Digital Commons Network^™

Decoding The Mystery Of Antibiotic Persistence, Tahmina Hossain

Electronic Theses and Dissertations

This work combines microbiology, molecular biology, Next-Generation Sequencing and system biology approaches to explore the mechanism of antibiotic persistence: a multi-drug tolerant, non-dividing, and metabolically altered state present in a subpopulation of cells due to phenotypic diversity rather than genetic variation (i.e. mutations). Persister can survive lethal antibiotic state and resuscitate after the treatment period is over. They are considered as the major contributing factor behind recurring infections. They also have a high mutation rate, which increases the chances of bacteria gaining antibiotic resistance. The formation of this phenotypic variant (persister) threatens the therapeutic effectiveness of antibiotics and understanding how …

High Protein Yeast-Based Ddgs As An Alternative To Commonly Used Protein Sources In Pig Diets, Yesid Ricardo Garavito Duarte

Electronic Theses and Dissertations

Alternative ingredients and co-products from ethanol and biofuel industries represent an opportunity for utilization in swine diets. In this context, understanding nutritional value to practical swine feeding begins with product characterization and determination of nutrient digestibility. The objective of this research was to test the hypothesis: yeast-based high-protein DDGS (HP-GDDY) has comparable AA and metabolizable energy (ME) digestibility to feedstuffs commonly used in swine diets and that it could be included as an alternative protein source in nursery diets without negative impact on growth performance. Cornstarch-based diets were formulated using six ingredients as the sole source of AA: spray dried …

Queer Gothic Literature And Culture, Laura Westengard

Publications and Research

No abstract provided.

Dental Pulp Stem Cell Heterogeneity: Finding Superior Quality “Needles” In A Dental Pulpal “Haystack” For Regenerative Medicine-Based Applications, Zi Y. Kok, Nadia Y.A. Alaidaroos, Amr Alraies, John S. Colombo, Lindsay C. Davies, Rachel J. Waddington, Alastair J. Sloan, Ryan Moseley

Dental Medicine Faculty Research

Human dental pulp stem/stromal cells (hDPSCs) derived from the permanent secondary dentition are recognised to possess certain advantageous traits, which support their potential use as a viable source of mesenchymal stem/stromal cells (MSCs) for regenerative medicine-based applications. However, the well-established heterogeneous nature of hDPSC subpopulations, coupled with their limited numbers within dental pulp tissues, has impeded our understanding of hDPSC biology and the translation of sufficient quantities of these cells from laboratory research, through successful therapy development and clinical applications. This article reviews our current understanding of hDPSC biology and the evidence underpinning the molecular basis of their heterogeneity, which …

Improving Active Learning Through Advanced Teaching Practices On An Online Course, Jin Ouk O. Choi

UNLV Best Teaching Practices Expo

Since the outbreak of the COVID, there has been a great need to advance learning experiences and opportunities for students when the courses are taught remotely. To address the need, the author incorporated the teaching strategies learned from the Culturally Relevant & Responsive Teaching (CRRT) fellows program supported by the NSF TRANSCEnD project. In Fall 2021, the author implemented multiple new teaching techniques and strategies in the CEM453-653 course, delivered entirely online. The specific teaching practices and technologies are: 1) Delivering lectures both live (synchronous) and recordings (asynchronous); 2) Using adaptive technology (Mentimeter & WebEx Poll) with real-time feedback; 3) …

High Resistant Starch Rice: Variation In Starch Related Snps, And Functional, And Sensory Properties, Ming Hsuan Chen, Karen Bett-Garber, Jeanne Lea, Anna Mcclung, Christine Bergman

Hospitality Faculty Research

Human diets containing greater resistant starch (RS) are associated with superior glycemic control. Although high amylose rice has higher RS (29 g/kg to 44 g/kg) than lower amylose content varieties, sensory and processing properties associated with RS have not been evaluated. This study used variants of Waxy and starch synthase II a (SSIIa) genes to divide high amylose (256 g/kg to 284 g/kg) varieties into three haplotypes to examine their effects on RS, RVA parameters, and 14 cooked rice texture properties. RVA characteristics were influenced by both genes with peak and hotpaste viscosity differentiating the three haplotypes. Setback from hotpaste …

Common Features In Compulsive Sexual Behavior, Substance Use Disorders, Personality, Temperament And Attachment—A Narrative Review, Yaniv Efrati, Shane W. Kraus, Gal Kaplan

