Digital Commons Network^™

Accelerating Multiparametric Mri For Adaptive Radiotherapy, Shraddha Pandey

USF Tampa Graduate Theses and Dissertations

MR guided Radiotherapy (MRgRT) marks an important paradigm shift in the field of radiotherapy. Superior tissue contrast of MRI offers better visualization of the abnormal lesions, as a result precise radiation dose delivery is possible. In case of online treatment planning, MRgRT offers better control of intratumoral motion and quick adaptation to changes in the gross tumor volume. Nonetheless, the MRgRT process flow does suffer from some challenges that limit its clinical usability. The primary aspects of MRgRT workflow are MRI acquisition, tumor delineation, dose map prediction and administering treatment. It is estimated that the acquisition of MRI takes around …

Social Media, Social Identification, And The Integration Of Refugees Into Jordanian Society, Ibrahim Al Elanat

Student Theses and Dissertations

Mass media and social media platforms play a critical role in helping Syrian refugees integrate into host communities. Through social media platforms, users’ can voice their opinions on various topics related to the refugee experience. This study aims to identify the various cultural needs and practices of the refugee community in Jordan. The study aims to develop a framework for policymakers, employers, and local communities in Jordan to consider and integrate Syrian refugee's social identity. Also this study aims to identify the various aspects of identity re-constructions in order to determine the most effective practices for Syrian refugees. The study …

Enhancing Production Of Functional Proteins In Plant Cell Culture Through Cellular Engineering And Rational Protein Design, Uddhab Karki

Student Theses and Dissertations

Plant cell culture is emerging as a promising alternative bioproduction platform for valuable proteins due to its low cost of production, safety (free from animal pathogen contamination) and ease of scale-up. However, there are common challenges for commercialization, including low protein yields, difficulty in cryopreservation, and non-human glycosylation, etc. This research project addresses the overarching research hypothesis that strategically modifying the plant cell genome using CRISPR/Cas9 technology and rationally designing protein molecules by fusing with a functional module enhance the production of protein in plant cell culture and improve protein function. There are two specific aims addressed in this project: …

The Flaws Of Magic Bullet Theory: Retraining Unconscionability To Discretely Target Different Contexts Of Unfairness In Contracts, Marcus Moore Dr.

Dalhousie Law Journal

Unconscionability has long been a troublesome area in Canadian jurisprudence. This is of significant concern given unconscionability’s pre-eminence as a protection of contractual fairness. This article elaborates a much-needed reorganization and rationalization of unconscionability in Canada. Under current law, a single doctrine hopelessly targets two divergent purposes. I set out here a proposed redevelopment rather of separate common law doctrines, each fit-for-purpose: (1) An English-style unconscionable bargains doctrine for avoiding bargains that exploited disability, and (2) an American-style unconscionable clauses doctrine to control unfair terms in standard form contracts. Extensive Canadian precedent supports this solution, assuring its feasibility and legitimacy. …

Transnational Corporations And Climate Governance: A Case Study Of Amazon.Com’S Net-Zero Climate Pledge, Jason Maclean

Dalhousie Law Journal

“Net zero” has become the predominant way of framing global, national, and nonstate climate change commitments. Hundreds of countries and thousands of corporations promise to achieve net-zero emissions by 2050 or earlier. Hopeful as this may seem, early evidence suggests the need to carefully scrutinize corporations’ climate promises. Specifically, there is an urgent need to critically assess the claim that strategic collaboration and compromise at the science-business-society interface can deliver the transformative social, economic, and political change required to address climate change.

Analyzing Amazon.com’s net-zero pledge as a case study, this article argues that strategic conflict with—and within—transnational corporations is …

Evaluation Of Allogeneic And Autologous Membrane-Bound Il-21-Expanded Nk Cells For Chronic Lymphocytic Leukemia Therapy, Max Yano, Chia Sharpe, J Rachel Lance, Janani Ravikrishnan, Kevan Zapolnik, Xiaokui Mo, Jennifer A Woyach, Deepa Sampath, Adam S Kittai, Sumithira Vasu, Seema Bhat, Kerry A Rogers, Dean A Lee, Natarajan Muthusamy, John C Byrd

