Digital Commons Network^™

Prevalence Of Dirofilaria Immitis In Southern California’S Invasive Aedes Species And Native Culiseta Incidens, Zaina Chaban

University of the Pacific Theses and Dissertations

Dirofilaria immitis is a mosquito-borne parasite that causes dog heartworm disease, effecting over 100,000 dogs in the United States each year (CAPC - Parasite Prevalence Maps., 2021). Increased disease reports are commonly attributed to an increase in the local mosquito vector population. Since 2013, dog heartworm disease cases have increased by approximately 21% in the United States as reported by the American Heartworm Society. During the same time, invasive mosquitoes belonging to the genus Aedes have been found in Southern California and spread to other areas. The purpose of our research was to determine the prevalence of Dirofilaria …

Novel Mechanisms Of Nitric Oxide Activation In The Kidney, Randee Sedaka

All ETDs from UAB

Globally, adult sodium consumption has far exceeded daily intake recommendations, leading to one in ten cardiovascular deaths. Given the rise in hypertension prevalence, uncontrolled hypertension with antihypertensive medication use, and significant associations between high salt intake and high blood pressure, there is a need to better understand the mechanisms involved in hypertension. Nitric oxide synthase 1 (NOS1) is a well-established regulator of fluid and electrolyte balance in the renal collecting duct (CD) with highest activity in the inner medulla (IM). We previously reported that high salt (HS) intake stimulates CD endothelin B receptor (ETBR)/NOS1-dependent generation of NO, thereby inhibiting the …

Diurnal Dysfunction In Control Of Sodium Excretion In Diet-Induced Obesity, Reham Soliman

All ETDs from UAB

The renal endothelin system plays a key role in sodium excretion, particularly under high salt (HS) diet. HS stimulates renal endothelin-1 (ET-1), which binds and activates the endothelin receptor B (ETB) to excrete the excess salt. Loss of ETB receptor is linked to the development of hypertension under HS. Central clock genes are important in maintaining rhythmic patterns of sodium excretion and ET-1 is identified as a target for clock genes, including Period and Bmal1. ET-1 excretion follows a diurnal rhythm along with sodium excretion. Sexual dimorphism is evident in the diurnal regulation of endothelin-mediated natriuresis and its downstream targets. …

Mitochondrial Priming Of Tumor Initiating Cells Involves Repression Of Mitochondrial Fission Activity Towards Redox Regulation Of The Cell Cycle Regulator Cyclin E, Brian Spurlock

All ETDs from UAB

Mitochondrial dynamics primarily refers to the opposing fission or fusion events between individual mitochondria, which maintains steady-state mitochondrial morphology in cells. Fission and fusion activities maintain the energetics of normal and neoplastic stem cells. We developed the novel approach mito-SinCe2 to study the bidirectional relationship between mitochondrial dynamics and energetics. Application of this method led to the hypothesis that mitochondria-dependent ovarian tumor initiating cells interconvert between 3 states with distinct mitochondrial energetics/dynamics relationships. Cells in one state, characterized by high mitochondrial ROS and low fission activity, appear to be primed for self-renewal by mitochondria. We call these cells mitochondria-primed stem …

Pheochromocytoma: A Systematic Review Of The Diagnosis, Treatment, And Risk Factors, Brandon A. Singletary

All ETDs from UAB

ABSTRACT Pheochromocytomas and extra-adrenal paragangliomas (PGLs) are forms of neuroendocrine tumors which when left untreated lead to debilitating complications of cerebral hemorrhaging, stroke, and death. Typically, the tumors present with symptoms of episodic headaches, sweating, and palpitations, however hypertension is considered one of the more predominant signs. As a result of the rarity of the disease and wide range of symptoms, there are often difficulties in screening and diagnosis to identify these tumors. While there are many case studies and results in small populations, large epidemiologic driven studies are limited. To date the majority of large-scale studies exist in countries …

