Digital Commons Network^™

Metabolic Engineering Of Cyanobacteria For Production Of Chemicals, Po-Cheng Lin

McKelvey School of Engineering Graduate Student Theses & Dissertations

Concerns over the impact of climate change caused by CO2 emission have driven the research and development of renewable energies. Microbial production of chemicals is being viewed as a feasible approach to reduce the use of fossil fuels and minimize the impact of climate change. With recent advances in synthetic biology, microorganisms can be engineered to synthesize petroleum-based chemicals and plant-derived compounds. Cyanobacteria are photosynthetic prokaryotes that use only sunlight, CO2, and trace minerals for growth. Compared to other microbial hosts, cyanobacteria are attractive platforms for sustainable bioproduction, because they can directly convert CO2 into products. However, the major challenge …

Joint Reconstruction For Single-Shot Edge Illumination Phase-Contrast Tomography (Eixpct), Yujia Chen

McKelvey School of Engineering Graduate Student Theses & Dissertations

Edge illumination X-ray phase-contrast tomography (EIXPCT) is an emerging X-ray phasecontrast tomography technique for estimating the complex-valued X-ray refractive index distribution of an object with laboratory-based X-ray sources. Conventional image reconstruction approaches for EIXPCT require multiple images to be acquired at each tomographic view angle. This contributes to prolonged data-acquisition times and elevated radiation doses, which can hinder in vivo applications. In this dissertation, a new “single-shot” method without restrictive assumptions related to the object, imaging geometry or hardware is proposed for joint reconstruction (JR) of the real and imaginary-valued components of the refractive index distribution from a tomographic data …

Metabolic Engineering Of Cyanobacteria For Production Of Chemicals, Po-Cheng Lin

McKelvey School of Engineering Graduate Student Theses & Dissertations

Concerns over the impact of climate change caused by CO2 emission have driven the research and development of renewable energies. Microbial production of chemicals is being viewed as a feasible approach to reduce the use of fossil fuels and minimize the impact of climate change. With recent advances in synthetic biology, microorganisms can be engineered to synthesize petroleum-based chemicals and plant-derived compounds. Cyanobacteria are photosynthetic prokaryotes that use only sunlight, CO2, and trace minerals for growth. Compared to other microbial hosts, cyanobacteria are attractive platforms for sustainable bioproduction, because they can directly convert CO2 into products. However, the major challenge …

Mechanosensitive Epithelial Cell Scattering And Migration On Layered Matrices, Christopher Michael Walter

McKelvey School of Engineering Graduate Student Theses & Dissertations

Epithelial cells form multi-layered tissue scaffolding that makes up every organ in the body. Along with epithelial cells, the basement membrane (BM) and connective tissue are composed of various proteins that sculpt the organs and protect them from foreign macromolecules. Epithelial cells respond to various cues, both chemical and mechanical, from their surrounding matrices to aid in maintenance and repair of these layers through degradation and deposition of extracellular matrix (ECM) proteins. In cancer progression, epithelial cells lose their normal function of supporting tissue structure and instead adopt more aggressive behaviors through an epithelial-to-mesenchymal transition (EMT) of their cellular traits. …

Accelerated Estimation And Permutation Inference For Ace Modeling, Xu Chen, Elia Formisano, Gabriëlla A. Blokland, Lachlan T. Strike, Katie L. Mcmahon, Greig I. De Zubicaray, Paul M. Thompson, Margaret J. Wright, Anderson M. Winkler, Tian Ge

School of Medicine Publications

There are a wealth of tools for fitting linear models at each location in the brain in neuroimaging analysis, and a wealth of genetic tools for estimating heritability for a small number of phenotypes. But there remains a need for computationally efficient neuroimaging genetic tools that can conduct analyses at the brain-wide scale. Here we present a simple method for heritability estimation on twins that replaces a variance component model-which requires iterative optimisation-with a (noniterative) linear regression model, by transforming data to squared twin-pair differences. We demonstrate that the method has comparable bias, mean squared error, false positive risk, and …

