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The Gut Microbiome Of Hapalemur Griseus Griseus, Propithecus Diadema, And Propithecus Coquereli: A Comparative Study Between Wild And Captive Lemur Species, Brenna Ritchie

Graduate Research Theses & Dissertations

Madagascar is home to thousands of endemic species, making it a biodiversity hotspot that is rapidly losing its fauna due to deforestation and human expansion. Efforts to restore and preserve this unique island are ongoing and several researchers have placed their focus on preventing species extinctions. One research avenue that can help contribute to these efforts is to study health and wellness of animals located in areas affected by these changes. In this study, I sought to characterize microbial community composition of two native lemur species found in Madagascar and compare those profiles to similar species found in captivity in …

Immediate Response Of Black Basses To Dam Removal Is Determined By Habitat Preference, Reuben Frey

Masters Theses

Dams affect the abundance of fish species in lotic systems by altering flow regime and available physical habitat. Removal of dams may mitigate these effects and generate a change in species abundance and fish community structure. Understanding the effects of dam removal on gamefish species is essential in making management decisions. I investigated how populations of Smallmouth Bass (Micropterus dolomieu), Spotted Bass (M. punctulatus), and Largemouth Bass (M. salmoides) were affected by the presence and removal of two low-head dams in a Midwestern river system. I used data collected during fall and spring from …

The Effect Of Mortality Salience On Compromising Mate Selection Standards In Homosexual And Heterosexual Individuals, Sydney Rohmann

Masters Theses

Terror management theory (TMT), proposed by Greenberg, Pyszcynski, and Solomon (1986), suggests that humans cope with terror resulting from the knowledge of their own mortality. The need for coping mechanisms arises when individuals are reminded of their own inevitable death; that is, when they experience mortality salience (MS). Hirschberger, Florian, and Mikulincer (2002) found that when primed with death reminders, heterosexual individuals tend to compromise their ideal mate selection to form close relationships. There has also been extensive research on the differences between homosexual and heterosexual mating preferences. This study examined the effect mortality salience has on an individuals’ mate …

Development Of Fluorescence Microscopy Approaches To Study Subcellular Protein Transport And Enzymatic Activity, Anchal Singh

Masters Theses

Understanding the subcellular localization of proteins and their activity is important in understanding their normal function in eukaryotic cells. Fluorescence cellular imaging techniques can selectively and sensitively visualize subcellular biochemistry. Using this approach, two different methods were employed in this thesis. The first focused on studying protein import into peroxisome and the other on monitoring the activity of an endoplasmic reticulum (ER)-localized enzyme, human carboxylesterase 1 (CES1).

Peroxisomes are mainly known as the center for long chain fatty acid b-oxidation as well as the production and detoxification of hydrogen peroxide. Proteins which are needed in the peroxisomes are encoded in …

Xylan-Derived Carbon Nutrient Acquisition By Caulobacter Crescentus, Rhoda Ekuama Inkoom

Masters Theses

Bacterial decomposition of xylan and uptake of xylan-derived carbon compounds require a combination of enzymes, proteins, and complex molecular mechanisms. Bacteria have evolved molecular mechanisms for its utilization depending on its environment and source of xylan. Caulobacter crescentus, a Gram-negative bacterium which lives in an aquatic oligotrophic environment, exhibits asymmetric division which is an adaptive feature for its nutrient poor habitat. Although aromatic hydrocarbon and cellulose-derived mono-, di- and oligosaccharide utilization has been reported in literature, its xylan-derived carbon compound utilization is yet to be characterized. In this study, C. crescentus utilizes xylan-derived monomers arabinose, xylose and acetate as …

Developing Synthetic Strategies For Multifaceted Applications Of Stable Gold-Based Complexes, Randall Tyler Mertens

Theses and Dissertations--Chemistry

Development of stable gold-based complexes has been a rapidly advancing field due to the popularity of gold complexes, particularly for use in biomedical research and catalytic transformations. Given that auranofin, a gold(I) complex with FDA approval for the treatment of rheumatoid arthritis is used in the clinic, the development of stable gold-based molecules of clinical relevance is urgently needed. Herein are reported, synthetic strategies used for the development of new classes of gold(I) and gold(III) complexes for advancement in mitochondrial modulation for use as chemotherapeutics as well as application to gold catalysis due to the unique geometry of complexes presented …

Memory Of The Macabre, Miko Yoshida

Mighty Pen Project Anthology & Archive

A marine contemplates life, death, and veganism via the death of a family cow.

