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In Silico Drug Repositioning Against Human Nrp1 To Block Sars-Cov-2 Host Entry, Şeref Gül

Turkish Journal of Biology

Despite COVID-19 turned into a pandemic, no approved drug for the treatment or globally available vaccine is out yet. In such a global emergency, drug repurposing approach that bypasses a costly and long-time demanding drug discovery process is an effective way in search of finding drugs for the COVID-19 treatment. Recent studies showed that SARS-CoV-2 uses neuropilin-1 (NRP1) for host entry. Here we took advantage of structural information of the NRP1 in complex with C-terminal of spike (S) protein of SARSCoV- 2 to identify drugs that may inhibit NRP1 and S protein interaction. U.S. Food and Drug Administration (FDA) approved …

Binary-Qsar Guided Virtual Screening Of Fda Approved Drugs And Compounds In Clinical Investigation Against Sars-Cov-2 Main Protease, Lalehan Oktay, Ece Erdemoğlu, İlayda Tolu, Yeşi̇m Yumak, Ayşenur Özcan, Eli̇f Acar, Şehri̇ban Büyükkiliç, Alpsu Olkan, Serdar Durdaği

Turkish Journal of Biology

With the emergence of the new SARS-CoV-2 virus, drug repurposing studies have gained substantial importance. Combined with the efficacy of recent improvements in ligand- and target-based virtual screening approaches, virtual screening has become faster and more productive than ever. In the current study, an FDA library of approved drugs and compounds under clinical investigation were screened for their antiviral activity using the antiviral therapeutic activity binary QSAR model of the MetaCore/MetaDrug platform. Among 6733-compound collection, we found 370 compounds with a normalized therapeutic activity value greater than a cutoff of 0.75. Only these selected compounds were used for molecular docking …

The Current State Of Validated Small Molecules Inhibiting Sars-Cov-2 Non-Structural Proteins, Fati̇h Kocabaş, Merve Uslu

Turkish Journal of Biology

The current COVID-19 outbreak has had a profound influence on public health and daily life. Despite all restrictions and vaccination programs, COVID-19 still can lead to fatality due to a lack of COVID-19-specific treatments. A number of studies have demonstrated the feasibility to develop therapeutics by targeting underlying components of the viral proteome. Here we reviewed recently developed and validated small molecule inhibitors of SARS-CoV-2's nonstructural proteins. We described the validation level of identified compounds specific for SARS-CoV-2 in the presence of in vitro and in vivo supporting data. The mechanisms of pharmacological activity, as well as approaches for developing …

Coronavac (Sinovac) Covid-19 Vaccine-Induced Molecular Changes In Healthy Human Serum By Infrared Spectroscopy Coupled With Chemometrics, Ayça Doğan Mollaoğlu, Rafi̇g Gurbanov, Mete Severcan, Feri̇de Severcan

Turkish Journal of Biology

From the beginning of the COVID-19 coronavirus pandemic in December of 2019, the disease has infected millions of people worldwide and caused hundreds of thousands of deaths. Since then, several vaccines have been developed. One of those vaccines is inactivated CoronaVac-Sinovac COVID-19 vaccine. In this proof of concept study, we first aimed to determine CoronaVac-induced biomolecular changes in healthy human serum using infrared spectroscopy. Our second aim was to see whether the vaccinated group can be separated or not from the non-vaccinated group by applying chemometric techniques to spectral data. The results revealed that the vaccine administration induced significant changes …

Individual And Combined Effect Of Drought And Heat Stresses In Contrasting Potatocultivars Overexpressing Mir172b-3p, Arslan Asim, Zahi̇de Nesli̇han Öztürk Gökçe, Allah Bakhsh, İlknur Tindaş Çayli, Emre Aksoy, Sevgi̇ Çalişkan, Mehmet Emi̇n Çalişkan, Ufuk Demi̇rel

Turkish Journal of Agriculture and Forestry

MicroRNAs (miRNAs) are essential players of plant defence systems because of their involvement in reprogramming gene expression under adverse environmental conditions including drought and heat, which are considered major players in limiting crop productivity. miR172b-3p was previously determined as a remarkable stress-responsive miRNA in our next-generation sequencing (NGS) analysis in potato. This study aims to understand the functions of miR172b-3p and its target (ERTF RAP2-7-like) under drought, heat, and combined treatments by overexpressing the miR172b-3p in stress-tolerant (Unica) and sensitive (Russet Burbank) potato cultivars. miR172b-3p overexpression in transgenic lines suppressed the ERTF RAP2-7-like expression leading to enhanced carbon fixation efficiency. …

Cover And Contents

Turkish Journal of Agriculture and Forestry

No abstract provided.

