Digital Commons Network^™

Ms-275, A Class 1 Histone Deacetylase Inhibitor Augments Glucagon-Like Peptide-1 Receptor Agonism To Improve Glycemic Control And Reduce Obesity In Diet-Induced Obese Mice, Shilpak Bele, Shravan Babu Girada, Aramita Ray, Abhishek Gupta

Open Access archive

Given its glycemic efficacy and ability to reduce the body weight, glucagon-like peptide 1 receptor (GLP-1R) agonism has emerged as a preferred treatment for diabetes associated with obesity. We here report that a small-molecule Class 1 histone deacetylase (HDAC) inhibitor Entinostat (MS-275) enhances GLP-1R agonism to potentiate glucose-stimulated insulin secretion and decrease body weight in diet-induced obese (DIO) mice. MS-275 is not an agonist or allosteric activator of GLP-1R but enhances the sustained receptor-mediated signaling through the modulation of the expression of proteins involved in the signaling pathway. MS-275 and liraglutide combined therapy improved fasting glycemia upon short-term treatment and …

Magnetoelectric Coupling In Bismuth Ferrite—Challenges And Perspectives, N. V. Srihari, K. B. Vinayakumar, K. K. Nagaraja

Open Access archive

Multiferroic materials belong to the sub-group of ferroics possessing two or more ferroic orders in the same phase. Aizu first coined the term multiferroics in 1969. Of late, several multiferroic materials’ unique and robust characteristics have shown great potential for various applications. Notably, the coexisting magnetic and electrical ordering results in the Magnetoelectric effect (ME), wherein the electrical polarization can be manipulated by magnetic fields and magnetization by electric fields. Currently, more significant interests lie in significantly enhancing the ME coupling facilitating the realization of Spintronic devices, which makes use of the transport phenomenon of spin-polarized electrons. On the other …

Serotonin 2a (5-Ht2a) Receptor Affects Cell–Matrix Adhesion And The Formation And Maintenance Of Stress Fibers In Hek293 Cells, Joe Anand Kumar John Jayakumar, Mitradas M. Panicker, Basudha Basu

Open Access archive

5-HT2A, a G-protein coupled receptor, is widely expressed in the human body, including in the gastrointestinal tract, platelets and the nervous system. It mediates various functions, for e.g. learning, memory, mood regulation, platelet aggregation and vasoconstriction, but its involvement in cell-adhesion remains largely unknown. Here we report a novel role for 5-HT2A in cell–matrix adhesion. In HEK293 cells, which are loosely adherent, expression and stimulation of human or rat 5-HT2A receptor by agonists such as serotonin or 2,5-dimethoxy-4-iodoamphetamine (DOI) led to a significant increase in adhesion, while inhibition of 5-HT2A by antipsychotics, such as risperidone, olanzapine or chlorpromazine prevented it. …

Mazef-Rifampicin Interaction Suggests A Mechanism For Rifampicin Induced Inhibition Of Persisters, Cyrus Alexander, Ankeeta Guru, Pinkilata Pradhan, Sunanda Mallick

Open Access archive

Background: Persistence is a natural phenomenon whereby a subset of a population of isogenic bacteria either grow slow or become dormant conferring them with the ability to withstand various stresses including antibiotics. In a clinical setting bacterial persistence often leads to the recalcitrance of various infections increasing the treatment time and cost. Additionally, some studies also indicate that persistence can also pave way for the emergence of resistant strains. In a laboratory setting this persistent phenotype is enriched in nutritionally deprived environments. Consequently, in a batch culture the late stationary phase is enriched with persistent bacteria. The mechanism of persister …

Berberine, A Natural Alkaloid Sensitizes Human Hepatocarcinoma To Ionizing Radiation By Blocking Autophagy And Cell Cycle Arrest Resulting In Senescence, Gautham Ramesh, Shubhankar Das, Satish Rao Bola Sadashiva

Open Access archive

Objective: To study the radiosensitizing potential of Berberine and the underlying mechanism in human hepatocarcinoma (HepG2) cells. Methods: HepG2 cells were challenged with X-rays in combination with Berberine treatment and several in vitro assays were performed. Alteration in cell viability was determined by MTT assay. Changes in intracellular ROS levels, mitochondrial membrane potential/mass, intracellular acidic vesicular organelles as well as cell cycle arrest and apoptotic cell death were analysed by flow cytometry. Induction of autophagy was assessed by staining the cells with Monodansylcadaverine/Lysotracker red dyes and immunoblotting for LC3I/II and p62 proteins. Phase-contrast/fluorescence microscopy was employed to study mitotic catastrophe …

