Growth Outcomes Of Pseudomonas Aeruginosa Inhibitor Of Vertebrate Lysozyme Knockouts In Conditions Mimicking The Cystic Fibrosis Lung Environment, Amani Gaddy

Master of Science in Chemical Sciences Theses

Pseudomonas aeruginosa (PA) is a Gram-negative bacterium, often found in cystic fibrosis (CF) patients and can lead to the decline of lung functioning and premature death in 80% of infected patients when microcolonies form within the mucin of the lung. Due to its major capacity for antibiotic resistance, an alternative strategy towards defending against the bacterial invasion of PA is by the antibacterial activity of our own innate immune system with use of elements such as lysozyme. Pseudomonas aeruginosa inhibitor of vertebrate lysozyme class 1 (Ivyp1) is a periplasmic protein produced by gram-negative bacteria that inhibits the enzymatic activity of …

In Vitro And In Silico Analysis Of The Inhibitory Activity Of Egcg-Stearate Against Herpes Simplex Virus-2, James D. Stamos, Lee H. Lee, Calvin Taylor, Tony Elias, Sandra D. Adams

Department of Biology Faculty Scholarship and Creative Works

About half a billion people worldwide are infected with herpes simplex virus-2 (HSV-2). Prolonged treatment with acyclovir (ACV) and its analogs leads to the development of resistant strains. The aim of this study was to investigate the antiviral potential of epigallocatechin gallate (EGCG) from Camellia sinensis and a stable analog EGCG-stearate (EGCG-S) against HSV-2 in cultured Vero cells. Cell viability and cell proliferation assays were used to determine the non-cytotoxic concentrations on cultured Vero cells. HSV-2 with a green fluorescent protein (GFP) fusion protein of VP26 virions were treated with non-cytotoxic concentrations of EGCG and EGCG-S. The effects on infectivity …

An Exploration Of The Axiom “Show, Don’T Tell” In Fiction Writing, Tina Matras

Masters Theses

This thesis is about one person’s journey from the stringent, fact-based world of technical writing to a creative place known as fiction writing. The Artist Statement explores the author’s journey into the world of creative writing and choice of genre. The critical paper is a sample of the process the author undertook to learn how to “show” a story. Instructional technical writers provide readers with a set of instructions or steps from information that already exists. Nothing is made up, and any visual provided is accompanied by pictures. The reader doesn’t have to imagine anything. Fiction writers must help a …

Resilience In Black Mental Health Counselors, Ericka Cables

Dissertations

Though resilience is a commonly studied variable in different disciplines, there is relatively little research on occupational settings. Researchers take various perspectives in defining resilience as a trait, process, and an outcome. Regardless of how it is defined, it is a vital protective factor for Black mental health counselors who may be significantly impacted by similar experiences, such as racism and racial discrimination, as the Black clients they serve. This dissertation study consisted of two studies utilizing the same data set. In the first study, psychometric properties of a new quantitative instrument, the Race-Based Resilience Scale (RBRS), was constructed to …

Research Progress Of Protein-Based Plant Polyphenol Nano-Delivery Carriers, Chen Yang-Ling, Jiao Ye, Cui Bo, Fang Fang, Yang Jing-Jie, Cheng Yun-Hui

Food and Machinery

This review summarized the research progress on the preparation methods of nano-delivery systems, the types of proteins used to construct plant polyphenol nano-delivery carriers, and the modification of nano-delivery carriers by cross-linking agents domestically and internationally. The development of protein-based plant polyphenol nano-delivery carriers in the food field was also prospected.

Inhibitory Effect Of Sea Cucumber Coelomic Fluid Extracts On White Shrimp Autolysis, Nie Bin, Li Ao-Ting, Cui Xiao-Fan, Du Yi-Nan, Yan Jia-Nan, Wu Hai-Tao

Food and Machinery

Objective:In this paper, the inhibition of protein degradation in autolysis of white shrimp (Penaeus vannamei) surimi by extracts from the coelomic fluid of sea cucumber (Stichopus japonicus), a by-product of sea cucumber processing, was studied.Methods:The protein degradation in autolysis of white shrimp was studied by SDS-PAGE assay. Furthermore, the effect of sea cucumber coelomic fluid and its extracts on white shrimp protein degradation was determined by SDS-PAGE and TCA-soluble oligopeptide assay.Results:The optimal conditions of white shrimp autolysis were incubation at pH 3.0, 50 ℃ for 2 h. The bond intensities of myosin heavy chain around …

