Digital Commons Network^™

Microbial Feedbacks On Soil Organic Matter Dynamics Underlying The Legacy Effect Of Diversified Cropping Systems, Maria Mooshammer, A. Stuart Grandy, Francisco Calderon, Steve Culman, Bill Deen, Rhae A. Drijber, Kari Dunfield, Virginia L. Jin, R. Michael Lehman, Shannon L. Osborne, Marty Schmer, Timothy M. Bowles

Faculty Publications

Crop rotations have well-known aboveground and belowground benefits. At regional to continental scales, the unifying mechanisms of how diversified rotations alter soil organic matter (SOM) dynamics have not been demonstrated. We assessed how increasing crop rotational diversity across a soil-climate gradient affected the integrated response of SOM chemistry, microbial community composition, and its enzymatic potential to degrade SOM. Agroecosystems with the same crop rotational diversity (all sampled during the corn phase) shared similarities in molecular SOM patterns with a strong microbial signature, pointing to common transformation processes. Differences in SOM chemistry between rotations were mainly characterized by shifts in microbial …

Late-Stage Chemoenzymatic Installation Of Hydroxy-Bearing Allyl Moiety On The Indole Ring Of Tryptophan-Containing Peptides, Nagaraju Mupparapu, Lauren Brewster, Katrina F. Ostrom, Sherif I. Elshahawi

Pharmacy Faculty Articles and Research

The late-stage functionalization of indole- and tryptophan-containing compounds with reactive moieties facilitates downstream diversification and leads to changes in their biological properties. Here, the synthesis of two hydroxy-bearing allyl pyrophosphates is described. A chemoenzymatic method is demonstrated which uses a promiscuous indole prenyltransferase enzyme to install a dual reactive hydroxy-bearing allyl moiety directly on the indole ring of tryptophan-containing peptides. This is the first report of late-stage indole modifications with this reactive group.

Repair-Assisted Damage Detection Reveals Biological Disparities In Prostate Cancer Between African Americans And European Americans, Kimiko L Krieger, Jie H Gohlke, Kevin J Lee, Danthasinghe Waduge Badrajee Piyarathna, Patricia D Castro, Jeffrey A Jones, Michael M Ittmann, Natalie R Gassman, Arun Sreekumar

Faculty, Staff and Students Publications

African Americans (AA) are two times more likely to be diagnosed with and succumb to prostate cancer (PCa) compared to European Americans (EA). There is mounting evidence that biological differences in these tumors contribute to disparities in patient outcomes. Our goal was to examine the differences in DNA damage in AA and EA prostate tissues. Tissue microarrays with matched tumor-benign adjacent pairs from 77 AA and EA PCa patients were analyzed for abasic sites, oxidative lesions, crosslinks, and uracil content using the Repair Assisted Damage Detection (RADD) assay. Our analysis revealed that AA PCa, overall, have more DNA damage than …

The Colby Echo (2022-02-17), Colby College

The Colby Echo

Published by the students of Colby College since 1877, The Colby Echo is the weekly, editorially independent student-run newspaper of Colby College in Waterville, Maine. Published monthly, 1877-1886; semi-monthly; 1886-1897; and weekly during the academic year, 1898-present.

Chemical Reversible Crosslinking Enables Measurement Of Rna 3d Distances And Alternative Conformations In Cells, Ryan Van Damme, Kongpan Li, Minjie Zhang, Jianhui Bai, Wilson H. Lee, Joseph D. Yesselman, Zhipeng Lu, Willem A. Velema

Department of Chemistry: Faculty Publications

Three-dimensional (3D) structures dictate the functions of RNA molecules in a wide variety of biological processes. However, direct determination of RNA 3D structures in vivo is difficult due to their large sizes, conformational heterogeneity, and dynamics. Here we present a method, Spatial 2′-Hydroxyl Acylation Reversible Crosslinking (SHARC), which uses chemical crosslinkers of defined lengths to measure distances between nucleotides in cellular RNA. Integrating crosslinking, exonuclease (exo) trimming, proximity ligation, and high throughput sequencing, SHARC enables transcriptome-wide tertiary structure contact maps at high accuracy and precision, revealing heterogeneous RNA structures and interactions. SHARC data provide constraints that improves Rosetta-based RNA 3D …

