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2021

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Predictors Of Time To Hospital Among People With Colorectal Cancer, Saimai Tumwijit Jan 2021

Predictors Of Time To Hospital Among People With Colorectal Cancer, Saimai Tumwijit

Chulalongkorn University Theses and Dissertations (Chula ETD)

This retrospective, cross-sectional study aims to describe time to hospital, relating factors, and determine predictors of time to hospital among people with colorectal cancer. Multistage random sampling was used to yield a probability of 240 people with colorectal cancer visiting medical and surgical outpatient departments, oncology units, radiology units, endoscopic units, and inpatient departments. Research instruments consisted of personal information sheet, time to colorectal cancer diagnosis questionnaire, knowledge about colorectal cancer questionnaire (KR-20 = .786), The modified illness perception questionnaire-revised (a = .674 - .836), health care seeking behavior questionnaire (a = .706), and perceived seriousness of warning signs and …

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Effect Of Different Placement Methods On Voids Formationin Class Ii Cavity Restored With Bulk-Fill Resin Composite, Parinthorn Siyoputhawong Jan 2021

Effect Of Different Placement Methods On Voids Formationin Class Ii Cavity Restored With Bulk-Fill Resin Composite, Parinthorn Siyoputhawong

Chulalongkorn University Theses and Dissertations (Chula ETD)

The aim of this study was to evaluate void formation by micro-computed tomography in two-surface Class II cavities restored with two bulk-fill resin composites using different placement methods. Standardized Class II cavities were prepared in forty intact human maxillary first premolar teeth. The teeth were randomly divided into four groups and restored with (n=10): 1) one bulk placement with hand instrument; 2) two-bulk placement with hand instrument; 3) one bulk placement with injectable dispenser; 4) one bulk placement with injectable dispenser and sonic-activated handpiece. Percentage of void formation in the entire restoration was evaluated. Welch's ANOVA and Games-Howell post hoc …

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Factors Influencing The Dentist’S Decision To Propose A Tooth Autotransplantation In The Faculty Of Dentistry, Chulalongkorn University, Thunchanok Sinprasurdsook Jan 2021

Factors Influencing The Dentist’S Decision To Propose A Tooth Autotransplantation In The Faculty Of Dentistry, Chulalongkorn University, Thunchanok Sinprasurdsook

Chulalongkorn University Theses and Dissertations (Chula ETD)

Objectives: To study factors which influence a dentist’s decision to propose the Tooth Autotransplantation (AT). Material and methods: A cross-sectional study was conducted among 99 dentists between January and March 2021. A questionnaire comprised demographic characteristics, unprimed scenario, primed scenario, reasoning behind decisions, experience, and knowledge of AT. Data were analyzed using the Chi-square test, McNemar’s test, and multiple logistic regression. Results: The respondents comprised 73 females and 26 males with a mean age of 30.84 ± 6.238 years. In the unprimed scenario, there were significant associations between fields of expertise, experience, knowledge of current indications, outcomes, and the benefits …

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Role Of Extracellular Adenosine Triphosphate (Eatp) On Immunomodulatory Function Of Human Periodontal Ligament Cells, Maythwe Kyawsoewin Jan 2021

Role Of Extracellular Adenosine Triphosphate (Eatp) On Immunomodulatory Function Of Human Periodontal Ligament Cells, Maythwe Kyawsoewin

Chulalongkorn University Theses and Dissertations (Chula ETD)

Background: Adenosine triphosphate, a nucleotide that acts as an important extracellular messenger, is released into the extracellular environment during various physiologic and pathological conditions. Human periodontal ligament cells (hPDLCs) can release adenosine triphosphate into the extracellular environment in response to mechanical stress. Extracellular adenosine triphosphate (eATP) plays role in both inflammation and osteogenic differentiation processes. eATP also participates in immunosuppressive action on immune cells. However, the role of eATP on the immunomodulatory function of hPDLCs is still unclear. This study aims to investigate the effects of eATP on the immunomodulatory function of hPDLCs and the participation of specific purinergic P2 …

