Digital Commons Network^™

Acetyl-Coenzyme A Synthetase 2 Potentiates Macropinocytosis And Muscle Wasting Through Metabolic Reprogramming In Pancreatic Cancer, Zhijun Zhou, Yu Ren, Jingxuan Yang, Mingyang Liu, Xiuhui Shi, Wenyi Luo, Kar-Ming Fung, Chao Xu, Michael S Bronze, Yuqing Zhang, Courtney W Houchen, Min Li

Faculty, Staff and Student Publications

BACKGROUND & AIMS: Rapid deconditioning, also called cachexia, and metabolic reprogramming are two hallmarks of pancreatic cancer. Acetyl-coenzyme A synthetase short-chain family member 2 (ACSS2) is an acetyl-enzyme A synthetase that contributes to lipid synthesis and epigenetic reprogramming. However, the role of ACSS2 on the nonselective macropinocytosis and cancer cachexia in pancreatic cancer remains elusive. In this study, we demonstrate that ACSS2 potentiates macropinocytosis and muscle wasting through metabolic reprogramming in pancreatic cancer.

METHODS: Clinical significance of ACSS2 was analyzed using samples from patients with pancreatic cancer. ACSS2-knockout cells were established using the clustered regularly interspaced short palindromic repeats-associated protein …

The Synapse: Issue 2022

The Synapse: Intercollegiate science magazine

No abstract provided.

Investigation Of Murine Host Sex As A Biological Variable In Epithelial Barrier Function And Muscle Contractility In Human Intestinal Organoids, Brooke T Beanland, Eoin P Mcneill, David J Sequeira, Hasen Xue, Noah F Shroyer, Allison L Speer

Faculty, Staff and Student Publications

Intestinal failure (IF) occurs when intestinal surface area or function is not sufficient to support digestion and nutrient absorption. Human intestinal organoid (HIO)-derived tissue-engineered intestine is a potential cure for IF. Research to date has demonstrated successful HIO transplantation (tHIO) into mice with significant in vivo maturation. An area lacking in the literature is exploration of murine host sex as a biological variable (SABV) in tHIO function. In this study, we investigate murine host SABV in tHIO epithelial barrier function and muscle contractility. HIOs were generated in vitro and transplanted into nonobese diabetic, severe combined immunodeficiency gamma chain deficient male …

The Equilibrium Of Tumor Suppression: Dubs As Active Regulators Of Pten, Audrey Christine, Mi Kyung Park, Su Jung Song, Min Sup Song

Faculty, Staff and Student Publications

PTEN is among the most commonly lost or mutated tumor suppressor genes in human cancer. PTEN, a bona fide lipid phosphatase that antagonizes the highly oncogenic PI3K-AKT-mTOR pathway, is considered a major dose-dependent tumor suppressor. Although PTEN function can be compromised by genetic mutations in inherited syndromes and cancers, posttranslational modifications of PTEN may also play key roles in the dynamic regulation of its function. Notably, deregulated ubiquitination and deubiquitination lead to detrimental impacts on PTEN levels and subcellular partitioning, promoting tumorigenesis. While PTEN can be targeted by HECT-type E3 ubiquitin ligases for nuclear import and proteasomal degradation, studies have …

Flow Cytometry Immunophenotypic Features Of Pure Erythroid Leukemia And The Distinction From Reactive Erythroid Precursors, Hong Fang, Sa A Wang, M James You, Shimin Hu, Roberto N Miranda, Zhenya Tang, Pei Lin, Jeffrey L Jorgensen, Jie Xu, Beenu Thakral, Ellen J Schlette, Siba El Hussein, Carlos Bueso-Ramos, L Jeffrey Medeiros, Wei Wang

Faculty, Staff and Student Publications

BACKGROUND: The immunophenotype of pure erythroid leukemia (PEL) as determined by flow cytometry immunophenotypic analysis is not well characterized. The immunophenotypic difference between PEL and reactive conditions is under-explored.

METHODS: We assessed and compared the immunophenotype of 24 PEL cases and 28 reactive cases containing early erythroid precursors by flow cytometry.

RESULTS: The neoplastic erythroid cells in all PEL cases were positive for CD36 and CD71. CD45 was also positive in all cases, but the expression level was often dimmer than granulocytes. CD117 expression ranged from partial to uniform, and CD235a was often only positive in the CD117-dim to negative …

Data Management Report November 2022, Tennessee. Department Of Intellectual And Developmental Disabilities

Compliance & System Data/Reports

This monthly report provides data on various aspects of the department's activities and programs.

