Digital Commons Network^™

Dependence On A Variable Residue Limits The Breadth Of An Hiv Mper Neutralizing Antibody, Despite Convergent Evolution With Broadly Neutralizing Antibodies, Cathrine Scheepers, Prudence Kgagudi, Nonkululeko Mzindle, Elin S. Gray, Thandeka Moyo-Gwete, Bronwen E. Lambson, Brent Oosthuysen, Batsirai Mabvakure, Nigel J. Garrett, Salim S.Abdool Karim, Lynn Morris, Penny L. Moore

Research outputs 2022 to 2026

Broadly neutralizing antibodies (bNAbs) that target the membrane-proximal external region (MPER) of HIV gp41 envelope, such as 4E10, VRC42.01 and PGZL1, can neutralize > 80 % of viruses. These three MPER-directed monoclonal antibodies share germline antibody genes (IGHV1 - 69 and IGKV3 - 20) and form a bNAb epitope class. Furthermore, convergent evolution within these two lineages towards a ^111.2GW^111.3 motif in the CDRH3 is known to enhance neutralization potency. We have previously isolated an MPER neutralizing antibody, CAP206 - CH12, that uses these same germline heavy and light chain genes but lacks breadth (neutralizing only 6 % of …

An Audit Of Sharps Injuries In Clinical Skills Simulation Wards At A Uk University, Kevin Hambridge, Ruth Endacott, Andrew Nichols

School of Nursing and Midwifery

Background/Aims Healthcare students are at high risk of sharps injuries, which can negatively impact their confidence and wellbeing. This study audited three clinical skills simulation wards at a UK university to determine the incidence of sharps injuries in this educational setting. Methods An audit of sharps injuries sustained in three clinical skills simulation wards was conducted, including data from 2008–2016. The authors developed an audit tool to collect data on the number of incidents that occurred, the type of individual involved and the nature of the incident. Results were analysed to compare the incidence of general injuries and sharps injuries …

Light Chain 2 Is A Tctex-Type Related Axonemal Dynein Light Chain That Regulates Directional Ciliary Motility In Trypanosoma Brucei, Subash Godar, James Oristian, Valerie Hinsch, Katherine Wentworth, Ethan Lopez, Parastoo Amlashi, Gerald Enverso, Samantha Markley, Joshua Daniel Alper

Open Access Publishing Fund

Flagellar motility is essential for the cell morphology, viability, and virulence of pathogenic kinetoplastids. Trypanosoma brucei flagella beat with a bending wave that propagates from the flagellum’s tip to its base, rather than base-to-tip as in other eukaryotes. Thousands of dynein motor proteins coordinate their activity to drive ciliary bending wave propagation. Dynein-associated light and intermediate chains regulate the biophysical mechanisms of axonemal dynein. Tctex-type outer arm dynein light chain 2 (LC2) regulates flagellar bending wave propagation direction, amplitude, and frequency in Chlamydomonas reinhardtii. However, the role of Tctex-type light chains in regulating T. brucei motility is unknown. Here, we …

Prescribing Controlled Substances Goes Electronic, Alberto Coustasse, Craig Kimble, Ken Maxik

Management Faculty Research

With the Affordable Care Act in 2010 came interoperability and meaningful use requirements. Part of these requirements included the implementation of electronic medical records (EMRs), which was a crucial part of achieving these standards. With EMRs, prescribers began sending electronic prescriptions. EMRs can provide advanced decision support when writing prescriptions, and they include features such as auto populating the quantity prescribed, formulary information, therapeutic duplications, warnings about interactions, or other potential clinical or regulatory issues. This system results in enhanced patient care and a more streamlined dispensing process. In addition, electronic prescriptions for controlled substances (EPCS) has become more widely …