Psychology Faculty Research

Do addictions share common traits of an “addictive personality” or do different addictions have distinct personality profiles? This narrative review examines the differences in the associations between substance use disorder (SUD) and compulsive sexual behavior disorder (CSBD), on the one hand, and personality traits, attachment dispositions, and temperament, on the other hand. We found that both people with a SUD and people with CSBD tended to be more spontaneous, careless, and less reliable, to place self-interest above getting along with others, to show emotional instability and experience negative emotions such as anger, anxiety, and/or depression, to be less able to …

Smartphones And Learning: An Extension Of M-Learning Or A Distinct Area Of Inquiry, Kendall Hartley, Alberto Andújar

Teaching and Learning Faculty Research

The smartphone has become an integral part of the education landscape. While there has been significant smartphone research in education under the guise of m-learning, the unique role of the device suggests that m-learning may not be an appropriate characterization. The purpose of this paper is to review the use of m-learning as a primary descriptor for smartphone-and learning-related research. In support of this goal, the paper reviews the definitions associated with m-learning, smartphones, and related technologies from the perspective of educational research. In addition, a review of author keywords of research on smartphones in education is used to provide …

A Balancing Act: Analyzing The Effects Of Embedded Versus Isolated Self-Regulation Breathing Techniques On Inattention And Phonemic Segmentation Skill Acquisition In Kindergartners, Kimberly A. Atkinson

Doctor of Education (Ed.D.)

Using a mixed-methods convergent design within an Improvement Science framework, this Improvement Science Dissertation in Practice analyzed the effects of embedding self-regulation breathing techniques into the phonemic segmentation lesson versus conducting the techniques prior to the lesson or not at all. The project was conducted across two elementary schools, using six kindergarten educators and classes. The research began with a pre-assessment, followed by two days of applying or withholding the intervention across three groups (no intervention, isolated intervention, embedded intervention), and concluded with a post-assessment. The researcher conducted the academic lessons while the educators conducted inattention behavioral observations. After each …

Documenting The Rapidly Expanding Distribution Of Invasive Ravenna Grass (Tripidium Ravennae) Eastern In Kansas, Rachel A. Styers

Electronic Theses & Dissertations

Invasive species cause significant ecological losses in the United States where they cost approximately $21 billion dollars a year to manage (Fantle-Lepczyk et al., 2022). Early detection of new invasive species, coupled with a rapid response of management efforts, can help to slow the ecological and economic impacts caused by these habitat invaders. Tripidium ravennae (L.) H. Scholz (or Ravenna grass) is a tall, robust, cespitose grass known to occur in Kansas, although its distribution remained poorly documented prior to this study. Given its known invasive tendencies, it has been declared noxious in at least six US states. Coupled with …

A Modified Spectroscopic Approach For The Real-Time Detection Of Pollen And Fungal Spores At A Semi-Urban Site Using The Wibs-4+, Part I, Emma Markey, Jerry Hourihane Clancy, Moisés Martínez-Bracero, Finnian Neeson, Roland Sarda-Estève, Dominique Baisnée, Eoin Mcgillicuddy, Gavin Sewell, David J. O’Connor

Articles

The real-time monitoring of primary biological aerosol particles (PBAP) such as pollen and fungal spores has receivedmuch attention in recent years as a result of their health and climatic effects. In this study, theWideband Integrated Bioaerosol Sensor (WIBS) 4+ model was evaluated for its ability to sample and detect ambient fungal spore and pollen concentrations, compared to the traditional Hirst volumetric method. Although the determination of total pollen and fungal spore ambient concentrations are of interest, the selective detection of individual pollen/fungal spore types are often of greater allergenic/agricultural concern. To aid in this endeavour, modifications were made to the …

Review Of Mathematical Modelling Techniques With Applicationsin Biosciences, Ahmed Hasan Alridha, Ahmed Sabah Al-Jilawi, Fouad H. Abd Alsharify

Iraqi Journal for Computer Science and Mathematics

Modelling can provide intellectual frameworks that are necessary to translate data into knowledge.Mathematical modelling has played an important role in many applications, such as ecology, genetics, engineering,psychology, sociology, physics and computer science, in recent years. This study focused on reviewing mathematicalmodelling and its applications to biological systems by tracing many metabolic activities of cellular interactions onthe one hand and between the spread of epidemics and population growth on the other hand. Various mathematicalequations have played fundamental roles in the formation of these systems for model development proceduresby describing them mathematically and establishing relationships that characterise the dynamics of a biologicalphenomenon. …

