Digital Commons Network^™

Abundance And Distribution Of The Non-Native Apple Snail, Pomacea Maculata, In A Restored Wetland Of An Urban Lake, Lynsee Taylor Crichton

USF St. Petersburg campus Master's Theses (Graduate)

The Pomacea maculata, Perry, 1810 or island apple snail is an invasive species to Florida freshwater systems, has become widespread throughout the state. Although P. maculata in the United States are largely limited to ponds and lakes, it can also occur in other areas such as rivers, irrigated croplands, estuaries, and drainage and irrigation ditches. P. maculata is known for its distinctive reproduction patterns and clutch characteristics. There has been a concern that P. maculata has led to extirpations of the native Florida apple snail P. paludosa. In this study, I examined the abundance and distribution of clutches produced by …

Trophic Transfer, Bioaccumulation And Transcriptomic Effects Of Permethrin In Inland Silversides, Menidia Beryllina, Under Future Climate Scenarios, Andrew Patrick Derby, Neil W. Fuller, Kara E. Huff Hartz, Amelie Segarra, Richard E. Connon, Susanne M. Brander, Michael J. Lydy

Data Sets

This excel workbook contains data from a study of trophic transfer of radiolabeled (C-14) permethrin from pyrethroid-resistant Hyalella azteca to a known predator, the Inland silverside Menidia beryllina. Fish were fed 15 dosed (~ 1 ng/animal) or control H. azteca for 14 days at three temperatures, 18, 23 and 28°C, and three salinities (6, 13 and 20 PSU) to simulate changes anticipated under global climate change (GCC). Following feeding, fish were analyzed for total permethrin concentrations, as well as percent parent compound as a a measure of biotransformation, were measured in both H. azteca and M. beryllina.

Discussion On 'A.R. Wallace In The Light Of Historical Method' By John Van Whye, Charles H. Smith, George Beccaloni, James D. Williams, Derek Partridge

Faculty/Staff Personal Papers

The recent article by historian John van Wyhe purports to identify seventeen ‘myths’ concerning the life and work of naturalist Alfred Russel Wallace. Here we briefly describe what we feel is wrong with them, and refer to published literature that extend these arguments. Our objections do not extend to the ‘historical method’ van Wyhe adopts, but instead to the way he has ignored the criticisms of peers to the extent of not even acknowledging their scholarly articles.

Alfred Russel Wallace Notes 10: The Impact Of A.R. Wallace's Sarawak Law Paper Resurrected, Derek Patridge, Charles H. Smith Editor

Faculty/Staff Personal Papers

Six assertions that relate to the impact of Wallace’s Sarawak Law paper1 on the development of evolution theory have been proposed and analyzed by John van Wyhe.2 He concluded that they were all erroneous. The analysis presented a valid criticism of some casual and over-confident pronouncements with respect to interpretations of history. More significantly, it is a misguided attempt to expose “original historical meanings,” and thereby dismiss all other interpretations as necessarily incorrect. A re-analysis reveals that, contrary to van Wyhe’s analysis, much of the conventional wisdom is plausibly correct, and it remains the case that “the past is a …

Shooting Fish, Michael L. Smith

Kentucky Journal of Equine, Agriculture, & Natural Resources Law

No abstract provided.

Heat, Health And Hatchlings: Associations Of In Situ Nest Temperatures With Morphological And Physiological Characteristics Of Loggerhead Sea Turtle Hatchlings From Florida, Kelsey Alexandra Fleming, Justin R. Perrault, Nicole I. Stacy, Christina M. Coppenrath, Alison M. Gainsbury

USF St. Petersburg Campus Faculty Publications

Incubation temperatures, in addition to an embryo’s genetic makeup, are critical in many aspects of adequate sea turtle embryonic development. The effects of high and low incubation temperatures on hatchling quality have been previously examined; however, many of these studies were conducted on relocated or laboratory-reared nests, which do not accurately reflect natural nest temperature fluctuations. To observe the impacts of varying in situ incubation temperatures on loggerhead sea turtle (Caretta caretta) hatchling morphology, various health variables and locomotor performance, temperature data loggers were deployed in 15 loggerhead nests on Juno Beach, Florida, between May and July 2018. Over the …

Unstructured Biology Of Proteins From Ubiquitin-Proteasome System: Roles In Cancer And Neurodegenerative Diseases, Kundlik Gadhave, Prateek Kumar, Shivani K. Kapuganti, Vladimir N. Uversky, Rajanish Giri

