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2020

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Articles 9931 - 9960 of 32283

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A Validated Hplc Method For The Simultaneous Determination Of Vincamine And Its Potential Degradant (Metabolite), Vincaminic Acid: Applications To Pharmaceutical Formulations And Pharmacokinetic Studies, S.M. Sabry, T.S. Belal, M.H. Barary, M.E.A.-L. Ibrahim Jul 2020

A Validated Hplc Method For The Simultaneous Determination Of Vincamine And Its Potential Degradant (Metabolite), Vincaminic Acid: Applications To Pharmaceutical Formulations And Pharmacokinetic Studies, S.M. Sabry, T.S. Belal, M.H. Barary, M.E.A.-L. Ibrahim

Journal of Food and Drug Analysis

A sensitive and specific HPLC method was developed and validated for the simultaneous determination of vincamine and its potential degradant (metabolite), vincaminic acid. Chromatographic separation was achieved on a Spheri-5 RP-C8 (5 μm) (220 x 4.6 mm i.d.) column using a mobile phase composed of acetonitrile and 0.05 M sodium acetate, pH 4.0 (30 : 70, v/v) at a flow rate of 1 mL/min. The UV detector was set at 270 nm and the quantitation of the analytes was based on the peak areas. The method was proven to be accurate and precise with linearity ranges of 0.1-50 and 0.4-50 …

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Hepatoprotective And Antioxidative Properties Of Chinese Herbal Medicine Xiao-Chai-Hu-Tang Formulated With Bupleurum Kaoi Liu On Carbon Tetrachloride-Induced Acute Hepatotoxicity In Rats, C.-Y. Wu, Y.-M. Weng, C.-T. Liu, P.-T. Chuang, S.-Y. Liu, C.-Y. Tseng Jul 2020

Hepatoprotective And Antioxidative Properties Of Chinese Herbal Medicine Xiao-Chai-Hu-Tang Formulated With Bupleurum Kaoi Liu On Carbon Tetrachloride-Induced Acute Hepatotoxicity In Rats, C.-Y. Wu, Y.-M. Weng, C.-T. Liu, P.-T. Chuang, S.-Y. Liu, C.-Y. Tseng

Journal of Food and Drug Analysis

The Chinese herbal medicine, Xiao-Chai-Hu-Tang (XCHT), with Bupleurum radix as the main ingredient, is used for treating hepatitis. Although there are many varieties of Bupleurum in the herbal market, Bupleurum kaoi is the only indigenous species belonging to the Bupleurum genus plant in Taiwan which is commercially cultivated for making health-promoting products. The present study examined the hepatoprotective and antioxidative properties of XCHT formulated with commercially cultivated B. kaoi. The activities of aspartate aminotransferase (AST), alanine aminotransferase (ALT), superoxide dismutase (SOD), catalase, glutathione peroxidase (GPx), glutathione reductase (GRd) and glutathione-S-transferase (GST) as well as lipid peroxidation in rats treated with …

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Water-Soluble Phenolic Compounds And Their Anti-Hiv-1 Activities From The Leaves Of Cyclocarya Paliurus, J. Zhang, N. Huang, J.-C. Lu, X. Li, Y.-H. Wang, L.-M. Yang, Y.-T. Zheng, K. Xiao Jul 2020

Water-Soluble Phenolic Compounds And Their Anti-Hiv-1 Activities From The Leaves Of Cyclocarya Paliurus, J. Zhang, N. Huang, J.-C. Lu, X. Li, Y.-H. Wang, L.-M. Yang, Y.-T. Zheng, K. Xiao

Journal of Food and Drug Analysis

Eight phenolic compounds including five glucuronides were isolated from the leaves of Cyclocarya paliurus (Batal.) Ijinskaja, which is used as a source of tea. Their structures were characterized by spectroscopic methods. The results of biological assay showed that compounds 1 and 5 had good activities against HIV-1 induced cytopathic effects in C8166 lymphocyte at non-cytotoxic concentrations, with EC50 values of 31.74 and 10.76 μM, and therapeutic index (TI) values of >13.02 and 25.46, respectively. Relationship between molecular structures and their bioactivities was discussed.

