Digital Commons Network^™

Developing A Maceration Method For Isolation Of Dna From Cancellous Bones, Bryan Bernal

Student Theses

Prior to isolating DNA, it is necessary to remove any biological materials that may be present on commingled remains. Manual maceration of the outer surface is time-consuming. This study will address this issue by developing a simple method for processing bone samples prior to DNA isolation. A liquid-based technique was applied to macerate bone fragments by incubating bone fragments in an enzyme solution, removing potential contaminants, followed by a bone tissue disruption. Swine (Sus scrofa) bones were used to simulate human bones. Microscopic analysis suggested this method was effective for surface material removal. The methods effect on the …

Indian Ocean Territories Price Index 2019, Department Of Primary Industries And Regional Development, Western Australia

Statistics

The Department of Infrastructure, Transport, Regional Development and Communications has responsibility for the Indian Ocean Territories, with many services provided by service delivery arrangements with the Western Australian Government. In 2011, the then-Department of Regional Development was approached by the Commonwealth government to develop and conduct a cost of living index for Christmas and Cocos (Keeling) Islands, known collectively as the Indian Ocean Territories. The result of this project was the publication in October 2012 of the first Indian Ocean Territories Price Index

‘Remember The Deeds Of The Lord:’ Collective Memory’S Role In A Reasonable Faith, Baily Ray Mcdaniel

Masters Theses

Faith has long been regarded by secular scholars as unreasonable and unjustified. One of the largest issues that Christians face today is working against the notion that faith is blind. Best-selling author Richard Dawkins states, “religious faith is an especially potent silencer of rational calculation, which usually seems to trump all others.” The irrationality of faith is echoed consistently by other scholars including Bertrand Russell. As Kelly James Clark points out, “Bertrand Russell was once asked, if he were to come before God, what he would say to God. Russell replied, ‘Not enough evidence God, not enough evidence.’” The claim …

First-Principles Studies On The Piezoelectricity And Ferroelectricity Of Knn Related Materials, Yuting Peng

Physics Dissertations - Archive

Nowadays, with the increasing environmental concerns, K1-xNaxNbO3 (KNN)-based materials have gained enormous interest and attention as one of the most promising lead-free piezoelectric materials because of their excellent piezoelectric performance and high Curie temperatures. There are lots of experimental efforts focusing on improving the piezoelectricity of KNN ceramics. However, it is lack of theoretical explanations for the intrinsic physical mechanism. In this dissertation, density functional theory (DFT) based first-principles calculations implemented by the Vienna ab initio simulation package (VASP) are performed to investigate the electronic structure, ferroelectric and piezoelectric properties of KNN related materials, including the pure KNbO3 (KN) and …

General Catalog 2020-2021, Utah State University

University Catalogs

This catalog contains information on the Academic Departments and Programs as well as Course Descriptions for the above school year at Utah State University. To avoid large file downloads, the Academic Departments and Programs information is located in this file. The Course Description information is located in the secondary file.

Development, Expansion And Role Of Myeloid-Derived Suppressor Cells In Post-Sepsis Immune Suppression, Tuqa Alkhateeb

Electronic Theses and Dissertations

Myeloid-derived suppressor cells (MDSCs) numbers increase significantly in sepsis and are associated with high mortality rates. These myeloid cell precursors promote immunosuppression, especially in the late (post sepsis) stage. However, the mechanisms that underlie MDSC expansion and programming are not completely understood. To investigate these mechanisms, we used a cecal-ligation and puncture (CLP) mouse model of polymicrobial sepsis that progresses from an early/acute proinflammatory phase to a late/chronic immunosuppressive phase. Previous studies in our laboratory showed that microRNA (miR)-21 and miR-181b elevate levels of the transcription factor nuclear factor 1 (NFI-A) that promotes MDSC expansion. We report here that miR-21 …

Optimizing Species Selection For Forest Restoration In The Lower Rio Grande Valley, Faeqa Mohsin

