Digital Commons Network^™

No Ordinary Proteins: Adsorption And Molecular Orientation Of Monoclonal Antibodies, Ankit D. Kanthe, Andrew Ilott, Mary Krause, Songyan Zheng, Jinjiang Li, Wei Bu, Mrinal K. Bera, Binhua Lin, Charles Maldarelli, Raymond S. Tu

Publications and Research

The interaction of monoclonal antibodies (mAbs) with air/water interfaces plays a crucial role in their overall stability in solution. We aim to understand this behavior using pendant bubble measurements to track the dynamic tension reduction and x-ray reflectivity to obtain the electron density profiles (EDPs) at the surface. Native immunoglobulin G mAb is a rigid molecule with a flat, “Y” shape, and simulated EDPs are obtained by rotating a homology construct at the surface. Comparing simulations with experimental EDPs, we obtain surface orientation probability maps showing mAbs transition from flat-on Y-shape configurations to side-on or end-on configurations with increasing concentration. …

Cfd Analysis Of Subcutaneous Deposition And Dispersion Of Insulin In Adipose Tissue, Ryan C. Lubbers

Masters Theses

Drug delivery is the most important factor of many therapies, but a lack of technology and research have led to a very generalized understanding of drug kinetics. Insulin pump therapy for the treatment of Type 1 Diabetes depends on precise delivery of the hormone into the adipose region between the dermis and underlying muscle. The kinetics of insulin within the adipose tissue environment is not well understood and varies greatly case to case. The use of computation fluid dynamics (CFD) models to study insulin kinetics in relation to influential factors will lead to a better understanding of the characteristics of …

An Out Of This World Experience, Rebecca Irelan

UNH Today Archive

No abstract provided.

Racial And Socioeconomic Disparities Influencing Obesity Amongst Middle-Aged Women, Morgan Woods

Master of Science in Integrative Biology Theses

Many studies have examined the effects of racial and socioeconomic differences on health-related disparities involving obesity but, few have examined the mental and physical well-being intersection. Obesity is a disease that affects many Americans and has become an epidemic affecting the American population. Obesity, especially morbid obesity, is a risk factor for many other obesity-related diseases such as diabetes, cardiovascular diseases, hypertension, and more. Using the 2018 Healthcare Avoidance Study, differences were found between two racial groups, Caucasian and African-American women. Race was found as a significant factor for both level of physical activity and ideal body image perception

The Effectiveness Of Preoperative Preparation Program In Decreasing Parental Preoperative Anxiety, Thao L. Tran

Dissertations

This project aimed to examine the effectiveness of a preoperative preparation program (PPP) in decreasing parental anxiety. When children needed anesthesia to get outpatient procedures completed, the accompanying parents received verbal education about the process on the day of the procedure and were assisted by a support person during and after the child's induction anesthesia. Parental anxiety was measured using the State-Trait Anxiety Inventory (STAI) tool before preoperative preparation and after the induction of anesthesia to assess whether the PPP improved parents' anxiety levels. The parents' decreased anxiety score in post-intervention would suggest that the PPP can be effectively and …

Positron Emission Tomography From 1930 To 1990: The Epistemology And Process Of Scientific Instrumentation, Rick Shang

Arts & Sciences Graduate Student Theses and Dissertations

Positron Emission Tomography (PET) is a groundbreaking detection system that, among many other applications, enabled neuroscience researchers to detect and image physiological changes in the brain associated with cognitive operations as subjects perform a task. This technology helped researchers learn to explore the physiological basis of human cognition in new ways and was the immediate forerunner of functional Magnetic Resonance Imaging (fMRI), a technology that continues to dominate the neurosciences. PET was also the founding invention of a new field of cognitive science: human functional neuroimaging. Indeed, some would say that PET encouraged a particular way of understanding the brain …

Positron Emission Tomography From 1930 To 1990: The Epistemology And Process Of Scientific Instrumentation, Rick Shang

