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Does Chest Compression During Cardiopulmonary Resuscitation Provide Sufficient Cerebral Oxygenation?, Mehmet Aki̇f Yazar, Mehmet Bariş Açikgöz, Adnan Bayram

Turkish Journal of Medical Sciences

Background/aim: Some of the patients suffering from cardiac arrest (CA) remain in a chronic unconscious state in intensive care units (ICUs). The primary aim of this study was to evaluate the efficacy of chest compression (CC) on cerebral oxygenation during cardiopulmonary resuscitation (CPR). As a secondary goal, we attempted to determine the effects of regional cerebral oxygen saturation (rSO2) values on consciousness and the survival rate using the Full Outline of Unresponsiveness (FOUR) scoring method.Materials and methods: This observational preliminary study was carried out with 20 patients with CA who were hospitalized in ICUs. The rSO2 values measured by near-infrared …

Antinociceptive Effect Of Liposomal Bupivacaine Formulations After Intrathecal Administration In Rats, İlker Yi̇ği̇t, Berri̇n Günaydin, Ömer Hakan Emmez, Meci̇t Orhan Uludağ, Nur Banu Bal, İsmai̇l Tuncer Deği̇m

Turkish Journal of Medical Sciences

Background/aim: Based on our previous in vitro study with multilamellar liposomal bupivacaine (MLB) versus bupivacaine alone in artificial cerebrospinal fluid, we aimed to investigate in vivo antinociceptive effect of intrathecal MLB by determining tail flick latency (TFL) time after thermal stimulation in rats. Materials and methods: After preparing MLB and high-yield drug entrapment in liposome (HYDEL) bupivacaine, 18 female Wistar rats were assigned to 3 groups as control (bupivacaine) and study groups (MLB and HYDEL bupivacaine) including 6 rats in each group to administer these drugs intrathecally. Antinociceptive activity was determined in terms of TFL time after thermal stimulation. Maximum …

Tenofovir Disoproxil Fumarate May Not Cause Renal And Bone Toxicity In Chronic Hepatitis B Patients: A Retrospective Cross-Sectional Study, Hati̇ce Rahmet Güner, Zeli̇ha Koçak Tufan, Gülruhsar Yilmaz, Mehmet Akin Taşyaran

Turkish Journal of Medical Sciences

No abstract provided.

The Distribution Of Mefv Mutations In Turkish Fmf Patients: Multicenter Study Representing Results Of Anatolia, Nazi̇fe Şule Yaşar Bi̇lge, İsmai̇l Sari, Di̇lek Solmaz, Abdurrahman Soner Şenel, Hakan Emmungi̇l, Levent Kiliç, Si̇bel Yilmaz Öner, Fati̇h Yildiz, Sedat Yilmaz, Emi̇ne Duygu Ersözlü, Müge Aydin Tufan, Sema Yilmaz, Veli̇ Yazisiz, Yavuz Pehli̇van, Cemal Bes

Turkish Journal of Medical Sciences

Background/aim: The distribution of Mediterranean fever (MEFV) gene mutations in Turkish familial Mediterranean fever (FMF) patients varies according to geographic area of Turkey. There is a need for highly representative data for Turkish FMF patients. The aim of our study was to investigate the distribution of the common MEFV mutations in Turkish FMF patients in a nationwide, multicenter stu dy.Materials and methods: Data of the 2246 FMF patients, from 15 adult rheumatology clinics located in different parts of the country, were evaluated retrospectively. The following mutations have been tested in all patients: M694V, M680I, M694I, V726A, and E148Q.Results: There were …

Irak-4 Rs4251481 Gene Variant: As A Risk Factor On Inflammatory Bowel Disease, Gonca Candan, Resul Kahraman, Hayri̇ye Arzu Ergen

Turkish Journal of Medical Sciences

Background/aim: Abnormal immune response occurs in individuals who have alleles associated with innate and adaptive immune mechanisms that predispose to inflammatory bowel disease (IBD). Interleukin-1 receptor-associated kinase 4 (IRAK-4) involved in the pathway produces cytokines that initiate and maintain inflammation through Toll-like receptors and interleukin-1 receptors on the membranes of innate immune cells are stimulated with antigens. It was aimed to investigate whether IRAK-4 rs3794262 and rs4251481 polymorphisms predispose to IBD and the possible effects of these polymorphisms by examining these gene polymorphisms with the clinic and prognostic parameters of IBD.Material and methods: Real-time PCR technique was used to detect …

