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2021

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Production And Characterization Of Mannanase From Aureobasidium Sp. For Mannooligosaccharide Preparation From Spent Coffee Ground, Syahriar Nur Maulana Malik Ibrahim Jan 2021

Production And Characterization Of Mannanase From Aureobasidium Sp. For Mannooligosaccharide Preparation From Spent Coffee Ground, Syahriar Nur Maulana Malik Ibrahim

Chulalongkorn University Theses and Dissertations (Chula ETD)

The aims of this study are to produce and characterize mannanase from the selected Thai Aureobasidium strain and use the enzyme for hydrolysis of spent coffee ground extract (SCGE) to prepare mannooligosaccharide (MOs). The optimum condition for SCGE extraction by hot water was 1:30 solid to liquid ratio with 60 min incubation time at 121°C 15 lbs per in2. The maximum yield was 9.5 g/100 g substrate. The second dominant composition of SCGE was hemicellulose at 28 %. Aureobasidium pullulans NRRL 58524 was selected as the best mannanase producer with 8.07 U/mL. The mannanase production medium was optimized by using …

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Inhibitory Effects Of Propiconazole On Rice Root Development And Abilities Of Brassinosteroid And Auxin In Alleviating Its Effects, Watcharapong Wimonchaijit Jan 2021

Inhibitory Effects Of Propiconazole On Rice Root Development And Abilities Of Brassinosteroid And Auxin In Alleviating Its Effects, Watcharapong Wimonchaijit

Chulalongkorn University Theses and Dissertations (Chula ETD)

Propiconazole is commonly used as a fungicide to minimize crop loss caused by various fungal diseases in many crop plants, including rice. Propiconazole has the potentials to deteriorate the environmental problems due to its chemical properties that it is highly persistent in soil after application, suggesting that the constant use of propiconazole could be problematic. Hence, the evaluation of its effects on plant growth and development is necessary for the assessment of future use of propiconazole. Here, we demonstrated that propiconazole had inhibitory effects on plant growth. Treatments of propiconazole resulted in shoot dwarfism, abnormal leaf morphology, reduced plant biomass, …

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Liquid-Phase Selective Hydrogenation Of Furfural To Furfuryl Alcohol Over Pt- And Ptfe-Incorporated Mesoporous Carbon, Sureeporn Saknaphawuth Jan 2021

Liquid-Phase Selective Hydrogenation Of Furfural To Furfuryl Alcohol Over Pt- And Ptfe-Incorporated Mesoporous Carbon, Sureeporn Saknaphawuth

Chulalongkorn University Theses and Dissertations (Chula ETD)

This research aims to investigate the liquid-phase selective hydrogenation of furfural to furfuryl alcohol in a batch reactor at 50ºC, 20 bar H2 using water and methanol as the solvent. This study has been divided into two sections. The characteristics and catalytic properties of OMC-supported Pt catalysts (Pt/OMC-one-pot) prepared by one-step modified soft-template self-assembly method were compared with the Pt impregnated on OMC, activated carbon (AC), and non-uniform meso/macroporous carbon (MC) in the first section. Larger Pt particle size (~4 nm) was obtained on the Pt/OMC-one-pot comparing to all the impregnated ones, in which the Pt particle sizes were in …

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Mesoporous Carbon/Silica Nanocomposites With Acid And Base Properties For Glucose Conversion Into Value Added Chemicals, Satit Yousatit Jan 2021

Mesoporous Carbon/Silica Nanocomposites With Acid And Base Properties For Glucose Conversion Into Value Added Chemicals, Satit Yousatit

Chulalongkorn University Theses and Dissertations (Chula ETD)

The purpose of this study is to prepare mesoporous carbon/silica (MCS) nanocomposites using natural rubber (NR) as a renewable carbon source. A series of mesoporous nanocomposites based on NR and hexagonal mesoporous silica (HMS), prepared via an in situ sol-gel process, were used as precursors, which were then converted into the MCS materials by carbonization. The sulfonic acid-functionalized mesoporous carbon/silica (MCS-SO3H) was prepared by direct sulfonation with H2SO4 solution. The silica surface of MCS-SO3H were then grafted by 3-aminopropyltrimethoxysilane to produce the acid–base catalysts (MCS-SO3H-NH2) and used as the solid catalysts in glucose conversion. The MCS nanocomposites exhibited enhanced structural, …