The Role Of Nitrogen Fixation And Crop N Dynamics On Performance And Legacy Effects Of Maize-Grain Legumes Intercrops On Smallholder Farms In Tanzania, E. Mugi-Ngenga, L. Bastiaans, S. Zingore, N. P.R. Anten, K. E. Giller

All Peer-Reviewed Publications

Maize-grain legume intercrops form an important component of the cropping systems of smallholder farmers in many parts of sub-Saharan Africa. However, the effects of cropping system and fertilizer use on nitrogen fixation and nitrogen uptake of component crops in maize-legume intercrops are not well understood. Our study addressed the questions: (i) What is the capacity of pigeonpea and lablab to fix atmospheric nitrogen (N2) in sole crop and when intercropped with maize on smallholder farms across different agro-ecological conditions?; (ii) How does productivity and N-uptake by sole and intercropped maize and legumes respond to N and P fertilizer?; and (iii) …

Concurrent Jak2 V617f Acute Myeloid Leukemia (Aml) And Leukemic Non-Nodal Mantle Cell Lymphoma (Ln-Mcl): Case Study, Hovsep Ohan, Kedar Inamdar, Yulei Shen, Wei Liu, Juan C. Gomez-Gelvez, Sharmila Ghosh

Pathology Meeting Abstracts

Introduction/Objective: We report a unique case of concurrently occurring Acute Myeloid Leukemia (AML) and Leukemic non-nodal Mantle Cell Lymphoma (LN-MCL) in an 86-year-old male. To the best of our knowledge, this is the first report of AML occurring in the background of LN-MCL, with no known history of any malignancy or chemotherapy.

Methods/Case Report: An 86-year old Caucasian male with unremarkable past medical history presented with pancytopenia, fatigue and generalized weakness. Abdominal CT scan was negative for lymphadenopathy or hepatosplenomegaly. Peripheral blood showed 2% blasts with atypical lymphocytes 89%. Bone marrow aspirate showed 30% myeloblasts expressing CD34, CD117, CD13, CD33, …

Identifying Factors Associated With Periodontal Disease Using Machine Learning, Hussam M. Alqahtani, Siran M. Koroukian, Kurt C. Stange, Nicholas K. Schiltz, Nabil F. Bissada

Faculty Scholarship

Objective: This study aimed to identify combinations of chronic conditions associated with the presence and severity of periodontal disease (PD) after accounting for a series of demographic and behavioral characteristics in a nationally representative sample of US adults. Materials and Methods: A cross-sectional study of the 2013–2014 National Health and Nutrition Examination Survey (n = 4555). Outcome measure: PD using clinical attachment loss (measured as none, mild, moderate, or severe). The main independent variables were self-reported chronic conditions, while other covariates included demographic and behavioral variables. Classification and regression tree analysis was used to identify combinations of specific chronic conditions …

All-Day Prekindergarten: Closing The Achievement Gap In Literacy, Theodore Clevenger Iv

Electronic Theses and Dissertations

The purpose of this quantitative causal-comparative research study was to compare the impact that half-day and full-day prekindergarten had on economically disadvantaged students on Grade 3 State of Texas Assessments of Academic Readiness (STAAR) Reading test results. Data from students who previously attended a half-day and full-day prekindergarten program were analyzed to see which program had a greater impact on STAAR reading scores at the conclusion of third grade. The comparison included two urban school districts with an economically disadvantaged threshold of 70% or greater. Descriptive statistics to obtain frequencies were used to create data that showed how students performed …

Neurodevelopment And Recovery From Wasting, Harriet M. Babikako, Celine Bourdon, Emmie Mbale, Peace Aber, Annet Birabwa, Josephine Chimoyo, Wieger Voskuijl, Zaubina Kazi, John Mukisa, Ali Faisal Saleem

Department of Paediatrics and Child Health

Background and objectives: Acute illness with malnutrition is a common indication for hospitalization among children in low- and middle-income countries. We investigated the association between wasting recovery trajectories and neurodevelopmental outcomes in young children 6 months after hospitalization for an acute illness.
Methods: Children aged 2 to 23 months were enrolled in a prospective observational cohort of the Childhood Acute Illness & Nutrition Network, in Uganda, Malawi, and Pakistan between January 2017 and January 2019. We grouped children on the basis of their wasting recovery trajectories using change in mid-upper arm circumference for age z-score. Neurodevelopment was assessed with the …