Theses and Dissertations

Over the past four decades, the Lower Rio Grande Valley has been reforested with over 3.3 million native thornscrub plants in old agricultural land through transplanting native species. However, assessments of LRGV forest restoration efforts are scarce, and to address the knowledge gap, this study examined species-specific responses to restoration interventions (RIs) aimed at minimizing seedling mortality for optimizing species selection. A critical period for forest restoration in old croplands is the seedling stage, where high mortality occurs due to the interactive effects of abiotic stress, compromised soil nutrient status from intensive agriculture, and mammalian herbivory. So, we evaluated seedling …

The Long-Term Persistence And Tissue Tropism Of Brazilian Zika Virus In Monodelphis Domestica, Marisol Morales

Theses and Dissertations

Zika virus (ZIKV) rose as a major public health concern due to the congenital abnormalities of infants born to infected mothers during the 2015 Latin American outbreak. While animal models such as transgenic mice and nonhuman primates are used to study ZIKV pathogenesis, they display various limitations. Additionally, the persistence and tissue distribution of long-term ZIKV infection remains unknown. We describe the laboratory opossum, Monodelphis domestica, as a potential new model for ZIKV pathogenesis research to address these issues. We utilized immunohistochemistry, RT-PCR, and ELISA to show that: infant animals inoculated intracerebrally were susceptible to infection, ZIKV infection persisted …

Analysis Of Fantasy Fiction Series Of Sarah J. Maas: A Court Of Thorns And Roses, Raelynn D. Peña

Theses and Dissertations

This thesis offers a feminist interpretation of A Court of Thorns and Roses, a series by Sarah J. Maas. The fantasy fiction series began publication in 2015 and released its companion book in 2018. Protagonist Feyre navigates values about femininity and masculinity, breaking standards, as she develops throughout the series to change the fae and human worlds. Feyre stands up to inequality and helps others, both human and fae, to make peace instead of war. This analysis uncovers the gender roles, literary elements, and fairy tale influences on the series A Court of Thorns and Roses. Prominent symbolism involves masks, …

Aquatic Stage Mosquito Biocontrol: Effects Of Entomopathogenic Fungi Blastospores And Conidia On Aedes Aegypti Pupae, Ricardo Alberto Ramirez Garcia Rojas

Theses and Dissertations

Fungal entomopathogens present an opportunity to produce mosquito controls on-site, from remote locations to developing communities. Locally produced biopesticides have the potential to empower communities to develop internal disease vector control initiatives to protect local public health. We tested the effectiveness of the conidia solution and blastospore solution of Metarhizium anisopliae E9, Metarhizium anisopliae E1037, Isaria fumosorosea A97, and Isaria fumosorosea 9901 in controlling Aedes aegypti pupae. We observed that entomopathogenic fungi liquid blastospore solution induce mortality in Aedes aegypti pupae at a significantly greater rate than conidia solution and control treatment.

However, further analysis of testing methods used in …

Effects Of Seasonal Variations And Heat Exposure On Antioxidant Expression And Redox Status In The American Oyster, Crassostrea Virginica: Field And Laboratory Studies, Md Sadequr Rahman

Theses and Dissertations

Increasing atmospheric temperatures influence physiological functions in aquatic organisms. In this study, I observed the effects of seasonal (field study) and laboratory heat exposure (16, 22, 26, and 30°C for 1-week) on prooxidant-antioxidant homeostasis in gills and digestive glands of American oyster. Histological analysis showed an increase in mucus secretion with elevated temperatures in both organs. Immunohistochemical analysis showed a significant increase of heat shock protein-70, dinitrophenyl, and 3-nitrotyrosine protein expressions with higher seasonal and laboratory temperatures indicating heat-induced oxidative and nitrosative stress. This was concomitant with gradual rise in cellular apoptosis. Interestingly, catalase and superoxide dismutase expressions also increased …

The Wind Still Blows: Poems, Sarah Alicia Solis

Theses and Dissertations

The Wind Still Blows: Poems is a collection of autobiographical poems and lyric essays that explore my journey with faith. This collection reveals the life of a young woman learning to meld Chicana feminist ideas and Christian belief. With regards to this introduction, the first-person “I” will be used to denote specific craft choices and critical frameworks that I, the writer, used within the collection, and third-person references to “the speaker” refer to the constructed narrator described in the poems. In this collection, the speaker offers up a history with anxiety disorders, fraught relationships with male figures, developing into an …

