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2020

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Simultaneous Determination Of Bromhexine Hcl And Baicalin In Chinese Compound Medicine By A Reversed-Phase Ion-Pair Hplc, S. Liu, L. Zhao, X. Liu, F. Zhou, S. Jiang Jul 2020

Simultaneous Determination Of Bromhexine Hcl And Baicalin In Chinese Compound Medicine By A Reversed-Phase Ion-Pair Hplc, S. Liu, L. Zhao, X. Liu, F. Zhou, S. Jiang

Journal of Food and Drug Analysis

In this article, a reversed-phase ion-pair high-performance liquid chromatography (HPLC) method was established for simultaneous separation and quantitative analysis of bromhexine HCl (BHH) and baicalin in Chinese Ke-Chuan-Ling compound capsule. The influence of the mobile phase composition on the retention of the analytes was investigated. The optimal determination conditions consisted of acetonitrile/water/phosphoric acid/triethylamine (22/78/0.1/0.1, v/v/v/v) mobile phase with the addition of tetra-n-butylammonium bromide (TBA) (0.7 mmol L-1, terminal concentration) as pairing ion, and a detection wavelength at 225 nm. In this case, baicalin and bromhexine HCl were eluted at 21 min and 27 min respectively and were separated successfully from …

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Study On Isoflavones Isomers Contents In Taiwan's Soybean And Gm Soybean, Q.-K. Wei, W.W. Jone, T.J. Fang Jul 2020

Study On Isoflavones Isomers Contents In Taiwan's Soybean And Gm Soybean, Q.-K. Wei, W.W. Jone, T.J. Fang

Journal of Food and Drug Analysis

In this report we compared the total contents and the distribution of isoflavones in six soybeans, including 1 imported genetically modified (GM) soybean and 5 Taiwanese nongenetic modified soybeans, Koushing 10, Tainan 1, Tainan 4, Tainan 5 black, and commercial black. After the soybeans were soaked for 0, 4, 8, 12 and 16 hr in the solutions with different pH values (pH 3, 7 and 9), the contents of the isoflavones in the different 6 soybeans were measured. Nine isoflavones, including daidzin, genistin, and glycitin with a glucoside; acetyl-daidzin, acetyl-genistin, and acetyl-glycitin with acetylglucoside; and daidzein, genistein, and glycitein aglycones, …

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Establishment Of Quantitative Method For Five Events Of Genetically Modified Maize (Bt11, Event176, Ga21, Mon810 And T25) By Real-Time Qpcr, L.-C. Chiueh, C.-Y. Lin, Y.-C. Chen, M.-H. Wu, D.Y.-C. Shih Jul 2020

Establishment Of Quantitative Method For Five Events Of Genetically Modified Maize (Bt11, Event176, Ga21, Mon810 And T25) By Real-Time Qpcr, L.-C. Chiueh, C.-Y. Lin, Y.-C. Chen, M.-H. Wu, D.Y.-C. Shih

Journal of Food and Drug Analysis

In order to meet the implementated labeling requirement, this study focused on the development of real-time quantitative polymerase chain reaction (real-time QPCR) method to detect genetically modified maize (GM-maize). Primers and probes specific for inserted genes in Event176, Bt11 (Syngenta company), MON810, GA21 (Monsanto company) and T25 GM-maize (Aventis company) were designed and used to conduct the real-time QPCR assays. A plasmid containing both transgene and internal control gene targets on the same molecules for each GM-maize event was also constructed as quantitative reference molecules. Further, constructed plasmids and test samples were applied to validate this quantitative system. Results showed …

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Comparison Between Headspace And Aqueous Phase Solid Phase Microextraction Method For Speciation Analysis Of Butyltin Compounds In Sea Water, M.-P. Hsia, T.-B. Hsu, S.-M. Liu Jul 2020

Comparison Between Headspace And Aqueous Phase Solid Phase Microextraction Method For Speciation Analysis Of Butyltin Compounds In Sea Water, M.-P. Hsia, T.-B. Hsu, S.-M. Liu

