Digital Commons Network^™

A Dual-Function Gene In Zea Mays That Encodes Two Β-Amylase Proteins With Different Cellular Locations And Functions, Kenan Ozcan

Masters Theses, 2020-current

A noncanonical β-amylase7 (BAM7) gene found in some basal angiosperms and some monocots, including corn, appears to encode two functionally distinct proteins. Phylogenetic evidence suggests that BAM7 originated from a fusion event that combined a BZR1-like DNA-binding domain to the 5’ end of BAM2 about 400 million years ago (mya). In Arabidopsis thaliana (At), AtBAM7 is a catalytically-inactive transcription factor found in the nucleus and AtBAM2 is a catalytically-active, tetrameric starch hydrolase found in the chloroplast. These proteins are encoded on different chromosomes. The corn (Zea mays, Zm) genome does not contain a BAM2 gene, however …

Office Of Research, Economic Engagement And Outreach Update, July 13, 2022, Office Of Research, Economic Engagement And Outreach

Office of Research, Economic Engagement and Outreach

No abstract provided.

The Lived Experience Of Postpartum Anxiety During Covid-19: A Hermeneutic Phenomenological Study, Walker Ladd Phd, Jenny De Decker

The Qualitative Report

The experience of pregnancy and postpartum anxiety disorders results in adverse birth outcomes and the disrupted development of infants and children. Since the COVID-19 Pandemic, the Centers for Disease Control and Prevention (CDC) has designated pregnant and postpartum women as more vulnerable to COVID-19 (CDC, 2021), and perinatal mood and anxiety disorders rates have increased. However, research regarding the lived experience of women with postpartum anxiety (PPA) during a global pandemic remains lacking. Using van Manen’s hermeneutic phenomenological research method, we interviewed eight women self-identifying as having had PPA during the COVID-19 pandemic. Analysis revealed five themes describing the lived …

The George-Anne Daily, Georgia Southern University

George-Anne Media Group: Newsletters & Magazines

No abstract provided.

Optimised Adjoint Sensitivity Analysis Using Adjoint Guided Mesh Adaptivity Applied To Neutron Detector Response Calculations, Andrew G. Buchan, Dan Gabriel Cacuci, Steven Dargaville, Christopher C. Pain

Faculty Publications

This article presents a new approach for the efficient calculation of sensitivities in radiation dose estimates, subject to imprecisely known nuclear material cross-section data. The method is a combined application of adjoint-based models to perform, simultaneously, both the sensitivity calculation together with optimal adaptive mesh refinement. Adjoint-based sensitivity methods are known for their efficiency since they enable sensitivities of all parameters to be formed through only two solutions to the problem. However, the efficient solutions can also be obtained by their computation on optimal meshes, here guided by goal-based adjoint approaches. It is shown that both mesh adaptivity and sensitivity …

Too Much Of A Good Thing? A Governing Knowledge Commons Review Of Abundance In Context, Brett M. Frischmann, Michael J. Madison, Madelyn R. Sanfilippo, Katherine J. Strandburg

Faculty Publications

The economics of abundance, along with the sociology of abundance, the law of abundance, and so forth, should be re-framed, linked, and situated in a common context for empirical rather than conceptual research. Abundance may seem to be a new, big thing, between anxiety over information overload, Big Data, and related technological disruptions. But scholars know that abundance is an ancient phenomenon, which only seemed to disappear as twentieth century social science focused on scarcity instead. Restoring the study of abundance, and figuring out how to solve the problems that abundance might create, means shedding disciplinary blinders and going back …

Gut Bacterial Isoamylamine Promotes Age-Related Cognitive Dysfunction By Promoting Microglial Cell Death, Yun Teng, Jingyao Mu, Fangyi Xu, Xiangcheng Zhang, Mukesh K Sriwastva, Qiaohong M Liu, Xiaohong Li, Chao Lei, Kumaran Sundaram, Xin Hu, Lifeng Zhang, Juw Won Park, Jae Yeon Hwang, Eric C Rouchka, Xiang Zhang, Jun Yan, Michael L Merchant, Huang-Ge Zhang