Combined Correlational Effects Of Motivation, Emotional Intelligence And Human Capital Development On Job Satisfaction Of Librarians In Nigerian Public University Libraries, Japheth Abdulazeez Yaya Dr, Uloma Doris Onuoha Dr

Library Philosophy and Practice (e-journal)

This study investigated the combined correlational effects of motivation, emotional intelligence and human capital development on the job satisfaction of librarians in Nigerian public universities. A correlational survey research design was adopted. The study population consisted of 1,254 librarians in public university libraries in Nigeria, from which 923 were selected using simple random sampling. The research instrument used was a self-developed questionnaire. A response rate of 67.2% was achieved. Data were analysed using descriptive (percentage, mean, average mean and standard deviation) and inferential (ANOVA) statistics. The study revealed significant relationship among the combined contributions of motivation, emotional intelligence and human …

Census Basics And Obtaining Stats From Data.Census.Gov, David J. Drozd, Kanin Reese

CPAR Presents: A Data and Research Series for Community Impact

Designed for first-time conference attendees or as a refresher, this session will cover important census concepts such as race versus ethnicity, large and small geographies, how and when data are released, and key terminology. Examples will be shown using the Census Bureau’s new data portal.

Going To Market With Deweyfish: The Journey From Partnership To Commercialisation, Kylie Black, Cornelia Hooper, Ian Castleden, Nader Aryamanesh, Harvey Millar

Proceedings of the IATUL Conferences

In 2016-7, The University of Western Australia Library partnered with researchers in the Australian Research Council’s Centre of Excellence in Plant Energy Biology to produce cropPAL2, a database providing the subcellular locations for proteins in crops significant for food production. The project was funded by the Australian National Data Service as part of its High Value Collections program, with the team consisting of computational biologists, software engineers and a librarian. The project involved many hours of manual article evaluation and data extraction by specialists in the plant species included in cropPAL, and the team decided that developing in-house software could …

Eric: E-Research – Infrastructure And Communication, Manuel Hora

Proceedings of the IATUL Conferences

The eRIC team at the University Library of Technical University of Munich acts as a central hub for questions from our scientists related to research data management. We provide services for all steps of research or forward our customers to specialised units, e.g. for bibliometric analysis, publishing or trainings in information literacy. Our team supports researchers with regard to funding requirements, data management and data publication as well as with project-specific requests.

Two integral platforms for our services are the TUM Workbench for research in progress and the repository mediaTUM, mostly for static data sets and publications. In the paper …

The Impact Of Demographics In The Equity Of Global Literacy Education, Saumendra Nath De

The Reading Professor

The right to education has been recognized as a basic human right in a number of international conventions including the 1948 Universal Declaration of Human Rights. The issues of fairness, equal opportunity, and equity in education, however, continue to plague the educational world. According to the UNESCO Institute for Statistics (2017), there are 750 million illiterate adults in the world, six out of ten children and adolescents are not learning globally, and low-income countries are home to a disproportionately large share of children and adolescents not learning. A plethora of demographic factors affects equity in education. This article discusses how …

Thermal And Physico-Mechanical Characterizations Of Thromboresistant Polyurethane Films, Aaron C. Wilson, Shih-Feng Chou, Roberto Lozano, Jonathan Y. Chen, Pierre F. Neuenschwander

Mechanical Engineering Faculty Publications and Presentations

Hemocompatibility remains a challenge for injectable and/or implantable medical devices, and thromboresistant coatings appear to be one of the most attractive methods to down-regulate the unwanted enzymatic reactions that promote the formation of blood clots. Among all polymeric materials, polyurethanes (PUs) are a class of biomaterials with excellent biocompatibility and bioinertness that are suitable for the use of thromboresistant coatings. In this work, we investigated the thermal and physico-mechanical behaviors of ester-based and ether-based PU films for potential uses in thromboresistant coatings. Our results show that poly(ester urethane) and poly(ether urethane) films exhibited characteristic peaks corresponding to their molecular configurations. …

