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Clinicopathological Study Of Oral Plasma Cell Granuloma, Aya M. El-Bastawesi, Mona M. Ibrahim, Nadia A. El-Sissy

Mansoura Journal of Dentistry

Objective: To identify the characteristic clinical and Histopathologic features encountered in the oral lesions of PCG and thus, clarifying the variability nature of the studied oral plasma cell granulomas (OPCGS). Materials and Methods a sample of 25 retrospective paraffin blocks of the OPCGS were thoroughly investigated histopathologically. Results: the studied OPCG was mainly represented as a gingival swelling, there was a female tendency in most of the studied OPCGS (92%), ranging from 11-73 years with a mean of 42.40. the majority of the current OPCG (64%) did not express any bone changes. the OPCGS of the present work were characterized …

Immunohistochemical Expression Of Ezh2 In Mucoepidermoid Carcinoma Of Salivary Glands, Amira A. Mansour, Mona M. Ibrahim, Ahmed E. Ahmed, Azza A. El Sissi

Mansoura Journal of Dentistry

Objective: To disclose the variation in EZH2 expression among the different grades of MEC and to analyze the marker's immunohistochemical (IHC) expression in relation to the clinicopathologic data of all cases. Material and methods: The present study was conducted on 15 blocks diagnosed as MEC including equal numbers of different grades. These blocks were selected from the archives of the Oncology Center, Mansoura University. The specimens embedded in the retrieved paraffin blocks were all fixed in 10% formalin. Sections of 4 microns’ thickness intervals were serially cut and prepared for the EZH2 staining. Chi-square, Monte Carlo, and Fisher Exact tests …

Effect Of Food Simulating Solvents On Surface Roughness And Microhardness Of Bulk Fill Resin Composites., Hadeer M. Hal, Ashraf I. Ali, Noha A. El-Wassefy, Marmar A. Montaser

Mansoura Journal of Dentistry

Objectives: To evaluate the effect of food simulating solvents on surface roughness and microhardness of bulk fill resin composites in-vitro. Materials and methods: A total of 180 discs were made with (Filtek One bulk fill, REVEAL HD and x-tra fil bulk fill (n=60). The baseline data of surface roughness and microhardness measurements were recorded. Each group was divided into three subgroups, subgroup1 was immersed into methyl ethyl ketone (n=20), subgroup2 was immersed into ethanol (n=20) and, subgroup3 was immersed into artificial saliva (n=20) as control group. After 30 days, half of each subgroup was tested for surface roughness (n=10) by …

Mdpb-Containing Self-Etch Dental Adhesive: Antibacterial Activity And Effect Of Different Dentinal Surface Preparation, Sally N. Zaki, Ragy N. Saad, Hamdi H. Hamdan, Salah H. Mahmoud

Mansoura Journal of Dentistry

Objective: To evaluate the effect of MDBP-containing self-etch (SE) adhesive and different dentin surface pretreatments on antibacterial activity Materials and Methods: A total of forty disc shaped specimens fabricated (8 mm in diameter and 1.5 mm thickness) were divided into four main groups according to type of the surface treatment method; deionized water(control), CHX, Papin enzyme and Riboflavin. Each group was sub-divided into 2subgroups (n=5) according to the type of adhesive: classic or MDPB containing SE adhesives. Bonded specimens were stored in deionized water for 24 hr. (n=6). The plates contained dices shaped specimen was incubated at 37o C for …

Earlier Snowmelt May Lead To Late Season Declines In Plant Productivity And Carbon Sequestration In Arctic Tundra Ecosystems, Donatella Zona, Peter M. Lafleur, Koen Hufkens, Barbara Bailey, Beniamino Gioli, George Burba, Jordan P. Goodrich, Anna K. Liljedahl, Eugénie S. Euskirchen, Jennifer D. Watts, Mary Farina, John S. Kimball, Martin Heimann, Mathias Göckede, Martijn Pallandt, Torben R. Christensen, Mikhail Mastepanov, Efrén López-Blanco, Marcin Jackowicz-Korczynski, Albertus J. Dolman, Luca Belelli Marchesini, Roisin Commane, Steven C. Wofsy, Charles E. Miller, David A. Lipson, Josh Hashemi, Kyle A. Arndt, Lars Kutzbach, David Holl, Julia Boike, Christian Wille, Torsten Sachs, Aram Kalhori, Xia Song