The Results Of Nasopharyngeal Cancer Patients Treated By Simultaneous Integrated Boost Technique And Concomitant Chemotherapy, Mehmet Fai̇k Çeti̇ndağ, Ati̇ye Yilmaz Özsavran, Bülent Yalçin, İclal Çeti̇ndağ, Şeyda Türkölmez, Karabeki̇r Ercan, Di̇nçer Yeğen

Turkish Journal of Medical Sciences

Background/aim: To assess the efficacy and side effects of concurrent weekly chemotherapy and radiotherapy with simultaneous integrated boost (SIB) technique for nasopharyngeal cancer (NPC). Materials and methods: A total of 51 consecutive patients with diagnosis of NPC were treated between February 2010 and December 2015. The median dose for PTV70 (range: 60-82) was given in 33 fractions (range: 31-35). Forty-five of the patients received concomitant weekly chemotherapy between 3-7 cycles (median 6). Eleven patients received neoadjuvant and thirty-nine patients received adjuvant chemotherapy. Results: At a median follow-up 43 months (range: 2-76) the estimated 5-year overall survival and disease-free survival were …

The Efficacy And Safety Of Targeted Narrowband Uvb Therapy: A Retrospective Cohort Study, Kübra Esen Salman, İlknur Altunay, Andaç Salman

Turkish Journal of Medical Sciences

Background/aim: Phototherapy is a safe and effective treatment modality for numerous dermatological conditions. Recently, targeted phototherapy modalities have gained importance due to their advantages over conventional phototherapy.This retrospective study aimed to evaluate the safety and efficacy of targeted narrowband UVB phototherapy in patients with dermatological disorders.Materials and methods: This single-center study included 173 patients who were treated with targeted narrowband UVB phototherapy. Demographic features, phototherapy parameters, and adverse effects were evaluated in all patients, and the treatment response was assessed in patients who attended at least one follow-up visit. Results: A total of 173 patients (102 females; 71 males) with …

Optimum Recording Time Of Routine Electroencephalogram For Adults With Epilepsy*, Kemal Tutkavul, Yilmaz Çeti̇nkaya

Turkish Journal of Medical Sciences

Background/aim: This study aimed to reveal the optimum recording time of routine electroencephalogram (EEG) for adults with epilepsy.Materials and methods: In this clinical observational study we investigated features of paroxysms that emerged in EEGs recorded for 45 min in adults with epilepsy.Results: Paroxysms were detected in 38.14% of 97 patients. The probability of occurrence of paroxysm during the first 10 min was found to be statistically significantly low in comparison to the first 30 and 45 min (respectively P = 0.004, P = 0.0001). This probability was found to increase insignificantly when comparing the first 20 min with the first …

Validation And Reliability Study Of The Turkish Version Of The Neuroquality Of Life (Neuro-Qol)-Stigma Scale For Neurological Disorders, Si̇bel Karşidağ, Ni̇lgün Çinar, Şevki̇ Şahi̇n, Nurdan Kotevoğlu, Mi̇runa Florenti̇na Ateş

Turkish Journal of Medical Sciences

Background/aim: Stigma can be defined as a negative perception of chronically ill patients by their relatives or by society, or a similar self-perception by the patients themselves. We aimed to validate the Turkish version of the Neuroquality of Life (Neuro-QoL)-Stigma Scale for neurologic diseases. Materials and methods: Forms were filled out by a total of 152 randomized patients under regular follow-up in the outpatient clinic (29 polyneuropathy, 25 epilepsy, 23 stroke, 24 tension-type headache, 28 multiple sclerosis, 27 Parkinson disease). The forms consisted of the Beck Depression Inventory (BDI), Beck Anxiety Inventory (BAI), WHOQOL-BREF quality of life scale, the Multidimensional …

Cover And Contents

Turkish Journal of Medical Sciences

No abstract provided.

Effect Of Dilutional Anemia That Can Be Treated With Only One Unit Of Red Blood Cell Transfusion On Tissue Oxygenation In Cardiac Surgery Patients, Büşra Tezcan, Demet Bölükbaşi, Alev Şaylan, Sema Turan, Sultan Sevi̇m Yakin, Di̇lek Kazanci, Ayşegül Özgök, Hi̇ja Yazicioğlu