Arts & Sciences Graduate Student Theses and Dissertations

Positron Emission Tomography (PET) is a groundbreaking detection system that, among many other applications, enabled neuroscience researchers to detect and image physiological changes in the brain associated with cognitive operations as subjects perform a task. This technology helped researchers learn to explore the physiological basis of human cognition in new ways and was the immediate forerunner of functional Magnetic Resonance Imaging (fMRI), a technology that continues to dominate the neurosciences. PET was also the founding invention of a new field of cognitive science: human functional neuroimaging. Indeed, some would say that PET encouraged a particular way of understanding the brain …

Volume 32, Numbers 1, 2, 3, 4, Fall 1994/Winter 1995/Spring 1995/Summer 1995 Speaker And Gavel

Speaker & Gavel

Complete digitized volume (volume 32, numbers 1-4, Fall 1994/Winter 1995/Spring 1995/Summer 1995) of Speaker & Gavel.

The Beneficial Effects Of The Gut-Derived Metabolite Trimethylamine N-Oxide On Functional Β-Cell Mass, Emily Suzanne Krueger

Theses and Dissertations

Elevated serum levels of trimethylamine N-oxide (TMAO) were first associated with increased risk of cardiovascular disease (CVD) 10 years ago. Research has since defined that serum TMAO accumulation is controlled by the diet-microbiome-liver-kidney axis. Choline related nutrients are consumed in excess during over-nutrition from a Western diet. The resultant elevated serum TMAO is investigated across various chronic metabolic diseases and many tissue types. While TMAO is most clearly linked to CVD mechanisms in vascular tissue, its molecular effects on metabolic tissues are unclear. Here we report the current standing of TMAO research in metabolic disease context across relevant metabolic tissues …

Puma And The Innate Immune Response During Pneumococcal Infection In The Lung, Daniel Edward Kennedy Ii

Theses and Dissertations

Background: The p53-up-regulated modulator of apoptosis (PUMA) protein is a pro-apoptotic, BH3-only member of the BCL2 family of effector proteins responsible for promoting organized cell death. PUMA is required for resolution of pneumococcal pneumonia in mice, as mice deficient of PUMA exhibit greater numbers of S. pneumoniae CFU within tissues and higher mortality rates than observed in Puma+/+ mice. Methods: Puma+/+ and Puma-/- mice were intranasally challenged with TIGR4 pneumococcus and sacrificed 24 h post-infection. Differences in cytokine levels from blood and whole lung tissue were detected by MILLIPLEX MAP Mouse Cytokine/Chemokine Magnetic Bead Panel. Lung transcriptomes from Puma+/+ and …

Effects Of Flex Periods On High School Student Failure Rates, Michael Ongie

Leadership Education Capstones

Schools, in their current form, do not allow for personalized amounts of time for students to learn concepts. Students do not have the opportunity to meet with teachers during the school day to get support on specific concepts they do not understand. The purpose of this study is to research the potential link between implementation of a flex period and student achievement, measured by failure rates, in high school semester courses. The study investigates how failure rates changed after the implementation of a flex period. The sample population for this study are all students who attended a specific small rural …

Subclinical Atherosclerosis Quantified Through Cumulative Shear Measurement, Margaret Lynne Papka

Master's Theses

With the high mortality rate of cardiovascular disease, it is important to study the early signs. The early detection of cardiovascular disease can lead to saved lives. Currently the most prevalent detection methods are the Framingham Risk Score and the carotid intima media thickness, both of which are insufficient. The necessary tool for early detection requires a uniform quantification system. The stimulus leading to endothelial dysfunction, the most significant predictor of a major adverse cardiovascular event (MACE)—and subsequently subclinical atherosclerosis—is reduced shear stress. Increased surface relative roughness affects the flow profile transition from laminar to turbulent resulting in reduced shear …

Quantification Of Compound And Cascading Hydroclimatic Extreme Events, Sourav Mukherjee

All Dissertations

Compound and cascading hydroclimatic extreme events have garnered much attention in recent studies. The combined effects of interconnected extremes can cause widespread damage, with a higher potential impact than individual extremes. Both anthropogenic warming and natural climate variability affect these extremes, which is why detecting past extreme events, understanding the underlying mechanisms, and assessing their future impacts can aid in mitigation efforts to reduce their overall impact. However, thus far, identifying such events is oversimplified and the propagation of their impact as cascades from the physical to human systems remains partly explored.