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Anti-Inflammatory And Regeneration Properties Of Iloprost On Inflamed Human Dental Pulp Cells Model: An In Vitro Study, Rahman Wahyudi Jan 2021

Anti-Inflammatory And Regeneration Properties Of Iloprost On Inflamed Human Dental Pulp Cells Model: An In Vitro Study, Rahman Wahyudi

Chulalongkorn University Theses and Dissertations (Chula ETD)

In the clinical situation, pulp exposure can happen during caries removal procedures. Inflammation can occur on the pulp of the exposed area because of infiltration of bacteria from caries or as a result of pulp reaction to caries removal drilling. When the pulp was exposed during the caries removal procedure, traditional methods to preserve the pulp such as pulp capping and pulpotomy were performed. The ideal treatment outcome of the pulp exposure is to regain the primary structure of tubular dentin as well as maintain the vitality and healthiness of the dental pulp Thus, good dental materials or drugs used …

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Effect Of Shear Force On Immunosuppressive Property Of Human Periodontal Ligament Cells, Ravipha Suwittayarak Jan 2021

Effect Of Shear Force On Immunosuppressive Property Of Human Periodontal Ligament Cells, Ravipha Suwittayarak

Chulalongkorn University Theses and Dissertations (Chula ETD)

The immunomodulatory property is to regulate immune cell function. IDO and TGF-β1 inhibited T cell proliferation and induced regulatory T (Treg) cells differentiation. However, this property must be activated by inflammation. Recently, mechanical forces have also activated this property. Hence, this study investigated effect of shear stress on immunosuppressive property of hPDL cells. Cells were subjected to shear stress then expression of IDO, TGF-β1, IFN-gamma and COX2 mRNA and protein was examined by qRT-PCR, ELISA, and IDO activity. Conditioned media derived shear stress were treated with CD4+ T cells. The proliferative T cell effect was determined using resazurin. The FOXP3, …

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Efficacy Of Cannabidiol (Cbd) For The Treatment Of Recurrent Aphthous Ulcers, Chalapinyo Umpreecha Jan 2021

Efficacy Of Cannabidiol (Cbd) For The Treatment Of Recurrent Aphthous Ulcers, Chalapinyo Umpreecha

Chulalongkorn University Theses and Dissertations (Chula ETD)

The objectives of this research were 1) To investigate the allergic reactions to cannabidiol (CBD) when used on human skin. 2) To assess local and systemic side effects of CBD when used on normal oral mucosa. 3) To compare the efficacy among 0.1% CBD, 0.1% triamcinolone acetonide (TA), and placebo (pure oral paste) in recurrent aphthous ulcer (RAU) treatment. This project was composed of 3 phases. Phase 1, a skin patch test was performed on 100 healthy subjects. Phase 2, CBD was applied to normal oral mucosa of 50 healthy subjects 3 times/day for 7 days. Oral examination, vital signs, …

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Effects Of Local Versus Systemic Administration Of Cxcr4 Inhibitor Amd3100 On Orthodontic Tooth Movement In Rats, Narubhorn Ongprakobkul Jan 2021

Effects Of Local Versus Systemic Administration Of Cxcr4 Inhibitor Amd3100 On Orthodontic Tooth Movement In Rats, Narubhorn Ongprakobkul

Chulalongkorn University Theses and Dissertations (Chula ETD)

Chemokines play pivotal roles in orthodontic tooth movement (OTM) through osteoclast-mediated bone resorption, but the underlying mechanism remains unclear. We aimed to elucidate the effects of serial local vs systemic administration of the chemokine receptor CXCR4 antagonist AMD3100 on OTM. The maxillary right first molar (M1) of rats was moved mesially using a 10 g of force nickel-titanium coil spring. The injections were performed every other day with phosphate-buffered saline as a control, whereas local and systemic animals were injected with AMD3100 at the buccal palatal mucosa adjacent to M1 and subcutaneously, respectively. OTM distance and alveolar bone were examined …

