Digital Commons Network^™

Development Of Biosensor Using Pyrrolidinyl Pna For Screening Of Hepatitis C Virus Dna, Narathorn Nisab

Chulalongkorn University Theses and Dissertations (Chula ETD)

In this work, a new paper-based analytical device (PAD) that relied on fluorescence measurement was developed for label-free detection of hepatitis C virus (HCV) DNA. Pyrrolidinyl peptide nucleic acid (acpcPNA) was covalently modified onto the patterned cellulose paper to act as a specific probe for capturing the HCV DNA target according to Watson−Crick base-paring rules. The single-stranded (ssDNA)-specific dye which can be electrostatically attached to the surface-bound DNA was employed as the signaling element for the fluorescence-based detection via a smartphone gadget and an iOS application. To acquire the optimal sensitivity, several experimental parameters, namely acpcPNA probe concentration, hybridization time, …

Chemical Constituents And Antibacterial Activity Of Rhizomes From Globba Schomburgkii Hook.F., Naruemon Suekaew

Chulalongkorn University Theses and Dissertations (Chula ETD)

Globba schomburgkii Hook.f. rhizomes were extracted with dichloromethane and fractionated using column chromatography to obtain 10 fractions (FA-FJ). The most active fractions, FE and FG, were further fractionated to give seven and thirteen sub-fractions, respectively. The fractions and sub-fractions were tested for antibacterial activity using 96-well plate microdilution method. The antibacterial activity of several sub-fractions (such as FE.B, FE.C, FE.D, FG.D, FG.G, and FG.H) were higher than that of the crude extract. Chemical compositions of all fractions and sub-fractions were analyzed by gas chromatography hyphenated with mass spectrometry (GC-MS), revealing 167 compounds identified according to match between the experimental MS …

Development Of Thin-Layer Chromatography For Selective Analysis Of Volatile Compounds Using Gas Chromatography Mass Spectrometry, Nattapat Suchatanugal

Chulalongkorn University Theses and Dissertations (Chula ETD)

In this study, simple and selective solid phase extraction approach for a range of volatile compounds in perfume was developed prior to analysis with headspace solid phase micro extraction (HS-SPME) and gas chromatography hyphenated with mass spectrometry (GC-MS). The technique relies on use of thin layer chromatography (TLC) for separation of compound along the silica gel plate followed by selective cuts of the regions of interest, desorption, HS-SPME and GC-MS analysis. The TLC separation of standard compounds using hexane and ethyl acetate (6:1 volume ratio) as the mobile phase was initially performed. The compounds was then desorbed from the TLC …

Structure And Hydration Property Of Low Molecular Weight Hyaluronic Acid By Molecular Dynamics Simulations, Panyakorn Taweechat

Chulalongkorn University Theses and Dissertations (Chula ETD)

Hyaluronic acid (HA) is a biopolymer of disaccharide with two alternate glycosidic bonds, β(1,3) and β(1,4). It has a wide-range of applications in medicine, nutrition and cosmetics. A molecular dynamics (MD) study in aqueous condition presented here unveiled conformational variability in association with the flexibility of the glycosidic linkers, which depends on the number of disaccharide units. HA chain maintains a rigid rod-like conformation with short chain lengths i.e. with 1 to 10 disaccharide units. Crossover from a rod-like to a random-coil conformation is observed with increasing the chain length i.e. with 20-50 disaccharide units. MD and DFT calculations demonstrated …

Detection Of Cyanide Ions Using Chemosensing Ensembles Under Indicator Displacement Assays, Ratanakorn Teerasarunyanon

Chulalongkorn University Theses and Dissertations (Chula ETD)

Anion recognition is an attractive area of research in recent decades as anions play an important role in a wide range of chemical, biological, and environmental processes. This dissertation reports three projects dedicated to anion recognition with applications in fluoride and cyanide sensing. The first project of the dissertation describes the chemistry of two novel anthracene derivatives bearing one and two dimesitylboryl substituents (compounds 1 and 2, respectively) for the complexation of anions. All investigations provide insights into the electronic structure and effect of fluoride and cyanide binding. The results demonstrate that these boranes have a high affinity for fluoride …