Articles, stories, and other compositions in this archive were written by participants in the Mighty Pen Project. The program, developed by author David L. Robbins, and in partnership with Virginia Commonwealth University and the Virginia War Memorial in Richmond, Virginia, offers veterans and their family members a customized twelve-week writing class, free of charge. The program encourages, supports, and assists participants in sharing their stories and experiences of military experience so both writer and audience may benefit.

Dog Eat Dog, Jonathan Pucci

Mighty Pen Project Anthology & Archive

Returning from deployment, a marine adjusts to being a full-time father.

Articles, stories, and other compositions in this archive were written by participants in the Mighty Pen Project. The program, developed by author David L. Robbins, and in partnership with Virginia Commonwealth University and the Virginia War Memorial in Richmond, Virginia, offers veterans and their family members a customized twelve-week writing class, free of charge. The program encourages, supports, and assists participants in sharing their stories and experiences of military experience so both writer and audience may benefit.

Clinical Applications And Feasibility Of Proton Ct And Proton Radiography, Christina Marie Sarosiek

Graduate Research Theses & Dissertations

Proton therapy is a form of radiation treatment for cancer that utilizes the Bragg peak to create conformal high dose regions around the tumor volume. However, the use of x-ray computed tomography (CT) and x-ray radiography for treatment planning and pre-treatment quality assurance procedures improves the achievable effectiveness of proton treatment plans (using proton CT) and the pretreatment verification (using proton radiography). Errors in the conversion from x-ray Hounsfield units (HU) to proton relative stopping powers (RSP) leads to errors in the predicted proton range. To account for the errors, 3.5% margins are included in the treatment plan. This means …

Cue Polarization And Representation In Mouse Home-Base Establishment, Ericka Ann Schaeffer

Graduate Research Theses & Dissertations

Many individuals suffer from navigational deficits and subsequent topographical disorientation, yet there is debate over how navigation to a goal is represented within the brain. Early research suggests animals represent their environment in a Cartesian coordinate system which manifests into a cognitive map. Current research suggests animals use a vector-based system which gives rise to a directional response. These studies examined responses to the environment when there was a specific end goal (i.e., hidden platform, food reward, etc.), versus an animal’s internal goal, home-base. The first study used mice to examine the influence of visual and tactile cues on the …

The Impact Of An Immersive Virtual Reality Learning Environment With Computer-Aided Design And Three-Dimensional Modeling Through Stem Middle-School Education Programming, Danielle Eve Schneider

Graduate Research Theses & Dissertations

Immersive virtual reality as a tool in the middle-school classroom is a promising avenue to aid in cognitively challenging tasks requiring access to visual processes. As these tools become ever present in society, the expectation of school districts to include them into classroom curricula has become challenging, as there is inconsistent data connecting to beneficial learning outcomes. Middle-School STEM classrooms experience an exceptional challenge in the expectation to build strong spatial reasoning skills on rudimentary geometry concepts. It is with that in mind that many 21st-century technology classrooms turn to immersive virtual reality, such as Oculus Rift, as a tool …

Diversity And Taxonomy Of Malagasy Pygmy Hippopotamuses, Allison Shackelton

Graduate Research Theses & Dissertations

Madagascar has many species of extinct megafauna, including up to three species of extinct hippopotamus. Subfossil hippo specimens have been found at sites across Madagascar, yet the number of species and taxonomic relationships of the extinct Malagasy pygmy hippopotamus are poorly understood. This thesis will analyze cranial and postcranial bones to improve our understanding of the diversity and taxonomy of Malagasy subfossil hippos, focusing on adults from Ampasambazimba and Tsaramody and juveniles from Ampasambazimba and Anjohibe. Measurements of the tibia and femur of adult specimens, which are used as diagnostic features in other studies, may not be accurate diagnostic features, …