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Differences Of Sound And Morphology In Skeletal Class Iii Patients, Natthaporn Pravitharangul Jan 2021

Differences Of Sound And Morphology In Skeletal Class Iii Patients, Natthaporn Pravitharangul

Chulalongkorn University Theses and Dissertations (Chula ETD)

[Background] This study aimed to explore differences in vowel production using acoustic analysis in skeletal Class III and Class I Japanese participants, and to investigate the correlation between vowel sounds and cephalometric variables in skeletal Class III subjects. [Materials and Methods] Japanese males with skeletal Class III (ANB<0°) and Class I skeletal relationships (0.62°

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The Designs Of Miniscrew-Assisted Transpalatal Arch Affect Molar Intrusion, Ornnicha Pooktuantong Jan 2021

The Designs Of Miniscrew-Assisted Transpalatal Arch Affect Molar Intrusion, Ornnicha Pooktuantong

Chulalongkorn University Theses and Dissertations (Chula ETD)

Abstract: An anterior open bite is one of the most difficult malocclusions in orthodontic treatment. For such malocclusion, orthodontic miniscrews insertion into both buccal and palatal alveolar regions has been indicated for molar intrusion, but it involves a risk of tooth root injury. To solve the problem, midpalatal miniscrew-attached extension arm (MMEA) is adopted. However, this method causes palatal tipping of the molar because intrusive loads were applied only from palatal side. Currently, transpalatal arch is added to avoid tipping movement, but it induces patient's discomfort. Hence, the objective of this study was to evaluate the loading conditions for maxillary …

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Effects Of Intrauterine Intermittent Hypoxia On Skeletal Muscle In Offspring Rats, Wirongrong Wongkitikamjorn Jan 2021

Effects Of Intrauterine Intermittent Hypoxia On Skeletal Muscle In Offspring Rats, Wirongrong Wongkitikamjorn

Chulalongkorn University Theses and Dissertations (Chula ETD)

Gestational intermittent hypoxia (IH), a hallmark of OSA, alters the offspring's respiratory neural control and diaphragm contractile function. Thus, we aimed to investigate the effects of gestational IH on the muscle development and metabolism of geniohyoid (GH), which is innervated by the respiratory-related hypoglossal nerve and plays a role in tongue traction and suckling, in male offspring rats compared with masseter (MAS), the largest masticatory muscle. Pregnant Sprague-Dawley rats were exposed to IH (3-min periods of 4–21% O2) compared to Normoxia for 8 hours/day during gestational days 7–20. GH and MAS from 35-day-old male offspring (n = 6 /group) were …

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Cellular Responses Of Stem Cells Isolated From Human Exfoliated Deciduous Teeth Towards Different Concentration Of Calcium Ion, Thanika Phlinyos Jan 2021

Cellular Responses Of Stem Cells Isolated From Human Exfoliated Deciduous Teeth Towards Different Concentration Of Calcium Ion, Thanika Phlinyos

Chulalongkorn University Theses and Dissertations (Chula ETD)

Objectives: The purpose of this study was to investigate the effect of Ca2+ on proliferation, osteogenic differentiation, and migration of stem cells from human exfoliated teeth (SHEDs) in vitro Materials and methods: SHEDs were seeded in culture media and osteogenic induction media containing 1.8-16.2 mM of Ca2+. SHEDs proliferation was determined using MTT assay and colony forming unit assay. Osteogenic differentiation was evaluated using mineralization assay and osteogenic marker gene expression and cell migration was evaluated using wound healing assay. Values were expressed as mean + S.D. Statistical analysis of MTT assay and wound healing assay were performed using two-way …

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Expression Of Vascular Endothelial Growth Factor Protein In Mrna-Transfected Human Periodontal Ligament Cells, Mana Naratippakorn Jan 2021

Expression Of Vascular Endothelial Growth Factor Protein In Mrna-Transfected Human Periodontal Ligament Cells, Mana Naratippakorn