Daugherty Water for Food Global Institute: Faculty Publications

Arctic warming is affecting snow cover and soil hydrology, with consequences for carbon sequestration in tundra ecosystems. The scarcity of observations in the Arctic has limited our understanding of the impact of covarying environmental drivers on the carbon balance of tundra ecosystems. In this study, we address some of these uncertainties through a novel record of 119 site-years of summer data from eddy covariance towers representing dominant tundra vegetation types located on continuous permafrost in the Arctic. Here we found that earlier snowmelt was associated with more tundra net CO2 sequestration and higher gross primary productivity (GPP) only in June …

Lee Kong Chian School Of Business Year In Review 2021-2022, Singapore Management University

SMU Corporate Reports

As Singapore and the world reopens, this has led to changes, especially as people feel safe to travel abroad again. This has impacted us of course. We have seen an 8.9 percent drop in undergraduate applications from our 2022 high. This decline is due in no small part to the relaxation of travel restrictions. However, despite the drop, the quality of applications this year is similar or maybe even slightly better than the year before. In the end, we matriculated 884 students, above our target. This includes 38 scholarship recipients who accepted our offer, representing a record yield. We have …

Predictions Of Indentation Stiffness Of Musculoskeletal Regions Using Ultrasound, Sean Doherty

ETD Archive

Tissue indentation response is an important metric for understanding how different musculoskeletal regions respond to loading and is a function of the tissue’s form. Modem imaging techniques provide information about the internal structures of human tissue. Ultrasound remains one of the most common imaging techniques performed, given its portability and low costs. Prior work and data collection on 100 patients involved the collection of ultrasound images at eight different locations across the musculoskeletal extremities. Given the tissue structure information that the medical imaging provided, it was hypothesized that the mechanical properties of the tissue could be predicted from this data. …

Final Master's Portfolio, Marisa Mount

Master of Arts in English Plan II Graduate Projects

The following portfolio project contains four separate pieces of writing produced during the author's MA coursework.

Rad51-Mediated Interhomolog Recombination During Budding Yeast Meiosis Is Promoted By The Meiotic Recombination Checkpoint And The Conserved Pif1 Helicase, Andrew Ziesel, Qixuan Weng, Jasvinder S. Ahuja, Abhishek Bhattacharya, Raunak Dutta, Evan Cheng, G. Valentin Borner Ph.D., Michael Lichten, Nancy M. Hollingsworth

Biological, Geological, and Environmental Faculty Publications

During meiosis, recombination between homologous chromosomes (homologs) generates crossovers that promote proper segregation at the first meiotic division. Recombination is initiated by Spo11-catalyzed DNA double strand breaks (DSBs). 5' end resection of the DSBs creates 3' single strand tails that two recombinases, Rad51 and Dmc1, bind to form presynaptic filaments that search for homology, mediate strand invasion and generate displacement loops (D-loops). D-loop processing then forms crossover and noncrossover recombinants. Meiotic recombination occurs in two temporally distinct phases. During Phase 1, Rad51 is inhibited and Dmc1 mediates the interhomolog recombination that promotes homolog synapsis. In Phase 2, Rad51 becomes active …

Singapore Management University Report To Stakeholders 2021/2022, Singapore Management University

Report to Stakeholders

The title of the annual report, “Our Evolving Garden”, points to how the University continues to develop and progress as “One SMU”. With the world now learning to live with COVID-19 as endemic, the last year has been one in which SMU students have been able to resume many of the activities that contribute towards providing a transformative education. For example, they were able to attend many more events on campus and to travel overseas for internships, exchanges, and community service projects. Faculty too were able to attend academic conferences and meet with educational partners, business connections and research associates …

Mars 2100: A Microcosm Of Ecocentric Design, Andrea Hoe

Architecture Thesis Prep

In the dystopian future of 2100, Earth becomes a dying planet and humanity is forced to continue colonizing the solar system for survival. Mars develops as a colonized planet, exploited for its natural resources and potential. The hierarchical system of power seen constantly throughout Earth’s history was inescapable, and poor conditions turned to extremes. As billionaires take control of the space race, the capitalist system reflected in past company towns was implemented, making the moral questions and lines blurred.