Faculty, Staff and Student Publications

Successes with anti-CD20 antibodies in chronic lymphocytic leukemia (CLL) and enhanced activity of Fc-engineered vs unmodified antibody therapy suggest a potentially impactful role for natural killer (NK) cells and other innate immune cells in controlling this disease. Stimulated NK cells have shown promise as a cellular therapy, but their application has been constrained by limited expansion capacity and low cytotoxic activity against CLL cells. Here, we demonstrate that both healthy donor-derived and CLL patient-derived NK cells expand rapidly when stimulated with feeder cells expressing membrane-bound interleukin-21 (mbIL-21) and have potent cytotoxic activity against allogeneic or autologous CLL cells. Combination with …

Electrophilic Aldehyde 4-Hydroxy-2-Nonenal Mediated Signaling And Mitochondrial Dysfunction, Sudha Sharma, Papori Sharma, Tara Bailey, Susmita Bhattarai, Utsab Subedi, Chloe Miller, Hosne Ara, Srivatsan Kidambi, Hong Sun, Manikandan Panchatcharam, Sumitra Miriyala

Department of Chemical and Biomolecular Engineering: Faculty Publications

Reactive oxygen species (ROS), a by-product of aerobic life, are highly reactive molecules with unpaired electrons. The excess of ROS leads to oxidative stress, instigating the peroxidation of polyunsaturated fatty acids (PUFA) in the lipid membrane through a free radical chain reaction and the formation of the most bioactive aldehyde, known as 4-hydroxynonenal (4-HNE). 4-HNE functions as a signaling molecule and toxic product and acts mainly by forming covalent adducts with nucleophilic functional groups in proteins, nucleic acids, and lipids. The mitochondria have been implicated as a site for 4-HNE generation and adduction. Several studies clarified how 4-HNE affects the …

The Link October 25, 2022, Access To Justice Institute

The Link Newsletter

No abstract provided.

Ubr2 Targets Myosin Heavy Chain Iib And Iix For Degradation: Molecular Mechanism Essential For Cancer-Induced Muscle Wasting, Song Gao, Guohua Zhang, Zicheng Zhang, James Z Zhu, Li Li, Yong Zhou, George G Rodney, Reem S Abo-Zahrah, Lindsey Anderson, Jose M Garcia, Yong Tae Kwon, Yi-Ping Li

Faculty, Staff and Student Publications

Cancer cachexia is a lethal metabolic syndrome featuring muscle wasting with preferential loss of fast-twitching muscle mass through an undefined mechanism. Here, we show that cancer induces muscle wasting by selectively degrading myosin heavy chain (MHC) subtypes IIb and IIx through E3 ligase UBR2-mediated ubiquitylation. Induction of MHC loss and atrophy in C2C12 myotubes and mouse tibialis anterior (TA) by murine cancer cells required UBR2 up-regulation by cancer. Genetic gain or loss of UBR2 function inversely altered MHC level and muscle mass in TA of tumor-free mice. UBR2 selectively interacted with and ubiquitylated MHC-IIb and MHC-IIx through its substrate recognition …

The Flaws Of Magic Bullet Theory: Retraining Unconscionability To Discretely Target Different Contexts Of Unfairness In Contracts, Marcus Moore

All Faculty Publications

Unconscionability has long been a troublesome area in Canadian jurisprudence. This is of significant concern given unconscionability’s pre-eminence as a protection of contractual fairness. This article elaborates a much-needed reorganization and rationalization of unconscionability in Canada. Under current law, a single doctrine hopelessly targets two divergent purposes. I set out here a proposed redevelopment rather of separate common law doctrines, each fit-for-purpose: (1) An English-style unconscionable bargains doctrine for avoiding bargains that exploited disability, and (2) an American-style unconscionable clauses doctrine to control unfair terms in standard form contracts. Extensive Canadian precedent supports this solution, assuring its feasibility and legitimacy. …

Novel Chelating Agents For Zirconium-89-Positron Emission Tomography (Pet) Imaging: Synthesis, Dft Calculation, Radiolabeling, And In Vitro And In Vivo Complex Stability, Chi Soo Kang, Shuyuan Zhang, Haixing Wang, Yujie Liu, Siyuan Ren, Yanda Chen, Jingbai Li, Nilantha Bandara, Andrey Yu Rogachev, Buck E Rogers, Hyun-Soon Chong

2020-Current year OA Pubs

We report the synthesis and evaluation of novel chelating agents for zirconium-89 (

