Digital Commons Network^™

Neovasculogenic Effect Of 11,12-Epoxyeicosatrienoic Acid Involves The Akt/Enos Signaling Pathways In Human Endothelial Progenitor Cells, Hung-Chang Hung, Jia-Ning Syu, Che-Yi Chao, Shu-Ming Huang, Cheng-Chieh Lin, Mei-Due Yang, Shu-Yao Tsai, Feng-Yao Tang

BioMedicine

The 11,12-epoxy-eicosatrienoic acid (11,12-EET) is formed from arachidonic acid (AA) by cytochrome P450 2J2 (CYP 2J2) epoxygenase and function as an effector in blood vessels. Human endothelial progenitor cells (hEPCs), a preceding cell source for endothelial cells (ECs), involve in the vascular tissue repairing by postnatal neovasculogenesis. However, the effect of 11, 12-EET on hEPCs and neovasculogenesis is not well known. In the current study, we examined the function of 11, 12-EET in hEPCs-mediated neovasculogenesis by using tubular formation analysis, Western Blotting assay, immunofluorescence staining, flow cytometry analysis and zymogram analysis. The results suggest that 11, 12-EET significantly induces neovasculogenesis …

Misunderstanding Misunderstanding: Exploring The Depth Of Hindrance To Cross-Cultural Communications, Geoff Beech

University Faculty Publications and Creative Works

Those who have endeavored to engage cross-culturally understand that the process is neither simple nor straightforward. Apart from the obvious problems associated with understanding and misunderstanding a different language, at times we fall into the trap of not comprehending the real depth of our cultural misunderstanding, which in turn inhibits our engagement with those in the other culture and the effectiveness of our ministries. This article examines some of the deeper aspects of engagement relating to alterity, relational directionality, occupied “spaces,” pretheoretical assumptions, and the fusion of horizons of understanding.

Ncoa4 Regulates Iron Recycling And Responds To Hepcidin Activity And Lipopolysaccharide In Macrophages, Cole A. Guggisberg, Juyoung Kim, Jaekwon Lee, Xiaoli Chen, Moon-Suhn Ryu

Department of Biochemistry: Faculty Publications

Macrophages, via erythrophagocytosis, recycle iron from effete erythrocytes to newly developing red blood cells. Conversion of potentially cytotoxic levels of iron from its heme into nonheme form during iron recycling is safely accomplished via coordinated regulations of cellular iron transport and homeostasis. Herein, we demonstrate the roles and regulation of NCOA4 (nuclear receptor coactivator 4)-mediated ferritinophagy in macrophages after erythrophagocytosis using the mouse macrophage cell line J774 cells. Ferritin in J774 cells increased with the rise of nonheme iron by erythrocyte ingestion and declined when total cellular iron contents subsequently decreased. NCOA4, a selective autophagic cargo receptor for ferritin, was …

Biofilms: Formation, Research Models, Potential Targets, And Methods For Prevention And Treatment, Yajuan Su, Jaime T. Yrastorza, Mitchell Matis, Jenna Cusick, Siwei Zhao, Guangshun Wang, Jingwei Xie

Department of Mechanical and Materials Engineering: Faculty Publications

Due to the continuous rise in biofilm-related infections, biofilms seriously threaten human health. The formation of biofilms makes conventional antibiotics ineffective and dampens immune clearance. Therefore, it is important to understand the mechanisms of biofilm formation and develop novel strategies to treat biofilms more effectively. This review article begins with an introduction to biofilm formation in various clinical scenarios and their corresponding therapy. Established biofilm models used in research are then summarized. The potential targets which may assist in the development of new strategies for combating biofilms are further discussed. The novel technologies developed recently for the prevention and treatment …

Scaling Cellular Agriculture Production With Photolithography: A Study On Curing And Degradation, Alexis Garrett

Department of Mechanical and Materials Engineering: Dissertations, Theses, and Student Research

To meet the requirements for full production in cellular agriculture, a multi-disciplinary approach is required. New questions and understandings between different concepts, such as texture and cooking, need to be identified and how they correlate with cellular behavior and taste. This work focuses on understanding and developing photolithographic systems for scalable cellular agriculture. There were two specific research directions: 1) a traditional photolithography printing system was improved upon for mass manufacturing purposes and 2) mechanical analysis and degradation of key potential photolithographic biomaterials were tested for a food product. A novel digital rotational ultraviolet manufacturing (DRUM) process was developed, and …