Covid-19, Nutrition, And Gender: An Evidence-Informed Approach To Gender-Responsive Policies And Programs, Anna Kalbarczyk, Noora-Lisa Aberman, Bregje S M Van Asperen, Rosemary Morgan, Zulfiqar Ahmed Bhutta, Bianca Carducci, Rebecca Heidkamp, Saskia Osendarp, Neha Kumar, Anna Lartey

Department of Paediatrics and Child Health

In addition to the direct health impacts of COVID-19, government and household mitigation measures have triggered negative indirect economic, educational, and food and health system impacts, hitting low-and middle-income countries the hardest and disproportionately affecting women and girls. We conducted a gender focused analysis on five critical and interwoven crises that have emerged because of the COVID-19 crisis and exacerbated malnutrition and food insecurity. These include restricted mobility and isolation; reduced income; food insecurity; reduced access to essential health and nutrition services; and school closures. Our approach included a theoretical gender analysis, targeted review of the literature, and a visual …

Alleviating Hidden Hunger: An Infallible Bridge To Improved Health And Nutrition, Jai K. Das, Zahra Ali Padhani

Department of Paediatrics and Child Health

No abstract provided.

An Improved Bio-Based Activated Carbon For The Water Treatment Of A Lagoon In Northern Mexico Based On Life Cycle Assessment Methodology, Leticia Elizabeth Rodriguez

Open Access Theses & Dissertations

In this research, different options in the preparation of activated carbon are investigated looking at potential environmental impacts, production cost improvement and physical characteristics for water treatment. The activated carbon made with agricultural waste is compared to activated carbon made with hard coal. Life cycle assessment techniques follow ISO 14040 to 14044 standards are used and calculations were performed using GaBi _education 18® software and databases at mid-point TRACI impact categories. The results of the life cycle impact assessment show that, compared to activated carbon made with hard coal, activated carbon made with peanut shell and rice husk allows reductions …

Evolution Of Severe Acute Respiratory Coronavirus Virus 2 (Sars-Cov-2) Seroprevalence Among Employees Of A Us Academic Children’S Hospital During Coronavirus Disease 2019 (Covid-19) Pandemic, Brian T Fisher, Anna Sharova, Craig L K Boge, Sigrid Gouma, Audrey Kamrin, Jesse Blumenstock, Sydney Shuster, Lauren Gianchetti, Danielle Collins, Elikplim Akaho, Madison E Weirick, Christopher M Mcallister, Marcus J Bolton, Claudia P Arevalo, Eileen C Goodwin, Elizabeth M Anderson, Shannon R Christensen, Fran Balamuth, Audrey R Odom John, Yun Li, Susan Coffin, Jeffrey S Gerber, Scott E Hensley

Staff and Researcher Publications

Objective: To describe the cumulative seroprevalence of severe acute respiratory coronavirus virus 2 (SARS-CoV-2) antibodies during the coronavirus disease 2019 (COVID-19) pandemic among employees of a large pediatric healthcare system.

Design, setting, and participants: Prospective observational cohort study open to adult employees at the Children's Hospital of Philadelphia, conducted April 20-December 17, 2020.

Methods: Employees were recruited starting with high-risk exposure groups, utilizing e-mails, flyers, and announcements at virtual town hall meetings. At baseline, 1 month, 2 months, and 6 months, participants reported occupational and community exposures and gave a blood sample for SARS-CoV-2 antibody measurement by enzyme-linked immunosorbent assays …

Participatory Mapping To Address Neighborhood Level Data Deficiencies For Food Security Assessment In Southeastern Virginia, Usa, Nicole S. Hutton, George Mcleod, Thomas R. Allen, Christopher Davis, Alexander Garnand, Heather Richter, Prachi P. Chaven, Leslie Hoglund, Jill Comess, Matthew Herman, Brian Martin, Cynthia Romero

Political Science & Geography Faculty Publications

Background: Food is not equitably available. Deficiencies and generalizations limit national datasets, food security assessments, and interventions. Additional neighborhood level studies are needed to develop a scalable and transferable process to complement national and internationally comparative data sets with timely, granular, nuanced data. Participatory geographic information systems (PGIS) offer a means to address these issues by digitizing local knowledge.