Faculty, Staff and Student Publications

The intestinal microbiome releases a plethora of small molecules. Here, we show that the Ruminococcaceae metabolite isoamylamine (IAA) is enriched in aged mice and elderly people, whereas Ruminococcaceae phages, belonging to the Myoviridae family, are reduced. Young mice orally administered IAA show cognitive decline, whereas Myoviridae phage administration reduces IAA levels. Mechanistically, IAA promotes apoptosis of microglial cells by recruiting the transcriptional regulator p53 to the S100A8 promoter region. Specifically, IAA recognizes and binds the S100A8 promoter region to facilitate the unwinding of its self-complementary hairpin structure, thereby subsequently enabling p53 to access the S100A8 promoter and enhance S100A8 expression. …

The Intrinsically Disordered Protein Tgist From Toxoplasma Gondii Inhibits Stat1 Signaling By Blocking Cofactor Recruitment, Zhou Huang, Hejun Liu, Jay Nix, Rui Xu, Catherine R Knoverek, Gregory R Bowman, Gaya K Amarasinghe, L David Sibley

2020-Current year OA Pubs

Signal transducer and activator of transcription (STAT) proteins communicate from cell-surface receptors to drive transcription of immune response genes. The parasite Toxoplasma gondii blocks STAT1-mediated gene expression by secreting the intrinsically disordered protein TgIST that traffics to the host nucleus, binds phosphorylated STAT1 dimers, and occupies nascent transcription sites that unexpectedly remain silenced. Here we define a core region within internal repeats of TgIST that is necessary and sufficient to block STAT1-mediated gene expression. Cellular, biochemical, mutational, and structural data demonstrate that the repeat region of TgIST adopts a helical conformation upon binding to STAT1 dimers. The binding interface is …

Percent Grade Scale Amplifies Racial Or Ethnic Inequities In Introductory Physics, Cassandra A. Paul, David J. Webb

Faculty Research, Scholarly, and Creative Activity

In previous work we analyzed databases for 95 classes to show that the percent grade scale was correlated with a much higher student fail rate than the 4.0 grade scale. This paper builds on this work and investigates equity gaps occurring under both scales. By employing a “course deficit model” we attribute the responsibility for closing the gaps to those who are responsible for the policies that guide the course. When comparing course grades in classes graded using the percent scale with those in courses graded using the 4.0 scale, we find that students identifying as belonging to racial or …

Abundance, Site-Fidelity, And Association Patterns Of Coastal Bottlenose Dolphins (Tursiops Truncatus) Off Southeast Florida, Graysen D. Boehning

All HCAS Student Capstones, Theses, and Dissertations

The coastal bottlenose dolphin is well studied throughout its natural range, however, most of the study areas comprised wide, well-protected habitats such as bays and estuaries, and not narrow coastal sandbanks. This study identifies a residential group of coastal bottlenose dolphins utilizing the narrow sandbanks within the Northwestern Atlantic waters off the coast of Palm Beach County, Florida, USA. From 2014-2020, 313 boat surveys were conducted, and 585 individual dolphins were identified using photo-ID. Twenty-four animals were determined to be full-time and 66 animals were determined to be part-time residents. Full-time and part-time residents associated in three social tribes, with …

Consequences Of Gulf Of Mexico Estuarine Tropical Cyclone Disturbance Regimes For Fish Assemblage Dynamics Conditionally Depend On Life-History Traits And Niche Characteristics, Stephen Edward Potts

LSU Doctoral Dissertations

Tropical cyclones (TC) are intense, localized disturbances that can potentially cause extensive damage to ecosystems. This dissertation is motivated by a need for interdisciplinary study of TC-ecosystem dynamics with applications to ecology, geography, and analytical methodology. I explore concept linkages between TC dynamics and ecosystems to characterize TC-ecosystem relationships as ecological disturbance regimes. This dissertation tests hypotheses and relationships of TC effects on estuarine fish assemblages in the north-western Gulf of Mexico. I explore concepts in TC-ecosystem relationships to link biotic dynamics with physical disturbance.

