virginia. Thematic coding was employed to analyze the findings, and results were validated through member checking with participants. Participants shared that …


Nationwide Public Perceptions Regarding The Acceptance Of Using Wastewater For Community Health Monitoring In The United States., A. Scott Lajoie, Asli Aslan, Rochelle H. Holm, Lauren B. Anderson, Heather D. Ness, Ted Smith Oct 2022

Nationwide Public Perceptions Regarding The Acceptance Of Using Wastewater For Community Health Monitoring In The United States., A. Scott Lajoie, Asli Aslan, Rochelle H. Holm, Lauren B. Anderson, Heather D. Ness, Ted Smith

Faculty and Staff Scholarship

To assess the levels of infection across communities during the coronavirus disease 2019 pandemic, researchers have measured severe acute respiratory syndrome coronavirus 2 RNA in feces dissolved in sewer water. This activity is colloquially known as sewer monitoring and is referred to as wastewater-based epidemiology in academic settings. Although global ethical principles have been described, sewer monitoring is unregulated for health privacy protection when used for public health surveillance in the United States. This study used Qualtrics XM, a national research panel provider, to recruit participants to answer an online survey. Respondents (N = 3,083) answered questions about their knowledge, …


Mesoscale Structure-Function Relationships In Mitochondrial Transcriptional Condensates, Marina Feric, Azadeh Sarfallah, Furqan Dar, Dmitry Temiakov, Rohit V. Pappu, Tom Misteli Oct 2022

Mesoscale Structure-Function Relationships In Mitochondrial Transcriptional Condensates, Marina Feric, Azadeh Sarfallah, Furqan Dar, Dmitry Temiakov, Rohit V. Pappu, Tom Misteli

Department of Biochemistry and Molecular Biology Faculty Papers

In live cells, phase separation is thought to organize macromolecules into membraneless structures known as biomolecular condensates. Here, we reconstituted transcription in condensates from purified mitochondrial components using optimized in vitro reaction conditions to probe the structure-function relationships of biomolecular condensates. We find that the core components of the mt-transcription machinery form multiphasic, viscoelastic condensates in vitro. Strikingly, the rates of condensate-mediated transcription are substantially lower than in solution. The condensate-mediated decrease in transcriptional rates is associated with the formation of vesicle-like structures that are driven by the production and accumulation of RNA during transcription. The generation of RNA alters …


Evolution And Modulation Of Antigen-Specific T Cell Responses In Melanoma Patients, Jani Huuhtanen, Liang Chen, Emmi Jokinen, Henna Kasanen, Tapio Lönnberg, Anna Kreutzman, Katriina Peltola, Micaela Hernberg, Chunlin Wang, Cassian Yee, Harri Lähdesmäki, Mark M Davis, Satu Mustjoki Oct 2022

Evolution And Modulation Of Antigen-Specific T Cell Responses In Melanoma Patients, Jani Huuhtanen, Liang Chen, Emmi Jokinen, Henna Kasanen, Tapio Lönnberg, Anna Kreutzman, Katriina Peltola, Micaela Hernberg, Chunlin Wang, Cassian Yee, Harri Lähdesmäki, Mark M Davis, Satu Mustjoki

Faculty, Staff and Student Publications

Analyzing antigen-specific T cell responses at scale has been challenging. Here, we analyze three types of T cell receptor (TCR) repertoire data (antigen-specific TCRs, TCR-repertoire, and single-cell RNA + TCRαβ-sequencing data) from 515 patients with primary or metastatic melanoma and compare it to 783 healthy controls. Although melanoma-associated antigen (MAA) -specific TCRs are restricted to individuals, they share sequence similarities that allow us to build classifiers for predicting anti-MAA T cells. The frequency of anti-MAA T cells distinguishes melanoma patients from healthy and predicts metastatic recurrence from primary melanoma. Anti-MAA T cells have stem-like properties and frequent interactions with regulatory …