Methods: The objectives of this study were two-fold: (i) identify granular locations missing from food source and risk datasets and (ii) examine the relation between the spatial, socio-economic, and agency contributors to food security. Twenty-nine subject matter experts from three cities …

Lactic Acid Bacteria Isolated From Fresh Vegetable Products: Potential Probiotic And Postbiotic Characteristics Including Immunomodulatory Effects, Fatima Khaled Alameri

Theses

The ability to perform effectively in the gastrointestinal system (GIT) is one of the most significant criteria for selecting the best probiotic bacteria. Thus, the present study aimed to investigate the potential probiotic characteristics of some selected lactic acid bacteria (LAB) isolated from vegetable products. Probiotic characteristics included tolerance to acid and bile, cholesterol removing ability, bile salt hydrolysis, resistance against lysozyme and antibiotics, production of exopolysaccharides (EPS), antimicrobial and hemolytic activities, and cell surface characteristics (auto-aggregation, co-aggregation, and hydrophobicity). Out of 46 isolates, 17 isolates that exhibited remarkable survivability under gastrointestinal conditions were selected for further analysis. Almost all …

Distribution Of Erm Proteins In The Stomach Mucosa Of Normal And Diabetic Rats, Asmaa Salem Al Sereidi

Theses

Diabetes mellitus (DM), or simply diabetes, refers to a group of metabolic disorders that include a variety of diseases that disrupt our body's metabolism. The long-term uncontrolled disease can lead to stomach complications such as gastroparesis, as well as disruption in several proteins, such as ERM (Ezrin, radixin, moesin) proteins. ERM proteins are three proteins that are structurally and functionally related. ERM proteins have molecular masses of 82, 80, and 75 kDa, respectively. Ezrin is highly expressed in the stomach, especially in parietal cells that secrete gastric acid. ERM proteins function as scaffolding proteins in lipid rafts and granule trafficking …

Utilizing Date Pits In Microencapsulation: Effect Of Different Variations On Probiotic Survivability Under In Vitro Digestion, Asmaa Samir Al Hamayda

Theses

This work deals with the medicinal use of date pit powder, which turns it from a waste product to a useful stabilizer for the release of beneficial probiotics in the human intestine. The main objective of this work is to encapsulate probiotics with date pit beads and their subsequent release in the human intestine. The beads were formed using gravitational dripping technique, while INFOGEST protocol was used to test the digested beads and the release of viable probiotic cells. For the three date varieties Raziz, Naghal and Khadrawy, it was found that 0.1 g of date pit powder per 0.2 …

Butyrate-Producing Bacteria And Insulin Homeostasis: The Microbiome And Insulin Longitudinal Evaluation Study (Miles), Jinrui Cui, Gautam Ramesh, Martin Wu, Elizabeth T Jensen, Osa Crago, Alain G Bertoni, Chunxu Gao, Kristi L Hoffman, Patricia A Sheridan, Kari E Wong, Alexis C Wood, Yii-Der I Chen, Jerome I Rotter, Joseph F Petrosino, Stephen S Rich, Mark O Goodarzi

Faculty, Staff and Students Publications

Gut microbiome studies have documented depletion of butyrate-producing taxa in type 2 diabetes. We analyzed associations between butyrate-producing taxa and detailed measures of insulin homeostasis, whose dysfunction underlies diabetes in 224 non-Hispanic Whites and 129 African Americans, all of whom completed an oral glucose tolerance test. Stool microbiome was assessed by whole-metagenome shotgun sequencing with taxonomic profiling. We examined associations among 36 butyrate-producing taxa (n = 7 genera and 29 species) and insulin sensitivity, insulin secretion, disposition index, insulin clearance, and prevalence of dysglycemia (prediabetes plus diabetes, 46% of cohort), adjusting for age, sex, BMI, and race. The genus Coprococcus …

Predictive Power Of 24-H Ambulatory Pulse Pressure And Its Components For Mortality And Cardiovascular Outcomes In 11 848 Participants Recruited From 13 Populations, Benjamin Gavish, Michael Bursztyn, Lutgarde Thijs, Dong-Mei Wei, Jesus Melgarejo, Zhen-Yu Zhang, José Boggia, Tine W. Hansen, Kei Asayama, Gladys E. Maestre

School of Medicine Publications

Background:

The role of pulse pressure (PP) ‘widening’ at older and younger age as a cardiovascular risk factor is still controversial. Mean PP, as determined from repeated blood pressure (BP) readings, can be expressed as a sum of two components: ‘elastic PP’ (elPP) and ‘stiffening PP’ (stPP) associated, respectively, with stiffness at the diastole and its relative change during the systole. We investigated the association of 24-h ambulatory PP, elPP, and stPP (‘PP variables’) with mortality and composite cardiovascular events in different age classes.