Effects Of Fractional Gravity On Human Gait, Elizabeth Nicole Smith

Theses and Dissertations

Only twelve humans have stepped on the Moon for a mere 80 hours. Further, noneof that Lunar exposure has been within the last 50 years. NASA’s Artemis Program recently announced that the next manned missions to the Moon will be scheduled for 2024 and 2025. With only a few years to prepare, it is pertinent that training for prospective astronauts reflects current scientific knowledge to ensure safety. In this study, the coordinative gait structures under 1.0g (Earth), as well as simulated 0.38g (Martian) and 0.17g (Lunar), were analyzed to discover critical changes in gait that may occur in these environments. …

Meta-Analysis For Comparing Effect Between Game-Based Learning Versus Gamification Utilized In Nutrition Education, Chia-Lin Chang

Theses and Dissertations

The prevalence of obesity tripled from 1975 to 2016 and was declared as a global epidemic by the World Health Organization (WHO) in 1997 (Haththotuwa et al., 2020; "Controlling the global obesity epidemic," 2022). Nutrition education that involves knowledge and behavioral change is one major component addressing the problem ("Controlling the global obesity epidemic," 2022). Therefore, the effectiveness of each learning session is essential (Sharifirad et al., 2013). Thus, many nutrition educators have used gamified nutrition education to improve teaching effectiveness to increase healthy behavior or knowledge (Chow et al., 2020.; Munguba et al., 2008; Azevedo et al., 2019). Two …

Evaluating Virtual Reality And Artificial Intelligence As Solutions For Delayed Flight Progress In Aviation Pilot Training, Ryan Paul Guthridge

Theses and Dissertations

Three studies in this dissertation examined a topic centered around delayed flight progress in aviation pilot training. Study one explored the impact of nonconcurrent flight laboratory training on the academic outcomes of collegiate aviation students, while studies two and three explored virtual reality and artificial intelligence as potential solutions to help alleviate the strain of delayed flight progress on the flight training organization. In the first study (n = 144), it was found that concurrent enrollment in an aviation classroom ground course and flight training laboratory positively impacts the mean academic block exam scores of students. In study two (n …

A Machine Learning Approach To Intended Motion Prediction For Upper Extremity Exoskeletons, Justin Berdell

Graduate Research Theses & Dissertations

A fully solid-state, software-defined, one-handed, handle-type control device built around a machine-learning (ML) model that provides intuitive and simultaneous control in position and orientation each in a full three degrees-of-freedom (DOF) is proposed in this paper. The device, referred to as the “Smart Handle”, and it is compact, lightweight, and only reliant on low-cost and readily available sensors and materials for construction. Mobility chairs for persons with motor difficulties could make use of a control device that can learn to recognize arbitrary inputs as control commands. Upper-extremity exoskeletons used in occupational settings and rehabilitation require a natural control device like …

Gas Phase C-H And C-C Bond Activation By Ternary Metal Complexes, Kevin Parker

Graduate Research Theses & Dissertations

The activation of relatively inert C-H bonds has remained a challenge in organic chemistry for over a century. Metal catalysts have been deployed to overcome the labile reactivity of these bonds and through gas-phase studies, detailed mechanisms can be elucidated for this important class of chemistry. The powerful combination of ion-molecule reactions, collisional activation of ions, and density functional theory calculations allows for structural elucidation, intermediate determination, and as shown throughout this work, a complete picture of catalytic processes. Modified linear ion-trap mass spectrometers were used to trap and generate reactive intermediates of the form [(L)(M)(X)]+ (L= auxiliary ligand; M= …

Impacts Of Plastic Pollution On A Pelagic Marine Mammal, The Northern Elephant Seal, Lauren M. Kashiwabara

University of the Pacific Theses and Dissertations

As plastic pollution increases, top marine predators such as marine mammals are becoming increasingly susceptible to plastic particles and their additives. Plastic particles have been found in gastrointestinal tracts and scat of many marine mammals, and quantifying plastic pollution in those that are pelagic can provide insight into plastic pollution in mesopelagic ecosystems that are just beginning to be analyzed. Adapting well-developed laboratory techniques for microplastic (MP) isolation (i.e. density separation and chemical digestion), I isolated MPs from the scat of the deepest diving pinniped, the northern elephant seal (NES), and found that100% of scat samples (n=11) contained high counts …