Promoting Validation And Cross-Phylogenetic Integration In Model Organism Research., Keith C Cheng, Rebecca D Burdine, Mary E Dickinson, Stephen C Ekker, Alex Y Lin, K C Kent Lloyd, Cathleen Lutz, Calum A Macrae, John H Morrison, David H O'Connor, John H Postlethwait, Crystal D Rogers, Susan Sanchez, Julie H Simpson, William S Talbot, Douglas C Wallace, Jill M Weimer, Hugo J Bellen

Faculty Research 2022

Model organism (MO) research provides a basic understanding of biology and disease due to the evolutionary conservation of the molecular and cellular language of life. MOs have been used to identify and understand the function of orthologous genes, proteins, cells and tissues involved in biological processes, to develop and evaluate techniques and methods, and to perform whole-organism-based chemical screens to test drug efficacy and toxicity. However, a growing richness of datasets and the rising power of computation raise an important question: How do we maximize the value of MOs? In-depth discussions in over 50 virtual presentations organized by the National …

Genetically Diverse Mouse Platform To Xenograft Cancer Cells, Jennifer K Sargent, Mark A. Warner, Benjamin E. Low, William H. Schott, Todd Hoffert, David Coleman, Xing Yi Woo, Todd B Sheridan, Sonia Erattupuzha, Philipp P Henrich, Vivek M. Philip, Jeffrey Chuang, Michael V. Wiles, Muneer G. Hasham

Faculty Research 2022

The lack of genetically diverse preclinical animal models in basic biology and efficacy testing has been cited as a potential cause of failure in clinical trials. We developed and characterized five diverse RAG1 null mouse strains as models that allow xenografts to grow. In these strains, we characterized the growth of breast cancer, leukemia and glioma cell lines. We found a wide range of growth characteristics that were far more dependent on strain than tumor type. For the breast cancer cell line, we characterized the spectrum of xenograft/tumor growth at structural, histological, cellular and molecular levels across each strain, and …

2022-2023 Catalog, College Of The Holy Cross

Course Catalogs

This catalog lists available courses for the 2022-2023 academic year. The College Catalog is a document of record issued in September 2022.The Catalog contains current information regarding the College calendar, admissions, degree requirements, fees, regulations and course offerings. It is not intended to be, and should not be relied upon, as a statement of the College’s contractual undertakings.

Why Angiotensin Ii Is A Poor Choice For Circulatory Support Of Ventilated Covid-19 Patients Compared To Vasopressin, Robert C. Speth, Michael Bader

HPD Articles

Early in the COVID-19 pandemic when it was first reported that SARS-CoV-2 used membrane-bound angiotensin-converting enzyme-2 (ACE2) as its receptor for entry into cells, warnings were raised against the use of angiotensin-converting enzyme (ACE) inhibitors and angiotensin receptor blockers (ARBs) because of their potential to increase ACE2 expression. These reports ignored the adverse effects that the renin-angiotensin system (RAS) exerts on the cardiovascular system and kidneys via its primary hormone angiotensin (Ang) II acting upon AT receptors that could exacerbate the cytokine storm induced by SARS-CoV-2 . At one point it was even recommended that COVID-19 patients suffering from cardiovascular …

Comprehensive Evidence Implies A Higher Social Cost Of Co2, Kevin Rennert, Frank Errickson, Brian C. Prest, Lisa Rennels, Richard G. Newell, William Pizer, Cora Kingdon, Jordan Wingenroth, Roger Cooke, Bryan Parthum, David Smith, Kevin Cromar, Delavane Diaz, Frances C. Moore, Ulrich K. Müller, Richard J. Plevin, Adrian E. Raftery, Hana Ševčíková, Hannah Sheets, James H. Stock, Tammy Tan, Mark Watson, Tony E. Wong, David Anthoff

Articles

The social cost of carbon dioxide (SC-CO2) measures the monetized value of the damages to society caused by an incremental metric tonne of CO2 emissions and is a key metric informing climate policy. Used by governments and other decision-makers in beneft–cost analysis for over a decade, SC-CO2 estimates draw on climate science, economics, demography and other disciplines. However, a 2017 report by the US National Academies of Sciences, Engineering, and Medicine1 (NASEM) highlighted that current SC-CO2 estimates no longer refect the latest research. The report provided a series of recommendations for improving the scientifc basis, transparency and uncertainty characterization of …

Macomb Dental Society Journal Fall 2022

Journal of the Macomb Dental Society

In the Fall 2022 issue, the reader will find the following feature articles:

• Young Dentists
• The Macomb Oakland Detroit Meeting
• The General Meeting

Also included are regular columns from the President, Executive Director and Editor, as well as news shorts and information on upcoming events.