Effect Of Sulfate Modificate On The Structure And Antioxidant Activity Of Jujube Polysaccharide, Gong Xiao-Pei, Zhang Jian, Guo Xiao-Bin, Mao Xiao-Ying, Zhang Lian-Fu

Food and Machinery

Objective:This study aimed to explore the relationship between the structure and antioxidant activity of purified red date polysaccharide ZJP-1 and sulfated red date polysaccharide S-ZJP-1.Methods:Using remnant jujube as raw material, jujube polysaccharide (ZJP-1) was purified by hot water extraction, and then separated by DEAE-52 ion exchange chromatography and Sephadex-200 dextran gel column chromatography. Thereafter, red jujube polysaccharide sulfated derivative (S-ZJP-1) was obtain through chlorosulfonic acid-pyridine sulfated modification.Results:Both ZJP-1 and S-ZJP-1 are composed of three monosaccharides: glucose, arabinose, and galactose, but the ratios are different. The two polysaccharides had characteristic absorption peaks of polysaccharides, and S-ZJP-1 …

Analysis Of Kombucha Microflora And The Isolation And Identification Of Dominant Bacteria, Xu Su-Yun, Li Jia-Jia, Fang Wei, Wang Yan-Ping, Geng Wei-Tao

Food and Machinery

Objective:To understand bacterial composition and obtain isolated fermentative strains found in kombucha.Methods:The bacterial composition and dominant strains were analyzed using 16S rDNA high-throughput sequencing technology and traditional microbial culture method.Results:High-throughput sequencing showed that the bacterial community is richer and more diverse than the fungi community. Komagataeibacter,Acetobacter and Lactobacillus were the dominant bacterial strains, while Dekkera and Zygosacchaormyces were the dominant fungal strains. At the same time, 7 strains of acetic acid bacteria, 3 strains of yeast and 1 strain of lactic acid bacteria were isolated by traditional plate culture method, including 1 strain of Komagataeibacter …

Differential Analysis Of Volatile Substances In Fresh Gastrodia Elata From Different Varieties And Origin, Sun Hai-Yan, Hao Dan-Qing, Li Xin-Sheng, Jin Wen-Gang

Food and Machinery

Objective:This study aimed to analyze the differences of volatile compounds among fresh Gastrodia elata of different varieties and origins.Methods:Volatile components of fresh G. elata from six origin and three varieties were analyzed by gas chromatography-ion mobility spectrometry (GC-IMS) technology, and quantified and qualitatively from different angles using VOCal and Reporter, Gallery Plot and Dynamic PCA inserts and "nearest neighbor" fingerprint analysis.Results:In this study, a total of 75 kinds of volatile compounds were detected. Among them, 45 kinds of compounds were identified, including 16 aldehydes, 9 esters, 6 alcohols, 5 ketones and 3 acids. Principal component …

Optimization Of Preparation Technology And Antioxidant Activity Of Enzymatic Hydrolysates From Corneum Gigerii Galli Endothelium, Zhang Hui-Ying, Yang Wei-Qiao, Chen Yao, Li Pan, Du Bing

Food and Machinery

Objective:In order to study the optimal enzymatic hydrolysis process conditions of corneum galli gigerii endothelium, and analyze the amino acid composition, molecular weight distribution and antioxidant activity of the enzymatic hydrolysate.Methods:With the yield of polypeptide and the degree of hydrolysis as indicators, the orthogonal experiment was used to optimize the enzymatic hydrolysis conditions of corneum galli gigerii endothelium. Amino acid analyzer and high-performance gel permeation chromatography were used to determine the amino acid composition and molecular weight distribution of the enzymatic hydrolysate of corneum galli gigerii endothelium. The clear ability of corneum galli gigerii endothelium to DPPH …

Explore The Inhibitory Effect Of Perilla Crude Extract On Metabolic Syndrome-Related Enzymes Based On Molecular Docking, Yu Yu-Ting, Zhang Yanl, Zhang Ying, Li Na-Na, Cheng Yan, Zheng Meng-Di, Yan Ping, Chai Xi-Yan

Food and Machinery

Objective:The research is to explore the enzyme inhibition of Perilla crude extract, and to preliminarily elucidate the pharmacological substance basis of them on hyperglycemia, hyperuric acid and hyperlipidemia.Methods:The content of flavonoids and phenolic acid in UV extract was determined and showed the active components associated with enzyme inhibition. In vitro experiments, the inhibition of Perilla extracts on different enzyme activity was determined and the inhibitory activity was evaluated by IC50. Molecular docking was used to predict the targets of Perilla crude extract on pancreatic lipase and amylase inhibition.Results:Flavone content in the crude UV extract was …