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Chemical Constituents Of Fan-Si Abutilon Indicum Stems, Arum Restu Widyasti Jan 2021

Chemical Constituents Of Fan-Si Abutilon Indicum Stems, Arum Restu Widyasti

Chulalongkorn University Theses and Dissertations (Chula ETD)

This study aimed to isolate secondary metabolites from the stems of Abutilon indicum and to evaluate their α-glucosidase, pancreatic lipase, α-chymotrypsin inhibitory activity. Chromatographic fractionation of the n-hexane crude extracts led to the isolation of three known metabolite (1-3). The crude extract from all parts of the plant were evaluated for inhibitory activity on the alpha-glucosidase enzyme. The DCM-soluble roots were active with 10.2% at 25 μg/mL. The hexane and MeOH-soluble stems were active with 11.0% at 25 μg/mL and 8.0% at 0.25 μg/mL. The hexane, DCM, and MeOH-soluble leaves are active with 20.2% at 0.025 μg/mL; 20.0% at 0.025 …

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Impact Analysis Of Variable Renewable Energy Integration On Total System Costs And Electricity Generation Revenue And Impact Mitigation, Veeraya Imcharoenkul Jan 2021

Impact Analysis Of Variable Renewable Energy Integration On Total System Costs And Electricity Generation Revenue And Impact Mitigation, Veeraya Imcharoenkul

Chulalongkorn University Theses and Dissertations (Chula ETD)

Variable renewable energy (VRE) integration creates additional costs, called integration costs. These costs have grown with VRE penetration, potentially increasing the total system costs delivered to customers (direct integration costs) and decreasing electricity generation revenue, discouraging investment by generators (indirect integration costs). Thus, integration costs can serve as an economic barrier to the integration of high VRE shares. The main objective of this dissertation is to propose a novel method of determining the impacts of VRE integration on total system costs and electricity generation revenue. With this method, the optimal generation mix and optimal generation schedules at the specific VRE …

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Fuzzy Ahp For Supplier Selection: A Case Study In An Electronic Component Manufacturer, Chia Ken Tsai Jan 2021

Fuzzy Ahp For Supplier Selection: A Case Study In An Electronic Component Manufacturer, Chia Ken Tsai

Chulalongkorn University Theses and Dissertations (Chula ETD)

Supplier selection has become an essential effect on the entire electronic supply chain network on performance. The case study company produces a nano sim-card connector which four primary raw materials are processed into four primary parts (Plastic, Nickel, Phosphor bronze, and Stainless steel). Nevertheless, the case study company faces a penalty and order reduction because of the quality issue. Although an appraisal record from the case study company is able to select a proper raw material supplier, the cost becomes the priority when the candidate suppliers are categorized as the same level, leading to increasing potential risks, e.g., a penalty, …

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Environmental Ethics, Survival And Apocalypse: An Analysis Of Margaret Atwood’S Maddaddam Trilogy, Sarawut Kururatphan Jan 2021

Environmental Ethics, Survival And Apocalypse: An Analysis Of Margaret Atwood’S Maddaddam Trilogy, Sarawut Kururatphan

Chulalongkorn University Theses and Dissertations (Chula ETD)

This thesis examines the connection of ethics and survival in the pre- and post-apocalyptic worlds in Margaret Atwood's MaddAddam trilogy: Oryx and Crake (2003), The Year of the Flood (2009), and MaddAddam (2013). It investigates the author’s imaginings of a dystopian society marked by rampant technocratic capitalism and unchecked scientism in Oryx and Crake. Applying Murray Bookchin's social ecology to shed light on the root causes of the apocalypse, it argues that hierarchical ideology and diversiform inequality deeply rooted in society lead to warped ethics, affecting the crisis. This thesis also examines environmental ethics which is embedded in the belief …

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Sustainable Livelihood Of Fishermen: A Case Study In The City Of Semarang, Indonesia, Adam Prayuda Jan 2021

Sustainable Livelihood Of Fishermen: A Case Study In The City Of Semarang, Indonesia, Adam Prayuda

Chulalongkorn University Theses and Dissertations (Chula ETD)