The Journey Of A Virtuous Procrastinator: A Master's Portfolio, Jaclyn Bowlin

Master of Arts in English Plan II Graduate Projects

This portfolio explores several major areas of English and how they can best be taught within English classes. “Reading and Writing with Images: A Guide to Integrating Images into the ELA Classroom” is a major research essay which explores the available avenues of visual rhetoric. A teaching unit plan “Gender and Grammar: A Lesson Plan in Reading, Writing, Speaking, and Listening” integrates grammar instruction into a reading and writing class, while exploring gender as a literary approach. “Textspeak in the English Classroom: Beneficial in Isolated Assignments” contains the findings and analysis of a personal teacher research project conducted with the …

Technical Communication: More Than Writing Or Speech, Laura M. Dannebohm

Master of Arts in English Plan II Graduate Projects

This portfolio fulfills the requirements for a Master of Arts degree in English with a specialization in Professional Writing and Rhetoric. Following an analytical narrative about the collection, the first selection in the portfolio begins as a book analysis and ends with an examination of research on the effects of texting on student grammar. The second selection addresses a scholarly resource gap in technical writing and fills that gap with a review of the Backward Design model for curriculum construction and learning theory that supports the model, and further provides a sample lesson showcasing how to apply the model to …

Screening For Genes That Accelerate The Epigenetic Aging Clock In Humans Reveals A Role For The H3k36 Methyltransferase Nsd1, Daniel E. Martin-Herranz, Erfan Aref-Eshghi, Marc Jan Bonder, Thomas M. Stubbs, Sanaa Choufani, Rosanna Weksberg, Oliver Stegle, Bekim Sadikovic, Wolf Reik, Janet M. Thornton

Paediatrics Publications

Background: Epigenetic clocks are mathematical models that predict the biological age of an individual using DNA methylation data and have emerged in the last few years as the most accurate biomarkers of the aging process. However, little is known about the molecular mechanisms that control the rate of such clocks. Here, we have examined the human epigenetic clock in patients with a variety of developmental disorders, harboring mutations in proteins of the epigenetic machinery. Results: Using the Horvath epigenetic clock, we perform an unbiased screen for epigenetic age acceleration in the blood of these patients. We demonstrate that loss-of-function mutations …

Fy20 Community Day Presentation | Scholarship & Innovation: An Inquiry 2018-2019, José M. Torres, Kelly Page, Amber Stitziel Pareja, Jean Bigger, Richard Palmer, Lindsey Herlehy, Richard Schultz, Nadia Miskowiec

Scholarship & Innovation Committee

Presentation at Community Day | 8.14.2019

Um Hires New Faculty Development Director, Shea Stewart

University of Mississippi News

Josh Eyler looks to improve campus critical thinking

A Comparison Of Individual Learning And Social Learning In Zebrafish Through An Ethorobotics Approach, Yanpeng Yang, Romain J. G. Clément, Stefano Ghirlanda, Maurizio Porfiri

Publications and Research

Social learning is ubiquitous across the animal kingdom, where animals learn from group members about predators, foraging strategies, and so on. Despite its prevalence and adaptive benefits, our understanding of social learning is far from complete. Here, we study observational learning in zebrafish, a popular animal model in neuroscience. Toward fine control of experimental variables and high consistency across trials, we developed a novel robotics-based experimental test paradigm, in which a robotic replica demonstrated to live subjects the correct door to join a group of conspecifics. We performed two experimental conditions. In the individual training condition, subjects learned the correct …

Utilizing Temporal Information In The Ehr For Developing A Novel Continuous Prediction Model, Kang Lin Hsieh

Dissertations and Theses (Open Access)

Type 2 diabetes mellitus (T2DM) is a nation-wide prevalent chronic condition, which includes direct and indirect healthcare costs. T2DM, however, is a preventable chronic condition based on previous clinical research. Many prediction models were based on the risk factors identified by clinical trials. One of the major tasks of the T2DM prediction models is to estimate the risks for further testing by HbA1c or fasting plasma glucose to determine whether the patient has or does not have T2DM because nation-wide screening is not cost-effective.