Optimization Of Agrobacterium-Mediated Transformation And Regeneration For Crispr/Cas9 Genome Editing Of Commercial Tomato Cultivars, Zafer Seçgi̇n, Musa Kavas, Kubi̇lay Yildirim

Turkish Journal of Agriculture and Forestry

Tomato (Solanum lycopersicum) is the second most important horticultural crop worldwide that is widely used as a model plant in genetic manipulation of Solanaceae. CRISPR/Cas9 system has been successfully utilized in several studies for genome edition of model tomato cultivars. However, these genome editing systems should be also optimized for commercial tomato cultivar for direct application of genome editing in field conditions. In this study, we have optimized an Agrobacterium-mediated gene transfer and regeneration system for CRISPR/Cas9 genome editing in two commercial tomato cultivars for the first time. The effect of explant type, genotype, pre-transformation time, Agrobacterium concentration, infection time, …

Analysis Of Designs Used In Monitoring Crop Growth Based On Remote Sensing Methods, Cristina Teodora Dobrota, Rahela Carpa, Anca Butiuc-Keul

Turkish Journal of Agriculture and Forestry

Choosing appropriate designs and methods for monitoring crop growth is a challenging process of major importance. Remote sensing from space and manned or unmanned airborne operations are used to measure crop reflectance and a wide variety of other agricultural parameters. While some experiments use only a few, specific methods and designs and organize the results in lists of evidence, other experiments use a wider range of techniques to create a more credible and comprehensive assessment of crop yield. Particular situations related to the available resources in terms of data collection and expertise in addition to the intended use of the …

Morphological And Molecular Characterization Of Croatian Carob Tree(Ceratonia Siliqua L.) Germplasm, Snjezana Bolaric, Ivna Dragojevic Müller, Ales Vokurka, Dubravka Vitali Cepo, Mirko Ruscic, Sinisa Srecec, Dario Kremer

Turkish Journal of Agriculture and Forestry

The results of morphological and AFLP variability of 120 plants of carob tree (Ceratonia siliqua L.), collected from 12 different locations (10 biological replicates for each location) on the coast and islands of the southern Croatian Adriatic, indicate high molecular and morphological variability among these carob populations. Analysis of molecular variance revealed significant differences among populations (26.07%; p < 0.001; a = 0.05). Out of the total variability, 22.49% refers to the variability among, and 77.51% within populations. UPGMA and STRUCTURE analysis based on AFLP genetic data clustered carob populations into three main groups representing three real genetic populations. UPGMA analysis based on morphological traits of leaves, pods, and seeds clustered carob populations into five groups. Mantel test showed significant correlation between morphological and genetic data (r = 0.58, p < 0.001; a = 0.05). According to the high genetic and morphological variability, the germplasm collection in the analysis could represent an important genetic pool for future breeding programmes. The goal of future research should be the conservation of C. siliqua in its natural habitats, and the establishment of gene banks of genetic resources with the purpose of creating new carob cultivars in breeding programmes.

Evaluation Of Lonicera Caerulea L. Genotypes Based On Morphological Characteristics Offruits Germplasm Collection, Olga Grygorieva, Svitlana Klymenko, Alla Kuklina, Yulia Vinogradova, Olena Vergun, Vladimira Horcinova Sedlackova, Jan Brindza

Turkish Journal of Agriculture and Forestry

It is known that a polymorphic population consisting of a large number of genotypes adapts more rapidly to the environment conditions. The availability of a large gene pool predetermines the success of the introduction and further breeding of new cultivars with economically valuable features. Morphometrics is a classical tool for studying the amplitude of the variability of cultivated plants and evaluating the genotypic diversity of the artificial population. The objective of this study was to evaluate the morphological parameters of Lonicera caerulea L. fruits for 26 genotypes from the population introduced in the M.M. Gryshko National Botanical Garden (Kyiv). Morphological …