Molecular Medicine Faculty Publications

The 26S proteasome is a large (~2.5 MDa) protein complex consisting of at least 33 different subunits and many other components, which form the ubiquitin proteasomal system (UPS), an ATP-dependent protein degradation system in the cell. UPS serves as an essential component of the cellular protein surveillance machinery, and its dysfunction leads to cancer, neurodegenerative and immunological disorders. Importantly, the functions and regulations of proteins are governed by the combination of ordered regions, intrinsically disordered protein regions (IDPRs) and molecular recognition features (MoRFs). The structure–function relationships of UPS components have not been identified completely; therefore, in this study, we have …

Yersinia Outer Membrane Vesicles As Potential Vaccine Candidates In Protecting Against Plague, Andrey A. Byvalov, Ilya V. Konyshev, Vladimir N. Uversky, Svetlana V. Dentovskaya, Andrey P. Anisimov

Molecular Medicine Faculty Publications

Despite the relatively low incidence of plague, its etiological agent, Yersinia pestis, is an exceptional epidemic danger due to the high infectivity and mortality of this infectious disease. Reports on the isolation of drug-resistant Y. pestis strains indicate the advisability of using asymmetric responses, such as phage therapy and vaccine prophylaxis in the fight against this problem. The current relatively effective live plague vaccine is not approved for use in most countries because of its ability to cause heavy local and system reactions and even a generalized infectious process in people with a repressed immune status or metabolic disorders, as …

Yersinia Pestis Plasminogen Activator, Florent Sebbane, Vladimir N. Uversky, Andrey P. Anisimov

Molecular Medicine Faculty Publications

The Gram-negative bacterium Yersinia pestis causes plague, a fatal flea-borne anthropozoonosis, which can progress to aerosol-transmitted pneumonia. Y. pestis overcomes the innate immunity of its host thanks to many pathogenicity factors, including plasminogen activator, Pla. This factor is a broad-spectrum outer membrane protease also acting as adhesin and invasin. Y. pestis uses Pla adhesion and proteolytic capacity to manipulate the fibrinolytic cascade and immune system to produce bacteremia necessary for pathogen transmission via fleabite or aerosols. Because of microevolution, Y. pestis invasiveness has increased significantly after a single amino-acid substitution (I259T) in Pla of one of the oldest Y. pestis …

Linear Relationships Between Partition Coefficients Of Different Organic Compounds And Proteins In Aqueous Two-Phase Systems Of Various Polymer And Ionic Compositions, Nuno R. Da Silva, Luisa A. Ferreira, Pedro P. Madeira, José A. Teixeira, Vladimir N. Uversky, Boris Y. Zaslavsky

Molecular Medicine Faculty Publications

Analysis of the partition coefficients of small organic compounds and proteins in different aqueous two-phase systems under widely varied ionic compositions shows that logarithms of partition coefficients for any three compounds or proteins or two organic compounds and one protein are linearly interrelated, although for protein(s) there are ionic compositions when the linear fit does not hold. It is suggested that the established interrelationships are due to cooperativity of different types of solute–solvent interactions in aqueous media. This assumption is confirmed by analysis of distribution coefficients of various drugs in octanol-buffer systems with varied ionic compositions of the buffer. Analysis …

The Pathophysiological Significance Of Fibulin-3, Imogen Livingstone, Vladimir N. Uversky, Dominic Furniss, Akira Wiberg

Molecular Medicine Faculty Publications

Fibulin-3 (also known as EGF-containing fibulin extracellular matrix protein 1 (EFEMP1)) is a secreted extracellular matrix glycoprotein, encoded by the EFEMP1 gene that belongs to the eight-membered fibulin protein family. It has emerged as a functionally unique member of this family, with a diverse array of pathophysiological associations predominantly centered on its role as a modulator of extracellular matrix (ECM) biology. Fibulin-3 is widely expressed in the human body, especially in elastic-fibre-rich tissues and ocular structures, and interacts with enzymatic ECM regulators, including tissue inhibitor of metalloproteinase-3 (TIMP-3). A point mutation in EFEMP1 causes an inherited early-onset form of macular …

Mmtr/Dmap1 Sets The Stage For Early Lineage Commitment Of Embryonic Stem Cells By Crosstalk With Pcg Proteins, Young Jin Lee, Seung Han Son, Chang Su Lim, Si Woo Lee, Sangwoon Lee, Jinseon Jeon, Dae Hyun Ha, Na Rae Jung, Su Youne Han, Byung-Rok Do, Insung Na, Vladimir N. Uversky, Chul Geun Kim