Turkish Journal of Medical Sciences

Background/aim: Cardiac surgery, especially in the presence of cardiopulmonary bypass (CPB), is associated with an inflammatory reaction that may promote microcirculatory alterations, in addition to the general impact on system hemodynamics. Anemia and transfusion make patients more susceptible to the deleterious effects of CPB. In this study, it was aimed to evaluate the effect of dilutional anemia, which is caused by CPB and can be treated with 1?2 units of red blood cell (RBC) transfusion, on global tissue oxygenation parameters in cardiac surgery patients.Materials and methods: This prospective observational study comprised 127 patients who had a relatively stable operation period …

The Impact Of Different Etiologies Of Diminished Ovarian Reserve On Pregnancy Outcome In Ivf-Et Cycles, Runa Ozelci, Oya Aldemi̇r, Serdar Di̇lbaz, Eni̇s Özkaya, İnci̇ Kahyaoğlu, Berna Di̇lbaz, Özlem Moraloğlu Teki̇n

Turkish Journal of Medical Sciences

Background/aim: Diminished ovarian reserve (DOR) represents a major challenge in reproductive medicine, as it is often associated with poor ovarian stimulation response, high cycle cancellation rate, and low pregnancy rate. The aim of the present study is to compare the clinical pregnancy rates in intracytoplasmic sperm injection-embryo transfer (ICSI-ET) cycles in patients with different DOR etiologies. Materials and methods: Patient data were recorded with a computer-based program called Success Estimation Using a Ranking Algorithm (SERA). Overall, 459 patients were divided into 3 groups according to their DOR etiologies (Group A: idiopathic, n = 81; Group B: age-related, n = 294; …

What Have We Learned In Interventional Pulmonology In The Past Decade?, Mehmet Aki̇f Özgül, Erdoğan Çeti̇nkaya, Demet Turan, Efsun Gonca Uğur Chousein, Deni̇z Doğan, Ekrem Cengi̇z Seyhan

Turkish Journal of Medical Sciences

Background/aim: The increasing number of lung diseases and particularly pulmonary malignancies has intensified the need for diverse interventions in the field of interventional pulmonology. In recent years we have seen many new developments and expanding applications in the field of interventional pulmonology. This has resulted in an increased number and variety of performed procedures and differing approaches. The purpose of the present study is to provide information on patient characteristics, range of interventions, complication rates, and the evolving approach of an experienced center for interventional pulmonology. Materials and methods: We retrospectively examined the records of 1307 patients who underwent a …

Neoadjuvant Volumetric Modulated Arc Radiochemotherapy With A Simultaneous Integrated Boost Technique Compared To Standard Chemoradiation For Locally Advanced Rectal Cancer*, Natalia Jankarashvili, Sophio Kakhadze, Maia Topeshashvili, Lasha Turkiashvili, Mariam Tchiabrishvili

Turkish Journal of Medical Sciences

Background/aim: The present study aimed to examine whether the combination of neoadjuvant volumetric modulated arc radiotherapy (VMAT) using simultaneous integrated boost (SIB-VMAT) techniques and chemotherapy with capecitabine is associated with better clinical and dosimetric outcomes compared to the standard treatment.Materials and methods: The study included 59 patients with cT2?T4 rectal cancer. In the standard arm, patients (n = 37) were treated preoperatively with image-guided VMAT plus capecitabine. In the SIB arm, patients (n = 22) were treated with the SIB-VMAT technique plus capecitabine. All patients underwent radical surgical resection after neoadjuvant radiochemotherapy. Results: In the standard arm, cT0N0 was reached …

Mid-Dose Losartan Mitigates Diabetes-Induced Hepatic Damage By Regulating Inos, Enos, Vegf, And Nf-?B Expressions, Fati̇h Oltulu, Ayli̇n Buhur, Çevi̇k Gürel, Gökçe Ceren Kuşçu, Meli̇h Dağdevi̇ren, Nefi̇se Ülkü Karabay Yavaşoğlu, Ti̇mur Köse, Altuğ Yavaşoğlu

Turkish Journal of Medical Sciences

Background/aim: Losartan, an antihypertensive drug, is highly preferred in patients with diabetes mellitus (DM) and hypertension because of its retarding effect on diabetic nephropathy. In this study, we investigated the potential therapeutic effect of different doses of losartan on hepatic damage in a streptozotocin (STZ, 50 mg/kg)-induced DM model in rats.Materials and methods: In this study, five different groups were formed: control, DM, low-dose losartan (5 mg/kg), mid-dose losartan (20 mg/kg), and high-dose losartan (80 mg/kg). Liver tissues of experimental groups were evaluated immunohistochemically for TUNEL, iNOS, eNOS, VEGF, and NF-?B pathways. In addition to immunohistochemical analysis, analyses of SOD …

