Digital Commons Network^™

The Role Of Non-Specific Interactions In Canonical And Alt-Associated Pml-Bodies Formation And Dynamics, Alexander V. Fonin, Sergey A. Silonov, Olesya G. Shpironok, Iuliia A. Antifeeva, Alexey V. Petukhov, Anna E. Romanovich, Irina M. Kuznetsova, Vladimir N. Uversky, Konstantin K. Turoverov

Molecular Medicine Faculty Publications

In this work, we put forward a hypothesis about the decisive role of multivalent nonspecific interactions in the early stages of PML body formation. Our analysis of the PML isoform sequences showed that some of the PML isoforms, primarily PML-II, are prone to phase separation due to their polyampholytic properties and the disordered structure of their C-terminal domains. The similarity of the charge properties of the C-terminal domains of PML-II and PML-VI isoforms made it possible for the first time to detect migration of PML-VI from PML bodies to the periphery of the cell nucleus, similar to the migration of …

Isolation And Characterization Of Human Colon Adenocarcinoma Stem-Like Cells Based On The Endogenous Expression Of The Stem Markers, Sergei A. Koshkin, Olga V. Anatskaya, Alexander E. Vinogradov, Vladimir N. Uversky, Guy W. Dayhoff Ii, Margarita A. Bystriakova, Valery A. Pospelov, Elena N. Tolkunova

Molecular Medicine Faculty Publications

Background: Cancer stem cells’ (CSCs) self-maintenance is regulated via the pluripotency pathways promoting the most aggressive tumor phenotype. This study aimed to use the activity of these pathways for the CSCs’ subpopulation enrichment and separating cells characterized by the OCT4 and SOX2 expression. Methods: To select and analyze CSCs, we used the SORE6x lentiviral reporter plasmid for viral transduction of colon adenocarcinoma cells. Additionally, we assessed cell chemoresistance, clonogenic, invasive and migratory activity and the data of mRNA-seq and intrinsic disorder predisposition protein analysis (IDPPA). Results: We obtained the line of CSC-like cells selected on the basis of the expression …

The Participation Of The Intrinsically Disordered Regions Of The Bhlh-Pas Transcription Factors In Disease Development, Marta Kolonko-Adamska, Vladimir N. Uversky, Beata Greb-Markiewicz

Molecular Medicine Faculty Publications

The basic helix–loop–helix/Per-ARNT-SIM (bHLH-PAS) proteins are a family of transcription factors regulating expression of a wide range of genes involved in different functions, ranging from differentiation and development control by oxygen and toxins sensing to circadian clock setting. In addition to the well-preserved DNA-binding bHLH and PAS domains, bHLH-PAS proteins contain long intrinsically disordered C-terminal regions, responsible for regulation of their activity. Our aim was to analyze the potential connection between disordered regions of the bHLH-PAS transcription factors, post-transcriptional modifications and liquid-liquid phase separation, in the context of disease-associated missense mutations. Highly flexible disordered regions, enriched in short motives which …

Potential Molecular Mechanisms Of Rare Anti-Tumor Immune Response By Sars-Cov-2 In Isolated Cases Of Lymphomas, Debmalya Barh, Sandeep Tiwary, Lucas Gabriel Rodrigues Gomes, Marianna E. Weener, Khalid J. Alzahrani, Khalaf F. Alsharif, Alaa A. A. Aljabali, Murtaza M. Tambuwala, Kenneth Lundstrom, Sk. Sarif Hassan, Ángel Serrano-Aroca, Kazuo Takayama, Preetam Ghosh, Elrashdy M. Redwan, Bruno Silva Andrade, Siomar De Castro Soares, Vasco Azevedo, Vladimir N. Uversky

Molecular Medicine Faculty Publications

Recently, two cases of complete remission of classical Hodgkin lymphoma (cHL) and follicular lymphoma (FL) after SARS-CoV-2 infection were reported. However, the precise molecular mechanism of this rare event is yet to be understood. Here, we hypothesize a potential anti-tumor immune response of SARS-CoV-2 and based on a computational approach show that: (i) SARS-CoV-2 Spike-RBD may bind to the extracellular domains of CD15, CD27, CD45, and CD152 receptors of cHL or FL and may directly inhibit cell proliferation. (ii) Alternately, upon internalization after binding to these CD molecules, the SARS-CoV-2 membrane (M) protein and ORF3a may bind to gamma-tubulin complex …

Disphscan: A Multi-Sequence Web Tool For Predicting Protein Disorder As A Function Of Ph, Carlos Pintado-Grima, Valentín Iglesias, Jaime Santos, Vladimir N. Uversky, Salvador Ventura