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Dibenzoylmethane Altered Adipose Mass, Serum Lipid And Sex Steroid Hormone In Female Mice, C.-C. Lin, M.-T. Huang, C.-T. Ho Jul 2020

Dibenzoylmethane Altered Adipose Mass, Serum Lipid And Sex Steroid Hormone In Female Mice, C.-C. Lin, M.-T. Huang, C.-T. Ho

Journal of Food and Drug Analysis

Dibenzoylmethane (DBM), a β-diketone structural analogue of curcumin, has been reported to exhibit anti-tumorigenic and chemopreventive activities in mouse mammary tumorigenesis. Our previous study indicated that DBM modulates estrogenic action both in vitro and in vivo. In this report, the potential role of DBM as modulator of lipid metabolism in two mouse models was investigated. Several biomarkers and intermediate metabolites related to lipid consumption were measured. The results indicated that the parametrial fat pad weight, the level of triglyceride in both CD-1 and Senear mice fed with 1% DBM, was remarkably reduced. In addition, the estradiol level, uterus and mammary …

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Validated Stability-Indicating Thin Layer Chromatographic Determination Of Nadifloxacin In Microemulsion And Bulk Drug Formulations, A. Kumar, S. Sinha, S.P. Agarwal, J. Ali, A. Ahuja, S. Baboota Jul 2020

Validated Stability-Indicating Thin Layer Chromatographic Determination Of Nadifloxacin In Microemulsion And Bulk Drug Formulations, A. Kumar, S. Sinha, S.P. Agarwal, J. Ali, A. Ahuja, S. Baboota

Journal of Food and Drug Analysis

A stability-indicating high performance thin layer chromatographic (HPTLC) method for the densitometric analysis of nadifloxacin in microemulsions was developed and validated according to the guidelines of the International Conference on Harmonization (ICH). The compact spot for nadifloxacin was found at an Rf value of 0.39±0.02 at an absorption wavelength of 288 nm using a mobile phase consisting of chloroform : methanol : formic acid (7.5 : 2.0 : 0.5 v/v). The linear regression data for the calibration plots (r 2= 0.9981) was found with respect to peak area in the concentration range of 50-600 ng/spot. The limit of detection (LOD) …

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An Hplc Method For The Simultaneous Determination Of Marker Compounds Of Aloe And Scutellariae Radix In Cosmetics, K.-C. Wen, S.-L. Chan, L. Liu, P.-Y. Lai, Y.-T. Lin, S.-L. Hsiu, H.-M. Chiang Jul 2020

An Hplc Method For The Simultaneous Determination Of Marker Compounds Of Aloe And Scutellariae Radix In Cosmetics, K.-C. Wen, S.-L. Chan, L. Liu, P.-Y. Lai, Y.-T. Lin, S.-L. Hsiu, H.-M. Chiang

Journal of Food and Drug Analysis

The Department of Health in Taiwan has announced mandatory labeling of the whole composition of cosmetics since May 5, 2002. Chinese herbal extracts are commonly added to cosmetics to attract consumers. To follow the earlier-mentioned regulation, it is important to develop a quantitative method to examine the contents of products to which Chinese herbal extracts have been added. This study attempted to establish a quantitative method for aloe and Scutellariae Radix (SR) in cosmetics. An HPLC/UV method using gradient elution was established to analyze marker compounds in herbal extracts and spiked blank cosmetic bases. The calibration curves of the marker …

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Antioxidant Activity Of Aqueous Extract Fractions Of Velvet Antler (Census Elaphus Linnaeus), L. Zhao, R.-S. Pei, B.-P. Ji, Y.-C. Luo, D. Zhang, Z.-Y. Xu, X.-N. Jia Jul 2020

Antioxidant Activity Of Aqueous Extract Fractions Of Velvet Antler (Census Elaphus Linnaeus), L. Zhao, R.-S. Pei, B.-P. Ji, Y.-C. Luo, D. Zhang, Z.-Y. Xu, X.-N. Jia

Journal of Food and Drug Analysis

Velvet antler is believed to have body strengthening, immunomodulatory and anti-aging effects. It is used in Chinese commercial functional foods and nutraceuticals. The antioxidant activity of the aqueous extract of velvet antler (AEVA) from Cervus elaphus Linnaeus was evaluated with DPPH-radical scavenging, FRAP, Fe2+-chelating and inhibition of linoleic acid autoxidation assays. AEVA showed antioxidant activity in all four assays. After removal of protein from AEVA, the antioxidant activity was significantly elevated. A semi-preparative HPLC equipped with a C18 column was used for further separation of the non-protein components (AEVA-S). Identification of the most active fraction (AEVA-SII) of AEVA-S was accomplished …