Improving Taxonomic Delimitation Of Fungal Species In The Age Of Genomics And Phenomics, Asjley Stengel, Kimberly M. Stanke, Amanda C. Quattrone, Joshua Herr

Department of Plant Pathology: Faculty Publications

Species concepts have long provided a source of debate among biologists. These lively debates have been important for reaching consensus on how to communicate across scientific disciplines and for advancing innovative strategies to study evolution, population biology, ecology, natural history, and disease epidemiology. Species concepts are also important for evaluating variability and diversity among communities, understanding biogeographical distributions, and identifying causal agents of disease across animal and plant hosts. While there have been many attempts to address the concept of species in the fungi, there are several concepts that have made taxonomic delimitation especially challenging. In this review we discuss …

Aapm Medical Physics Practice Guideline 12.A: Fluoroscopy Dose Management, Ryan F. Fisher, Kimberly E. Applegate, Lindsey K. Berkowitz, Olav Christianson, Jaydev K. Dave, Lindsay Deweese, Nichole Harris, Mary Ellen Jafari, A. Kyle Jones, Robert J. Kobistek, Brendan Loughran, Loren Marous, Donald L. Miller, Beth Schueler, Bryan C. Schwarz, Adam Springer, Kevin A. Wunderle

Radiology Faculty Publications

No abstract provided.

The Antarctic Weddell Seal Genome Reveals Evidence Of Selection On Cardiovascular Phenotype And Lipid Handling, Hyun Ji Noh, Jason Turner-Maier, S. Anne Schulberg, Michael L. Fitzgerald, Jeremy Johnson, Kaitlin N. Allen, Luis A. Huckstadt, Annabelle J. Batten, Jessica Alfoldi, Daniel P. Costa, Elinor K. Karlsson, Warren M. Zapol, Emmanuel S. Buys, Kerstin Lindbald-Toh, Allyson G. Hindle

Life Sciences Faculty Research

The Weddell seal (Leptonychotes weddellii) thrives in its extreme Antarctic environment. We generated the Weddell seal genome assembly and a high-quality annotation to investigate genome-wide evolutionary pressures that underlie its phenotype and to study genes implicated in hypoxia tolerance and a lipid-based metabolism. Genome-wide analyses included gene family expansion/contraction, positive selection, and diverged sequence (acceleration) compared to other placental mammals, identifying selection in coding and non-coding sequence in five pathways that may shape cardiovascular phenotype. Lipid metabolism as well as hypoxia genes contained more accelerated regions in the Weddell seal compared to genomic background. Top-significant genes were SUMO2 and EP300; …

The Association Of Persistent Symptoms Of Depression And Anxiety With Recurrent Acute Coronary Syndrome Events: A Prospective Observational Study, Abdullah S. Alhurani, Ayman M. Hamdan-Mansour, Muayyad M. Ahmad, Gabrielle Mckee, Sharon O’Donnell, Frances O’Brien, Mary Mooney, Zyad T. Saleh, Debra K. Moser

Nursing Faculty Publications

The purpose of this study was to examine the role of persistent symptoms of depression and anxiety in a second acute coronary syndrome (ACS) event. Data presented in this study were from an RCT study. A follow-up for 24 months after baseline to detect a second ACS event among 1162 patients from five hospitals. Hierarchal Cox regression analyses were used. The results showed that persistent depression only (HR 2.27; 95% CI: 1.35–3.81; p = 0.002), and comorbid persistent depression and anxiety (HR 2.03; 95% CI: 1.03–3.98; p = 0.040) were the significant predictors of a second ACS event. Secondary education …