Multiplex Fluorescence Quantitative Pcr For The Detection Of Eight Bacterial Pathogens Ofporcine Respiratory Disease Complex (Prdc), Jingbo Wu, Wenjin Nan, Jiangqiang Huang, Guoliang Peng, Honghui Hu

Turkish Journal of Veterinary & Animal Sciences

As rapid and reliable tools for diagnosis of porcine respiratory disease complex (PRDC) bacterial pathogens are getting more important over time, we established a new multiplex fluorescence quantitative polymerase chain reaction (mFQ-PCR) system with a similar symptom. Our results showed that the system could accurately and simultaneously detect 8 pathogens within 1.5 h. The detection limits were 2-5 standard DNA copies. The correlation coefficient of the standard curve was more than 0.998, with a well dynamic range from 101 to 109, and the intra- and intervariation coefficients (CVs) were less than 2.06%. After setting the positive threshold of Ct value, …

Chemical Castration Using An Intratesticular Injection Of Mannitol: A Preliminary Studyin A Rat Model, Mohammad-Ali Maadi, Mehdi Behfar, Amir Rasaei, Ali Shalizar-Jalali, Gholamreza Najafi, Vahid Mohammadi

Turkish Journal of Veterinary & Animal Sciences

This study was aimed to evaluated chemical sterilization using an intra-testicular injection of mannitol 20% in rats. Fifty male Wistar rats were divided into five groups, including 1) intact, 2) surgical castration and, 3 to 5) intra-testicular injections of normal saline, hypertonic saline, and mannitol, respectively. The baseline testosterone levels were measured. Surgical castration was performed in group 2. Intra-testicular injections were performed in groups 3, 4, and 5. On day 60, the testosterone level was evaluated. Then, sperm count, viability, motility, morphological abnormalities and DNA damage were studied. Gonadosomatic index was recorded on day 60. Serum testosterone level was …

Comparison Of Feed Value Of Amaranthus Powellii Willd. Forage To Some Roughagefeeds, Gökhan Fi̇li̇k, Ayşe Gül Fi̇li̇k

Turkish Journal of Veterinary & Animal Sciences

The aim of this study was to compare the chemical composition, in vitro digestibility, protein and energy value, roughage value of Amaranthus powellii Willd. forage to the most commonly used alfalfa hay and wheat straw in ruminant nutrition. Amaranthus powellii Willd. forage has the potential to be a third quality roughage according to its relative feed value assessment of 104.55 ± 0.67. In addition, the relative forage quality assessment developed for feeding dairy cattle was 97.73 ± 0.05 for Amaranthus powellii Willd. forage, which had a higher the relative forage quality value than alfalfa hay and wheat straw, and it …

Effect Of Vanadium (Iv) Chloride Supplementation On Appetite-Related Hormone Levels Inrats With Experimentally Induced Diabetes, Tünay Kontaş Aşkar, Adnan Adi̇l Hi̇şmi̇oğullari, Olga Büyükleblebi̇ci̇, Hati̇ce Nur Er Sarikaya

Turkish Journal of Veterinary & Animal Sciences

Vanadium is one of the essential trace elements for mammals, which has important functions in the metabolism of carbohydrates. There is limited data about the effects of vanadium (IV) chloride supplementation on appetite-related hormones in literature. Our target was to analyse the impacts of vanadium supplementation on appetite-related hormone (leptin, nesfatin-1 and apelin) levels in rats with experimentally induced diabetes. Twenty eight male Sprague-Dawley albino rats were used in this study. After formation of diabetes, 0.3 mg/mL of vanadium was added to drinking water of rats for four weeks. A substantial increment was found in serum glucose (p < 0.001), HbA1c (p < 0.001), HOMA-IR (p < 0.01), and leptin (p < 0.001) levels and a significant decrease in insulin, apelin and nesfatin-1 concentrations in diabetic rats. Moreover, blood glucose, HbA1c, HOMA-IR, and leptin levels decreased in diabetic + vanadium group, but nesfatin-1 (1.74 ± 0.94 ng/mL), apelin (1.74 ± 0.94 ng/mL), and insulin concentrations were found higher compared with the diabetic group. As a result of this study, vanadium has increased the levels of circulating nesfatin-1 and apelin levels in diabetic rats while decreasing blood glucose and leptin concentrations. But further studies are required to determine the blood glucose lowering effects of vanadium and its relationship with appetite related hormones in diabet.