Development Of Paper-Based Colorimetric Sensor For Determination Of Human Papillomavirus Dna, Sarida Naorungroj

Chulalongkorn University Theses and Dissertations (Chula ETD)

In this work, a paper-based analytical device (PAD) based on colorimetric assay using pyrrolidinyl peptide nucleic acid (acpcPNA) probe was developed as a sensor for the detection of Human papillomavirus (HPV) DNA. Dextrin-stabilized gold nanoparticles (d-AuNPs) was employed as a colorimetric reagent. The aggregation of d-AuNPs can be induced by positively charged acpcPNA generating a distinctive color change. After the hybridization of acpcPNA and DNA target, the residual acpcPNA probe can cause different degrees of the d-AuNPs aggregation, resulting in the detectable color change. The different color change before and after the introduction of the DNA target as a function …

Determination Of Perchlorate And Thiocyanate Ions By Ion-Selective Membrane And Bulk Optode Techniques Using Experimental Design Approach, Supacha Wirojsaengthong

Chulalongkorn University Theses and Dissertations (Chula ETD)

This research aimed to develop the detection method for perchlorate and thiocyanate in aqueous solutions using potentiometric and colorimetric techniques. The optimum conditions were designed by the central composite design (CCD) with response surface methodology (RSM). The potentiometric response of perchlorate‒selective electrode using di‒tripodal amine calix[4]arene copper(II) complex (L1-Cu) in PVC membrane was studied. The optimized pH, ion exchanger and ionophore concentrations were found at pH 8.0, 3.25 mmol/kg and 13.25 mmol/kg, respectively. The optimized condition performed a Nernstian slope of –58.02 mV/decade with R2=0.9994 in a working concentration range of 1.00×10‒6 – 1.00×10‒2 M, and the detection limit was …

Development Of Fluorinated Rhodacyanine Analogues For Anti-Leishmaniasis, Thitiya Lasing

Chulalongkorn University Theses and Dissertations (Chula ETD)

Recently, fluorinated rhodacyanine has been disclosed as a highly effective agent against Leishmania donovani; it was shown that replacement of hydrogen with a fluorine atom on a benzothiazole unit significantly enhanced the anti-leishmanial activity. However, the role of the fluorine substituent in this analogue has not yet been clarified and mechanism of actions remains unclear. In this research, fifteen novel and three known fluorine-containing rhodacyanine analogues (10c, and 11a-11q) were synthesized and tested the anti-leishmanial activity against promastigote and axenic amastigote stages of L. martiniquensis and L. orientalis, the indigenous Leishmania species of Thailand. The SAR knowledge of this series …

Bioactive Compounds From Lichen Usnea Aciculifera Vain And Usnic Acid Conjugates, Truong Tuong Lam

Chulalongkorn University Theses and Dissertations (Chula ETD)

Phytochemical investigation of lichen Usnea aciculifera Vain led to the isolation of nine new dimeric xanthones, usneaxanthones A-I (36–44), along with 36 known compounds (1–35, 45). The chemical structures of the isolated compounds were elucidated by a combination spectroscopic data (1D, 2D NMR, HRESIMS), ECD experiments, and single-crystal X-ray as well as comparison of their NMR data with those in the literature. The biological activities of isolated compounds were evaluated for antibacterial, anti-dengue and cytotoxic activites. The results revealed that depsides may have potential as lead compounds for the development of new antibacterial and anti-dengue agents. Futhermore, dimeric xanthones exhibited …

An Application Of Line Of Balance And Building Information Modeling For Optimal Resource And Schedule: A Case Study Of An Elevated Highway Construction, Thanakon Uthai

Chulalongkorn University Theses and Dissertations (Chula ETD)

Project scheduling is an essential tool to support construction operations completing the project under limited time and cost. The linear infrastructure project such as elevated highway construction involves a large scale of working area and complexity of management. Recently, schedules are planned manually based on planners’ experience and intuition which may consume time and lead to human-errors. Moreover, the presentations of schedule by using the existing methods dose not cover the overview of projects in various aspects. The objective of this research is to establish an application of Line of Balance (LOB) and Building Information Modeling (BIM) for optimal resource …