Addition Of Retinoic Acid-Inducible Gene 1 To Ebola Virus-Like Particles To Enhance Virus-Like Particle Vaccines, Elizabeth Pautz

Student Research and Creative Projects 2021-2022

The long-term goal of this study is to investigate if human monocytes treated with Ebola virus (EBOV) virus-like particles (VLPs) supplemented with a constitutively active form of retinoic acid-inducible gene 1 (caRIG-I) induce an inflammatory response in vitro.

Knowledge And Practice Of Pediatricians In Kenya Regarding Helicobacter Pylori Infection In Children., Aura Mwende Nzinga

Master of Medicine in Paediatrics and Child Health

Background: Helicobacter pylori is one of the most common chronic bacterial infections. It affects more than half of the world’s population and is frequently associated with peptic ulcer disease, gastric carcinoma and other extra gastrointestinal manifestations. Globally, one third of the children are infected with the highest burdens observed in low and middle income countries. In Kenya the prevalence stands at 87% among school going children and 50% in children under three years. Justification: Studies in high income countries suggest poor knowledge and practice amongst physicians with limited data from Africa. There exists conflicting information regarding the pathogenic role and …

Immunosuppressant Responsive Neuropsychiatric Systemic Lupus Erythematosus Manifestations Initially Diagnosed As Schizophrenia And Bipolar Disorder, Benjamin D. Goodman, Tala Al-Saghir, Harsha Alles, Rami M. Youssef, Hesham Shaban

Internal Medicine Articles

Systemic lupus erythematosus (SLE) is a well-documented multi-system autoimmune disease with increased frequency noted in younger females and among minority populations. Disease-defining signs and symptoms can vary widely and involve multiple organ systems including the nervous system. Involvement of the nervous system, known as neuropsychiatric SLE (NPSLE) can present as manifestations consistent with central nervous system or peripheral nervous system pathologies, with the former including presentations of psychiatric illnesses. This case report reviews a 21-year-old Black female's presentation that was most notable for psychosis with other findings on examination and laboratory investigation resulting in a diagnosis of NPSLE. Our patient …

Eaton Selected As Nam Emerging Leader In Health And Medicine Scholar, Adam Pope

Browse All News

No abstract provided.

Clinical Practice Guidelines From The Association For The Advancement Of Blood And Biotherapies (Aabb): Covid-19 Convalescent Plasma, Lise J. Estcourt, Claudia S. Cohn, Monica B. Pagano, Claire Iannizzi, Nina Kreuzberger, Nicole Skoetz, Elizabeth S. Allen, Evan M. Bloch, Gregory Beaudoin, Arturo Casadevall, Dana V. Devine, Farid Foroutan, Thomas J. Gniadek, Ruchika Goel, Jed Gorlin, Brenda J. Grossman, Michael J. Joyner, Ryan A. Metcalf, Jay S. Raval, Todd W. Rice, Ralph R. Vassallo, Jeffrey L. Winters, Aaron A R Tobian, Beth H. Shaz

Pathology Research and Scholarship

DESCRIPTION: Coronavirus disease 2019 convalescent plasma (CCP) has emerged as a potential treatment of COVID-19. However, meta-analysis data and recommendations are limited. The Association for the Advancement of Blood and Biotherapies (AABB) developed clinical practice guidelines for the appropriate use of CCP.