Chulalongkorn University Theses and Dissertations (Chula ETD)

The complete regeneration of periodontal tissues following current periodontal therapy remain challenging and unpredictable. Nucleoside-modified messenger RNA (mRNA) technology can be a promising novel platform in regenerative medicine. The aims of this study were to evaluate whether pseudouridine modified mRNA encoding vascular endothelial growth factor (VEGF) could induce VEGF production in human periodontal ligament cells (PDLCs) and this translated protein function by promoting in vivo blood vessel formation using chorioallantoic membrane (CAM) assay. Isolated PDLCs from healthy periodontal tissue were transfected with modified mRNA encoding VEGF (VEGF mRNA) complexed with a transfecting agent, Lipofectamine 2000 (L2000) and L2000 alone (control). …

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Sustainable Two-Step Minlp Heat Exchanger Network Synthesis With Practical Detailed Design, Thanawat Boonvaerojkul Jan 2021

Sustainable Two-Step Minlp Heat Exchanger Network Synthesis With Practical Detailed Design, Thanawat Boonvaerojkul

Chulalongkorn University Theses and Dissertations (Chula ETD)

Due to sustainable development that focuses on energy conservation, decreasing emissions and environmental effect, the research of heat exchanger network synthesis (HENS) is still necessary and challenging. Most academic studies in the subject of HENS aim on reducing total annual cost (TAC) through optimal topology design without the detailed design costs of each heat exchanger in the network, resulting in conceptual HEN designs. Hence, the detailed design of shell and tube heat exchangers is recalculated for TAC and corrected area costs, including the pumping costs from overall pressure drop of heat exchangers. For a shell and tube heat exchanger, its …

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Production Of Bio-Olefins From Oleic Acidvia Oxidative Dehydrogenation Using Vanadium Oxides/Sba-15 Catalysts, Nattaporn Chaidherasuwet Jan 2021

Production Of Bio-Olefins From Oleic Acidvia Oxidative Dehydrogenation Using Vanadium Oxides/Sba-15 Catalysts, Nattaporn Chaidherasuwet

Chulalongkorn University Theses and Dissertations (Chula ETD)

Long-chain olefins (≥C10) are important commodity chemicals, which are primarily used to manufacture everyday products such as plastic packaging, surfactants and detergents in cleaning products, and lubricant oil additives. However, the long-chain olefins are currently synthesized from the petroleum refining process via ethylene oligomerization or thermal cracking of long-chain paraffins. To support the Bio-Circular-Green Economy (BCG) model, the direct synthesis of olefins from renewable feedstocks has become an attractive route to promote overall sustainability. The objective of this research was to study the possibility to transform oleic acid (OA), one of the unsaturated fatty acids in palm oil, to produce …

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Determination Of Adsorption And Separation Of Co2/N2 And H2s/Ch4 Mixtures In Porous Materials By Molecular Simulation, Tanawut Ploymeerusmee Jan 2021

Determination Of Adsorption And Separation Of Co2/N2 And H2s/Ch4 Mixtures In Porous Materials By Molecular Simulation, Tanawut Ploymeerusmee

Chulalongkorn University Theses and Dissertations (Chula ETD)

The major resources of power plant in the world rely on coal and natural gas which are economical for generating electricity. Flue gases, mainly N2 and CO2, are produced and released into the atmosphere. The zeolitic imidazolate framework-90 (ZIF-90) obtains a high potential as being a candidate for several applications. Consequently, the basic knowledge of adsorption and diffusion of gases in this material is the key factor of improving this gas separation technique. The effect of adsorbed CO2 molecules on the lattice structure of ZIF-90 can lead to gate opening depending on the amount of adsorbed guest molecules and the …

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Clinical Efficacy Of Silk Sericin Dressing With Collagen For Split-Thickness Skin Graft Donor Site Treatment, Apirujee Punjataewakupt Jan 2021