Apoptosis Detection In Retinal Ganglion Cells Using Quantitative Changes In Multichannel Fluorescence Colocalization, Xudong Qiu, Seth T Gammon, James R Johnson, Federica Pisaneschi, Steven W Millward, Edward M Barnett, David Piwnica-Worms

2020-Current year OA Pubs

KcapTR488 is a dual-fluorophore peptide sensor for the real-time reporting of programmed cell death by fluorescence imaging. KcapTR488 contains a nuclear localization sequence (NLS) conjugated with Texas Red, a caspase-cleavable sequence (DEVD), and a C-terminus conjugated to Alexa Fluor 488 (AF488). The synthesis and preliminary evaluation in cellulo of KcapTR488 for monitoring cell death by fluorescence imaging has been previously reported, but its utility in vivo has yet to be tested or validated. Herein, in vitro solution experiments verified the intramolecular fluorescence resonance energy transfer (FRET) between the two fluorophores and enabled a quantitative analysis of enzyme rates and selectivity. …

Development Of Siap Antisipasi (Sianti) To Enhance Students’ Assertive Behavior In Preventing Physical Sexual Harassment On Junior High School Students, Fitri Ratnasari, Elia Flurentin, Arbin Janu Setiyowati

Jurnal Kajian Bimbingan dan Konseling

This research and development aims to develop a product called Siap Antisipasi (SIANTI) to improve assertive behavior in preventing physical sexual harassment in junior high school students by meeting product acceptance. SIANTI’s products include three educational videos with a video duration of three minutes each and a guidebook for school counselors in providing classical guidance services. The research method by adapting Borg and Gall model. This research and development procedure includes preliminary studies, product design and development as well as product testing of material experts, media experts and users. The research and development subjects consisted of one material expert, one …

Reflections On Beauty, Miklos Nikolaus Legrady

Contemporary Aesthetics (Journal Archive)

No abstract provided.

Streaming Property, Lee Anne Fennell

Northwestern University Law Review

People acquire property rights in objects and real estate in order to capture the stream of services that these assets can provide over time. The thing or parcel itself is merely a delivery mechanism, a way of packaging and protecting rights to that value stream. And, significantly, these assets cannot stream services to anyone without a set of facilitating conditions and complementary goods, such as public infrastructure, that do not lie within the asset owner’s individual control. This Essay argues that we can gain fresh traction on inequality by recasting property as service streams rather than as owned things. Doing …

American Courts' Image Of A Tenant, Nadav Shoked

Northwestern University Law Review

What is the core of current American residential landlord–tenant law, and how was that core formed? This Essay argues that in the past few decades courts have settled on a two-pronged landlord–tenant law regime. The law provides tenants with assurances respecting the quality of the units they rent. It does not, conversely, provide them with any assurances respecting the price of the rental units—and, therefore, respecting their ability to remain in those units.

The first component of the regime was established through the well-known judicial creation and endorsement of the warranty of habitability. The second component’s entrenchment is often attributed …

Organization Of The Gravity-Sensing System In Zebrafish, Zhikai Liu, David G C Hildebrand, Joshua L Morgan, Yizhen Jia, Nicholas Slimmon, Martha W Bagnall

2020-Current year OA Pubs

Motor circuits develop in sequence from those governing fast movements to those governing slow. Here we examine whether upstream sensory circuits are organized by similar principles. Using serial-section electron microscopy in larval zebrafish, we generated a complete map of the gravity-sensing (utricular) system spanning from the inner ear to the brainstem. We find that both sensory tuning and developmental sequence are organizing principles of vestibular topography. Patterned rostrocaudal innervation from hair cells to afferents creates an anatomically inferred directional tuning map in the utricular ganglion, forming segregated pathways for rostral and caudal tilt. Furthermore, the mediolateral axis of the ganglion …