METHODS: These guidelines are based on 2 living systematic reviews of randomized controlled trials (RCTs) evaluating CCP from 1 January 2019 to 26 January 2022. There were 33 RCTs assessing 21 916 participants. The results were summarized using the GRADE (Grading of Recommendations Assessment, Development and Evaluation) method. An expert panel reviewed the data using the GRADE framework …

Nsf Supported Socio-Environmental Research: How Do Crosscutting Programs Affect Research Funding, Publication, And Citation Patterns?, Kendra E. Kaiser, Anna E. Braswell, Megan L. Fork

Geosciences Faculty Publications and Presentations

Recognizing the continued human domination of landscapes across the globe, social-ecological systems (SES) research has proliferated, necessitating interdisciplinary collaborations. Although interdisciplinary research started gaining traction in academic settings close to 50 years ago, formal frameworks for SES research did not develop until the late 1990s. The first National Science Foundation (NSF) funding mechanism specifically for interdisciplinary SES research began in 2001 and the SES-specific Coupled Natural Human (CNH) Systems program began in 2007. We used data on funded NSF projects from 2000 to 2015 to examine how SES research was funded, where the research is published, and the scholarly impact …

Communication Skills Of General Practitioners In Nairobi, Kenya: A Descriptive Observational Study, Gulnaz Mohamoud, Robert Mash

Family Medicine, East Africa

Background High-quality primary care needs to be person-centred, and GPs must communicate effectively to ensure continuity and coordination of care. In Kenya, there is little knowledge about the quality of communication in consultations by GPs.

Aim To evaluate the quality of communication in consultations by GPs.

Design & setting Descriptive, observational study of 23 GP consultations in 13 private sector primary care facilities in Nairobi, Kenya.

Method One consultation with a randomly selected adult patient was recorded per GP, and 16 communication skills evaluated with the Stellenbosch University Observation Tool (SUOT). A total percentage score was calculated per consultation, and …

Estimating R0 From Early Exponential Growth: Parallels Between 1918 Influenza And 2020 Sars-Cov-2 Pandemics, Grant Foster, Bret D. Elderd, Robert L. Richards, Tad Dallas

Faculty Publications

The large spatial scale, geographical overlap, and similarities in transmission mode between the 1918 H1N1 influenza and 2020 SARS-CoV-2 pandemics together provide a novel opportunity to investigate relationships between transmission of two different diseases in the same location. To this end, we use initial exponential growth rates in a Bayesian hierarchical framework to estimate the basic reproductive number, R0, of both disease outbreaks in a common set of 43 cities in the United States. By leveraging multiple epidemic time series across a large spatial area, we are able to better characterize the variation in R0 across the United States. Additionally, …

Ambient Temperature During Pregnancy And Risk Of Maternal Hypertensive Disorders: A Time-To-Event Study In Johannesburg, South Africa, Chérie Part, Jean Le Roux, Matthew Chersichb, Shobna Sawry, Véronique Filippi, Nathalie Roos, Lee Fairlie, Britt Nakstad, Jeroen De Bont, Stanley Luchters

Institute for Human Development, East Africa

Hypertensive disorders in pregnancy are a leading cause of maternal and perinatal mortality and morbidity. We evaluate the effects of ambient temperature on risk of maternal hypertensive disorders throughout pregnancy. We used birth register data for all singleton births (22–43 weeks' gestation) recorded at a tertiary-level hospital in Johannesburg, South Africa, between July 2017–June 2018. Time-to-event analysis was combined with distributed lag non-linear models to examine the effects of mean weekly temperature, from conception to birth, on risk of (i) high blood pressure, hypertension, or gestational hypertension, and (ii) pre-eclampsia, eclampsia, or HELLP (hemolysis, elevated liver enzymes, low platelets). Low …

Estimating R0 From Early Exponential Growth: Parallels Between 1918 Influenza And 2020 Sars-Cov-2 Pandemics, Grant Foster, Bret D. Elderd, Robert L. Richards, Tad Dallas