Clinical Efficacy Of Silk Sericin Dressing With Collagen For Split-Thickness Skin Graft Donor Site Treatment, Apirujee Punjataewakupt

Chulalongkorn University Theses and Dissertations (Chula ETD)

Sericin dressing containing collagen hydrolysate (SDC) was evaluated in this study, consisting of two phases of clinical trials. The clinical safety of SDC was determined using a patch test in 103 healthy volunteers (phase I). Most of the results (around 90%) in the SDC group were interpreted as negative and doubtful reactions, and the remaining results (around 10%) were weak reactions. After that, the clinical efficacy and safety of SDC were investigated in 21 patients with 30 split-thickness skin graft (STSG) donor sites (phase II). Each donor site was divided into two equal sites. SDC or the commercial dressing (Bactigras®) …

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Production Optimization Of Porcine Reproductive And Respiratory Syndrome (Prrs) Virus Nucleocapsid Protein In Nicotiana Benthamiana, Boonyasit Porngarm Jan 2021

Production Optimization Of Porcine Reproductive And Respiratory Syndrome (Prrs) Virus Nucleocapsid Protein In Nicotiana Benthamiana, Boonyasit Porngarm

Chulalongkorn University Theses and Dissertations (Chula ETD)

Porcine Reproductive and Respiratory Syndrome virus (PRRSV) is a considerable pathogen to occur disease in pigs and effect to economic on the swine industry around the world including Thailand. Routine observation, rapid diagnosis of PRRSV infection is essential for effective monitoring and disease control. The serological assays using highly conserved viral nucleocapsid (N) protein are widely used for the detection of antibodies to the PRRSV. Accordingly, this study was aimed to produce the N-protein of the PRRSV in the plant system by using Nicotiana benthamiana. The N-protein gene was designed into four constructs that contained with and without the plant …

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Antioxidant Activity Of Thunbergia Laurifolia Lindl. Ethanolic Extract In Keratinocytes, Buakotchaphan Jirabanjerdsiri Jan 2021

Antioxidant Activity Of Thunbergia Laurifolia Lindl. Ethanolic Extract In Keratinocytes, Buakotchaphan Jirabanjerdsiri

Chulalongkorn University Theses and Dissertations (Chula ETD)

Fine particulate matter (PM2.5) can cause skin damage through the induction of oxidative stress in epidermis. Antioxidants help counteract with cellular oxidant species and maintain cell homeostasis. This study aims to examine the protective effect of Thunbergia laurifolia lindl. ethanolic extract on PM2.5-mediated oxidative stress in epidermal keratinocytes. High-performance liquid chromatography with diode array detection was conducted for quantitative analysis of rosmarinic acid content in the extract. Cell viability was detected by the 3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium Bromide assay. Intracellular ROS was monitored using 2’,7’-Dichlorodihydrofluorescein diacetate. Proteins related to p62-Keap1-Nrf2 signaling pathway were examined by western blot analysis. Expressions of Nrf2 and p62 …

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Antioxidant With Antimelanogenic Activities Of Sericin Hydrolysates Optimized By Response Surface Methodology In Human Melanin-Generating Mnt-1 Cells, Keerati Joyjamras Jan 2021

Antioxidant With Antimelanogenic Activities Of Sericin Hydrolysates Optimized By Response Surface Methodology In Human Melanin-Generating Mnt-1 Cells, Keerati Joyjamras

Chulalongkorn University Theses and Dissertations (Chula ETD)

Sericin, a protein presenting in the wastewater from silk industry, causes water pollution and ecological problem. To increase economic value to this waste product, the optimum enzymatic condition that could create sericin hydrolysates with high antioxidant and antimelanogenic capacities was generated through response surface methodology (RSM). Response surface plots demonstrate the major role of temperature on scavenging capacity of sericin hydrolysates assessed via DPPH, FRAP and ORAC assays. Alcalase®-hydrolyzed sericin consisted of sericin-related peptides in differing amounts and smaller sizes compared with unhydrolyzed sericin, as respectively demonstrated by peptidomic and SDS-PAGE analysis. Pre-incubation with 20 mg/mL sericin hydrolysates digested by …