Association Between Serum Micronutrient Levels And Febrile Seizures Among Febrile Children In Southern India: A Case Control Study, Lakmeel Kannachamkandy, Sowmini P. Kamath, Prasanna Mithra, K. Jayashree

Open Access archive

Background: Febrile seizures are prevalent in children. Interactions between the neurotransmitters and micronutrients levels in the biological fluids have been implicated in its occurrence. Objective: To assess the serum copper (Cu), magnesium (Mg) and zinc (Zn) levels in febrile children and to evaluate their association with febrile seizures. Methods: The study included 35 children with febrile seizures as cases and 35 children with febrile illnesses without seizures as controls. Children between six months to five years were included in the study group. The serum Cu, Mg and Zn levels were analyzed by the semi-auto analyzer. Data analysis was done using …

Large-Scale Genome Sequencing Of Mycorrhizal Fungi Provides Insights Into The Early Evolution Of Symbiotic Traits, Shingo Miyauchi, Enikő Kiss, Alan Kuo, Elodie Drula, Annegret Kohler, Marisol Sánchez-García, Emmanuelle Morin, Bill Andreopoulos, Kerrie W. Barry, Gregory Bonito, Marc Buée, Akiko Carver, Cindy Chen, Nicolas Cichocki, Alicia Clum, David Culley, Pedro W. Crous, Laure Fauchery, Mariangela Girlanda, Richard D. Hayes, Zsófia Kéri, Kurt Labutti, Anna Lipzen, Vincent Lombard, Jon Magnuson, François Maillard, Claude Murat, Matt Nolan, Robin A. Ohm, Jasmyn Pangilinan, Maíra De Freitas Pereira, Silvia Perotto, David Hibbett

Biology

Mycorrhizal fungi are mutualists that play crucial roles in nutrient acquisition in terrestrial ecosystems. Mycorrhizal symbioses arose repeatedly across multiple lineages of Mucoromycotina, Ascomycota, and Basidiomycota. Considerable variation exists in the capacity of mycorrhizal fungi to acquire carbon from soil organic matter. Here, we present a combined analysis of 135 fungal genomes from 73 saprotrophic, endophytic and pathogenic species, and 62 mycorrhizal species, including 29 new mycorrhizal genomes. This study samples ecologically dominant fungal guilds for which there were previously no symbiotic genomes available, including ectomycorrhizal Russulales, Thelephorales and Cantharellales. Our analyses show that transitions from saprotrophy to symbiosis involve …

Investigation Of The Antifungal And Anti-Aflatoxigenic Potential Of Plant-Based Essential Oils Against Aspergillus Flavus In Peanuts, Premila Narayana Achar, Pham Quyen, Emmanuel C. Adukwu, Abhishek Sharma, Huggins Zephaniah Msimanga, Hanumanthu Nagaraja, Marikunte Yanjarappa Sreenivasa

Faculty Articles

© 2020 by the authors. Licensee MDPI, Basel, Switzerland. Aspergillus species are known to cause damage to food crops and are associated with opportunistic infections in humans. In the United States, significant losses have been reported in peanut production due to contamination caused by the Aspergillus species. This study evaluated the antifungal effect and anti-aflatoxin activity of selected plant-based essential oils (EOs) against Aspergillus flavus in contaminated peanuts, Tifguard, runner type variety. All fifteen essential oils, tested by the poisoned food technique, inhibited the growth of A. flavus at concentrations ranging between 125 and 4000 ppm. The most effective oils …

Graduate Internship Report- Corcoran High School, Viktoria Granicy

Agricultural Education: Graduate Internship Reports

This internship and project report includes validation documents required in meeting the quality criteria for secondary-level programs of instruction in agriculture. The supporting material includes information to receive state and local funding, outline the goals and objectives of the program, along with an overview of Corcoran High School, the agriculture program, and the community.