Exploring Plant-Plant Interactions And Nutrient Manipulation As Strategies For Thorn Scrub And Thorn Forest Restoration, Edgar D. Vasquez

Theses and Dissertations

Restoration of thorn scrub and thorn forest communities in South Texas is hindered by harsh environmental conditions and hypercompetitive invasive grasses. The success of thorn scrub/thorn forest restoration efforts depends on increasing seedling survival and growth by reducing stress after transplantation. Four experiments evaluated initial effects of plant-plant interactions and nutrient manipulation on thorn forest restoration. High density planting along with the use of nurse plants look promising; survival was generally higher under these conditions and growth was unaffected or in the case of high-density planting, promoted. Nutrient sequestration by sorghum had little effect on seedling performance and cover of …

The Application Of Advanced Technologies For Agriculture And Rangeland Management, Matthew D. Kutugata

Theses and Dissertations

This project demonstrates two applications of remote sensing in agricultural and rangeland environments. In the first, an unmanned aerial system (UAS) equipped with a multi-spectral sensor was used to estimate canopy cover across four different cover crop trials at four time periods. In the second, a local database of stationary camera trap images of wildlife was used to train a convolutional neural network to automatically catalogue images by identifying the animal in those images. Both projects aimed to provide an example of how remote sensing platforms and machine learning techniques can facilitate the rapid collection and processing of large-scale field …

Artificial Night Lighting And Anthropogenic Noise Alter Animal Activity, Body Condition, Species Richness, And Community Structure, Joshua Willems

Master's Theses

Sensory pollution from artificial night-lighting and anthropogenic noise have increased at a dramatic rate over the last several decades. Alterations to the sensory environment have been found to affect wildlife in a wide variety of ways including behavioral changes, physiological responses, changes in species interactions, and altered community structure. Increased levels of light and noise pollution can originate from many sources including roads, energy development and infrastructure, and urbanization. Even remote or protected areas are not immune to the effects of increased sensory disturbances with 63 percent of protected areas within the United States found to have been exposed to …

Science Fiction And The Law: A New Wigmorian Bibliography, Jorge L. Contreras

Utah Law Faculty Scholarship

In 1908, Dean John Henry Wigmore compiled a list of novels that no lawyer could “afford to ignore”. Wigmore’s list, taken up by Professor Richard Weisberg in the 1970s, catalogs one hundred novels, stories and dramatic works from Antigone to The Merchant of Venice to Native Son, each of which portrays or offers insight into the legal system or the practice of law. Weisberg’s updated list also includes a compilation of critical studies in the then-emerging law and literature movement. This article undertakes a similar bibliographic exercise with respect to law and the literature of science fiction. While science fiction, …

Amendment Clauses In Easements: Ensuring Protection In Perpetuity, Nancy Mclaughlin

Utah Law Faculty Scholarship

Internal Revenue Code § 170(h)(5)(A) requires that the conservation purpose of a deductible conservation easement be “protected in perpetuity.” This article explains how the protected-in-perpetuity requirement should limit the parties’ ability to reserve the right to make post-donation changes to the terms of a deductible easement.

Potential Toxicity Of Silver Nanoparticles To Microbial Communities And Macroinvertebrates, Joseph Kusi

Electronic Theses and Dissertations

Silver nanoparticles (AgNPs) are the most common nanomaterials incorporated in commercial products due to their antimicrobial activity. Recently, AgNPs were detected in surface waters suggesting the potential for bioavailability in the aquatic receptor organisms. This dissertation research attempts to understand the potential toxicity of AgNPs on water quality indicators, focusing on the microbial community and amphipods. This study evaluated whether: (1) the antimicrobial properties of AgNPs pose potential risks to microbial communities in pathogen impaired streams; (2) AgNPs can cause a shift in functional diversity and metabolic fingerprinting of microbial communities; (3) survival and growth of Hyalella azteca (amphipods) could …

Paleoenvironmental Reconstruction Of Quaternary Saltville, Virginia, Using Ostracode Autecology, Austin Gause