‘Meet New Vcd’ Immersive Art Gallery, Wenting Toby Xue

Theses

Students of the Visual Communication Design (VCD) program may have a relatively distressing question about how to explain some details of this program to others. Someone will always ask how to define and explain the VCD. Therefore, in the spirit of redefining the VCD program and creating a platform to more intuitively demonstrate the current success of VCD students in visual design. The curriculum of this program and related student works are mainly user-centered design as the primary starting point. Innovation and forward-looking are necessary. To better communicate the VCD project and expand its influence. According to the questionnaire and …

A Cop1-Gata2 Axis Suppresses Ar Signaling And Prostate Cancer, Tao Shen, Bingning Dong, Yanling Meng, David D Moore, Feng Yang

Faculty, Staff and Students Publications

Androgen receptor (AR) signaling is crucial for driving prostate cancer (PCa), the most diagnosed and the second leading cause of death in male patients with cancer in the United States. Androgen deprivation therapy is initially effective in most instances of AR-positive advanced or metastatic PCa. However, patients inevitably develop lethal castration-resistant PCa (CRPC), which is also resistant to the next-generation AR signaling inhibitors. Most CRPCs maintain AR expression, and blocking AR signaling remains a main therapeutic approach. GATA2 is a pioneer transcription factor emerging as a key therapeutic target for PCa because it promotes AR expression and activation. While directly …

Yap And Taz Promote Osteogenesis And Prevent Chondrogenesis In Neural Crest Cells In Vitro And In Vivo, Xiaolei Zhao, Li Tang, Tram P Le, Bao H Nguyen, Wen Chen, Mingjie Zheng, Hiroyuki Yamaguchi, Brian Dawson, Shuangjie You, Idaliz M Martinez-Traverso, Shannon Erhardt, Jianxin Wang, Min Li, James F Martin, Brendan H Lee, Yoshihiro Komatsu, Jun Wang

Faculty, Staff and Student Publications

Neural crest cells (NCCs) are multipotent stem cells that can differentiate into multiple cell types, including the osteoblasts and chondrocytes, and constitute the majority of the craniofacial skeleton. Here, we show through in vitro and in vivo studies that the transcriptional regulators Yap and Taz have redundant functions as key determinants of the specification and differentiation of NCCs into osteoblasts or chondrocytes. Primary and cultured NCCs deficient in Yap and Taz switched from osteogenesis to chondrogenesis, and NCC-specific deficiency for Yap and Taz resulted in bone loss and ectopic cartilage in mice. Yap bound to the regulatory elements of key …

Human Neutrophil Development And Functionality Are Enabled In A Humanized Mouse Model, Yunjiang Zheng, Esen Sefik, John Astle, Kutay Karatepe, Hasan H Öz, Angel G Solis, Ruaidhrí Jackson, Hongbo R Luo, Emanuela M Bruscia, Stephanie Halene, Liang Shan, Richard A Flavell

2020-Current year OA Pubs

Mice with a functional human immune system serve as an invaluable tool to study the development and function of the human immune system in vivo. A major technological limitation of all current humanized mouse models is the lack of mature and functional human neutrophils in circulation and tissues. To overcome this, we generated a humanized mouse model named MISTRGGR, in which the mouse granulocyte colony-stimulating factor (G-CSF) was replaced with human G-CSF and the mouse G-CSF receptor gene was deleted in existing MISTRG mice. By targeting the G-CSF cytokine-receptor axis, we dramatically improved the reconstitution of mature circulating and tissue-infiltrating …

Waste Management Practices Of Small Hotels In Dehradun, Arakhita Behera, Surendra Singh Jatav

Journal of Tourism Insights

Waste has emerged as a major issue in the modern society. Due to urbanization, the volume of waste is speedily rising. If the origin of waste creation makes no effort, then all wastes are either disposed of or burnt which results in waste of land, air, water, and energy as well as the growth of undesirable animals, plants and microorganism. Through the associate and involvement of the local population, waste may be managed locally in a sustainable manner. Waste management may be an expensive endeavor if not carried out appropriately. In most of the developing nations, 20-50 percent of urban …

'Dreamy Retro Cafe', 'Peaceful Forest Cabin', 'Self Love', & 'Annaliese In The Library', Hannah Gechter

.RAW Journal of Art and Design

No abstract provided.