Detection Of Varroa Mites From Honey Bee Hives By Smart Technology Var-Gor: A Hivemonitoring And Image Processing Device, Sedat Sevi̇n, Hi̇dayet Tutun, Seli̇me Mutlu

Turkish Journal of Veterinary & Animal Sciences

The major honey bee parasite Varroa destructor reduces hive vitality and honey yields by preventing growth of heathy bees and causes drastic loss in apiculture. Therefore, mite infestation in honey bee needs to be constantly controlled by beekeepers. To minimize this loss, a system called Var-Gor has been developed with the aim of controlling Varroa mite infestation before/just after entering the hive instead of the late period. Var-Gor is a hive entrance attachable device box consist of bee passage tunnels (width: 25 mm, height: 15 mm, and depth: 50 mm), autofocus detection camera combined with interface (process sensor: IV-HG10) and …

Indigenous Entomopathogenic Fungi As Potential Biological Control Agents Of Rose Sawfly, Arge Rosae L. (Hymenoptera: Argidae), Derya Baki̇, Hi̇lal Şule Tosun, Fedai̇ Erler

Turkish Journal of Zoology

The rose sawfly, Arge rosae L. (Hymenoptera: Argidae), is one of the most destructive pests of roses grown both indoors and outdoors in Antalya province (south-western part of Turkey). Especially in greenhouse rose cultivation, growers depend heavily on synthetic pesticides to control this pest along with other arthropod pests in their crops. The aim of this study was to evaluate biocontrol potential of some indigenous soil-borne entomopathogenic fungi (EPF) against the pest. In pathogenicity assays, a total of 17 EPF isolates, belonging to three species [Beauveria bassiana (Bals.) Vuill. - 14, Clonostachys rosea (Link) Schroers - 2 and Isaria farinosa …

Infestation And Effect Of Parasitic Isopod Epipenaeon Ingens Ingens Nobili, 1906 Oncommercial Shrimp Species In The Eastern Mediterranean: A Case Study Of The Populationof Brown Shrimp Penaeus Aztecus Ives, 1891, Mehmet Cengi̇z Deval, Fatma Gonca Koçanci

Turkish Journal of Zoology

Monthly samples of seven species of penaeid shrimps, namely, Penaeus aztecus (Ives, 1891), Penaus hathor (Burkenroad, 1959) Penaus japonicus (Spence Bate,1888), Penaus kerathurus (Forskal,1775), Penaus semisulcatus (De Haan,1844), Metapenaeus monoceros (Fabricus, 1798) and Metapenaeopsis aegyptia (Galil & Golani, 1990) were collected from Antalya Bay in the Eastern Mediterranean Sea from March, 2019 through March, 2020. Except for P. aztecus and P. semisulcatus, no other shrimp species were infected with the parasitic isopod Epipenaeon ingens ingens. Of the 3043 P. aztecus shrimp inspected, 541 individuals were infected (17.8%) with the bopyrid parasite E. ingens ingens. However, the annual infestation rate in …

Identification Of Micrornas In The Follicular And Luteal Tissues Of Holstein-Friesian Cows, Selçuk Özdemi̇r, Seli̇m Çomakli

Turkish Journal of Veterinary & Animal Sciences

MicroRNAs (miRNAs) regulate many physiological pathways, including development, cell differentiation, immune response as well as diseases by post-transcriptional processes. However, information is limited regarding the biological roles of miRNAs in the development of the follicular and luteal tissues in cows. The main aim of the present study was to identify miRNAs that are expressed in the follicular and luteal tissues of cows. We used a comparative RNA sequencing method to identify miRNA candidates that might play key roles in the follicular and luteal phases of Holstein cows. Nine miRNAs were expressed at a high ratio: 8 in the follicular tissue …