Those models had substantial limitations on data quality, such as missing values. In this dissertation, I …

Investigating How Faculty Social Networks And Peer Influence Relate To Knowledge And Use Of Evidence-Based Teaching Practices, J. P. Ziker, B. Earl, S. E. Shadle

Anthropology Faculty Publications and Presentations

Background: Calls for science education reform have been made for decades in the USA. The recent call to produce one million new science, technology, engineering, and math (STEM) graduates over 10 years highlights the need to employ evidence-based instructional practices (EBIPs) in undergraduate STEM classes to create engaging and effective learning environments. EBIPs are teaching strategies that have been empirically demonstrated to positively impact student learning, attitudes, and achievement in STEM disciplines. However, the mechanisms and processes by which faculty learn about and choose to implement EBIPs remain unclear. To explore this problem area, we used social network analysis to …

Factors Related To Intra-Tendinous Morphology Of Achilles Tendon In Runners, Kai-Yu Ho, Ari Baquet, Yu-Jen Chang, Lung-Chan Chien, Michelle Harty, Gregory Bashford, Kornelia Kulig

Physical Therapy Faculty Research

The purpose of this study was to determine and explore factors (age, sex, anthropometry, running and injury/pain history, tendon gross morphology, neovascularization, ankle range of motion, and ankle plantarflexor muscle endurance) related to intra-tendinous morphological alterations of the Achilles tendon in runners. An intra-tendinous morphological change was defined as collagen fiber disorganization detected by a low peak spatial frequency radius (PSFR) obtained from spatial frequency analysis (SFA) techniques in sonography. Ninety-one runners (53 males and 38 females; 37.9 ± 11.6 years) with 8.8 ± 7.3 years of running experience participated. Height, weight, and waist and hip circumferences were recorded. Participants …

Design And Architecture Of An Ontology-Driven Dialogue System For Hpv Vaccine Counseling, Amith Faheem Muhammad

Dissertations and Theses (Open Access)

Speech and conversational technologies are increasingly being used by consumers, with the inevitability that one day they will be integrated in health care. Where this technology could be of service is in patient-provider communication, specifically for communicating the risks and benefits of vaccines. Human papillomavirus (HPV) vaccine, in particular, is a vaccine that inoculates individuals from certain HPV viruses responsible for adulthood cancers - cervical, head and neck cancers, etc. My research focuses on the architecture and development of speech-enabled conversational agent that relies on series of consumer-centric health ontologies and the technology that utilizes these ontologies. Ontologies are computable …

Pengaruh Dosis Kascing Dan Bioaktivator Terhadap Pertumbuhan Dan Hasil Tanaman Sawi (Brassica Juncea L.) Yang Dibudidayakan Secara Organik, Agus Wahyudin, Aep Wawan Irwan

Jurnal Kultivasi

Peningkatan produktivitas sawi (Brassica juncea L.) dapat dilakukan secara organik. Tujuan penelitian ini untuk mengetahui pengaruh dosis kascing dan penggunaan bioaktivator terhadap pertumbuhan dan hasil tanaman sawi. Rancangan yang digunakan adalah Rancangan Acak Kelompok (RAK), terdiri atas sembilan kombinasi perlakuan dan diulang empat kali, masing-masing kombinasi perlakuan adalah: tanpa kascing dan tanpa bioaktivator; kascing 5 ton/ha dan tanpa bioaktivator; kascing 5 ton/ha dan bioaktivator 4 mL/L; kascing 10 ton/ha dan tanpa bioaktivator; kascing 10 ton/ha dan bioaktivator 4 mL/L; kascing 15 ton/ha dan tanpa bioaktivator; kascing 15 ton/ha dan bioaktivator 4 mL/L; kascing 20 ton/ha dan tanpa bioaktivator; serta kascing …

