Digital Commons Network^™

Characterization Of Rhodopseudomonas Palustrus Strains For The Production Of Fixed Nitrogen Fertilizer For Mars, Daniel Kade Derrick

Undergraduate Honors Capstone Projects

The purpose of this project was to characterize the potential of various strains of purple non-sulfur bacteria for the production of fixed nitrogen fertilizers for the manned Mars missions. Six strains of Rhodopseudomonas palustris (CGA009, CGA010, TIE-1, NifA*, and PB23) were all investigated. Through initial growth trial experiments, R. palustris NifA* and PB23 were selected for their engineered nitrogen fixation and rapid growth respectively. Growth curves and ammonium concentrations were collected over time in pilot scale batch photobioreactors (200 mL). Biomass production was then scaled up to benchtop photobioreactors (1.5 L) and then 14 L total volume for both NifA* …

Increasing Knowledge Through Nutrition Education In Adolescents And Children In Klaten, Indonesia, Alexandra Leavitt

All Graduate Plan B and other Reports, Spring 1920 to Spring 2023

Food insecurity continues to plague over 840 million people all over the world. Food insecurity can lead to malnutrition which can result in long-term adverse health effects. Malnutrition has the highest impact on mothers and children living in developing countries as most families are living on less than two U.S. dollars a day. In order to provide enough food, low-income families rely on inexpensive foods, which are often lacking in essential nutrients. More developed countries can provide food aide in the form of commodities or therapeutic foods, but this is only a temporary fix to a continuous problem. The long-term …

Good Grief: An Analysis Of The Character Development Of Tonya In August Wilson’S King Hedley Ii Through The Lens Of “The Five Stages Of Grief”., Kala Ross

Electronic Theses and Dissertations

An actor who is portraying a grieving character can also grieve simultaneously about the character’s given circumstances, which affects the character development and mental health of the actor. This thesis assesses how the character Tonya, in August Wilson’s King Hedley II, engages with grief as an African American woman and offers a process for the actor to engage and disengage with loss. “Good Grief” will unpack Constantin Stanislavski’s system of acting, focusing on the Magic “If” and the given circumstances, while also introducing the “Five Stages of Grief” coined by Elisabeth Kubler-Ross. Through the scholarship of theatre practitioners and psychologists …

Development Of In Vitro Models To Study The Rapid Extraintestinal Dissemination Of Salmonella., Adarsh Gopinath

Electronic Theses and Dissertations

Salmonella appears in the bloodstream of mice in as little as 15 minutes after oral inoculation and establishes persistent colonies in the spleen and liver. While its pathway to blood is undetermined, this phenomenon is dependent on the activity of Salmonella pathogenicity island 2 (SPI-2) coded type III secretion system (T3SS) and CD18+ phagocytes. We hypothesize that dendritic cells associated with the basal face of the gut epithelium, that are naturally migratory and known to sample for luminal antigens directly transport Salmonella to the bloodstream. This process comprises of at least two phases, dissociation and reverse transmigration. We define dissociation …

Modulation Of Host Innate Immune Cells By Yersinia Pestis To Create A Permissive Environment For Replication., Amanda Rose Pulsifer

Electronic Theses and Dissertations

Yersinia pestis has gained widespread infamy due to the historic outbreak during the middle ages, referred to as The Black Death. Infection with Y. pestis typically begins with deposition of Y. pestis into the dermis (bubonic plague) or respiratory tract (pneumonic plague). Tissue resident macrophages are the first innate immune cell encountered by Y. pestis. Macrophages are likely a way for Y. pestis to avoid neutrophils early in infection when the neutrophil neutralizing Type Three Secretion System is not expressed. This work focuses on which Rab host proteins are manipulated by Y. pestis, and how neutrophils are forced to …

The Emerging Oral Pathogen, Filifactor Alocis, Disrupts Neutrophil Functions To Enhance Survival And Dysregulate Inflammation., Irina Miralda Molina

Electronic Theses and Dissertations

Periodontitis is an irreversible, chronic inflammatory disease where pathogenic microbial communities accumulate in the gingival crevice. Advances in culture-independent techniques have facilitated the identification of new bacterial species in periodontal lesions, such as the Gram-positive anaerobe, Filifactor alocis. Neutrophils are a major component of the innate host response, and the outcome of their interaction with F. alocis may be a determinant of oral health status. While neutrophil functions typically protect the host against periodontal disease, oral pathogens have adapted to evade or disarm neutrophil microbicidal functions while promoting mechanisms that drive inflammation, which also provides a source of nutrients …