The overreaching goal of this thesis is to …

Development Of A Highly Sensitive Pressure Sensing System With Custom-Built Software For Continuous Physiological Measurements, Masoud Panahi

Masters Theses

In this work, a pressure sensing system was designed and fabricated by developing a highly sensitive cone-structured pressure sensor with a custom-built software for physiological monitoring applications. A novel highly sensitive cone structured porous polydimethylsiloxane (PDMS) based pressure sensor capable of detecting very low-pressure ranges was developed for respiration monitoring. The pressure sensor was fabricated using a master mold, a hybrid-structured dielectric layer, and fabric-based electrodes. The master mold with inverted cone structures was created using a rapid and precise three-dimensional (3D) printing technique. The dielectric layer, with pores and cone structures, was prepared by annealing a mixture of PDMS, …

Background Flow Influence On Impulsive Jet-Driven Micro Droplet Dispersal, Vaishak Thiruvenkitam

Doctoral Dissertations and Master's Theses

Accurately predicting the airborne spread of infectious diseases is crucial in controlling the COVID­19 pandemic today. Studies have shown the spread of expiratory droplets depends on ambient thermodynamic conditions and flow properties of the jet emitted by the activity. However, the droplet spread in conditions of background flow is not yet comprehensively understood. This study uses the Eulerian­-Lagrangian model with k−ω turbulence modelling to simulate spread of particles and study the factors affecting it in a closed environment with a background flow. Respiratory activities are modeled as a non-­isothermal jet of air with droplets suspended in them in a predetermined …

Design, Development, And Testing Of Research Payloads On Various Suborbital Flight-Test Platforms, Vijay Vishal Duraisamy

Doctoral Dissertations and Master's Theses

With recent advances in the commercial space industry, suborbital payload launches have become more common and accessible to researchers actively seeking solutions for problems involving prolonged space travel and future missions to Moon and Mars. Suborbital payload missions compared to orbital launches are less expensive and offer faster turnaround times; however, the novelty of this domain provides unique challenges. This multidisciplinary research effort aims to tackle some of these challenges by detailing the design, development, and testing techniques followed in the successful launch and recovery of payload experiments in currently active and upcoming suborbital launch vehicles. The research methodology involves …

"When The Voices Of Children Are Heard": Evaluating The Biological Effects Of Socioeconomic Status On Children In Postmedieval London., Ashley R. Ezzo

Electronic Theses and Dissertations

As the most vulnerable members of society, children (and their treatment) may reveal important sociocultural and socioeconomic praxes throughout human history. During the Postmedieval period, children, especially low socioeconomic status (SES) individuals, participated in the workforce. By examining the demographic distributions and paleopathological profiles of juvenile skeletal remains from high SES (St. Bride’s Crypt and Chelsea Old Church) and low SES (St. Bride’s Lower and Crossbones) Postmedieval (1700-1850 CE) London cemeteries, we can better understand how SES impacted the biological health and mortality of children. While results indicated higher mortality risk associated with low SES circumstances, most differences in pathological …

Anatomy And Physiology Preparatory Course Textbook (2nd Edition), Carlos Liachovitzky

Open Educational Resources

The goal of this preparatory textbook is to give students a chance to become familiar with some terms and some basic concepts they will find later on in the Anatomy and Physiology course, especially during the first few weeks of the course.