Molecular Medicine Faculty Publications

Proteins are exposed to fluctuating environmental conditions in their cellular context and during their biotechnological production. Disordered regions are susceptible to these fluctuations and may experience solvent-dependent conformational switches that affect their local dynamism and activity. In a recent study, we modeled the influence of pH in the conformational state of IDPs by exploiting a charge–hydrophobicity diagram that considered the effect of solution pH on both variables. However, it was not possible to predict context-dependent transitions for multiple sequences, precluding proteome-wide analysis or the screening of collections of mutants. In this article, we present DispHScan, the first computational tool dedicated …

On The Safety Of The Covid-19 Convalescent Plasma Treatment: Thrombotic And Thromboembolic Concerns, Fatma Elrashdy, Elrashdy M. Redwan, Vladimir N. Uversky

Molecular Medicine Faculty Publications

Recently, it was reported that near-sourced COVID-19 convalescent plasma (CP) is more efficient than distantly sourced CP. What was left behind in this analysis is the investigation of the possible causes of mortality associated with the CP transfusion itself. Knowing this information is important for determining whether not receiving CP of near source is the main cause of high rate of death in the group of patients who received distantly sourced CP. We argue that the thrombotic and thromboembolic events may act as risk factors for adverse complications and death associated with CP transfusion. Therefore, they have to be considered …

Hydrogen Bond Arrangement Is Shown To Differ In Coexisting Phases Of Aqueous Two-Phase Systems, Pedro P. Madeira, Amber R. Titus, Luisa A. Ferreira, Alexander I. Belgovskiy, Elizabeth K. Mann, Jay Adin Mann Jr., William V. Meyer, Anthony E. Smart, Vladimir N. Uversky, Boris Zaslavsky

Molecular Medicine Faculty Publications

Analysis by attenuated total reflection–Fourier transform infrared spectroscopy shows that each coexisting phase in aqueous two-phase systems has a different arrangement of hydrogen bonds. Specific arrangements vary for systems formed by different solutes. The hydrogen bond arrangement is shown to correlate with differences in hydrophobic and electrostatic properties of the different phases of five specific systems, four formed by two polymers and one by a single polymer and salt. The results presented here suggest that the arrangement of hydrogen bonds may be an important factor in phase separation.

Urgent Need For Field Surveys Of Coronaviruses In Southeast Asia To Understand The Sars-Cov-2 Phylogeny And Risk Assessment For Future Outbreaks, Murat Seyran, Sk. Sarif Hassan, Vladimir N. Uversky, Pabitra Pal Choudhury, Bruce D. Uhal, Kenneth Lundstrom, Diksha Attrish, Nima Rezaei, Alaa A. A. Aljabali, Shinjini Ghosh, Damiano Pizzol, Parise Adadi, Tarek Mohamed Abd El-Aziz, Ramesh Kandimalla, Murtaza M. Tambuwala, Amos Lal, Gajendra Kumar Azad, Samendra P. Sherchan, Wagner Baetas-Da-Cruz, Giorgio Palù, Adam M. Brufsky

All publications

No abstract provided.

The Highly Flexible Disordered Regions Of The Sars-Cov-2 Nucleocapsid N Protein Within The 1–248 Residue Construct: Sequence-Specific Resonance Assignments Through Nmr, Marco Schiavina, Letizia Pontoriero, Vladimir N. Uversky, Isabella C. Felli, Roberta Pierattelli

All publications

The nucleocapsid protein N from SARS-CoV-2 is one of the most highly expressed proteins by the virus and plays a number of important roles in the transcription and assembly of the virion within the infected host cell. It is expected to be characterized by a highly dynamic and heterogeneous structure as can be inferred by bioinformatics analyses as well as from the data available for the homologous protein from SARS-CoV. The two globular domains of the protein (NTD and CTD) have been investigated while no high-resolution information is available yet for the flexible regions of the protein. We focus here …

Stable Isotope Analysis Of An Invasive Crab Species, Charybdis Hellerii, In The Indian River Lagoon, Justin R. Meyer

Honors Undergraduate Theses

The world has never been more connected than it is today. While this is true for people, it is also true for Earth's flora and fauna. Unfortunately, this connectedness has contributed to unprecedented invasive species introductions around the world. Most introductions result in an introduced species dying out in the newly invaded territory and never becoming established. Other introduced species establish and persist for years, but never have a noticeable effect on local ecosystems. However, occasionally, an invasive species gets introduced to a new area and has negative impacts on native plant and animal life. The Indo-Pacific swimming crab, Charybdis …