Molecular Medicine Faculty Publications

Chromatin remodeling, including histone modification, chromatin (un)folding, and nucleosome remodeling, is a significant transcriptional regulation mechanism. By these epigenetic modifications, transcription factors and their regulators are recruited to the promoters of target genes, and thus gene expression is controlled through either transcriptional activation or repression. The Mat1-mediated transcriptional repressor (MMTR)/DNA methyltransferase 1 (DNMT1)-associated protein (Dmap1) is a transcription corepressor involved in chromatin remodeling, cell cycle regulation, DNA double-strand break repair, and tumor suppression. The Tip60-p400 complex proteins, including MMTR/Dmap1, interact with the oncogene Myc in embryonic stem cells (ESCs). These proteins interplay with the stem cell-related proteome networks and regulate …

Collagen Xii Is A Regulator Of Corneal Stroma Structure And Function, Mei Sun, Nabeel Zafrullah, Floriane Devaux, Chinda Hemmavanh, Sheila Adams, Noel M. Ziebarth, Manuel Koch, David E. Birk, Edgar M. Espana

Molecular Pharmacology & Physiology Faculty Publications

Purpose: The aim of this study was to determine the roles of collagen XII in the regulation of stromal hierarchical organization, keratocyte organization, and corneal mechanics.

Methods: The temporal and spatial expression of collagen XII at postnatal days 4, 10, 30, 90, and 150 were evaluated in wild-type (WT) mice. The role of collagen XII in hierarchical organization was analyzed by measuring fibril diameter and density, as well as stromal lamellar structure, within ultrastructural micrographs obtained from WT and collagen XII-deficient mice (Col12a1–/–). Keratocyte morphology and networks were assessed using actin staining with phalloidin and in vivo confocal …

Effects Of An Exogenous Ketone Supplement On Five‐Kilometer Running Performance, Philip J. Prins, Andrew P. Koutnik, Dominic P. D’Agostino, Christopher Q. Rogers, Jacob F. Seibert, Jillian A. Breckenridge, Daniel S. Jackson, Edward J. Ryan, Jeffrey D. Buxton, Dana L. Ault

Molecular Pharmacology & Physiology Faculty Publications

Numerous oral ketone supplements are marketed with the claim that they will rapidly induce ketosis and improve exercise performance. The purpose of this study was to assess exercise performance time and related physiological, metabolic and perceptual responses of recreational endurance runners after ingestion of a commercially available oral ketone supplement. Recreational endurance runners (n = 10; age: 20.8 ± 1.0 years; body mass: 68.9 ± 5.6 kg; height: 175.6 ± 4.9 cm) participated in a double-blind, crossover, repeated-measures study where they were randomized to 300 mg.kg-1 body weight of an oral β-hydroxybutyrate-salt + Medium Chain Triglyceride (βHB-salt+MCT) ketone supplement or …

Ketone Bodies Attenuate Wasting In Models Of Atrophy, Andrew P. Koutnik, Angela M. Poff, Nathan P. Ward, Janine M. Deblasi, Maricel A. Soliven, Matthew A. Romero, Paul A. Roberson, Carl D. Fox, Michael D. Roberts, Dominic P. D'Agostino

Molecular Pharmacology & Physiology Faculty Publications

Background: Cancer Anorexia Cachexia Syndrome (CACS) is a distinct atrophy disease negatively influencing multiple aspects of clinical care and patient quality of life. Although it directly causes 20% of all cancer-related deaths, there are currently no model systems that encompass the entire multifaceted syndrome, nor are there any effective therapeutic treatments.

Methods: A novel model of systemic metastasis was evaluated for the comprehensive CACS (metastasis, skeletal muscle and adipose tissue wasting, inflammation, anorexia, anemia, elevated protein breakdown, hypoalbuminemia, and metabolic derangement) in both males and females. Ex vivo skeletal muscle analysis was utilized to determine ubiquitin proteasome degradation pathway activation. …

West Florida Shelf Pipeline Serves As Sea Turtle Benthic Habitat Based On In Situ Towed Camera Observations, Heather A. Broadbent, Sarah E. Grasty, Robert F. Hardy, Margaret M. Lamont, Kristen M. Hart, Chad Lembke, Jennifer L. Brizzolara, Steven Murawski

Marine Science Faculty Publications

The use of marine offshore benthic habitats by sea turtles is poorly characterized due to the difficulty of obtaining in situ data. Understanding benthic habitat use that is important to the species’ reproduction, foraging, and migrations is critical for guiding management decisions. A towed camera-based assessment survey system (C-BASS) equipped with environmental sensors was used to characterize and assess benthic habitats on the West Florida Shelf (WFS) from 2014 to 2018. During these cruises, sea turtles were opportunistically observed during the surveys, and critical in situ data such as spatiotemporal information, species identification, habitat use, behavior, and environmental data were …