Novel Role Of Ghrelin Receptor In Gut Dysbiosis And Experimental Colitis In Aging, Ji Yeon Noh, Chia-Shan Wu, Jennifer A A Deluca, Sridevi Devaraj, Arul Jayaraman, Robert C Alaniz, Xiao-Di Tan, Clinton D Allred, Yuxiang Sun

Faculty, Staff and Students Publications

Chronic low-grade inflammation is a hallmark of aging, which is now coined as inflamm-aging. Inflamm-aging contributes to many age-associated diseases such as obesity, type 2 diabetes, cardiovascular disease, and inflammatory bowel disease (IBD). We have shown that gut hormone ghrelin, via its receptor growth hormone secretagogue receptor (GHS-R), regulates energy metabolism and inflammation in aging. Emerging evidence suggests that gut microbiome has a critical role in intestinal immunity of the host. To determine whether microbiome is an integral driving force of GHS-R mediated immune-metabolic homeostasis in aging, we assessed the gut microbiome profiles of young and old GHS-R global knockout …

Investigating How Ecologically Relevant Thermal Conditions Affect Gonadal Development In A Turtle With Temperature-Dependent Sex Determination, Anthony Trey Breitenbach

Theses and Dissertations

In turtles and numerous other reptilian species, sex is determined by the environmental temperatures during embryonic development, which is referred to temperature-dependent sex determination (TSD). In the type of TSD common in turtles, cool temperatures (below 29 °C) during embryonic development produce mainly males while warm temperatures (above 29 °C) produce mainly females. Gonadal differentiation in TSD species involves numerous genes that are also necessary to produce a testis or ovary in species with genotypic sex determination. However, most of what we know about TSD comes from studies performed in the laboratory using constant temperature incubation conditions, which are not …

2022-02-17 Faculty Senate Newsletter, Morehead State University. Faculty Senate.

Faculty Senate Records

Faculty Senate newsletter from February 17, 2022.

Draft Genome Sequencing Of Three Glutaraldehyde-Tolerant Bacteria From Produced Water From Hydraulic Fracturing, Stephen Techtmann, Andrew L. Baldwin, Dotun Aluko, Justin Andersen, Cole Becker, Grace Chandler, Steve Forgrave, Madelyn Jones, Ina Klasner, Jared Martini, Noah Mason, Ryleigh Parsons, Nick Peterson, Erik Reynolds, Lydia Schroeder

Michigan Tech Publications, Part 1

Here, we report the draft genome sequence of three glutaraldehyde-resistant isolates from produced water from hydraulic fracturing operations. The three strains were identified as sp. strain G11, sp. strain G15, and sp. strain G16. The genome sequences of these isolates will provide insights into biocide resistance in hydraulic fracturing operations.

Sustained Efficacy And Safety Of Burosumab, A Monoclonal Antibody To Fgf23, In Children With X-Linked Hypophosphatemia, Agnès Linglart, Erik A Imel, Michael P Whyte, Anthony A Portale, Wolfgang Högler, Annemieke M Boot, Raja Padidela, William Van't Hoff, Gary S Gottesman, Angel Chen, Alison Skrinar, Mary Scott Roberts, Thomas O Carpenter

2020-Current year OA Pubs

PURPOSE: In X-linked hypophosphatemia (XLH), excess fibroblast growth factor-23 causes hypophosphatemia and low calcitriol, leading to musculoskeletal disease with clinical consequences. XLH treatment options include conventional oral phosphate with active vitamin D, or monotherapy with burosumab, a monoclonal antibody approved to treat children and adults with XLH. We have previously reported outcomes up to 64 weeks, and here we report safety and efficacy follow-up results up to 160 weeks from an open-label, multicenter, randomized, dose-finding trial of burosumab for 5- to 12-year-old children with XLH.

