Digital Commons Network^™

Navigating Glucosinolate Biosynthesis And High Oleic Acid Seed Oil Production In Pennycress ( Thlaspi Arvense L. ) Utilizing Crispr / Cas9 Genome Editing, Brice Jarvis

Theses and Dissertations

Pennycress (Thlaspi arvense L.) is being domesticated as an oilseed cash cover crop to be grown in the offseason throughout temperate regions of the world. With its diploid genome and ease of molecular mutagenesis, pennycress seed oil composition can be rapidly tailored for a plethora of food, feed, oleochemical and fuel uses. The first part of my research has utilized Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/Cas9 technology to produce knockout mutations throughout the lipid biosynthetic pathway, with an emphasis to increase oleic acid (C18:1) content specifically within the seed. Oleic acid (18:1) is valued for its oxidative stability that …

Microrna Heterogeneity, Innate-Immune Defense And The Efficacy Of Sars-Cov-2 Infection-A Commentary, Walter J. Lukiw

School of Graduate Studies Faculty Publications

Severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2), a member of the genus Betacoronavirus in the family Coronaviridae, possesses an unusually large single-stranded viral RNA (ssvRNA) genome of about ~29,811 nucleotides (nt) that causes severe and acute respiratory distress and a highly lethal viral pneumonia known as COVID-19. COVID-19 also presents with multiple ancillary systemic diseases and often involves cardiovascular, inflammatory, and/or neurological complications. Pathological viral genomes consisting of ssvRNA, like cellular messenger RNA (mRNA), are susceptible to attack, destruction, neutralization, and/or modulation by naturally occurring small non-coding RNAs (sncRNAs) within the host cell, some of which are known as microRNAs (miRNAs). …

The Chancellors Are Alright: Nationwide Injunctions And An Abstention Doctrine To Salve What Ails Us, Ezra Ishmael Young

Cleveland State Law Review

This Article endeavors to reclaim the nationwide injunction as a valid exercise of federal equity power within the jurisdictional limits set by Article III. It posits that federal equity is expansive—it extends as far as necessary to provide a remedy where there is no adequate one at law. Historical and doctrinal context and critique are deployed to demonstrate that nationwide injunctions are not constitutionally ultra vires. This Article also posits that despite having expansive equity jurisdiction and powers, federal courts can and should in many cases exercise their constitutional discretion when sitting in equity to abstain in certain nationwide injunction …

A Fret Flow Cytometry-Based Screening Assay For Multiplex Analysis Of Metabolites In T. Brucei, Ronald A. Zegarra

Undergraduate Honors Theses

Kinetoplastid parasites are a significant public health issue in some tropical and subtropical regions of the world. Kinetoplastid parasites all require glycolysis for survival, with host glucose key for ATP production. One such parasite, Trypanosoma brucei, exclusively metabolizes glucose in its bloodstream form. Trypanosomal glycolysis is unique because it displays unconventional structural features. Hence, glucose metabolism has been studied extensively in T. brucei and is a therapeutic target in kinetoplastid parasites.The lack of in vivo analytical techniques for measuring vital glycolytic metabolites in situ has restricted the ability of researchers to test, with high sensitivity and specificity, the essential roles …

Sweet Sorghum Genotypes Tolerant And Sensitive To Nitrogen Stress Select Distinct Root Endosphere And Rhizosphere Bacterial Communities, Lucas Dantas Lopes, Yen Ning Chai, Ellen L. Marsh, John F. Rajewski, Ismail Dweikat, Daniel P. Schachtman

Department of Agronomy and Horticulture: Faculty Publications

The belowground microbiomes have many beneficial functions that assist plant growth, including nutrient cycling, acquisition and transport, as well as alleviation of stresses caused by nutrient limitations such as nitrogen (N). Here we analyzed the root endosphere, rhizosphere and soil bacterial communities of seven sweet sorghum genotypes differing in sensitivity to N-stress. Sorghum genotypes were grown in fields with no (low-N) or sufficient (high-N) N. The dry shoot weight ratio (low-N/high-N) was used to determine N-stress sensitivity. Our hypothesis was that genotypes tolerant and sensitive to N-stress select distinct bacterial communities. The endosphere and rhizosphere bacterial community structure were significantly …