Development Of Paper-Based 3d Cell Culture Devices And Real-Time Biosensing Applications, Naricha Pupinyo

Chulalongkorn University Theses and Dissertations (Chula ETD)

Due to its micro fibrous pores, paper has gained traction in the use as a scaffold for 3D cell culture. To study melanoma mechanism, screening the amount of melanin production and invasion study are major keys for drug development. In this work, we developed paper-based devices for culturing melanoma cells. Through the development process, we obtained the optimal parameters, which were applied for 3 different projects; 1) Anti-melanogenic effect screening, 2) Impedance-based E-screen assay, and 3) A membrane insert for real-time invasion assay. As a result of melanin production from melanocytes, the cells can be easily observed after a few …

Effects And Mechanisms Of Caesalpinia Mimosoides Leaf Extract On Oxidative Stress Resistance And Anti-Aging Activity In Caenorhabditis Elegans And Neurite Outgrowth Activity In Neuro2a Cells, Panthakarn Rangsinth

Chulalongkorn University Theses and Dissertations (Chula ETD)

World’s population of older people is rising, dietary supplements promoting healthy lifespan are needed. Moreover, Alzheimer’s disease (AD), an age-related degenerative disease, becomes a public health problem in aging society. Recent reports using cell culture models of AD suggest that amyloid precursor protein (APP), a protein causally related to AD, plays an important role as an inhibitor of neurite outgrowth. Medicinal herbs with neurite outgrowth stimulatory effect may help to prevent and cure AD. Moreover, studies suggest that dietary supplements from plant sources act in preventive nutrition, since they provide antioxidant action against oxidative stress, promote healthspan and prolong lifespan. …

An Affordable Immunohistochemical Approach To Estimate The Prevalence Of Braf V600e Mutation In Papillary Thyroid Cancer Patients Of King Chulalongkorn Memorial Hospital, Thailand., Sonam Choden

Chulalongkorn University Theses and Dissertations (Chula ETD)

Background: Papillary thyroid carcinoma (PTC) accounts for the majority of diagnoses of thyroid carcinoma. BRAFV600E mutation is the most common genetic alteration in PTC, which has diagnostic and prognostic significance. The rate of BRAFV600E mutation in PTC from Thailand has not been reported. Our purpose was to estimate the prevalence of BRAF mutation in a large institutional series using an affordable approach, which combined mutation-specific immunohistochemistry (IHC) with VE1 antibody and tissue microarray (TMA). Methods: A total of 476 PTC cases plotted on TMA were employed for determining the mutation status in this study. The cancer tissue of initial 100 …

Ocean Current Prediction In The Gulf Of Thailand Using Spatio-Temporal Deep Learning On High-Frequency Radar, Nathachai Thongniran

Chulalongkorn University Theses and Dissertations (Chula ETD)

Ocean surface current prediction is a crucial task for a variety of marine activities, such as disaster monitoring, search and rescue operations, power forecasting, and etc. There are three traditional forecasting approaches: (i) numerical based approach, (ii) time series based approach and (iii) machine learning based approach. However, their prediction accuracy was limited as they did not cooperate with spatial and temporal effects together, including oceanic knowledge is also not considered. This paper introduces the ocean surface prediction model that accounts for spatial and temporal characteristics by a combination between CNN and GRU and also the incorporation of oceanic inputs …

In Vitro Safety And Permeation Assessments Of Lidocaine Loaded In Dissolving Microneedle Patch For Ocular Administration, Sirikool Thamnium

Chulalongkorn University Theses and Dissertations (Chula ETD)

The challenge in ocular drug delivery have brought much attention in pharmaceutical research. Efforts have been made to improve the low bioavailability causing by physiological limitations of ophthalmic drug administration. Topical lidocaine solution is a popular choice for ocular treatment, but less than 5% of topically applied dose reached to the target site. Lidocaine loaded in dissolving microneedle patch was considered a good alternative approach to treating eyes. The study aimed to investigate the cytotoxicity of the lidocaine loaded in dissolving microneedle administration against human corneal epithelial cells (HCECs) as well as ex vivo drug target localization with tissue penetration. …