The Ichthyofaunal Diversity Of Freshwater Ecosystems In Gökçeada Island (Nw Turkey)Under The Pressure Of Nonnative Species, Sevan Ağdamar, Gülşah Saç, Özcan Gaygusuz, Ersi̇n Doğaç, Ümi̇t Acar, Çi̇ğdem Gürsoy Gaygusuz, Müfi̇t Özuluğ

Turkish Journal of Zoology

Freshwater ecosystems have a greater value for biodiversity per surface area than terrestrial and marine ecosystems. Streams and lakes are notably prone to biodiversity loss, being the greatest threats habitat destructions and nonnative species. The aim of the present study is to assess the fish composition of lotic and lentic water systems of an island ecosystem (Gökçeada Island, Turkey), which is poorly studied, and to highlight the entry routes and possible impacts of translocated fish species in an island ecosystem. The field surveys were conducted in a total of 14 sampling sites (five reservoirs and nine streams) in the inland …

The Modern Use Of The Extended Humeral Head (Cuff Tear Arthropathy) Hemiarthroplasty., Tyler J Smith, Sarav S Shah, Justin W Peterson, Glen Ross

Orthopedic Surgery Resident Research

Today, the treatment of osteoarthritis in the rotator cuff-deficient population is largely dominated by reverse shoulder arthroplasty (RSA). Despite the popularity of and increased familiarity with this procedure, the complication rate of RSA remains significant. An extended humeral head hemiarthroplasty may provide a less invasive alternative for select patients with cuff tear arthropathy (CTA) and preserved glenohumeral active elevation. With the indications for reverse arthroplasty expanding to younger patients, there are concerns about the longevity of this implant, as well as the associated revision burden. In the setting of failed RSA, the bone stock available for glenosphere baseplate fixation can …

Incorporating Psychological Trauma Into Adhd Differentials: A Pilot Study Of Primary Care Providers' Perspectives, Amber Nipper

PsyD Program in Clinical Psychology Doctoral Dissertations (Seattle)

According to the American Academy of Pediatrics (AAP, n.d.), 40% to 50% of individuals seeking mental health services terminate prematurely due to lack of access, lack of transportation, financial constraints, child mental health professional shortages, and stigma related to mental health. These barriers contribute to primary care providers assessing and managing mental health concerns at increasing rates, particularly symptoms consistent with attention deficit/hyperactivity disorder (ADHD). ADHD is a neurobiological disorder beginning in childhood that is defined as “a persistent pattern of inattention and/or hyperactivity-impulsivity that interferes with functioning or development” (American Psychiatric Association [APA], 2013, p. 59). Current literature shows …

Digest Of The Philadelphia College Of Osteopathic Medicine (2021 No. 1), Philadelphia College Of Osteopathic Medicine

PCOM Digest 2020s

The 2021 No. 1 issue of the Digest of the Philadelphia College of Osteopathic Medicineincludes the following articles:

• Updates
• Institutional Heritage: Founders’ Day
• Meta L. Christy, DO: The First African American Osteopathic Physician
• Standing on Her Shoulders: Celebrating Meta L. Christy, DO, and African American Alumnae Trailblazers
• Class Notes

Does Experimentally Quieting Traffic Noise Benefit People And Birds?, Mitchell J. Levenhagen, Christopher J.W. Mcclure, Jesse R. Barber

Biology Faculty Publications and Presentations

Protected natural areas are not free from noise, especially noise generated by traffic within park boundaries. Natural soundscapes are important for maintaining community structure, providing positive visitor experiences, and increasing visitor support for management actions that reduce impacts on natural resources. To test experimental quieting as a strategy to increase both wildlife habitat quality and visitor experience, we enforced decreased speed limits and presented educational signage to reduce sound levels along a road system in an alternating, on–off block design within Grand Teton National Park, Wyoming, USA. We continuously recorded background sound levels while conducting bird space use assessments and …

Soils Varying Importance And Differences, Kylie Fouss

Natural Sciences Student Research Presentations

No abstract provided.

2021 Idea Of Nature Public Lecture Series Schedule (Flyer), Samantha Harvey

The Idea of Nature Public Lecture Series

The goal of the series is to promote interdisciplinary inquiry about the environment and to foster dialogue across the campus and community, based on the premise that big questions need interdisciplinary answers.