Midwestern U.S. Diurnal Temperature Range: Spatial And Temporal Trends From 1900-2018, Kelly Ann Swaney

Graduate Research Theses & Dissertations

In the Corn Belt region of the United States, the twentieth century saw many land use changes as the land had been converted from the natural landscape to cropland. As the twentieth century progressed, numerous advancements occurred to increase the crop acreage, crop density, and amount of irrigated land. All of these changes contributed to higher rates of evapotranspiration, which put more moisture into the low levels of the atmosphere. This additional moisture played a role in changing the radiative fluxes and, as a result, the surface temperature. The Diurnal Temperature Range (DTR) is examined across the 1900 to 2018 …

The Influence Of Defendants' Nonverbal Behaviors On Juror Liking, Sympathy, And Sentencing, Joseph Thomas

Graduate Research Theses & Dissertations

Jurors are highly susceptible to influence, especially in the form of emotional manipulation. Totest this, the present study applies Burgoon’s (1993) expectancy violation theory to affective defendant behaviors (i.e., remorse, immediacy). In an attempt to manipulate the perceived rewardingness of the defendant, these behaviors are coupled with a description of either a major or minor crime. The results indicate that remorse behaviors evoke sympathy, thereby leading to a more lenient sentencing recommendation by mock jurors. Though the likeability of the defendant also impacted juror sentencing recommendations, immediacy behaviors failed to produce such an effect. Implications of these results are discussed.

Object Detection And Tracking With Occlusion Handling In Autonomous Vehicles, Bhuvan Tiruttani Reddy

Graduate Research Theses & Dissertations

Object detection and tracking are important modules that need to be mastered for safe autonomous driving. Various detection and tracking algorithms have been developed in the past decade to detect and track objects accurately and efficiently in a variety of scenarios. However, most of them perform poorly during occlusion and are unable to reassociate the object IDs after the event of occlusion. In this thesis, a single unified algorithm is developed to detect, track, and display the trajectories of multiple objects in real-time, in the perceptive surroundings of an autonomous car. An Enhanced DeepSORT (EDS) tracker is proposed to better …

Mass Spectrometry Method Development For The Discovery And Characterization Of Secondary Metabolites, Zachary Kelley

Theses and Dissertations--Chemistry

Secondary metabolites are organic compounds produced by an organism for reasons other than growth and development. In plants, secondary metabolites generally act as defense agents produced to deter predators and inhibit other competitive species. For humans, these compounds can often have a beneficial effect and are pursued and utilized as natural pharmaceuticals. The development of sensitive, high-throughput analytical screening methods for plant derived metabolites is crucial for natural pharmaceutical product discovery and plant metabolomic profiling. Here, metabolomic profiling methods were developed using a microfluidic capillary zone electrophoresis device and evaluated against traditional separation approaches. An alkaloid screening assay was constructed …

Flavin Modification And Redox Tuning In The Bifurcating Electron Transfer Flavoprotein From Rhodopseudomonas Palustris: Two Arginines With Different Roles, Nishya Mohamed-Raseek

Theses and Dissertations--Chemistry

Electron bifurcation is considered as a third fundamental mode of energy conservation mechanism in addition to two well-known mechanisms, substrate level phosphorylation and Oxidative phosphorylation, in electron bifurcation endergonic and exergonic redox reactions are coupled. The newly discovered flavin based electron bifurcation in electron transfer flavoproteins (ETFs) helps to reduce low potential ferredoxin, which provides electrons to drive biologically demanding reactions such as atmospheric dinitrogen fixation in diazotroph and methane production in methanogens.

