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Effects Of Bradykinin On Proliferation, Apoptosis, And Cycle Of Glomerular Mesangial Cells Via The Tgf-Ss1/Smad Signaling Pathway, Ji Dong, Li Ding, Liuwei Wang, Zijun Yang, Yulin Wang, Ying Zang, Xuexia Cao, Lin Tang

Turkish Journal of Biology

We aimed to assess the effects of bradykinin (BK) on the proliferation, apoptosis, and cycle of glomerular mesangial cells via the transforming growth factor-ß 1 (TGF-ß1)/Smad signaling pathway. Rat glomerular mesangial cells, HBZY-1, were divided into normal group (untreated), model group (5 ng/L TGF-ß1), BK group (5 ng/L TGF-ß1 + 1 ng/L BK), and inhibitor group [5 ng/L TGF-ß1 + 1 ng/L LY2109761 (TGF-ß1-specific inhibitor)]. The cell proliferation, cycle, apoptosis, expression of type I collagen (Col-1), and protein expressions of Col-1, TGF-ß1, and phosphorylated Smad2 (p-Smad2) were detected by EdU labeling, flow cytometry, acridine orange/ethidium bromide (AO/EB) dual staining, immunofluorescence …

Increased Ribonuclease Activity In Solanum Tuberosum L. Transformed With Heterologous Genes Of Apoplastic Ribonucleases As A Putative Approach For Production Of Virus Resistant Plants, Andrii Potrokhov, Daria Sosnovska, Olga Ovcharenko, Irena Budzanivska, Volodymyr Rudas, Mykola Kuchuk

Turkish Journal of Biology

Viral pathogens seriously decrease the yield of potato (Solanum tuberosum L.) -an important agricultural crop. Therefore, there is a demand for potato cultivars resistant to multiple viruses. Ribonucleases (RNases) are supposed to be engaged to antiviral response in plants. Heterologous RNase gene expression provides a tool for production of cultivars with multiple resistance to viruses and viroids. Transgenic potato cultivars Luhivs'ka and Lasynak with heterologous genes bov and ZRNase II of apoplastic RNases from Bos taurus and Zinnia elegans respectively were obtained via Agrobacterium-mediated transformation. The presence of bov and ZRNase II transgenes was confirmed by PCR analysis. RNase activity …

An Update Comprehensive Review On The Status Of Covid-19: Vaccines, Drugs, Variants And Neurological Symptoms, Ebru Özkan Oktay, Sali̇h Tuncay, Tuğba Kaman, Ömer Faruk Karasakal, Öznur Özge Özcan, Tuğçe Soylamiş, Mesut Karahan, Muhsi̇n Konuk

Turkish Journal of Biology

Various recently reported mutant variants, candidate and urgently approved current vaccines against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), many current situations with severe neurological damage and symptoms as well as respiratory tract disorders have begun to be reported. In particular, drug, vaccine, and neutralizing monoclonal antibodies (mAbs) have been developed and are currently being evaluated in clinical trials. Here, we review lessons learned from the use of novel mutant variants of the COVID-19 virus, immunization, new drug solutions, and antibody therapies for infections. Next, we focus on the B 1.1.7, B 1.351, P.1, and B.1.617 lineages or variants of …

Neuropathy In Covid-19 Associated With Dysbiosis-Related Inflammation, Busra Aktas, Belma Aslim

Turkish Journal of Biology

Although COVID-19 affects mainly lungs with a hyperactive and imbalanced immune response, gastrointestinal and neurological symptoms such as diarrhea and neuropathic pains have been described as well in patients with COVID-19. Studies indicate that gut-lung axis maintains host homeostasis and disease development with the association of immune system, and gut microbiota is involved in the COVID-19 severity in patients with extrapulmonary conditions. Gut microbiota dysbiosis impairs the gut permeability resulting in translocation of gut microbes and their metabolites into the circulatory system and induce systemic inflammation which, in turn, can affect distal organs such as the brain. Moreover, gut microbiota …

Targeting Cov-2 Spike Rbd And Ace-2 Interaction With Flavonoids Of Anatolian Propolis By In Silico And In Vitro Studies In Terms Of Possible Covid-19 Therapeutics, Hali̇l İbrahi̇m Güler, Fulya Ay Şal, Zehra Can, Yakup Kara, Oktay Yildiz, Ali̇ Osman Beldüz, Sabri̇ye Çanakçi, Sevgi̇ Kolayli

