Digital Commons Network^™

The Fight Over Frankenmeat: The Fda As The Proper Agency To Regulate Cell-Based “Clean Meat”, Zoe A. Bernstein

Brooklyn Law Review

In recent years, concern over the environmental, animal welfare, and human costs of animal agriculture has spurred an increased demand for nonanimal sourced protein. This has led to significant innovation in food technology. As part of this trend, food scientists have developed a process for in-vitro cultivation of meat cells to produce protein that is biologically and nutritionally identical to meat from traditionally raised and slaughtered animal sources, but that involves neither animal agriculture nor animal slaughter. This lab-grown “clean meat” represents a new era in food technology and is already having an effect on the existing meat industry. In …

Autoradiographic Localization Of [3h]-Nisoxetine Binding Sites In The Cns Of Male And Female Japanese Quail, Shannon E. Eaton, James R. Pauly, Deann M. Hopkins, Chana K. Akins

Pharmaceutical Sciences Faculty Publications

Background

In the central nervous system of mammals, transporters localized on the presynaptic nerve terminals regulate the reuptake of neurotransmitters. These transporters are selective for a specific neurotransmitter such as dopamine (DA) and norepinephrine (NE). Specifically in the synapse, the dopamine transporter (DAT) reuptakes DA and the norepinephrine transporter (NET) reuptakes NE. However previous research has found that avian species do not have a gene for DAT, and therefore, birds may be using the NET to clear both NE and DA from the synapse. The current study aimed to extend this finding by localizing NET expression in male and female …

Neuron-Derived Extracellular Vesicles Modulate Microglia Activation And Function, Hui Peng, Brock T. Harvey, Christopher I. Richards, Kimberly Nixon

Pharmaceutical Sciences Faculty Publications

Microglia act as the immune cells of the central nervous system (CNS). They play an important role in maintaining brain homeostasis but also in mediating neuroimmune responses to insult. The interactions between neurons and microglia represent a key process for neuroimmune regulation and subsequent effects on CNS integrity. However, the molecular mechanisms of neuron-glia communication in regulating microglia function are not fully understood. One recently described means of this intercellular communication is via nano-sized extracellular vesicles (EVs) that transfer a large diversity of molecules between neurons and microglia, such as proteins, lipids, and nucleic acids. To determine the effects of …

Identification Of The Adult Hematopoietic Liver As The Primary Reservoir For The Recruitment Of Pro-Regenerative Macrophages Required For Salamander Limb Regeneration., Ryan J Debuque, Andrew J Hart, Gabriela H Johnson, Nadia Rosenthal, James W Godwin

Faculty Research 2021

The lack of scar-free healing and regeneration in many adult human tissues imposes severe limitations on the recovery of function after injury. In stark contrast, salamanders can functionally repair a range of clinically relevant tissues throughout adult life. The impressive ability to regenerate whole limbs after amputation, or regenerate following cardiac injury, is critically dependent on the recruitment of (myeloid) macrophage white blood cells to the site of injury. Amputation in the absence of macrophages results in regeneration failure and scar tissue induction. Identifying the exact hematopoietic source or reservoir of myeloid cells supporting regeneration is a necessary step in …

Recognition And Screening For Chagas Disease In The Usa, Rachel Marcus, Andres F. Henao-Martinez, Melissa Nolan Ph.D., Mph, Elizabeth Livingston, Stephen A. Klotz, Robert H. Gilman, Monica Miranda-Schaeubinger, Sheba Meymandi

Faculty Publications

Chagas disease (CD), caused by the protozoan Trypanosoma cruzi, is a public health concern, mainly among countries in South and Central America. However, despite the large number of immigrants from endemic countries living in the USA, awareness of CD is poor in the medical community, and therefore it is significantly underdiagnosed. To avoid the catastrophic cardiac complications of CD and to prevent maternal–fetal transmission, widespread educational programs highlighting the need for diagnosis are urgently needed.