Role Of Plant Volatile Organic Compounds (Vocs) In Seed Priming And Plant-Herbivore Interactions., Abhinav Kumar Maurya

Electronic Theses and Dissertations

This dissertation explores and expands the existing knowledge on the role of plant volatiles in facilitating seed priming and providing direct defense against herbivore. Although the roles of plant volatiles in priming a plant’s defenses and providing direct defense against pests is well known, information regarding their effects on seed and the fitness of future plants and direct toxicity to herbivores is understudied. This dissertation does a thorough examination of these two understudied aspects of plant volatiles and provides novel insight into the role of plant volatiles in seed priming and direct defense (Chapter I). Seeds in the soil can …

Volatile Organic Compound Exposure And Cardiometabolic Syndrome Risk In A Nationally Representative Cohort., Stacey Lane Konkle

Electronic Theses and Dissertations

BACKGROUND: The relative importance of environmental exposures such as volatile organic compounds (VOCs) is one of the paramount public health priorities of our time, yet is presently unstudied. VOCs are ubiquitous in the environment and have been associated with numerous adverse health effects, including a number of cardiovascular and metabolic effects that are components of Cardiometabolic Syndrome (CMS). OBJECTIVES: To examine the relationship between CMS and individual-level exposures to VOCs, measured as urinary metabolites of VOCs (UM-VOCs), in a nationally representative sample. METHODS: Associations between urinary biomarkers of exposure to 19 parent VOCs and CMS were assessed using the National …

Coparenting And School Success: Perspectives Of Fathers., Shannon M. Mount

Electronic Theses and Dissertations

Historically, measures of coparenting have been reported by mothers. As a result, fathers’ perspectives and experiences have largely been excluded from this body of research. In response, this study relies on fathers own report of coparenting support. This study used a sample of 1255 biological fathers from the Fragile Families and Child Wellbeing Study (FFCWS) to explore fathers’ reports of their coparenting support and how this is associated with relationship status with mother, time spent with child, and several school success variables including: internalizing and externalizing school behaviors and math and reading assessment scores at child age 9. Hypotheses included …

Sulforaphane Protects Against Ethanol-Induced Apoptosis And Teratogenesis Through Epigenetic Modulation Of Anti-Apoptotic Genes., Yihong Li

Electronic Theses and Dissertations

Background. Ethanol-induced excessive apoptosis in neural crest cells (NCCs), a multipotent progenitor cell population, is one of the major mechanisms underlying the pathogenesis of Fetal Alcohol Spectrum Disorders (FASD). However, the molecular mechanisms underlying FASD that results from maternal alcohol exposure during pregnancy are poorly understood. The overall goals of this study are to examine the mechanisms by which ethanol induces apoptosis and malformations in vitro and in vivo, and to develop a nutritional-based approach by using SFN and SFN-rich BSE to prevent FASD through epigenetic modulation. Results. This study demonstrates that ethanol exposure resulted in a significant increase …

Functionalizing Nanoparticles With Cafa Protein To Target Bar Peptide For Oral Delivery Applications., Hetal Desai

Electronic Theses and Dissertations

Background: Porphyromonas gingivalis adherence to Streptococcus gordonii may be important for P. gingivalis colonization in the oral cavity. Nanoparticles encapsulating synthetic peptide BAR (BAR-NPs) inhibit P. gingivalis adherence more potently than free BAR. However, BAR-NPs would exhibit low retention in an open flow environment. Hypothesis: Targeting BAR-NPs to the streptococcal surface using CafA protein will enhance their efficacy. Methods: CafA-modified NPs encapsulating BAR were synthesized using double emulsion approach. Surface binding and retention, and release kinetics of BAR from CafA-modified NPs was assessed. Functional inhibition assays were performed using dual a species biofilm. Results: CafA-modified NPs demonstrated specificity of adhesion, …

Food Insecuity In Historically Disenfranchised Parts Of Jefferson County, Ky., Kaitlyn Smith