Incorporating Glutamic Acid-Valine-Citrulline Linker In Trifunctional Molecules Targeting Psma Ensures Enhanced Stability, Safety, And Efficacy In Mouse Model Of Prostate Cancer, Toufiq Ul Amin

University of the Pacific Theses and Dissertations

This project describes the development of a new antitumor therapeutic platform that combines the benefits of small-molecule drug conjugates (SMDCs) and antibody drug conjugates (ADCs). Valine-citrulline (VCit) dipeptide linkers are popular cathepsin B cleavable ADC linkers. Due to its instability in mouse serum, translating efficacy data from mouse to human is more difficult. It has been reported that replacing the VCit linker with glutamic acid-valine-citrulline (EVCit) improves ADC stability in mouse serum. The effect of the EVCit linker on the stability of SMDCs has not been reported so far. In a xenograft mouse model of prostate cancer, we found that …

Tourism Trails And Resurgent Pathways: An Exploration Of The Nexus Between Indigenous-Led Tourism And Resurgence Discourses Alongside Karajarri Traditional Owners Of The Saltwater Kimberley, Australia, And Beyond, Bobbie Chew Bigby

Theses

This research explores the intersections between Indigenous-led tourism and resurgence among Indigenous communities. Resurgence is understood as practices promoting connections with culture, Country (i.e. Indigenous lands and waterways), and community. The research utilises a comparative case study approach to specifically analyse the experience of Karajarri Traditional Owners (TOs) of the saltwater Kimberley region of northwestern Australia. Comparative examples are drawn from Indigenous-led tourism development by other coastal Kimberley Aboriginal tourism leaders, as well as from the author’s own Tribal Nation, the Cherokee Nation of Oklahoma, United States.

The conceptual framework for this work is derived from the following concepts from …

Does Acupuncture/Dry Needling Improve Pain And Itch In Abnormal Hypertrophic Scars, Catherine Tuckey

Theses

This research program investigated the use of acupuncture intervention as an adjunct for hypertrophic scar (HTS) management. Mechanistic evidence from ex vivo and animal studies suggests that acupuncture has the potential to modulate neurogenic inflammation to influence pain and itch associated with HTS. Further, acupuncture may mediate non-neuronal cells involved in the proliferation and remodelling stage of healing to promote scar maturation. Previous studies using acupuncture for scar management demonstrated positive outcomes, however low-quality clinical trials and case studies provide limited evidence for treatment benefit.

A treatment protocol was designed to investigate the effects of locally applied acupuncture compared to …

Community Development Supporting Ecological Conversion, As Identified In Laudato Si’: On Care For Our Common Home, Anne Jennings

Theses

In this extraordinary time of multiple, intersecting crises with compounded costs in social, economic, and environmental degradation, calls for transformative change are emerging from all levels of humanity – global to local. Within that setting, my study concentrated on investigating change at a local level to address the research question ’How can community development theory and practice contribute to ecological conversion, as identified in Laudato Si’: On Care for Our Common Home?’

Since its release, Laudato Si’ has provoked considerable academic/theological and scientific/environmental debate that does not necessarily contain local people’s views and everyday lived experiences, given their often-limited access …

Reinterpreting The Villain: Changing The Pattern Of The Fairy Tale Through Sleeping Beauty’S Maleficent, Mackenzie Waldon

Theses

Recent filmic representations of the fairy tale villain can offer powerful counter-narratives to archetypal fairy tale stories. This thesis examines how certain contemporary representations of fairy tales have reinterpreted the traditional ‘villain’ archetype, revealing a greater diversity of perspectives. Using John Stephens’ and Peter Stockwell’s cognitive poetics theory on schema and scripts as a theoretical framework, this thesis closely analyses the known Disney fairy tale villain, Sleeping Beauty’s (1959) Maleficent, as she is portrayed in the live-action films Maleficent (2014) and Maleficent: Mistress of Evil (2019), as well as Disney’s Descendants (2015, 2017, 2019) films. By analysing the patterns and …

The Birds, The Bees, The Trees, And The Breeze: Expanding The Moral Universe Throught Rsk Anlysis, Jared Gibbs

Graduate Student Theses, Dissertations, & Professional Papers

No abstract provided.

