Digital Commons Network^™

Role Of Nurd In Acute Leukemia Cell Survival, Javeria Aijaz

Theses and Dissertations

Depletion of the ATPase component of the Nucleosome Remodeling and Deacetylase (NuRD) complex, CHD4, reduces acute myeloid leukemia (AML) cell survival. This study identified other NuRD components, as potential therapeutic targets for disrupting protein-protein interactions within NuRD. In addition to AML, we established that T-cell Acute Lymphoblastic Leukemia (T-ALL) cell lines responded similarly to CHD4 depletion.

Greater than 90% depletion of either MBD2 or MBD3 (the mutually exclusive two DNA binding NuRD paralogues) – was unremarkable, but complete depletion of MBD3 increased apoptosis and genotoxic sensitivity. Combined depletion of MBD-NuRD proteins augmented apoptosis observed with complete MBD3 depletion - indicating …

Teacher Approaches To Women’S History, Gender, And Feminism In Secondary Social Studies, Kimberly R. Bowman

Theses and Dissertations

While several scholars have worked to reimagine social studies curriculum from a feminist perspective, there are few studies that look at how teachers understand and experience gender equity work (Bohan, 2017; Engebretson, 2018). This study seeks to bridge that gap between theory and practice in order to progress toward a more gender-equitable social studies education. Utilizing a hermeneutic circle design to facilitate dialogue across participants, I conducted one-on-one, semi-structured interviews with seven secondary social studies teacher participants and collected participant-provided curriculum materials. Using techniques from constructivist grounded theory, I analyzed how teachers made sense of their experiences trying to include …

An Intersectional Analysis Of Disproportionality Of Dual Language Learners In Special Education In Virginia: A Mixed Methods Study, Melissa J. Cuba

Theses and Dissertations

The disproportionality of dual language learners (DLLs) in special education has been a persistent and complex issue for decades. These students have multidimensional identities that require a look at how they are positioned in school systems and the broader social landscape. Using a multilevel model of intersectionality and an explanatory mixed methods design, this study examines how social categories, practice, and policies influence the representation of DLLs in special education in Virginia, a state where DLLs represented 13 percent of the total student population in 2018-19. Findings from this study reveal overrepresentation and underrepresentation of 63 DLL subgroups using nuanced …

“Is Therapy For Me?” Perceptions Of Therapy Inclusivity And Willingness To Seek Help Among Black Emerging Adults, Randl B. Dent

Theses and Dissertations

Mental health issues are prevalent among Black emerging adults; however, they tend to underutilize mental healthcare services. The goals of the current study were to examine whether: (1) perceived therapy inclusivity would predict willingness to utilize mental healthcare services and (2) the relationship between perceived therapy inclusivity and willingness would be moderated by two indices of racial identity (i.e., centrality and private regard). Results provide evidence that greater perceptions of therapy inclusivity are associated with greater willingness to seek mental health services even after controlling for factors, such as gender, self-stigma, and previous mental healthcare utilization. Additionally, there was no …

Characterization Of The Tsc/Dyrk1a Interaction, Supriya Joshi

Theses and Dissertations

The Tuberous sclerosis complex (TSC) includes TSC1, TSC2 and the TBC1D7 subunits that together function as a principal inhibitor of the mTOR protein kinase complex 1 (mTORC1). mTORC1 is a master regulator of cell growth and proliferation that responds to signaling cues such as growth factors and nutrient availability. Proteomic studies in our lab revealed an interaction between the TSC subunits and DYRK1A, a ubiquitous protein kinase encoded by a gene located in the Down syndrome (DS) region on human chr21. In this study, we sought to validate the interaction of the TSC components with DYRK1A and to determine the …

A Randomized Trial Of Cbt4cbt For Women In Residential Treatment For Substance Use Disorders, Sydney S. Kelpin

Theses and Dissertations

Women with substance use disorders (SUD) face unique barriers to substance use treatment, and as a result, are less likely than their male counterparts to seek treatment for the disorder. Cognitive behavioral therapy (CBT) is an evidence-based treatment known to reduce relapse rates by teaching clients to recognize and respond to their cues for substance use. Recent research suggests CBT may be particularly of benefit to women. Despite the effectiveness of CBT, its dissemination in clinical practice is limited due to a range of barriers (e.g., time, cost). Computer-based training for cognitive behavioral therapy (CBT4CBT) offers an opportunity to improve …

Disentangling Student Engagement In Afterschool Programs, Ashlee M. Lester

Theses and Dissertations

This dissertation presents a line of research exploring the characteristics and role of student engagement in afterschool programs, specifically during early adolescence. The dissertation takes a two-study format, building off of my comprehensive exam Measuring engagement in out-of-school time programs. This first chapter is an introduction in which I will detail the underlying problem that is investigated in the following two manuscripts. Following this introduction, the two separate studies are presented.