The study aims to look at the social and economic circumstances of fishermen in Semarang City, their local livelihoods, and the significant role of the state through Semarang Fisheries Service to improve the economic livelihoods and social welfare of this marginal group. It applies a sustainable livelihood framework to look at the compatibility and empowerment process that can affect the Fishermen's Community. This research used mixed methods to understand the problem and apply narrative techniques for case studies to obtain the data. Different sources of information are used to triangulate information, perspectives, and expected results to increase knowledge in providing …

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Renewable Energy Transition Towards Krabi’S Sustainable Energy, Chariya Senpong Jan 2021

Renewable Energy Transition Towards Krabi’S Sustainable Energy, Chariya Senpong

Chulalongkorn University Theses and Dissertations (Chula ETD)

The present study was conducted with an aim to investigate Krabi's electricity outlook, renewable energy transition timeline, and potential of renewable energy resources, key drivers, barriers, and challenges towards Krabi's sustainable energy. Guideline as well as policy recommendation for successful renewable energy transition was also developed. The study found that Krabi's electricity demand has continuously increasing and tends to increase up to 320 MW by the year 2037. Meanwhile, electricity supply has still relied on national grid since 1995. Meanwhile, Krabi Goes Green which is a provincial roadmap has set a target to be self-reliance and all electricity supply would …

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Developing A System Of Community-Based Agritourism For Sustainable Local Food Systems: A Multi-Case Study Of Rural Livelihood Diversification And Sustainable Urban Consumption In City-Regional Bangkok, Sofia Anna Enrica Cavalleri Jan 2021

Developing A System Of Community-Based Agritourism For Sustainable Local Food Systems: A Multi-Case Study Of Rural Livelihood Diversification And Sustainable Urban Consumption In City-Regional Bangkok, Sofia Anna Enrica Cavalleri

Chulalongkorn University Theses and Dissertations (Chula ETD)

Current food systems fail to directly link rural producers and urban consumers. This research explores and categorizes emerging community-based agritourism practices as strategies to reconnect rural food producers with urban consumers. The main research question of this study is: how can community-based agritourism link rural food producers and urban consumers as a rural livelihood diversification strategy? Mixed methods for data collection were selected to answer this question and analyzed with a deductive and inductive approach. These include the review of secondary grey and academic literature, shadow observation in three rural provinces, content validity index calculation performed by experts (n = …

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Effects Of Configuration And Operating Conditions On Turbidity Removal Of Jet Clarifier, Ploypailin Romphophak Jan 2021

Effects Of Configuration And Operating Conditions On Turbidity Removal Of Jet Clarifier, Ploypailin Romphophak

Chulalongkorn University Theses and Dissertations (Chula ETD)

In water treatment, flocculation creates large and weighty flocs enough to be removed by the downstream processes of sedimentation and filtration. Among the various existing technologies, the jet clarifier is considered as an effective and compact system as it couples flocculation and clarification in a single unit. For the design of jet mixing, much experimental work has been done and many correlations have been proposed. However, these correlations are case specifics, and, to date, there is no comprehensive view for the flocculation aspect. In order to evaluate the performance of the jet clarifier for turbidity removal and understand hydrodynamics to …

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Football As A Tactical Innovation For Mobilization: A Study Of Fc Barcelona And The Catalan Independence Movement, Phawin Seangkumchoo Jan 2021

Football As A Tactical Innovation For Mobilization: A Study Of Fc Barcelona And The Catalan Independence Movement, Phawin Seangkumchoo

Chulalongkorn University Theses and Dissertations (Chula ETD)

The Catalan Independence Movement is the movement in the Northern region of Spain which aims to be independent from Spain cause of problems in politics and economic. The Catalan mobilization not only marches on the street but also plays their role through the Spanish political procedure. In Catalan, the referendum on independence was issued in 2009-2011, 2014, and 2017. This study focuses on the Catalan mobilization tactical innovation which uses football as a tool against Spain. In this case, football which was used mean ‘FC Barcelona,’ one of the famous football club in the world. This study aims to identify …

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Eu-Vietnam Fta: Implications For Thailand, Uthaipan Promkhatkaew Jan 2021

Eu-Vietnam Fta: Implications For Thailand, Uthaipan Promkhatkaew

Chulalongkorn University Theses and Dissertations (Chula ETD)