Preliminary Study In More Effective Utilization Of 87sr/86sr Analysis In The Field Of Forensic Anthropology, Samantha Powers

Graduate Student Theses, Dissertations, & Professional Papers

Isotopic analyses are gaining attention in the field of anthropology. The current utilization of strontium isotopes primarily focuses on residence and migration patterns of past populations extracting data from tooth enamel to indicate early-life residence This study aims to identify a skeletal feature to be set as a field standard in strontium analysis of modern remains when seeking end-of-life residence, the impact of diagenesis on modern remains, and to test the accuracy of the petrous as an alternative to tooth enamel.

The results of this research were unable to suggest such a standard but gathered helpful data to be used …

Challenges And Promises Of The Agricultural Sector In The West Today, Jordan J. Unger

Graduate Student Theses, Dissertations, & Professional Papers

The transition from hunter-gatherers to a sedentary, agrarian based society was a pivotal point in history, fundamentally altering humanity’s trajectory. Early human’s domestication of plants and animals paved the way for modern civilization and drastically changed the way we lived and interacted. With agriculture, humans could invest more time into other activities besides procuring food while simultaneously being able to support increasingly large communities of people. Villages, city-states and, ultimately, states were formed – helping give rise to more complex social structures.

Until recently, agriculture has been the main economic backbone of every civilization. In 1970 in the United States, …

Other Orchards, Sam B. Robison

Graduate Student Theses, Dissertations, & Professional Papers

Other Orchards is comprised of poems each suspect in their own way of those boundaries that might separate humans from nature, rural from urban, worker from scholar, or human from beast. Using the figure of the orchard, a kind of “false forest,” this collection studies the ways we map ourselves onto our work and the way work might inform an understanding of the self. Ultimately, these are poems that emerge from the seams of things—the shoulder of highway strewn with dead antelope, the feral apple tree lost to the woods, the farmer lost in their work, slowing becoming less and …

Conserving And Sharing Freshwater In The West, Kelsea Harris-Capuano

Graduate Student Theses, Dissertations, & Professional Papers

There are infinite stories to be told about water in the West. As an essential and finite resource, countries, states, tribal nations and neighbors must figure out how to manage this shared resource, whether for ecosystem sustainability, agricultural, household, or recreational use.

The Flathead Lake Biological Station in Polson, MT is one of the oldest active biological research stations in the United States. One of its former faculty, Dr. Mark Lorang, has been working on Flathead Lake’s erosion problem for over 30 years. As a result of seasonal lake level fluctuations controlled by the dam, erosion over the years has …

Rancher Perspectives On Livelihood Diversification Options In Montana's Blackfoot River Watershed, Sara N. Schmidt

Graduate Student Theses, Dissertations, & Professional Papers

The Blackfoot River watershed in Western Montana, like many rural landscapes across the Intermountain West, has faced a suite of economic, demographic, and social change over the last 40 years. Cattle ranching specifically, a principal land use in the Blackfoot watershed, has faced increasing socioeconomic challenges due to global competition, falling cattle prices, amenity migration, and climate change. Ranch livelihood diversification presents an opportunity to bolster the profitability of ranches in the watershed, while also supporting the well-being of rural communities and conserving the intact, ecologically-significant rangelands upon which ranching depends. The Blackfoot Challenge (BC) is a nonprofit community-based conservation …

The Mountain Home: Spatial And Optimal Foraging Assessments Of Hunter- Gatherer Mountain Landscape Use In The Beartooth Mountains, Montana, Scott William Dersam

Graduate Student Theses, Dissertations, & Professional Papers

Patterns of cultural mountain adaptations are recorded throughout the Western Cordillera of North America. They demonstrate prolonged and complex uses of these ecosystems over the Holocene. What behaviors or circumstances in the Early Holocene transitioned mountains from inhospitable to reliable territory? This research addresses this question in the Beartooth Mountains of the Greater Yellowstone Ecosystem (GYE).

Foraging behaviors dictate hunter-gatherer mobility and habitation patterning. These behaviors are reflected in the spatial arrangement of archaeological activity areas and lithic tools in mountainous ecosystems. Energy-demanding ecosystems, require foraging behaviors and habitation strategies that conserve energy. I assert that to understand the emergence …