Journal of Food and Drug Analysis

Headspace and aqueous phase solid phase microextraction (SPME) mehtods for the analysis of butyltin compounds in sea water were evaluated and compared. The method detection limit (MDL) for monobutyl- (MBT), dibutyl-(DBT), and tributyltin (TBT) in sea water were detected as 3.5, 3.8, and 3.8 ng/L, respectively, by the headspace SPME method. On the other hand, the MDL for MBT, DBT, and TBT in sea water were detected as 19.2, 20.4, and 31.7 ng/L, respectively, by the aqueous phase SPME method. Butyltin compounds in seawater collected from different harbors in Taiwan had MBT in the range of 5.5-51.9, DBT

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Stability Of Total Ascorbic Acid In Dtt-Preserved Plasma Stored At 4° C, S.-C. Lee, S.-M. Tsai, S.-K. Lin, B.-H. Chen, L.-Y. Tsai Jul 2020

Stability Of Total Ascorbic Acid In Dtt-Preserved Plasma Stored At 4° C, S.-C. Lee, S.-M. Tsai, S.-K. Lin, B.-H. Chen, L.-Y. Tsai

Journal of Food and Drug Analysis

In order to evaluate the stability of total ascorbic acid (TAA) after storage in refrigeration at 4°C, EDTA-blood was collected from 12 volunteers and preserved with dithiothreitol (DTT) immediately. After sampling, the blood was portioned into 19 micro-centrifuge tubes. One tube of sample was examined immediately by the capillary electrophoresis (CE) method, while the others were stored in refrigeration at 4°C until analysis. TAA analysis were performed once every 4 hr for a total of 72 hr after the sample collection. Our results showed that TAA concentrations in these specimens did not alter significantly within the first 16 hr of …

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Water Status Of Two Gelatin Gels During Storage As Determined By Magnetic Resonance Imaging, Y.-L. Chung, H.-M. Lai Jul 2020

Water Status Of Two Gelatin Gels During Storage As Determined By Magnetic Resonance Imaging, Y.-L. Chung, H.-M. Lai

Journal of Food and Drug Analysis

A two-gelatin-gel model system was used to investigate the water status of two gels with different Aw, 0.968 (H-gel) and 0.828 (L-gel), using magnetic resonance imaging (MRI) techniques. A multi slice-multi echo pulse sequence was applied and the transverse relaxation time (T2) of water protons was calculated. During storage the average T2 values of H-gel decreased, while those of L-gel increased. However, at the end of storage (15 days) when the Aw of two gels were the same, the average T2 values of L-gel were larger than those of H-gel, and heterogeneous T2 values were observed in both gels. This …

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Solid-Phase Extraction And Gc-Msd Determination Of Amitraz And Metabolites In Urine, C.-P. Chou, H.-P. Li, S.-S. Wong, G.-C. Li Jul 2020

Solid-Phase Extraction And Gc-Msd Determination Of Amitraz And Metabolites In Urine, C.-P. Chou, H.-P. Li, S.-S. Wong, G.-C. Li

Journal of Food and Drug Analysis

Urine, samples were collected from seven farmers before and after applying 20% amitraz EC to pear and citrus fields. Amitraz and its metabolites including BTS-27919, BTS-27271, and 2,4-dimethylaniline were eluted with n-hexane, dichloromethane and methanol in sequence from SPE-C18 cartridge by loading 5 mL of urine before GC-MSD determination. The mean recoveries of amitraz, BTS-27919, BTS-27271 and 2,4-dimethylaniline were 75.7 ± 4.2 , 81.3 ± 2.4 , 87.7 ± 3.7 and 83.3 ± 1.8%, respectively. Neither amitraz nor metabolites were detected in any of the seven farmers' urine samples.