Guidelines For Tms/Tes Clinical Services And Research Through The Covid-19 Pandemic, Marom Bikson, Colleen A. Hanlon, Adam J. Woods, Bernadette T. Gillick, Leigh Charvet

All publications

The COVID-19 pandemic has broadly disrupted biomedical treatment and research including non-invasive brain stimulation (NIBS). Moreover, the rapid onset of societal disruption and evolving regulatory restrictions may not have allowed for systematic planning of how clinical and research work may continue throughout the pandemic or be restarted as restrictions are abated. The urgency to provide and develop NIBS as an intervention for diverse neurological and mental health indications, and as a catalyst of fundamental brain research, is not dampened by the parallel efforts to address the most life-threatening aspects of COVID-19; rather in many cases the need for NIBS is …

A Possible Path Towards Rapid Development Of Live-Attenuated Sars-Cov-2 Vaccines: Plunging Into The Natural Pool, German Todorov, Vladimir N. Uversky

All publications

Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) is the causative agent of the coronavirus disease 2019 (COVID-19) pandemic spreading around the world, causing massive distress to the world’s economy and affecting healthcare systems worldwide. Although some exposed individuals have no symptoms and most symptomatic infections are not severe, COVID-19 cases span a wide spectrum, ranging from mild to critical and sometimes resulting in life-threatening complications, such as pneumonia, severe respiratory distress and cardiac problems. Currently, there is no curative drug for COVID-19 and vaccines are still under development. We are presenting here a strategy for the fast development of natural …

The Importance Of Research On The Origin Of Sars-Cov-2, Kenneth Lundstrom, Murat Seyran, Damiano Pizzol, Parise Adadi, Tarek Mohamed Abd El-Aziz, Sk. Sarif Hassan, Antonio Soares, Ramesh Kandimalla, Murtaza M. Tambuwala, Alaa A. A. Aljabali, Gajendra Kumar Azad, Pabitra Pal Choudhury, Vladimir N. Uversky, Samendra P. Sherchan, Bruce D. Uhal, Nima Rezaei, Adam M Brufsky

All publications

The origin of the severe acute respiratory syndrome-coronavirus 2 (SARS-CoV-2) virus causing the COVID-19 pandemic has not yet been fully determined. Despite the consensus about the SARS-CoV-2 origin from bat CoV RaTG13, discrepancy to host tropism to other human Coronaviruses exist. SARS-CoV-2 also possesses some differences in its S protein receptor-binding domain, glycan-binding N-terminal domain and the surface of the sialic acid-binding domain. Despite similarities based on cryo-EM and biochemical studies, the SARS-CoV-2 shows higher stability and binding affinity to the ACE2 receptor. The SARS-CoV-2 does not appear to present a mutational “hot spot” as only the D614G mutation has …

Concerning Aligning State Statutes With Federal Law On Hemp, And, In Connection Therewith, Making An Appropriation., Colorado General Assembly

Session Laws 2001-Present

No abstract provided.

Concerning The Provision For Payment Of The Expenses Of The Executive, Legislative, And Judicial Departments Of The State Of Colorado, And Of Its Agencies And Institutions, For And During The Fiscal Year Beginning July 1, 2020, Except As Otherwise Noted., Colorado General Assembly

Session Laws 2001-Present

No abstract provided.

Benefits Of Intermittent Fasting: A Systematic Review Of Randomized Clinical Trials, Kavisha N. Abeyasekera

Physician Assistant Studies | Student Authored Articles

Background: Daily calorie restriction regimens are still the most common diet strategies implemented for weight loss. ^[2,3] In the recent years, intermittent fasting (IMF) has gained popularity among some of the easier diets to follow.^[4]

Objective: The objective of this study is to use the available data on short- and long-term effects of intermittent fasting, either by time restricted feeding or alternate day fasting and help healthcare providers decide on which patients should be recommended IMF as a dietary option.

Study design: Searched Google Scholar, CINAHL, PubMed and Cochrane databases for evidence-based literature on intermittent fasting. Included studies: …

Risks And Benefits Of Using A Commercially Available Ventricular Assist Device For Failing Fontan Cavopulmonary Support: A Modeling Investigation, Masoud Farahmand, Minoo N. Kavarana, Ethan Kung

Publications

Fontan patients often develop circulatory failure and are in desperate need of a therapeutic solution. A blood pump surgically placed in the cavopulmonary pathway can substitute the function of the absent sub-pulmonary ventricle by generating a mild pressure boost. However, there is currently no commercially available device designed for the cavopulmonary application; and the risks and benefits of implanting a ventricular assist device (VAD), originally designed for the left ventricular application, on the right circulation of failing Fontan patients is not yet clear. Moreover, further research is needed to compare the hemodynamics between the two clinically-considered surgical configurations for cavopulmonary …