Method:

Longitudinal population-based cohort study of adults with baseline observations that included 24-h ambulatory BP. Age …

Assessing Urinary Phenol And Paraben Mixtures In Pregnant Women With And Without Gestational Diabetes Mellitus: A Case-Control Study, Wei-Jen Chen, Candace Robledo, Erin M. Davis, Jean R. Goodman, Chao Xu, Jooyeon Hwang, Amanda E. Janitz, Tabitha Garwe, Antonia M. Calafat, Jennifer D. Peck

School of Medicine Publications

Prior studies have identified the associations between environmental phenol and paraben exposures and increased risk of gestational diabetes mellitus (GDM), but no study addressed these exposures as mixtures. As methods have emerged to better assess exposures to multiple chemicals, our study aimed to apply Bayesian kernel machine regression (BKMR) to evaluate the association between phenol and paraben mixtures and GDM.

This study included 64 GDM cases and 237 obstetric patient controls from the University of Oklahoma Medical Center. Mid-pregnancy spot urine samples were collected to quantify concentrations of bisphenol A (BPA), benzophenone-3, triclosan, 2,4-dichlorophenol, 2,5-dichlorophenol, butylparaben, methylparaben, and propylparaben. Multivariable …

Triphenyl Phosphate Activates Estrogen Receptor Α/Nf-Κb/ Cyclin D1 Signaling To Stimulate Cell Cycle Progression In Human Ishikawa Endometrial Cancer Cells, Hyun Young Kwon, Seung Bin Park, Myoungseok Han, Jung-Woo Park, Yongmin Lee, Sang Jun Han, Youngmin Kwon, Yeon Jean Cho

Faculty, Staff and Students Publications

OBJECTIVE: Triphenyl phosphate (TPHP) is one of the most commonly used organophosphorus flame retardants that may accumulate in the environment. However, its effects on human reproductive organs have not been well studied. We aimed to investigate the in vitro effects of TPHP in human Ishikawa endometrial cancer cells to elucidate how TPHP exposure disrupts intracellular signaling and cell proliferation in reproductive tissues.

METHODS: Human Ishikawa endometrial cancer cells were exposed to TPHP.

RESULTS: Exposure to TPHP elevated the levels of estrogen receptor (ER) α and progesterone receptor-B and reduced ER β in human Ishikawa endometrial cancer cells. TPHP stimulated phosphoinositide …

Salpingitis In An Adolescent Female With Constipation And Abdominal Pain, Gal Barak, Matthew R Carroll, Andrea Dean

Faculty, Staff and Students Publications

Abdominal pain is one of the most common presenting complaints in the emergency room for pediatric patients. While constipation is one of the most common causes for abdominal pain in pediatrics, serious intra-abdominal pathology must always be excluded. We report a pre-coital post-menarchal adolescent female who presented with severe abdominal pain and constipation and had radiographic findings of salpingitis. It was suspected that uterine and adnexal changes seen on imaging resulted from the fecal mass compressing the genitourinary tract leading to fluid collection manifesting as radiographic evidence of salpingitis. This mechanism is similar to bladder outlet obstruction resulting from compression …

Resistant Starch As A Dietary Intervention To Limit The Progression Of Diabetic Kidney Disease, Anna M. Drake, Melinda T. Coughlan, Claus T. Christophersen, Matthew Snelson

Research outputs 2022 to 2026

Diabetes is the leading cause of kidney disease, and as the number of individuals with diabetes increases there is a concomitant increase in the prevalence of diabetic kidney disease (DKD). Diabetes contributes to the development of DKD through a number of pathways, including inflammation, oxidative stress, and the gut-kidney axis, which may be amenable to dietary therapy. Resistant starch (RS) is a dietary fibre that alters the gut microbial consortium, leading to an increase in the microbial production of short chain fatty acids. Evidence from animal and human studies indicate that short chain fatty acids are able to attenuate inflammatory …