Investigation Of The Assessment Of Low Degree (<50%) Renal Artery Stenosis Based On Velocity Flow Profile Analysis Using Doppler Ultrasound: An In-Vitro Study, Andrew Malone, Deepa Chari, Sean Cournane, Izabela Naydenova, Andrew J. Fagan, Jacinta Browne

Articles

Purpose: Renal arterial stenosis can lead to disrupted renal function due to reduced blood flow to the kidneys and is largely thought to be caused by atherosclerosis. Current diagnostic strategies for renal arterial stenosis rely on detecting large degree stenoses (>50%). This study aimed to test the viability of using Doppler ultrasound to assess velocity profiles to detect the presence of low degree (< 50%) stenoses.

Methods: A series of anatomically realistic renal artery flow phantoms were constructed exhibiting a range of low degree stenoses (symmetric and asymmetric). The behaviour of fluid flow in the phantoms was examined using Doppler ultrasound …

Synchrotron Macro Atr-Ftir Microspectroscopy For High Resolution Chemical Mapping Of Single Cells, Jitraporn Vongsvivut, David Perezguaita, Bayden Wood, Phil Heraud, Karina Khambatta, David Hartnell, Mark J. Hackett, Mark J. Tobin

Articles

Attenuated total reflection Fourier transform infrared (ATR-FTIR) spectroscopy has been used widely for probing the molecular properties of materials. Coupling a synchrotron infrared (IR) beam to an ATR element using a high numerical aperture (NA) microscope objective enhances the spatial resolution, relative to transmission or transflectance microspectroscopy, by a factor proportional to the refractive index (n) of the ATR element. This work presents the development of the synchrotron macro ATR-FTIR microspectroscopy at Australian Synchrotron Infrared Microspectroscopy (IRM) Beamline, and demonstrates that high quality FTIR chemical maps of single cells and tissues can be achieved at an enhanced spatial …

Ocular Biometry, Refraction And Time Spent Outdoors During Daylight In Irish Schoolchildren, Síofra Harrington, Veronica O'Dwyer

Articles

Background: Previous studies have investigated the relationship between ocular biometry and spherical equivalent refraction in children. This is the first such study in Ireland. The effect of time spent outdoors was also investigated.

Methods: Examination included cycloplegic autorefraction and non-contact ocular biometric measures of axial length, corneal radius and anterior chamber depth from 1,626 children in two age groups: six to seven years and 12 to 13 years, from 37 schools. Parents/guardians completed a participant questionnaire detailing time spent outdoors during daylight in summer and winter.

Results: Ocular biometric data were correlated with spherical equivalent refraction (axial …

Ocular Demodex Folliculorum: Prevalence And Associated Symptoms In An Irish Population, Orla Murphy, Veronica O'Dwyer, Aoife Lloyd-Mckernan

Articles

Purpose To investigate the prevalence of ocular Demodex folliculorum (DF) in an Irish population. To validate a modified Ocular Surface Disease Index questionnaire and employ it to evaluate the relationship between dry eye symptoms and the presence of DF. Methods One hundred and fifty-six subjects were enrolled in an epidemiological cross-sectional prevalence study. Each subject completed a novel questionnaire on ocular symptoms and was assessed for the presence of DF. Data was analysed to assess prevalence and to search for significant links between each symptom and DF. Results An overall prevalence of 68% was found. Total mean number of DF …

Assessment Of Low Doses Of Eimeria Tenella Sporulated Oocysts On The Biochemical Parameters And Intestinal Microflora Of Chickens, Iva Langrová, Darina Chodova, Eva Tumova, Barbora Horakova, Romana Krejcirova, Martina Saskova, Roman Svejstil, Ivana Jankovska, Jaroslav Vadlejch, Zuzana Cadkova, Ivana Knizkova, Stanislava Nechybova

Turkish Journal of Veterinary & Animal Sciences

This study was carried out to evaluate the biochemical parameters and intestinal microflora in broilers with low doses of Eimeria tenella. Birds from the experimental groups were orally infected with 500 (GI) or 1000 (GII) sporulated oocysts of E. tenella and then examined for weight gain, biochemical indicators, and microbiota on day 9 and 16 after infection. Experimental infection of E. tenella on day 9 post infection (pi) increased concentrations of uric acid, cholesterol, triacylglycerols, and glucose; however, these measurements did not differ from those of the control group on day 16 pi. On day 16 pi, bifidobacteria counts were …