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Carbon Surfaces For The Oxidative Quantification Of Pravastatin: Glassy-Carbon Vs. Screen-Printed Carbon Electrodes, M.M.P.S. Neves, H.P.A. Nouws, C. Delerue-Matos Jul 2020

Carbon Surfaces For The Oxidative Quantification Of Pravastatin: Glassy-Carbon Vs. Screen-Printed Carbon Electrodes, M.M.P.S. Neves, H.P.A. Nouws, C. Delerue-Matos

Journal of Food and Drug Analysis

The electrooxidative behavior of pravastatin (PRV) in aqueous media was studied by square-wave voltammetry at a glassy-carbon electrode (GCE) and at a screen-printed carbon electrode (SPCE). Maximum peak current intensities in a pH 5.0 buffer were obtained at +1.3 V vs. AgCl/Ag and +1.0 V vs. Ag for the GCE and SPCE surface respectively. Validation of the developed methodologies revealed good performance characteristics and confirmed their applicability to the quantification of PRV in pharmaceutical products, without significant sample pretreatment. A comparative analysis between the two electrode types showed that SPCEs are preferred as an electrode surface because of their higher …

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Flavonoids From Taxillus Theifer, T.-H. Tsai, Y.-C. Liu, L.-C. Lin Jul 2020

Flavonoids From Taxillus Theifer, T.-H. Tsai, Y.-C. Liu, L.-C. Lin

Journal of Food and Drug Analysis

A new catechin derivative, catechin-5-O-(6-O-galloyl-β- glucopyranoside) (2), together with ten known flavonoids, was isolated from the leaves and stems of Taxillus theifer (Hayata) H. S. Kiu. T. theifer is a shrub parasitizing on different species of plants. Three samples of T. theifer on various host plants were collected and their chemical constituent variations were identified and estimated by HPLC/UV methods. A comparison of the constituents from T. theifer parasitizing on different host trees demonstrated that catechin (1), quercetin-3-O-(6-O-galloyl-β-glucopyranoside) (3), quercetin-3-O-β-glucopyranoside (5), and quercetin-3-O-β-glucuronide (6) were the major components in the title plant irrespective of the host tree. However, the content …

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Spectrophotometric Determination Of Chloroquine, Pyrimethamine And Trimethoprim By Ion Pair Extraction In Pharmaceutical Formulation And Urine, P. Nagaraja, A.K. Shrestha, A. Shivakumar, A.K. Gowda Jul 2020

Spectrophotometric Determination Of Chloroquine, Pyrimethamine And Trimethoprim By Ion Pair Extraction In Pharmaceutical Formulation And Urine, P. Nagaraja, A.K. Shrestha, A. Shivakumar, A.K. Gowda

Journal of Food and Drug Analysis

A simple and accurate method for the determination of chloroquine diphosphate (CQ), pyrimethamine (PYM) and trimethoprim (TMP) in pure, pharmaceutical and urine samples is described. The method involved an ion pair extraction procedure with chloroform using an acid base indicator, bromocresol purple (BCP), as a counter ion. The ion pair extracted in organic phase was yellow, and the absorbance was measured at λmax = 420 nm. Beer's law was obeyed over the concentration range of 1.25-8.75, 0.62-7.5, and 1.25-10.71 μg/mL, respectively. The Sandell's sensitivity was 0.01258, 0.01102 and 0.01464 μg/cm2. The Ringbom optimum concentration range, which was 2.5-7.5, 1.25-7.5 and …

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Species Identification Of A Causative Moray Eel Meat By Sds-Page, Y.-C. Huang, T.-Y. Chen, S.-S. Jeng, H.-M. Chen, D.-F. Hwang Jul 2020

Species Identification Of A Causative Moray Eel Meat By Sds-Page, Y.-C. Huang, T.-Y. Chen, S.-S. Jeng, H.-M. Chen, D.-F. Hwang

Journal of Food and Drug Analysis

Attempts were made to identify the species of food poisoning moray eel. Besides the causative processed moray eel meat, eight other raw commercial moray eel meats including Gymnothorax favagineus, G.fimbriatus, G. flavimarginatus, G. meleagris, G. pseudothyrsoideus, G. undulates, G. albimarginatus and G. javanicus were heated at 100°C for 30 min and then assayed using the electrophoresis method of sodium dodecyl sulfate Polyacrylamide gel electrophoresis (SDS-PAGE). The SDS-PAGE patterns for eight processed commercial moray eel meats showed species-specific protein bands < 30 kD. The species of causative processed moray eel implicated in food poisoning case was identified as G. javanicus in comparison to the 2% SDS and 8 M urea extracted protein revealed by SDS-PAGE patterns.