Effects Of Extraction Methods On The Quality Of Elderberry Seed Oil, Xue Qing, Zhao Hong-Jin, Dai Long, Zhao Wen-Xin, Wang Jun, Zhu Xin-Liang, Gao Peng, Zhang Hong-Meng

Food and Machinery

Objective:This study aimed to compare the effects of different extraction methods including cold pressing extraction, soxhlet extraction and subcritical butane extraction on the yield, physicochemical properties, fatty acid composition and in vitro antioxidant activity of elderberry seed oil.Methods:Elderberry seed oils were extracted from elderberry seed by cold pressing extraction, soxhlet extraction and subcritical butane extraction. The physicochemical parameters and in vitro antioxidant activity of oil were determined with reference to national standards, and the fatty acid composition was analyzed by GC-MS.Results:Among the three extraction methods, the highest oil yield was obtained by subcritical butane extraction …

Advance In Research On Heat Treatment Mechanism Of Infrared Radiation And Its Application In Foods, Yu Xian-Long, Chu Bin, Xiao Hong-Wei, Zhang Wei-Peng, Zhao Feng, Zhang Zong-Chao

Food and Machinery

The development of food infrared heating theories were discussed in this review, and the advancement of infrared heating in food processing were summarized. Moreover, the principle of food processing and process optimum method in various food infrared thermal treatment technologies, including sterilizing, thawing, blanching, baking, peeling and drying processes were also discussed.

Research Progress On Industrial Auxiliary Materials Fermentation Of Cigar Tobacco Leaf, Ye Hui-Yuan, Ding Song-Shuang, Duan Wang-Jun, Huang Ming-Yue, Shi Xiang-Dong

Food and Machinery

This paper reviews the exogenous additives such as microorganisms, enzymes and excipients currently applied to cigar tobacco leaf fermentation and their research and application results to improve the quality of tobacco leaves, and prospects the research direction of cigar tobacco industrial excipient fermentation.

Research Progress On Composition And Processing Of Flos Lonicerae, Luo Lian, Ye Meng, Lin Jing, Chen An-Jun, Mo Han-Ru, Yu Jian-Bing, Liu Xing-Yan

Food and Machinery

In this paper, the research on the active components, dry processing methods, extraction of functional components and processed products of Flos Lonicerae at home and abroad in recent years were summarized and discussed, and the development direction of Flos Lonicerae in the future was prospected.

American Religion: A Study Of Religious Change From The 1920s Through 1970s, Alexander R. Marks-Katz

Masters Theses

Religion in America persisted along traditional Christian lines until the 1870s. It was then that theological liberalism gained significant headway. The Gilded Age and Progressive Era were still infused with revivals and preachers but there was a growing contingent that challenged the fundamentals of Christian belief. Sometimes this contingent supported revivals but promoted social causes and brought unorthodox biblical interpretations. At other times, they challenged traditional Christianity altogether. By the Great Depression, American culture had undergone such a tremendous amount of change that, faced with adversity, the bottom of religion fell out. Fewer people attended services and contributed funds. More …

Functional, Diagnostic And Therapeutic Aspects Of Bile, Monjur Ahmed

Division of Gastroenterology and Hepatology Faculty Papers

Bile is a unique body fluid synthesized in our liver. Enterohepatic circulation preserves bile in our body through its efficient synthesis, transport, absorption, and reuptake. Bile is the main excretory route for bile salts, bilirubin, and potentially harmful exogenous lipophilic substances. The primary way of eliminating cholesterol is bile. Although bile has many organic and inorganic contents, bile acid is the most physiologically active component. Bile acids have a multitude of critical physiologic functions in our body. These include emulsification of dietary fat, absorption of fat and fat-soluble vitamins, maintaining glucose, lipid, and energy homeostasis, sustenance of intestinal epithelial integrity …

Advanced Analytical Method Development: From Highly-Enrolled Classroom To Data-Intensive Proteomics, Laxmi Sinduri Vuppala

USF Tampa Graduate Theses and Dissertations

In analytical chemistry, method development is key to identifying, separating, and quantifying chemical components of interest systems. The chemical aspects are fundamental to understanding the subject of any analytical system, whether in academic research, industry, or government. These methodologies serve as a bridge between the classroom, where students are taught to be curious and enlightened, and research, where one strives to contribute. The Ph.D. dissertation integrates advanced methodologies in basic and applied research and advanced instrumentation, but the core criteria for validation remain the same. As part of chemical education curriculum development, a large cohort of undergraduates performed two gas …

4d Printing Of Smart Hydrogel Scaffold To Program Neural Stem Cell Differentiation, Omar Khater