Turkish Journal of Biology

Propolis is a multi-functional bee product rich in polyphenols. In this study, the inhibitory effect of Anatolian propolis against SARS-coronavirus-2 (SARS-CoV-2) was investigated in vitro and in silico. Raw and commercial propolis samples were used, and both samples were found to be rich in caffeic acid, p-coumaric acid, ferulic acid, t-cinnamic acid, hesperetin, chrysin, pinocembrin, and caffeic acid phenethyl ester (CAPE) at HPLC-UV analysis. Ethanolic propolis extracts (EPE) were used in the ELISA screening test against the spike S1 protein (SARS-CoV-2): ACE-2 interaction for in vitro study. The binding energy values of these polyphenols to the SARS-CoV-2 spike and ACE-2 …

The Potential Use Of Drosophila As An In Vivo Model Organism For Covid-19-Related Research: A Review, Eşref Demi̇r

Turkish Journal of Biology

The world urgently needs effective antiviral approaches against emerging viruses, as shown by the coronavirus disease 2019 (COVID-19) pandemic, which has become an exponentially growing health crisis. Scientists from diverse backgrounds have directed their efforts towards identifying key features of SARS-CoV-2 and clinical manifestations of COVID-19 infection. Reports of more transmissible variants of SARS-CoV-2 also raise concerns over the possibility of an explosive trajectory of the pandemic, so scientific attention should focus on developing new weapons to help win the fight against coronaviruses that may undergo further mutations in the future. Drosophila melanogaster offers a powerful and potential in vivo …

Determination Of The Interaction Between The Receptor Binding Domain Of 2019-Ncov Spike Protein, Tmprss2, Cathepsin B And Cathepsin L And Glycosidic And Aglycon Forms Of Some Flavonols, Erman Sali̇h İsti̇fli̇, Arzuhan Şihoğlu Tepe, Paulo Augusto Netz, Cengi̇z Sarikürkcü, İbrahi̇m Hali̇l Kiliç, Bektaş Tepe

Turkish Journal of Biology

The novel coronavirus (COVID-19, SARS-CoV-2) is a rapidly spreading disease with a high mortality. In this research, the interactions between specific flavonols and the 2019-nCoV receptor binding domain (RBD), transmembrane protease, serine 2 (TMPRSS2), and cathepsins (CatB and CatL) were analyzed. According to the relative binding capacity index (RBCI) calculated based on the free energy of binding and calculated inhibition constants, it was determined that robinin (ROB) and gossypetin (GOS) were the most effective flavonols on all targets. While the binding free energy of ROB with the spike glycoprotein RBD, TMPRSS2, CatB, and CatL were -5.02, -7.57, -10.10, and -6.11 …

Cannabinoids For Sars-Cov-2 And Is There Evidence Of Their Therapeutic Efficacy?, Ahmet Onay, Abdulselam Ertaş, Veysel Süzerer, İsmai̇l Yener, Mustafa Abdullah Yilmaz, Emi̇ne Ayaz Ti̇lkat, Remzi̇ Eki̇nci̇, Nesri̇n Bozhan, Sevgi̇ İrtegün

Turkish Journal of Biology

To combat the coronaviruses and their novel variants, therapeutic drugs and the development of vaccines that are to be effective throughout human life are urgently needed. The endocannabinoid system (ECS) acts as a modulator in the activation of the microcirculation, immune system, and autonomic nervous system, along with controlling pharmacological functions such as emotional responses, homeostasis, motor functions, cognition, and motivation. The ECS contains endogenous cannabinoids, cannabinoid receptor (CBRs), and enzymes that regulate their biosynthesis, transport, and degradation. Moreover, phytocannabinoids and synthetic cannabinoids that mimic the action of endocannabinoids also play an essential role in the modulation of the ECS. …

Predatory Microtransaction Regulations: An International Comparison, Matt Hardy

South Carolina Journal of International Law and Business

No abstract provided.