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Genetic Identification Of Cinnamomum Species Based On Partial Internal Transcribed Spacer 2 Of Ribosomal Dna, S.-C. Lee, C.-H. Lee, M.-Y. Lin, K.-Y. Ho Jul 2020

Genetic Identification Of Cinnamomum Species Based On Partial Internal Transcribed Spacer 2 Of Ribosomal Dna, S.-C. Lee, C.-H. Lee, M.-Y. Lin, K.-Y. Ho

Journal of Food and Drug Analysis

The present study investigates the genetic identification of Cinnamomum species by analyzing the nucleotide sequences of the partial non-coding internal transcribed spacer 2 (ITS2) of ribosomal genes. The genetic diversity of the five Cinnamomum species (Cinnamomum cassia, C. zeylanicum, C. loureiroi, C. osmophloeum and C. burmannii) was studied using polymerase chain reaction (PCR) amplification and a DNA sequencing method to molecularly identify the tested specimens. Phylogenetic trees were constructed using several clustering methods, including unweighted pair-group method with arithmetic average (UPGMA), neighbor-joining (NJ) and maximum evolution (ME). The eleven test samples were clustered into three major groups based on partial …

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Analysis Of Soy Isoflavones In Foods And Biological Fluids: An Overview, B.-Y. Hsu, B.S. Inbaraj, B.-H. Chen Jul 2020

Analysis Of Soy Isoflavones In Foods And Biological Fluids: An Overview, B.-Y. Hsu, B.S. Inbaraj, B.-H. Chen

Journal of Food and Drug Analysis

Soy isoflavones are phytoestrogens mainly present in soybean and soybean products. Isoflavones have been reported to possess physiological activities such as inhibition of cancer cell proliferation, reduction of cardiovascular risk, prevention of osteoporosis and alleviation of postmenopausal syndrome. This paper gives an overview of soy isoflavone analysis in foods and biological fluids. Extraction of isoflavones is often carried out by polar solvents such as methanol, ethanol, acetone or acetonitrile, or in combination with acid-containing water. The separation, identification and quantification of isoflavones are usually conducted by gas chromatography coupled with flame ionization detector or mass spectrometer (GC/FID or GC/MS), high-performance …

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Foam Properties, Detergent Abilities And Long-Term Preservative Efficacy Of The Saponins From Sapindus Mukorossi, C.-H. Yang, Y.-C. Huang, Y.-F. Chen, M.-H. Chang Jul 2020

Foam Properties, Detergent Abilities And Long-Term Preservative Efficacy Of The Saponins From Sapindus Mukorossi, C.-H. Yang, Y.-C. Huang, Y.-F. Chen, M.-H. Chang

Journal of Food and Drug Analysis

Pericarp of Sapindus mukorossi Gaertn has been used as a natural detergent and its extract is commercially utilized as a foam-stabilizing and emulsifying agent. The goal of this study is to investigate the foam properties, detergent ability and long-term preservative efficacy of the saponins from the pericarp of S. mukorossi Gaertn. The crude saponin content in the pericarp of S. mukorossi Gaertn was 14.2% and the total saponins content in the crude saponins extract was 85% (w/w). The foaming power of the 0.5% crude saponins extract solution from 5. mukorossi Gaertn is 65% of 0.5% SLS solution and similar to …

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Dietary Cholestin (Red Yeast Extract) Reduces Toxicity Of Oxidized Cholesterol In Rats, Y.-H. Yeh, Y.-T. Lee, H.-S. Hsieh, D.-F. Hwang Jul 2020

Dietary Cholestin (Red Yeast Extract) Reduces Toxicity Of Oxidized Cholesterol In Rats, Y.-H. Yeh, Y.-T. Lee, H.-S. Hsieh, D.-F. Hwang

Journal of Food and Drug Analysis

This study focused on the effects of dietary cholestin (red yeast extract) on the toxicity of oxidized cholesterol in rats. The rats were divided into eight groups and fed with or without supplement of 1% cholestin and 3% oxidized cholesterol in their diets for 8 weeks. It was found that cholestin could improve the body weight decrease and the glutathione (GSH) level in the liver, and increase of liver and kidney weight to body weight ratio, and thiobarbituric acid-reactive substances (TBARS) level in the liver of rats caused by oxidized cholesterol (P < 0.05). It also reduced the plasma content of alanine transaminase (ALT), aspartate transaminase (AST) and alkaline phosphatase (ALP), indicating that cholestin could prevent the hepatotoxicity induced by oxidized cholesterol (P < 0.05). In addition, cholestin possessed recovering effect and a short-term preventing effect on the toxicity of oxidized cholesterol in rats. Taking all these data together, cholestin may play an important role in diminishing the toxic effects of oxidized cholesterol in rats.