Chemical, Enzymatic, And Antioxidant Enrichments Of Full-Fat Soybean And Sunflower Meal By Bacillus Subtilis (Atcc$^{®}$ 6633$^{Tm}$) Fermentation Using A Solid-State Bioreactor, Yaşar Karakurt, Damla Güverci̇n, Sercan Önder, Ci̇van Çeli̇k, Ramazan Tosun, Burak Baran, Sulhatti̇n Yaşar

Turkish Journal of Veterinary & Animal Sciences

This study was conducted to fortify nutritional and chemical composition of sunflower meal (SFM) and full-fat soybean (FFSB) used as a protein source in farm animal nutrition by Bacillus subtilis ATCC 6633 fermentation. An optimized set of fermentation parameters (60% moisture, 6.5-7.0 pH, 30-35 °C temperature, continuous aeration of 0.5-1.0 L/min and agitation of 20-100 rpm) were used to ferment SFM and FFSB samples with or without using bacterial inoculant for 0, 24, 48, and 72 h. The results indicated that the total organic acids (TOA) contents and crude ash (CA) of SFM and FFSB increased significantly, but there were …

Evaluation Of The In Vivo Efficacy Of Pumpkin (Cucurbita Pepo) Seeds Against Gastrointestinal Helminths Of Chickens, Jezie A. Acorda, Imma Ysabela Emille C. Mangubat, Billy P. Divina

Turkish Journal of Veterinary & Animal Sciences

The present study was conducted to evaluate the in vivo efficacy of pumpkin seeds as an alternative natural anthelmintic for chickens. Ninety Philippine Jolo native chickens of mixed sexes, aged 4?5 months and weighing 1?2 kg, were randomly distributed into three treatment groups with 30 chickens per group. Control group A was fed basic mash feed, group B received feed mixed with ground pumpkin seeds (2 g/bird per day), and group C received mebendazole-medicated feed (30 mg/kg body weight). Fifteen randomly selected chickens from each group were euthanized and necropsied before treatment, and the remaining fifteen in each group were …

A Comparative Phylotyping Analysis Of E. Coli Originating From Avian Colibacillosis Based On The Clermont Triplex Scheme And Gyra Gene Sequencing, Mohsen Amiri, Elham Ahmadi

Turkish Journal of Veterinary & Animal Sciences

Avian colibacillosis (AC) is frequently reported from poultry farms in the west of Iran. The aim of the present survey was to evaluate the phylogeny of Escherichia coli isolated from AC through the Clermont triplex protocol and gyrA gene sequencing. One hundred and fifty fresh carcasses suspected of having died from colibacillosis were sampled. The specimens were examined for E. coli isolation and identification. The extracted DNA repertoire of the isolates was subjected to phylogenetic analysis using Clermont triplex PCR. A DNA fragment of 1.8 kbp of the gyrA gene was amplified from a representative of each phylogroup or subgroup …

Management Of Dystocia Due To Unilateral Uterine Torsion In A Labrador Bitch: A Surgical Approach, Sameer Ali Mohammed, Kavitha Kannan, Arunmozhi Narayanasamy, Sankar Palanisamy, Sridevi Purushothaman

Turkish Journal of Veterinary & Animal Sciences

Uterine torsion is an uncommon disorder of bitch and is more likely to occur in the pregnant animals. A three-year-old Labrador bitch was presented to the Madras Veterinary College Teaching Hospital with the history of having delivered two live puppies in the morning with no further signs of parturition. On abdominal palpation, the fetal mass was palpable. Radiography revealed three fetal skeletons and ultrasound revealed viable fetuses. Based on the history and clinical examination, the case was tentatively diagnosed as primary uterine inertia. Even after two inductions, no puppies were delivered and hence to save the life of puppies, emergency …

Old Age-Associated Impairment Of The Rat Liver Antioxidant Defense System: The Basis For Affirmation Of The Experimental Model, Marko Miler, Vladimir Ajdzanovic, Jasmina Zivanovic, Ivana Jaric, Branka Sosic-Jurjevic, Milica Potrebic, Verica Milosevic

Turkish Journal of Veterinary & Animal Sciences

No abstract provided.

