Digital Commons Network^™

A Pilot Research Project To Enhance Inquiry-Based Learning By Mapping The Microbiome Of The Southern Appalachian Region, Shivam Patel

Tennessee Posters at the Capitol [2022]

As humans continue to advance healthcare resources, we face a growing threat of nosocomial multidrug-resistant bacteria. The rise of these antibiotic-resistant microorganisms has been placed on the World Health Organization’s watchlist as one of the biggest threats to global health. We continue to have a shortage of effective antibiotics with the rise of these “superbugs”. With the growing number of deadly pathogens, the future of medicine relies on scientific findings to combat multidrug-resistant bacteria. Appalachia could be the answer to combat this new health threat. As the most biodiverse temperate forest region in North America, our beautiful backyard in the …

Long Term Estrogen Loss Worsens Heart Function In Aged Female Mice, Pamela Avendaño-Rubí

Tennessee Posters at the Capitol [2022]

Heart disease is the leading cause of death worldwide and according to the American Heart Association, the risk of HD in aging menopausal women doubles compared to men of the same age. Excessive contractility of blood vessels is a common feature in heart disease. Clinical and animal studies further support that estrogen loss worsen the contractility in the heart but the details remain unclear. Thus, the overall goal of this work was to examine how the timing and duration of estrogen loss affects heart failure. Our hypothesis is that long-term estrogen loss following heart failure worsens cardiac function of the …

Biomechanical Evaluation Of Recurrent Dissociation Of Modular Humeral Prostheses, Daniel B. Luckenbill, Mike F. Iossi, Alyssa M. George Whitney, Danielle Miller, Lynn A. Crosby, Tarun Goswami

Biomedical, Industrial & Human Factors Engineering Faculty Publications

The purpose of the study was to evaluate the force and torque required to dissociate a humeral head from the unimplanted modular total shoulder replacement system from different manufacturers and to determine if load and torque to dissociation are reduced in the presence of bodily fluids. Impingement, taper contamination, lack of compressive forces, and interference of taper fixation by the proximal humerus have all been reported as possible causes for dissociation. Experimental values determined in this research were compared with literature estimates of dissociation force of the humeral head under various conditions to gain more understanding of the causes of …

Qualitative/Covid Journals, Judy Schmitt

Library Faculty Works

No abstract provided.

Defining Sex, Edward Schiappa

Law and Contemporary Problems

No abstract provided.

Sex Neutrality, Doriane Lambelet Coleman

Law and Contemporary Problems

No abstract provided.

The Gatekeepers Of Research: Why A Data Protection Authority Holds The Key To Research In The New York Privacy Acts, Eric B. Green

Brooklyn Law Review

Biometric data is among the most sensitive of personal data because it is biologically tied and unique to the individual. Nonetheless, biometric data is an invaluable facet of the research that enables progressive scientific, technological, and medical innovation. Because a comprehensive federal data privacy act does not appear to be on the horizon, the torch has been passed to the states to create their own personal data protection regimes. New Yorkers’ personal biometric data is not aptly protected, partially because neither the New York Privacy Act nor the Biometric Privacy Act (collectively, the NY Privacy Acts) have matured to the …

Long-Term Monitoring And Maintenance Plan For The Mixed Waste Landfill - March 2012, Revision I February 16, 2022, Sandia National Laboratories/Nm

Facilities-Units

This Revision I_February 16, 2022 version of the Long-term Monitoring and Maintenance Plan for the Mixed Waste Landfill replaces the original March, 2012 MWL LTMMP.

The Lumberjack, February 16, 2022

Cal Poly Humboldt's Student Newspaper

The student newspaper of Humboldt State University.

Effects Of Specific Blocking Buffers On Histone H2b Antibodies, Noha Al-Saadi, Grace Mix, Mardasia Thompson, Elin Zaman

JSU Student Symposium 2022

Mammalian histones have been known to be one of the most highly alkaline proteins found in the nuclei. Their main function is to organize DNA into chromosomes and regulate transcription. An example of a histone would be Histone H2B, which this project focuses heavily on. Histone H2B has been seen to bind poorly to antibodies and result in a weak signal. The goal of this project is to test a multitude of blocking buffers, in triplicate, in order to find a blocking buffer that can obtain the strongest signal from the antibodies being tested. Through the use of protein gel …

Antioxidant Content In Plant-Based Diets Versus Meat-Based Diets, Lia Meadows, Peeper Walker, Reveca Tomas

JSU Student Symposium 2022

The result of naturally occurring free radicals in the body are oxidative-stress-related damages to cells, tissues, and biomolecules. Oxidative stress occurs when the balance between reactive oxygen species (ROS) outnumbers antioxidants, producing negative effects. Antioxidants exist as a natural defense mechanism utilized by the body. This defense exists in two distinct forms: enzymatic and non-enzymatic antioxidants. Non-enzymatic antioxidants consist of vitamins and bioactive compounds, such as phenols. In previous studies, plant-based diets showed promising results in reducing damage caused by oxidative-stress. The purpose of this study was to determine the extent to which antioxidant concentration differs between meat-based diet and …