Faculty Publications

The large spatial scale, geographical overlap, and similarities in transmission mode between the 1918 H1N1 influenza and 2020 SARS-CoV-2 pandemics together provide a novel opportunity to investigate relationships between transmission of two different diseases in the same location. To this end, we use initial exponential growth rates in a Bayesian hierarchical framework to estimate the basic reproductive number, R₀, of both disease outbreaks in a common set of 43 cities in the United States. By leveraging multiple epidemic time series across a large spatial area, we are able to better characterize the variation in R₀ across the …

Function Of Atm And Msh2 During Dna Repair And Recombination, Emily Sible

Dissertations, Theses, and Capstone Projects

Class switch recombination (CSR) produces secondary immunoglobulin isotypes and requires AID-dependent DNA deamination of intronic switch (S) regions within the immunoglobulin heavy chain (Igh) gene locus. Non-canonical repair of deaminated DNA by mismatch repair (MMR) or base excision repair (BER) creates DNA breaks that permit recombination between distal S regions. ATM-dependent phosphorylation of AID at serine-38 (pS38-AID) promotes its interaction with APE1, a BER protein, suggesting that ATM regulates CSR through BER. However, pS38-AID may also function in MMR during CSR, although the mechanism remains unknown. To examine whether ATM modulates BER- and/or MMR-dependent CSR, Atm^-/- mice …

The Making Of A Bilingual University In The 21st Century, Michael Mena

Dissertations, Theses, and Capstone Projects

At the southernmost tip of Texas, the University of Texas Rio Grande Valley (UTRGV) opened its doors on August 31, 2015 as a ‘bilingual, bicultural, and biliterate’ campus—the only one of its kind and at a scale never before attempted in the United States. This is a categorical achievement in the near 200 year-long quest for the educational advancement of Latinxs in Texas—a state historically structured by white supremacist ideologies, violent economic and political disenfranchisement, as well as a racially segregated education system designed to maintain exploitative labor practices (Montejano 1987; González 1990, 2013, 1999; Blanton 2004). This constitutes the …

Geography, Niches, And Transportation Influence Bovine Respiratory Microbiome And Health, Jianmin Chai, Xinting Liu, Hunter Usdrowski, Feilong Deng, Ying Li, Jiangchao Zhao

Animal Science Faculty Publications and Presentations

Bovine respiratory disease (BRD), one of the most common and infectious diseases in the beef industry, is associated with the respiratory microbiome and stressors of transportation. The impacts of the bovine respiratory microbiota on health and disease across different geographic locations and sampling niches are poorly understood, resulting in difficult identification of BRD causes. In this study, we explored the effects of geography and niches on the bovine respiratory microbiome and its function by re-analyzing published metagenomic datasets and estimated the main opportunistic pathogens that changed after transportation. The results showed that diversity, composition, structure, and function of the bovine …

Pause. Reset. Return. Mental Hygiene And Mindfulness In Daily Routines For The Mental Wellbeing Of Mental Health Professionals, Jamie Tolson

Mindfulness Studies Theses

Children and adolescents in the United States are experiencing a mental health crisis which also impacts families, caregivers, including the mental health professionals providing support. This rationale paper expands on the history and limited current research to reintroduce the term “mental hygiene” as a lens to address the ongoing stress, the need for self-care and respite, and the value of incorporating mindfulness in the daily routines of mental health professionals. Intentional use of the term “mental hygiene" seeks to highlight an active and engaged process including mindfulness practices to decrease stress and burnout. The literature supports the inclusion of mindfulness …

Understanding Distruptive Change In Health Care: Perspectives From The Front Lines Of Covid, Wendy Vaughon

Dissertations and Theses

COVID-19 dramatically stressed an already overburdened healthcare system. Physicians, susceptible to burnout, bore the brunt of this threat as frontline providers. Limited data are available describing how the pandemic may have affected physician perceptions and beliefs, including experiences of burnout and plans to exit the field. Reddit, a social media website, allows physicians the ability to interact with their peers pseudonymously on a purpose-driven, publicly viewable subreddit (r/medicine). By providing a space for “physicians and other medical professionals” to interact, it is possible to observe unfiltered discourse.