In Chapter 2, I compared spatial performance of generalized linear models (GLMs) and generalized additive …

What Nations Owe Each Other Before The Next Pandemic, Lawrence O. Gostin, Kevin A. Klock, Sam F. Halabi, Katie Gottschalk, Katherine Ginsbach, Kashish Aneja

Georgetown Law Faculty Publications and Other Works

On December 1, 2021, the World Health Assembly adopted a resolution establishing an Intergovernmental Negotiating Body (INB) to determine the content and form of a new pandemic agreement. A portion of the public has advocated for a non-binding agreement while others stress that nationalism should be prevented, with steps taken to monitor and enforce national compliance. The INB has needed to grapple with how the principle of national sovereignty, and the accompanying principle of non-interference, will be addressed with respect to the agreement’s content and form, including obligations to share data, resources, and personnel, and to relinquish control over certain …

The Testis-Specific E3 Ubiquitin Ligase Rnf133 Is Required For Fecundity In Mice, Kaori Nozawa, Yoshitaka Fujihara, Darius J Devlin, Ricardo E Deras, Katarzyna Kent, Irina V Larina, Kohei Umezu, Zhifeng Yu, Courtney M Sutton, Qiuji Ye, Laura K Dean, Chihiro Emori, Masahito Ikawa, Thomas X Garcia, Martin M Matzuk

Faculty, Staff and Students Publications

BACKGROUND: Ubiquitination is a post-translational modification required for a number of physiological functions regulating protein homeostasis, such as protein degradation. The endoplasmic reticulum (ER) quality control system recognizes and degrades proteins no longer needed in the ER through the ubiquitin-proteasome pathway. E2 and E3 enzymes containing a transmembrane domain have been shown to function in ER quality control. The ER transmembrane protein UBE2J1 is a E2 ubiquitin-conjugating enzyme reported to be essential for spermiogenesis at the elongating spermatid stage. Spermatids from Ube2j1 KO male mice are believed to have defects in the dislocation step of ER quality control. However, associated …

Great Lakes, Great Books, Lynette Marten Suckow

Michigan Reading Journal

No abstract provided.

Engaging Boys In Literacy And Elementary School, Rebecca R. Norman

Michigan Reading Journal

No abstract provided.

Minutes 7-12-2022 | Safety Committee, Natalia Esparragoza

Safety Committee Minutes

No abstract provided.

Upwelling-Level Acidification And Ph/Pco2 Variability Moderate Effects Of Ocean Acidification On Brain Gene Expression In The Temperate Surfperch, Embiotoca Jacksoni, Jason A. Toy, Kristy J. Kroeker, Cheryl A. Logan, Yuichiro Takeshita, Gary C. Longo, Giacomo Bernardi

Marine Science Faculty Publications and Presentations

Acidification-induced changes in neurological function have been documented in several tropical marine fishes. Here, we investigate whether similar patterns of neurological impacts are observed in a temperate Pacific fish that naturally experiences regular and often large shifts in environmental pH/pCO2. In two laboratory experiments, we tested the effect of acidification, as well as pH/pCO2 variability, on gene expression in the brain tissue of a common temperate kelp forest/estuarine fish, Embiotoca jacksoni. Experiment 1 employed static pH treatments (target pH = 7.85/7.30), while Experiment 2 incorporated two variable treatments that oscillated around corresponding static treatments with …

Ci Asteroid Regolith As An In Situ Plant Growth Medium For Space Crop Production, Steven J. Russell, Sherry Fieber-Beyer, Kathryn A. Yurkonis

Biology Faculty Publications

Human expansion into the solar system is currently at the forefront of space research. For our astronauts to survive, they will need to be fed a healthy and nutritious diet on a consistent basis. Right now, our current method of feeding astronauts consists of resupplied prepackaged food from Earth, which is unsustainable for long-term missions. Using planetary resources via in situ resource utilization to grow crops is the next step toward sustainability in space. Asteroids are an abundant space resource and should not be overlooked when considering crewed missions. In particular, the primordial CI carbonaceous asteroids are of interest because …

A Pilot Study On The Nanoscale Properties Of Bone Tissue Near Lacunae In Fracturing Women, Wen Qian, Roman Schmidt, Joseph A. Turner, Sue P. Bare, Joan M. Lappe, Robert R. Recker, Mohammed P. Akhter

Department of Mechanical and Materials Engineering: Faculty Publications

The goal of this study is to investigate the causes of osteoporosis-related skeletal fragility in postmenopausal women. We hypothesize that bone fragility in these individuals is largely due to mineral, and/or intrinsic material properties in the osteocyte lacunar/peri-lacunar regions of bone tissue. Innovative measurements with nanoscale resolution, including scanning electron microscope (SEM), an atomic force microscope that is integrated with infrared spectroscopy (AFM-IR), and nanoindentation, were used to characterize osteocyte lacunar and peri-lacunar properties in bone biopsies from fracturing (Cases) and matched (Age, BMD), non-fracturing (Controls) postmenopausal healthy women. In the peri-lacunar space, the nanoindentation results show that the modulus …