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Development Of Plant-Produced Subunit Vaccine And Therapeutic Protein For Covid-19, Konlavat Siriwattananon Jan 2021

Development Of Plant-Produced Subunit Vaccine And Therapeutic Protein For Covid-19, Konlavat Siriwattananon

Chulalongkorn University Theses and Dissertations (Chula ETD)

Due to the rapid transmission of the Coronavirus Disease 2019 (COVID-19) causing serious public health problems and economic burden, the development of effective therapeutic interventions is urgently needed for controlling the ongoing pandemic disease. In this study, we have transiently produced recombinant SARS-CoV-2 RBD and human ACE2 fused with the Fc region of human IgG1 in Nicotiana benthamianain in order to use as a preventive subunit vaccine and therapeutic against SARS-CoV-2. The recombinant SARS-CoV-2 RBD-Fc subunit vaccine was formulated with different commercially available adjuvants including aluminium hydroxide gel (Alum), AddaVaxTM (MF59), monophosphoryl lipid A from Salmonella Minnesota R595 (mPLA-SM), and …

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Taxonomy And Biological Activities Of A Novel Saccharothrix Species From Soil Of Sichang Island, Md Abul Kalam Azad Jan 2021

Taxonomy And Biological Activities Of A Novel Saccharothrix Species From Soil Of Sichang Island, Md Abul Kalam Azad

Chulalongkorn University Theses and Dissertations (Chula ETD)

Actinobacteria are the sources of bioactive natural products. Rare actinobacteria are the less frequently isolated genera of actinobacteria. During an investigation of diversity of rare actinobacteria, actinobacteria isolate SC076T was isolated from a soil sample collected from Sichang Island, Chonburi Province, Thailand. The strain showed the highest 16S rRNA gene similarity to Saccharothrix australiensis DSM 43800T (98.58%) and Saccharothrix espanaensis DSM 44229T (98.58%). The zigzag morphology of the spore chain was observed on the aerial mycelia. meso-diaminopimelic acid was detected in the peptidoglycan. Whole-cell sugars contained rhamnose, ribose, mannose glucose and galactose. Polar lipids are phosphatidylethanolamine, hydroxyphosphatidylethanolamine, diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol …

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Effect Of Hydroquinone 5-O-Cinnamoyl Ester Of Renieramycin M On The Suppression Of Lung Cancer Stem Cells, Nattamon Hongwiangchan Jan 2021

Effect Of Hydroquinone 5-O-Cinnamoyl Ester Of Renieramycin M On The Suppression Of Lung Cancer Stem Cells, Nattamon Hongwiangchan

Chulalongkorn University Theses and Dissertations (Chula ETD)

Cancer stem cells (CSCs) are distinct cancer populations with tumorigenic and self-renewal abilities. CSCs are drivers of cancer initiation, progression, therapeutic failure, and disease recurrence. Thereby, novel compounds targeting CSCs offer a promising way to control cancer. In this study, the hydroquinone 5-O-cinnamoyl ester of renieramycin M (CIN-RM) was demonstrated to suppress lung cancer CSCs. CIN-RM was toxic to lung cancer cells with a half-maximal inhibitory concentration around 15 µM. CIN-RM suppressed CSCs by inhibiting colony and tumor spheroid formation. In addition, the CSC population was isolated and treated and the CSCs were dispatched in response to CIN-RM within 24 …

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Α-Glucosidase Inhibitors From Dendrobium Delacourii, Dendrobium Gibsonii And Aerides Multiflora, May Thazin Thant Jan 2021

Α-Glucosidase Inhibitors From Dendrobium Delacourii, Dendrobium Gibsonii And Aerides Multiflora, May Thazin Thant

Chulalongkorn University Theses and Dissertations (Chula ETD)