When the system is eventually ruptured, ecosystemic living is proposed. Living is now centered around health and mindfulness, creating a system …

Paradigm Of The Post Natural: Critiquing Capitalist Ideals Through Environmental Degradation, Andrea De Haro, Charlotte Bascombe

Architecture Thesis Prep

There is an unspoken value in the destructiveness caused by design that is exposed through the exploitation of natural resources. This thesis seeks to exemplify the aesthetic value inherent within these newly defined environments. Nature is a source for human consumption, and as such it has developed into a commodity. By now it should be made evident that humanity’s presence is everywhere. There is no ecosystem left unturned by human manipulation Iin this respect, nature is dead. Deforested sites of oil fracking leave mile long toxic ponds highlighting the destructive pursuits that capitalism creates. Chernobyl showcases how human errors in …

Tri-Ag News Academics Research Extension College Of Agriculture Sciences - Spring/Summer 2022, Prairie View A&M University

Prairie View Magazine Collection

No abstract provided.

Lifestyle Interventions For Prevention And Management Of Diet-Linked Non-Communicable Diseases Among Adults In Arab Countries, Maryam Naveed Muhammad Tariq, Lily Stojanovska, Ayesha S. Al Dhaheri, Leila Cheikh Ismail, Vasso Apostolopoulos, Habiba I. Ali

CoMHS Faculty Work

The increased incidences of diet-related non-communicable diseases (NCDs) such as diabetes, obesity, and cardiovascular diseases among adults are becoming the chief public health concern in most Arab countries. Economic expansion has contributed to a nutrition shift from a traditional seasonal diet to Westernized eating habits coupled with a sedentary lifestyle. Despite the rising concern for NCD mortality, public health policies are inadequately addressed. This narrative review aims to discuss the effectiveness of nutritional interventions focusing on diet and physical activity in the management of NCDs among Arab adults. A comprehensive literature search was performed using different database platforms such as …

Tlr4/Nfκb-Mediated Anti-Inflammatory And Antioxidative Effect Of Hexanic And Ethanolic Extracts Of Curcuma Longa L. In Buffalo Mammary Epithelial Cells, Insha Amin, Shahzada Mudasir Rashid, Sheikh Shubeena, Ishraq Hussain

Faculty and Student Publications

Mastitis is the commonest disease of bovines imposing a great economic setback and a drastic public health concern worldwide. Antibiotic resistance is the preliminary con of excessive antibiotics use for disease treatment. Studies validate an alarming increase in the antibiotic resistance of both humans and animals. For ages, medicinal plants have been used to treat a number of ailments in humans and animals, including mastitis. Curcuma longa, or turmeric, is the prerequisite in Indian cooking and has been used in traditional medicine for its magical properties. The rhizome of C. longa possesses significant anti-inflammatory, antibacterial, antifungal, and antioxidative properties. In …

Immunogenetic Role Of Il17a Polymorphism In The Pathogenesis Of Recurrent Miscarriage, Shafat Ali, Sabhiya Majid, Md Niamat Ali, Mujeeb Zafar Banday

Faculty and Student Publications

Interleukin-17A (IL17A) is a proinflammatory cytokine and is assumed to play an important role in fetal rejection. In order to evaluate the potential role of IL17A polymorphism in the pathogenesis of recurrent miscarriage (RM), serum IL17A levels were estimated by ELISA. Single-nucleotide polymorphism was assessed by polymerase chain reaction-restriction fragment-length polymorphism (PCR-RFLP) using gene-specific primers and the EcoNI restriction enzyme. Serum IL17A levels were nonsignificantly (p > 0.5) low in RM patients compared with the control group. IL17A gene amplification by PCR yielded the undigested product of 815 bp, and its digestion with EcoNI enzyme produced 815, 529, 286, and 270 …

Antitrypanosomal, Antitopoisomerase-I, And Cytotoxic Biological Evaluation Of Some African Plants Belonging To Crassulaceae; Chemical Profiling Of Extract Using Uhplc/Qtof-Ms/Ms, Mostafa M. Hegazy, Wael M. Afifi, Ahmed M. Metwaly, Mohamed M. Radwan