METHODS: After 1 week of conventional therapy washout, patients were randomized 1:1 to burosumab every …

An Axiomatic And Contextual Review Of The Armitage And Doll Model Of Carcinogenesis, W. Zane Billings, Justin Clifton, Josh Hiller, Tommy Meek, Andrew Penland, Wesley Rogers, Gabriella Smokovich, Andrew Velasquez-Berroteran, Eleni Zamagias

Spora: A Journal of Biomathematics

In 1954, Armitage and Doll published one of the most influential papers in the history of mathematical epidemiology. However, when one examines the literature one finds that there are in fact at least three distinct mathematical models attributed to the 1954 paper. In this study, we examine this important paper and the mathematical derivation of their model. We find, very surprisingly, that no stochastic process can account for all the assumptions of the model and that many of the models in the literature use a consistent subset of the assumptions used in Armitage and Doll's paper.

Interview With George Rogers, Zach S. Henderson Library Special Collections

Zach S. Henderson Library Special Collections Oral History Collection

George Rogers interviewed by an unknown interviewer possibly affiliated with Georgia Heritage Trust, January 17, 1991. Find this collection in the University Libraries' catalog!

Benefits Of Chinese Qigong Mind-Body Exercise Program On Salivary Biomarkers And Physical Functions In Older Adults, Salmaan Faghihi, Ashley Nguyen, Karen Cruz, Mary Bahwawsi, Joseph Nocito, Zenong Yin, Yan Du, Tianou Zhang

International Journal of Exercise Science: Conference Proceedings

Chinese Qigong mind-body exercise such as Five Animal Frolics have been shown to improve quality of life, physical functions, cognitive functions, and mental health in older adults with various chronic health conditions. However, there is a lack of comprehensive understanding of the biological mechanisms, underlying the health effects after exposing to conventional and mind-body exercise. PURPOSE: The purpose of the study is to profile the changes associated with salivary biomarkers and physical functions after exposure to a 12-week five animal frolics, Chinese Qigong mind-body exercise program in community-dwelling older adults. METHODS: Thirteen community-dwelling adults who attended the Senior …

Males, But Not Females, Demonstrate Mitochondrial Dysfunction In The C26 Model Of Cancer Cachexia, Ricky D. Solis, J. William Deaver, Eleanor R. Schrems, Ana Regina Cabrera, Seongkyun Lim, Francielle Morena Da Silva, Tyrone A. Washington, Nicholas P. Greene, Michael P. Wiggs

International Journal of Exercise Science: Conference Proceedings

Cancer cachexia is characterized by progressive muscle wasting that can lead to symptoms such as anemia, severe weight loss, and fatigue. These symptoms can lead to limitations in activities of daily living and can cause resistance to chemotherapy treatments in cancer patients. There are no current treatments available to treat cancer cachexia and a critical need remains to identify mechanisms of cancer cachexia. Recently, our group identified mitochondrial disfunction precedes muscle atrophy in males but not females in a model of lung cancer induced atrophy. However, it is unknown whether this finding is replicated when studying a different type of …

Association Between Total Body Composition And Vo2max In Individuals With Mid-Spectrum Chronic Kidney Disease, Ricardo Torres, Katie Richardson, Jeffery L. Heileson, Ahmed Ismaeel, Andrew Gallucci, Panagiotis Koutakis, Rodney G. Bowden, Leslee Funderburk, Jeff S. Forsse

International Journal of Exercise Science: Conference Proceedings

Total body composition (TBC), a measure of body fat percentage (%BF), lean body mass (LBM), and bone mineral content (BMC), can be used as a predictor of cardiovascular fitness. Prior studies have established a relationship between TBC and VO2max in healthy individuals over 35 years of age. However, this relationship is poorly understood in chronic disease populations. PURPOSE: To assess the relationship between TBC and cardiovascular fitness in a clinical population of adults with mid-spectrum (stages G2, G3a, and G3b) chronic kidney disease (CKD). METHODS: A cross-sectional analysis was conducted among 24 subjects diagnosed with mid-spectrum CKD. Nine …