Dietary Saturated Fats And Health: Are The Us Guidelines Evidence-Based?, Arne Astrup, Nina Teicholz, Faidon Magkos, Dennis M Bier, J Thomas Brenna, Janet C King, Andrew Mente, José M Ordovas, Jeff S Volek, Salim Yusuf, Ronald M Krauss

Children’s Nutrition Research Center Staff Publications

The last decade has seen nearly 20 papers reviewing the totality of the data on saturated fats and cardiovascular outcomes, which, altogether, have demonstrated a lack of rigorous evidence to support continued recommendations either to limit the consumption of saturated fatty acids or to replace them with polyunsaturated fatty acids. These papers were unfortunately not considered by the process leading to the most recent U.S. Dietary Guidelines for Americans, the country's national nutrition policy, which recently reconfirmed its recommendation to limit saturated fats to 10% or less of total energy intake, based on insufficient and inconsistent evidence. Continuation of a …

The Effects Of Nursing Program Simulation Experience Level And Type Of Advanced Organizer On Clinical Judgment Performance, Satisfaction, Self-Confidence, And Perceived Cognitive Load Of Pre-Licensure Nursing Students In Simulation Based Learning, Tina D. Barbour-Taylor

Dissertations

Nursing education programs face many challenges with training students to become professional nurses who will practice safely in an evolving and complex healthcare system. Nurse educators are obliged to prepare students to meet these challenges, with clinical experience being a key aspect of training. However, limited availability of clinical sites restricts adequate training. Simulation based learning (SBL) activities that mimic real world environments are now transforming the clinical experience, however, research is limited and additional research is needed.

Super-Enhancer-Based Identification Of A Batf3/Il-2r-Module Reveals Vulnerabilities In Anaplastic Large Cell Lymphoma, Huan-Chang Liang, Mariantonia Costanza, Nicole Prutsch, Mark W. Zimmerman, Elisabeth Gurnhofer, Ivonne A. Montes-Mojarro, Brian J. Abraham, Nina Prokoph, Stefan Stoiber, Shahid Pervez

Department of Pathology and Laboratory Medicine

Anaplastic large cell lymphoma (ALCL), an aggressive CD30-positive T-cell lymphoma, comprises systemic anaplastic lymphoma kinase (ALK)-positive, and ALK-negative, primary cutaneous and breast implant-associated ALCL. Prognosis of some ALCL subgroups is still unsatisfactory, and already in second line effective treatment options are lacking. To identify genes defining ALCL cell state and dependencies, we here characterize super-enhancer regions by genome-wide H3K27ac ChIP-seq. In addition to known ALCL key regulators, the AP-1-member BATF3 and IL-2 receptor (IL2R)-components are among the top hits. Specific and high-level IL2R expression in ALCL correlates with BATF3 expression. Confirming a regulatory link, IL-2R-expression decreases following BATF3 knockout, and …

Using Praise To Increase Visual Attending In An Asynchronous Online Learning Environment: An Eye Tracking Study, Andrew J. Rozsa Iii

Master's Theses

With the emergence of online courses in the mid-1990s, the number of students enrolled in online courses has been growing at an exponential rate (Schwirzke, Vashaw, & Watson, 2018). This trend brings with it new problems, such as familiarity with evidence-supported behavioral techniques that will maintain student engagement and improve likelihood of academic success in online learning environments. The purpose of the present study was to examine how the use of praise may affect visual engagement with video lectures with the assistance of commercially available eye tracking technology. A secondary objective of the study was to identify how praise affects …

A Molecular Survey Of Bacterial Species In The Guts Of Black Soldier Fly Larvae (Hermetia Illucens) Reared On Two Urban Organic Waste Streams In Kenya, Marwa Shumo, Fathiya M. Khamis, Fidelis Levi Ombura, Chrysantus M. Tanga, Komi K.M. Fiaboe, Sevgan Subramanian, Sunday Ekesi, Oliver K. Schlüter, Arnold Van Huis, Christian Borgemeister