Electronic Theses and Dissertations

The objective of this study is to investigate the availability of fresh and nutritious foods in Louisville’s West End communities. This study operates under the assumption that residents in Jefferson County have unequal access to food. This study also addresses the question of how residents and local non-profits feel about strategies that build community through food. This study is significant because across Louisville, more than 120,000 people are living with food insecurity, meaning they don't have reliable access to healthy, affordable food (Dare to Care 2019). Multiple reports that have been commissioned by local non-profits and metro departments in the …

Factors Of Dysphagia In The Overdose Population Post Extubation., Renee Brown

Electronic Theses and Dissertations

Post-extubation dysphagia (PED) has been studied in depth in a variety of populations including elective heart surgeries, trauma, critical illness, traumatic brain injury, and those requiring mechanical ventilation, but has not been researched in the overdose population (OD). This study aims to research factors of PED in the OD population. A retrospective study was conducted and found 24 participants who fit the inclusionary requirements. Correlation statistics were run between age and duration of intubation, age or duration of intubation and gender, and between intubation duration, age, and scores on Penetration-Aspiration Scale (P/A) (thin by cup and thin by straw). The …

Efficient Conditional Inactivation Of Rdh10 Reveals Important Role Of Retinoic Acid During Lung Branching Morphogenesis., Nhut Quang Huy Tran

Electronic Theses and Dissertations

Birth defects are complicated pathological processes. Many birth defects can be traced back to disrupted regulation of molecular signaling pathways during embryogenesis. One of the most important gene regulatory factors during embryo development is retinoic acid (RA). However, studying the specific roles of RA in each organ during embryogenesis in vivo is challenging. The Sandell laboratory has a conditional mutant mouse model that can potentially be used for studying the role of RA during embryogenesis by inducing stage-specific RA deficiency. This model allows retinol dehydrogenase 10 (Rdh10), a gene required for RA production, to be inactivated at a …

Effect Of Dietary Maillard Reaction Products On Insulin Sensitivity, Metabolic Inflammation And Intestinal Inflammation In Mice Fed The Total Western Diet, Siyu Xiao

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Maillard reaction products (MRPs) are generated when proteins or amino acids are heated with reducing sugars. In previous studies, baking whole diet pellets at a high temperature has been the most common way to promote MRP formation. However, baking diets also induces other chemical reactions besides MRP production, for example lipid oxidation. In this study, only casein and sugars were cooked to generate MRPs. Thus, a complementary experiment was conducted to determine how baking diet pellets affects lipid oxidation.

Previous rodent studies showed MRPs either induced weight gain and or impaired glucose tolerance. On the other hand, dietary MRPs were …

Improving Identification Of Pediatric Feeding Dysfunction Among Registered Dietitian Nutritionists, April Litchford

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

All Registered Dietitian Nutritionists (RDN) undergo extensive training to develop the ability to improve dietary intake among individuals of all ages. Treating children (0-18 years of age) is often challenging and requires specialized training. One area that is particularly challenging is identifying children that may not be able to eat appropriately to sustain rapid growth and development. An online survey of RDNs that work specifically with children was conducted to better understand how RDNs are identifying and treating children with feeding problems. From the survey we learned that the methods and procedures used by RDNs for identifying and treating children …

Club Athletes: Dietary Supplement Use And How The Registered Dietitian Can Better Serve This Population, Rachel E. Robbins

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Dietary supplements are a booming business in the United States. Currently over half of Americans have taken a dietary supplement in the last year. Collegiate and professional athletes are even more likely to take a dietary supplement to get a competitive edge over their opponents. The objective of this study was to assess dietary supplement use in club athletes at Utah State University. Club athletes are non-scholarship athletes associated with a university. They often do not the same level of funding or resources that NCAA or scholarship athletes have. A survey was sent to all 401 club athletes at Utah …

The Influence Of Protein Concentration And Homogenization On Moisture Content, Curd Yield, And Fat Retention Of Model Cheese Made From Microfiltered Skim Milk Recombined With Cream, Richard Byron Geslison

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

This project was funded by the Western Dairy Center at Utah State University as part of a multi-pronged approach to improve the current understanding of using concentrated milks in cheese making. Concentrated milk for this study was provided by South Dakota State University.

This study compared the effect of different concentration factors of milk on curd moisture levels, fat content, and cheese curd yields. To see if these results could be improved (i.e. remove more moisture and retain more fat) milk samples were also subjected to limited pressure homogenization (microfluidization) treatments.