Autophagy, But Not Proteolysis, May Aid In Muscle Protein Synthesis, Megan H. Lewis, Patrick J. Ryan, Sean T. Stanelle, Colleen L. O'Reilly, Jessica M. Cardin, James D. Fluckey

International Journal of Exercise Science: Conference Proceedings

For muscle growth to occur, protein synthesis must be greater than protein degradation. However, up to this point, anabolic pathways have garnered the brunt of investigations examining anabolic capacity with little investigation into the connectedness of catabolic signaling on these anabolic targets. PURPOSE: The purpose of this study was to elucidate the contributions of proteasomal-dependent and autophagic-dependent catabolic pathways on anabolism via analysis of fractional synthetic rates (FSR) in L6 myotubes. METHODS: Differentiated, cultured L6 myoblasts were treated with media containing 4% deuterium oxide (stable isotope label) and a corresponding pharmacological treatment (NSC 185058 [autophagic inhibitor; 100 μM], …

Insulin-Induced Increase In Anabolic Capacity Is Blunted By Autophagic Inhibition In L6 Myotubes, Sean T. Stanelle, Patrick J. Ryan, Megan H. Lewis, Selina Uranga, Colleen L. O'Reilly, Jessica M. Cardin, James D. Fluckey

International Journal of Exercise Science: Conference Proceedings

Insulin is an anabolic hormone that acts on skeletal muscle cells to stimulate protein synthesis, an effect that is enhanced by the availability of amino acids. While autophagy within the cell provides an intracellular source of amino acids to support anabolism, little is known about how this pathway impacts the insulin-induced increase in anabolic capacity within skeletal muscle cells. PURPOSE: The purpose of this study was to determine the impact of autophagic inhibition in cultured L6 myotubes in conjunction with insulin stimulation in vitro. METHODS: Differentiated, cultured L6 myotubes were treated for 24 hours with or without insulin (100 nM) …

Autophagy Is Required For The Anabolic Response To Muscle Contraction, Patrick J. Ryan, Sean T. Stanelle, Megan H. Lewis, Selina Uranga, Colleen L. O'Reilly, J William Deaver, Jessica M. Cardin, James D. Fluckey

International Journal of Exercise Science: Conference Proceedings

Exercise is a key stimulus in regulating the behavior and metabolism of skeletal muscle, with exercise inducing muscular growth through activation of the anabolic mechanistic target of rapamycin kinase (mTOR). Separately, there is mounting evidence that exercise increases autophagy (one of the main routes by which intracellular proteins are degraded) and that the autophagic process may indeed be required for adaptations to exercise training. PURPOSE: To investigate the effects of autophagy inhibition on mTOR signaling and cellular anabolism after muscular contraction. METHODS: Cultured L6 myotubes were to exposed to electrical pulse stimulation using a stimulator set to deliver bipolar pulses …

Interview With Curtis J. Lewis, Zach S. Henderson Library Special Collections

Zach S. Henderson Library Special Collections Oral History Collection

Curtis J. Lewis was interviewed by Esther Mallard and Jay Fraser ca. 1988. The transcription was updated by Maggie H. Thomas, June 8, 2010.

Find this collection in the University Libraries' catalog!

Interview With Katrine Hawkins, Zach S. Henderson Library Special Collections

Zach S. Henderson Library Special Collections Oral History Collection

Katrine Hawkins interviewed by Esther Mallard, November 15, 1988. Find this collection in the University Libraries' catalog!