Full Issue

.RAW Journal of Art and Design

No abstract provided.

Multi‐Scale Effects Of Land Cover, Weather, And Fire On Columbian Sharp‐Tailed Grouse, Bryan S. Stevens, Courtney J. Conway, Jeffrey M. Knetter, Shane B. Roberts, Patrick Donnelly

United States Fish and Wildlife Service: Publications

Columbian sharp‐tailed grouse (Tympanuchus phasianellus columbianus) are endemic to grassland and shrub‐steppe ecosystems of western North America, yet their distribution has contracted to <10% of their historical range. Primary threats to Columbian sharp‐tailed grouse include loss of native habitat and conversion to agriculture, reductions in habitat once provided by the Conservation Reserve Program (CRP), wildfire, and drought conditions, yet population‐level consequences of these threats and their spatio‐temporal scales of effect are poorly understood. We evaluated multi‐scale effects of land cover, weather, and fire histories on patterns of abundance and productivity for Columbian sharp‐tailed grouse populations during 1995–2020 in Idaho, USA, using mixedeffects generalized regression and remotely sensed data. We demonstrated negative effects of fire, tree encroachment, and bare ground, positive effects of spring and summer precipitation and cover of shrubs and perennial forbs and grasses, and positive effects of CRP on grouse abundance that changed in magnitude with cover of perennials and shrubs near leks (i.e., strongest effects when average cover of shrubs and perennial forbs and grasses were less abundant). We also demonstrated per capita recruitment of Columbian sharp‐tailed grouse is positively associated with late‐summer greenness. Our results show that several suspected threats have measurable, population‐level impacts to Columbian sharp‐tailed grouse within Idaho. Moreover, our results suggest ongoing changes occurring within the core range of Columbian sharp‐tailed grouse, including loss of CRP cover to tilled agriculture and changes to wildfire and precipitation dynamics are likely to have negative effects on populations.

The Rider (2022-10-24), Verilu Infante

The Rider Archives

Vol.8 No.9

Florensis: More Catholic Than The Pope?, Gerard Van Der Star, Arjen Van Klink, Florensis

Case Studies

The beauty of a family business is that it always keeps an eye on the continuity of the business. For a family business nothing is more important than long-term continuity and harmony. A good balance between family and business interests is essential24. In any case, the focus is on the long term25. How can Florensis ensure that the fifth and sixth generations and the numerous employees can still enjoy the benefits of this beautiful company. The Calvinist and Christian roots of Florensis ensured from the outset that the human factor was central and key. Without this people orientation quality production …

Simulation Modeling Of A High-Throughput Oyster Cryopreservation Pathway, Sarah Bodenstein, Isabelina Nahmens, Terrence R. Tiersch

Faculty Publications

The genetic resources of oysters in Louisiana and the Gulf of Mexico are threatened due to high unexplained oyster mortality. Germplasm repositories are collections of cryopreserved genetic material stored alongside associated information that are used to protect genetics resources and facilitate breeding programs in agricultural industries. Therefore, there is great need for oyster repositories. Development of repositories has been slow despite research on high-Throughput cryopreservation protocols because of logistical complexities. The goal of this study was to begin to address the gap between cryopreservation research and repository development in oyster aquaculture by modeling a cryopreservation protocol to understand and improve …

Conflict And Nature: How War In Cambodia Shaped Its Natural Landscape, Emily Lifs

Theses and Dissertations

Through the efforts of the Cambodian people and historians, the world is familiar with the tragic human story that unfolded over decades of war and the brutal rule of the Khmer Rouge. This thesis is an attempt to tell the equally tragic but untold story of how decades of conflict shaped the Cambodian landscape and the lives and populations of elephants and other animals.

Vol. 63, No. 09 (October 24, 2022)

Indiana Law Annotated

No abstract provided.