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Anti-Inflammatory Activity Of Soymilk And Fermented Soymilk Prepared With Lactic Acid Bacterium And Bifidobacterium, C.-L. Liao, H.-Y. Huang, L.-Y. Sheen, C.-C. Chou Jul 2020

Anti-Inflammatory Activity Of Soymilk And Fermented Soymilk Prepared With Lactic Acid Bacterium And Bifidobacterium, C.-L. Liao, H.-Y. Huang, L.-Y. Sheen, C.-C. Chou

Journal of Food and Drug Analysis

In the present study, anti-inflammatory activity of the methanol extracts of soymilk and soymilk fermented with Streptococcus salivarius subsp. thermophilus CCRC 14805 alone or in conjunction with Bificobacterium infantis CCRC 14603 was evaluated. It was found that extracts of soymilk and the prepared fermented soymilk dose-dependently suppress nitric oxide (NO) production of lipopolysaccharide (LPS)-induced macrophages, with soymilk showing the highest inhibition ability followed by S. thermophilus-fermented soymilk. Extracts of soymilk and fermented soymilk did not affect the activity of inducible nitric oxide synthase (iNOS). The extracts of the fermented soymilk inhibited the protein expression of iNOS in LPS-induced macrophages, but …

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Molecular Authentication Of The Chinese Herb Huajuhong And Related Medicinal Material By Dna Sequencing And Issr Markers, C. Su, K.-L. Wong, P.P.-H. But, W.-W. Su, P.-C. Shaw Jul 2020

Molecular Authentication Of The Chinese Herb Huajuhong And Related Medicinal Material By Dna Sequencing And Issr Markers, C. Su, K.-L. Wong, P.P.-H. But, W.-W. Su, P.-C. Shaw

Journal of Food and Drug Analysis

DNA sequences in the chloroplast (trnH-psbA intergenic spacer) and nuclear (ITS) regions were amplified and determined for Huajuhong derived from the peels of the immature fruits of Citrus grandis 'Tomentosa' and related medicinal material. These sequences together with the inter-simple sequence repeats (ISSR) markers may be used to differentiate C. grandis 'Tomentosa' from other Citrus variants for the prevention of misuse.

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Chemical Fingerprint Analysis Of Gardenia Jasminoides Fruit By High-Performance Liquid Chromatography, Y. Ding, L.-Y. Xiang, T. Zhang, S. Ji, L.-Y. Xu Jul 2020

Chemical Fingerprint Analysis Of Gardenia Jasminoides Fruit By High-Performance Liquid Chromatography, Y. Ding, L.-Y. Xiang, T. Zhang, S. Ji, L.-Y. Xu

Journal of Food and Drug Analysis

A high performance liquid chromatography-ultraviolet detector-electrospray ionization tandem mass spectrometry (HPLC/UV/ESI/MSn) method was used for the chromatographic fingerprint analysis of Gardenia jasminoides fruit, Fructus Gardeniae (zhi-zi in Chinese). Thirteen batches of samples collected from 8 provinces were analyzed to standardize the fingerprint of zhi-zi. The thirteen peaks in the fingerprints of all the 13 batches of samples with reasonable height and good resolution were assigned as "characteristic peaks". Seven "characteristic peaks" were identified by comparing their retention time and MS (mass spectrum) with those of the reference substances for the first time. The Similarity Evaluation System for Chromatographic Fingerprint of …

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Antioxidant And Modulatory Effect Of Ethanolic Extract Of Madagascar Harungana (Harungana Madagascariensis) Bark On Cyclophosphamide Induced Neurotoxicity In Rats, G. Oboh, T.L. Akomolafe, S.A. Adefegha, A.O. Adetuyi Jul 2020

Antioxidant And Modulatory Effect Of Ethanolic Extract Of Madagascar Harungana (Harungana Madagascariensis) Bark On Cyclophosphamide Induced Neurotoxicity In Rats, G. Oboh, T.L. Akomolafe, S.A. Adefegha, A.O. Adetuyi