Sybr Green-Based Real-Time Pcr Detection Of Canine Babesia Spp. In Ixodid Ticks Infesting Dogs In Kerala, South India, Lavinia Wahlang, Bindu Lakshmanan, Naicy Thomas, Anu Bosewell, Jain Jose, Sunanda Chulliyil, Aravindakshan Thazhathuveetil

Turkish Journal of Veterinary & Animal Sciences

Ticks, being hematophagous ectoparasites, are notorious vectors that transmit an array of pathogenic viruses, bacteria, and protozoa while simultaneously exhibiting a zoonotic potential by transmitting pathogens that affect the health of owners in contact. The distribution of ixodid ticks of the genera Rhipicephalus and Haemaphysalis spp. in tropical climate of the state contributes to many serious tick-borne parasitic and rickettsial infections in domestic and wild canines. In south India, canine babesiosis is one of the most prevalent vector-borne parasitic diseases. Detection of parasites in tick vectors has significant epidemiological implications. The present study was designed to identify the presence of …

Determination Of Antioxidants In Bovine Oviduct Epithelial Cell Culture Isolated At Different Periods Of The Estrous Cycle, Aytül Kürüm, Si̇yami̇ Karahan, Hakan Kocamiş, Mi̇yase Çinar, Emel Ergün

Turkish Journal of Veterinary & Animal Sciences

Oxidative stress interferes with oviduct functions including oocyte maturation, capacitation, fertilization, and embryo and gamete transport. This study aimed to determine activity of the antioxidants glutathione peroxidase (GPX-I), superoxide dismutase (SOD), and catalase (CAT) in bovine oviduct epithelial cells (BOEC) isolated from the isthmus and ampulla of the oviduct at estral (n = 7) and luteal phases (n = 7) of the estrous cycle. The antioxidant activity was measured at the primary, first, and second passages of the cell culture, and was characterized by cytokeratin expression. The GPX activity increased over the passages in samples of the ampulla and the …

A Preliminary Study On Effects Of Subanesthetic Doses Of Preemptive Ketamine Given Prior To Premedication On Total Intravenous Anesthesia For Short- To Medium-Term Surgical Procedures In Horses, Pallvi Sharma, Aswathy Gopinathan, Kiranjeet Singh, Sherin B. Sarangom, Chelladurai Sowbharenya, Christina John, Med Ram Verma

Turkish Journal of Veterinary & Animal Sciences

This study was conducted to determine the effects of subanesthetic doses of ketamine given prior to premedication on the quality of anesthesia, recovery, and postoperative pain in horses. Eighteen horses were randomly recruited into three equal groups, S, LK, and HK, wherein saline, ketamine at 0.2 mg/kg body weight (bwt), and ketamine at 0.4 mg/kg bwt were given, respectively, i.v. for 30 min as a continuous rate infusion (CRI). Horses were premedicated with xylazine at 1 mg/kg bwt i.v. and butorphanol at 0.05 mg/kg bwt i.v. after 30 min. Anesthesia was induced using ketamine and midazolam and was maintained with …

A Possible Correlation Between Diet, Serum Oxytetracycline Concentration, And Onset Of Reproductive Disturbances In Bitches: Clinical Observations And Preliminary Results, Alessandro Di Cerbo, Gianandrea Guidetti, Sergio Canello, Raffaella Cocco

Turkish Journal of Veterinary & Animal Sciences

Based on the increase of food-related disturbances in animals and the recent findings concerning the toxicity of oxytetracycline (OTC) presence within pet food, we compared the effectiveness of two commercially available diets in 36 bitches suffering from reproductive disturbances. We ran a randomized clinical evaluation over a 90-day period. Bitches were randomly assigned to receive a control diet (CD) or a nutraceutical diet (ND) and were assessed for the presence of OTC in their sera before and at the end of the study. Sixteen of 18 bitches in the ND group went into heat within an average of 35.5 ± …

Phylogenetic Analyses Of P32, Rpo30, Gpcr, Orf117, And Kelch-Like Genes Of Tunisian Sheeppox Virus Isolates, Jihene Lachheb, Aicha Turki, Houssem Mastour, Jihene Nsiri, Imen El Behi, Imen Labri, Abdeljelil Ghram

Turkish Journal of Veterinary & Animal Sciences

Sheeppox is an economically important disease of small ruminants caused by the sheeppox virus (SPPV). Sheeppox virus (SPPV), goatpox virus, and lumpy skin disease virus are capripoxviruses, antigenically related but genetically distinct. Their differentiation requires analysis at a molecular level. Eleven sheeppox virus isolates from five different regions of Tunisia were collected between 2010 and 2016. SPPV was detected by real-time PCR using SYBR Green technology. Primers amplifying the entire Kelch-like gene were designed. Then the GPCR, RPO30, P32, ORF117, and Kelch-like genes were molecularly analyzed to show the different specific lineage patterns of each species. Phylogenetic analyses revealed three …