Digital Commons Network^™

Using A Comparative Approach To Investigate The Relationship Between Landscape And Genetic Connectivity Among Woodland Salamander Population, Alexander C. Camercon, Robert B. Page, James I. Watling, Cari-Ann M. Hickerson, Carl Anthony

2019 Faculty Bibliography

For many amphibian species, reduced landscape connectivity results in reduced genetic connectivity among populations. However, large efective population sizes (Ne) slow the rate of genetic drift, causing subdivided populations to remain genetically similar despite little gene fow among them. Therefore, it is important to address the combined efects of Ne and matrix permeability to quantify the relative importance of gene fow and genetic drift on isolated amphibian populations.

We applied a landscape genetic approach to investigate how patterns of gene fow (m), Ne (inferred via θ) and genetic difer- entiation difer among Eastern Red-backed Salamander (Plethodon cinereus) populations in a …

Species Variation In A Pheromone Complex Is Maintained At The Population Level In The Eastern Red-Backed Salamander, Christina L. Kunkle, Carl Anthony, Cari-Ann M. Hickerson, Richard C. Feldhoff

2019 Faculty Bibliography

Protein pheromones in salamanders of the genus Plethodon have experienced rapid and pervasive directional selection. Variation in mate recognition components, such as the sex-specific pheromones used by plethodontid salamanders, may influence sexual incompatibility and therefore provide a biochemical mechanism for the maintenance of discrete, isolated populations. Recent studies suggest that multiple, genetically distinct lineages of Eastern Red-Backed Salamanders (Plethodon cinereus) are present throughout their broad range. Representative populations from two of these lineages (the Ohio [OH] and Pennsylvania [PA] clades) span the southern shore of Lake Erie in northern Ohio. This distribution pattern creates a unique opportunity to …

Global Temporal Patterns In Plant Nutrient Resorption Plasticity, Rebecca E. Drenovsky, Nicole Pietrasiak, Thomas Short

2019 Faculty Bibliography

Aim Leaf nutrient resorption is a key nutrient conservation trait, which also influences nutrient cycling rates and pools. Most global biogeochemical models assume that resorption is non‐varying at a temporal scale. However, this trait can differ substantially within populations among years. We assessed the commonality of attaining proficient resorption, the factors associated with proficient resorption, as well as the variability of this trait and the factors controlling trait variability.

Scientific Illustration In Biology: Art For Education And Science, L. A. Gaysina, Jeffrey R. Johansen, Christopher Sheil

2019 Faculty Bibliography

Scientific illustration is an important component of biological research. The paper substantiates the importance of supporting a scientific publication, in particular in biology, with original illustrations, considers the features and quality criteria of scientific illustrations. The most accessible techniques of scientific illustration are presented and practical recommendations for making drawings of biological objects are given. The necessity of introducing courses of scientific illustration in the educational programs of Russian universities is noted.

A Snapshot Look At Replication And Statistical Reporting Practices In Psychology Journals, Abdulrazaq Imam, Melissa Frate

2019 Faculty Bibliography

Current efforts started in 2012 by the Association for Psychological Science (APS) appear to be different from previous arguments against null hypothesis statistical testing (NHST), which remained largely rhetorical without specific actions for compliance by researchers in psychology. The APS advocacy involves specific promising implementation tactics. The present study examined the impact of those efforts on replication and statistical reporting practices in four psychology journals from 2011 and 2015. The results showed that amidst increased reporting of NHST statistics in 2015 compared to 2011 and an absence of power reporting in the behavioral journals, there was increased reporting of actual …

Experimental Evidence For Maternal Provisioning Of Alkaloid Defenses In A Dendrobatid Frog, Ralph Saporito, Matthew W. Russell, Corrine L. Richards-Zawacki, Matthew D. Dugas

2019 Faculty Bibliography

Dendrobatid frogs sequester alkaloid defenses from dietary arthropods. Here, we provide experimental evidence that mother strawberry poison frogs (Oophaga pumilio) provision alkaloids to tadpoles. Captive-raised females were fed the synthetic alkaloid decahydroquinoline (DHQ), which we subsequently quantified in their skin, eggs, and developing tadpoles. DHQ quantity was positively associated with tadpole mass/development, suggesting high sequestration rates by tadpoles. These data confirm that tadpoles obtain nutrition and alkaloids by feeding exclusively on maternally provisioned eggs.