In 2007, the EU launched a region-to-region Free Trade Agreement (FTA) with the ASEAN; however, negotiations were suspended in 2009, and the EU subsequently decided to negotiate bilateral FTAs with individual ASEAN member states. Thailand, a parliamentary democratic country, launched the first round of trade negotiations with the EU in 2013, but they were put on hold due to the military coup in 2014. On the other hand, the EU still continued to negotiate and ultimately concluded the FTA with Vietnam in 2020 despite the fact that Vietnam is one of the world’s five remaining communist states. This has raised …

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Effects Of Ferulic Acid And Uv Curing On Properties Of Soy Protein Film, Md Shakil Jan 2021

Effects Of Ferulic Acid And Uv Curing On Properties Of Soy Protein Film, Md Shakil

Chulalongkorn University Theses and Dissertations (Chula ETD)

The objective of this study was to explore the effect of UV-C curing on the properties of ferulic acid-added soy protein film. The films were fabricated from soy protein isolate and added with 1.5% ferulic acid. UV-C radiation was applied at four different doses (0.32, 1.56, 4.00, 12.00 J/cm2) to either preformed film or film-forming solution. The mechanical, physicochemical, and morphological properties of the film samples were investigated. Ferulic acid addition and UV-C curing at 0.32 J/cm2 posed a significant effect on film thickness while film density was slightly affected by ferulic acid addition and/or UV-C treatment. UV-C irradiation of …

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Analysis Of Ancient Storm Deposits Along Coastal Areas Of Andaman Sea, Thailand And Coastal Areas Of South China Sea, Vietnam, Stapana Kongsen Jan 2021

Analysis Of Ancient Storm Deposits Along Coastal Areas Of Andaman Sea, Thailand And Coastal Areas Of South China Sea, Vietnam, Stapana Kongsen

Chulalongkorn University Theses and Dissertations (Chula ETD)

The frequency of high energy storm and tsunami events is crucial for apprehending the vulnerability of coastal communities. Identifying and dating sedimentary evidence deposited by such high energy events can assist in the planning and installation of suitable protection measurements. The Andaman Sea coast of Thailand is particularly vulnerable to such events as illustrated by the 2004 Indian Ocean Tsunami (2004 IOT) and the South China Sea coast of Vietnam has been experienced from the past severe storm event. Here, the authors study the high energy sediments. The selected area of Andaman Sea coast Thailand is Laem Son National park, …

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Assessments Of Chromium Pollution In Surface Sediment Of The Inner Gulf Of Thailand, Patcha Leelakun Jan 2021

Assessments Of Chromium Pollution In Surface Sediment Of The Inner Gulf Of Thailand, Patcha Leelakun

Chulalongkorn University Theses and Dissertations (Chula ETD)

The inner Gulf of Thailand is an important marine resource for economic and social developments of Thailand. Moreover, the inner Gulf of Thailand is a diverse of biological coastal ecosystem, which is make an equilibrium marine environment. On the other hand, this area is due mainly to a variety of pollutant sources from the surface runoff, particularly major large rivers including the Mae Klong, the Tha Chin, the Chao Phraya and the Bangpakong Rivers. As a result, chromium contamination can occur in the inner Gulf of Thailand and ultimately become a serious environmental issue in Thailand. Therefore, the surface sediments …

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Queering The Migrant Experience: Gay Filipino Workers’ Performance Of Gender And Sexual Identities In Bangkok, Hans Kevin Madanguit Jan 2021

Queering The Migrant Experience: Gay Filipino Workers’ Performance Of Gender And Sexual Identities In Bangkok, Hans Kevin Madanguit

Chulalongkorn University Theses and Dissertations (Chula ETD)

The Philippine migration scholarship heavily focuses on heteronormative issues that depict the migrant workers as heterosexuals. Due to this trend, there is a scarcity of studies on overseas Filipino workers who identify as part of the Lesbian, Gay, Bisexual, Transgender, and Queer (LGBTQ+) Community. Hence, this qualitative research focuses on eleven documented Filipino workers in Thailand who are males assigned at birth and identify as gay homosexuals. Individual informal interviews were employed to investigate how they practice their non-normative gender and sexuality, given their multiple yet intersectional social identities in a country with vibrant queer cultures. It also probes the …