Function Of Bidirectional Sensitivity In The Otolith Organs Established By Transcription Factor Emx2, Young Rae Ji, Yosuke Tona, Talah Wafa, Matthew E. Christman, Edward D. Tourney, Tao Jiang, Sho Ohta, Hui Cheng, Tracy Fitzgerald, Bernd Fritzsch, Sherri M. Jones, Kathleen E. Cullen, Doris K. Wu

Department of Special Education and Communication Disorders: Faculty Publications

Otolith organs of the inner ear are innervated by two parallel afferent projections to the brainstem and cerebellum. These innervations were proposed to segregate across the line of polarity reversal (LPR) within each otolith organ, which divides the organ into two regions of hair cells (HC) with opposite stereociliary orientation. The relationship and functional significance of these anatomical features are not known.Here, we show regional expression of Emx2 in otolith organs, which establishes LPR, mediates the neuronal segregation across LPR and constitutes the bidirectional sensitivity function. Conditional knockout (cKO) of Emx2 in HCs lacks LPR. Tmie cKO, in which mechanotransduction …

Association Between Use Of Remdesivir And Bradycardia, Gibret Umeukeje

USF Tampa Graduate Theses and Dissertations

Remdesivir received the first emergency use authorization from the FDA for the treatment of COVID-19. Multiple adverse drug reactions (ADR) have been reported since its approval in October 2020. Bradycardia, defined by a decrease in heart rate has been reported as an adverse event for patients receiving remdesivir for COVID-19 treatment. The purpose of the research is to systematically investigate the frequency of occurrence of bradycardia in adults receiving remdesivir using clinical data derived from the FDA Adverse Event Reporting System (FAERS) database. Patients receiving remdesivir were compared to those receiving Paxlovid, Regen-Cov, and Dexamethasone for COVID-19 treatment to see …

Development Of Combinatorial Antibody Therapies For Diffuse Large B Cell Lymphoma., Eric S. Geanes, Stacey A. Krepel, Rebecca Mclennan, Stephen Pierce, Santosh Khanal, Todd Bradley

Manuscripts, Articles, Book Chapters and Other Papers

Diffuse large B-cell lymphoma (DLBCL), the most common form of lymphoma, is typically treated with chemotherapy combined with the immunotherapy rituximab, an antibody targeting the B cell receptor, CD20. Despite the success of this treatment regimen, approximately a third of DLBCL patients experience either relapse or have refractory disease that is resistant to rituximab, indicating the need for alternative therapeutic strategies. Here, we identified that CD74 and IL4R are expressed on the cell surface of both CD20 positive and CD20 negative B cell populations. Moreover, genes encoding CD74 and IL4R are expressed in lymphoma biopsies isolated from all stages of …

Addressing Dris (Diagnosis And Recommendation Integrated System) Norms With Potentially Toxic Elements For Assessing The Nutritional Status Of Eucalyptus Amended With Sewage Sludge, Paulo Henrique Silveira Cardoso, Bruno Rodrigues Alves, Beatriz Papa Casagrande, Clayton Alcarde Alvares, Thiago Assis Rodrigues Nogueira, Gian Franco Capra, Arun Dilipkumar Jani, Cassio Hamilton Abreu-Junior

Biology, Agriculture and Chemistry Faculty Publications and Presentations

Background: The Diagnosis and Recommendation Integrated System (DRIS) gave valuable indices of the nutritional status of Eucalyptus amended with sewage sludge (SS).

Aims: Our objective was to establish a DRIS norms and analytical method for Eucalyptus under SS application, by verifying in particular, the influence of potentially toxic elements (PTEs) on the nutritional and plant development.

Method: Data on mean annual increment, nutrient, and PTE concentration were obtained in an experiment at 22, 44, 54, and 76 months after planting Eucalyptus amended with SS.

Results: Our results indicated that DRIS can give valuable data on the nutritional balance indices, in …

The Guardian The Week Of October 24, 2022, Wright State Student Body

The Guardian Student Newspaper

News articles from The Guardian for the week of October 24, 2022. The Guardian is the official student-run newspaper for Wright State University. It has been published regularly since March of 1965.

Re-Connecting Children To Nature In Cities: The Role Of Urban Green Infrastructure Smouha Neighborhood, Alexandria, Egypt Case., Marwa N. Charkas

Mansoura Engineering Journal

Sustainability, by its definition, is an investment in the future. This interprets the importance of linking sustainability studies to children as they are 'the future'. Achieving and practicing sustainability is more than a rigid roadmap to be followed, it is rather beliefs and faiths that have to be deeply rooted and considered truthful. So, embedding environmentally conscious attitudes and right behaviors about nature and its norms is crucial to children. This paper investigates the potential that the green infrastructure could present to reconnect children to the natural environment in cities. It develops a model that correlates all the spatial aspects: …