The Early Medieval Transition: Diet Reconstruction, Mobility, And Culture Contact In The Ravenna Countryside, Northern Italy, Anastasia Temkina

USF Tampa Graduate Theses and Dissertations

This research project evaluates the effects of increased mobility and culture contact on dietary practices, and on dietary variation among people buried at two northern Italian sites, Chiunsano di Ficarolo and Chiesazza di Ficarolo, located near the ancient Roman capital of Ravenna and dating 4th to 7th century CE. The Early Medieval period was a time of change, political instability, migration and invasion of the “barbarian” tribes, and diet was not unaffected. In particular, it is hypothesized that a new staple crop, millet, was introduced and that meat consumption had increased. The goal of this research is to use stable …

June 17-18, 2021, Ohio University Board Of Trustees Meeting Minutes, Ohio University Board Of Trustees

Board of Trustees Meeting Minutes

Meeting minutes document the activities of Ohio University's Board of Trustees.

Atypical Presentation Of Esophageal Squamous Cell Carcinoma Masquerading As Diffuse Severe Esophagitis, Hafsa Abbas, Haider Ghazanfar, Ali Naqqi Ul Hussain, Ahmed Baiomi, Ariyo Ihimoyan

Publications and Research

Esophagitis is described as an inflammation of the esophagus and can result from multiple etiologies. Esophageal squamous cell carcinoma (ESCC), presenting as diffuse esophagitis in the absence of a mass or lesion, is rare. We present a case of a 61-year-old man who presented to the gastroenterology clinic for dysphagia and heartburn of 3 months duration. The patient had lost about 15 pounds unintentionally over 6 months. The patient underwent esophagogastroduodenoscopy, which revealed significant diffuse Grade 4 esophagitis without any overt bleeding. Random biopsies were taken with cold forceps from proximal, middle, and distal esophageal segments because of the striking …

Structurally Distinct External Solvent-Exposed Domains Drive Replication Of Major Human Prions, Mohammad Khursheed Siddiqi, Chae Kim, Tracy Haldiman, Miroslava Kacirova, Benlian Wang, Jen Bohon, Mark R. Chance, Janna G. Kiselar, Jiri G. Safar

Faculty Scholarship

There is a limited understanding of structural attributes that encode the iatrogenic transmissibility and various phenotypes of prions causing the most common human prion disease, sporadic Creutzfeldt-Jakob disease (sCJD). Here we report the detailed structural differences between major sCJD MM1, MM2, and VV2 prions determined with two complementary synchrotron hydroxyl radical footprinting techniques—mass spectrometry (MS) and conformation dependent immunoassay (CDI) with a panel of Europium-labeled antibodies. Both approaches clearly demonstrate that the phenotypically distant prions differ in a major way with regard to their structural organization, and synchrotron-generated hydroxyl radicals progressively inhibit their seeding potency in a strain and structure-specific …

Determining Predictors Of Peer Relations: A Study On Youth Inethiopia, India, Peru, And Vietnam, Susanna Fullmer

Theses and Dissertations

Across the world countries are currently striving to eliminate poverty, improve the quality of education, optimize well-being, among other areas of improvement. In an effort to help such improvements, a group called Young Lives ran a longitudinal study on youth in Ethiopia, India, Peru, and Vietnam that studied the many facets of poverty. The purpose of this study is to utilize the Young Lives dataset to determine how countries can more readily improve social-emotional skills by looking at important experiences in adolescents' lives. Specifically, this study examines what factors increase a child's ability to socialize with peers, which is shown …

Design Of A Pneumatic Soft Robotic Actuator Using Model-Based Optimization, Mahsa Raeisinezhad

Theses and Dissertations

In this thesis, the design and optimization process of a novel soft intelligent modular pad (IntelliPad) for the purpose of pressure injury prevention is presented. The structure of the IntelliPad consists of multiple individual multi-chamber soft pneumatic-driven actuators that use pressurized air and vacuum. Each actuator is able to provide both vertical and horizontal motions that can be controlled independently. An analytical modeling approach using multiple cantilever beams and virtual springs connected in a closed formed structure was developed to analyze the mechanical performance of the actuator. The analytical approach was validated by a finite element analysis. For optimizing the …

