Digital Commons Network^™

Anti-Dysplastic Effect Of Nettle Extract And Its Nano-Formulation On 7,12-Dimethylbenz[A]Anthracene Induced Hamster Buccal Pouch Carcinogenesis, Samah K. Ezzat, Amira M. Elsherbini

Mansoura Journal of Dentistry

Objective: Oral squamous cell carcinoma (OSCC) is the most common malignancy in the oral cavity with no definitive cure. In this study, chitosan loaded with nettle was used for the treatment of 7,12-Dimethylbenz[a] anthracene (DMBA)-induced oral squamous cell carcinoma compared to regular chemotherapy with 5-Fluorouracil (5-FU).Material and methods: Sixty pathogen-free male Syrian golden hamsters were used. The control group served as a negative control with no intervention, while the remaining animals received DMBA for 16 weeks then they were divided equally into 5 groups as follows; DMBA group received no treatment, while the 5-FU group was treated with 15mg/kg of …

Immunohistochemical Assessment Of Angiogenesis And Cell Proliferation In Aggressive Versus Non-Aggressive Central Giant Cell Granuloma., Mena F. Mesiha, Mahmoud F. El Sherbeny, Sherif Y. El Nagdy

Mansoura Journal of Dentistry

Objective: The current study investigated the expression of MCM3 and CD31 in aggressive versus non-aggressive central giant cell granuloma (CGCG ) Materials and methods: Thirty-three paraffin blocks for CGCG cases were collected from Oral Pathology Departments, Faculty of Dentistry, Mansoura, and Alexandria Universities. The Immunohistochemical staining for CD31 and MCM 3 was performed, assessed, and scored. Results: Mean age of the studied cases was 35.6±9.1 years. The lesions that occurred in the mandible represented the majority of cases [27 cases (81.8%)]. The study revealed an association between aggressiveness and the presence of large swelling, pain, cortical perforation, root resorption, and …

Stem Cell Marker As A Factor For The Different Biological Behavior Of Ameloblastoma And Odontogenic Keratocyst, Hanan M. Sayed Ahmed, Doaa A. Esmaeil, Sherif Y. Elnagdy

Mansoura Journal of Dentistry

Objective: To investigate the immunohistochemical expression of SOX2 among the studied cases of ameloblastoma and odontogenic keratocyst in order to show if it correlates to the biological behavior of those odontogenic lesions. Materials and Methods: The study was carried out on 30 histo-pathologically confirmed cases of (15 ameloblastomas and 15 odontogenic keratocysts). These blocks were collected from archived files of the Department of Oral Pathology, Faculty of Dentistry, Mansoura University. Sections were subjected to Immunohistochemical staining by SOX2 according to a standard protocol. Results: OKCs showed higher expression of SOX2 than Ab in all epithelial layers not confined to the …

Immunohistochemical Expression Of Ezh2 In Mucoepidermoid Carcinoma Of Salivary Glands, Amira A. Mansour, Mona M. Ibrahim, Ahmed E. Ahmed, Azza A. El Sissi

Mansoura Journal of Dentistry

Objective: To disclose the variation in EZH2 expression among the different grades of MEC and to analyze the marker's immunohistochemical (IHC) expression in relation to the clinicopathologic data of all cases. Material and methods: The present study was conducted on 15 blocks diagnosed as MEC including equal numbers of different grades. These blocks were selected from the archives of the Oncology Center, Mansoura University. The specimens embedded in the retrieved paraffin blocks were all fixed in 10% formalin. Sections of 4 microns’ thickness intervals were serially cut and prepared for the EZH2 staining. Chi-square, Monte Carlo, and Fisher Exact tests …

(R1981) Evaluating The Mhd Non-Newtonian Fluid Motion Past A Stretching Sheet Under The Influence Of Non-Uniform Thickness With Dufour And Soret Effects Implementing Chebyshev Spectral Method, M. M. Khader, Ram Prakash Sharma

Applications and Applied Mathematics: An International Journal (AAM)