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Extending The Cdna Microarray Detection System To Evaluate Genetically Modified Soybean And Traditional Soy Foods, T.-L. Chen, V. Prasad, C.-H. Lee, K.-H. Lin, L.-C. Chiueh, M.-T. Chan Jul 2020

Extending The Cdna Microarray Detection System To Evaluate Genetically Modified Soybean And Traditional Soy Foods, T.-L. Chen, V. Prasad, C.-H. Lee, K.-H. Lin, L.-C. Chiueh, M.-T. Chan

Journal of Food and Drug Analysis

Aggravating controversies of GM (genetically modified) foods on the social, ethical and health aspects have lead to efficient and reliable detection systems to safeguard homogeneity of foods and address legal disputes. Improvising on the existing detection systems, the present investigation demonstrates the advantages of using cDNA microarray as a detection system for GM food. We have extended the study for the detection of genetic modification in commercial GM soybean seeds and in home-made traditional foods derived from these seeds, such as tofu and dried tofu. We looked for common T-DNA regions such as CaMV35S promoter, NOS or 35S terminators, nptII, …

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Comparison Of Microwave, Dry And Wet Digestion Procedures For The Determination Of Trace Metal Contents In Spice Samples Produced In Turkey, M. Soylak, M. Tuzen, I. Narin, H. Sari Jul 2020

Comparison Of Microwave, Dry And Wet Digestion Procedures For The Determination Of Trace Metal Contents In Spice Samples Produced In Turkey, M. Soylak, M. Tuzen, I. Narin, H. Sari

Journal of Food and Drug Analysis

The concentrations of trace metals (Pb, Cd, Cr, Ni, Fe, Cu, Mn and Zn) in spice samples from Turkey were determined because similar studies have not been published. The experiments were carried out using atomic absorption spectrometry after dry ashing, wet ashing and microwave digestion methods. The study of sample preparation procedures showed that the microwave method was the best. The proposed method showed satisfactory recovery, detection limits and standard deviation for trace metal determination in spice samples. The concentrations of Pb, Cd, Cr, Ni, Fe, Cu, Mn and Zn in the samples were in the range of 0.47-1.89, 0.10-0.93, …

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Erratum: (Journal Of Food And Drug Analysis Vol. 12 (2) (140)), Skip Jul 2020

Erratum: (Journal Of Food And Drug Analysis Vol. 12 (2) (140)), Skip

Journal of Food and Drug Analysis

[No abstract available]

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Hplc/Colorimetry Combination Method For Quantitative Analyses Of Sclerotiorin Produced By Penicillium Sclerotiorum, S.-H. Weng, N.-W. Su, Y.-M. Choong, M.-H. Lee Jul 2020

Hplc/Colorimetry Combination Method For Quantitative Analyses Of Sclerotiorin Produced By Penicillium Sclerotiorum, S.-H. Weng, N.-W. Su, Y.-M. Choong, M.-H. Lee

Journal of Food and Drug Analysis

In this study, quantitative analysis of sclerotiorin from Penicillium sclerotiorum was established by using HPLC/colorimetry combination method at the condition of unavailability of authentic sclerotiorin compound. Mycelium was extracted with methanol, then analyzed by HPLC (a C18 reverse phase column with acetonitrile/water = 65/35, v/v as mobile phase and detected at 370 nm) and simultaneously measured OD370 with spectrophotometers. The limit of quantitation (LOQ) was 0.5 μg/mL. Recovery studies were performed using the lyophilized mycelia, with each spiked with sclerotiorin at 1 and 10 mg, respectively. The recoveries were found in the range of 97-109%. The coefficients of variation were …

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Simultaneous Determination Of Febantel, Fenbendazole, Oxfendazole And Oxfendazole Sulfone In Livestock By Matrix Solid Phase Dispersion Extraction Technique And Hplc, S.-C. Su, H.-H. Chou, P.-C. Chang, C.-H. Liu, S.-S. Chou Jul 2020