Current research demonstrates the capacity for electron bifurcation in the Rhodopseudomonas palustris ETF (RpalETF) system. RpalETF contains two chemically identical but functionally different FADs: …

Evaluating And Predicting The Risk Of Algal Blooms In A Freshwater Lake Through A 4-Dimensional Approach: A Case Study On Lake Mitchell, Sumit Kumar Ghosh

Electronic Theses and Dissertations

Excessive algal growth in freshwater lakes can negatively impact ecosystems, recreation, and human health. Though algae are a natural part of freshwater ecosystems, elevated nutrient loading from anthropogenic and natural sources can lead to algal blooms. Both algae and blue-green algae (BGA) are responsible for algal blooms; however, BGA (cyanobacteria) is more dangerous. The first objective of this research was to prepare a conceptual model to understand how various environmental variables affect algae. This conceptual model was used to choose the environmental variables that help increase or decrease algae in the water environment. The second objective was to develop empirical …

Search Strategy For "Prevalence Of Adverse Childhood Experiences (Ace) Among Children And Adolescents In The U.S.: A Scoping Review", Kiara Lyons, Melissa Tibbits 5999447, Jennifer Hill, Kendra Schmid, Shinobu Watanabe-Galloway

Search Strategies: College of Public Health

No abstract provided.

Enabling Conceptual Learning To Prepare Students For The New World Of Nutrition Communication, Sabine Zempleni

UNL Faculty Course Portfolios

During the last decades, the flow of nutrition information between nutrition professionals and consumer has changed profoundly. Newest scientific findings are freely available from public data bases and often make it prematurely into the media and social media. Therefore, students need to require a precise knowledge of nutrition concepts, be able to explain the concepts to the consumer, and apply those concepts to eating decisions. Many students have not made the transition from memorizing individual facts to conceptual learning. Students will need appropriate learning tools and instructions to help them make this transition successfully. The goal of this Peer Review …

Vbms 403: Integrated Principles And Prevention Of Livestock Diseases, Christina L. Topliff

UNL Faculty Course Portfolios

This portfolio documents the teaching objectives, strategies, assessments, and changes implemented for VBMS 403: Integrated Principles and Prevention of Livestock Diseases, an Achievement-Centered Education (ACE) 10 Capstone course taught during the Spring Semester through the School of Veterinary Medicine and Biomedical Sciences at the University of Nebraska-Lincoln. Teaching methods and course activities included traditional lecturing with quizzes and examinations, in-class discussions, short reading assignments, and the generation of a scholarly term paper demonstrating broad knowledge, technical proficiency, information collection, synthesis, interpretation, and presentation. This portfolio documents the qualitative and quantitative methods used to assess the course learning objectives (goals). My …

Fdst 403/803 – Food Quality Assurance, Byron D. Chaves

UNL Faculty Course Portfolios

Food Science and Technology (FDST) 403: Food Quality Assurance is an upper level required course for FDST undergraduate majors at the University of Nebraska-Lincoln. The course covers food safety regulations, food safety management systems, and statistical process control applied to food safety and quality in food manufacturing. This Peer Review of Teaching Portfolio documents the course analysis and reflection for spring 2021. Two mid-semester student course evaluations were performed on March 1 and April 5, the lecture immediately after the midterm exams. Anonymous paper and online surveys were filled out during class time asking students to reflect on the course …

Peer Review Of Teaching Portfolio: Learning & Motivation (Psyc 368), Jacquelyn Omelian

UNL Faculty Course Portfolios

This portfolio is a reflection and report on the peer review process undertaken during the Spring 2021 semester at the University of Nebraska – Lincoln. The work presented here provides a description of the PSYC 368: Learning and Motivation course as it was delivered in an asynchronous, 14-week format as a result of the COVID-19 pandemic. This large (150 student) lecture-style course is open to any student who has completed Introduction to Psychology and attracts students from across multiple colleges and majors. This portfolio has a particular emphasis on a series of major assignments in the course and how they …

Stat 850 Course Portfolio, Susan Vanderplas

UNL Faculty Course Portfolios

Stat 850 is an introduction to statistical computing, providing students with the computing skills they need to do research, write papers, and succeed in statistics and quantitative fields. In this portfolio, I examine the impact of increased discussion of pseudocode and flow charts on students’ ability to solve multi-step problems. This additional scaffolding, while not completely effective, does seem to have resulted in lower student frustration, though it is not clear that this is a result of the intervention and not just a consequence of a different group of students with different experience in computational programming.