Effect Of Extraction Solvents On Biological Activities Of Murraya Paniculata Leaves In Cosmetic Application, Siriruethai Ruanmai

Chulalongkorn University Theses and Dissertations (Chula ETD)

The objective of this study was to evaluate and compare the antioxidant activity and tyrosinase inhibition activity of Murraya paniculata (Linn.) Jack leaves extract (MPE) obtained from different extraction solvents for the use of cosmetic purposes. The result showed that 75 % ethanolic extract presented the highest antioxidant activity (IC50 = 1.43 mg/mL), the total phenolic content of 66.12 mg GAE/g and the total flavonoids content of 7.83 mg QE/g. All of the obtained extracts had low tyrosinase inhibitory activity. The final MPE lotion contained 1 %w/w of 75 % ethanolic extract dissolved in 50 %w/w of propylene glycol, 20 …

Projection And Determinants Of Cognitive Ability Among Older Persons In Thailand : Role Of Education, Paolo Miguel Vicerra

Chulalongkorn University Theses and Dissertations (Chula ETD)

This thesis examines the role of education on cognitive ability among the older population of Thailand. The nationally-representative data primarily utilised in this thesis is 2016 Population Change and Well-being in the Context of Aging Society. Other datasets had been used in selected themes within this thesis. Multiple analytic approaches had been applied to this study to show various effects of education gradients on cognitive functioning. In the first study, education and other covariates including income, health status, living arrangement, and social participation among others had been analysed by gender to test if the significant factors would be similar. It …

Electro-Deposited Polyaniline On Tio2 As A Photoanode For Improved Photoelectrochemical Performance In Water Oxidation And Methylene Blue, Reeda Jamsri

Chulalongkorn University Theses and Dissertations (Chula ETD)

Polyaniline (PANI) deposited on TiO2 as a photoanode has been synthesized by the electro-polymerization method. The electrodeposition was performed in aqueous solutions containing 0.5 M aniline and 1 M H2SO4 at a constant current density. The photoanode demonstrated its potential applications for photocatalytic degradation of methylene blue dye (MB) and photoelectrochemical (PEC) water splitting. A ~2.7 fold enhancement in the photodegradation activity at 0.9V vs. Ag/AgCl of a bias voltage and the high water oxidation photocurrent density of PANI/TiO2 on FTO photoanode achieves 1.3 mA cm-2 at 0.6V vs. Ag/AgCl under illumination which is higher than that pure TiO2 on …

Incorporating Air-Conditioning Monitoring And Control In Building Energy Management Systems Using Data Science Approach, Pornpra Chumnanvanichkul

Chulalongkorn University Theses and Dissertations (Chula ETD)

This thesis proposes an analytic and control algorithm for a split-type air conditioner (AC) in building energy management systems (BEMS) using data science approach. Available data used in this research are from three sources including Chulalongkorn University Building Energy Management Systems (CUBEMS), Chulalongkorn University Solar Forecast (CUSF) platform and Thai Meteorological Department (TMD). Measured AC power consumption and measured indoor zonal temperature are obtained from CUBEMS. Outdoor weather data are obtained from CUSF platform and TMD. The main procedure of this work can be divided into three processes: four-level AC classification, AC power baseline modeling, and hybrid AC control algorithm. …

Development Of Ecoregion Criteria Through Assessment Of Changing Floating Vegetation: A Case Study Of Intha Communities In Inlelake, Myanmar, Saint N/A

Chulalongkorn University Theses and Dissertations (Chula ETD)

The Inle Lake ecoregion, one of the fresh water ecoregions in the Global 200 list of ecoregions, is facing impacts of sedimentation from upland catchments. Sediment loads and soil sediment characteristics from degraded forest of Kalaw catchment shallowed and reduced plant nutrients in the Inle Lake ecosystems where used to be well recognized for the balancing between Intha livelihoods and natural production of littoral and pelagic zones. This study aimed to assess causes of ecological function change in terms of high sediment loads influencing Intha livelihoods; floating vegetation and to propose criteria for ecoregion development concerning sustainable floating vegetation yields …