Electronic Theses and Dissertations

The Saltville valley in southwestern Virginia is home to Quaternary localities containing paleontological and archaeological remains. Historically the valley has been mined for salt and the small lakes, ponds and springs along the valley floor have a brackish signature. A preliminary report on the site’s ostracode fauna suggested that the site’s water was not always saline. This study analyzed modern and Quaternary ostracodes to understand the valley’s hydrologic and chemical evolution. Sediments contained primarily freshwater species, including the environmentally sensitive Candona crogmaniana. The presence of Pelocypris tuberculatum and a new Fabaeformiscandona species throughout a vertical section spanning the latest Pleistocene …

Determining If Classroom Pets As Part Of An Empathy-Based Intervention Affect Public Elementary School Students’ Empathy, Randa Dunlap

Electronic Theses and Dissertations

The purpose of this mixed methods study was to determine if consistent exposure to classroom pets in public elementary school classrooms affected children’s empathy. The sample included 44 students (females = 22; males = 22; mean age = 8.3 years) from four third-grade classrooms. A pre-test, post-test design was used to measure students’ empathy levels, and percentage changes in empathy scores were recorded for all groups. The sample was a convenience sample, and random assignment to treatment groups was not possible. Treatment included using classroom pet fish along with empathy-based lessons. Of the four participating classrooms, one classroom was the …

Energy Harvesting Using Flextensional Piezoelectric Energy Harvesters In Resonance And Off-Resonance Modes, Mohamed A. Shabara

Mechanical & Aerospace Engineering Theses & Dissertations

Energy harvesting technologies are integrated into various modern devices and systems. These systems include Artificial Intelligence (AI) systems, Internet of Things (IoT), various types of energy harvesters are integrated in many engineering applications such as automotive, aerospace and ocean engineering. In order to develop a fully functioning stand-alone system, it is essential to integrate it with a built in power source such as a battery or a power generator. Also, in many situations, city power sources might not be available. Therefore, reliable, renewable and sustainable local power generators are desired. Piezoelectric energy harvesting (PEH) technologies, which are piezoelectric material-based devices, …

Reflections Of The Angiotensin Receptor Blocker Recall By The Fda And Repercussions On Healthcare, Michael Gillette, Addison Taylor, Djenita Butulija, Himabindu Kadiyala, Hani Jneid

Faculty, Staff and Students Publications

PURPOSE: Beginning in July of 2018, the FDA issued a voluntary recall regarding the presence of a contaminant found in the manufacturing of valsartan. What would ensue has become a largely unprecedented sequence of alarming events since the FDA began reporting public recalls, withdrawals and safety alerts on their website in 2016. Since then, the United States has been significantly impacted by drug recalls affecting angiotensin receptor blockers. This report arms clinicians with additional guidance and provides a framework for responding appropriately to future similar incidents and includes an overview of the angiotensin receptor blockers, and their effects and safety …

Group Treatment Effectiveness For Substance Use Disorders: Abstinence Vs. Harm Reduction, Jill D. Parramore

Counseling & Human Services Theses & Dissertations

The purpose of this systematic review and meta-analysis was to compare group treatment effectiveness for substance use disorders within the U.S. across treatment philosophies as it relates to the primary research question, Is there a significant difference of group treatment effectiveness between Abstinence and Harm Reduction treatment philosophies? It was hypothesized that group treatment will remain an effective intervention to treat substance use disorders between treatment philosophies and that no significant differences exist between-group comparisons. The aim of this study is to provide evidence of treatment effectiveness that will ultimately improve treatment outcomes for substance use disorders, provide guidance for …

The Effects Of Elevation On Foraging Behavior Of Bats In Southern Appalachia, Victoria Long

Electronic Theses and Dissertations

There are limited studies on bat activity in higher elevations in the Appalachian region. Levels of bat activity were compared in south central Appalachia at low (< 914.4 m) and high (> 1, 524 m) elevations in open, forest edge, and riparian habitats. Additionally, habitat suitability was modeled for a common species, big brown bat (Eptesicus fuscus). The study started May 27^th 2019, and sites were monitored biweekly until October 2^nd 2019. Six species and one genus were recorded during the study. Species from the Myotis genus were grouped together because of similar call characteristics. Results show that species were significantly more …

August 2020 News Releases, University Of Montana--Missoula. Office Of University Relations

University of Montana News Releases, 1928, 1956-present

No abstract provided.