Cervico-Vaginal Inflammatory Cytokine And Chemokine Responses To Two Different Siv Immunogens, Nikki P.L. Toledo, Hongzhao Li, Robert W. Omange, Tamara G. Dacoba, Jose Crecente-Campo, Dane Schalk, Mohammad A. Kashem, Eva Rakasz, Nancy Schultz-Darken, Qingsheng Li, James B. Whitney, Maria J. Alonso, Francis A. Plummer, Ma Luo

Nebraska Center for Virology: Faculty Publications

Studies have shown that vaccine vectors and route of immunization can differentially activate different arms of the immune system. However, the effects of different HIV vaccine immunogens on mucosal inflammation have not yet been studied. Because mucosal sites are the primary route of HIV infection, we evaluated the cervico-vaginal inflammatory cytokine and chemokine levels of Mauritian cynomolgus macaques following immunization and boost using two different SIV vaccine immunogens. The PCS vaccine delivers 12 20-amino acid peptides overlapping the 12 protease cleavage sites, and the Gag/Env vaccine delivers the full Gag and full Env proteins of simian immunodeficiency virus. We showed …

Cpaf In Focus Vol. 6 Developing Resilience And Managing Uncertainties, Maria Stella E. Noche, Elvie P. Serrano, Mary Ruth O. Rasco, Marriz M. Garciano, Roxanne A. Banalo, Stella Concepcion R. Britanico

IN FOCUS: Development & Governance

In this issue:

• How to best conduct remote teaching: The CPAf experience, 2
• For the love of teaching: Making education work amidst a global pandemic, 4
• How do libraries adapt to new normal?, 6
• Ensuring food-secure future, 8
• How do we help the livestock industry?, 9
• Toward more resilient communities, 10
• Responding to governance and development challenges, 14
• All for community service, 18
• Home-grown leaders, 20

Bosch Creates The First Surreal Painting Of Hell, Sayed Ahmad Shah

Communication Senior Capstones

This paper creates a link to the surrealism art style and "The Garden of Earthly Delights," by Hieronymus Bosch. I wrote this paper for a hell assignment while attending Diablo Valley College. Main point was claiming Hieronymous Bosch to be the first painter to use the surrealism art style.

Pink Is The New Tax, Eliana Burns

Communication Senior Capstones

Historically women in America have made colossal advances that have proven they are just as capable as men. Women had fought and continued to challenge the system since 1919 with the 19th Amendment giving them a right to vote. However, even with this crucial progression, womens’ oppression can be found all around us only in much more subtle ways such as “ the pink tax”. As of 2020 there are currently no federal laws to outlaw companies from charging different prices depending on which gender they are meant to be marketed to. This rhetorical analysis will first address the concept …

Representation In Animation, Michelle L. Benoit

Communication Senior Capstones

In this paper I’m addressing what is missing in representation in films today. I take the shows Avatar: The Last Airbender, Steven Universe and Fairy Tail to explain what proper representation should look like in the media. I look at this with the perspective of rhetoric as a communication major. Providing my comment on the shows. I’m examining this show with three factors. Do they have women in roles of leadership? Are characters overcoming adversity? Are there male characters experiencing complex emotions? I’m comparing these shows with most western films. That would usually tell stories through the white cisgendered straight …

Trinidad State Beach Habitat Restoration Plan, Veronica Leah Farber, Marina Chow, Claudia Rodriguez

Environmental Science & Management Senior Capstones

California State Parks (CSP) is proposing to restore approximately 81 hectares (200 acres) of habitat at Trinidad State Beach (TSB). Over time, invasive non-native plants have established throughout TSB and are encroaching onto sensitive habitats. This project aims to restore coastal shrub communities and native forest understory by the removal of invasive non-native plant species currently inhabiting the area. The purpose of this project is to restore and protect these sensitive habitats while also preventing future degradation of these areas. The project will involve an integrated approach to invasive non-native plant control with treatment methods that will be determined by …

Patrick’S Point State Park Habitat Restoration Plan, Christopher M. Glavan, Marina De Paul, Katie Moore

Environmental Science & Management Senior Capstones

Ongoing efforts at Patrick’s Point State Park (PPSP) seek to restore the ecosystems contained therein. Over time, non-native invasive plant species have become established within the Park. This habitat restoration plan (or Plan) aims to aid in restoring native and sensitive habitat through the removal of non-native invasive species. The Plan will take many different approaches to targeting the invasive non-native plant species depending on the target species and specific site conditions.