Bangladesh-Bhutan Pta: Less Is More?, Md. Rizwanul Islam

South Carolina Journal of International Law and Business

While in the General Agreement on Tariffs and Trade (GATT) years, generally developed economies pursued preferential trade agreements (PTAs), and the states of the global South were also pursuing PTAs between and among each other. The recently concluded Bangladesh-Bhutan Preferential Trade Agreement (BBPTA) is an addition to this list. This article argues that the seemingly narrow scope of the BBPTA is somewhat deceptive. This is so because it covers products which are actually traded between the parties, and they create a substantial margin of preference. Thus, though the relatively liberal and simple rules of origin of the BBPTA is a …

Effects Of Harvest Regulations And Post-Release Hooking Mortality On Walleye Populations In South Dakota, Cade Lyon

Electronic Theses and Dissertations

Harvest regulations are commonly implemented to manipulate fisheries stocks. By regulating the size and number of fish that are harvested by anglers, managers are able to meet the goals and needs of regions. However, these management actions come with the potential for negative consequences. Overexploitation due to less restrictive harvest regulations can cause collapses in fisheries populations. In addition, indirect consequences such as hooking mortality brought on by length-based regulations can also be detrimental to populations. In this study, I investigated the effects of various harvest regulations on Walleye populations in three western South Dakota irrigation reservoirs: Angostura, Belle Fourche, …

Military Combat Veterans, Sentencing Reform, And Problem-Solving Courts, Andrea Sivanich

Theses and Dissertations

Military veterans involved with the criminal justice system may have unique behavioral health and physical needs, including post-traumatic stress disorder (PTSD) or traumatic brain injury (TBI), and could benefit from sentence mitigation that emphasizes rehabilitation. This research is related to sentencing reform and how it may support problem-solving court sentencing, as a subset of community-based treatment options within the criminal justice system. This research is important in that it attempts to determine whether the law distinguishes veteran offenders, as a specific population, from other offenders with treatment needs. To do that, this dissertation examines the role of legal texts in …

Legal Marijuana Disperser Owners' Lived Experiences About Accessing Banking Services, Jonathan Travis James

Walden Dissertations and Doctoral Studies

Marijuana reform has created a question of the potential market size of the legal marijuana industry on a local, state, or national level. The problem is that having no banking services has forced legal marijuana dispensaries to be cash-only businesses and created an increased risk of theft. The lack of normal banking services has also affected the ability to manage money earned from operations. This hermeneutic phenomenological study was grounded by the conceptual framework of the motivations of humans and humans' need to feel safe. The participants of this study were owners and operators in the legal marijuana industry of …

Social Support Of Caregiver Sons Of Parents With Dementia, Ralph Callanta Pascual

Walden Dissertations and Doctoral Studies

Despite the growing number of male caregivers, few researchers have examined social supports for caregiver sons of parents with dementia. The purpose of this qualitative interpretative phenomenological study was to contribute to the empirical literature on the social support of caregiver sons of aging parents with dementia. The research question centered on the lived experience of social support of caregiver sons of parents with dementia. The theoretical framework was the social role theory. The sample consisted of eight caregiver sons of parents with dementia. Participants were interviewed using semi-structured, researcher-developed questions. The results were generated from coding and thematizing processes, …

Funding Strategies For Nonprofit Animal Shelter Leaders, Destiny Reeder

Walden Dissertations and Doctoral Studies

Nonprofit animal shelters provide vital services to the community and improve the overall well-being of animals. Many animal shelters, however, face the challenge of insufficient funding. Some nonprofit animal shelter leaders are burdened with financial uncertainty because of a lack of funding strategies for operational stability and survival. Grounded in the resource dependency theory, the purpose of this qualitative multiple case study was to explore strategies that nonprofit animal shelter leaders use to obtain funding. The participants comprised three nonprofit animal shelter leaders in Maryland. Data were collected from semistructured telephone interviews and public financial reports. Data analysis was conducted …

Perceptions Of Using Complementary Alternative Medicine During Pregnancy For Nausea And Vomiting, Debra L. Blackett

Walden Dissertations and Doctoral Studies

Nausea Vomiting during Pregnancy (NVP) conditions have commonly occurred among pregnant women during gestation for more than 4,000 years. The purpose of the multicase qualitative design was to examine and improve the understanding of perceptions of pregnant women experiencing nausea and the potential use of Complementary Alternative Medicine (CAM) modalities to treat NVP symptoms. In this study, the Health Belief Model six core constructs provided the foundation for the theoretical framework for the process, explaining how working pregnant women experience decision-making and relate to the daily challenges associated with pregnancy. The research questions examined experience of coping with NVP and …

Reducing Produced Water Disposal Via Effective Treatments Methods And Re-Use: Proposed Sustainable Application For Bakken, North Dakota, Olusegun Stanley Tomomewo