In this study, three plants from the Orchidaceae family, i.e., Dendrobium delacourii, Dendrobium gibsonii, and Aerides multiflora, were investigated for their chemical constituents and α-glucosidase inhibitory activities. A total of thirty compounds were isolated and structurally characterized. Eleven known compounds were identified from Dendrobium delacourii, including hircinol, ephemeranthoquinone, densiflorol B, moscatin, 4,9-dimethoxy-2,5-phenanthrenediol, gigantol, batatasin III, lusianthridin, 4,4',7,7'-tetrahydroxy-2,2'-dimethoxy-9,9',10,10'-tetrahydro-1,1'-biphenanthrene, phoyunnanin E, and phoyunnanin C. Two new compounds, i.e., dihydrodengibsinin and dendrogibsol, were isolated from Dendrobium gibsonii, along with seven known compounds including ephemeranthol A, dengibsinin, nobilone, aloifol I, lusianthridin, denchrysan A, and 4-methoxy-9H-fluorene-2,5,9-triol. Four new compounds, i.e., aerimultins A-C and dihydrosinapyl dihydroferulate, …

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Lipase Inhibitors From Dendrobium Senile, Myat Pann Phyu Jan 2021

Lipase Inhibitors From Dendrobium Senile, Myat Pann Phyu

Chulalongkorn University Theses and Dissertations (Chula ETD)

Obesity is characterized as an abnormal lipid metabolism due the imbalance between the energy intake and output. Pancreatic lipase is secreted by the pancreas and plays an important role in the digestion and absorption of lipids in the small intestine. Inhibition of pancreatic lipase enzyme can reduce dietary fat absorption which will lead to loss of weight and finally result in hypocholesterolemia and hypoglycemia. From the whole plant of Dendrobium senile, a new phenanthrene derivative was isolated, together with seven known compounds, including 2,5,7-trihydroxy-4-methoxyphenanthrene (1), moscatin (2), 2,5-dihydroxy-4,9-dimethoxyphenanthrene (3), moscatilin (4), aloifol I (5), 4,4ʹ,8,8ʹ-tetramethoxy[1,1ʹ-biphenanthrene]-2,2ʹ,7,7ʹ-tetrol (6), 2,2ʹ,7,7ʹ-tetrahydroxy-4,4ʹ-dimethoxy-1,1ʹ-biphenanthrene (7) and bleformin …

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Identification, Antifungal And Plant Growth Promoting Activities Of Endophytic Actinomycetes From Orchids, Nisachon Tedsree Jan 2021

Identification, Antifungal And Plant Growth Promoting Activities Of Endophytic Actinomycetes From Orchids, Nisachon Tedsree

Chulalongkorn University Theses and Dissertations (Chula ETD)

Sixty-two endophytic actinomycetes were isolated from 15 Thai orchids. They were identified as Streptomyces (34 isolates), Micromonospora (21 isolate), Streptosporangium (2 isolates), Actinomadura (1 isolates), Amycolatopsis (3 isolates) and Pseudonocardia (1 isolates) based on their phenotypic, chemotaxonomic and genotypic characteristics. The antimicrobial activity screening revealed that Streptomyces (18 isolates), Micromonospora (12 isolates) and Streptosporangium (1 isolate) exhibited antimicrobial activity against Bacillus subtilis ATCC 6633, Kocuria rhizophila ATCC 9341, Staphylococcus aureus ATCC 25923, Escherichia coli ATCC 25922, Pseudomonas aeruginosa ATCC 27853 and Candida albicans ATCC 10231. Strain DR2-2 could inhibit all tested pathogens. Based on 16S rRNA gene sequencing, strain DR6-1 …

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Extraction Of Cannabinoids Using Vegetable Oils And Its Uva-Photoprotective Effect On Human Skin Keratinocytes, Nur Alfan Muhammad Zen Jan 2021

Extraction Of Cannabinoids Using Vegetable Oils And Its Uva-Photoprotective Effect On Human Skin Keratinocytes, Nur Alfan Muhammad Zen