Phenotypic And Genetic Spectrum Of Atp6v1a Encephalopathy: A Disorder Of Lysosomal Homeostasis, Renzo Guerrini, Davide Mei, Katalin Kerti-Szigeti, Sara Pepe, Mary Kay Koenig, Gretchen Von Allmen, Megan T Cho, Kimberly Mcdonald, Janice Baker, Vikas Bhambhani, Zöe Powis, Lance Rodan, Rima Nabbout, Giulia Barcia, Jill A Rosenfeld, Carlos A Bacino, Cyril Mignot, Lillian H Power, Catharine J Harris, Dragan Marjanovic, Rikke S Møller, Trine B Hammer, Riikka Keski Filppula, Päivi Vieira, Clara Hildebrandt, Stephanie Sacharow, Luca Maragliano, Fabio Benfenati, Katherine Lachlan, Andreas Benneche, Florence Petit, Jean Madeleine De Sainte Agathe, Barbara Hallinan, Yue Si, Ingrid M Wentzensen, Fanggeng Zou, Vinodh Narayanan, Naomichi Matsumoto, Alessandra Boncristiano, Giancarlo La Marca, Mitsuhiro Kato, Kristin Anderson, Carmen Barba, Luisa Sturiale, Domenico Garozzo, Roberto Bei, Laura Masuelli, Valerio Conti, Gaia Novarino, Anna Fassio

Faculty, Staff and Student Publications

Vacuolar-type H+-ATPase (V-ATPase) is a multimeric complex present in a variety of cellular membranes that acts as an ATP-dependent proton pump and plays a key role in pH homeostasis and intracellular signalling pathways. In humans, 22 autosomal genes encode for a redundant set of subunits allowing the composition of diverse V-ATPase complexes with specific properties and expression. Sixteen subunits have been linked to human disease. Here we describe 26 patients harbouring 20 distinct pathogenic de novo missense ATP6V1A variants, mainly clustering within the ATP synthase α/β family-nucleotide-binding domain. At a mean age of 7 years (extremes: 6 weeks, youngest deceased …

The Complete Mitochondrial Genome Of Talpa Martinorum (Mammalia: Talpidae), A Mole Species Endemic To Thrace: Genome Content And Phylogenetic Considerations, S Demirtas, Mahir Budak, Ertan M. Korkmaz, Jeremy B. Searle, David T. Bilton, I Gunduz

School of Biological and Marine Sciences

The complete mitogenome sequence of Talpa martinorum, a recently described Balkan endemic mole, was assembled from next generation sequence data. The mitogenome is similar to that of the three other Talpa species sequenced to date, being 16,835 bp in length, and containing 13 protein-coding genes, two ribosomal RNA genes, 22 transfer RNA genes, an origin of L-strand replication, and a control region or D-loop. Compared to other Talpa mitogenomes sequenced to date, that of T. martinorum differs in the length of D-loop and stop codon usage. TAG and T-- are the stop codons for the ND1 and ATP8 genes, respectively, …

Augmented Reality-Based English Language Learning: Importance And State Of The Art, Mohammad Wedyan, Jannat Falah, Omar Elshaweesh, Salsabeel F. M. Alfalah, Moutaz Alazab

All Works

Augmented reality is increasingly used in the educational domain. However, little is known concerning the actual importance of AR for learning English skills. The weakness of the English language among English as a foreign Language (EFL) students is widespread in different educational institutions. Accordingly, this paper aims at exploring the importance of AR for learning English skills from the perspectives of English language teachers and educators. Mixed qualitative methods were used. To achieve the objective of this study, 12 interviews were conducted with English teachers concerning the topic under investigation. Second, a systematic literature review (SLR) that demonstrates the advantages, …

A Theoretical Framework For M-Learning, Osman Sadeck, Moses Moyo, Nyarai Tunjera, Agnes Chigona

African Conference on Information Systems and Technology

The proliferation of mobile technologies in all spheres of society necessitates looking at their beneficial use for education. Many parts of South Africa (SA), Africa and the world experience social-exclusion and marginalisation in education. Education is a human right, and this paper looks at using mobile devices as a means to counter E-exclusion. Emphasis is currently on the adoption and use of mobile technologies, available in many communities, for offsetting E-inclusion. This paper presents an evaluation of mobile learning (M-learning) and M-learning models towards a theoretical framework for M-learning for marginalised contexts. The model offered is applicable to a range …