It was found in the course of this study …

Effects Of Bovine Maternal Nutrient Restriction On Offspring Microrna And Mrna Expression And Muscle Fiber Type, Nikole E. Ineck

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

For producers in more temperate areas, such as the Intermountain West, poor nutrition during the second trimester of gestation is common due to seasonal changes in forage and nutrient availability. The majority of muscle fibers are formed and adipogenesis is initiated in the second trimester, making it a critical time for skeletal muscle and adipose development in beef cattle. However, the extent to which these changes persist in the offspring postnatally is unknown. In this study, maternal nutrition was restricted during the second trimester in order to analyze the effects of maternal nutrient restriction on offspring skeletal muscle growth. Offspring …

North Dakota Greater Sage-Grouse (Centrocercus Urophasianus) Recovery Project: Using Translocation To Prevent State-Wide Extirpation And Develop Rangewide Protocols, Kade D. Lazenby

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Greater sage-grouse (Centrocurcus urophasianus; hereafter sage-grouse) are the largest grouse species in North America. Sage-grouse occupy 11 western states, extending into North Dakota. North Dakota sage-grouse population is part of the Great Plains Management Zone. Conservation of sage-grouse has been on the forefront of conservation management 1990s. In 2015 the USFWS declared sage-grouse were not warranted for listing based on significant management efforts. Translocations of sage-grouse to prevent populations from extirpation are an example of these efforts.

Translocations have been described as movement and release of animals into a novel environment. There have been more than 7200 sage-grouse …

Effect Of Thermosonication On Viscosity Of Milk Concentrates And Milk Quality And Shelf Life, Vidita Deshpande

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Thermosonication is the application of soundwaves at temperatures ≥50°C. It can be used to improve functional properties, overall quality, and shelf life of dairy products. Milk is concentrated before being spray dried and currently milk concentration is limited by increased viscosity. Decreasing the viscosity of concentrates prior to spray drying could be economically beneficial to dairy processors. Results from this study showed that that thermosonication significantly reduced the viscosity of concentrates such as reconstituted milk protein concentrate (rMPC) and reconstituted skim milk powder (rSMP). This would allow for spray drying of these concentrates at a higher total solids with a …

Ranchers Adapting To Climate Variability In The Upper Colorado River Basin, Utah, Hadia Akbar

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Changes in climate influence agricultural production. This study looks at the impacts of climate variability in the Utah regions of the Upper Colorado River Basin by combining regression techniques with interview data to explore how climate variability affects agricultural production and how the farmers are adapting their practices to these changes. The results show that climate does not have any significant impact on cattle and hay production in the study area on a decadal scale. However, on an annual basis temperature seems to have more impact than precipitation. Among non-climatic variables, commodity prices and their regulations by the government are …

Usu Equine-Assisted Activities And Therapies Facilities Designed Master Plan, Lindsie C. Smith

All Graduate Plan B and other Reports, Spring 1920 to Spring 2023

Equine-Assisted Activities and Therapies (EAAT) is recognized as a therapeutic approach for persons with disabilities. The USU Animal, Dairy, and Veterinary Sciences Department provides EAAT instruction and services; however, they do not have the appropriate facilities to model best-practices in the delivery of these services. This design research entailed the development of a phased masterplan to support the instruction and delivery of equine-assisted activities and therapies in an innovative and accessible environment that supports animal-assisted intervention and natural equine behaviors.

The methodology used to approach the master plan design was derived from Norman K. Booth’s (1990) design process, as described …

Dual Role Of Antimicrobial Peptide Cathelicidin In Alcohol-Associated Liver Disease., Fengyuan Li

Electronic Theses and Dissertations

Patients with alcohol-associated liver disease (ALD) have high morbidity and mortality in its severe forms. One of the major features in different forms of ALD is the altered gut microbiota. Antimicrobial peptides (AMPs) play a crucial role in maintaining gut microbiota homeostasis. LL-37 (known as CRAMP in mouse) is the sole member of the human cathelicidin family and has piqued great research interest for its dual role in modulating microbiota and the immune response in metabolic diseases. Inflammasome activation is an important component of the liver pathophysiology in ALD and requires two signals for its full activation to induce the …