Associations Between Community Built Environments With Early Care And Education Classroom Physical Activity Practices And Barriers, Bethany D. Williams, Susan B. Sisson, Dipti Dev, Bryce Lowery, Diane Horm, Janis Campbell, Denise Finneran, Jennifer Graef-Downard, Linda Whaley

Department of Child, Youth, and Family Studies: Faculty Publications

The influence of community-built environments on physical activity (PA) support in Early Childhood Education settings (ECEs) is unknown. The purpose of this cross-sectional study was to determine associations between community PA environments and ECE classroom PA practices. We included licensed Oklahoma ECE directors serving 3-to-5-year-old children. Parks and playground locations were exported from Google Earth. National Walkability Index was derived from 2010 US Census data. ArcMap 10.6 was used to geocode ECE locations, which were within an Activity Desert if no parks/playgrounds were located within a 1-mile radius or if Walkability Index was 10.5 or below. Classroom PA practices were …

Um Researcher Provides The Skinny On Normal Weight Obesity, Sarah Sapp

University of Mississippi News

Nadeeja Wijayatunga publishes study of 'skinny fat' health risks in International Journal of Obesity

Variability In Ckd Biomarker Studies: Soluble Urokinase Plasminogen Activator Receptor (Supar) And Kidney Disease Progression In The Chronic Kidney Disease In Children (Ckid) Study., Alison G. Abraham, Yunwen Xu, Jennifer L. Roem, Jason H. Greenberg, Darcy K. Weidemann, Venkata S. Sabbisetti, Joseph V. Bonventre, Michelle Denburg, Bradley A. Warady, Susan L. Furth

Manuscripts, Articles, Book Chapters and Other Papers

Rationale & Objective: Biomarker studies are important for generating mechanistic insight and providing clinically useful predictors of chronic kidney disease (CKD) progression. However, variability across studies can often muddy the evidence waters. Here we evaluated real-world variability in biomarker studies using two published studies, independently conducted, of the novel plasma marker soluble urokinase-type plasminogen activator receptor (suPAR) for predicting CKD progression in children with CKD.

Study Design: A comparison of 2 prospective cohort studies.

Setting & Participants: 541 children from the Chronic Kidney Disease in Children (CKiD) study, median age 12 years, median glomerular filtration rate (GFR) of 54 mL/min/1.73m …

A Feasibility Study Of A Group-Format, Ecologically Oriented Neurorehabilitation Of Memory (Eon-Mem) Program In Older Adults, Kayla Michelle Frodsham

Theses and Dissertations

Older adults often show declines in subjective and objective memory performance relative to younger adults. One potential path for helping older adults with memory may be compensatory memory training programs. Compensatory memory training programs teach strategies to manage memory impairment. Traditional compensatory memory training programs tend to be highly specific to a task and often do not generalize to other memory tasks. Ecologically Oriented Neurorehabilitation of Memory (EON-Mem) is a method for teaching memory strategies that may generalize for efficient use in everyday contexts. We performed a feasibility study to determine the value of pursuing a group-based version of EON-Mem …

A Pragmatic Machine Learning Model To Predict Carbapenem Resistance, Ryan J Mcguire, Sean C Yu, Philip R O Payne, Albert M Lai, M Cristina Vazquez-Guillamet, Marin H Kollef, Andrew P Michelson

Open Access Publications

Infection caused by carbapenem-resistant (CR) organisms is a rising problem in the United States. While the risk factors for antibiotic resistance are well known, there remains a large need for the early identification of antibiotic-resistant infections. Using machine learning (ML), we sought to develop a prediction model for carbapenem resistance. All patients >18 years of age admitted to a tertiary-care academic medical center between 1 January 2012 and 10 October 2017 with ≥1 bacterial culture were eligible for inclusion. All demographic, medication, vital sign, procedure, laboratory, and culture/sensitivity data were extracted from the electronic health record. Organisms were considered CR …

Cureing Exposure To Environmental Chemicals From Personal Care Products, Erika L. Doctor, Cassandra S. Korte

Faculty and Staff Publications & Presentations

This series of laboratory modules presents students the opportunity to perform in-classroom research while building their working knowledge of the scientific process and growing their skills in analytical methods. In the laboratory, students analyze samples of urine collected as part of an intervention study focused on reducing exposure to potentially harmful compounds, phthalates, found in personal care products. Students propose methods of extraction and detection, analyze samples from the intervention study, analyze data, present results, and propose future iterations of the intervention-study. In this workshop, participants experience the online student experience through the steps of extracting phthalates from urine samples, …

Dietary Fiber Intake, The Gut Microbiome, And Chronic Systemic Inflammation In A Cohort Of Adult Men, Wenjie Ma, Yin Cao, Et Al.