All Peer-Reviewed Publications

Globally, the expansion of livestock and fisheries production is severely constrained due to the increasing costs and ecological footprint of feed constituents. The utilization of black soldier fly (BSF) as an alternative protein ingredient to fishmeal and soybean in animal feed has been widely documented. The black soldier fly larvae (BSFL) used are known to voraciously feed and grow in contaminated organic wastes. Thus, several concerns about their safety for inclusion into animal feed remain largely unaddressed. This study evaluated both culture-dependent sequence-based and 16S rDNA amplification analysis to isolate and identify bacterial species associated with BSFL fed on chicken …

A Critical Perspective On The Indian Experience, K. P. Madhu

Online Journal of Space Communication

Growth in the use of satellites for education has been phenomenal in India. Beginning in the 1970s with the SITE project (Satellite Instructional Television Experiment) using a borrowed US satellite, then in the 1980s having its own indigenous INSAT satellite for educational TV, to having a full fledged educational channel on national TV in the 1990s, to the potential of 72 educational channels on EDUSAT at the beginning of the 21st century, shows long-term interest and commitment to this sector.

Futurama: An Immersive Experience Of America's Automotive Future, James Miller

Journal of Motorsport Culture & History

General Motors’ Futurama exhibit at the 1939-40 New York World’s Fair offered a wildly popular immersive experience of American automobility twenty years in the future. The Fair proclaimed the “Dawn of a New Day” in “The World of Tomorrow” through comprehensive innovative architecture and design, which promoted the primary role of new technology, especially in the field of transportation. Futurama harnessed techniques of theatre and multi-media in unprecedented ways. Its narrow aim was to foster the construction of new highway systems hospitable to the growing population of modern cars. More broadly, Futurama sought to inculcate a new way of thinking …

A Misconception About Hysteria: The Portrayal Of Women In Alfred Hitchcock's The Birds, Natalie Doublesin

Quest

Interpretive Argument Essay

Research in progress for ENGL 1302: Composition II

Faculty Mentor: Michael Schueth, Ph.D.

I am pleased to introduce Natalie Doublesin’s “A Misconception About Hysteria: The Portrayal of Women in Alfred Hitchcock’s The Birds,” an essay she wrote for my English 1302 course in the spring 2020 semester. The research assignment was drawn from the textbook Writing Analytically, which asks students to practice writing a tightly argued interpretation of a film. I chose Alfred Hitchcock’s 1963 film, The Birds, because it is wonderfully apt for interpretive work as the events in the film are not …

Two-Dimensional Monitoring Of Spatial Distribution Of Herbage Mass Under Grazing, S. Ogura, Masahiko Hirata

IGC Proceedings (1977-2023)

Spatial distribution of herbage mass (HM) was monitored two-dimensionally during the grazing season (from May to November) in a bahiagrass pasture rotationally grazed by cattle. An electronic capacitance probe was used to estimate HM in 1464 permanent positions (50 × 50 cm) whose centres formed 1.5 × 1.5 m grids in the central 90 × 34.5 m area of the pasture. The highest mean HM was in September and October, while coefficient of variation in November. The two-dimensional charting of HM showed almost stable spatial patterns throughout the grazing season, with some patches of high HM appearing and disappearing. The …

The Effect Of Herbage Mass Of A Pasture On The Spatially Heterogeneous Grazing By Cattle, S. Ogura, Masahiko Hirata

IGC Proceedings (1977-2023)

To obtain information on spatially heterogeneous grazing by large herbivores at different time scales, spatial distribution of herbage mass and rate of defoliation was investigated on a bahiagrass (Paspalum notatum Flügge) pasture grazed by cattle during 4 to 5-day grazing periods in May, August and October. Pre-grazing herbage mass (M_pre) showed decreasing mean values and increasing spatial heterogeneity with the progress of grazing in all grazing periods, while the rate of defoliation showed inconsistent changes with season. Spatial distribution of the rate of defoliation was always more heterogeneous than that of M_pre. The correlation coefficient …

Relationships Between Spatial Distributions Of Herbage Mass And Utilization In A Pasture Progressively Grazed By Cattle, Masahiko Hirata, S. Ogura

IGC Proceedings (1977-2023)