Weak Warning Signals Can Persist In The Absence Ofgene Flow, J P. Lawrence, Bibiana Rojas, Antoine Fouquet, Johanna Mappes, Annelise Blanchette, Ralph Saporito, Renan Janke Bosque, Elodie A. Courtois, Brice P. Noonan

2019 Faculty Bibliography

With our comprehensive set of field (model survival), laboratory (controlled learning, palatability, toxin analysis), and molecular data, we provide evidence that polymorphism can persist in an aposematic population, despite expectations of positive frequency-dependent selection. We show that this can happen if prey species carrying a strong signal can exploit predator learning to elicit broad avoidance of many signals, even if predators only have experience with a single signal. This could allow novel signals to be protected within a population of aposematic prey. Thus, under the expectations of broad generalization coupled with limited gene flow, weak aposematic signals can persist, contributing …

A Bridge Too Far In Naming Species: A Total Evidence Approach Does Not Support Recognition Of Four Species In Desertifilum (Cyanobacteria), Laura Gonzalez-Resendiz, Jeffrey R. Johansen, Hilda Leon-Tejera, Leon Sanchez, Marcia Morales

2019 Faculty Bibliography

A population of Desertifilum (Cyanobacteria, Oscillatoriales) from an oligotrophic desertic biotope was isolated and characterized using a polyphasic approach including molecular, morphological, and ecological information. The population was initially assumed to be a new species based on ecological and biogeographic separation from other existing species, however, phylogenetic analyses based on sequences of the 16S rRNA gene and 16S–23S ITS region, placed this strain clearly within the type species, Desertifilum tharense. Comparative analysis of morphology, 16S rRNA gene similarity, 16S–23S ITS secondary structure, and percent dissimilarity of the ITS regions for all characterized strains supports placing the …

Interspecies Sustainability To Ensure Animal Protection: Lessons From The Thoroughbred Racing Industry, Iris Bergmann

Horse racing and competitions

There is a disconnect between dominant conceptions of sustainability and the protection of animals arising from the anthropocentric orientation of most conceptualisations of sustainability, including sustainable development. Critiques of this disconnect are primarily based in the context of industrial animal agriculture and a general model of a species-inclusive conception of sustainability has yet to emerge. The original contribution of this article is two-fold: First, it develops a theoretical framework for interspecies sustainability. Second, it applies this to a case study of the thoroughbred racing industry. Interviews were conducted with thoroughbred industry and animal advocacy informants in the US, Australia and …

Attitudes And Practices Of Australian Veterinary Professionals And Students Towards Early Age Desexing Of Cats, Crawford M. Heather, Michael C. Calver

Management - TNR

Surgical desexing of cats is typically carried out after six months of age (Mature Age Desexing, MAD); between 4–6 months (Traditional Age Desexing, TAD); or before four months (EarlyAge Desexing, EAD). We complemented existing surveys of veterinarians’ acceptance of EAD with online and face-to-face surveys, to ascertain the preferred desexing ages for cats and rationale of 957 Australian veterinarians, veterinary nurses, veterinary science students, and veterinary nursing students. A complementary survey of 299 veterinary practice websites across Australia documented any information provided about desexing cats. The most common reason for preferred desexing ages was reducing stray cat populations (30%); 78% …

Ethics, Care, Animals, Mammals, Jennifer A. Mather Ph.D.