How are conceptions of nature expressed in the sciences, literature, art, philosophy, music and other disciplines? How did ideas of nature change from 1660 to the present, a period of radical change and revolution?

These lectures are free and open to the public. Due to Covid-19 all lectures will be held through Zoom, please email [email protected] to register. A Zoom link will be sent to you prior …

Anth 110: Introduction To Anthropology: A Peer Review Of Teaching Benchmark Memo, Taylor A. Livingston

UNL Faculty Course Portfolios

This portfolio examines the extent to which the learning outcomes for Introduction to Anthropology (ANTH 110) were met through course assessments. The primary goal of this course is for students to engage with anthropological content, by introducing students to an anthropological perspective, which requires looking at issues from multiple contexts (holism), and having students add this to their “tool kit” to help them make sense of what they believe, why they believe it, and how to make sense of the world around them and their place in it. The alignment of outcomes and assessment was analyzed through an examination of …

Rain Culture : Rain Induced Behavioral Changes Of People Moving On The Streets In Central Ho Chi Minh City, Tuan Anh Luu

Journal of Urban Culture Research

Rain is a natural phenomenon that has existed for a long time in people’s lives, greatly affecting the lifestyle of people and the appearance of Ho Chi Minh City. All the methods, concepts, behaviors and relationships of people in Ho Chi Minh City in their interaction with rain form the whole of the so-called rain induced behavioral culture. Studying the rain induced behavioral changes of people moving on the streets in central Ho Chi Minh City is a meaningful case study to identify the overall picture of behavioral culture with rain in particular, with weather in general, of people in …

Impacts Of Extreme Environmental Disturbances On Piping Plover Survival Are Partially Moderated By Migratory Connectivity, Kristen S. Ellis, Michael J. Anteau, Francesca J. Cuthbert, Cheri L. Gratto-Trevor, Joel G. Jorgensen, David J. Newstead, Larkin Powell, Megan M. Ring, Mark H. Sherfy, Rose J. Swift, Dustin L. Toy, David N. Koons

Nebraska Cooperative Fish and Wildlife Research Unit: Staff Publications

Effective conservation for listed migratory species requires an understanding of how drivers of population decline vary spatially and temporally, as well as knowledge of range-wide connectivity between breeding and nonbreeding areas. Environmental conditions distant from breeding areas can have lasting effects on the demography of migratory species, yet these consequences are often the least understood. Our objectives were to 1) evaluate associations between survival and extreme environmental disturbances at nonbreeding areas, including hurricanes, harmful algal blooms, and oil spills, and 2) estimate migratory connectivity between breeding and nonbreeding areas of midcontinental piping plovers (Charadrius melodus). We used capture …

Place, Self, Community: City As Text™ In The Twenty-First Century, Bernice Braid, Sara Quay

National Collegiate Honors Council Monographs

Acknowledgments

Introduction — Place, Self, Community: City as Text™ in the Twenty-First Century by Bernice Braid

PART 1: Theory and Practice of City as Text™ — Brain Activity and Experiential Learning by Paul Witkovsky • Lost in Learning: Mapping the Position of Teacher in the Classroom and Beyond by Susan M. Cannata, Jesse Peters, Alix Dowling Fink, Edward L. Kinman, JoEllen Pederson, Phillip L. Poplin, and Jessi B. Znosko • Learning from the Land: Creating Authentic Experience-Based Learning that Fosters Sustained Civic Engagement by Ted Martinez and Kevin Gustafson • Integrating Dynamic Systems Theory and City as …

Brain Activity And Experiential Learning, Paul Witkovsky

National Collegiate Honors Council Monographs: Chapters

The chapters in this book deal primarily with students’ learning experiences as documented through self-awareness, knowledge acquisition, and behavior. Language makes it possible to communicate these changes to others. This essay, in contrast, will examine learning from the perspective of brain function. The current framework of thinking among neuroscientists, psychologists, and philosophers is that the brain is fully responsible for our minds, and thus studying how the brain functions in molecular, cellular, and systems terms sheds light on all mental processes, including those that are the substrate of learning. A scientific understanding of brain function thus helps to explain the …