Theses and Dissertations

It is true that the advancements in both the hydraulic frack and directional drilling technologies led to less time and a bit easier ways to develop unconventional oil and gas assets worldwide. In the Bakken North Dakota, the result of these breakthroughs and advancements in technologies are that they drastically reduce the time it takes to drill and complete a well leading to more wells (347 in 2004 to 16,300 in 2020). In 2019, the United States became the largest global crude oil producer, and the unconventional Bakken Play in North Dakota is one of the major contributors to this …

Using Machine Learning On Diverse Datasets To Predict Drug-Induced Liver Injury, Temidayo Adeluwa

Theses and Dissertations

A major challenge in drug development is safety and toxicity concerns due to drug sideeffects. One such side effect, drug-induced liver injury (DILI), is considered a primary factor in regulatory clearance. To develop prediction models of DILI, the Critical Assessment of Massive Data Analysis (CAMDA) 2020 CMap Drug Safety Challenge goal was established with an ultimate goal to develop prediction models based on gene perturbation of six preselected cell-lines (CMap L1000), extended structural information (MOLD2), toxicity data (TOX21), and FDA reporting of adverse events (FAERS). Four types of DILI classes were targeted, including two clinically relevant scores and two control …

Endolysosome Involvement In The Protective Effects Of 17Ơ-Estradiol Against Hiv-1 Protein-Induced Neuronal Injury, Nicole Miller

Theses and Dissertations

Although combined antiretroviral therapy (cART) effectively suppresses HIV-1 infection and increases the lifespan of people living with HIV, up to 50% of HIVinfected individuals are experiencing HIV-associated neurocognitive disorder (HAND), with clinical symptoms ranging from mild cognitive impairment to severe dementia, for which no effective treatment is currently available. Synaptodendritic impairments, such as decreased dendritic spines and synaptic loss, that occur in various brain regions including the prefrontal cortex and hippocampus are key pathological features of HAND that correlates closely with neurocognitive impairments. The development of synaptodendritic impairment in HAND in the post-cART era is complicated and not fully understood; …

Bubonic Plague: Public Health Warnings From An Ancient Disease, Mikenzie E. Chessman

2022 Award Winners

No abstract provided.

The Tijeras Pueblo (La 581) Archaeofaunal Project, Emily Lena Jones, Scott Kirk, Caitlin S. Ainsworth, Asia Alsgaard, Jana Valesca Meyer, Cyler Conrad

Anthropology Datasets

These files contain data generated by the Tijeras Pueblo (LA 581) Archaeofaunal Project, a project of the University of New Mexico Department of Anthropology Zooarchaeology Laboratory between 2011 and the present. This project has been supported in part by the National Science Foundation under Grant No. 1732622 and by a grant from the Research Allocations Committee of the University of New Mexico.

These data are the basis of the analyses presented in the following publication:

Jones, Emily Lena, Scott Kirk, Caitlin S. Ainsworth, Asia Alsgaard, Jana Valesca Meyer, and Cyler Conrad. 2021. The Community at the Crossroads: Artiodactyl Exploitation and …

"When They See Us" The Great White Awakening To Black Humanity, Gregory S. Parks

University of Maryland Law Journal of Race, Religion, Gender and Class

No abstract provided.

Overlapping Roles Of Nadph Oxidase 4 (Nox4) For Diabetic And Gadolinium-Based Contrast Agent-Induced Systemic Fibrosis-Animal Equivalent Dosing Of Gadolinium-Based Contrast Agents Supplementary Figure 2, Francesca Bruno, Joshua Deaguero, Catherine Do, Doug Yoon Lee, Chunyan Tan, G Patricia Escobar, Brent Wagner

The Kidney Institute of New Mexico

Supplementary figure for the forthcoming publication entitled “Overlapping roles of NADPH Oxidase 4 (Nox4) for diabetic and gadolinium-based contrast agent-induced systemic fibrosis”. Supplementary Figure 2 demonstrates renal changes induced by gadolinium-based contrast agent treatment and experimental diabetes. Photomicrographs analyzed by a blinded veterinary pathologist.