Simultaneous Determination Of Febantel, Fenbendazole, Oxfendazole And Oxfendazole Sulfone In Livestock By Matrix Solid Phase Dispersion Extraction Technique And Hplc, S.-C. Su, H.-H. Chou, P.-C. Chang, C.-H. Liu, S.-S. Chou

Journal of Food and Drug Analysis

Febantel, fenbendazole, oxfendazole and oxfendazole sulfone in livestock products were simultaneously extracted by traditional and matrix solid phase dispersion (MSPD) method, and then analyzed by high performance liquid chromatography (HPLC). In the traditional method, four benzimidazoles were extracted with acetonitrile under basic conditions, partitioned with n-hexane, and cleaned by a Sep-Pak C18 cartridge. In the MSPD method, samples were blended with Bondesil-C18, and the mixture was packed in a filtration column. This column was connected in tandem with an alumina N cartridge and eluted with acetonitrile. These benzimidazoles were determined by HPLC with a photodiode array detector. Recovery tests of …

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Aqueous Extract From Taiwanese Agrocybe Cylindracea Strain B Protects Dna Against • Oh- Mediated Strand Breaks, L.-H. Wang, S.-J. Tsai, S.-Y. Lin Jul 2020

Aqueous Extract From Taiwanese Agrocybe Cylindracea Strain B Protects Dna Against • Oh- Mediated Strand Breaks, L.-H. Wang, S.-J. Tsai, S.-Y. Lin

Journal of Food and Drug Analysis

Water extract from Agrocybe cylindracea strain B (ACE), an edible mushroom produced in Taiwan, has previously been studied for its antioxidative activities. ACE is an efficient antioxidant in vitro. Since oxidative alterations of DNA can be linked to the development of cancer, we decided to study whether ACE protects DNA against oxidative stress. We report here that pUC18 plasmid DNA and HepG2 cells' DNA are damaged by • OH radicals generated from Cu (II) plus H2O2. The DNA damage was quantified by determining the diminution of supercoiled DNA forms and the DNA migration after oxidative attack using agarose gel electrophoresis. …

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Determination Of Xanthonolol By High Performance Liquid Chromatography For Pharmacokinetic Studies In Rats, Y.-B. Huang, J.-S. Chang, P.-C. Wu, Y.-H. Tsai Jul 2020

Determination Of Xanthonolol By High Performance Liquid Chromatography For Pharmacokinetic Studies In Rats, Y.-B. Huang, J.-S. Chang, P.-C. Wu, Y.-H. Tsai

Journal of Food and Drug Analysis

A rapid and simple HPLC method was developed for the determination of xanthonolol concentration in plasma. The sample preparation utilized liquid-liquid extraction before injection into the HPLC system. Phenazine was used as the internal standard. Separation was obtained using a reversed-phase column under isocratic conditions. The mobile phase consisted of a 65% McIlvaine buffer containing 0.05% triethylamine adjusted to pH 6.4 with phosphoric acid, 18% acetonitrile, and 17% methanol. Xanthonolol was detected at the ultraviolet wavelength 235 nm. The lower limit of quantization was 50 ng/mL. The assay was applied to a pharmacokinetic study in rats. The plasma concentration of …

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Anodic Polarographic Determination Of Lansoprazole And Omeprazole In Pure Form And In Pharmaceutical Dosage Forms, F. Belal, N. El-Enany, M. Rizk Jul 2020

Anodic Polarographic Determination Of Lansoprazole And Omeprazole In Pure Form And In Pharmaceutical Dosage Forms, F. Belal, N. El-Enany, M. Rizk

Journal of Food and Drug Analysis

Lansoprazole (LNS) and omeprazole (OMP) are therapeutically important drugs, and the need arose for a simple, reliable method for their content uniformity of their tablets. The anodic polarographic behavior of lansoprazole and omeprazole has been studied in Britton-Robinson buffer (BRb) over the pH range 4.1-11.5. In BRb of pH 7 well-defined anodic waves were produced with diffusion-current constant (Id) of 1.70 ± 0.01 (n = 6) and 1.66 ± 0.01 (n = 8) for LNS and OMP, respectively. The current-concentration plots were rectilinear over the ranges of 4-24, 2-16 μg mL-1 using Direct Current (DCt) mode for LNS and OMP, …