Solid State Touchchromic Device, Manoj Kumar Ram, D. Yogi Goswami, Lee K. Stefanakos

USF Patents

Disclosed herein is a solid touchchromic device. The solid touchchromic device may include a conducting polymer or a conducting polymer composite film, a conducting plate, and a solid layer of a polymer-based electrolyte, the conducting plate being at least partially coated by the conducting polymer or the conducting polymer composite film. The solid touchchromic device may further include an oxidant, a salt, an acid, or a metal. Also included are methods of producing a solid touchchromic device and articles including a solid touchchromic device.

Hip Torque Is A Mechanistic Link Between Sprint Acceleration And Maximum Velocity Performance: A Theoretical Perspective, Kenneth P. Clark, Laurence J. Ryan

Kinesiology Faculty Publications

Sprinting performance is critical for a variety of sports and competitive activities. Prior research has demonstrated correlations between the limits of initial acceleration and maximum velocity for athletes of different sprinting abilities. Our perspective is that hip torque is a mechanistic link between these performance limits. A theoretical framework is presented here that provides estimates of sprint acceleration capability based on thigh angular acceleration and hip torque during the swing phase while running at maximum velocity. Performance limits were calculated using basic anthropometric values (body mass and leg length) and maximum velocity kinematic values (contact time, thigh range of motion, …

Increased Cytokine Gene Expression And Cognition Risk Associated With Androgen Deprivation Therapy, Shiv Verma, Prem Prakash Kushwaha, Eswar Shankar, Lee E. Ponsky, Sanjay Gupta

Faculty Scholarship

Background: Androgen deprivation therapy (ADT) is a standard treatment modality for locally advanced, high-risk, and metastatic hormone-sensitive prostate cancer. Long-term ADT treatment likely develops side-effects that include changes in cognition or onset of dementia. However, the molecular understanding of this effect remains elusive. We attempt to establish a link between ADT and changes in cognitive function using patient databases and bioinformatics analyses. Methods: Gene expression profiling was performed using RNA sequencing data from Alzheimer patient cohort and compared with the data from advanced-stage prostate cancer patients receiving neoadjuvant antiandrogen therapy. Differentially expressed genes (DEGs) were analyzed using the Ingenuity knowledge …

Synthesis And Evaluation Of Anti-Hiv Activity Of Mono- And Di-Substituted Phosphonamidate Conjugates Of Tenofovir, Aaminat Qureshi, Louise A. Ouattara, Naglaa Salem El-Sayed, Amita Verma, Gustavo F. Doncel, Muhammad Iqbal Choudhary, Hina Siddiqui, Keykavous Parang

Pharmacy Faculty Articles and Research

The activity of nucleoside and nucleotide analogs as antiviral agents requires phosphorylation by endogenous enzymes. Phosphate-substituted analogs have low bioavailability due to the presence of ionizable negatively-charged groups. To circumvent these limitations, several prodrug approaches have been proposed. Herein, we hypothesized that the conjugation or combination of the lipophilic amide bond with nucleotide-based tenofovir (TFV) (1) could improve the anti-HIV activity. During the current study, the hydroxyl group of phosphonates in TFV was conjugated with the amino group of L-alanine, L-leucine, L-valine, and glycine amino acids and other long fatty ester hydrocarbon chains to synthesize 43 derivatives. Several …

Tom Prieur, Oral History Interview, 2022, Matt Jones

Oral Histories

Tom Prieur was active with the Arm of Honor Fratrernity from 1960 until 1963. Coming to EMU from Alpena, Michigan, Prieur is said to have brought several athletes into the fraternity, allowing the fraternity to achieve its athletic dominance on the EMU intramural fields. Quarterback for the EMU football team, Prieur, known as “Puss” to his Arm brothers, describes his introduction to athletics as a child, the occupations of his parents, and being recruited for the FBI following college graduation. Prieur speaks of living in the frat house, hazing rituals, and off-campus hang outs of the fraternity brothers. Prieur also …

Electrochemical Reduction Of Hexavalent Chromium On Glassy Carbon Electrodes In Acidic Buffers, Callie M. Stern