Genetic Assessment Of Congenital Stationary Night Blindness-Associated Trpm1 Variants Of Uncertain Significance In C. Elegans, Sara Morris

JSU Student Symposium 2022

Congenital stationary night blindness (CSNB) is a disorder of the eye that impacts the ability to see in low to no light conditions. It occurs in horses and humans. TRPM1 encodes for a member of the transient receptor potential melastatin subfamily of transient receptor potential ion channels. Mutations in TRPM1 have been identified by previous researchers in horses with leopard complex pattern (LCP) suffering also from CSNB. Phenotypic changes in these horses are a loss of spotted coat pattern. An attempt was made to locate variants of uncertain significance (VUS) in TRPM1 that had been previously identified in horses with …

A Split Nanoluc Reporter Quantitatively Measures Circular Rna Ires Translation, Gabriele Fuchs, Priyanka Sehta, Ann-Marie Willhelm, Shu-Jun Lin, Michelle A. Urman, Haley A. Macneil

Biological Sciences Faculty Scholarship

Internal ribosomal entry sites (IRESs) are RNA secondary structures that mediate translation independent from the m7G RNA cap. The dicistronic luciferase assay is the most frequently used method to measure IRES-mediated translation. While this assay is quantitative, it requires numerous controls and can be time-consuming. Circular RNAs generated by splinted ligation have been shown to also accurately report on IRES-mediated translation, however suffer from low yield and other challenges. More recently, cellular sequences were shown to facilitate RNA circle formation through backsplicing. Here, we used a previously published backsplicing circular RNA split GFP reporter to create a highly sensitive and …

Reactive Oxygen Species In Plant Interactions With Aphids, Fiona L. Goggins, Hilary D. Fischer

Entomology Faculty Publications and Presentations

Reactive oxygen species (ROS) such as hydrogen peroxide and superoxide are produced in plants in response to many biotic and abiotic stressors, and they can enhance stress adaptation in certain circumstances or mediate symptom development in others. The roles of ROS in plant-pathogen interactions have been extensively studied, but far less is known about their involvement in plant-insect interactions. A growing body of evidence, however, indicates that ROS accumulate in response to aphids, an economically damaging group of phloem-feeding insects. This review will cover the current state of knowledge about when, where, and how ROS accumulate in response to aphids, …

Organizing And Arguing Sex And Gender, Anne B. Goldstein

Law and Contemporary Problems

No abstract provided.

Board Diversity: People Or Pathways?, June Carbone

Law and Contemporary Problems

No abstract provided.

Phylogenomic Discordance Suggests Polytomies Along The Backbone Of The Large Genus Solanum, Edeline Gagnon, Rebeccca Hilgenhof, Andrés Orejuela, Angela J. Mcdonnell, Gaurav Sablok, Xavier Aubriot, Leandro Giacomin, Yuri Gouvêa, Thamyris Bragionis, João Renato Stehmann, Lynn Bohs, Steven Dodsworth, Christopher T. Martine, Péter Poczai, Sandra Knapp, Tiina Särkinen

Faculty Journal Articles

Premise of the study

Evolutionary studies require solid phylogenetic frameworks, but increased volumes of phylogenomic data have revealed incongruent topologies among gene trees in many organisms both between and within genomes. Some of these incongruences indicate polytomies that may remain impossible to resolve. Here we investigate the degree of gene-tree discordance in Solanum, one of the largest flowering plant genera that includes the cultivated potato, tomato, and eggplant, as well as 24 minor crop plants.

Methods

A densely sampled species-level phylogeny of Solanum is built using unpublished and publicly available Sanger sequences comprising 60% of all accepted species (742 spp.) …

Allosteric Determinants Of The Sars-Cov-2 Spike Protein Binding With Nanobodies: Examining Mechanisms Of Mutational Escape And Sensitivity Of The Omicron Variant, Gennady M. Verkhivker

Mathematics, Physics, and Computer Science Faculty Articles and Research

Structural and biochemical studies have recently revealed a range of rationally engineered nanobodies with efficient neutralizing capacity against the SARS-CoV-2 virus and resilience against mutational escape. In this study, we performed a comprehensive computational analysis of the SARS-CoV-2 spike trimer complexes with single nanobodies Nb6, VHH E, and complex with VHH E/VHH V nanobody combination. We combined coarse-grained and all-atom molecular simulations and collective dynamics analysis with binding free energy scanning, perturbation-response scanning, and network centrality analysis to examine mechanisms of nanobody-induced allosteric modulation and cooperativity in the SARS-CoV-2 spike trimer complexes with these nanobodies. By quantifying energetic and allosteric …

Integrating Natural Language Processing And Interpretive Thematic Analyses To Gain Human-Centered Design Insights On Hiv Mobile Health: Proof-Of-Concept Analysis, Simone J. Skeen, Stephen Scott Jones, Carolyn Marie Cruse, Keith J. Horvath