Identifying Protective Factors Against Overweight And Obesity Within The Social Environment Of Women With Low Incomes., Monica M. Adams

Electronic Theses and Dissertations

Over two-thirds of the United States population have overweight or obese (OW/OB) weight statuses due in large part to an obesogenic environment that encourages unhealthful weight related behaviors. The obesogenic environment appears to place a larger burden on women with low incomes as they experience OW/OB disproportionately compared to other groups. Studies seeking to understand the impact of the obesogenic environment on this population have been deficit focused, largely examining environmental risk factors for OW/OB and ignoring protective factors against it. Most women with low incomes do not have an obese weight status and some women who have OW/OB statuses …

Geographic Imaginaries Of Urban Spatial Segregation: A Case Study Of The West End Neighborhoods In Louisville, Kentucky., Amber Dock

Electronic Theses and Dissertations

The objective of this thesis is to translate the framework of geographic imaginaries into an urban context in order to capture a narrative of how residents conceptualize and experience segregation. This framework is rooted in an investigation of local discourses as they exist within a specific social, political, and historical context. Institutionalized segregation and structural racism are the foundations on which the American urban context studied here was built upon. This study employs multiple methods, including contextualizing the study area, analyzing discursive content, and visualizing the results. The results of these analyses included empirically connecting concentrations of protected classes to …

Attitudes Of Health Profession Faculty Involved In A Simulation-Based Interprofessional Education Experience, Shannon Ashe

Doctoral Dissertations and Projects

Interprofessional education and collaborative practice are the current standards of the global healthcare field. In order to better understand the barriers to completing simulation-based interprofessional education (Sim-IPE), faculty attitudes and perceptions need to be identified and addressed. In the current study, the researcher sought to identify the attitudes of health profession faculty at a private university within a college of health sciences that includes over 20 academic programs, exclusive of medicine, in relation to a planned large-scale Sim-IPE in the acute care setting. Utilizing both a correlational and repeated measures approach, the researcher explored a previously planned interprofessional experience that …

Exploring The Lived Experiences And Coping Strategies Of Parents Of Children With Profound Intellectual And Multiple Disabilities, Anne Glaeser Hubach

Doctoral Dissertations and Projects

The purpose of this transcendental phenomenological study was to describe the lived experiences and coping strategies of parents of children with profound intellectual and multiple disabilities (PIMD) in Central Virginia. Research questions used to frame this study are: How do parents of children with PIMD describe their experiences as parents of children with PIMD? How do the parents of children with PIMD describe the impact of their experiences on their well-being? How do the parents of children with PIMD describe their understanding of and perspectives regarding their children’s disabilities? How do parents of children with PIMD describe the impact of …

Physiological And Molecular Outcomes Of Chronic Post-Weaning Folate Deficiency, Abigail Joan Lawton

Doctoral Dissertations and Projects

Folate is an essential B vitamin that serves as a primary one-carbon carrier in a number of essential cellular reactions; among these reactions are synthesis of purines, thymidylate, and methionine. As a result of these synthesis reactions, folate plays a key role in regulating nucleic acid metabolism, amino acid metabolism, maintenance of DNA stability, and production of S-adenosylmethionine for methylation of nucleic acids, neurotransmitters, phospholipids, histones, and other proteins. As such, folate deficiency is associated with a number of adverse outcomes including various cancers, cardiovascular disease, and cognitive defects. Despite mandatory folate fortification in grains in many developed countries, a …

Tobacco Enhances Bacterial-Induced Periodontal Bone Loss In Mice., Mina Iskander

Electronic Theses and Dissertations

Background: Tobacco smoking is the leading environmental risk factor for periodontal diseases. Delineation of the mechanisms underlying tobacco-induced or exacerbated periodontitis is hampered by the lack of an appropriate and reliable animal model. Hypothesis: We hypothesized that Porphyromonas-gingivalis-infected, cigarette smoke-exposed mice would represent reproducible models of acute (ligature model) and chronic (oral gavage model) tobacco-enhanced periodontitis that reflect multiple aspects of the disease noted in human smokers. Methods: In a chronic oral gavage disease model, Balb/c mice (6-8 weeks, 4 groups of n = 6 per group) were exposed to smoke produced by a Teague-10 smoking machine from 1R6F research …