การจัดการวัฏจักรไนโตรเจนและฟอสฟอรัสในระบบอควาโปนิกส์, ศิริวณี สุภาจารุวงศ์

Chulalongkorn University Theses and Dissertations (Chula ETD)

การวิจัยนี้มีวัตถุประสงค์เพื่อศึกษาบทบาทของกระบวนการไนตริฟิเคชันในระบบอควาโปรนิกส์ โดยแบ่งการทดลองออกเป็น 3 ส่วน ได้แก่ 1) ศึกษาอัตราการใช้แอมโมเนียและไนเตรทของผักสลัดกรีนโอ๊ค 2) ศึกษาการออกแบบและการทางานของส่วนบำบัดไนตริฟิเคชันสาหรับระบบเลี้ยงสัตว์น้ำแบบปิด และ 3) ศึกษาการประเมินประสิทธิภาพการบำบัดสารประกอบอนินทรีย์ไนโตรเจนและฟอสฟอรัสในระบบเลี้ยงสัตว์น้าแบบปิดที่ทางานร่วมกับระบบอควาโปนิกส์ที่มีและไม่มีการติดตั้งส่วนบำบัดไนตริฟิเคชัน จากการทดลองพบว่า ผักสลัดกรีนโอ๊คสามารถดูดซึมได้ทั้งแอมโมเนียและไนเตรทไปใช้ในการเจริญเติบโต อย่างไรก็ตามพืชจะเลือกใช้แอมโมเนียก่อนไนเตรท ทาให้การดูดซึมไนเตรทสามารถพบได้หลังจากที่แอมโมเนียในระบบหมดลง สำหรับการเพิ่มการสะสมตัวของไนเตรทในระบบเลี้ยงสัตว์น้ำแบบปิดที่ทำงานร่วมกับระบบอควาโปนิกส์สามารถทำได้โดยการติดตั้งส่วนแยกตะกอนและส่วนบำบัดไนตริฟิเคชันด้วยตัวกรองชีวภาพแบบเคลื่อนที่ลงในชุดทดลอง จากการทดลองพบว่าระบบมีอัตราการบำบัดแอมโมเนียเท่ากับ 1.09 มก./ล. และเกิดการเติมอากาศอย่างสมบูรณ์เพื่อเพิ่มประสิทธิภาพการบำบัดไนตริฟิเคชัน โดยเป็นไปตามความคาดหมายว่าในระบบที่มีการติดตั้งส่วนบำบัดไนตริฟิเคชันพบความเข้มข้นของไนเตรทสูงกว่า อย่างไรก็ตามในการเดินระบบเลี้ยงสัตว์น้ำแบบปิดร่วมกับระบบอควาโปนิกส์ในระยะยาว พบว่าการบำบัดไนตริฟิเคชันที่เกิดขึ้นตามธรรมชาติในบ่อเลี้ยงปลาและรางปลูกผักเพียงพอที่จะทำให้เกิดกระบวนการบำบัดอย่างสมบูรณ์เมื่อทำการเดินระบบประมาณ 20 วัน

Exploring The Impact Of Disaster Response Preparedness By The New York State Military Forces To The World Trade Center September 11, 2001, Terrorist Attacks, Robert L. Wolf

Theses and Dissertations

The problem identified in this study concerned the impact of disaster response preparedness (DRP) from the past, present, and future perspectives. The study explored the experiences of four survivors from the 1993 WTC bombing, the effectiveness of seven New York State Military Forces (NYSMF) deployed to Ground Zero (GZ), and perspectives of five Emergency Medical Services (EMS) professionals on DRP operations going forward from 9/11.

Following an explorative heuristic research approach, the study utilized a qualitative and content analysis methodology (both qualitative and quantitative aspects) to investigate the problem using published archival interviews from the informants. Minimally, hypothesis testing, and …

Modeling And Simulation Of Micro/Nano-Rod Distribution In Micro Channel Flows, Saman Monjezi

Doctoral Dissertations

"The behavior of a typical nanorod particle in microscale flows was theoretically investigated, considering the effect of the wall on the rotational and translation motions of the non-spherical particle. Initially, a systematic method using Brownian dynamics simulation of the rotational motion of nanorod was performed to obtain the average orientation distribution of a nanorod in various range of Peclet number and position from the confining wall. Subsequently, the results of the angle distributions simulation were employed to generate a universal mathematical model for the particle orientation distribution, which our model of concentration distribution of high aspect ratio nanorods in the …

Biomaterials For Bone Regeneration, Youqu Shen

Doctoral Dissertations

"The purpose of this Ph.D. research is to investigate and improve two classes of hydroxyapatite (HA)-based biomaterials for bone repair: calcium phosphate microspheres and bioactive silicate glass scaffolds. These biomaterials were prepared with modified compositions and microstructures and then were evaluated for bone regeneration.