2020-Current year OA Pubs

BACKGROUND: A higher intake of dietary fiber is associated with a decreased risk of chronic inflammatory diseases such as cardiovascular disease and inflammatory bowel disease. This may function in part due to abrogation of chronic systemic inflammation induced by factors such as dysbiotic gut communities. Data regarding the detailed influences of long-term and recent intake of differing dietary fiber sources on the human gut microbiome are lacking.

METHODS: In a cohort of 307 generally healthy men, we examined gut microbiomes, profiled by shotgun metagenomic and metatranscriptomic sequencing, and long-term and recent dietary fiber intake in relation to plasma levels of …

Resistance Training As Therapeutic Management In Women With Pcos: What Is The Evidence?, Pamela J. Wright, Cynthia F. Corbett, Bernardine M. Pinto, Robin M. Dawson, Michael Wirth

International Journal of Exercise Science

International Journal of Exercise Science 14(3): 840-854, 2021. Polycystic ovary syndrome (PCOS), the most common chronic endocrinopathy and the leading cause of infertility in women, has significant clinical consequences, including cardiovascular, endocrinological, oncological, and psychological co-morbidities. Endocrine Society Clinical Practice Guidelines on the Diagnosis and Treatment of PCOS recommend exercise and physical activity as first-line treatment to combat chronic disease risk. However, details about what type of exercise are not provided. Given the known beneficial effects of resistance training on the management of other chronic diseases, the purpose of this scoping review was to evaluate the scientific evidence about the …

We Are...Marshall, June 16, 2021, Office Of Marshall University Communications

We Are ... Marshall: the Newsletter for Marshall University 1999-2023

No abstract provided.

Upjohn Press Catalog 2021-2022, W.E. Upjohn Institute For Employment Research

Upjohn Press

No abstract provided.

Exploring Community Health Worker Roles, Support, And Experiences In The Context Of The Covid-19 Pandemic In Bangladesh, Frontline Health Project

Reproductive Health

This brief presents results from the Frontline Health project's study in Bangladesh which explores CHWs' perspectives and challenges providing support to their communities during COVID-19.

Novel Disk Diffusion Assay On Magnesium Oxalate Agar To Evaluate The Susceptibility Of Yersinia Pestis To Type Iii Secretion System Inhibitors, Sukriti Prashar, Miguel Portales Guemes, Poorandai Shivbaran, Eugenia Jimenez Alvarez, Christopher Soha, Samir Nacer, Michael Mcdonough, Gregory V. Plano, Julie Torruellas Garcia

Biology Faculty Articles

Current methods for screening small molecules that inhibit the plasmid pCD1-encoded Yersinia pestis type III secretion system (T3SS) include lengthy growth curves followed by multistep luminescence assays or Western blot assays to detect secretion, or lack thereof, of effector proteins. The goal of this research was to develop a novel disk diffusion assay on magnesium oxalate (MOX) agar as a simple way to evaluate the susceptibility of Y. pestis to type III secretion system inhibitors. MOX agar produces distinct Y. pestis growth characteristics based on the bacteria’s ability or inability to secrete effector proteins; small, barely visible colonies are observed …

Implementation Of Student-Centered Teaching Methods Among Stem Faculty, Melissa Cavan

Theses and Dissertations

Students at the college level need good instruction and active learning has been shown to improve student retention and learning. Science, Technology, Engineering, and Math Faculty Institution (STEMFI) aims to assist faculty in implementing active learning in the classroom. This qualitative case study sought to understand faculty perspectives two years after completion of STEMFI in the areas of active learning implementation, methodology and student attitudes and impact. Data collected reported that the faculty participants still use active learning strategies in their classrooms two years after exiting the STEMFI program. The faculty also felt the need for a refresher course and …