Road Traffic Accidents Severity Modelling In Malawi, Chikumbutso Felix Phiri, Kondwani Munthali, Yamikani Phiri

African Conference on Information Systems and Technology

One of the significant problems Malawi faces today is the rate at which road traffic accidents and deaths are happening on the roads of Malawi. It is very crucial to effectively address such a problem with a limited budget considering that Malawi is a developing country. To supplement the current safety measures, traffic accidents data mining using machine learning models was considered. Being able to predict the severity of an accident as well as determining the weight each attribute contributes to the severity could help authorities make informed decisions. Therefore, this research aimed at modeling the severity of road accidents …

Using Design Science Research To Create A Virtual Environment For Nursing Students, Benjamin Botha, Lizette De Wet

African Conference on Information Systems and Technology

This paper aims to provide insight into the design science research approach followed during a transdisciplinary research study between computer scientists and professional nurses. The researchers used a design science research approach to create a virtual environment where nursing students could practice managing a patient with a foreign object in the right lung. The virtual environment underwent multiple changes as it progressed through the different cycles of design science research. Within these cycles, namely the relevance, design, and rigor cycle, various iterations of each of the cycles took place. Presented in this paper the design science approach followed will be …

The Epistemology Of Complexity And "Doing" Interdisciplinarity, Sharon Woodill

Interface: The C3 Lab Knowledge Commons

Complexity is now well established as the driver of interdisciplinarity, but what this means for how knowledge is ‘done’ within Interdisciplinary Studies has not been well developed. This paper examines the theory of complexity and unpacks its epistemological implications for doing interdisciplinary work. The epistemology of complexity demands a cognitive toolkit that includes such things as open-mindedness, tolerance of uncertainty and ambiguity, and intellectual courage. This paper draws out these connections and suggests implications for curriculum development in Interdisciplinary Studies.

Medical School Watercooler Newsletter - August 26, 2022, Lindsey Lyle

Watercooler Newsletter

This is the August 26, 2022 edition of the Frederick P. Whiddon College of Medicine's newsletter - Watercooler.

Contents include:

• Contest supports Student-Run Free Clinic
• Register now: 30th annual OB-GYN conference
• Meet a Med Student: Jerry Andrews
• Faculty, staff and students present at international conference on Alzheimer’s disease
• CCTS Training Symposium set for Sept. 14-16

Vista: August 26, 2022, University Of San Diego

USD Vista (1965-)

No abstract provided.

Chronic Proton-Pump Inhibitor Therapy And Fracture Risk In Women Aged Between 50 And 65 Years: A Retrospective Case-Control Study, Nimesh Patel, Mohamed Fayed, Priyansh Faldu, Wissam Maroun, Janki Chandarana

Anesthesiology Articles

Introduction: Chronic proton-pump inhibitor (PPI) prescription is on the rise in the last decade with an increased prevalence in the elderly population. For most patients, this class of drugs is the primary treatment for various diseases. Even though PPIs are generally safe, long-term use has been associated with multiple adverse effects like bone fractures. The extent of the association between PPI and fracture is still unclear in women aged between 50 and 65 years. Besides, many other variables and risk factors must be accounted for in the analysis of this relation.

Methods: This is a retrospective case-control study looking at …

Generation Z And Media Literacy: Young People’S Perceptions Of Media Literacy Education, Alanna Eavan Powers

Theses - ALL

No abstract provided.

Characterizing Microbiome Variation In Wild Populations Of Mosquito Species, Jane Pascar

Theses - ALL

No abstract provided.