Higher Tensile Forces Across Cellular Junctions And An Intact Nuclear Linc Complex Is Required For Epithelial Function And Stability, Fnu Vani Narayanan

Theses and Dissertations

Recent advances in three-dimensional (3D) cell culture systems have provided key insights into the understanding of biochemical and physiological states of native tissue. A significant progress in the field of mechanobiology involves measuring cellular traction forces in a more native 3D environment. However, the effects of mechanical forces exerted across cellular junctions and the nuclear LINC complex, in an organized 3D system has not been investigated thus far. Epithelial cells spontaneously form acini (also known as cysts or spheroids) with a single, fluid-filled central lumen, when grown in 3D matrices. The size of the lumen is dependent on apical secretion …

Phenotype Extraction: Estimation And Biometrical Genetic Analysis Of Individual Dynamics, Kevin L. Mckee

Theses and Dissertations

Within-person data can exhibit a virtually limitless variety of statistical patterns, but it can be difficult to distinguish meaningful features from statistical artifacts. Studies of complex traits have previously used genetic signals like twin-based heritability to distinguish between the two. This dissertation is a collection of studies applying state-space modeling to conceptualize and estimate novel phenotypic constructs for use in psychiatric research and further biometrical genetic analysis. The aims are to: (1) relate control theoretic concepts to health-related phenotypes; (2) design statistical models that formally define those phenotypes; (3) estimate individual phenotypic values from time series data; (4) consider hierarchical …

Effects Of Behavioral Skills Training With In Situ Training On Workplace Conversational Skills Of Transition-Aged High School Students With Autism Spectrum Disorder, Holly N. Whittenburg

Theses and Dissertations

Young adults with autism spectrum disorder (ASD) experience high rates of unemployment in the years immediately following high school (Hendricks & Wehman, 2009; Roux et al., 2015), yet very few studies to date have investigated approaches to teaching transition-aged high school students work-related social skills within competitive, integrated workplace settings. Furthermore, difficulties in developing relationships with coworkers within the workplace may negatively affect employment (Baldwin et al., 2014; Sperry & Mesibov, 2005). Research is needed that examines methods for improving interactions with coworkers.

This study investigated the effects of a behavioral skills training (BST) with in situ training intervention package …

Comparison Of Endodontic Treatment Planning Decisions Using Cone Beam Computed Tomography Versus Periapical Radiography In Previously Endodontically Treated Molars, Sarah Alkanderi

Theses and Dissertations

Objectives: The aim of this study was to determine if treatment plans differ when evaluating previously endodontically treated molars using periapical images (PA’s) alone versus PA’s and CBCT.

Methods: A retrospective cohort chart review was conducted including 67 previously treated molars that had a CBCT taken. Two calibrated board-certified endodontists evaluated standard PA’s. A data sheet questionnaire regarding radiographic findings and treatment plan decisions was completed. Two weeks later, the same steps were repeated but the examiners evaluated both PA’s and CBCT images and again arrived at a recommended treatment plan.

Results: Agreement between raters was assessed with Cohen’s Kappa …

A Meisner Warm-Up: Advancing The Technique, Andrew Bryce

Theses and Dissertations

Why is there no warm-up for the Meisner technique? Why do standard vocal warm-ups not actually prepare the actor for performance? This thesis seeks to remedy both of these issues in 21st century actor training. It does so by creating a warm-up for the Meisner technique that will also prepare actors’ voice, body, and presence. This warm-up was formed by combining Meisner repetition exercises and the Miller Voice Method. The final warm-up was presented as a workshop for actors at the 2020 Southeastern Theatre Conference.