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Epigenetic Regulation Of Reactive Oxygen Species-Induced Tumor Progression In Hepatocellular Carcinoma, Suchittra Phoyen Jan 2021

Epigenetic Regulation Of Reactive Oxygen Species-Induced Tumor Progression In Hepatocellular Carcinoma, Suchittra Phoyen

Chulalongkorn University Theses and Dissertations (Chula ETD)

Oxidative stress is a consequence of an imbalance of antioxidants and reactive species. The most common form of reactive species is derived from oxygen, called reactive oxygen species (ROS). ROS involve in pathogenesis of several diseases including cancers. Cancer genesis and progression are contributed through both genetic and epigenetic mechanisms. Histone modification, a post-translational modification at histone tails, is one of the epigenetic mechanisms known to participate in carcinogenesis and tumor progression. Although the ROS-induced histone modification alteration has been demonstrated in some cancers, the change in histone methylation and gene expression by ROS in hepatocellular carcinoma (HCC) is scarcely …

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A Comparative Study Of Gut Microbiota In The Healthy Elderly And The Elderly With Colorectal Cancer, Nutta Iadsee Jan 2021

A Comparative Study Of Gut Microbiota In The Healthy Elderly And The Elderly With Colorectal Cancer, Nutta Iadsee

Chulalongkorn University Theses and Dissertations (Chula ETD)

Colorectal cancer is one of the third most common cancer worldwide. Dysbiosis of the human gut microbiota has been linked to sporadic colorectal cancer (CRC). This study aimed to compare the gut microbiota profile of a total of 80 Thai volunteers, who were above 50 years old, among 25 CRC patients, 33 adenoma patients, and 22 healthy controls (HC). The 16S rRNA sequencing was utilized to characterize the gut microbiome in both mucosal tissue and stool samples. Moreover, absolute quantitative PCR (qPCR) assay was conducted to quantify six CRC-associated bacteria including Fusobacterium nucleatum (FN), Parvimonas micra (PM), colibactin positive strains …

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Endotoxemia From Nsaids-Induced Gut-Leakage Enhanced Lupus Characteristics In Fc Gamma Receptor Iib Deficient Lupus Mice, Thansita Bhunyakarnjanarat Jan 2021

Endotoxemia From Nsaids-Induced Gut-Leakage Enhanced Lupus Characteristics In Fc Gamma Receptor Iib Deficient Lupus Mice, Thansita Bhunyakarnjanarat

Chulalongkorn University Theses and Dissertations (Chula ETD)

High dose of non-steroidal anti-inflammatory drugs (NSAIDs), the common analgesia, might induce lupus activity through NSAIDs-induced gastrointestinal permeability defect (gut leakage) that causes endotoxemia. Indomethacin (25 mg/day) was orally administered for 7 days in 24-week-old Fc gamma receptor IIb deficient (FcgRIIb-/-) mice, an asymptomatic lupus model, and age-matched wild-type (WT) mice. The severity of indomethacin-induced enteropathy in FcgRIIb-/- mice was higher than WT mice as demonstrated by intestinal injury (histology, immune-deposition and intestinal cytokines), gut leakage (FITC-dextran assay and endotoxemia) and serum cytokines. In addition, higher responses against endotoxemia in indomethacin-administered FcgRIIb-/- mice was also supported by the prominent responses …

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Dosimetric Impact Of Interplay Effects Instereotactic Body Radiation Therapy (Sbrt) Lung Cancer, Vanida Poolnapol Jan 2021

Dosimetric Impact Of Interplay Effects Instereotactic Body Radiation Therapy (Sbrt) Lung Cancer, Vanida Poolnapol

Chulalongkorn University Theses and Dissertations (Chula ETD)

Lung cancer patient breathing induced interplay effects in the volumetric modulated arc therapy (VMAT) together with stereotactic body radiation therapy (SBRT), which lead to non-uniform doses within the target volume and unwanted dose to the surrounding tissues. The purpose of this study was to investigate the dosimetric impact of interplay effects in 6 MV FFF of photon beams for lung cancer and to calculate the novel interplay effects factor (IEF) for VMAT-SBRT lung cancer. This study performed the static and dynamic measurements using two dimensions (2D) robotic platform and diode array for 3D-conformal radiation therapy (3D-CRT) plans, while using three …