Publications and Research

Background: HIV mobile health (mHealth) interventions often incorporate interactive peer-to-peer features. The user-generated content (UGC) created by these features can offer valuable design insights by revealing what topics and life events are most salient for participants, which can serve as targets for subsequent interventions. However, unstructured, textual UGC can be difficult to analyze. Interpretive thematic analyses can preserve rich narratives and latent themes but are labor-intensive and therefore scale poorly. Natural language processing (NLP) methods scale more readily but often produce only coarse descriptive results. Recent calls to advance the field have emphasized the untapped potential of combined NLP …

Caregivers Need Care, Too: Conceptualising Spiritual Care For Migrant Caregivers-Transnational Mothers, Ma. Adeinev M. Reyes-Espiritu

Theology Department Faculty Publications

Growing research revolving around the plight of (Philippine) migrant domestic workers is noteworthy. However, the focus is largely on their role, capacity and identity as caregivers, meaning as labour migrants and transnational mothers engaged in both paid and unpaid care work. Building on the “care circulation” framework of Baldassar and Merla that conceptualises care as given and received in varying degrees by all family members across time and distance, this paper takes up the task of recognising migrant domestic workers as care receivers. In a particular way, this paper conceptualises care for migrant caregivers-transnational mothers that is based on a …

Foreword, Doriane Lambelet Coleman, Kimberly D. Krawiec

Law and Contemporary Problems

No abstract provided.

The Importance Of Referring To Human Sex In Language, Kathleen Stock

Law and Contemporary Problems

No abstract provided.

A Current Model Of Sex Including All Biological Components Of Sexual Reproduction, Joshua D. Safer

Law and Contemporary Problems

No abstract provided.

Unlawful Genders, Anthony Michael Kreis

Law and Contemporary Problems

No abstract provided.

Stall Wars: Sex And Civil Rights In The Public Bathroom, Wickliffe Shreve

Law and Contemporary Problems

No abstract provided.

Feminism, Trans Justice, And Speech Rights: A Comparative Perspective, Madeleine Pape

Law and Contemporary Problems

No abstract provided.

Hearing And Vestibular Loss With Misuse Of Opioids And Illicit Drugs: A Review Of The Literature, Michelle L. Hughes, Amanda Rodriquez, Jonathan Hatch, Kenneth Zoucha

Department of Special Education and Communication Disorders: Faculty Publications

Background: The purpose of this review was to summarize the literature regarding the effects of opioids and illicit drugs on the auditory and vestibular systems.

Methods: Data were sourced from published papers reporting hearing loss (HL) and/or vestibular loss (VL) following misuse or overdose of opioids or illicit drugs. Most papers consisted of retrospective single-case reports, with few retrospective reviews or prospective cohort studies. Search terms included variations of HL, VL, opioids, and illicit drugs. Search results yielded 51 articles published between 1976 and 2021. A total of 44 articles were reviewed after excluding studies that were not available in …

Absence Of An Association Between Macular Degeneration And Young-Onset Dementia, Tung-Mei Tammy Kuang, Sudha Xirasagar Mbbs., Ph.D., Wei-Yun Lee, Yen-Fu Cheng, Nai-Wen Kuo, Herng-Ching Lin

Faculty Publications

A few population-based studies have reported an association between prior age-related macular degeneration and senile dementia. No study has explored a possible link between prior macular degeneration and young-onset dementia (YOD). This case–control study aimed to evaluate the association of YOD with prior macular degeneration diagnosed in the 5-year period before their index date. Data for this retrospective observational study were retrieved from Taiwan’s National Health Insurance (NHI) dataset. A total of 36,577 patients with newly diagnosed YOD from January 2010 to December 2017 were identified as the study cohort, assigning their diagnosis date as their index date. Comparison patients …

Humpty Dumpty Derangement Syndrome, Jethro K. Lieberman

Other Publications

No abstract provided.

Feasibility Of Administering Human Pancreatic Cancer Chemotherapy In A Spontaneous Pancreatic Cancer Mouse Model, Abagail M Delahoussaye, Joseph Abi Jaoude, Morgan Green, Tara N Fujimoto, Jessica Molkentine, Carolina J Garcia Garcia, Jason P Gay, Ningping Feng, Joseph Marszalek, Natalie Fowlkes, Cullen M Taniguchi

Faculty, Staff and Student Publications

Background: Both modified FOLFIRINOX (mFFX) and gemcitabine/nab-paclitaxel chemotherapy regimens have been shown to improve clinical outcomes in patients with pancreatic cancer, and are often used interchangeably as the standard of care. Preclinical studies often do not use these regimens, since administering these multiagent approaches can be difficult. In this study, we assessed the feasibility of administering these two chemotherapy regimens in spontaneous pancreatic tumors using KPC mice with the ultimate goal of advancing preclinical studies.

Methods: KPC mice were created by breeding KrasLSL-G12D/+ to Trp53fl/fl;Ptf1αCre/+, resulting in KrasLSL-G12D/+;p53fl/+;Ptf1αCre/+ mice. At 14 weeks of age, mice were palpated for spontaneous tumor …