Homeless Students' Barriers To Education: Before And After Covid-19, Linda M. Twidwell

University Honors Theses

This thesis is an examination of the barriers to education homeless youth may be faced with both before and after the COVID-19 pandemic. Using past literature, this paper will focus on three main barriers homeless students may have faced before the pandemic. These are homeless students’ lack of support needed to succeed, how federal policy shapes homeless students’ opportunities and rights, and that the rate of children experiencing homelessness is rising, and little is being done about the fact. After discussing the literature before March 2020, this paper will examine the literature that has come out since the pandemic’s beginning, …

Literature Review: Pollutant Removal Efficacy Of Floating Treatment Wetlands Across Water Bodies, Sophie Johnson

University Honors Theses

Wetland numbers are declining worldwide and there is a need to replace the water filtration services they provide. One emerging option is floating treatment wetlands (FTW). FTW are a floating mat that serves as a habitat for aquatic plants whose roots are suspended in the water and that remove both organic and inorganic pollutants like nutrients (nitrogen and phosphorus), potentially toxic metals, and suspended solids. A literature search was performed to examine the efficacy of FTW pollutant removal. Specifically, I inspected 1) how effective FTW are at removing a range of nutrients; 2) what types of plants are most effective …

A Screen For Sleep And Starvation Resistance Identifies A Wake-Promoting Role For The Auxiliary Channel Unc79, Kazuma Murakami, Justin Palermo, Bethany A. Stanhope, Allen G. Gibbs, Alex C. Keene

Life Sciences Faculty Research

The regulation of sleep and metabolism are highly interconnected, and dysregulation of sleep is linked to metabolic diseases that include obesity, diabetes, and heart disease. Furthermore, both acute and long-term changes in diet potently impact sleep duration and quality. To identify novel factors that modulate interactions between sleep and metabolic state, we performed a genetic screen for their roles in regulating sleep duration, starvation resistance, and starvation-dependent modulation of sleep. This screen identified a number of genes with potential roles in regulating sleep, metabolism, or both processes. One such gene encodes the auxiliary ion channel UNC79, which was implicated in …

Preparing Undergraduates For Agriculture And Food Research Careers: An Internship Paradigm, Rebecca Mott, Todd Lorenz, Jo Britt-Rankin

Journal of Extension

The University of Missouri Integrated STEM Internship Program (MU-ISIP) is a 9-week internship experience that helps prepare undergraduates for the workforce or graduate school in four AFRI areas: (a) plant health and production, (b) animal health and production, (c) food safety, nutrition and health, and (d) agricultural economics and rural communities. This program helps address the need for the next generation of agriculturalists and food scientists through experiential learning. Additionally, it exposes undergraduates to Extension as a career option. This article outlines our approach to MU-ISIP and lessons learned during the program’s inception.

Evacuation Decision Making And The Role Of Social Connections Across Evacuation Statuses During Hurricane Irma, Nathan D. Shull

USF Tampa Graduate Theses and Dissertations

The purpose of this research was to examine the evacuation decision-making process during Hurricane Irma in 2017. Special consideration was given to the role of an individual’s social connections (SCs). Additionally, the dependability, density (i.e., social network size and number of connections), and diversity of SCs were compared between those who: (a) evacuated to a shelter in Pinellas County, (b) evacuated to somewhere outside of the Tampa Bay area, and (c) did not evacuate. Data for evacuees and non-evacuees from Collins et al. (2018) were used to perform this comparison, while the data for shelter evacuees were acquired via a …

Exploring Connections Between Food Sovereignty, Cultural Food Access And Urban Health Promotion: A Systematic Review Of The Literature, Stephanie L. Kenyon

University Honors Theses

Marginalized urban communities face disproportionate rates of food insecurity related health outcomes and are more likely to lose important connections to traditional and cultural foods. Food sovereignty approaches, including policy changes, decolonized methods and community created perspectives to research and interventions have the potential to impact access to traditional and cultural foods, improving food security and supporting a healthier diet. The evidence in support of a food sovereignty approach to public health research and practice is limited and much of what is known is primarily based on studies at the global level or on rural communities. This systematic literature review …