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Study Of G6pd Deficiency With Clinical And Hematological Correlation In Newborn Infants, Thanaporn Pimpakan Jan 2021

Study Of G6pd Deficiency With Clinical And Hematological Correlation In Newborn Infants, Thanaporn Pimpakan

Chulalongkorn University Theses and Dissertations (Chula ETD)

G6PD deficiency is the most common enzymatic aberration, affecting more than a million people worldwide. If severe cases at birth are not diagnosed and treated promptly, they can develop into cerebral jaundice and hyperbilirubinemia. Hyperbilirubinemia is caused by an imbalance between increased bilirubin production and ineffective excretion of bilirubin. This can result in the neurotoxicity of bilirubin, kernicterus, and fatal mental retardation. Besides, It has been speculated that the presence of a high number of reticulocytes in newborns interferes with the diagnosis of G6PD deficiency since reticulocytes contain higher amounts of G6PD enzyme than mature erythrocytes. Therefore, the purposes of …

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A Pilot Study, Randomized Control Trial On Effect Of Oral Calcium Carbonate To Fecal Functional Levofloxacin Concentration In Healthy Volunteer Taking Oral Levofloxacin, Ophat Janphet Jan 2021

A Pilot Study, Randomized Control Trial On Effect Of Oral Calcium Carbonate To Fecal Functional Levofloxacin Concentration In Healthy Volunteer Taking Oral Levofloxacin, Ophat Janphet

Chulalongkorn University Theses and Dissertations (Chula ETD)

Objective: We conducted a pilot randomized control trial (RCT) to study whether oral calcium carbonate (CaCO3) can lower fecal levofloxacin (LVX) concentration and preserve gut microbiota diversity in healthy volunteers. Methods: The healthy volunteers received a 5-day course of once-daily 500 mg LVX oral tablet and were randomly assigned to treatment (6-day course of 1,000 mg CaCO3 oral tablet twice daily) and control group (no CaCO3). The primary outcome was fecal LVX concentration by MIC and high-performance liquid chromatography (HPLC) on day 2 and 5. The secondary outcomes were fecal microbiota diversity Shannon index (H) by 16s rDNA analysis, plasma …

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Tuning The Wettability Of Metal Surface With Electrodeposited Porous Copper And Graphene Coatings, Husam Eltigani Jan 2021

Tuning The Wettability Of Metal Surface With Electrodeposited Porous Copper And Graphene Coatings, Husam Eltigani

Chulalongkorn University Theses and Dissertations (Chula ETD)

Electrodeposition of porous copper is an interesting type of coating that is emerging for various applications. However, the understanding of the relationships between copper surface wettability control and electrodeposition parameters and post-electrodeposition storage conditions is quite limited. Furthermore, it is worthwhile to explore how nanomaterials such as graphene would interact with porous copper to provide surface wettability modifications. This work therefore aims to develop a framework for the relationship of copper and graphene deposition processes, storage conditions, and the wetting behavior of copper surfaces. The research work performed in this study is divided into 2 parts: (i) effects of plating …

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Effect Of Shear Force On Immunosuppressive Property Of Human Periodontal Ligament Cells, Ravipha Suwittayarak Jan 2021

Effect Of Shear Force On Immunosuppressive Property Of Human Periodontal Ligament Cells, Ravipha Suwittayarak

Chulalongkorn University Theses and Dissertations (Chula ETD)

The immunomodulatory property is to regulate immune cell function. IDO and TGF-β1 inhibited T cell proliferation and induced regulatory T (Treg) cells differentiation. However, this property must be activated by inflammation. Recently, mechanical forces have also activated this property. Hence, this study investigated effect of shear stress on immunosuppressive property of hPDL cells. Cells were subjected to shear stress then expression of IDO, TGF-β1, IFN-gamma and COX2 mRNA and protein was examined by qRT-PCR, ELISA, and IDO activity. Conditioned media derived shear stress were treated with CD4+ T cells. The proliferative T cell effect was determined using resazurin. The FOXP3, …

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Production Optimization Of Porcine Reproductive And Respiratory Syndrome (Prrs) Virus Nucleocapsid Protein In Nicotiana Benthamiana, Boonyasit Porngarm Jan 2021