General - Animal Welfare

In the last few decades, we have made great strides in recognizing ethics and providing care for animals, but the focus has been mainly on mammals. This stems from a bias of attention not only in research but predominantly in non-scientists’ attention (to ‘popular’ animals), resulting partly from discussion about and depiction of animals in publications addressed to the public. This is somewhat due to political pressure, and can result in uneven conservation efforts and biases in targets for welfare concerns. As a result, there has been a huge backlash again, with concerns about pain sensitivity and welfare in fish, …

Preparing For Life After Birth: Introducing The Concepts Of Intrauterine And Extrauterine Sensory Entrainment In Mammalian Young, David J. Mellor

General - Animal Welfare

Presented is an updated understanding of the development of sensory systems in a wide range of terrestrial mammals, the prenatal exposure of those systems to salient stimuli, and the mechanisms by which that exposure can embed particular sensory capabilities that prepare newborns to respond appropriately to similar stimuli they may encounter after birth. Taken together, these are the constituents of the phenomenon of “trans-natal sensory continuity” where the embedded sensory capabilities are considered to have been “learnt” and, when accessed subsequently, they are said to have been “remembered”. An alternative explanation of trans-natal sensory continuity is provided here in order …

Welfare-Aligned Sentience: Enhanced Capacities To Experience, Interact, Anticipate, Choose And Survive, David J. Mellor

General - Animal Welfare

The focus of this opinion is on the key features of sentience in animals which can experience different states of welfare encapsulated by the new term ‘welfare-aligned sentience’. This term is intended to exclude potential forms of sentience that do not enable animals in some taxa to have the subjective experiences which underlie different welfare states. As the scientific understanding of key features of sentience has increased markedly during the last 10 to 15 years, a major purpose here is to provide up-to-date information regarding those features. Eleven interconnected statements about sentience-associated body functions and behaviour are therefore presented and …

Good Practices For Animal Welfare In Agriculture Development, Janice Cox

UN SDGs

The 2030 Agenda envisages a development model “in which humanity lives in harmony with nature and … other living species are protected[i].” While the relationship between animal welfare, environmental well-being and human development is starting to be discussed and evidenced, there remains very little recognition of this relationship and the crucial role animal welfare plays in sustainable development for people and planet in UN discussions of the Sustainable Development Goals (SDGs). This paper discusses this further, with specific reference to the development of good practice for animal welfare in agricultural development. However, it is recognized that there are also broader …

The Land Of Meat And Potatoes? Exploring Ireland’S Vegan And Vegetarian Foodscape, Corey Lee Wrenn

Dietary Choice and Foods of Animal Origin Collection

While it would not be accurate to suggest that Ireland is a hub of veganism or vegetarianism, too often it is written off as inherently unsympathetic to the ethics of plant-based eating and anti-speciesist politics. While it is true that Irish culture is historically tied to speciesism and its economy is especially dependent upon “meat” and dairy production, Ireland’s relationship with other animals is complex and sometimes forgiving. This essay seeks to bring shape to the Irish vegan ethic, one that can be traced along its history of animism, agrarianism, ascendency, adaptation, and activism. From its pagan roots to its …

Refinement On The Way Towards Replacement: Are We Doing What We Can?, Kathrin Herrmann

Humane Science Movement Collection

No abstract provided.

Retrospective Review Of Anesthetic And Analgesic Regimens Used In Animal Research Proposals, Kathrin Herrmann, Paul A. Flecknell

Experimental Research and Animal Welfare Collection

Pain has a profound effect on an animal’s wellbeing. In Germany, researchers using animals have been legally required to reduce any possible pain, suffering, distress or lasting harm to an absolute minimum since 1972. To evaluate how these provisions have been implemented in practice, an assessment of refinements to experimental techniques was conducted by retrospectively reviewing 684 surgical interventions described in 506 animal research applications that were sent to the German competent authorities for approval in 2010. This paper focuses on the efficacy of proposed anesthesia and peri- and postoperative analgesia. Postoperative analgesia was not proposed for 30% of surgeries. …

Contesting Animal Experiments Through Ethics And Epistemology: In Defense Of A Political Critique Of Animal Experimentation, Arianna Ferrari

Professional Science Research Ethics Collection

Generally, an animal experiment can be defined as an intervention on an animal, which causes suffering, harm, and distress, for scientific purposes. In this definition, animal experiments differ from more general scientific investigations concerning animals, such as observational studies in the wild in the fields of ethology or conservation, in which animals are involved but may not be harmed. Nowadays, the use of the term vivisection, in the case of animal experiments, is very controversial. This term originally referred to the cutting of living bodies for scientific purposes and has a long conceptual history (Maehle, 1992). In ancient times, it …