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Determination Of Methyl- And Inorganic Mercury In Fish Using Focused Microwave Digestion Followed By Cu++ Addition, Sodium Tetraethylborate Derivatization, N-Heptane Extraction, And Gas Chromatography-Mass Spectrometry, S.-S. Chen, S.-S. Chou, D.-F. Hwang Jul 2020

Determination Of Methyl- And Inorganic Mercury In Fish Using Focused Microwave Digestion Followed By Cu++ Addition, Sodium Tetraethylborate Derivatization, N-Heptane Extraction, And Gas Chromatography-Mass Spectrometry, S.-S. Chen, S.-S. Chou, D.-F. Hwang

Journal of Food and Drug Analysis

The analytical procedure for analysis of methyl- and inorganic mercury in fish was developed in this study. It involved microwave-assisted digestion with alkaline solution (tetra methyl ammonium hydroxide), addition of Cu++, aqueous-phase derivatization of mercury species with sodium tetraethylborate, and subsequent extraction with n-heptane. The various mercury derivatives were desorbed in the splitless injection port of a gas chromatograph and subsequently analyzed by electron impact mass spectrometry. Optimum conditions allowed the sample throughout to be controlled by the instrumental analysis time (about 8 min per sample) but not by the sample preparation step. At the irradiation power of 15-30, 45, …

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Detection Of Lactoferrin In Bovine And Goat Milk By Enzyme-Linked Immunosorbent Assay, P.-W. Chen, F.C. Mao Jul 2020

Detection Of Lactoferrin In Bovine And Goat Milk By Enzyme-Linked Immunosorbent Assay, P.-W. Chen, F.C. Mao

Journal of Food and Drug Analysis

Lactoferrin (LF) has been proposed as a multifaceted functional ingredient of food. A competitive enzyme-linked immunosorbent assay (ELISA) was established to determine the LF in milk by using bovine LF antiserum and bovine LF-Biotin conjugate. The LF concentration in cow milk samples could be determined by this ELISA. However, the LF concentration in goat milk could not be measured using this method. Thus, the goat LF ELISA was established using goat LF-Biotin conjugate to measure the LF concentration in goat milk. Milk samples were collected for measurement of their LF concentrations. The mean LF level for the individual cow milk …

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Formononetin - An Isoflavone Metabolite Found In The Liver Of Rats Fed With Soy Protein Isolate, H.C. Chang, M. Laly, R.L. Prior, T.M. Badger Jul 2020

Formononetin - An Isoflavone Metabolite Found In The Liver Of Rats Fed With Soy Protein Isolate, H.C. Chang, M. Laly, R.L. Prior, T.M. Badger

Journal of Food and Drug Analysis

Soy protein isolate, containing genistein, daidzein and glycitein, was used to study the profiles of soy isoflavone metabolites in Sprague-Dawley rats. After 50 days, rat liver tissues, serum and urine were analyzed by HPLC-MS and HPLC-ECD. HPLC-APCI-MS results showed that daidzein, equol, genistein and O-DMA were detected. Interestingly, the aglycone and the conjugated forms of formononetin, which is a methylated derivative of daidzein found in many plants, were also observed in rat liver and serum, but not in urine. The respective concentrations of these two forms of formononetin were 0.056 ± 0.022 and 0.621 ± 0.116 nmoL/g (mean ± SD) …

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Establishment Of Characteristic Fingerprint Chromatogram For The Identification Of Chinese Herbal Medicines, S.-K. Wong, S.-K. Tsui, S.-Y. Kwan, X.-L. Su, R.-C. Lin, L.-M. Tang, J.-X. Chen Jul 2020

Establishment Of Characteristic Fingerprint Chromatogram For The Identification Of Chinese Herbal Medicines, S.-K. Wong, S.-K. Tsui, S.-Y. Kwan, X.-L. Su, R.-C. Lin, L.-M. Tang, J.-X. Chen