A study is made on the development of hydromagnetic non-Newtonian Casson and Williamson boundary layer flow in an electrically conducting fluid in the presence of heat flux, mass flux, and the uniform magnetic field. The governing non-linear system of PDEs is transformed into a set of non-linear coupled ODEs and then treated numerically by using the Chebyshev spectral method. The velocity, temperature, and concentration fields of the steady boundary layer flow, which are generated by the stretched sheet with non-uniform thickness are discussed. The simultaneous effects of the external magnetic field, Soret and Dufour phenomena with reference have been explored. …

Earlier Snowmelt May Lead To Late Season Declines In Plant Productivity And Carbon Sequestration In Arctic Tundra Ecosystems, Donatella Zona, Peter M. Lafleur, Koen Hufkens, Barbara Bailey, Beniamino Gioli, George Burba, Jordan P. Goodrich, Anna K. Liljedahl, Eugénie S. Euskirchen, Jennifer D. Watts, Mary Farina, John S. Kimball, Martin Heimann, Mathias Göckede, Martijn Pallandt, Torben R. Christensen, Mikhail Mastepanov, Efrén López-Blanco, Marcin Jackowicz-Korczynski, Albertus J. Dolman, Luca Belelli Marchesini, Roisin Commane, Steven C. Wofsy, Charles E. Miller, David A. Lipson, Josh Hashemi, Kyle A. Arndt, Lars Kutzbach, David Holl, Julia Boike, Christian Wille, Torsten Sachs, Aram Kalhori, Xia Song

Daugherty Water for Food Global Institute: Faculty Publications

Arctic warming is affecting snow cover and soil hydrology, with consequences for carbon sequestration in tundra ecosystems. The scarcity of observations in the Arctic has limited our understanding of the impact of covarying environmental drivers on the carbon balance of tundra ecosystems. In this study, we address some of these uncertainties through a novel record of 119 site-years of summer data from eddy covariance towers representing dominant tundra vegetation types located on continuous permafrost in the Arctic. Here we found that earlier snowmelt was associated with more tundra net CO2 sequestration and higher gross primary productivity (GPP) only in June …

Predictions Of Indentation Stiffness Of Musculoskeletal Regions Using Ultrasound, Sean Doherty

ETD Archive

Tissue indentation response is an important metric for understanding how different musculoskeletal regions respond to loading and is a function of the tissue’s form. Modem imaging techniques provide information about the internal structures of human tissue. Ultrasound remains one of the most common imaging techniques performed, given its portability and low costs. Prior work and data collection on 100 patients involved the collection of ultrasound images at eight different locations across the musculoskeletal extremities. Given the tissue structure information that the medical imaging provided, it was hypothesized that the mechanical properties of the tissue could be predicted from this data. …

Rad51-Mediated Interhomolog Recombination During Budding Yeast Meiosis Is Promoted By The Meiotic Recombination Checkpoint And The Conserved Pif1 Helicase, Andrew Ziesel, Qixuan Weng, Jasvinder S. Ahuja, Abhishek Bhattacharya, Raunak Dutta, Evan Cheng, G. Valentin Borner Ph.D., Michael Lichten, Nancy M. Hollingsworth

Biological, Geological, and Environmental Faculty Publications

During meiosis, recombination between homologous chromosomes (homologs) generates crossovers that promote proper segregation at the first meiotic division. Recombination is initiated by Spo11-catalyzed DNA double strand breaks (DSBs). 5' end resection of the DSBs creates 3' single strand tails that two recombinases, Rad51 and Dmc1, bind to form presynaptic filaments that search for homology, mediate strand invasion and generate displacement loops (D-loops). D-loop processing then forms crossover and noncrossover recombinants. Meiotic recombination occurs in two temporally distinct phases. During Phase 1, Rad51 is inhibited and Dmc1 mediates the interhomolog recombination that promotes homolog synapsis. In Phase 2, Rad51 becomes active …

Mars 2100: A Microcosm Of Ecocentric Design, Andrea Hoe

Architecture Thesis Prep

In the dystopian future of 2100, Earth becomes a dying planet and humanity is forced to continue colonizing the solar system for survival. Mars develops as a colonized planet, exploited for its natural resources and potential. The hierarchical system of power seen constantly throughout Earth’s history was inescapable, and poor conditions turned to extremes. As billionaires take control of the space race, the capitalist system reflected in past company towns was implemented, making the moral questions and lines blurred.