The open HA microspheres with dense convex surfaces and rough and porous concave surfaces were obtained by sectioning closed hollow HA microspheres. Bone regeneration with the open HA microspheres was greater than with the closed HA microsphere at 12 weeks. Hollow biphasic calcium phosphate (BCP) microspheres have been prepared with different fractions of HA and β-TCP …

Examining Stigma Associated With Firefighters Seeking Social Support After Experiencing A Traumatic Event, John Richard Tormos

Theses and Dissertations

This applied dissertation was designed to examine stigma associated with firefighters seeking social support after experiencing a traumatic event. This quantitative study employed a survey approach with a cross-sectional design. The researcher self-selected 26 active career firefighters from a medium-sized fire department located in the southeastern United States to participate in the study. Firefighters responded to an array of emergency incidents and were repeatedly exposed to traumatic events. The Secondary Trauma Exposure Scale was employed to answer the first research question: What are the most frequent types of traumatic events that firefighters have experienced? The findings revealed that all of …

Examining The Challenges And Practices Of Our Russian Partners In The Institution Of Teacher Librarianship, Lucy Santos Green, M. P. Johnston

Faculty Publications

Examining international teacher librarianship enables school library researchers and practitioners to continue uncovering the institutional factors that impact school librarianship; further strengthening the case for cross-cultural partnerships. In 2015, researchers conducted an institutional ethnography confirmatory study of school librarianship in Moscow, Russia. Emergent themes culled from previous research and confirmed in this new study were: collaboration, literacy, instruction, technology integration, and the learning environment. Confirmation of these themes in Russian school librarianship indicates teacher librarians around the world struggle with strikingly similar challenges despite varied contexts and socioeconomic situations.

1. The “New Person Contested”: Atheist Humanist Vs. Catholic Worldviews On Transhumanism, Irene J. Dabrowski, Anthony L. Haynor

Praxis Publications

No abstract provided.

Catalog 2019-2020, University Of North Florida

UNF Course Catalogs

No abstract provided.

Enrichment, Characterization And Application Of Bacterial Consortia For Degrading 2-Mercaptobenzothiazole In Rubber Processing Wastewater, Saowaluk Krainara

Chulalongkorn University Theses and Dissertations (Chula ETD)

Benzothiazoles is widely used as vulcanized accelerator in rubber industry. The high volume of 2-MBT usages resulted in its release into the environment and can cause adverse health impacts. This study aimed to obtain efficient 2-MBT-degrading bacteria for wastewater application. The bacterial consortia were enriched by incubating rubber wastewater sludge in a medium containing 2-MBT for 28 days followed by a stepwise acclimation by gradually increasing 2-MBT concentrations in an NH4Cl-containing medium for 76 days. The process significantly increased the bacterial number and changed the dominant populations and degradative genes. Among these consortia, the EN consortium had the highest specific …

Rice Granules As Natural Carrier To Encapsulate Drug And Aromatic Oils, Xue Liu

Chulalongkorn University Theses and Dissertations (Chula ETD)

Rice granules as major energy supply to humanity worldwide are utilized in many fields, such as food, medical, printing and plastic industries. In this thesis work, rice starch/granule is applied as hydrophilic drug (vancomycin) carrier to achieve the release. By confirmation on the stability of rice granules against to various treatments like alkali, acid and heating, as well as a-amylase, trypsin, and lipase, it is possible to use rice granules as 100% natural source to be the capsule and matrix getting the non-core-shell structure of the formation. The success of encapsulation is confirmed by the observations on crystalline, thermal properties …