Journal of Food and Drug Analysis

The present study focused on the antioxidant and modulatory effects of the ethanolic extract of Madagascar Harungana bark on cyclophosphamide induced oxidative stress in rat's brain. Ethanolic extract of Madagascar Harungana bark yielded a yellow dye. Subsequently, the phytochemical screening and antioxidant activities, including total phenol content, free radical scavenging ability, reducing power, Fe2+ chelating ability and in vitro inhibition of lipid peroxidation in rat's brain, were determined. The protective effect of dietary inclusion of these extracts (0.5 and 1.0%) on cyclophosphamide (75 mg/kg body weight) induced oxidative stress in rat's brain was also assessed. The dietary supplementation of Madagascar …

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In Vitro Effects Of Lactic Acid Bacteria On Cancer Cell Viability And Antioxidant Activity, C.-F. Liu, T.-M. Pan Jul 2020

In Vitro Effects Of Lactic Acid Bacteria On Cancer Cell Viability And Antioxidant Activity, C.-F. Liu, T.-M. Pan

Journal of Food and Drug Analysis

We used two local Lactobacillus strains (L. paracasei ssp. paracasei NTU 101 and L. plantarum NTU 102) and other 10 lactic acid bacteria (LAB) strains from the Biosource Collection and Research Center (BCRC) to evaluate the health promoting capability including inhibitory effects on cancer cell lines and antioxidant activities in vitro. The heat-killed cells (HKC) of Lactobacillus acidophilus BCRC14079 have the highest total antioxidative activity (82.3%). 1,1-diphenyl-2-picryl-hydrazyl (DPPH) radical scavenging effects were in the range of 14.8-32.9%, except for the HKC of L. salivarius ssp. salivarius BCRC 14759 and Bifidobacterium breve BCRC 11846. Twelve strains showed varying degrees of reducing …

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Dna Barcoding Cinnamomum Osmophloeum Kaneh. Based On The Partial Non-Coding Its2 Region Of Ribosomal Genes, S.-C. Lee, S.-J. Chiou, J.-H. Yen, T.-Y. Lin, K.-T. Hsieh, J.-C. Yang Jul 2020

Dna Barcoding Cinnamomum Osmophloeum Kaneh. Based On The Partial Non-Coding Its2 Region Of Ribosomal Genes, S.-C. Lee, S.-J. Chiou, J.-H. Yen, T.-Y. Lin, K.-T. Hsieh, J.-C. Yang

Journal of Food and Drug Analysis

This paper investigated the DNA barcoding of indigenous cinnamon. The essential oil composition of leaves and the partial non-coding internal transcribed spacer (ITS2) region of ribosomal genes from indigenous cinnamon plants (Cinnamomum osmophloeum Kaneh.) were investigated. Leaf essential oils from seven geographical strains of C. osmophloeum Kaneh. were obtained by hydrodistillation and characterized by gas chromatography-mass spectrometry. Six chemotypes, including cinnamaldehyde, cinnamaldehyde/cinnamyl acetate, cinnamyl acetate, linalool, camphor and mixed, were identified. Seven representative geographical strains were further used to study their genetic diversity based on the partial ITS2 region using PCR amplification and DNA sequencing to evaluate the region's use …

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Tortoise Dna Detection From Highly Processed Tortoise Shell Using Sine Element, P. Lv, X. Zhou, J. You, Y. Qin, B.-C. Ye, Y. Zhang Jul 2020

Tortoise Dna Detection From Highly Processed Tortoise Shell Using Sine Element, P. Lv, X. Zhou, J. You, Y. Qin, B.-C. Ye, Y. Zhang

Journal of Food and Drug Analysis

Colla carapax et plastrum testudinis is one of the most valuable traditional Chinese medicine referring to highly processed tortoise shell glue which contains trace amount of severely degraded DNA. In this work, a simple and fast methodology was first developed for the identification of highly processed medicine from substitutions which were labeled fraudulently as tortoise shell glue but derived from tissues of other animals such as horse, cattle or pig. The method is based on polymerase chain reaction (PCR) amplification of tortoise-specific Pol III/SINE element and allows the detection of tortoise DNA in glue mixtures, which contain only 0.1% colla …

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Purification And Characterization Of A Novel Fibrinolytic Protease From Schizophyllum Commune, C.-L. Lu, S. Chen, S.-N. Chen Jul 2020

Purification And Characterization Of A Novel Fibrinolytic Protease From Schizophyllum Commune, C.-L. Lu, S. Chen, S.-N. Chen