To obtain some information about the mechanisms behind the spatially heterogeneous grazing of a pasture by large herbivores, the relationships between the spatial distributions of herbage mass, herbage consumption and ingestive behavior were investigated on a bahiagrass (Paspalum notatum Flügge) pasture during a 6-day grazing period with cattle. The animals almost always consumed more herbage from locations with higher pre-grazing herbage masses, which was attributed to greater bite weights. It was suggested that the high spatial heterogeneity in the rate of defoliation was caused mainly by the spatial heterogeneity in both the bite weight and the number of visits …

Based On Atmospheric Physics And Ecological Principle To Assess The Accuracies Of Field Co2 /H2o Measurements From Infrared Gas Analyzers In Closed-Path Eddy-Covariance Systems, Xinhua Zhou, Tian Gao, Yunchao Pang, Hayden Mahan, Xiufen Li, Ning Zheng, Andrew E. Suyker, Tala Awada, Jiaojun Zhu

School of Natural Resources: Faculty Publications

Field CO₂ /H₂O measurements from infrared gas analyzers in closed-path eddy-covariance systems have wide applications in earth sciences. Knowledge about exactness of these measurements is required to assess measurement applicability. Although the analyzers are specified with uncertainty components (zero drift, gain drift, cross-sensitivities, and precision), exactness for individual measurements is unavailable due to an absence of methodology to comprehend the components as an overall uncertainty. Adopting an advanced definition of accuracy as a range of all measurement uncertainty sources, the specified components are composited into a model formulated for studying analyzers’ CO₂ /H₂O accuracy …

Mla Works Cited Entries For Various Sources, Angela Gulick

MLA (Modern Language Association) Documentation

This handout provides guidelines for constructing works cited entries for papers requiring Modern Language Association (MLA) style.

Whole-Genome Sequencing To Investigate A Possible Genetic Basis Of Perosomus Elumbis In A Calf Resulting From A Consanguineous Mating, Alexa M. Barber, Alyssa Helms, Riley Thompson, Brian K. Whitlock, David J. Steffen, Jessica Lynn Petersen

Department of Animal Science: Faculty Publications

Perosomus elumbis (PE) is a lethal, congenital defect marked by aplasia of the lumbar and sacral spine and spinal cord. Contracture of the hind limbs is also commonly observed in affected individuals. PE has been reported in many domestic species, with numerous case reports in Holstein cattle in the past two decades (Jones, 1999; Karakaya et al., 2013; Agerholm et al., 2014). The etiology of PE remains unknown. In one instance, a stillborn Holstein calf with PE was found to be infected with Bovine Viral Diarrhea Virus (BVDV) (Karakaya et al., 2013), and thus, it is possible that PE may …

Risk Of Hemolytic Uremic Syndrome Related To Treatment Of Escherichia Coli O157 Infection With Different Antimicrobial Classes, Rajal K. Mody, Robert M. Hoekstra, Magdalena Kendall Scott, John Dunn, Kirk Smith, Melissa Tobin-D'Angelo, Beletshachew Shiferaw, Katie Wymore, Paula Clogher, Amanda Palmer, Nicole Comstock, Kari Burzlaff, Sarah Lathrop, Sharon Hurd, Patricia M. Griffin

Pathology Research and Scholarship

Treatment of Shiga toxin-producing Escherichia coli O157 (O157) diarrhea with antimicrobials might alter the risk of hemolytic uremic syndrome (HUS). However, full characterization of which antimicrobials might affect risk is lacking, particularly among adults. To inform clinical management, we conducted a case-control study of residents of the FoodNet surveillance areas with O157 diarrhea during a 4-year period to assess antimicrobial class-specific associations with HUS among persons with O157 diarrhea. We collected data from medical records and patient interviews. We measured associations between treatment with agents in specific antimicrobial classes during the first week of diarrhea and development of HUS, adjusting …

Multi-Agent Scavenging Patterns In Hawai‘I: A Forensic Archaeological And Skeletal Case Study, Jennifer F. Byrnes, William Belcher