Senescent Trees Stabilize Aboveground Wood Net Primary Production Immediately After Disturbance, Maxim S. Grigri

Theses and Dissertations

In the United States, forests sequester 17% of national carbon (C) emissions annually (UGCRP, 2018), however shifting forest disturbances threaten the stability of this essential C sink. Unlike the high severity, stand-replacing disturbances that were widespread a century ago, today’s eastern temperate forests experience frequent low-to-moderate severity disturbances from invasive pests and pathogens with mixed effects on net primary production (NPP). Carbon cycling stability after disturbance has been reported, however, the mechanisms underlying immediate NPP stability or decline are not well understood. Through weekly measurements of production in a landscape scale experiment, we show that the sustained growth of senescent …

Dna Analysis And The Postmortem Submersion Interval From The Microbiome Of Waterlogged Sus Scrofa Skeletal Remains, Claire Cartozzo

Theses and Dissertations

Following water-related deaths, soft tissue on victims’ remains may be entirely absent after a temperature dependent period of time. Due to their physical and chemical components, which protect them from environmental deterioration and biological attack, bones preserve DNA for longer periods of time. Therefore, the ability to both identify an individual and estimate the postmortem submersion interval (PMSI) may depend on DNA obtained from skeletal remains. The goals of the dissertation were to examine differences in both the degradation of skeletal DNA and the microbiome inhabiting bone in freshwater aquatic environments (e.g., Henley Lake and the James River). At each …

Magnetic Refrigerator Device Based On La (Fexcoysi1-X-Y)13, Khushar Javed

Theses and Dissertations

Particles of Lanthanum-iron-silicide, La (Fe_xCo_ySi_1-x-y)₁₃exhibit different magnetic properties when stored in air compared to the same stored in water. The sample with a nominal composition of La(Fe0_.842Co_0.073Si_0.084)₁₃was prepared by arc-melting from elements under argon atmosphere. In recent years, there has been research conducted on different processes that could drastically reduce energy costs of cooling and replacing gas compressors with magnetocaloric materials. Magnetization measurements were carried out on this magnetocaloric material to characterize it in order to design magnetic refrigerator device based on it. The …

Characteristics Of Alveolar Bone Marrow Cells From Patients Undergoing Dental Extractions Or Dental Implant Therapy, Meng H. Lee

Theses and Dissertations

Alveolar bone marrow stromal cells (aBMSCs) play important roles in craniofacial wound healing. To establish an easy, efficient and reliable method to harvest aBMSCs, we compared three different methods: extraction socket aspiration, osteotomy aspiration and bone core digestion. Samples of aBMSC were collected from two groups of subjects. Group 1 (dental extraction): after dental extraction, 22.5-gauge needles were used to collect 0.5-1cc marrow aspirate. Group 2 (dental implant): during implant surgeries, bone core and 0.5-1cc marrow aspirate were obtained from the osteotomy. Samples were cultured in petri dishes and attached cells were expanded. The population doubling time (PDT), surface markers, …

Predictors Of Mental Health Need And Treatment In Safety Net Primary Care, Kristen O'Loughlin

Theses and Dissertations

Many mental health (MH) needs go unidentified in primary care, and certain patients appear to be at higher risk of needs going unidentified and subsequently untreated. Little is known about patterns of detection and treatment in clinics with integrated mental health services. The purpose of this study was to characterize the prevalence of MH needs and evaluate patient characteristics as predictors of both the presence of a MH need and type of MH services received. Subjects were patients receiving care at two safety net primary care clinics with integrated mental health services (N=816; 52.7% Latinx, 15.9% African American), …

The Future In The Instant: Time As The Essence Of Dramatic Tension In Shakespeare's Plays And Their Contemporary Stage Appropriations, Amalia Oswald

Theses and Dissertations

This project looks at the similarities and differences between three Shakespeare plays and their companion contemporary stage appropriations: Othello and Toni Morrisons’s Desdemona, The Winter’s Tale and Mary Elizabeth Hamilton’s 16 Winters or the Bear’s Tale, and Macbeth and Daivd Grieg’s Dunsinane. The specific lens used to examine these texts is the dramatic concept of time, taking the form of different variations throughout the work such as chronology, remembrance, fate, and futurity. This project argues that the multifaceted dramatic concept of time is the foundation for dramatic tension and the principal connecting motif between the three sets of early modern/contemporary …

Associations Between Stress, Racial Discrimination, And Cytokine Levels In Black Americans, Takia Williams