Discerning Competitive Vs. Facilitative Relationships Of Weeds On Forest Restoration Efforts At La Sal Del Rey, Tx, Mylen A. Arias

Theses and Dissertations

Dryland ecosystems need effective restoration strategies to address severe degradation. It is often assumed that voluntary forb weeds either compete with or have no effect on native seedlings. In contrast, theory and empirical work suggest a potential facilitative role for such forb weeds. We conducted a manipulative weed exclusion experiment at a semi-arid site in South Texas targeted for large scale forest restoration and subsequently dominated by early successional forb weeds to discern the net effect of these weeds on the growth and survival of target thornscrub tree and shrub seedlings. Overall, weed presence did not significantly affect seedling mortality …

A Python-Based Brain-Computer Interface Package For Neural Data Analysis, Md Hasan Anowar

Theses and Dissertations

Anowar, Md Hasan, A Python-based Brain-Computer Interface Package for Neural Data Analysis. Master of Science (MS), December, 2020, 70 pp., 4 tables, 23 figures, 74 references.

Although a growing amount of research has been dedicated to neural engineering, only a handful of software packages are available for brain signal processing. Popular brain-computer interface packages depend on commercial software products such as MATLAB. Moreover, almost every brain-computer interface software is designed for a specific neuro-biological signal; there is no single Python-based package that supports motor imagery, sleep, and stimulated brain signal analysis. The necessity to introduce a brain-computer interface package that …

Performance And Efficiency Evaluation Of Stormwater Management Lids Using Pcswmm: Numerical Modeling And Sensitivity Analysis, Abdullah Al Fatta

Theses and Dissertations

Implementation of Low Impact Development systems (LIDs) can reduce the detrimental effects of urbanization. In this study, performance of both conventional drainage system and bioretention swales LID green infrastructure was assessed. Personal Computer Storm Water Management Model (PCSWMM) was used to investigate the performance by producing runoff hydrographs. Calibration and validation were conducted with independent storm events to minimize the gap between the simulated and observed outflow. Among different sensitive parameters, saturated hydraulic conductivity, suction head, percent initially saturated, Manning’s N for impervious area, were used in calibration. Each storm event resulted coefficient of determination (R2) values of 0.656 or …

Improving Collaborations Between Empiricists And Modelers To Advance Grassland Community Dynamics In Ecosystem Models, Kevin R. Wilcox, Kimberly J. Komatsu, Meghan L. Avolio, Nathan P. Lemoine

Biological Sciences Faculty Research and Publications

No abstract provided.

Lianas Maintain Insectivorous Bird Abundance And Diversity In A Neotropical Forest, Stefan A. Schnitzer, Nicole L. Michel, Jennifer S. Powers, W. Douglas Robinson

Biological Sciences Faculty Research and Publications

The spatial habitat heterogeneity hypothesis posits that habitat complexity increases the abundance and diversity of species. In tropical forests, lianas add substantial habitat heterogeneity and complexity throughout the vertical forest profile, which may maintain animal abundance and diversity. The effects of lianas on tropical animal communities, however, remain poorly understood. We propose that lianas have a positive effect on animals by enhancing habitat complexity. Lianas may have a particularly strong influence on the forest bird community, providing nesting substrate, protection from predators, and nutrition (food). Understory insectivorous birds, which forage for insects that specialize on lianas, may particularly benefit. Alternatively, …

Governing Wicked Problems, Jb Ruhl, James Salzman

Vanderbilt Law Review

"Wicked problems." It just says it all. Persistent social problems-poverty, food insecurity, climate change, drug addiction, pollution, and the list goes on-seem aptly condemned as wicked. But what makes them wicked, and what are we to do about them?

The concept of wicked problems as something more than a generic description has its origins in the late 1960s. Professor Horst Rittel of the University of California, Berkeley, Architecture Department posed the term in a seminar to describe "that class of social system problems which are ill-formulated, where the information is confusing, where there are many clients and decision makers with …

De- And Re-Constructing Public Governance For Biodiversity Conservation Symposium: Governing Wicked Problems, Alejandro E. Camacho

Vanderbilt Law Review

This Article deconstructs the substantive, procedural, and structural components of public governance in the United States to explain how the existing legal infrastructure lacks the legal adaptive capacity to manage the wickedness of biodiversity loss. That is, particularly in the context of global anthropogenic climate change, the substantive goals and tools of public action, the processes used by governmental institutions to advance such goals and implement such tools, and the structure of allocated authority among public institutions have been devised in ways that make biodiversity loss virtually impossible to tackle meaningfully.