Toxin Secretion By Mycobacterium Tuberculosis, Uday Tak

All ETDs from UAB

Mycobacterium tuberculosis (Mtb) is a highly infectious airborne bacterial pathogen, and the causative agent of human tuberculosis. Mtb infects approximately one-third of the human population, and is responsible for more deaths than any other infectious disease in the history of mankind. Mtb is an intracellular pathogen and primarily infects myeloid cells, such as macrophages in the human lung. In macrophages, Mtb establishes a replication niche within membrane-bound vacuoles named phagosomes, while subverting immunity. The intracellular survival of Mtb depends on manipulation of host cellular processes through secreted effector proteins. Ultimately, this intracellular vacuole is ruptured by the type VII secretion …

Uav Navigation, Tracking, And Interception Using Deep Reinforcement Learning, Ali Alhaj Darwish

All ETDs from UAB

We propose a self-supervised, model-free deep reinforcement learning architecture with cascade reward for unmanned aerial vehicle navigation and target interception in a 3D environment. The first contribution of the dissertation solves the problem of partial observability when non-linear function approximators are used for learning stochastic policies. The second contribution optimizes the problem of maximizing the total expected rewards. The third contribution trains the agent in a photo-realistic environment with a real physics engine. To achieve these goals, a deep Q-network that combines double and dueling architectures is adopted as a value function approximator, and the prioritized experience replays the sample …

Creatinine Detection Based On Molecularly Imprinted Technique And Luminescence Of Nano-Diamond As A Signal Transducer, Reim Almotiri

All ETDs from UAB

Creatinine measurement is an important diagnostic test for assessing kidney health. This work represents our current progress in creatinine artificial sensor based on a molecularly imprinted polymer of polyacrylamide hydrogel and nanodiamonds fluorescence change. For a 0.3 mM creatinine rebinding solution, an imprinting factor of 2.54 was measured. Also, a molecularly imprinted polymer was obtained by incorporating nanodiamond into a creatinine-imprinted polyacrylamide hydrogel. The quenching of peak nanodiamond fluorescence was significantly higher in the creatinine-imprinted polymer compared to the non-imprinted polymer, indicative of higher creatinine affinity in the imprinted polymer. Nanodiamond fluorescence from nitrogen-vacancy color centers (NV- and NV0) was …

Mid-Fusiform Sulcus Anatomy And Its Relationship To Face Processing And Social Functioning In Autism Spectrum Disorder, Carla Ammons

All ETDs from UAB

Autism Spectrum Disorder (ASD) is a neurodevelopmental disorder characterized by deficits in social communication and restricted and repetitive behaviors and interests. One of the most consistent findings in ASD is reduced attention to and atypical processing of faces. However, the brain basis of aberrant face processing in ASD remains opaque. A novel neuroanatomical feature, the mid-fusiform sulcus (MFS), is strongly associated with face-selective regions of the fusiform gyrus (FG) as well as other large-scale functional and structural divisions in the ventral temporal cortex (Weiner et al., 2014). However, this feature is unexplored in ASD. The overarching goal of this dissertation …

Prediction Of The Optimal Catheter Placement Selection For Therapy Infusion To Treat Brain Tumors, Lisa Hope Antoine

All ETDs from UAB

ABSTRACT In 2020, the National Brain Tumor Society reported that physicians would diagnose more than 87,000 new cases of brain tumors. These cases will add to the approximately 700,000 individuals in the United States living with brain and central nervous system tumors. Almost 18,000 of these cases will prove fatal [1]. The blood-brain barrier (BBB) hinders the effective treatment of brain tumors. The task of this barrier is to protect the brain from composition instabilities in blood plasma and shield the brain from agents that may adversely impact brain functionality. However, by performing its tasks the barrier prevents potential effective …

Understanding The Impact Of Diet And Obesity On Anti-Tumor Immunity And Therapeutic Efficacy In Kidney Cancer, Rachael Miller Orlandella

All ETDs from UAB

Obesity is regarded as a major risk factor for developing renal cell carcinoma (RCC). Advanced-stage RCC exhibits chemotherapeutic resistance, but is responsive to immunotherapies such as the immune checkpoint inhibitor anti-programmed cell death-1 (anti-PD-1). Despite some clinical successes, response rates remain low for anti-PD-1 monotherapy (20-30%). Preclinical evaluations of immunotherapeutic strategies typically use lean mice and do not account for patient comorbidities. This may explain the underwhelming success rates following clinical translation of new cancer treatments. Recent studies found that obesity was associated with favorable outcomes and responses to immunotherapy in melanoma. However, the effects of obesity on anti-tumor immunity …