Department of Anthropology: Faculty Publications

Knowledge of the behavior of local fauna can aid forensic investigators in developing awareness of site formation processes. In Hawai‘i, little has been published on the effects of feral domestic pig (Sus scrofa) and feral domestic dog (Canis familiaris) scavenging and bone dispersal on field recovery and laboratory observations. In this Pacific tropical setting, the most consequential terrestrial taphonomic agents are pigs and dogs, both in terms of hard tissue modification and dispersal of remains across the landscape. In 2017, an archaeologist discovered the remains of an unidentified decedent on the island of Kauaʻi, State of Hawai‘i during a cultural …

Diabetic Aldehyde Dehydrogenase 2 Mutant (Aldh2*2) Mice Are More Susceptible To Cardiac Ischemic-Reperfusion Injury Due To 4-Hydroxy-2-Nonenal Induced Coronary Endothelial Cell Damage, Guodong Pan, Bipradas Roy, Suresh S. Palaniyandi

Hypertension and Vascular Research Articles

Background: Aldehyde dehydrogenase-2 (ALDH2), a mitochondrial enzyme, detoxifies reactive aldehydes such as 4-hydroxy-2-nonenal (4HNE). A highly prevalent E487K mutation in ALDH2 (ALDH2*2) in East Asian people with intrinsic low ALDH2 activity is implicated in diabetic complications. 4HNE-induced cardiomyocyte dysfunction was studied in diabetic cardiac damage; however, coronary endothelial cell (CEC) injury in myocardial ischemia-reperfusion injury (IRI) in diabetic mice has not been studied. Therefore, we hypothesize that the lack of ALDH2 activity exacerbates 4HNE-induced CEC dysfunction which leads to cardiac damage in ALDH2*2 mutant diabetic mice subjected to myocardial IRI.

Methods and Results: Three weeks after diabetes mellitus (DM) induction, …

Hydrophobically Modified Polypeptoids And Uses Thereof, Donghui Zhang, Vijay T. John, Yueheng Zhang, Sunting Xuan

College of Science Patents

A variety of hydrophobically modified polypeptoids are provided. The hydrophobically modified polypeptoids can include a polyamide backbone having a random copolymer of two or more different types of repeat units, where one or more of the repeat units have nitrogen atom having a hydrophobic substituent attached thereto. Methods of making the hydrophobically modified polypeptoids are also provided, as well as uses of the hydrophobically modified polypeptoids, for example in liposomal drug delivery.

Targeted Delivery Of Molecules Using Impedance-Based Monitoring At Elevated Temperatures, Richard Heller, Loree C. Heller, Mark Jeffery Jaroszeski

USF Patents

A method and system for delivering a molecule to a specific area of a tissue by controlling temperature and impedance is presented. The method is generally comprised of applying heat to a biological structure, such as cells or tissues, to heat the biological structure to a preset temperature after which at least one electroporation pulse is administered to the biological structure. Impedance is measured as a feedback control mechanism after each pulse and pulse parameters are adjusted accordingly until desired impedance is reached. The system generally comprises an electroporation system capable of generating at least one pulse, measuring impedance and …

Iron(Iii) Chelated Paramagnetic Polymeric Nanoparticle Formulation As A Next-Generation: T 1-Weighted Mri Contrast Agent, Ramesh Marasini, Sagar Rayamajhi, Anthony Moreno-Sanchez, Santosh Aryal

Pharmacy Faculty Publications and Presentations

Magnetic resonance imaging (MRI) is a routinely used imaging technique in medical diagnostics. To enhance the quality of MR images, contrast agents (CAs) are used, which account for nearly 40% of MRI exams in the clinic globally. The most used CAs are gadolinium-based CAs (GBCAs) but the use of GBCAs has been linked with metal-deposition in vital organs. Gadolinium deposition has been shown to be correlated with nephrogenic systemic fibrosis, a fibrosis of the skin and internal organs. Therefore, there is an unmet need for a new CA alternative to GBCAs for T 1-weighted Ce-MRI. Herein, we designed paramagnetic ferric …

Mitochondrial Proteins In Mediating Colon Cancer And Potential Theraputics, Kunlun Yin