USF Tampa Graduate Theses and Dissertations

Injuries like Traumatic brain injuries (TBI) and Spinal cord injuries (CSI) areconsidered one of the biggest challenges in the medical sector as there is no specific treatment for these injuries and the medical intervention on these injuries is restricted to reducing the pain and removing the damaged human parts. One of the helpful and most effective treatment methods for these injuries involve neural tissue regeneration in the area of the injury. The main issue when trying to treat such injuries is that neural tissues are often unable to regenerate by themselves and require outer human intervention to differentiate. Biomedical scaffolds …

Wetland Soil Development Along Salinity And Hydrogeomorphic Gradients In Active And Inactive Deltaic Basins Of Coastal Louisiana, Amanda Fontenot

LSU Master's Theses

Coastal wetlands provide an abundance of ecosystem services that benefit society, such as essential habitat for commercial species, storm protection, nutrient cycling, and carbon storage. Louisiana faces rapid rates of relative sea level rise (natural subsidence and eustatic sea levels) that threaten wetland survival, which are amplified by a reduction of riverine sediment input. An important determining factor of marsh survival is the formation of wetland platform elevation, known as vertical accretion, which is determined by several processes including sediment deposition & erosion, below ground biomass (BGB) productivity, decomposition of organic matter, shallow & deep subsidence, and soil compaction. Feldspar …

Isolation And Identification Of Pathogenic Fungi From Postharvest Blueberry In Sichuan Province And The Inhibition Effect On Them By Using Essential Oil, Liu Chun-Yan, Ding Jie, Deng Shang-Gui, Qin Wen, Liu Yan

Food and Machinery

Objective:This study aimed to provide strategy for controlling the fungal diseases of postharvest blueberry in Sichuan province.Methods:The pathogenic bacteria from 14 planting bases in Sichuan Province were isolate, identified, pathogenicity determination. Moreover, the effects of temperature and light on mycelia growth of the dominant and subdominant strains of blueberry after harvest were studied, and the effective natural antibacterial components against them were screened.Results:The blueberry postharvest rot diseases were caused by the following nine kinds of fungi:Aspergillus niger,Aspergillus tubingensis,Neofusicoccum parvum,Neopestalotiopsissp.,Fusarium graminearum,Alternaria alternata,Mucor fragilis,Penicilliumsp.,Cladosporium xylophilum.Aspergillus niger was the dominant species with the highest isolation frequency and the …

Research Progress On The Determination Of Free-Base Nicotine In Tobacco And Tobacco Products, Zhang Hao, Han Shu-Lei, Chen Huan, Hou Hong-Wei, Hu Qing-Yuan

Food and Machinery

The research progress on determination of free-base nicotine in tobacco and tobacco products in the past 30 years was reviewed and discussed, including solvent extraction method, headspace solid-phase microextraction method,1H NMR, Henderson-Hasselbalch method and spectroscopy method, etc. Advantages and limitations of each method were summarized and discussed and the development in this field was prospected.

Effects Of Soil Shrink-Swell On Bird Bones: An Experimental Taphonomy Pilot Study, Frank J. Dirrigl Jr., Adriana Diaz, Reyna Chavez

School of Integrative Biological & Chemical Sciences Faculty Publications

The soil burial context of a bird bone assemblage is affected by many different taphonomic factors. In this pilot study, we examined the potential effects of soil shrink-swell on white-tailed dove (Zenaida asiatica) bones experimentally buried in three soils of different clay contents. We predicted that bones buried in high clay soils would experience the most damage from soil shrink-swell. Dove wing and leg bones were buried and exposed to 60 days and eight episodes of cyclic soil shrink-swell. We recovered all bones (humeri, ulnae, radii, femora, and tibiotarsi) and found 77.1% of them to exhibit damage. …

"Don't Look Up" Your Science-Herd Immunity Or Herd Mentality?, Botond Z Igyártó

Department of Microbiology and Immunology Faculty Papers

This analysis piece will attempt to examine some of the critical pandemic-related measures implemented in the United States from an immunological perspective and pinpoint caveats that should have been considered before their implementation. I also discuss alternative measures grounded in scientific data that were not thoroughly explored and likely could have helped fight the pandemic.

Emotional Intelligence In Second Year Nursing Students: Measuring The Impact Of A Single Intervention, Tonya Anthony

Graduate Theses, Dissertations, and Capstones

ABSTRACT

Traditional second-year nursing students are often unprepared to manage their emotions, during clinical experiences, in volatile acute care settings. EI is emerging in the literature as an essential ability to foster effective interpersonal and interprofessional communication within the stressful confines of patient care settings. Furthermore, research shows that universities need to invest in the emotional development of students during the first few years of college to facilitate healthy psychosocial identities.