First, the substantive goals of natural resources law are …

Designing Law To Enable Adaptive Governance Of Modern Wicked Problems, Barbara A. Cosens, J.B. Ruhl, Niko Soininen, Lance Gunderson

Vanderbilt Law Review

In the twenty-first century, our planet is facing a period of rapid and fundamental change resulting from human domination so extensive it is expected to be visible in the geologic record. The accelerating rate of change compounds the global social-ecological challenges already deemed “wicked” due to conflicting goals and scientific uncertainty. Understanding how connected natural and human systems respond to change is essential to understanding the governance required to navigate these modern wicked problems. This Article views change through the lens of complexity and resilience theories to inform the challenges of governance in a world dominated by such massive and …

In Vitro Cytotoxicity Study Of Pt Nanoparticles Decorated Tio2 Nanotube Array, Shaymaa R. Baqer, Abdulkareem M. Ali Alsammarraie, Mahasin Alias, Mohammad M. F. Al-Halbosiy, Amaal S. Sadiq

Baghdad Science Journal

Titanium dioxide nanotubes were synthesized by anodizing Ti sheets in the ethylene glycol solution and were covered in Pt nanoparticles onto the surface of TiO2NTs using electrodeposition method from using five derivatives of Mannich base Pt complexes which have been used as precursor of platinum. The mean size, shape, elemental composition of the titanium dioxide nanotubes and platinum deposited on the template were evaluated by different techniques such as field emission scanning electron microscope (FE-SEM), transmission electron microscopy (TEM), X-ray diffraction pattern (XRD), and energy dispersive X-ray (EDX) technique. From all these analyses, the TiO2NTs prepared and Ptnanoparticles deposited on …

A Comparative Analysis Of Rule-Based, Model-Agnostic Methods For Explainable Artificial Intelligence, Giulia Vilone, Lucas Rizzo, Luca Longo

Articles

The ultimate goal of Explainable Artificial Intelligence is to build models that possess both high accuracy and degree of explainability. Understanding the inferences of such models can be seen as a process that discloses the relationships between their input and output. These relationships can be represented as a set of inference rules which are usually not explicit within a model. Scholars have proposed several methods for extracting rules from data-driven machine-learned models. However, limited work exists on their comparison. This study proposes a novel comparative approach to evaluate and compare the rulesets produced by four post-hoc rule extractors by employing …

Behavioral Finance For The Individual Investor, Drake Gens

Senior Honors Theses

The Efficient Market Hypothesis (EMH) has been generally accepted in academia despite its well-researched flaws; by understanding how and when markets deviate from efficiency, investors have an opportunity to not only better understand their investing habits, but also possibly generate higher investment returns. Various market anomalies, such as the Value Effect (De Bondt & Thaler, 1985), the Monday Effect (French, 1980), and the January Effect (De Bondt and Thaler, 1958 & 1987), attest to the fact that markets experience periods of deviation from efficiency. Fiévet and Sornette (2016) finding that markets experience inefficiency during periods of significant volatility is confirmed …

Ensemble Of Convolutional Neural Networks To Improve Animal Audio Classification, Loris Nanni, Yandre M.G. Costa, Rafael L. Aguiar, Rafael B. Mangolin, Sheryl Brahnam, Carlos N. Silla

College of Business

In this work, we present an ensemble for automated audio classification that fuses different types of features extracted from audio files. These features are evaluated, compared, and fused with the goal of producing better classification accuracy than other state-of-the-art approaches without ad hoc parameter optimization. We present an ensemble of classifiers that performs competitively on different types of animal audio datasets using the same set of classifiers and parameter settings. To produce this general-purpose ensemble, we ran a large number of experiments that fine-tuned pretrained convolutional neural networks (CNNs) for different audio classification tasks (bird, bat, and whale audio datasets). …

A Cathedral Not Made By Hands, Vincent Miller

Materials from the University of Dayton Magazine Website

A theologian finds Laudato si’ — everything is connected — in an old-growth forest.

The Design And Validation Of A Qpcr-Based Approach To Cell Quantification Of Fungal-Algal Symbioses, Isabelle Katherine Garlotte

Honors Theses

Symbiotic relationships are extremely common in virtually all species. These relationships between organisms, particularly mutualistic symbioses, can have an incredibly large impact and can greatly affect the evolution of the species involved. Of particular interest is the suspected mutualistic relationship between green algae and fungi that may hold key evidence of how terrestrial plants evolved. In this experiment we established a method of quantifying the cells of each species involved in these symbioses through qPCR analysis to determine if the relationships are truly mutualistic. We developed a system for using DNA copies as a proxy for cell count in co-cultures …

Through The Funnel: A Critical Policy Analysis Of Educational Policy Relating To The School-To-Prison Pipeline., Jesse V. Hall

Special Education ETDs

The purpose of the present study involved analyzing the policies of the Trump administration to determine the ways it impacted the school-to-prison pipeline. The focus of the study included the Departments of Education and Justice. The findings revealed educational policies and deregulatory practices that maintained and intensified the school-to-prison pipeline.