The Psychological Benefits Of A Remote Physical Activity Intervention In Young Adults With Autism Spectrum Disorder, Madisyn T. Pelchat

Honors Undergraduate Theses

Previous studies found that individuals with Autism Spectrum Disorder (ASD) tend to demonstrate poor health behaviors, including unhealthy nutrition habits and low physical activity (PA) levels. Growing research suggests the PA can improve ASD-related symptoms and attenuate the mental and physical adverse effects of the COVID-19 pandemic. Unfortunately, the stay-at-home order was put in place because of COVID-19, which negatively affected health behaviors in many individuals with ASD, including an increase in sedentary behavior and screen time and less PA participation. Prior research also has found that young adults with ASD are at a higher risk for obesity than their …

The Fighting Journey Of A Premature Baby: A Systemic Review Of Developmental And Neurological Complications Of The Premature Baby, Dana Patel

Honors Undergraduate Theses

Prematurity is a worldwide problem. Every year, 15 million babies are born prematurely, and 1 million of those babies die because of related complications. The surviving premature babies are struggling to hold on to their lives, and even when they do live, most of them end up having various complications to survive and get stronger. There are physical complications faced on their journey such as having underdeveloped lungs, pneumonia, obesity, sepsis, retinopathy of prematurity, respiratory distress syndrome, bronchopulmonary dysplasia, asthma, wheezing, bronchiolitis, cerebral palsy, and motor impairment. They can also develop mental and behavioral health complications such as depression, seizures …

Playing Music As A Nursing Intervention To Reduce Distress In Neonatal And Pediatric Acute Care Patients: A Literature Review, Seren E. Özoğlu

Honors Undergraduate Theses

Pediatric and neonatal patients are especially vulnerable to suffering from distress. This literature review identifies research which applies passive music listening to distressed neonatal and pediatric acute care patients. Databases searched to find relevant studies include CINAHL plus with full text, MEDLINE, Alt HealthWatch, APA PsycArticles, and APA PsycInfo from EBSCOhost. Six studies were identified to meet search criteria. The studies that supported music listening with the neonatal and pediatric populations had a positive effect in reducing distress levels. Additional research is warranted to further validate these findings. Music listening with neonatal and pediatric patients is a simple, cost-effective intervention …

Environmental Monitoring Report: For Work Performed: July 1, 2020 Through June 30, 2021, Tennessee. Department Of Environment And Conservation

Environmental Monitoring Reports

This FY2021 EMR presents results for 20 independent projects, originally defined in TDEC’s FY2021 Environmental Monitoring Plan (EMP) and subsequently completed over the course of FY2021 period of performance.

Concerning Measures To Improve Energy Efficiency, And, In Connection Therewith, Requiring Owners Of Large Buildings To Collect And Report On Energy-Use Benchmarking Data And Comply With Rules Regarding Performance Standards Related To Energy And Greenhouse Gas Emissions And Modifying Statutory Requirements Regarding Energy Performance Contracts., Colorado General Assembly

Session Laws 2001-Present

No abstract provided.

Concerning The Protection Of A Pregnant Person’S Rights During The Perinatal Period, And, In Connection Therewith, Making An Appropriation., Colorado General Assembly

Session Laws 2001-Present

No abstract provided.

Concerning Measures To Increase Access To Prescription Insulin For Persons With Diabetes., Colorado General Assembly

Session Laws 2001-Present

No abstract provided.

News On Social Media, Rwa, And Anti-Asian Sentiment During Covid-19 Pandemic, Hang Duong

Honors Undergraduate Theses

This study examined the relationship between exposure to COVID-19 fake news, right-wing authoritarianism (RWA), sociodemographic factors (i.e., race, biological sex), and xenophobia along with anti-Asian sentiment during the pandemic. Participants included 133 female and male college students. Participants were randomly assigned to one of three conditions: (1) exposed to COVID-19 fake news, (2) exposed to real news related to COVID-19 news, and (3) no news exposure. All participants from three groups were then asked to complete a series of measures regarding their attitudes toward Asian Americans and xenophobia. Participants levels of RWA and sociodemographic variables of race and biological sex …

Concerning The Continuation Of The State Board Of Pharmacy, And, In Connection Therewith, Implementing Recommendations Contained In The 2020 Sunset Report By The Department Of Regulatory Agencies And Making Other Changes Regarding The Practice Of Professions Regulated By The Board., Colorado General Assembly

Session Laws 2001-Present

No abstract provided.