Journal of Food and Drug Analysis

Despite the fact that chromatographic fingerprint analysis has been used increasingly for the identification of Chinese herbal medicines, there is no recognized procedure for the establishment of characteristic fingerprint chromatogram. In this paper, a chromatographic response function (CRF) was introduced to quantitatively compare chromatograms obtained under different experimental conditions. As a result, the optimum experimental conditions for the establishment of characteristic fingerprint chromatogram could be identified. Moreover, since the proposed function involved data that were directly available from the workstation after each chromatographic analysis, calculation could be automated. In this paper, the function was successfully applied to obtain characteristic fingerprint …

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Comparison Of The Effects Of "Hot" And "Cold" Chinese Medicinal Plants On The Production Of Inflammatory Mediators By Raw 264.7 Cells, S.-C. Ho, P.-J. Tsai Jul 2020

Comparison Of The Effects Of "Hot" And "Cold" Chinese Medicinal Plants On The Production Of Inflammatory Mediators By Raw 264.7 Cells, S.-C. Ho, P.-J. Tsai

Journal of Food and Drug Analysis

The hot/cold balance system plays an important therapeutic role in traditional Chinese medicine and is applied in food selection by East Asians. Foods traditionally considered "hot" have been demonstrated to have the ability to enhance basal prostaglandin E2 production Conversely, foods considered "cold" can suppress prostaglandin E2 production in lipopolysaccharide (LPS)-stimulated cells. Therefore, this study attempted to further confirm the previous hypotheses by selecting four Chinese medicinal plants and comparing their natural hot or cold characteristic influences on the production of inflammatory mediators. Herbs traditionally regarded as hot (Coptis chinensis and Scutellaria baicalensis) or cold (Zingiber officinale and Cinnamomum cassia) …

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Liquid Chromatographic Method For Determination Of Calcium Pantothenate Preparations And Related Stability Studies, T.-M. Wang, Y.-M. Chiu, L.-C. Lin, M.-C. Hsu Jul 2020

Liquid Chromatographic Method For Determination Of Calcium Pantothenate Preparations And Related Stability Studies, T.-M. Wang, Y.-M. Chiu, L.-C. Lin, M.-C. Hsu

Journal of Food and Drug Analysis

This report describes a fast, simple and reliable HPLC method for the assay and quantitative determination of calcium pantothenate in commercial products. The samples were analyzed on a C18 column with the mobile phase of acetonitrile and potassium dihydrogen phosphate solution (adjusted pH = 2.5 with phosphoric acid) at a flow rate of 1.0 mL/min and UV absorbance defection at 204 nm. Ampicillin was used as an internal standard. The retention times of calcium pantothenate and ampicillin were 5.3 and 6.5 min, respectively. An equation was presented for linear relationship between peak height ratios of calcium pantothenate to ampicillin and …

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Determination Of Major Carotenoids In Human Serum By Liquid Chromatography, M.-S. Tzeng, F.-L. Yang, G.-S. Wang-Hsu, B.-H. Chen Jul 2020

Determination Of Major Carotenoids In Human Serum By Liquid Chromatography, M.-S. Tzeng, F.-L. Yang, G.-S. Wang-Hsu, B.-H. Chen

Journal of Food and Drug Analysis

A simple and rapid high performance liquid chromatographic (HPLC) method was developed to determine the major carotenoids in human serum. Lutein, β-carotene and lycopene were separated within 20 min using a C18 column and a mobile phase of methanol/methylene chloride (95:5, v/v) with sample solvent methanol/methylene chloride (45:55, v/v), detection at 476 nm and flow rate at 0.8 mL/min. A high recovery was achieved with this method, which amounted to 92, 90 and 87% for lutein, β -carotene and lycopene, respectively, while the detection limit was 5.0, 10.0 and 4.0 ng/mL. This method was applied to determine the major carotenoids …