When the system is eventually ruptured, ecosystemic living is proposed. Living is now centered around health and mindfulness, creating a system …

Paradigm Of The Post Natural: Critiquing Capitalist Ideals Through Environmental Degradation, Andrea De Haro, Charlotte Bascombe

Architecture Thesis Prep

There is an unspoken value in the destructiveness caused by design that is exposed through the exploitation of natural resources. This thesis seeks to exemplify the aesthetic value inherent within these newly defined environments. Nature is a source for human consumption, and as such it has developed into a commodity. By now it should be made evident that humanity’s presence is everywhere. There is no ecosystem left unturned by human manipulation Iin this respect, nature is dead. Deforested sites of oil fracking leave mile long toxic ponds highlighting the destructive pursuits that capitalism creates. Chernobyl showcases how human errors in …

Tectonic Thresholds: Reclaiming Space Through Geomorphological Design, Amreeta Verma

Architecture Thesis Prep

This research posits that a revitalization of indigenous earth architecture practices in a contemporary context can mitigate the immense waste and embodied carbon in the construction industry. Earth materials are the focus of this research because they are locally sourced, abundantly available, and can be reused or returned to the natural environment, when utilized in a circular consumption cycle. Designing for reuse reduces the impact of construction waste on the burgeoning issue of environmental degradation and resource depletion.

The structural viability and environmental enclosure capabilities of soil throughout its lifecycle will be tested in different forms derived geomorphically from the …

Tri-Ag News Academics Research Extension College Of Agriculture Sciences - Spring/Summer 2022, Prairie View A&M University

Prairie View Magazine Collection

No abstract provided.

Unmanned Aerial Vehicle Based Tree Canopy Characteristics Measurement For Precision Spray Applications, Md Sultan Mahmud, Long He, Paul Heinemann, Daeun Choi, Heping Zhu

Agricultural and Environmental Sciences Faculty Research

The critical components for applying the correct amount of agrochemicals are fruit tree characteristics such as canopy height, canopy volume, and canopy coverage. An unmanned aerial vehicle (UAV)-based tree canopy characteristics measurement system was developed using image processing approaches. The UAV captured images using a high-resolution red-green-blue (RGB) camera. A digital surface model (DSM) and a digital terrain model (DTM) were generated from the captured images. A tree canopy height map was generated from the subtraction of DSM and DTM. A total of 24 apple trees were randomly targeted to measure the canopy characteristics. Region of interest (ROI) was generated …

Tlr4/Nfκb-Mediated Anti-Inflammatory And Antioxidative Effect Of Hexanic And Ethanolic Extracts Of Curcuma Longa L. In Buffalo Mammary Epithelial Cells, Insha Amin, Shahzada Mudasir Rashid, Sheikh Shubeena, Ishraq Hussain

Faculty and Student Publications

Mastitis is the commonest disease of bovines imposing a great economic setback and a drastic public health concern worldwide. Antibiotic resistance is the preliminary con of excessive antibiotics use for disease treatment. Studies validate an alarming increase in the antibiotic resistance of both humans and animals. For ages, medicinal plants have been used to treat a number of ailments in humans and animals, including mastitis. Curcuma longa, or turmeric, is the prerequisite in Indian cooking and has been used in traditional medicine for its magical properties. The rhizome of C. longa possesses significant anti-inflammatory, antibacterial, antifungal, and antioxidative properties. In …

Immunogenetic Role Of Il17a Polymorphism In The Pathogenesis Of Recurrent Miscarriage, Shafat Ali, Sabhiya Majid, Md Niamat Ali, Mujeeb Zafar Banday

Faculty and Student Publications

Interleukin-17A (IL17A) is a proinflammatory cytokine and is assumed to play an important role in fetal rejection. In order to evaluate the potential role of IL17A polymorphism in the pathogenesis of recurrent miscarriage (RM), serum IL17A levels were estimated by ELISA. Single-nucleotide polymorphism was assessed by polymerase chain reaction-restriction fragment-length polymorphism (PCR-RFLP) using gene-specific primers and the EcoNI restriction enzyme. Serum IL17A levels were nonsignificantly (p > 0.5) low in RM patients compared with the control group. IL17A gene amplification by PCR yielded the undigested product of 815 bp, and its digestion with EcoNI enzyme produced 815, 529, 286, and 270 …