Chulalongkorn University Theses and Dissertations (Chula ETD)

Nowadays, vegetable oil has attracted attention in its role as an alternative solvent. Its ability to dissolve various active compounds from plants has been proven by several previous studies. Moreover, other benefits of the use of vegetable oil as a solvent are affordable, non-toxic, and eco-friendly. The extraction process is one of the crucial factors that affect the quality of cannabis. Ethanol is still consistently the best solvent for cannabis extraction in terms of yield production compared to other organic solvents used in this study including oil whose yield cannot be calculated since vegetable oil cannot be evaporated. HPTLC fingerprint …

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Production And Characterization Of Escherichia Coli Outer Membrane Vesicles Displaying Anti-Muc1 Single-Chain Variable Fragment Via Spytag/Spycatcher System, Sedthawut Laotee Jan 2021

Production And Characterization Of Escherichia Coli Outer Membrane Vesicles Displaying Anti-Muc1 Single-Chain Variable Fragment Via Spytag/Spycatcher System, Sedthawut Laotee

Chulalongkorn University Theses and Dissertations (Chula ETD)

Outer membrane vesicles (OMVs) secreted from Gram-negative bacteria have been discovered for a decade. Special features like high stability, ease of production, and intrinsic adjuvanticity highlight the use of OMVs in diverse applications such as vaccination, bioimaging, and multifunctional delivery systems. Synthetic biological approaches can functionalize OMVs to present heterologous proteins by expressing genetic fusion of target protein to outer membrane proteins. However, complex proteins cannot be incorporated into the OMVs due to lack of some post-translational modifications in bacteria. Herein, we demonstrated a convenient method for presentation of versatile proteins in OMVs with the implementation of the bio-ligation system …

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3,4-Dihydroxy-5,4’-Dimethoxybibenzyl Sensitizes Non-Small Cell Lung Cancer Cells To Cisplatin-Induced Apoptosis Via P53 Upregulation, Hardyanti Eka Putri Jan 2021

3,4-Dihydroxy-5,4’-Dimethoxybibenzyl Sensitizes Non-Small Cell Lung Cancer Cells To Cisplatin-Induced Apoptosis Via P53 Upregulation, Hardyanti Eka Putri

Chulalongkorn University Theses and Dissertations (Chula ETD)

Lung cancer is a leading fatal malignancy with the highest number of cancer deaths. Targeting a specific protein regulating cancer progression and metastasis has been attracting much attention for the development of cancer therapy. Thus, this study was aimed to investigate the effect of 3,4-dihydroxy-5,4′-dimethoxybibenzyl (DS-1) in targeting MDM2-attenuating p53 function and inhibition EMT in lung cancer cells. The efficacy of DS-1 or combined with cisplatin in lung cancer cells was determined by MTT, nuclear staining, and Annexin V/PI assays. The expression of apoptosis- and EMT-related proteins was determined by western blot analysis. Metastatic behaviors were evaluated by cell shape …

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Cross-Cultural Adaptation And Psychometric Properties Of The Thai Version Of The Neurophysiology Of Pain Questionnaire, Pichet Pasangkayo Jan 2021

Cross-Cultural Adaptation And Psychometric Properties Of The Thai Version Of The Neurophysiology Of Pain Questionnaire, Pichet Pasangkayo

Chulalongkorn University Theses and Dissertations (Chula ETD)

Background: Pain neurophysiology knowledge is hypothesized to influence pain beliefs and physical performance in individuals with chronic low back pain (LBP). Valid and reliable measures of such knowledge is important to evaluate the pain treatment on this domain as well as to understand its role in both physical and psychological functions of individuals with chronic pain. This study aimed to culturally adapt the revised Neurophysiology of Pain Questionnaire into Thai (T-rNPQ), using the Functional Assessment of Chronic Illness Therapy (FACIT) methodology, and to evaluate its reliability and validity. Two hundred sixty-three individuals with chronic LBP completed the T-rNPQ and seven …

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