Faculty and Student Publications

In our continuous study for some African plants as a source for antitrypanosomally and cytotoxic active drugs, nine different plants belonging to the Crassulaceae family have been selected for the present study. Sedum sieboldii leaves extract showed an antitrypanosomal activity against Trypanosoma brucei with an IC50 value of 8.5 µg/mL. In addition, they have cytotoxic activities against (HCT-116), (HEPG-2) and (MCF-7), with IC50 values of 28.18 ± 0.24, 22.05 ± 0.66, and 26.47 ± 0.85 µg/mL, respectively. Furthermore, the extract displayed inhibition against Topoisomerase-1 with an IC50 value of 1.31 µg/mL. It showed the highest phenolics and flavonoids content among …

Isolation And Characterization Of Flavonoid Naringenin And Evaluation Of Cytotoxic And Biological Efficacy Of Water Lilly (Nymphaea Mexicana Zucc.), Shajrath Din, Saima Hamid, Aadil Yaseen, Ali Mohd Yatoo

Faculty and Student Publications

Despite its limited exploration, Nymphaea mexicana Zucc. can be beneficial if pharmacology, isolation, and biological evaluation are given attention. It is an aquatic species that belongs to the family Nymphaeaceae. The thrust area of the work was the extraction, isolation, and biological evaluation of different extracts of the N. mexicana Zucc. plant. The primary goal of this research was to assess the antimicrobial, antioxidant, and anticancer activities of the extracts and to isolate the target naringenin compound. Comparative FT IR analysis of different extracts of this plant revealed the presence of functional groups of plant secondary metabolites, including polyphenols, flavonoids, …

Bioassay-Guided Fractionation With Antimalarial And Antimicrobial Activities Of Paeonia Officinalis, Mamdouh Nabil Samy, Basma Khalaf Mahmoud, Nourhan Hisham Shady, Usama Ramadan Abdelmohsen

Faculty and Student Publications

Bioassay-guided fractionation technique of roots of Paeonia officinalis led to isolation and structure elucidation of seven known compounds, including four monoterpene glycosides: lactiflorin (1), paeoniflorin (4), galloyl paeoniflorin (5), and (Z)-(1S,5R)-β-pinen-10-yl β-vicianoside (7); two phenolics: benzoic acid (2) and methyl gallate (3); and one sterol glycoside: β-sitosterol 3-O-β-D-glucopyranoside (6). The different fractions and the isolated compounds were evaluated for their antimicrobial and antimalarial activities. Fraction II and III showed antifungal activity against Candida neoformans with IC50 values of 28.11 and 74.37 µg/mL, respectively, compared with the standard fluconazole (IC50 = 4.68 µg/mL), and antibacterial potential against Pseudomonas aeruginosa (IC50 = …

Cannabidiol Loaded Topical Ophthalmic Nanoemulsion Lowers Intraocular Pressure In Normotensive Dutch-Belted Rabbits, Samir Senapati, Ahmed Adel Ali Youssef, Corinne Sweeney, Chuntian Cai

Faculty and Student Publications

Cannabidiol (CBD) is the major non-psychoactive and most widely studied of the cannabinoid constituents and has great therapeutic potential in a variety of diseases. However, contradictory reports in the literature with respect to CBD’s effect on intraocular pressure (IOP) have raised concerns and halted research exploring its use in ocular therapeutics. Therefore, the current investigation aimed to further evaluate CBD’s impact on the IOP in the rabbit model. CBD nanoemulsions, containing Carbopol® 940 NF as a mucoadhesive agent (CBD-NEC), were prepared using hot-homogenization followed by probe sonication. The stability of the formulations post-moist-heat sterilization, in terms of physical and chemical …

Abcg2 Polymorphisms And Predictive Fluoroquinolone Phototoxicity In Nondomestic Felids, Alexandria E. Gochenauer, Dayna L. Dreger, Brian W. Davis, Shawna Cook