This dissertation explores physician-peer discourse on Reddit prior to and during the COVID-19 …

The Role Of Booster Vaccination And Ongoing Viral Evolution In Seasonal Circulation Of Sars-Cov-2, A.N.M. Kraay, M.E. Gallagher, Y. Ge, P. Han, J.M. Baker, K. Koelle, A. Handel, B.A. Lopman

Faculty Publications

Periodic resurgences of COVID-19 in the coming years can be expected, while public health interventions may be able to reduce their intensity. We used a transmission model to assess how the use of booster doses and non-pharmaceutical interventions (NPIs) amid ongoing pathogen evolution might influence future transmission waves. We find that incidence is likely to increase as NPIs relax, with a second seasonally driven surge expected in autumn 2022. However, booster doses can greatly reduce the intensity of both waves and reduce cumulative deaths by 20% between 7 January 2022 and 7 January 2023. Reintroducing NPIs during the autumn as …

Genetic Structure Correlates With Ethnolinguistic Diversity In Eastern And Southern Africa, Elizabeth G. Atkinson, Shareefa Dalvie, Yakov Pichkar, Allan Kalungi, Lerato Majara, Anne Stevenson, Tamrat Abebe, Dickens Akena, Melkam Alemayehu, Lukoye Atwoli

Brain and Mind Institute

African populations are the most diverse in the world yet are sorely underrepresented in medical genetics research. Here, we examine the structure of African populations using genetic and comprehensive multi-generational ethnolinguistic data from the Neuropsychiatric Genetics of African Populations-Psychosis study (NeuroGAP-Psychosis) consisting of 900 individuals from Ethiopia, Kenya, South Africa, and Uganda. We find that self-reported language classifications meaningfully tag underlying genetic variation that would be missed with consideration of geography alone, highlighting the importance of culture in shaping genetic diversity. Leveraging our uniquely rich multi-generational ethnolinguistic metadata, we track language transmission through the pedigree, observing the disappearance of several …

A Qualitative Descriptive Study On Re-Assessing The Mental Certification By Faa For Future Pilots, Kaung Z. Min

Student Works

Mental illness becomes one of the main problems that most pilots do not usually address. It is not because pilots do not have the courage or are open enough to talk with someone, but because the Federal Aviation Administration (FAA) forces them to hide from mental depression. Most of the time, the pilots are not willing to declare such illnesses as they fear losing their job; simultaneously, the Federal Aviation Agencies across the world require pilots to be in peat health, including their mental condition, to operate the aircraft. While it can be said that the passengers’ and crews’ safety …

A Prognostic Model Of All-Cause Mortality At 30 Days In Patients With Cancer And Covid-19, S. Halabi, B. Luo, H. Dzimitrowicz, Clara Hwang, T. M. Wise-Draper, C. Labaki, R. R. Mckay, E. Ruiz, C. Rangel-Escareño, D. Farmakiotis, E. A. Griffiths, C. T. Jani, M. Accordino, C. Friese, E. Wulff-Burchfield, M. Puc, P. Yu, U. Topaloglu, S. Mishra, J. Warner

Hematology/Oncology Meeting Abstracts

Background: Patients with cancer are at higher risk of dying of COVID-19. Known risk factors for 30-day all-cause mortality (ACM-30) in patients with cancer are older age, sex, smoking status, performance status, obesity, and co-morbidities. We hypothesized that common clinical and laboratory parameters would be predictive of a higher risk of 30-day ACM, and that a machine learning approach (random forest) could produce high accuracy.

Methods: In this multi-institutional COVID-19 and Cancer Consortium (CCC19) registry study, 12,661 patients enrolled between March 17, 2020 and December 31, 2021 were utilized to develop and validate a model of ACM-30. ACM-30 was defined …

Chth 491.01: Principles Of Epidemiology, Erin O'Brien Semmens

University of Montana Course Syllabi, 2021-2025

No abstract provided.