Role Of Trib3 In Progression And Pathogenesis Of Diabetic Retinopathy, Priyamvada Milind Pitale

All ETDs from UAB

Diabetic retinopathy (DR) is reported to be one of the leading causes of blindness in the United States by Center for Disease Prevention and Control. As the estimated prevalence of the disease will likely triple by 2020, the research in this area should focus on identifying novel targets and therapeutic approaches. One of the therapeutic approaches for DR is the reprograming of retinal metabolism to delay the progression of the disease. The unfolded protein response (UPR) is recognized as a cellular pathway activated in diabetic retina which interacts with key transcription factors to physiologically regulate glucose, lipid homeostasis, and angiogenic …

Novel Hiv Adaptation Strategy Against Host Cd8 T Cell Immunity, Kai Qin

All ETDs from UAB

Since the 1980s, HIV infection has caused millions of death and tremendous economic losses worldwide. Despite great scientific advances, the only effective therapy to date, antiretroviral therapy (ART), merely delays disease progression yet cannot eradicate viral infection. Therefore, an effective HIV vaccine that can prevent the acquisition of infection would be a desirable alternative. The critical role of CD8 T-cell immunity in HIV infection has been well documented and therefore not surprisingly a great deal of effort has been focused on developing an effective CD8 T-cell based HIV vaccine. However, by far, such CD8 T-cell based HIV vaccine trials have …

An Investigation Of Ion Bombardment From Microwave Plasma Chemical Vapor Deposition During Bias Enhanced Nucleation Of Boron-Rich Materials, Bhavesh Ramkorun

All ETDs from UAB

This study involves an Ar/H2 plasma acting on a negatively DC-biased silicon sub-strate in a microwave plasma chemical vapor deposition. The flow rate of hydro-gen was constant at 500 sccm, and that of argon was varied between 0 sccm and 500 sccm. Bias current was recorded for several values of the applied bias voltage ranging from – 100V to – 340V. At 10 and 20 torr, a peak in the bias current was found as argon flow rate changed. A decrease in current with varied argon intensi-ty in glow discharge plasma has been reported in the literature. Our results sub-stantiate …

Novel Methods For Image Analysis To Evaluate Relationships Between Biomarkers In Alzheimer's Disease, Fabio Raman

All ETDs from UAB

Neuroimaging biomarkers, including pathological amyloid and tau measured with positron emission tomography (PET) and neurodegeneration measured with magnetic res-onance (MR), are part of the research framework for Alzheimer’s disease (AD). Abnormal brain amyloid is currently the earliest established phenotypic marker of AD, and other imaging biomarkers such as tau-PET become abnormal later in the progression of AD pathophysiology. The ability to measure amyloid and predict both tau status and cogni-tion using a single amyloid-PET study through a method readily employed in both re-search and clinical settings would provide a valuable tool in assessing AD. The first goal of this dissertation …

Investigating Growth-Related Hormones And Their Roles In Metabolism In Fish Skeletal Muscle, Ross Michael Lee Reid

All ETDs from UAB

The endocrine system is essential for the maintenance and regulation of skeletal muscle growth and metabolic processes, as secretions of growth-related hormones directly regulate these systems. The roles of these growth-related hormones are well understood in mammals yet are not well defined in teleosts. Thus, the work outlined in these chapters provides novel insight for the actions of two growth-related hormones, Growth Hormone (GH) and Teneurin C-terminal Associated Peptide (TCAP), in fish skeletal muscle. First, Chapter 2 highlights the work expanding the application of resazurin as a novel method of in vivo metabolic assessment across fish life-stages, which was utilized …

Investigating The Roles Of The Renal Primary Cilium Through The Use Of Intravital Imaging, Dustin Zachary Revell