Faculty and Student Publications

Fluoroquinolones are a widely used class of chemotherapeutics within veterinary medicine, prized for their broad-spectrum bactericidal activity. These drugs present a known risk of retinal phototoxicity in domestic cats (Felis catus); therefore, using lower doses and alternative antibiotic classes is encouraged in this species. This adverse drug effect of fluoroquinolones, and enrofloxacin specifically, has been determined to be species-specific in domestic felids. Four feline-specific missense variants in ABCG2 result in four amino acid changes (E159M, S279L, H283Q, and T644I) that are unique to the domestic cat compared with multiple other nonfeline mammalian species. These changes alter the ABCG2 protein involved …

How Carbon Nanoparticles, Arbuscular Mycorrhiza, And Compost Mitigate Drought Stress In Maize Plant: A Growth And Biochemical Study, Emad A. Alsherif, Omar Almaghrabi, Ahmed M. Elazzazy, Mohamed Abdel-Mawgoud

Faculty and Student Publications

Drought negatively affects crop growth and development, so it is crucial to develop practical ways to reduce these consequences of water scarcity. The effect of the interactive potential of compost (Comp), mycorrhizal fungi (AMF), and carbon nanoparticles (CNPS) on plant growth, photosynthesis rate, primary metabolism, and secondary metabolism was studied as a novel approach to mitigating drought stress in maize plants. Drought stress significantly reduced maize growth and photosynthesis and altered metabolism. Here, the combined treatments Com-AMF or Com-AMF-CNPs mitigated drought-induced reductions in fresh and dry weights. The treatments with AMF or CNPS significantly increased photosynthesis (by 10%) in comparison …

Discovery Of A Lectin Domain That Regulates Enzyme Activity In Mouse N-Acetylglucosaminyltransferase-Iva (Mgat4a), Masamichi Nagae, Tetsuya Hirata, Hiroaki Tateno, Sushil K. Mishra

Faculty and Student Publications

N-Glycosylation is a common post-translational modification, and the number of GlcNAc branches in N-glycans impacts glycoprotein functions. N-Acetylglucosaminyltransferase-IVa (GnT-IVa, also designated as MGAT4A) forms a β1-4 GlcNAc branch on the α1-3 mannose arm in N-glycans. Downregulation or loss of GnT-IVa causes diabetic phenotypes by dysregulating glucose transporter-2 in pancreatic β-cells. Despite the physiological importance of GnT-IVa, its structure and catalytic mechanism are poorly understood. Here, we identify the lectin domain in mouse GnT-IVa’s C-terminal region. The crystal structure of the lectin domain shows structural similarity to a bacterial GlcNAc-binding lectin. Comprehensive glycan binding assay using 157 glycans and solution NMR …

Milk-Compositional Study Of Metabolites And Pathogens In The Milk Of Bovine Animals Affected With Subclinical Mastitis, Aarif Ali, Manzoor Ur Rahman Mir, Showkat Ahmad Ganie, Saima Mushtaq

Faculty and Student Publications

Bovine milk is an important food component in the human diet due to its nutrient-rich metabolites. However, bovine subclinical mastitis alters the composition and quality of milk. In present study, California mastitis testing, somatic cell count, pH, and electrical conductivity were used as confirmatory tests to detect subclinical mastitis. The primary goal was to study metabolome and identify major pathogens in cows with subclinical mastitis. In this study, 29 metabolites were detected in milk using gas chromatography–mass spectrometry. Volatile acidic compounds, such as hexanoic acid, hexadecanoic acid, lauric acid, octanoic acid, n-decanoic acid, tricosanoic acid, tetradecanoic acid, and hypogeic acid …

Validated Determination Of Nrg1 Ig-Like Domain Structure By Mass Spectrometry Coupled With Computational Modeling, Niloofar Abolhasani Khaje, Alexander Eletsky, Sarah E. Biehn, Charles K. Mobley

Faculty and Student Publications

High resolution hydroxyl radical protein footprinting (HR-HRPF) is a mass spectrometry-based method that measures the solvent exposure of multiple amino acids in a single experiment, offering constraints for experimentally informed computational modeling. HR-HRPF-based modeling has previously been used to accurately model the structure of proteins of known structure, but the technique has never been used to determine the structure of a protein of unknown structure. Here, we present the use of HR-HRPF-based modeling to determine the structure of the Ig-like domain of NRG1, a protein with no close homolog of known structure. Independent determination of the protein structure by both …