Antitrypanosomal, Antitopoisomerase-I, And Cytotoxic Biological Evaluation Of Some African Plants Belonging To Crassulaceae; Chemical Profiling Of Extract Using Uhplc/Qtof-Ms/Ms, Mostafa M. Hegazy, Wael M. Afifi, Ahmed M. Metwaly, Mohamed M. Radwan

Faculty and Student Publications

In our continuous study for some African plants as a source for antitrypanosomally and cytotoxic active drugs, nine different plants belonging to the Crassulaceae family have been selected for the present study. Sedum sieboldii leaves extract showed an antitrypanosomal activity against Trypanosoma brucei with an IC50 value of 8.5 µg/mL. In addition, they have cytotoxic activities against (HCT-116), (HEPG-2) and (MCF-7), with IC50 values of 28.18 ± 0.24, 22.05 ± 0.66, and 26.47 ± 0.85 µg/mL, respectively. Furthermore, the extract displayed inhibition against Topoisomerase-1 with an IC50 value of 1.31 µg/mL. It showed the highest phenolics and flavonoids content among …

Isolation And Characterization Of Flavonoid Naringenin And Evaluation Of Cytotoxic And Biological Efficacy Of Water Lilly (Nymphaea Mexicana Zucc.), Shajrath Din, Saima Hamid, Aadil Yaseen, Ali Mohd Yatoo

Faculty and Student Publications

Despite its limited exploration, Nymphaea mexicana Zucc. can be beneficial if pharmacology, isolation, and biological evaluation are given attention. It is an aquatic species that belongs to the family Nymphaeaceae. The thrust area of the work was the extraction, isolation, and biological evaluation of different extracts of the N. mexicana Zucc. plant. The primary goal of this research was to assess the antimicrobial, antioxidant, and anticancer activities of the extracts and to isolate the target naringenin compound. Comparative FT IR analysis of different extracts of this plant revealed the presence of functional groups of plant secondary metabolites, including polyphenols, flavonoids, …

Bioassay-Guided Fractionation With Antimalarial And Antimicrobial Activities Of Paeonia Officinalis, Mamdouh Nabil Samy, Basma Khalaf Mahmoud, Nourhan Hisham Shady, Usama Ramadan Abdelmohsen

Faculty and Student Publications

Bioassay-guided fractionation technique of roots of Paeonia officinalis led to isolation and structure elucidation of seven known compounds, including four monoterpene glycosides: lactiflorin (1), paeoniflorin (4), galloyl paeoniflorin (5), and (Z)-(1S,5R)-β-pinen-10-yl β-vicianoside (7); two phenolics: benzoic acid (2) and methyl gallate (3); and one sterol glycoside: β-sitosterol 3-O-β-D-glucopyranoside (6). The different fractions and the isolated compounds were evaluated for their antimicrobial and antimalarial activities. Fraction II and III showed antifungal activity against Candida neoformans with IC50 values of 28.11 and 74.37 µg/mL, respectively, compared with the standard fluconazole (IC50 = 4.68 µg/mL), and antibacterial potential against Pseudomonas aeruginosa (IC50 = …

Cannabidiol Loaded Topical Ophthalmic Nanoemulsion Lowers Intraocular Pressure In Normotensive Dutch-Belted Rabbits, Samir Senapati, Ahmed Adel Ali Youssef, Corinne Sweeney, Chuntian Cai

Faculty and Student Publications

Cannabidiol (CBD) is the major non-psychoactive and most widely studied of the cannabinoid constituents and has great therapeutic potential in a variety of diseases. However, contradictory reports in the literature with respect to CBD’s effect on intraocular pressure (IOP) have raised concerns and halted research exploring its use in ocular therapeutics. Therefore, the current investigation aimed to further evaluate CBD’s impact on the IOP in the rabbit model. CBD nanoemulsions, containing Carbopol® 940 NF as a mucoadhesive agent (CBD-NEC), were prepared using hot-homogenization followed by probe sonication. The stability of the formulations post-moist-heat sterilization, in terms of physical and chemical …