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Evaluation Of Total Antioxidant Activity Of Several Popular Vegetables And Chinese Herbs: A Fast Approach With Abts/H2o2/Hrp System In Microplates, I.-C. Chen, H.-H. Chang, H.-W. Yang, G.-L. Chen Jul 2020

Evaluation Of Total Antioxidant Activity Of Several Popular Vegetables And Chinese Herbs: A Fast Approach With Abts/H2o2/Hrp System In Microplates, I.-C. Chen, H.-H. Chang, H.-W. Yang, G.-L. Chen

Journal of Food and Drug Analysis

Total antioxidant activities for several popular vegetables and traditional Chinese herbs were evaluated in an ABTS/H2O2/HRP system with a microplate reader. This improved method offers a rapid and sensitive way to measure total antioxidant activity of testing samples. Most samples had antioxidative capacities. Among the tested vegetables, sugar beet and red cabbage had the highest total antioxidant activity, whereas tropical Almond Terminalia and Moutan Radicis Cortex had exceptionally high total antioxidant activity in the tested Chinese herbs.

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Diverse Effects Of Phytoestrogen Biochanin A On Rat Pituitary Tumor Cells, C.-W. Ying, J.-T. Hsu, Y.-C. Yang Jul 2020

Diverse Effects Of Phytoestrogen Biochanin A On Rat Pituitary Tumor Cells, C.-W. Ying, J.-T. Hsu, Y.-C. Yang

Journal of Food and Drug Analysis

Biochanin A, a nonsteroidal estrogen present in plants, has been suggested in epidemiological studies to be associated with a reduced risk of hormone-related cancers. In this study, we examined the effects of biochanin A on the cell growth and gene expression of an estrogen-responsive pituitary cell line GH3. Results showed that biochanin A inhibited growth of GH3 cells in a dose-dependent manner and at physiologically relevant concentrations. At concentrations of 1 and 5 μg/mL, inhibition of cell growth was observed. The gene expressions of estrogen receptor (ER) mRNA and an estrogen responsive gene, the progesterone receptor (PR), were down-regulated by …

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A Comparative Study Of The Release Of Active Ingredients From Semisolid Cosmeceuticals Measured With Franz, Enhancer Or Flow-Through Cell Diffusion Apparatus, W. Liebenberg, E. Engelbrecht, A. Wessels, B. Devarakonda, W. Yang, Villiers M.M. De Jul 2020

A Comparative Study Of The Release Of Active Ingredients From Semisolid Cosmeceuticals Measured With Franz, Enhancer Or Flow-Through Cell Diffusion Apparatus, W. Liebenberg, E. Engelbrecht, A. Wessels, B. Devarakonda, W. Yang, Villiers M.M. De

Journal of Food and Drug Analysis

This study reports the release properties of the poorly water-soluble drug hydrocortisone, antibacterial agent triclosan, and the water-soluble anti-aging compounds salicylic acid and ascorbic acid from gels, creams, and ointments, alone or combined with triclosan. Drug release was measured with the flow-through, Franz, and Enhancer cell release testing methods. For the 3 methods, a cellulose acetate membrane soaked in lipophilic oleic acid and dissolution mediums composed of a phosphate buffer pH 5.8 or a 50% ethanol: buffer mixture gave more constant and less variable release profiles (similarity factors, f2, above 50). Comparison of the release rates of hydrocortisone, salicylic acid, …

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Analysis Of The Analogues Of Aristolochic Acid And Aristolactam In The Plant Of Aristolochia Genus By Hplc, J.-H. Jou, C.-Y. Li, E.P. Schelonka, C.-H. Lin, T.-S. Wu Jul 2020

Analysis Of The Analogues Of Aristolochic Acid And Aristolactam In The Plant Of Aristolochia Genus By Hplc, J.-H. Jou, C.-Y. Li, E.P. Schelonka, C.-H. Lin, T.-S. Wu