Prescribing Trends Of Proton Pump Inhibitors, Antipsychotics And Benzodiazepines Of Medicare Part D Providers, Jennifer M. Toth, Saumil Jadhav, Holly M. Holmes, Manvi Sharma

Faculty and Student Publications

Background: Proton pump inhibitors, benzodiazepines, and antipsychotics are considered potentially inappropriate medications in older adults according to the American Geriatric Society Beers Criteria, and deprescribing algorithms have been developed to guide use of these drug classes. The objective of this study was to describe the number of beneficiaries prescribed these medications, provider specialty and regional trends in prescribing, and the aggregate costs for these claims in Medicare Part D. Methods: This was a retrospective cross-sectional study using publicly available Medicare Provider Utilization and Payment Data: Part D Prescriber data for years 2013–2019. Descriptive statistics and the Cochrane-Armitage test were used …

Utility Of Fatty Acid Profile And In Vitro Immune Cell Activation For Chemical And Biological Standardization Of Arthrospira/Limnospira, Jungmoo Huh, Jin Zhang, Radka Hauerová, Joseph Lee

Faculty and Student Publications

Commercially cultivated Limnospira (species formerly classified to genus Arthrospira) is a popular food/supplement consumed by millions of people worldwide for health benefits. The objective of the current research was to advance the standardization technology for Limnospira. Quantitative methods were established to detect fatty acids as potential chemical markers and immune-enhancing activity. Analysis of 20 different batches of biomass obtained from one commercial grower demonstrated that there was a statistically significant relationship between the sum of two fatty acids (linoleic and γ-linolenic) and Toll-like receptor (TLR)2/TLR1-dependent activation (R2 = 0.48, p = 0.0007). Investigation of 12 biomass samples sourced from growers …

Comparative Pharmacokinetics And Tissue Distribution Of Primaquine Enantiomers In Mice, Pius S. Fasinu, Narayan D. Chaurasiya, N. P. Dhammika Nanayakkara, Yan‑Hong ‑H Wang

Faculty and Student Publications

Background: Primaquine (PQ) has been used for the radical cure of relapsing Plasmodium vivax malaria for more than 60 years. PQ is also recommended for prophylaxis and prevention of transmission of Plasmodium falciparum. However, clinical utility of PQ has been limited due to toxicity in individuals with genetic deficiencies in glucose 6-phosphate dehydrogenase (G6PD). PQ is currently approved for clinical use as a racemic mixture. Recent studies in animals as well as humans have established differential pharmacological and toxicological properties of the two enantiomers of PQ. This has been attributed to differential metabolism and pharmacokinetics of individual PQ enantiomers. The …

A Distinct Mechanism Of C-Type Inactivation In The Kv-Like Kcsa Mutant E71v, Ahmed Rohaim, Bram J. A. Vermeulen, Jing Li, Felix Kümmerer

Faculty and Student Publications

C-type inactivation is of great physiological importance in voltage-activated K+ channels (Kv), but its structural basis remains unresolved. Knowledge about C-type inactivation has been largely deduced from the bacterial K+ channel KcsA, whose selectivity filter constricts under inactivating conditions. However, the filter is highly sensitive to its molecular environment, which is different in Kv channels than in KcsA. In particular, a glutamic acid residue at position 71 along the pore helix in KcsA is substituted by a valine conserved in most Kv channels, suggesting that this side chain is a molecular determinant of function. Here, a combination of X-ray crystallography, …

Psychometric Properties Of The 26-Item Eating Attitudes Test (Eat-26): An Application Of Rasch Analysis, Natalie M. Papini, Myungjin Jung, Amanda Cook, Nanette V. Lopez, Lauren T. Ptomey, Stephen D. Herrmann, Minsoo Kang

Faculty and Student Publications

Background: The 26-item Eating Attitudes Test (EAT-26) is a commonly used tool to assess eating disorder risk. The purpose of this study was to examine the psychometric properties of the EAT-26 with a combined sample: (1) of adults with overweight and obesity enrolled in a behavioral weight loss program and (2) general adult sample (n = 469; age = 36.17 ± 17.83 years; female = 72.5%; white = 66.3%; obese BMI category = 58%). Methods: Rasch modeling was used to assess model-data fit, create an item-person map to evaluate relative distribution items and persons, item difficulty, and person’s eating disorder …