This study aimed to determine the impact of a single EI intervention on second-year students in a baccalaureate nursing program. Quantitative methods were utilized to measure statistical significance in …

Non-Destructive Classification And Quality Evaluation Of Proso Millet Cultivars Using Nir Hyperspectral Imaging With Machine Learning, Laruen E. Doyle, Julia R. Loeb, Nader Ekramirad, Dipak K. Santra, Akinbode A. Adedeji

Department of Agronomy and Horticulture: Faculty Publications

Millet is a small-seeded cereal crop with big potential and remarkable characteristics such as high drought resistance, short growing time, low water footprint, and the ability to grow in acidic soil. There is a need to develop nondestructive methods for differentiation and evaluation of the quality attributes of different of proso millet cultivars grown in the U.S. Current methods of cultivar classification are either subjective or destructive, time consuming, not allowing for the whole population to be tested, and requiring trained operators and special equipment. In this study, the feasibility of using near-infrared (NIR) hyperspectral imaging (900-1700 nm) to predict …

Development An Edge-Computing Sensing Unit For Continuous Measurement Of Canopy Cover Percentage Of Dry Edible Beans, Wei-Zhen Liang, Joseph Oboamah, Xin Qiao, Yufeng Ge, Robert M. Harveson, Daran R. Rudnick, Jun Wang, Haishun Yang, Angie Gradiz

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Canopy cover (CC) is an important indicator for crop development. Currently, CC can be estimated indirectly by measuring leaf area index (LAI), using commercially available hand-held meters. However, it does not capture the dynamics of CC. Continuous CC monitoring is essential for dry edible beans production since it can affect crop water use, weed, and disease control. It also helps growers to closely monitor “yellowness”, or senescence of dry beans to decide proper irrigation cutoff to allow the crop to dry down for harvest. The goal of this study was to develop a device – CanopyCAM, containing software and hardware …

Application Of Digital Twin In Evaluating Quality Changes In Tomato Value-Chain In Nigeria, Charles Nwaizu, Taofiq Olayinka Olanrewaju, Iluno Christiana

Department of Food Science and Technology: Faculty Publications

Cooling tomatoes after harvesting and keeping them refrigerated during their transportation is a key step toward preserving the quality attributes of tomatoes. This is a challenging task to accomplish in Nigeria, mainly because shipments of tomatoes from the tomato-producing states in northern Nigeria to the non-producing states in southern Nigeria were predominately not transported in a refrigerated ambient condition. To minimize postharvest losses of tomatoes during transportation, quantification of changes in quality would be extremely valuable. For this purpose, a digital tomato twin was developed based on mechanistic physics-based modeling. This digital twin simulated the actual thermal profile of tomatoes …

Effect Of Bromoform And Linseed Oil On Greenhouse Gas Emissions From Stored Beef Manure, Andrew Ortiz Balsero, Mara Zelt, Amy Millmier Schmidt, Miguel Fudolig, Daniel N. Miller

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Emissions of carbon dioxide, methane, and nitrous oxide – potent greenhouse gases - from stored beef feedlot manure are a significant concern relative to climate change. Research on methane reduction strategies for enteric emissions has identified the application of organic additives, such as bromoform and linseed oil, to ruminant diets as potential solutions for reducing enteric emissions and pathogenic bacteria in excreted manure. The objective of this study was to determine the effect of bromoform and linseed oil on greenhouse gas emissions from beef feedlot manure, and on E. coli concentration in beef cattle manure, during a 5-week storage period. …

Thoughts On Constructing Self-Reliance And Self-Strengthening Marine Scientific Observation And Detection Technology System, Yuantao Wu, Xiaobo Ren, Xiaonan Duan, Zhibin Wen, Danhong Dong, Jianping Yin, Zhongli Sha, Hongyu Zhao, Lei Jiang, Lixia Jiang, Gang Shen

Bulletin of Chinese Academy of Sciences (Chinese Version)

The marine area is a strategic area of China. To build China into a strong maritime country, we should enhance the capacity for exploiting marine resources, develop the maritime economy, protect the marine ecological environment, and resolutely safeguard national maritime rights and interests. Promoting marine science and technology to achieve high-level scientific and technological self-reliance is an inevitable requirement for accelerating the construction of maritime country. The marine scientific observation and exploration is the basic means of understanding the ocean, and is also an important basis for the marine resources development, environmental protection and rights and interests protection. This paper …