Journal of Food and Drug Analysis

Schizophyllum commune, a widely distributed medicinal mushroom, was found with fibrinolytic activity from its basidiomycetes. The fibrinolytic activity of S. commune was to be beneficial for antithrombotic therapy. In this study, S. commune was cultured with fermentation technology, and protease purification was carried out by cross-flow filtration and fast performance liquid chromatography (FPLC) system. The specific activity of S. commune fibrinolytic protease increased 9.29-fold over the culture broth after purification. The fibrinolytic protease shows superior fibrinolytic activity than human plasmin and is inhibited by EDTA. Characterizations of this protease showed 21.32 kDa in molecular mass and monomeric form in protein …

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Simultaneous Determination Of 18 Quinolone Residues In Marine And Livestock Products By Liquid Chromatography/Tandem Mass Spectrometry, C.-S. Chang, W.-H. Wang, C.-E. Tsai Jul 2020

Simultaneous Determination Of 18 Quinolone Residues In Marine And Livestock Products By Liquid Chromatography/Tandem Mass Spectrometry, C.-S. Chang, W.-H. Wang, C.-E. Tsai

Journal of Food and Drug Analysis

A simple and efficient LC/ESI/MS/MS method was developed to determine 18 (fluoro)quinolone (QNs) residues in milk, chicken, pork, fish and shrimp. This method is capable of screening and confirming the presence of 12 amphoteric QNs (marbofloxacin, norfloxacin, enrofloxacin, ciprofloxacin, desethylene ciprofloxacin, lomefloxacin, danofloxacin, sarfloxacin, difloxacin, ofloxacin, orbifloxacin and enoxacin) and 6 acidic QNs (oxolinic acid, nalidixic acid, flumequine, cinoxacin, piromidic acid and pipemidic acid). The drugs were extracted from matrix with acetonitrile containing 1% formic acid, diluted in 10% acetonitrile and defatted by extraction with hexane. The LC separation was conducted on an XDB C-8 (150 mm x 4.6 mm, …

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Quantitative Determination Of Four Nitrofurans And Corresponding Metabolites In The Fish Muscle By Liquid Chromatography-Electrospray Ionization-Tandem Mass Spectrometry, C.-W. Tsai, C.-H. Tang, W.-H. Wang Jul 2020

Quantitative Determination Of Four Nitrofurans And Corresponding Metabolites In The Fish Muscle By Liquid Chromatography-Electrospray Ionization-Tandem Mass Spectrometry, C.-W. Tsai, C.-H. Tang, W.-H. Wang

Journal of Food and Drug Analysis

A method validated based on the European Union (EU) regulations for determining the presence of furazolidone, furaltadone, nitrofurazone, nitrofurantoin and their corresponding metabolites AOZ, AMOZ, SC and AH in fish muscle was developed. Samples were acid-hydrolyzed, treated with 2-nitrobenzaldehyde and extracted with ethyl acetate. The extracts were dried in a N2 stream and redissolved in methanohwater (50:50, v/v). Analysis was performed by LC/ESI/MS/MS. This developed method carried limits of quantification lower than 10 μg/kg for the nitrofurans and 1.0 μg/kg for the metabolites. It was observed that the decision limit (CCα) ranged from 2.93 to 5.01 μg/kg for the nitrofurans …

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Determination Of 17Α-Methyltestosterone In Bovine Serum Using Liquid Chromatography Tandem Mass Spectrometry, R. Patricia, N. Carolina, B.I. Vázquez, C. Alberto, C.A. Fente Jul 2020

Determination Of 17Α-Methyltestosterone In Bovine Serum Using Liquid Chromatography Tandem Mass Spectrometry, R. Patricia, N. Carolina, B.I. Vázquez, C. Alberto, C.A. Fente

Journal of Food and Drug Analysis

A high performance liquid chromatography tandem mass spectrometry (HPLC-MS/MS) based method has been developed and validated to confirm the presence of the synthetic growth promoter 17α-methyltestosterone (MT) in bovine serum samples. After a single-step extraction with acetonitrile, the enhanced determination of methyltestosterone was achieved by detecting its oxime derivative. In accordance with the criteria defined in Commission Decision 2002/657/EC and using deuterated testosterone (T-d3) as an internal standard, the method has been validated in terms of decision limit (0.05 ng/mL), detection capability (0.09 ng/mL), repeatability CV (7.04%), within-laboratory reproducibility CV (11.00%), and accuracy (87.00%).