Production Optimization Of Porcine Reproductive And Respiratory Syndrome (Prrs) Virus Nucleocapsid Protein In Nicotiana Benthamiana, Boonyasit Porngarm

Chulalongkorn University Theses and Dissertations (Chula ETD)

Porcine Reproductive and Respiratory Syndrome virus (PRRSV) is a considerable pathogen to occur disease in pigs and effect to economic on the swine industry around the world including Thailand. Routine observation, rapid diagnosis of PRRSV infection is essential for effective monitoring and disease control. The serological assays using highly conserved viral nucleocapsid (N) protein are widely used for the detection of antibodies to the PRRSV. Accordingly, this study was aimed to produce the N-protein of the PRRSV in the plant system by using Nicotiana benthamiana. The N-protein gene was designed into four constructs that contained with and without the plant …

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Identification, Antifungal And Plant Growth Promoting Activities Of Endophytic Actinomycetes From Orchids, Nisachon Tedsree Jan 2021

Identification, Antifungal And Plant Growth Promoting Activities Of Endophytic Actinomycetes From Orchids, Nisachon Tedsree

Chulalongkorn University Theses and Dissertations (Chula ETD)

Sixty-two endophytic actinomycetes were isolated from 15 Thai orchids. They were identified as Streptomyces (34 isolates), Micromonospora (21 isolate), Streptosporangium (2 isolates), Actinomadura (1 isolates), Amycolatopsis (3 isolates) and Pseudonocardia (1 isolates) based on their phenotypic, chemotaxonomic and genotypic characteristics. The antimicrobial activity screening revealed that Streptomyces (18 isolates), Micromonospora (12 isolates) and Streptosporangium (1 isolate) exhibited antimicrobial activity against Bacillus subtilis ATCC 6633, Kocuria rhizophila ATCC 9341, Staphylococcus aureus ATCC 25923, Escherichia coli ATCC 25922, Pseudomonas aeruginosa ATCC 27853 and Candida albicans ATCC 10231. Strain DR2-2 could inhibit all tested pathogens. Based on 16S rRNA gene sequencing, strain DR6-1 …

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Ab Initio Calculations On Superconducting Metal-Hydride And Perovskites For Energy Applications, Wiwittawin Sukmas Jan 2021

Ab Initio Calculations On Superconducting Metal-Hydride And Perovskites For Energy Applications, Wiwittawin Sukmas

Chulalongkorn University Theses and Dissertations (Chula ETD)

Superconducting hydrides and photovoltaic perovskites for energy applications are studied by means of the density functional theory-based calculations. First of all, the new decoration of Mg0.5Ca0.5H6 is confirmed thermodynamically stable over the pressures ranging from 200 to 400 GPa. The electronic properties reveal the possibility for this very material of becoming a superconductor under pressures. As a result, by performing electron-phonon coupling (EPC) calculations, Mg0.5Ca0.5H6 is found to superconduct at a maximum critical temperature of 288 K at 200 GPa, which completely exceeds those of its parent compounds, i.e. MgH6 and CaH6. Secondly, the van de Waals (vdW) interaction is …

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Effectiveness Of A Social Health Security Program In Improving Financial Risk Protection Against The Health Expenditures Of The Insured Populations In Four Districts Of Nepal: A Mixed Method Study, Gaj Bahadur Gurung Jan 2021

Effectiveness Of A Social Health Security Program In Improving Financial Risk Protection Against The Health Expenditures Of The Insured Populations In Four Districts Of Nepal: A Mixed Method Study, Gaj Bahadur Gurung

Chulalongkorn University Theses and Dissertations (Chula ETD)

Background: Most low and low-middle income (LMICs) countries adopting National Health Insurance (NHI) programs to achieve Universal Health Coverage are struggling to implement the program and thus have failed to achieve the expected outputs. The Nepal NHI program initiated in 2016 has experienced numerous implementation challenges, due to inadequate inputs and throughput, resulting in negative output such adverse selection, poor health service delivery, low enrollment of the poor household and low annual non-renewals of the NHI membership. However, there is a lack of research that focuses on these problems. So, this study has two objectives. Firstly, delved into the NHI …

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