Theses and Dissertations

Inflammation is a common pathophysiological pathway for a number of chronic diseases and is influenced by exposure to stress. Although there are racial disparities in health outcomes, relatively little is known about factors that may influence the inflammatory response in Black American individuals. This study examined whether racial discrimination and other forms of stress are associated with the balance of pro- and anti- inflammatory cytokines in Black American adults. Data from 22 participants were drawn from a larger study of Black American children (ages 5-12) and their primary caregivers drawn from low income neighborhoods in Richmond, Virginia. Caregivers reported demographics, …

Development Of Near-Real Time Material Detection And Analysis By Coupling Electrochemical & Optical Spectroscopy Methods For Molten Salt Systems, Hunter Andrews

Theses and Dissertations

For both pyroprocessing and molten salt reactors (MSRs), it is desirable from a process control and nuclear safeguards perspective to monitor the species in the molten salts for material accountancy. Many traditional monitoring techniques, even simple salt sample retrieval, are time exhaustive or rendered useless for these systems due to the high thermal loads, high radiation zones, and corrosive nature of molten salts. Therefore, the main motivation for this work is the need for an online monitoring system for a molten salt system that is robust enough to handle the extreme environment yet deliver quantifiable salt concentrations. The intent of …

Characterizing Glycosaminoglycan And Glycosaminoglycan-Oligo Interactions With Cancer Stem Cells And Their Regulators, Rahaman Navaz Gangji

Theses and Dissertations

Tumor metastasis and recurrence account for more than 90% of cancer-associated deaths and are thought to occur due to a small subpopulation of neoplastic cells known as cancer stem cells (CSCs). Because CSCs are resistant to conventional chemotherapy, there is a critical need to develop CSC-targeting agents. Microenvironmental components, especially glycosaminoglycans (GAGs), play a key role in regulating CSC phenotype. GAGs such as heparan sulfate (HS) interact with hundreds of proteins and influence a variety of biological processes including tumor proliferation, angiogenesis, metastasis and stem cell biology.

Recently, the Desai and Patel laboratories have demonstrated that a hexasaccharide form of …

The Relationships Among External Environments, Organizational Structures, And Performance In Pursuit Of The Clinical And Academic Missions At Academic Health Centers, Eric J. Strucko

Theses and Dissertations

Clinical and academic missions place Academic Health Centers (AHCs) at the center of the American health care system, and the future viability of these institutions requires successful performance in competitive environments. AHCs are organizations involving hospitals, physician group practices, and medical schools, and treat patients with complex conditions, conduct bio-medical and health science research, and educate future physicians and health professionals. AHCs account for 20% or $540 billion of national health care expenditures, conduct over 80% of all heart, liver, and lung transplants, utilize over $27 billion in annual sponsored research funding, and graduate approximately 17,300 medical doctors annually. Financial …

Psychometric Developments Of The Ace-Iq: Understanding The Trauma History Of Latine Immigrants, Jorli K. Swingen Dr.

Theses and Dissertations

Adverse childhood experiences (ACEs) have a profound effect on an individual’s physical and mental health. The World Health Organization has recently updated the ACE questionnaire so it could be used with international populations. The Adverse Childhood Experiences-International Questionnaire (ACE-IQ), has not been translated to Spanish or used with Latine immigrants.

This study translated the ACE-IQ into Spanish, evaluated the psychometric properties of the questionnaire, and collected data on 184 four adult English Language Learners in Chesterfield County, VA.

There is evidence of internal consistency for the ACE-IQ as a whole (α = .908) and within subscales. Three factors were identified …

Teachers' Perceptions Of The Broad Validity Of A High Stakes English Language Proficiency Test, Carolyn N. Waters

Theses and Dissertations

The No Child Left Behind Act of 2001 mandated that the English acquisition of all students identified as English learners be assessed annually using high-stakes standardized English language proficiency tests, and the Every Student Succeeds Act of 2015 continues this testing mandate. The WIDA ACCESS for ELLs® has been used for this and other purposes since 2005, and has been adopted by 40 State Education Agencies, including the Virginia Department of Education. However, despite the long-standing and widespread use of this assessment, no comprehensive independent evaluations of the test have been conducted. This mixed-methods study is a conceptual replication of …