Organization and functioning of the human organism are generally presented starting from the simplest building blocks, and then moving into levels of increasing complexity. This textbook follows the same presentation. It begins introducing the concept of homeostasis, then covers the chemical level, and later on a basic introduction to cellular level, organ level, and organ system level. This …

Identification Of Leishmania Spp. And T. Cruzi Parasites In Bats Captured In El Paso, Texas Region: Bats As A New Reserviour, Edith Sandoval

Open Access Theses & Dissertations

Background: Recognized by the World Health Organization (WHO) as two of the world’s thirteen most neglected tropical diseases (NTD), both T. cruzi and Leishmania spp. parasites are accountable for Chagas disease and Leishmaniasis, respectively and they continue to rank among the most important public health problems in South America. Together, Leishmaniasis and Chagas affect 300 million people. Wildlife reservoirs play an important role in the maintenance and transmission of parasites in sylvatic transmission cycles. In the last decade studies have been conducted in this region of El Paso, Texas, and other parts of the world to detect both parasites in …

Tumor-On-Chip Modeling Of Organ-Specific Cancer And Metastasis, Nuala Del Piccolo, Venktesh S Shirure, Ye Bi, S Peter Goedegebuure, Sepideh Gholami, Christopher C W Hughes, Ryan C Fields, Steven C George

2020-Current year OA Pubs

Every year, cancer claims millions of lives around the globe. Unfortunately, model systems that accurately mimic human oncology - a requirement for the development of more effective therapies for these patients - remain elusive. Tumor development is an organ-specific process that involves modification of existing tissue features, recruitment of other cell types, and eventual metastasis to distant organs. Recently, tissue engineered microfluidic devices have emerged as a powerful in vitro tool to model human physiology and pathology with organ-specificity. These organ-on-chip platforms consist of cells cultured in 3D hydrogels and offer precise control over geometry, biological components, and physiochemical properties. …

Future Space Missions And Human Enhancement: Medical And Ethical Challenges, Konrad Szocik, Mark Shelhamer, Martin Braddock, Francis A. Cucinotta, Chris Impey, Pete Worden, Ted Peters, Milan M. Ćirković, Kelly C. Smith, Koji Tachibana, Michael J. Reiss, Ziba Norman, Arvin M. Gouw, Gonzalo Munévar

Health Physics and Diagnostic Sciences Faculty Research

Future human space missions to Mars and beyond may be realized for different research, economic, political or survival reasons. Since space remains a hazardous environment for humans, space exploration and exploitation requires the development and deployment of effective countermeasures. In this paper, we discuss prospects for human enhancement by gene editing, synthetic biology, or implants, for the purposes of future space missions. We argue that there are good reasons to consider such options, and that ethical arguments can be made in favor of human enhancement to enable long-term space exploration.

Examination Of Flow Patterns During Droplet Formation And In Venous Valve Mimic Using Μ-Piv, Vignesha Vijayananda

Master's Theses

In this work, particle image velocimetry (PIV) was used for fluid flow visualization in both continuous and segmented-flow microfluidics. Droplet microfluidics is known for its precise and consistent volume dispersion between microliters and picoliters in volume for application ranging from molecular synthesis, drug discover, and diagnostics. But the influence of junction geometry on the process of drop formation has not been investigated. In this study, µ-PIV was used to study the internal flow during the drop formation process in flow-focus microfluidic device with and without constriction. It was found that in case of flow-focus with constriction, the shear force on …

Examining The Effects Of Kratom Alkaloids On Mesolimbic Dopamine Release, James Paul Manus

Electronic Theses and Dissertations Archive

Kratom, derived from the plant Mitragyna speciosa, is reported to produce stimulant-like effects at low doses and opiate-like effects at high doses. Mitragynine (MG) and 7-hydroxymitragynine (7-HMG) are the major psychoactive constituents of kratom, but the neural mechanisms of these alkaloids are not clear. Given that the effects of kratom are often compared to those of drugs with a high abuse liability, the current study aimed to determine the effect of MG and 7-HMG on reward-related neurotransmission. In vivo fixed potential amperometry was used to quantify stimulation-evoked phasic dopamine release in the nucleus accumbens (NAc) of anesthetized male and female …

Ruminant Milk-Derived Extracellular Vesicles: A Nutritional And Therapeutic Opportunity?, Siew Ling Ong, Cherie Blenkiron, Stephen Haines, Alejandra Acevedo-Fani, Juliana A.S. Leite, Janos Zempleni, Rachel C. Anderson, Mark J. Mccann