All ETDs from UAB

Primary cilia are small microtubule based appendages present on nearly every cell type in the mammalian body, and are highly conserved from organisms as simple as algae to humans. Cilia function as complex signaling centers integrating a variety of pathways and play critical roles throughout development and in maintaining homeostasis. Mutations to ciliary proteins in humans result in a wide array of diseases termed ciliopathies, which have a broad range of symptoms affecting nearly every organ in the body. Renal primary cilia are present on the apical side of tubular epithelium, where they project into the lumen and are thought …

[email protected]: Using The Tech Start-Up Concept To Train, Engage, And Inform Students, Stephanie J. Coopman, Ted Coopman

Faculty Publications

Undergraduate and graduate students were enrolled in an upper-division online experiential learning course organized as a technology company start up at a public university in the US. Students participated in an academic department’s social media team, publishing a weekly newsletter and producing and curating content for multiple social media outlets designed for public and university audiences, a website for the department’s students, and a career portal. Responses to survey questions provided support for Experiential Learning Theory’s cyclical learning model. In addition, students viewed the entrepreneurial approach to the team as both liberating and challenging as they engaged with each other …

Reduced Dataset Neural Network Model For Manuscript Character Recognition, Mohammad Anwarul Islam

College of Graduate Studies: Theses & Dissertations

The automatic character recognition task has been of practical interest for a long time. Nowadays, there are well-established technologies and software to perform character recognition accurately from scanned documents. Although handwritten character recognition from the manuscript image is challenging, the advancement of modern machine learning techniques makes it astonishingly manageable. The problem of accurately recognizing handwritten character remains of high practical interest since a large number of manuscripts are currently not digitized, and hence inaccessible to the public. We create our repository of the datasets by cropping each letter image manually from the manuscript images. The availability of datasets is …

Perceptions Of Physical Activity Engagement Among African American Emerging Adults At Georgia Southern University - Statesboro Campus, Addison Mickens

College of Graduate Studies: Theses & Dissertations

Introduction: Physical Activity (PA) is critical to the health and well-being of African American emerging adults, ages 18-25. Research suggests that African American (AA) college students engage in less PA than other racial/ethnic groups. Few studies have examined the relationship between the collegiate built environment (CBE) impact on PA in this population. The Social Ecological Model (SEM) and the Social Cognitive Theory (SCT) were used to show how the environment facilitates influence on the behavior through physical and social factors affecting the behavior of engaging in PA. Methods: The purpose of this cross-sectional, concurrent multi-method study was to determine barriers …

2020 Fall Commencement, Georgia Southern University

Commencement Programs

No abstract provided.

Closed Adoption: An Illusory Promise To Birth Parents And The Changing Landscape Of Sealed Adoption Records, Bryn Baffer

Catholic University Journal of Law and Technology

Imagine spitting into a tube and mailing your DNA off only to discover that you had a sibling who had been adopted by another family or that a parent’s affair had resulted in a half-sibling. For many individuals, these family secrets have been exposed due to direct-to-consumer DNA testing companies, such as 23andMe.

By the 1950s, most states had enacted statutes that sealed adoption record files in order to preserve the privacy of the birth parents, adoptees, and adoptive families. While some states have moved toward granting adoptees access to their adoption records, most states still have some type of …

Common Data Set, 2019-2020, Planning, Research, And Policy Analysis

Common Data Set (2001-present)

Common Data Set 2019-2020 for Illinois State University

Predicting Complex System Behavior Using Hybrid Modeling And Computational Intelligence, Vinayaka Gude

Doctoral Dissertations

“Modeling and prediction of complex systems is a challenging problem due to the sub-system interactions and dependencies. This research examines combining various computational intelligence algorithms and modeling techniques to provide insights into these complex processes and allow for better decision making. This hybrid methodology provided additional capabilities to analyze and predict the overall system behavior where a single model cannot be used to understand the complex problem. The systems analyzed here are flooding events and fetal health care. The impact of floods on road infrastructure is investigated using graph theory, agent-based traffic simulation, and Long Short-Term Memory deep learning to …

Studies On The Stability Of Plasma Proteins And Their Fragments, Paul Praveen Nakka