Nature’S Personhood And Property’S Virtues, Laura Spitz, Eduardo M. Peñalver

Faculty Scholarship

This Article evaluates the strategy of claiming personhood for natural objects as a way to advance environmental goals in the United States. Using the Colorado River Ecosystem v. Colorado litigation as the focus, we explore the normative foundation of the claim—elements of nature are legal persons—and the work personhood is being asked to do by the plaintiff and other environmental activists. We identify three possibilities: procedural work, substantive work, and rhetorical work. Of those, we suggest the plaintiff’s strongest case is rhetorical. We say this not only because it will likely be difficult to convince a judge to extend standing …

Effects Of Neuronal Activation And Psychiatric Treatment On Circhomer1 Biogenesis, Grigorios Papageorgiou, Nikolaos Mellios

Brain & Behavioral Health Research Day

Schizophrenia (SCZ) and Bipolar disorder (BD) are heterogeneous psychiatric disorders that together affect more than 3.5% of the US population. Studies using animal, pharmacological models

and post-mortem data, have revealed abnormalities in the dopaminergic and glutamatergic pathways, however, the pathogenesis remains vague. Non-coding RNAs (ncRNAs) have been shown to play a role in regulating gene expression at the transcriptional and post-transcriptional level and having implications on brain development and psychiatric diseases. Circular RNAs are a category of ncRNAs, formed after back-splicing of exons/introns, covalently linked, enriched in the mammalian brain and derived from genes that are implicated in synaptic plasticity. …

Prenatal Alcohol Exposure Enhances Morphine-Mediated Toll Like Receptor (Tlr)-4 Actions And Paradoxically Prolongs Nerve Injury-Induced Pathological Pain, Shahani Noor, Melody S. Sun, Chaselyn D. Ruffaner-Hanson, Monique V. Nysus, Diane C. Jimenez, Fernando F. Valenzuela, Erin D. Milligan

Brain & Behavioral Health Research Day

Our prior work demonstrates that adult prenatal alcohol exposed (PAE) rats with minimal nerve injury develop allodynia (pathological light touch sensitivity) in concert with aberrant peripheral-spinal glial and immune activation. Nerve-injured PAE rats display heightened proinflammatory cytokine production (IL-1β, TNF-α, CCL2) typically produced following activation of innate immune receptors, TLR4 and Nod-like receptor family pyrin domain containing 3 (NLRP3) inflammasomes. This suggests that PAE-related adaptations prime peripheral macrophages and central nervous system (CNS) glia to over-respond to subtle challenges through exaggerated TLR4 and NLRP3 inflammasome reactivity. Interestingly, morphine, a standard opioid pain therapeutic, binds the μ-opioid receptor and also glial …

Women Lawyers For Social Causes, Frank W. Munger, Peerawich Thoviriyavej, Vorapitchaya Rabiablok

Articles & Chapters

Women lawyers are increasing seen among the leading legal defenders of human rights and social movements in Thailand. Increasing visibility is partly a result of news coverage and social media, but women lawyers activism has far older roots. In this article, we examine two related processes of change that contribute to women’s emergence as leading social cause practitioners. First, we discuss the relationship between Thailand’s legal system and its social and political development since the end of the nineteenth century. Second, we employ career narratives of three women lawyers with innovative practices for social causes as a lens through which …

The Road To Free-Market Family Policy, Maxine Eichner

Faculty Publications

This essay investigates and ultimately rejects the claim that the United States’ comparatively heavily reliance on markets over government to provide the resources that families need is a natural outgrowth of the country’s longstanding veneration of capitalism. In tracing the nation’s economic ideology over time, it demonstrates that, at least until the end of the twentieth century, the constant in US history has not been the expectation of a free-market economy, but rather a commitment to ensuring that the economy, however structured, will enable families to thrive. The dramatic recent shift in economic ideology, when the prevailing commitment to government …

Standing And Contracts, F. Andrew Hessick

Faculty Publications

In Spokeo v. Robbins, the Supreme Court held that, to establish Article III standing to bring suit in federal court, a plaintiff cannot simply allege the violation of a legal right. Instead, the plaintiff must allege an injury in fact. Although it addressed standing to bring suit for statutory violations, Spokeo raises serious questions about limits on the ability to bring breach of contract actions in federal court. After all, contracts simply create legal rights. Under Spokeo’s logic, the breach of contractual rights should not support standing; instead, standing exists only if the breach results in factual harm. …

Nuts And Bolts: Getting Started In Farming Checklist, Kelly Mcadam, Extension Community & Economic Development

UNH Cooperative Extension

No abstract provided.