Improving The Growth Performance Of The Mangrove Avicennia Marina Seedlings Using Plant Growth Promoting Actinobacteria, Alanoud Abdulla Al Shamsi

Theses

The combination of commercial fish emulsion (FE) and plant growth-promoting actinobacteria capable of producing plant growth regulators (PGRs) was evaluated as a biofertilizer to enhance growth and development of grey mangrove (Avicennia marina) in the United Arab Emirates. In vitro studies identified the rhizosphere-competent Streptomyces griseorubens UAE1 (Sg) that was able to solubilize phosphorus, produce auxins, cytokinins, gibberellic acid, polyamines, and 1-aminocyclopropane-1-carboxylic acid (ACC) deaminase. Growth promotion, nutrient contents and PGR levels in tissues of treated mangrove plants with fish emulsion and/or S. griseorubens UAE1 were further assessed in vivo. Under greenhouse and natural open-field nursery conditions, …

Enhancement Of Phenolic Recovery And Bioactivity Of Date Palm Leaf Extract Using Natural Deep Eutectic Solvents And Green Extraction Technology, Raghad Shehadeh Abdal Rahman

Theses

This study was designed to explore date palm leaves, an agro by-product, as a potential source of natural polyphenolic compounds using appropriate green Natural Deep Eutectic Solvent (NADES) and emerging green extraction technologies. Herein, six different NADES were prepared using six different hydrogen donors and choline chloride as hydrogen bond acceptor at a molar ratio of 1:2 (w/w) and different NADES concentrations (10, 20, 40, and 80%, in water, at 40°C). Among the studied NADES, Glycerol-based NADES showed higher phenolic yield compared to saccharide and organic acid-based NADES. Afterward, to enhance the extraction efficiency, NADES were coupled with microwave (MAE-NADES) …

Maternal Effects On Growth Parameters Of Chloris Gayana Under Co2 Enrichment, Ahmed Adnan Mohammed Mashli

Theses

Rhodes grass, Chloris gayana, is commonly used forage plant for grazers, livestock, and cattle. It significantly improves soil fertility and its overall structure by preventing nematode flourishment in soil. It is also used as a cover crop to upgrade soil health by reducing soil erosion and increasing water availability. The present study assessed the effects of CO₂ on the growth, development and maternal effects of C. gayana. The experimental setup was made in such a way that the potential impacts of CO₂ can be measured on the eco-physiological growth of C. gayana by studying successive generations. Generation 1 …

Screening For Endophytic Actinobacteria To Enhance Growth And Salinity Tolerance Of Tomato Plants In The United Arab, Amira Hamdy Hassan

Theses

Salinity is one of the most decisive environmental factors limiting the productivity of crop plants, mainly in arid and semi-arid regions. An eco-friendly technology can be used to boost crop production in saline areas by using plant growth-bacteria. Endophytic actinobacteria that produce the enzyme 1-Aminocyclopropane-1-Carboxylic acid (ACC) Deaminase (ACCD) can modulate the levels of Ethylene (ET) in plants to reduce the effect of abiotic stresses including high salt stress. The main objectives of this study were to: (1) Evaluate endophytic actinobacterial isolates from healthy tomato plants cultivated in the UAE that are capable of producing ACCD in vitro; (2) …

Acrylamide Mitigation By Novel Lactic Acid Bacteria And Bifidobacteria Isolated From Various Food Products: Optimization Of Mitigation, Effect Of Simulated Gastrointestinal Conditions, And Potential Mechanism, Amal Salem Albedwawi

Dissertations

Acrylamide is a toxic compound that is formed in heated carbohydrate-rich food. Scientists have analyzed acrylamide levels before, during, and after processing in order to study the mitigation strategies and, due to the lack of knowledge, this study aimed to: 1) screen 120 newly isolated LAB for their acrylamide removal; 2) optimize the conditions for 6 selected strains of pH, temperature, time and salt, using the Box-Behnken design (BBD), and analyze their acrylamide removal levels; 3) investigate acrylamide removal abilities of the selected LAB isolates under in-vitro digestion conditions, using the INFO-GEST 2.0 model; and 4) examine the mechanism of …