The Marine Gastropod Conomurex Luhuanus (Strombidae) Has High-Resolution Spatial Vision And Eyes With Complex Retinas, Allison R. Irwin, Suzanne T. Williams, Daniel Isaac Speiser, Nicholas W. Roberts

Faculty Publications

All species within the conch snail family Strombidae possess large camera-type eyes that are surprisingly well-developed compared with those found in most other gastropods. Although these eyes are known to be structurally complex, very little research on their visual function has been conducted. Here, we use isoluminant expanding visual stimuli to measure the spatial resolution and contrast sensitivity of a strombid, Conomurex luhuanus. Using these stimuli, we show that this species responds to objects as small as 1.06 deg in its visual field. We also show that C. luhuanus responds to Michelson contrasts of 0.07, a low contrast sensitivity …

Ani314.1 Role Development Example 1, Sae University College

Exemplars

Evidence of output of the student across the Trimester where they provided the following documentation and output:

- Weekly reflection entries in their Learning Journal

- Documented practice within their role specialisation

- Details pertaining to Interdisciplinary practice they engaged in

- Final polished artefact representing their individual Role Specialisation

Whom Do You Want To Be Friends With: An Extroverted Or An Introverted Avatar? Impacts Of The Uncanny Valley Effect And Conversational Cues, Se Jung Kim

Dissertations - ALL

With the rapid growth of social virtual reality platforms, an increasing number of people will be interacting with others as avatars in virtual environments. Therefore, it is essential to develop a better understanding of the factors that could impact initial personality assessments and how they affect the willingness of people to befriend one another. Thin-slice judgment constitutes a quick judgment of a personality based on an avatar, and it could be impacted by the avatar’s appearance, particularly if the avatar elicits an uncanny valley effect that brings negative emotions such as eerieness. However, personality judgments and friendship decisions could also …

Implementing The Mindfulness-Based Kindness Curriculum In An Inclusive Setting: Effects On Prosocial Behavior, Emily Cary

Dissertations - ALL

Low rates of prosocial behavior are associated with poor academic, emotional, and behavioral outcomes, including physical aggression, externalizing problems, criminal justice convictions, lower academic achievement, and more peer rejection. Using a cluster randomized control trial, this study examined the effects of the mindfulness-based Kindness Curriculum (N = 26), compared to a matched control condition consisting of a social and emotional learning (SEL) curriculum, Merrell’s Strong Start (N = 20). Outcome measures included the Strengths and Difficulties Questionnaire and the Head Shoulders Knees and Toes task, which are measures of prosocial behavior including self-regulatory skills. These assessments were administered to 5 …

Redundancy And Developmental Coordination In Myxococcus Xanthus: Insights From The Phenome, Jessica A. Comstock

Dissertations - ALL

Genotype-to-phenotype mapping can typically involve disrupting the function of a gene and observing the impact of mutation on phenotype. While this can be a powerful tool for uncovering gene function, complicating factors such as influences between genes and the environment, epistatic interactions with other genes, and genetic redundancy could all potentially mask the phenotype of a mutation such that functional inferences cannot be made. On the level of the single gene, this may not be particularly informative, but it is possible that studying phenotype in this way at a genome scale might allow for the observation of patterns between genes …

Leveraging Isocyanate Chemistry For Low Cost And Highly Functional Hydrogels, Henry Beaman

Dissertations - ALL

The development of novel biomaterials is an important step in providing higher quality products for wound healing, drug delivery, and tissue engineering. Progress is consistently being made in the development of new polymers, yet new polymer systems are often expensive and/or involve complex multistep synthesis methods. These limitations could hinder the translation and scalability of functionalized materials. To address the issues of complex synthesis and high cost, we have devised strategies for polymeric modification using isocyanate chemistry. Isocyanates are used in the manufacturing of polyurethanes. Isocyanates are highly reactive with common functional groups, such as amines, hydroxyls, and carboxylic acids. …

Investigating The Role Of The Transcriptional Coregulator Cited2 In Regulating Intermediate Progenitor Proliferation And Modulating Behavior, Nikolaus R. Wagner

Dissertations - ALL

The mammalian neocortex develops from a thin layer of neuroepithelial cells into a robust mosaic of differentiated neurons organized into distinct layers and functional areas. This process requires a complex choreography of transcription factors and epigenetic regulators to be spatially and temporally activated in a precise manner. Disruptions in any aspect of this delicate process may lead to neurodevelopmental disorders like autism spectrum disorder, schizophrenia, or intellectual disability. One such transcriptional coregulator that is a crucial regulator of neocortical development is Cited2. Previous work demonstrated that forebrain-specific Cited2 loss-of-function causes a reduction in neocortical progenitor proliferation which leads to reduced …