Reduced Mitochondrial Dna And Oxphos Protein Content In Skeletal Muscle Of Children With Cerebral Palsy, Ferdinand Von Walden, Ivan J. Vechetti Jr, Davis Englund, Vandre C. Figueiredo, Rodrigo Fernandez-Gonzalo, Kevin Murach, Jessica Pingel, John J. Mccarthy, Per Stal, Eva Ponten

Department of Nutrition and Health Sciences: Faculty Publications

AIM To provide a detailed gene and protein expression analysis related to mitochondrial biogenesis and assess mitochondrial content in skeletal muscle of children with cerebral palsy (CP). METHOD Biceps brachii muscle samples were collected from 19 children with CP (mean [SD] age 15y 4mo [2y 6mo], range 9–18y, 16 males, three females) and 10 typically developing comparison children (mean [SD] age 15y [4y], range 7–21y, eight males, two females). Gene expression (quantitative reverse transcription polymerase chain reaction [PCR]), mitochondrial DNA (mtDNA) to genomic DNA ratio (quantitative PCR), and protein abundance (western blotting) were analyzed. Microarray data sets (CP/aging/bed rest) were …

I Have A Story To Tell: Will You Listen?, Amy L. Belisle

International Journal of Servant-Leadership

Amy L Belisle is currently enrolled in the Doctor of Leadership Studies program at Gonzaga University and is a 2014 graduate of Gonzaga University’s Master of Organizational Leadership program. Prior to earning her Master from Gonzaga University, she completed a Bachelor of Arts in Biology at Franklin College, in Franklin, Indiana and a Bachelor of Science in Leadership Studies at Providence College, in Providence Rhode Island. She has been living and working in Rhode Island, for Amgen, Inc., for the last 18 years. Her current role is in the Product Quality organization as a Senior Manager. In addition to her …

Seed-Shattering Phenology At Soybean Harvest Of Economically Important Weeds In Multiple Regions Of The United States. Part 2: Grass Species, Lauren M. Schwartz-Lazaro, Lovreet S. Shergill, Jeffrey A. Evans, Muthukumar V. Bagavathiannan, Shawn C. Beam, Mandy D. Bish, Jason A. Bond, Kevin W. Bradley, William S. Curran, Adam S. Davis, Wesley J. Everman, Michael L. Flessner, Steven C. Haring, Nicholas R. Jordan, Nicholas E. Korres, John L. Lindquist, Jason K. Norsworthy, Tameka L. Sanders, Larry E. Steckel, Mark J. Vangessel, Blake Young, Steven B. Mirsky

Department of Agronomy and Horticulture: Faculty Publications

Seed shatter is an important weediness trait on which the efficacy of harvest weed seed control (HWSC) depends. The level of seed shatter in a species is likely influenced by agroecological and environmental factors. In 2016 and 2017, we assessed seed shatter of eight economically important grass weed species in soybean [Glycine max (L.) Merr.] from crop physiological maturity to 4 wk after maturity at multiple sites spread across 11 states in the southern, northern, and mid-Atlantic United States. From soybean maturity to 4 wk after maturity, cumulative percent seed shatter was lowest in the southern U.S. regions and increased …

Coal Char Effects On Soil Chemical Properties And Maize Yields In Semi-Arid Region, Dinesh Panday, Maysoon M. Mikha, Xiaocun Sun, Bijesh Maharjan

Department of Agronomy and Horticulture: Faculty Publications

Soil amendments with high carbon (C) content can be effective in semi-arid regions where soils are characterized by low C. A field study was conducted in 2016–2018 to evaluate the effect of char on soil chemical properties and irrigated maize (Zea mays L.) yields in sandy loam fertilized with urea or composted manure. Carbon-rich char used was a product of coal combustion residue from a local factory in western Nebraska. The experiment was arranged in a split-plot randomized complete block design in four replications with char (0, 6.7, 13.4, 20.1, and 26.8Mg C ha⁻¹) as main and …

A Rare Case Report Of A Corpus Callosal Splenial Lesion In The Context Of Atypical Neuroleptic Malignant Syndrome., Ryan Serdenes, Samuel Orr, Pamela Trio, Seetha Chandrasekhara, Meghan Musselman

Transitional Year Resident Research

In this report, we describe a case of atypical neuroleptic malignant syndrome (NMS) presenting with an isolated lesion in the splenium of the corpus callosum (ILSCC). There is a paucity of information regarding this topic within the literature and only 7 previous case reports have been published at the time of writing. To our knowledge, this case report is also the first to describe an atypical NMS variant in the context of an ILSCC. In this report, we describe the important considerations in formulating differential diagnosis for ILSCC and are the first report to propose a possible pathophysiological mechanism relating …

2020-2021 Graduate Survey Report, Shefei Schultz

Institutional Effectiveness & Planning Documents

Contents:

Graduate College Staff --- 2
Degrees Awarded 2020-2021 --- 3
Graduate Demographics --- 4
Degrees Awarded over 5 Years --- 5
Survey Methodology --- 7
Post-Graduation Activity --- 8
Activity During UNI Experience --- 9
Reasons to Attend UNI --- 11
Confidence in Graduate Experience --- 12
Graduate Education Finance --- 14
Survey Sample --- 18

Understanding Competency Based Learning For Scientific Literacy, Sri Aurobindo Society, Australian Council For Educational Research (Acer)

ACER India

Competency-based learning, unlike curriculum-based learning, emphasizes a student-centred approach to education. It is heavily driven by experience and discovery rather than lecture and memorization. The focus of competency-based learning is on what students understand and how they apply what they learn in real-life situations rather than what they remember. This learning framework for scientific literacy is an effort to introduce competency-based learning to classroom teaching and learning in India to prepare Indian students for the rapidly changing future. It provides resources that can be used by schools and teachers for supporting secondary school students to acquire scientific literacy and problem-solving …

Voiced Gender Signifiers Within ‘As One’ A Chamber Opera: Annotated Bibliography, Samuel Sherman

Musicology and Ethnomusicology: Student Scholarship

‘As One’, a popular modern chamber opera by composer Laura Kaminsky, presents the transition of transgender woman, Hannah. Hannah, however, is casted and composed as two separate voices, a baritone and mezzo-soprano, thus creating “Hannah before” and “Hannah after”. In doing so, a dichotomy of thought surrounding the attributes of a binary based practice of vocalization and character representation is created. Through the analysis of gender theory, voiced gender theory, and the ‘As One’ chamber opera, storytelling of complex gender-based characters may be solidified within biased gender compositional and musical tools. In discovering these repetitive actions through socially constructed stereotypes …

A Blockchain-Enabled Model To Enhance Disaster Aids Network Resilience, Farinaz Sabz Ali Pour, Paul Niculescu-Mizil Gheorghe

Engineering Management & Systems Engineering Faculty Publications

The disaster area is a true dynamic environment. Lack of accurate information from the affected area create several challenges in distributing the supplies. The success of a disaster response network is based on collaboration, coordination, sovereignty, and equality in relief distribution. Therefore, a trust-based dynamic communication system is required to facilitate the interactions, enhance the knowledge for the relief operation, prioritize, and coordinate the goods distribution. One of the promising innovative technologies is blockchain technology which enables transparent, secure, and real-time information exchange and automation through smart contracts in a distributed technological ecosystem. This study aims to analyze the application …

Ndikimi I Sulmeve Kibernetike Në Sektorin Bankar Dhe Shëndetësor Gjatë Vitit 2020, Triflona Tolaj

Theses and Dissertations

- Punimi im i temës së diplomës është një hulumtim shkencor rreth sulmeve kibernetike dhe ndikimit të tyre në dy sektorët më të rrezikuar, sektorin bankar dhe atë shëndetësor. Tema është përzgjedhur e tillë pasi që çdo ditë përballemi me risi të reja në fushën e teknologjisë. Kjo gjë ka ndikuar edhe në rritjen e sulmeve kibernetike, e numri i tyre është shtuar edhe më shumë gjatë periudhës së po këtij viti, pandemisë së koronavirusit. Në kapitujt e parë të projektit është shtjelluar fillimisht koncepti i sigurisë kibernetike dhe masat që duhet të merren për mbrojtjen ndaj sulmeve të mundshme, …

Kujdesi Infermieror Te Pacientët Me Artrit Reumatoid, Valbona Zeqiraj

Theses and Dissertations

Artriti reumatoid është një çrregullim inflamator, sistemik,kronik i cili kryesisht prek nyjet që janë të veshura me synovium. Karakterizohet tipikisht nga një përfshirje simetrike,herë pas here deformuar, poliartriti periferik. Për shkak se është një sëmundje sistemike, mundet gjithashtu ndikojnë në të gjithë trupin, duke përfshirë zemrën, mushkëritë dhe sytë.

Shkaku AR nuk njihet plotësisht. Ka të ngjarë që individët e ndjeshëm gjenetikisht të ekspozohen ndaj një antigjeni të panjohurduke rezultuar në vetë-stimulim të sistemit imunitar (autoimuniteti).

Përgjigja imune reagon në mënyrë të kryqëzuar me indin pritës (membranën synoviale) duke rezultuar në pezmatim të membranës sinoviale (sinovit).Kjo krijon hipertrofi sinoviale.

Ky …