Doctoral Dissertations

“The stability of a protein-based biological drug is an important concern during processing, storage or patient administration. There are various forces which contribute to the stabilizing of proteins. Both hydrogen bonds and hydrophobic interactions play an important role in protein stability. Improper folding or misfolding of proteins leads to aggregation, which is a major problem found in the bioprocessing industry. The objectives of this research are: 1) to determine the effect of environmental factors such as pH, temperature, additives or salts, and cosolvents on the stability human serum albumin (HAS), which is the most abundant protein in plasma, 2) to …

Magnetic Control Of Transport Of Particles And Droplets In Low Reynolds Number Shear Flows, Jie Zhang

Doctoral Dissertations

“Magnetic particles and droplets have been used in a wide range applications including biomedicine, biological analysis and chemical reaction. The manipulation of magnetic microparticles or microdroplets in microscale fluid environments is one of the most critical processes in the systems and platforms based on microfluidic technology. The conventional methods are based on magnetic forces to manipulate magnetic particles or droplets in a viscous fluid.

In contrast to conventional magnetic separation method, several recent experimental and theoretical studies have demonstrated a different way to manipulate magnetic non-spherical particles by using a uniform magnetic field in the microchannel. However, the fundamental mechanism …

Fuzzy Logistic Regression For Detecting Differential Dna Methylation Regions, Tarek M. Bubaker Bennaser

Doctoral Dissertations

“Epigenetics is the study of changes in gene activity or function that are not related to a change in the DNA sequence. DNA methylation is one of the main types of epigenetic modifications, that occur when a methyl chemical group attaches to a cytosine on the DNA sequence. Although the sequence does not change, the addition of a methyl group can change the way genes are expressed and produce different phenotypes. DNA methylation is involved in many biological processes and has important implications in the fields of biomedicine and agriculture.

Statistical methods have been developed to compare DNA methylation at …

Tools To Evaluate Nanodiamond-Mediated Delivery Of Tiopronin For Cataract Prevention, Justin Beltz

Doctoral Dissertations

”There is a growing demand for non-surgical means of cataract treatment. This dissertation presents three bodies of work that reflect the early-stage development of eye drop formulations aimed at delaying cataract progression. These formulations consist of the antioxidant 2-mercaptopropionylglycine (MPG) loaded onto nanodiamond particles.

Cataractogenesis is linked to oxidative damage to lens proteins. To investigate the potential of MPG for protection against oxidative damage, A549 cells were incubated in 0.6 mM tert-butylhydroperoxide (tBHP). Cells exposed to tBHP without MPG exhibited elevated levels of reactive oxygen species, which led to the depletion of the vital antioxidant glutathione and, ultimately, apoptosis. Co-administration …

Strategies To Improve The Electrochemical Performance Of Lithium-Ion Batteries By Stabilizing The Interface Of Electrode/Electrolyte, Ye Jin

Doctoral Dissertations

”Lithium-ion batteries (LIBs) provide great potential for electric vehicles, and smart grids as future energy-storage devices. However, there are many challenges in the development of the LIB industry, including low energy and power density of electrode materials, poor rate performance, short cycle life of electrode materials, and safety issues caused by the flammability of the conventional organic liquid electrolytes.

In this research, we were committed to using general approach to efficiently and economically synthesize or modify LIB materials by stabilizing the interface between electrode and electrolyte. Atomic layer deposition (ALD) method was used to coat metal oxide thin films on …

Continuous-Flow Synthesis Of Fine Chemicals And Pharmaceutical Compounds Over Intelligent Organocatalysts With Bifunctional Reactivity, Abdo-Alslam Alwakwak

Doctoral Dissertations

“Many biological systems that utilize organic active sites to catalyze reactions under mild conditions invoke cooperative catalytic pathways, whereby two or more active sites work together to activate the reactant(s). The use of cooperative (bifunctional) catalysts and continuous flow chemistry (a reaction within the narrow channels of a micro‐ or microfluidic reactor) are commonplace in sustainable chemical transformation and attract a great deal of interest with respect to economic and environmentally-sustainable production of fine chemicals, pharmaceuticals, and agrochemicals, water treatment, as well as upgrading of biomass feedstocks. Although, some methods have been developed for immobilization of bifunctional catalysts for cooperative …