Department of Nutrition and Health Sciences: Faculty Publications

Milk has been shown to contain a specific fraction of extracellular particles that are reported to resist digestion and are purposefully packaged with lipids, proteins, and nucleic acids to exert specific biological effects. These findings suggest that these particles may have a role in the quality of infant nutrition, particularly in the early phase of life when many of the foundations of an infant’s potential for health and overall wellness are established. However, much of the current research focuses on human or cow milk only, and there is a knowledge gap in how milk from other species, which may be …

Optimization Of Mechanical Properties At The Infarct Border Zone, Kaushik Sunder

LSU Master's Theses

A myocardial infarction (MI) occurs every 39 seconds in the United States. As of 2016, an estimated 550,000 new and 200,000 recurrent episodes of myocardial infarction (MI) occur each year in the United States was. Infarction is often distinguished by a considerable decrease in wall thickness and an increase in heart size or left ventricular volume. This behavior happens when there is a prolonged lack of blood flow, usually due to damage or clotting, which causes cardiomyocytes to die (CMs). The development of non-contractile infarct scarring is the primary pathophysiology of MI. While the infarct scar prevents myocardial rupture during …

Initial Investigations Into Tools For Phytoremediation Of Lead From Urban And Suburban Landscapes, Michael Taylor Bryant

LSU Master's Theses

Lead is a pervasive and ongoing problem in the landscape, especially in urban and suburban areas where historic use of tetraethyl lead gasoline and leaded paint has increased background amounts to dangerous levels. One method to solve this problem is the use of hyperaccumulator plant species, capable of concentrating over 1000 ppm Pb into their tissues. This is a slow process, even with hyperaccumulators, so the use of amendments to improve Pb solubility has gained popularity, though with mixed results. This study examined Trachelospermum asiaticum, Ardisia japonica, and Ophiopogon japonicus, three common landscape groundcovers used in Louisiana, …

Editorial: Invertebrate Neuroscience: Contributions From Model And Non-Model Species, Maria P. Fernandez, Clare C. Rittschof, Jimena A. Sierralta

Entomology Faculty Publications

No abstract provided.

Effects Of Cod Liver Oil On Brain Antioxidant Level And Neuronal Count In Wistar Rat Model Of Comorbid Depression, Dimple Shet

Faculty work

No abstract provided.

A Deep Learning Algorithm To Translate And Classify Cardiac Electrophysiology, Parya Aghasafari, Pei-Chi Yang, Divya C. Kernik, Kazuho Sakamoto, Yasunari Kanda, Junko Kurokawa, Igor Vorobyov, Colleen E. Clancy

Open Access Publications

The development of induced pluripotent stem cell-derived cardiomyocytes (iPSC-CMs) has been a critical in vitro advance in the study of patient-specific physiology, pathophysiology, and pharmacology. We designed a new deep learning multitask network approach intended to address the low throughput, high variability, and immature phenotype of the iPSC-CM platform. The rationale for combining translation and classification tasks is because the most likely application of the deep learning technology we describe here is to translate iPSC-CMs following application of a perturbation. The deep learning network was trained using simulated action potential (AP) data and applied to classify cells into the drug-free …

Aacp Basic Resources For Pharmacy Education, Jason Guy, Ivan Portillo, Robert D. Beckett, Leslie Ann Bowman, Daisy De La Rosa, Vern Duba, Juanita Draime, Kayce Gill, Neyda Gilman, Rebecca Hoover, Alison Kosnieczny, Scott Perkins

Library Association/Organization Work

"The AACP Basic Resources for Pharmacy Education is produced as a guide for those developing or maintaining the library collections that serve colleges and schools of pharmacy. The goal of the Basic Resources list is to make recommendations of books and other works to be included in pharmacy libraries, but not all titles are required to be purchased. Each pharmacy college has its own mission and its own program(s), and so each college’s library collection must reflect that mission and support the college’s program(s). Excellent library collections are built by knowledgeable librarians and drug information specialists using their professional judgment …