Exoenzyme Y Induces Extracellular Active Caspase-7 Accumulation Independent From Apoptosis: Modulation Of Transmissible Cytotoxicity, Phoibe Renema, Natalya Kozhukhar, Viktoriya Pastukh, Domenico Spadafora, Sunita Subedi Paudel, Dhananjay T. Tambe, Mikhail Alexeyev, Dara W. Frank, Troy Stevens

University Faculty and Staff Publications

Caspase-3 and -7 are executioner caspases whose enzymatic activity is necessary to complete apoptotic cell death. Here, we questioned whether endothelial cell infection leads to caspase-3/7-mediated cell death. Pulmonary microvascular endothelial cells (PMVECs) were infected with Pseudomonas aeruginosa (PA103). PA103 caused cell swelling with a granular appearance, paralleled by intracellular caspase-3/7 activation and cell death. In contrast, PMVEC infection with ExoY⁺ (PA103 ΔexoUexoT::Tc pUCPexoY) caused cell rounding, but it did not activate intracellular caspase-3/7 and it did not cause cell death. However, ExoY⁺ led to a time-dependent accumulation of active caspase-7, but not caspase-3, …

Development Of An Endothelial Cell-Restricted Transgenic Reporter Rat: A Resource For Physiological Studies Of Vascular Biology, Mikhail Alexeyev, Aron M. Geurts, Naga S. Annamdevula, C. Michael Francis, Silas Josiah Leavesley, Thomas C. Rich, Mark S. Taylor, Mike T. Lin, Ron Balczon, Jennifer Michelle Knighten, Diego F. Alvarez, Troy Stevens

University Faculty and Staff Publications

Here, we report the generation of a Cre-recombinase (iCre) transgenic rat, where iCre is driven using a vascular endothelial-cadherin (CDH5) promoter. The CDH5 promoter was cloned from rat pulmonary microvascular endothelial cells and demonstrated ~60% similarity to the murine counterpart. The cloned rat promoter was 2,508 bp, it extended 79 bp beyond the transcription start site, and it was 22,923 bp upstream of the translation start site. The novel promoter was cloned upstream of codon-optimized iCre and subcloned into a Sleeping Beauty transposon vector for transpositional transgenesis in Sprague-Dawley rats. Transgenic founders were generated and selected for iCre expression. Crossing …

Eece 702 Social Foundations Of Early Childhood Education, Andrew Aprile

Open Educational Resources

This course is an introduction to the social, historical, and philosophical foundations of early childhood education in the United States. Through critical analysis of required reading, class discussion, and writing, we will explore how the dynamics of schooling relate to larger social, cultural, economic, political and historical forces. Using a sociocultural lens, this course will investigate the ways that social class, race, gender, family, community, language, ability, ethnicity, immigration, and sexuality intersect and impact schools, student outcomes, and policies surrounding early childhood and childhood education. This course places emphasis on the separate and combined effects of race and class within …

A Study Of The United States Influence On German Eugenics., Cameron Williams

Electronic Theses and Dissertations

This thesis is a study of the influence and effects that the United States had upon Germany from the rise of eugenics to its fall following the end of World War II. There are three stages to this study. First, I examine the rise of eugenics in the United States from its inception to the end of World War I and the influence it had upon Germany. Then I examine the interwar era along with the popularization of eugenics within both countries before concluding with the Second World War and post war era.

My thesis focuses on both the active …

Roles Of Endothelial Cell Heat Shock Protein A12b And Β-Glucan, A Reagent For Trained Immunity In The Regulation Of Inflammation In Sepsis, Fei Tu

Electronic Theses and Dissertations

Sepsis is dysregulated host immune response to infection causing life-threatening organ dysfunction. Endothelial cell dysfunction and uncontrolled inflammatory responses are two contributors for sepsis-induced mortality. The crosstalk between endothelial and immune cells plays a critical role in the pathophysiology of sepsis. Therefore, understanding the mechanism of interaction between endothelial and immune cells will provide novel information to develop therapeutic strategies for sepsis.

Pathogen associated moleculear patterns (PAMPs) and/or damage associated molecular patterns (DAMPs) produced during sepsis, activate endothelial cells to increase the expression of adhesion molecules, attracting immune cell infiltration into the tissues. Uncontrolled inflammatory responses during the early phase …