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Identification Of Ginseng Radix In Chinese Medicine Preparations By Nested Pcr-Dna Sequencing Method And Nested Pcr-Restriction Fragment Length Polymorphism, K.T. Lu, H.C. Lee, F.S. Liu, C.F. Lo, J.H. Lin Jul 2020

Identification Of Ginseng Radix In Chinese Medicine Preparations By Nested Pcr-Dna Sequencing Method And Nested Pcr-Restriction Fragment Length Polymorphism, K.T. Lu, H.C. Lee, F.S. Liu, C.F. Lo, J.H. Lin

Journal of Food and Drug Analysis

Ginseng Radix, the dried root of Panax ginseng, is easily confused with other herbs of the Panax species by appearance. In Chinese medicine preparations, it is difficult to identify misuse of Ginseng Radix. In this study, the nested PCR and DNA sequencing methods were established for the identification of Ginseng Radix, whereas the combination of nested PCR and restriction fragment length polymorphism (RFLP) could differentiate Ginseng Radix from other Panax species and thus certify the use of impure raw material of Ginseng Radix in Chinese medicine preparations. Nested PCR and DNA sequencing methods were applied to verify ginseng ingredients in …

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Aflatoxin In Raw Peanut Kernels Marketed In Malaysia, S. Arzandeh, J. Selamat, H. Lioe Jul 2020

Aflatoxin In Raw Peanut Kernels Marketed In Malaysia, S. Arzandeh, J. Selamat, H. Lioe

Journal of Food and Drug Analysis

The occurrence of aflatoxin in eighty-four samples of raw peanut kernels which are randomly collected from Malaysian supermarkets was examined. Analysis for aflatoxin was performed by solvent extraction and immunoaffinity clean-up followed by the determination using high performance liquid chromatography equipped with post-column photochemical reactor for enhanced detection and fluorescence detector. A detection limit of 0.01-0.09 ng/mL and a quantification limit of 0.04-0.30 ng/mL were obtained. The aflatoxin concentrations ranged from not detected to 97.28 ng/g in all samples investigated. About 78.57% of the samples were contaminated with aflatoxin, of which 10.71% exceeded the maximum tolerable limit of 15 ng/g …

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Date Fiber Concentrate: Chemical Compositions, Functional Properties And Effect On Quality Characteristics Of Beef Burgers, S. Besbes, R. Ghorbel, R.B. Salah, M. Masmoudi, F. Jedidi, H. Attia, C. Blecker Jul 2020

Date Fiber Concentrate: Chemical Compositions, Functional Properties And Effect On Quality Characteristics Of Beef Burgers, S. Besbes, R. Ghorbel, R.B. Salah, M. Masmoudi, F. Jedidi, H. Attia, C. Blecker

Journal of Food and Drug Analysis

Chemical composition of second-grade dates (with hard texture) from Tunisian Deglet Nour cultivar was similar to that of commercial dates. Date fiber concentrate (DFC) was extracted and characterized in terms of chemical composition and techno-functional properties. DFC showed interesting functional properties. In fact, it presented high water binding capacities (WBC) and oil binding capacities (OBC) reaching 15.82 g/g and 11.31 g/g, respectively. These values were higher than those reported for the most fruits and vegetable fiber concentrates. The use of DFC in beef burger formulations improves cooking properties, e.g. increase cooking yield and decrease shrinkage and minimize production cost without …

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Proteinaceous Constituents Of Red Cabbage Juice Increase Il-10, But Decrease Tnf-Α Secretions Using Lps-Stimulated Mouse Splenocytes, J.-Y. Lin, C.-Y. Li Jul 2020

Proteinaceous Constituents Of Red Cabbage Juice Increase Il-10, But Decrease Tnf-Α Secretions Using Lps-Stimulated Mouse Splenocytes, J.-Y. Lin, C.-Y. Li

Journal of Food and Drug Analysis

This study investigates the anti-inflammatory effects of proteinaceous constituents from red cabbage (Brassica oleracea L. var.) juice (RC). The RC was first absorbed using activated charcoal (RCA), then partially purified using a Sephadex LH-20 column and finally precipitated using saturated ammonium sulfate solution to obtain the protein-rich fractions. The crude and protein-rich samples from RC were administered to lipopolysaccharide (LPS) - stimulated BALB/c mouse splenocyte cultures for 48 hours. The results showed that the lyophilized RC powder was rich in protein (30.2 ± 0.2%), carbohydrate (33.8 ± 6.0%), phenolics (2.8 ± 0.3%), and flavonoids (1.1 ± 0.1%). After the activated …

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