Modulation Of Ferroportin In Oral Cancer, Ashley N. Hemingway

Theses and Dissertations

Iron plays a critical role in life sustaining functions for the cell. Dysregulation of these processes can be an issue with maintaining homeostatic conditions. Various cancer types have been observed to show malfunction in the maintenance of their iron homeostasis, in particular with the iron export protein—ferroportin. Ferroportin is the sole iron exporter known. Its structure and mechanism have not fully been elucidated yet, but its modulation has been associated with a poorer prognosis in cancers like breast, leukemia, prostrate, ovarian, colorectal, etc. Decreased iron exportation by ferroportin may lead to an increased labile iron pool allowing the cell to …

A Comparison Of Patient Testimonials On Youtube Of The Most Common Orthodontic Treatment Modalities: Braces, In-Office Aligners, And Direct-To-Consumer Aligners, Riley Hunsaker

Theses and Dissertations

Introduction: The objectives of this research was to investigate and compare the educational value of the most popular YouTube orthodontic patient testimonials between braces (B), in- office aligners (IOA), and direct-to-consumer aligners (DTCA), and to classify the emotional response of the viewers through a sentiment analysis of the video comments.

Methods: Three different phrases relevant to B, IOA, and DTCA were searched on YouTube. The 20 most popular patient testimonial videos that met the criteria for each group were selected, for a total of 60 videos. Using the YouTube API for each video, 13 video metrics were extracted, an information …

Design, Synthesis, And Biological Evaluation Of Mu Opioid Receptor Selective Antagonists Bearing Pyrazole And Imidazole Side Chains, Pornprom Klongkumnuankarn

Theses and Dissertations

Opioid Use Disorder (OUD) is one of the major national threats to human health and economic system. Since the opiate substances produce their pharmacological effects mainly at the Mu Opioid Receptor (MOR), the development of potent MOR selective antagonists is imperative. By application of the message-address concept, the novel selective MOR antagonist, NAP was synthesized in our group. The molecular modeling studies of NAP revealed that its MOR selectivity was determined by interactions between the C(6) side chain and the non-conserved residues of the receptor. To enrich our understanding of Structure-Activity Relationship (SAR) of the opioid ligands as well as …

The Design Of A New Vaginal Applicator For Direction Modulated Brachytherapy (Dmbt) Using Geant4 Monte Carlo Simulation Code, Moeen Meftahi

Theses and Dissertations

Purpose: Anisotropy of the 192Ir source causes a lack of dose coverage at the apex in HDR vaginal cylinder brachytherapy (VC BT). In this study, we took advantage of the GEANT4 Monte Carlo (MC) simulation code to design a new DMBT vaginal applicator so that it maintains the dose coverage of the current vaginal cylinder at periphery everywhere while improving the apex coverage. In addition, since current treatment planning systems (TPS) considers the whole environment as water according to TG43 formalism, based on the capability of the MC in the calculation of dose in the material, the effect of inhomogeneity …

"Island Of Integration": Desegregation Of The Women's Army Corps At Fort Lee, Virginia, 1948-1954, Meika Downey

Theses and Dissertations

Countless studies exist examining President Harry S. Truman’s Executive Order 9981 mandating racial desegregation of the U.S. armed forces, though all singularly focus on the experiences of male soldiers in the twentieth century. This thesis examines how the Women’s Army Corps (WAC) training center at Fort Lee, Virginia implemented desegregation in 1950 in the midst of the Korean War with relative speed and tolerance. Determined through archival records including official WAC reports, photographs, newspapers, and nine newly conducted racially diverse oral history interviews with WAC veterans, I demonstrate how the Fort Lee training center became a physical and cultural “island …

The Effect Of Print Angulation On The Accuracy And Precision Of 3d-Printed Orthodontic Retainers, Allison Williams

Theses and Dissertations

Purpose: The aims of this study were: 1) to compare the accuracy and precision of 3-

dimensional (3D) printed retainers at various angulations, 2) to evaluate the effect of angulation

on printing time and the amount of resin consumed.

Methods: Using a stereolithography (SLA) 3D printer, a total of 60 clear retainers were printed at five angulations (n=12, each): 15, 30, 45, 60, and 90 degrees. Six retainers were printed each cycle at a random order for all print angulations as print 1 and print 2. Digital images of the original and printed retainers were superimposed. Discrepancies on eight landmarks …