Journal of Food and Drug Analysis

A facile reversed-phase HPLC method for the analysis of aristolochic acid (AA) and aristolactam (AL) analogues was developed and used for the quantitative determination and quality control of the traditional Chinese medicine. Homonymic Chinese crude drugs such as Stephania tetrandra and Aristolochia fanchi Clematis armandii and A. manshuriensis can be easily identified by this method. The quantitative determination of AAs and ALs in 12 species of Aristolochia (A. elegans, A. zollingeriana, A. cucurbitifolia, A. mollis, A. kaempferi, A. shimadii, A. heterophylla, A. debilis, A. foveolata, A. contorta, A. trilobata and A. odoratissima) has been investigated. The chromatograms obtained in this …

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Chemical Identification On The Origins Of Phellodendri Cortex, L.-J. Juang, S.-J. Sheu Jul 2020

Chemical Identification On The Origins Of Phellodendri Cortex, L.-J. Juang, S.-J. Sheu

Journal of Food and Drug Analysis

Twenty-eight samples of commercial articles of Phellodendri Cortex, originating from Phellodendron amurense RUPRECHT, P. chinense SCHNEID, P wilsonii HAYATA et KANEHIRA, and P chinense SCHNEID. var. glabrius culum SCHNEID, were collected from different herbal markets in Taiwan and Mainland China. The five alkaloids berberine, palmatine, jatrorrhizine, phellodendrine, and magnoflorine were determined by capillary electrophoresis for their contents in the samples, thereby a chemical identification method was established for identifying the sources of the drug materials. As this study turned out, P. chinense var. glabrius culum-was found to be the predominant variety prevailing in Taiwan markets owing to their considerably high …

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Detection Of Endotoxin Contamination In Chinese Herbs By Nf-Κb Activity-Based Reporter Assays, Y.-H. Hsu, S.-L. Fu Jul 2020

Detection Of Endotoxin Contamination In Chinese Herbs By Nf-Κb Activity-Based Reporter Assays, Y.-H. Hsu, S.-L. Fu

Journal of Food and Drug Analysis

Severe LPS contamination in drugs and food can cause health problems and occasionally mislead research conclusions. To ensure the quality of traditional Chinese medicinal herbs (TCMH), LPS contamination problem should be evaluated. We here described a NF-κB activity-based reporter assay to detect LPS contamination. We first created a macrophage cell line with integrated reporter gene consisting of NF-κB-responding sites upstream of the luciferase gene. The presence of LPS leads to NF-κB activation, and thus triggers downstream luciferase expression. The specificity of LPS-derived luciferase activity was confirmed by adding the LPS inhibitor, polymyxin B. In our system, the level of LPS …

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Molecular Characterization Of Polysaccharides In Hot-Water Extracts Of Ganoderma Lucidum Fruiting Bodies, Y.-W. Chang, T.-J. Lu Jul 2020

Molecular Characterization Of Polysaccharides In Hot-Water Extracts Of Ganoderma Lucidum Fruiting Bodies, Y.-W. Chang, T.-J. Lu

Journal of Food and Drug Analysis

Polysaccharide components in hot-water extract of Ganoderma lucidum fruiting bodies were separated and their molecular weights and distribution profiles were measured using high-performance size-exclusion chromatography with multi-angle laser light scattering detection (HPSEC-MALLS). Three polysaccharide groups were found in the extract with a weight percentage of 31, 46 and 23%, respectively. The weight-average molecular weights of these groups were 2.08 × 106, 2.3 × 104 and 1.2 × 104 g/mol, respectively. The representative fractions of these 3 groups were obtained using differential precipitation with ethanol at 20, 60 and 80% (v/v) concentrations for further property study. The first group that had …

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Erratum: (Journal Of Food And Drug Analysis Vol. 11 (3) (177-182)), Skip Jul 2020

Erratum: (Journal Of Food And Drug Analysis Vol. 11 (3) (177-182)), Skip

Journal of Food and Drug Analysis

[No abstract available]

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