LSU Doctoral Dissertations

As fresh water systems become endangered due the expansion of growth and human civilization, it is important to find effective water purification strategies. A common water contaminant that is mostly generated through industrial processes is hexavalent chromium. The two stable forms of chromium found in aqueous systems are hexavalent chromium, Cr(VI), and trivalent chromium, Cr(III). Considered an essential nutrient, Cr(III) is non-toxic to humans. Cr(VI) on the other hand, is toxic at low concentrations and can be ingested through drinking contaminated water. There is no standard limit to the amount of Cr(VI) that can be in drinking water and most …

Developmental Competence Of Oocytes Derived From Seasonally Anovulatory Mares Treated With Estradiol And Sulpiride, Michelle O. Vetter

LSU Master's Theses

Over the course of two years (2021-2022), two experiments were conducted during the nonbreeding seasons to evaluate the in vitro developmental potential of oocytes derived from seasonally anovulatory mares treated with ECP-sulpiride. The objective of experiments 1 and 2 were to compare pooled recovery and maturation rates of oocytes from mares treated with estradiol cypionate (ECP) and sulpiride with oocytes collected from naturally transitional mares (controls). Cleavage rates of oocytes subjected to intracytoplasmic sperm injection (ICSI) were compared only in experiment 2. In experiments 1 and 2, a total of 36 light horse and pony-cross anovulatory mares were used. Treatment …

Baseline Differences In Driving Frequency As A Predictor Of Cognitive Decline, Luke R. Miller

LSU Master's Theses

Driving is a complex task heavily dependent on cognitive functions which can decline with age including executive functions and processing speed. Although driving cessation as a predictor of cognitive changes has been studied, driving frequency is understudied in the literature. Thus, the objective of the current study is to evaluate the predictive utility of driving frequency at baseline toward objective cognitive decline beyond other factors associated with cognitive decline (e.g., depression, general functional mobility). The sample included a subset of 1,426 older adults (M age = 77.6, SD = 7.1) from the Rush University Memory and Aging Project. Participants completed …

My Flyer Family: Pinning Together Four Generations, Elizabeth Buergler

Materials from the University of Dayton Magazine Website

In 2000, Elizabeth Buergler ’05 visited her grandfather's alma mater on her college tour just to humor him. She ended up falling in love with the University of Dayton, as he had decades earlier. This essay is part of the My Flyer Family series.

Synthesis Of Boron-Doped Zinc Oxide Nanosheets By Using Phyllanthus Emblica Leaf Extract: A Sustainable Environmental Applications, Awais Khalid, Pervaiz Ahmad, Saleh Muhammad, Abdulhameed Khan, Mayeen Uddin Khandaker, Md Mottahir Alam, Mohd Asim, Israf Ud Din, Jibran Iqbal, Ibad Ur Rehman, Zohaib Razzaq, Sivakumar Pandian, Rohit Sharma, Talha Bin Emran, M. I. Sayyed, Saad Aldawood, Abdelmoneim Sulieman

All Works

The use of Phyllanthus emblica (gooseberry) leaf extract to synthesize Boron-doped zinc oxide nanosheets (B-doped ZnO-NSs) is deliberated in this article. Scanning electron microscopy (SEM) shows a network of synthesized nanosheets randomly aligned side by side in a B-doped ZnO (15 wt% B) sample. The thickness of B-doped ZnO-NSs is in the range of 20–80 nm. B-doped ZnO-NSs were tested against both gram-positive and gram-negative bacterial strains including Staphylococcus aureus, Pseudomonas aeruginosa, Klebsiella pneumonia, and Escherichia coli. Against gram-negative bacterium (K. pneumonia and E. coli), B-doped ZnO displays enhanced antibacterial activity with 26 and 24 mm of inhibition zone, respectively. …

The Dunning-Kruger Effect On Free Throw Shooting, Abigail Schescke, Nicolas Veldhorst, Makayla De Young

Student Work

This study examines the Dunning-Kruger effect in regards to physical activity. Seventy-four participants with various levels of basketball experience were recruited from an undergraduate university. Participants were assigned to make predictions regarding the number of free throw shots they would make out of ten before or after warming up. Out of factors which affected participant predictions (gender, previous experience, warm up group), gender was found to have a significant relationship with predictive error score. Additionally, underconfidence from top-performing participants and overconfidence from bottom-performing participants indicates the presence of the Dunning-Kruger effect in physical tasks.