How Carbon Nanoparticles, Arbuscular Mycorrhiza, And Compost Mitigate Drought Stress In Maize Plant: A Growth And Biochemical Study, Emad A. Alsherif, Omar Almaghrabi, Ahmed M. Elazzazy, Mohamed Abdel-Mawgoud

Faculty and Student Publications

Drought negatively affects crop growth and development, so it is crucial to develop practical ways to reduce these consequences of water scarcity. The effect of the interactive potential of compost (Comp), mycorrhizal fungi (AMF), and carbon nanoparticles (CNPS) on plant growth, photosynthesis rate, primary metabolism, and secondary metabolism was studied as a novel approach to mitigating drought stress in maize plants. Drought stress significantly reduced maize growth and photosynthesis and altered metabolism. Here, the combined treatments Com-AMF or Com-AMF-CNPs mitigated drought-induced reductions in fresh and dry weights. The treatments with AMF or CNPS significantly increased photosynthesis (by 10%) in comparison …

Discovery Of A Lectin Domain That Regulates Enzyme Activity In Mouse N-Acetylglucosaminyltransferase-Iva (Mgat4a), Masamichi Nagae, Tetsuya Hirata, Hiroaki Tateno, Sushil K. Mishra

Faculty and Student Publications

N-Glycosylation is a common post-translational modification, and the number of GlcNAc branches in N-glycans impacts glycoprotein functions. N-Acetylglucosaminyltransferase-IVa (GnT-IVa, also designated as MGAT4A) forms a β1-4 GlcNAc branch on the α1-3 mannose arm in N-glycans. Downregulation or loss of GnT-IVa causes diabetic phenotypes by dysregulating glucose transporter-2 in pancreatic β-cells. Despite the physiological importance of GnT-IVa, its structure and catalytic mechanism are poorly understood. Here, we identify the lectin domain in mouse GnT-IVa’s C-terminal region. The crystal structure of the lectin domain shows structural similarity to a bacterial GlcNAc-binding lectin. Comprehensive glycan binding assay using 157 glycans and solution NMR …

Milk-Compositional Study Of Metabolites And Pathogens In The Milk Of Bovine Animals Affected With Subclinical Mastitis, Aarif Ali, Manzoor Ur Rahman Mir, Showkat Ahmad Ganie, Saima Mushtaq

Faculty and Student Publications

Bovine milk is an important food component in the human diet due to its nutrient-rich metabolites. However, bovine subclinical mastitis alters the composition and quality of milk. In present study, California mastitis testing, somatic cell count, pH, and electrical conductivity were used as confirmatory tests to detect subclinical mastitis. The primary goal was to study metabolome and identify major pathogens in cows with subclinical mastitis. In this study, 29 metabolites were detected in milk using gas chromatography–mass spectrometry. Volatile acidic compounds, such as hexanoic acid, hexadecanoic acid, lauric acid, octanoic acid, n-decanoic acid, tricosanoic acid, tetradecanoic acid, and hypogeic acid …

Validated Determination Of Nrg1 Ig-Like Domain Structure By Mass Spectrometry Coupled With Computational Modeling, Niloofar Abolhasani Khaje, Alexander Eletsky, Sarah E. Biehn, Charles K. Mobley

Faculty and Student Publications

High resolution hydroxyl radical protein footprinting (HR-HRPF) is a mass spectrometry-based method that measures the solvent exposure of multiple amino acids in a single experiment, offering constraints for experimentally informed computational modeling. HR-HRPF-based modeling has previously been used to accurately model the structure of proteins of known structure, but the technique has never been used to determine the structure of a protein of unknown structure. Here, we present the use of HR-HRPF-based modeling to determine the structure of the Ig-like domain of NRG1, a protein with no close homolog of known structure. Independent determination of the protein structure by both …

Utility Of Fatty Acid Profile And In Vitro Immune Cell Activation For Chemical And Biological Standardization Of Arthrospira/Limnospira, Jungmoo Huh, Jin Zhang, Radka Hauerová, Joseph Lee