How Can The Final Goal Of Completely Replacing Animal Procedures Successfully Be Achieved?, Christiane Baumgartl-Simons, Christiane Hohense

Validation of Alternative Methods Collection

Article 23 of European Union (EU) Directive 86/609/EEC required that Member States promote the development and validation of alternative technologies and stated that the European Commission (EC) “shall report before the end of 1987 on the possibility of modifying tests and guidelines” (European Parliament, 1986, Article 23). This Directive was replaced by Directive 2010/63/EU on the protection of animals used for scientific purposes, which now requires that Member States develop and validate alternative approaches much more precisely and specifies that the ultimate objective is the “full replacement of procedures on live animals for scientific and educational purposes, as soon as …

The Moral Status Of Animal Research Subjects In Industry: A Stakeholder Analysis, Sarah Kenehan

Morality and Ethics of Animal Experimentation Collection

The use of non-human animals (hereinafter referred to as animals) in research and testing is a widely accepted practice in many industries. Millions of animals each year are subjected to painful procedures that include everything from physical mutilation to drug addiction. According to the United States Department of Agriculture (usda), over 820,812 animals were experimented on in the United States in 2016 (usda, 2017), though this count does not include rats, mice, or birds, and dubiously relies solely on the self-reporting of laboratories (Humane Society of the United States, 2011; Keen, 2019, Chapter 10 in this Volume). Estimates suggest that …

Political Campaigning: Where Scientific And Ethical Arguments Meet Public Policy, Emily Mcivor

Animals and Public Policy Collection

The ambition of the paradigm shift we seek is vast, and the obstacles we face are intractable. For anyone opposing the use of non-human animals (hereinafter referred to as animals) in research and testing, the story has been the same from the start. Legitimate concern for animals has been all-too-easily dismissed as misguided sentimentality, and powerful vested interests have claimed scientific, economic, and moral superiority. But the ground is shifting. Animal researchers accept the need to provide scientific justification for their choices, and the protection of animals is increasingly recognized as a public good. Concern among citizens has been translated …

How To Evaluate The Science Of Non-Human Animal Use In Biomedical Research And Testing: A Proposed Format For Debate, Ray Greek, Lisa A. Kramer

Validation of Alternative Methods Collection

Over time, the interpretation of science has occasionally been corrupted by vested interest groups, be they financially motivated or ego driven. Scientific consensus and widespread public beliefs usually catch up with the evidence, but this can take a very long time and often costs lives. The use of non-human animals in biomedical research and testing is a scientific endeavor and, as such, can and should be evaluated in light of the best science currently available. But facts that have been accepted in all areas of science are routinely ignored or called into question by well-funded, vested interest groups, compromising the …

Rethinking The 3rs: From Whitewashing To Rights, Charlotte E. Blattner

Humane Science Movement Collection

Few other issues have prompted as many legislators to adopt legal instruction on the “proper” use of non-human animals (hereinafter referred to as animals) in medical and scientific research. Today, the 3Rs (replacement, reduction, and refinement of animals in scientific procedures) are globally accepted by a vast majority of states (Blattner, 2014); and prominent international organizations, such as the World Organisation for Animal Health (Terrestrial Animal Health Code, 2018, Article 7(8)(3)) and the Council of Europe (Convention for the Protection of Vertebrate Animals Used for Experimental and Other Scientific Purposes, 1986, Articles 6(2), 7 and 8). Widespread acceptance of the …

Increasing The Transparency Of Animal Experimentation: An Australian Perspective, Monika Merkes, Rob Buttrose

Animal Law and Legislation Collection

It has been argued that citizen stakeholders would be well served by greater transparency. The Transparency Register of the European Union (eu) (2016), for example, states that “Transparency is […] a key part of encouraging European citizens to participate more actively in the democratic life of the eu”. But why is transparency in non-human animal (hereinafter referred to as animal) research desirable, or indeed vital? Hadley (2012) argues that the public finance much animal research but do not know what impact their taxes and donations have on animals. Furthermore, he suggests that, since “people enjoy the benefits of animal research …