Chemistry and Chemical Biology ETDs

Cancer is one of the biggest threatens to human health. Many people died because of various types of cancer. Colon cancer is one of the leading causes of new incidence and mortality in the whole world. It is important to understand the mechanism of tumorigenesis and development. Based on the mechanisms, we may develop new therapeutics to improve survival rate. In Chapter 2, we discussed the PINK1 function in suppression colon cancer. We found that PINK1 deficiency can promote colon cancer, and PINK1 overexpression suppressed tumor growth in vivo and in vitro. PINK1 can activate its substrate P53, which activates …

The Stability Of The Critical Points Of The Generalized Gause Type Predator-Prey Fishery Models With Proportional Harvesting And Time Delay.Pdf, Wan Natasha Wan Hussin, Rohana Embong, Che Noorlia Noor

Malaysian Journal of Computing (MJoC)

In the marine ecosystem, the time delay or lag may occur in the predator response function, which measures the rate of capture of prey by a predator. This is because, when the growth of the prey population is null at the time delay period, the predator’s growth is affected by its population and prey population densities only after the time delay period. Therefore, the generalized Gause type predator-prey fishery models with a selective proportional harvesting rate of fish and time lag in the Holling type II predator response function are proposed to simulate and solve the population dynamical problem. From …

Antibiotic Resistance In The Patient With Cancer: Escalating Challenges And Paths Forward, Amila K. Nanayakkara, Helen W. Boucher, Vance G. Fowler Jr., Amanda Jezek, Kevin Outterson, David E. Greenberg

Faculty Scholarship

Infection is the second leading cause of death in patients with cancer. Loss of efficacy in antibiotics due to antibiotic resistance in bacteria is an urgent threat against the continuing success of cancer therapy. In this review, the authors focus on recent updates on the impact of antibiotic resistance in the cancer setting, particularly on the ESKAPE pathogens (Enterococcus faecium, Staphylococcus aureus, Klebsiella pneumoniae, Acinetobacter baumannii, Pseudomonas aeruginosa, and Enterobacter spp.). This review highlights the health and financial impact of antibiotic resistance in patients with cancer. Furthermore, the authors recommend measures to control …

Synthesis And Antiviral Activity Of Fatty Acyl Conjugates Of Remdesivir Against Severe Acute Respiratory Syndrome Coronavirus 2 And Ebola Virus, Naglaa Salem El-Sayed, Alexander S. Jureka, Megan R. Edwards, Sandeep Lohan, Caroline G. Williams, Patrick T. Keiser, Robert A. Davey, Jennifer Totonchy, Rakesh Kumar Tiwari, Christopher F. Basler, Keykavous Parang

Pharmacy Faculty Articles and Research

We report here the synthesis, purification, and characterization of mono- and di-fatty acyl conjugates of remdesivir (RDV) and their in vitro antiviral activity against SAR-CoV-2, an Ebola virus transcription- and replication-competent virus-like particle (trVLP) system, and infectious Ebola virus. The most potent monofatty acyl conjugate was 4b, containing a 4-oxatetradecanolyl at the 3′ position. Monofatty acyl conjugates, 3′-O-tetradecanoyl (4a) (IC_50(VeroE6) = 2.3 μM; IC_50(Calu3) = 0.24 μM), 3′-O-4-oxatetradodecanoyl (4b) (IC5_0(VeroE6) = 2.0 μM; IC_50(Calu3) = 0.18 μM), and 3′-O-(12-ethylthiododecanoyl) (4e) (IC_50(VeroE6) = 2.4 μM; IC_50(Calu3) = 0.25 μM) …

Opening Peer Review In Lis: Identities, Dualities, And Multiplicities, Emily Ford

Library Faculty and Staff Publications and Presentations

What does it mean to peer review in library and information science? What does it mean to be reviewed? How do our professional identities intersect with this vital research and publishing role? And what does it mean when peer review is opened to reveal these identities? In celebration of Peer Review Week 2021, this free webcast with Emily Ford will share insights into peer review in LIS as discussed in Stories of Open: Opening Peer Review through Narrative Inquiry, a newly published book from ACRL.