Faculty and Student Publications

Commercially cultivated Limnospira (species formerly classified to genus Arthrospira) is a popular food/supplement consumed by millions of people worldwide for health benefits. The objective of the current research was to advance the standardization technology for Limnospira. Quantitative methods were established to detect fatty acids as potential chemical markers and immune-enhancing activity. Analysis of 20 different batches of biomass obtained from one commercial grower demonstrated that there was a statistically significant relationship between the sum of two fatty acids (linoleic and γ-linolenic) and Toll-like receptor (TLR)2/TLR1-dependent activation (R2 = 0.48, p = 0.0007). Investigation of 12 biomass samples sourced from growers …

A Distinct Mechanism Of C-Type Inactivation In The Kv-Like Kcsa Mutant E71v, Ahmed Rohaim, Bram J. A. Vermeulen, Jing Li, Felix Kümmerer

Faculty and Student Publications

C-type inactivation is of great physiological importance in voltage-activated K+ channels (Kv), but its structural basis remains unresolved. Knowledge about C-type inactivation has been largely deduced from the bacterial K+ channel KcsA, whose selectivity filter constricts under inactivating conditions. However, the filter is highly sensitive to its molecular environment, which is different in Kv channels than in KcsA. In particular, a glutamic acid residue at position 71 along the pore helix in KcsA is substituted by a valine conserved in most Kv channels, suggesting that this side chain is a molecular determinant of function. Here, a combination of X-ray crystallography, …

Msw Capstone, Angela Marple

Social Work Masters Capstone Projects

abstract not available

(R1885) Analytical And Numerical Solutions Of A Fractional-Order Mathematical Model Of Tumor Growth For Variable Killing Rate, N. Singha, C. Nahak

Applications and Applied Mathematics: An International Journal (AAM)

This work intends to analyze the dynamics of the most aggressive form of brain tumor, glioblastomas, by following a fractional calculus approach. In describing memory preserving models, the non-local fractional derivatives not only deliver enhanced results but also acknowledge new avenues to be further explored. We suggest a mathematical model of fractional-order Burgess equation for new research perspectives of gliomas, which shall be interesting for biomedical and mathematical researchers. We replace the classical derivative with a non-integer derivative and attempt to retrieve the classical solution as a particular case. The prime motive is to acquire both analytical and numerical solutions …

(R1980) Effect Of Climate Change On Brain Tumor, Pardeep Kumar, Sarita Jha, Rajiv Aggarwal, Govind Kumar Jha

Applications and Applied Mathematics: An International Journal (AAM)

In this paper, we introduce a new dynamical model addressing the variation in climate condition due the presence of microorganisms. We also introduce a new dynamical model of cancer growth which includes three interactive cell populations with drug free environment, namely tumor cells, healthy host cells, and immune effector cells. In this, we considered the super growth of tumor cells. For the choice of certain parameters, both of the systems exhibit chaotic behavior. The aim of this work is to design the controller to control the chaos and to provide sufficient conditions which achieve synchronization of two non-identical systems, which …

Understanding And Improving The Activity Of Oxygen Evolution Reaction Catalysts In Alkaline And Chloride-Containing Electrolytes, Prajwal Adiga

Link Foundation Energy Fellowship Reports

Hydrogen is a feedstock in many chemical processes and can also be used as an energy carrier to store, move, and deliver energy produced from other sources. Recently, hydrogen is of interest for storing renewable electricity and helping address the intermittent nature of electricity generation from renewable sources.1 Water electrolysis plays a pivotal role in achieving a green hydrogen economy by sourcing electricity from intermittent renewable sources. However, the efficiency of the electrolysis process is limited by the sluggish oxygen evolution reaction (OER) kinetics,2-5 whereas the kinetics of the hydrogen evolution reaction (HER) are more facile. My work focused on …

Novel Techniques For Quantifying Secondhand Smoke Diffusion Into Children's Bedroom, Sunil Ramchandani

Computational and Data Sciences (PhD) Dissertations

The impact of secondhand smoking to health of general population, and specifically children, is a well-known phenomenon that researchers have studied for years. In this dissertation, I extend work done within a secondhand smoke intervention to understand and quantity the impact of intervention on flow of smoke air particle concentration from the main room where smoking generates air particle contamination to a room where a child living in the home sleeps. The paper also explores potential modelling techniques to proactively identify and the impact of the smoke air particles with the intent to discourage adults from smoking in the home …