Bioluminescent Flashes Drive Nighttime Schooling Behavior And Synchronized Swimming Dynamics In Flashlight Fish, David F. Gruber, Brennan T. Phillips, Rory O'Brien, Vivek Boominathan, Ashok Veeraraghavan, Ganesh Vasan, Peter O'Brien, Vincent A. Pieribone, John S. Sparks

Publications and Research

Schooling fishes, like flocking birds and swarming insects, display remarkable behavioral coordination. While over 25% of fish species exhibit schooling behavior, nighttime schooling has rarely been observed or reported. This is due to vision being the primary modality for schooling, which is corroborated by the fact that most fish schools disperse at critically low light levels. Here we report on a large aggregation of the bioluminescent flashlight fish Anomalops katoptron that exhibited nighttime schooling behavior during multiple moon phases, including the new moon. Data were recorded with a suite of low-light imaging devices, including a high-speed, high-resolution scientific complementary metal-oxide-semiconductor …

Laboratory Animal Welfare And Human Attitudes: A Cross-Sectional Survey On Heterospecific Play Or “Rat Tickling”, Megan R. Lafollette, Sylvie Cloutier, Colleen Brady, Brianna N. Gaskill, Marguerite E. O'Haire

Purdue University Libraries Open Access Publishing Fund

Introduction

Laboratory rat welfare is critically influenced by laboratory animal personnel through their implementation, or lack of implementation, of various enrichment techniques. One such promising technique is heterospecific play, or “rat tickling”, which mimics aspects of rat rough-and-tumble play and can contribute to improving welfare, but may be infrequently implemented. The theory of planned behavior can be used to study implementation by measuring intentions and beliefs about rat tickling, including behavioral attitudes (whether it is good or bad), subjective norms (whether there is social/professional pressure to provide it), and control beliefs (whether they feel in control of providing it). Therefore, …

Investigating How Faculty Social Networks And Peer Influence Relate To Knowledge And Use Of Evidence-Based Teaching Practices, A. K. Lane, John Skvoretz, J. P. Ziker, Brian A. Couch, B. Earl, J. E. Lewis, J. D. Mcalpin, L. B. Prevost, S. E. Shadle, Marilyn Stains

School of Biological Sciences: Faculty Publications

Background: Calls for science education reform have been made for decades in the USA. The recent call to produce one million new science, technology, engineering, and math (STEM) graduates over 10 years highlights the need to employ evidence-based instructional practices (EBIPs) in undergraduate STEM classes to create engaging and effective learning environments. EBIPs are teaching strategies that have been empirically demonstrated to positively impact student learning, attitudes, and achievement in STEM disciplines. However, the mechanisms and processes by which faculty learn about and choose to implement EBIPs remain unclear. To explore this problem area, we used social network analysis …

Bioluminescent Flashes Drive Nighttime Schooling Behavior And Synchronized Swimming Dynamics In Flashlight Fish, David F. Gruber, Brennan Phillips, Rory O'Brien, Vivek Boominathan, Ashok Veeraraghavan, Ganesh Vasan, Peter O'Brien, Vincent A. Pieribone, John S. Sparks

Ocean Engineering Faculty Publications

Schooling fishes, like flocking birds and swarming insects, display remarkable behavioral coordination. While over 25% of fish species exhibit schooling behavior, nighttime schooling has rarely been observed or reported. This is due to vision being the primary modality for schooling, which is corroborated by the fact that most fish schools disperse at critically low light levels. Here we report on a large aggregation of the bioluminescent flashlight fish Anomalops katoptron that exhibited nighttime schooling behavior during multiple moon phases, including the new moon. Data were recorded with a suite of low-light imaging devices, including a high-speed, high-resolution scientific complementary metal-oxide-semiconductor …

Pengaruh Asam Giberelat (Ga3) Dan Waktu Panen Terhadap Kualitas Hasil Buah Zukini (Cucurbita Pepo L.), Kusumiyati, Syariful Mubarok, Ine Elisa Putri, Risa Nurul Falah