Editor's Choice: Aldo Leopold Conservation Award, Luis A. Ruedas

Biology Faculty Publications and Presentations

Every year since 2003, the American Society of Mammalogists confers during its annual meeting the Aldo Leopold Conservation Award on a highly deserving individual. A consequence of the award for the awardee is the responsibility to write a feature article in the pages of this journal. Aldo Leopold, the namesake of the award, is considered by an overwhelming majority of biologists to be the father of contemporary conservation biology. Born in Burlington, Iowa, Leopold graduated with a Master’s degree from the Yale Forest School, which had been endowed in 1902 by the Pinchot family and was one of the first …

Quantification Of Nitric Oxide Via Single-Walled Carbon Nanotubes, Jakob Meier

Department of Agricultural and Biological Systems Engineering: Dissertations, Theses, and Student Research

Nitric oxide (NO), a free radical present in biological systems, can have many beneficial and detrimental effects on the body. Due to NO’s short half-life, its detection and quantification is difficult. This inability to quantify NO has hindered researchers that are trying to understand NO’s impact in healthy and diseased conditions. Single-walled carbon nanotubes (SWNT), can become selective to various analytes when wrapped in specific single-stranded DNA strands. The presence of the analyte of interest can change the wavelength and/or intensity of the SWNT’s fluorescence, allowing the SWNT to be used as a sensor. One type of SWNT sensor, 6,5 …

The Role Of Liver Sinusoidal Endothelial Cells In Liver Malady Homeostasis, Fatima Cabral

Department of Biochemistry: Dissertations, Theses, and Student Research

Current literature described techniques for the purification of liver cell types through text alone. The techniques described for the isolation of liver sinusoidal endothelial cells as well as hepatocytes described here are modified from a published article in the Journal of Visualized experiments. The video protocol allows for the user to successfully isolate cells as the most difficult parts of the procedure are demonstrated visually. The detection of liver maladies such as the non-alcoholic fatty liver disease, the stage if this disease and differentiation between non-alcoholic and alcoholic fatty liver disease is demonstrated in the development of a unique panel …

Women In Beekeeping: Impacts Of A Beekeeper Educational Program, Bridget Gross

Department of Entomology: Dissertations, Theses, and Student Research

The decline in honey bee populations over the past two decades in the United States is alarming. The management provided by beekeepers to their honey bee colonies influences the survival of the colony. However, there is a lack of information on the experiences of beekeepers, specifically women beekeepers. The Center for Rural Affairs (CFRA) in Nebraska hosted the “Honey Bees on the Farm: Connecting Women Beekeepers and Women Farmers for Environmental and Economic Benefit” program that provided informal, educational events to women beekeepers and landowners. Using a convergent mixed methods design, the first research question examines the impacts of the …

The Effects Of Solution Condition On Virus Filtration Performance, Fnu Namila

Graduate Theses and Dissertations

Virus filtration is an integral part of the downstream purification of mammalian cell culture-derived biotherapeutics to assure the viral safety of the products. Virus filtration membranes remove viruses based on a size-exclusion mechanism. Commercial parvovirus filers possess unique membrane structure and are designed to remove smaller non-enveloped parvoviruses with size 18-26 nm. However, some filters face issues, such as pre-mature fouling, the decline of filtrate flux, and reduction in virus retention. This doctoral dissertation focused on identifying the factors that influence the filtrate flux and the virus retention capability of commercial virus filters. The effects of solution pH and ionic …

Projecting Water Available For Irrigation Use And Identifying Water Supply Stress Under Climate Change Scenarios In Selected U.S. Fruit And Vegetable Production Regions, Andrew Shaw

Graduate Theses and Dissertations

Climate change affects water resources differently across geospatial regions in the United States (U.S). There is a concern of how water availability will be affected by changes in long-term temperature and precipitation patterns, specifically in major production regions for eight fruit and vegetable crops. The effects on surface water available for irrigation use and supply stress in five regions containing 31 Agricultural Statistics Districts (ASDs) were assessed. The Water Supply Stress Index Model was used and modified to project water available for irrigation use across nine climate scenarios driven by historical data, five General Circulation Models, two population scenarios, and …