Information Needs And Seeking Behavior Of Distance And Regular Undergraduate University Students Of Khyber Pakhtunkhwa-Pakistan, Saeed Ullah Jan, Ihsan Ullah Khan

Library Philosophy and Practice (e-journal)

The main theme of this study is to investigate the information needs and seeking behavior of distance and regular Library and Information undergraduate students studying at different public sector universities of Khyber Pakhtunkhwa-Pakistan. Quantitative research method was used to carry out this research study. Adopted questionnaire was used as data collection tool. A total of 299 questionnaires were distributed among the respondents and 213 questionnaires were received. It was depicted that lectures and course books were the prime needed information of both groups for their academic qualifications. The prime needed information of both groups was found as “Information on course …

Use Of A Direct, Rapid Immunohistochemical Test For Diagnosis Of Rabies Virus In Bats, Charles E. Rupprecht, Lolita Van Pelt, April D. Davis, Richard B. Chipman, David L. Bergman

United States Department of Agriculture Wildlife Services: Staff Publications

Rabies, a zoonotic encephalitis due to transmission of a lyssavirus, such as rabies virus (RABV), has the highest case fatality of any infectious disease. A global program for the elimination of human rabies caused by dogs is proposed for realization by 2030. Sensitive, specific, and inexpensive diagnostic tests are necessary for enhanced surveillance to detect infection, inform public health and veterinary professionals during risk assessments of exposure, and support overall programmatic goals. Multiple laboratory techniques are used to confirm a suspect case of rabies. One method for the detection of lyssavirus antigens within the brain is the direct rapid immunohistochemical …

Female Assistant Superintendents: Using Personal Power To Overcome Self-Sabotage, Kristen Miller

Dissertations

Purpose: The purpose of this explanatory mixed-method study was to identify and describe self-sabotaging behaviors experienced by female assistant superintendents and to explore the impact these behaviors had on their career development. A secondary purpose of this study was to identify strategies employed by female assistant superintendents to overcome self-sabotaging behaviors.

Methodology: This explanatory sequential mixed-method study described the experiences of ten female assistant superintendents in California. The first phase of the study was quantitative and included an online survey designed to identify female assistant superintendents’ most prevalent self-sabotaging behaviors and the impact they had on their career …

An Exploration Of Trex1 Variants Of Uncertain Significance And Their Potential Impact On Autoimmunity In Systemic Lupus Erythematosus And Aicardi-Goutieres Syndrome, Jordan Peters

JSU Student Symposium 2022

TREX1 is a gene responsible for encoding a 3'-to-5' DNA exonuclease in human cells. Under normal conditions TREX1 removes bases from the free 3' end to enhance DNA damage and prevent DNA end reannealing and rapid repair. Mutations in TREX1, which result in the absence of the 3'-to-5' DNA exonuclease and the intracellular accumulation of unneeded DNA and RNA, have been shown to trigger immune dysfunction leading to diseases such as systemic lupus erythematosus (SLE) and Aicardi-Goutières Syndrome (AGS). The goal of this study is to determine the potential impact of a TREX1 missense mutation with uncertain clinical significance (VUS). …

Transgender Athletes And International Sports Policy, Joanna Harper

Law and Contemporary Problems

No abstract provided.

Deficiency Of Ataxia Telangiectasia-Mutated Kinase (Atm) Preserves Heart Function By Affecting Cardiac Remodeling In Response To Western-Type Diet (Wd) In Female Mice, Paulina Ramirez

Tennessee Posters at the Capitol [2022]

WD and deficiency of ATM protein independently associates with heart disease. Previous work demonstrated that WD in male ATM deficient mice induces accelerated body weight gain and heart dysfunction (Am J Physiol Heart Circ Physiol. 2021;320:H2324-H2338). Conversely, WD in female ATM deficient mice attenuates weight gain and preserves heart function (unpublished). Here, we investigated the mechanism by which ATM deficiency preserves heart function in female mice. Female wild-type (WT) and ATM heterozygous knockout (hKO) mice, aged 6 weeks, were fed with normal chow (NC) or WD for 14 weeks. Heart sections were stained with Masson’s trichrome to quantify fibrosis, TUNEL-stained …