Jurnal Kultivasi

Zukini merupakan sayuran buah yang banyak diminati oleh konsumen. Sayuran ini memiliki kadar air yang tinggi. Petani memerlukan teknik budidaya yang tepat untuk meningkatkan kualitas buah zukini, diantaranya dengan penggunaan hormon giberelin (GA3) dan waktu panen yang tepat. Tujuan dari penelitian ini adalah untuk mengetahui efek penggunaan GA3 dan perbedaan waktu panen terhadap kualitas hasil buah zukini, yaitu nilai total padatan terlarut (TPT), kekerasan, dan kadar air buah. Penelitian menggunakan metode percobaan rancangan acak lengkap (RAL) factorial, dengan 2 faktor dan 5 ulangan. Faktor pertama yaitu konsentrasi GA3 (0 part per million (ppm) dan 300 ppm) dan faktor kedua yaitu …

Adventures In Structureland: Exploring Protein Conformational Plasticity By Nmr, Anne R. Kaplan

Doctoral Dissertations

Proteins are complex macromolecules that play a critical role in all biological processes. Their ability to change shape, or conformation, in response to environmental factors is intimately linked with function. Thus, understanding the mechanisms underlying conformational changes is imperative for complete understanding of protein function(s), and an overall comprehension of biological systems. This work explores the structural and dynamic attributes of conformational exchange in the C-terminal domain of pore-forming toxin Hemolysin II (HlyIIC), and the coiled-coil leucine zipper, GCN4p, using nuclear magnetic resonance (NMR). The HlyIIC domain contains a sole proline at position 405 that leads to equal populations of …

Pengaruh Komposisi Media Tanam Dan Waktu Aplikasi Zat Pengatur Tumbuh Terhadap Pertumbuhan Dan Hasil Benih Kentang (Solanum Tuberosum L.) G2 Kultivar Medians Di Dataran Medium Jatinangor, Jajang Sauman Hamdani, Tessa Prima Dewi, Wawan Sutari

Jurnal Kultivasi

Kentang termasuk jenis sayuran komersial bernilai tinggi yang dapat dijadikan sebagai pangan alternatif. Rendahnya ketersediaan benih kentang dapat berpengaruh terhadap produksi kentang. Terbatasnya lahan penanaman kentang di dataran tinggi sehingga diperlukan ekstensifikasi di dataran medium melalui rekayasa media tumbuh dan hormon tumbuh yang bisa mengurangi efek negatif penanaman kentang di dataran medium. Percobaan ini bertujuan untuk mengetahui interaksi antara komposisi media tanam dengan waktu aplikasi zat pengatur tumbuh terhadap pertumbuhan dan hasil benih kentang G2 kultivar Medians di dataran medium Jatinangor. Percobaan dilaksanakan di Kebun Percobaan Ciparanje, Fakultas Pertanian, Universitas Padjadjaran, Jatinangor. Rancangan percobaan yang digunakan yaitu Rancangan Acak Kelompok …

Keragaman Dan Kelimpahan Arthropoda Pada Tajuk Tanaman Cabai Merah Keriting (Capsicum Annuum L.) Varietas Tm 999 Yang Diberi Aplikasi Insektisida Klorantraniliprol 35%, Sudarjat, Annisa Handayani, Siska Rasiska, Wawan Kurniawan

Jurnal Kultivasi

Penelitian ini bertujuan untuk mengetahui keragaman dan kelimpahan arthropoda yang terdapat pada tajuk tanaman cabai yang diberi aplikasi insektisida klorantraniliprol 35%. Metode yang digunakan adalah metode eksperimen dengan rancangan acak kelompok dengan enam perlakuan dosis insektisida klorantraniliprol 35%. Hasil dari penelitian ini adalah didapatkan keragaman pada pengamatan langsung sebanyak 5 ordo, 7 famili dan 7 spesies untuk herbivor, 3 ordo, 4 famili dan 4 spesies untuk predator, serta 1 ordo, 1 famili dan 1 spesies untuk serangga netral. Keragaman yang didapatkan pada pemasangan perangkap kuning yaitu 4 ordo, 4 famili dan 4 spesies untuk herbivor, untuk serangga netral terdiri dari …