Extrapolation Of Animal Research Data To Humans: An Analysis Of The Evidence, Rebecca Ram

Validation of Animal Experimentation Collection

The ethical arguments against animal experimentation remain ever-strong. In addition, the scientific case against the use of animals in research grows more compelling, with exponential progress in the development of alternative methods and new research technologies. The Dutch authorities recently announced an ambitious, but welcome, proposal to phase out “the use of laboratory animals in regulatory safety testing of chemicals, food ingredients, pesticides and (veterinary) medicines” by 2025, as well as “the use of laboratory animals for the release of biological products, such as vaccines” (Netherlands National Committee for the protection of animals used for scientific purposes, NCad, 2016, p. …

Wasted Money In United States Biomedical And Agricultural Animal Research, Jim Keen

Biomedicine and Animal Models in Research Collection

Biomedical and agricultural animal research uses millions of experimental animals and dozens of animal species each year by choice, precedent, or regulatory mandate in basic and applied life science research and toxicity testing of drugs, chemicals, and consumer products. Animal research is a large component of the international us$270 billion government-subsidized, biomedical industrial ecosystem (Chakma et al., 2014). In the United States (us) and presumably elsewhere, about half of these funds support animal research and testing (Institute of Medicine and National Research Council, 2012). Each year at least 115 million experimental animals (mostly mice and likely a significant underestimate) are …

The Scientific Problems With Using Non-Human Animals To Predict Human Response To Drugs And Disease, Ray Greek, Lisa A. Kramer

Pharmacology and Animal Models in Research Collection

Every year, and in countries around the world, significant time and resources are devoted to the noble cause of developing drugs to treat and cure human disease. With rare exception, drug interventions cannot reach commercialization without safety and efficacy having first been demonstrated in animal models. The intention of regulations, which require the use of animal models in such contexts, is to ensure that only safe and effective drugs end up being used by patients. Similarly, it is standard practice for researchers to employ animal models in their attempts to understand the way diseases present and progress in humans. Unfortunately, …

Replacing Animal Tests To Improve Safety For Humans, Kathy Archibald, Robert Coleman, Tamara Drake

Application of Alternative Methods Collection

In this chapter, we propose a new, pragmatic approach that could accelerate the replacement of most, if not all, regulatory animal tests with superior tests based on human biology. We also propose that changes to the requirements for safety testing, issued by the us Food and Drug Administration (fda), must be made in order to enable the use of superior new tests, which are currently disadvantaged by the outdated language of the regulations. But first, it is imperative to establish some level of understanding of the efficacy of existing animal-based methods in order to know whether any possible replacement is …

Modernizing Biomedical Training: Replacing Live Animal Laboratories With Human Simulation, John Pawlowski, David Feinstein, Marie L. Crandall, Shalin Gala

Biomedicine and Animal Models in Research Collection

This chapter reviews the global trend towards a modernization of biomedical education in favor of simulation-based training methods, which studies confirm improve student learning and transference of applied skills to clinical practice, reduce laboratory costs, and spare animals from harmful procedures.

Humane Education: The Tool For Scientific Revolution In Brazil, Vanessa Carli Bones, Rita De Cássia Maria Garcia, Gutemberg Gomes Alves, Rita Leal Paixão, Alexandro Aluísio Rocha, Karynn Capilé, Róber Bachinski

Humane Education Movement Collection

Despite the predominance of traditional teacher-centered approaches, educators are constantly changing paradigms and social boundaries, by stimulating criticism with active learning based approaches, which are centered on contexts and experiences. Viewing students as individuals enables paradigm changes and inspires new perspectives on established theories and facts. This chapter discusses education in Brazil, with a focus on humane education. Humane education strives to foster compassion and respect for humans, nonhuman animals (hereinafter referred to as animals), and the environment by creating awareness of the needs of others and the effects of our actions. In this chapter we describe the process of …