A Benchmark Course Portfolio For Slpa 453: Neurological Foundations Of Speech And Language, Yingying Wang Dr.

UNL Faculty Course Portfolios

This benchmark course portfolio reflects the iterative design process for SLPA 453: Neurological Foundations of Speech and Language. This course covers the foundational knowledge of neuroanatomy and neurophysiology of the central and peripheral nervous system and the neurology of the human communication processes, and the classification of impairments based on the lesion sites in the human brain. I analyzed student learning through various activities that align with two main course goals: (1) demonstrate knowledge of the neurology of human communication processes; (2) demonstrate the ability to integrate information pertaining to normal and abnormal human development across the life span. Based …

A Human Rights Approach To Risk: The Case Of Human Germline Editing, Jessica Almqvist

Loyola of Los Angeles International and Comparative Law Review

No abstract provided.

The Regulation Of Human Germline Genome Modification (Hggm) At The National Level: A Call For Comprehensive Legal Reform, Andrea Boggio, Cesare P.R. Romano, Jessica Almqvist

Loyola of Los Angeles International and Comparative Law Review

No abstract provided.

Medicinal Genome Editing In Germany – Tensions Between Safeguarding And Circumventing Ethical And Legal Standards, Timo Faltus

Loyola of Los Angeles International and Comparative Law Review

No abstract provided.

The Atr-Chek1 Pathway Inhibits Axon Regeneration In Response To Piezo-Dependent Mechanosensation, Feng Li, Tsz Y. Lo, Leann Miles, Qin Wang, Harun N. Noristani, Dan Li, Jingwen Niu, Shannon Trombley, Jessica I. Goldshteyn, Chuxi Wang, Shuchao Wang, Jingyun Qiu, Katarzyna Pogoda, Kalpana Mandal, Megan Brewster, Panteleimon Rompolas, Ye He, Pau A. Janmey, Gareth M. Thomas, Shuxin Li, Yuanquan Song

Advanced Science Research Center

Atr is a serine/threonine kinase, known to sense single-stranded DNA breaks and activate the DNA damage checkpoint by phosphorylating Chek1, which inhibits Cdc25, causing cell cycle arrest. This pathway has not been implicated in neuroregeneration. We show that in Drosophila sensory neurons removing Atr or Chek1, or overexpressing Cdc25 promotes regeneration, whereas Atr or Chek1 overexpression, or Cdc25 knockdown impedes regeneration. Inhibiting the Atr-associated checkpoint complex in neurons promotes regeneration and improves synapse/behavioral recovery after CNS injury. Independent of DNA damage, Atr responds to the mechanical stimulus elicited during regeneration, via the mechanosensitive ion channel Piezo and its downstream NO …

Vulnerable And Resilient Phenotypes In A Mouse Model Of Anorexia Nervosa, Jeff A. Beeler, Devry Mourra, Roseanna M. Zanca, Abigail Kalmbach, Celia Gellman, Benjamin Y. Klein, Rebecca Ravenelle, Peter Serrano, Holly Moore, Stephen Rayport, Susana Mingote, Nesha Burghard

Advanced Science Research Center

Background

Increased physical activity is a common feature of anorexia nervosa (AN). Although high activity levels are associated with greater risk of developing AN, particularly when combined with dieting, most individuals who diet and exercise maintain a healthy body weight. It is unclear why some individuals develop AN while most do not. A rodent model of resilience and vulnerability to AN would be valuable to research. Dopamine, which is believed to play a crucial role in AN, regulates both reward and activity and may modulate vulnerability.

Methods

Adolescent and young adult female C57BL/6N mice were tested in the activity-based anorexia …