Digital Commons Network^™

Development Of More Light Sensitive And Red-Shifted Channelrhodopsin Variants For Optogenetic Vision Restoration, Tushar Harishkumar Ganjawala

Wayne State University Dissertations

Discovery of channelrhodopsin (ChR), a light sensitive protein from green algae, has revolutionized the field of neuroscience research by empowering scientist to control neuron through the light, the technology popularly known as optogenetics. The ChR based optogenetics is one of the promising approaches for treating blindness caused by photoreceptor degenerative diseases such as retinitis pigmentosa (RP) and age-related macular degeneration (AMD). Fundamentally, the approach is about imparting light sensitivity to surviving inner retinal cells by ectopic expression of genetically encoded light sensitive proteins, such as ChR2. Although the concept of optogenetic approach has been proved by using ChR2, a major …

The Use Of Cultural Algorithms To Learn The Impact Of Climate On Local Fishing Behavior In Cerro Azul, Peru, Khalid Kattan

Wayne State University Dissertations

Recently it has been found that the earth’s oceans are warming at a pace that is 40% faster than predicted by a United Nations panel a few years ago. As a result, 2019 has become the warmest year on record for the earth’s oceans. That is because the oceans have acted as a buffer by absorbing 93% of the heat produced by the greenhouse gases [40].

The impact of the oceanic warming has already been felt in terms of the periodic warming of the Pacific Ocean as an effect of the ENSO process. The ENSO process is a cycle of …

Utilizing Immunopet To Measure Tumor Response To Treatment In Breast Cancer, Brooke Mcknight

Wayne State University Dissertations

With a broad spectrum of therapies available for treating breast cancer, the need for personalized medicine tailoring the cure according to phenotype is evident. Such an approach may be fully realized with the development of quantitative imaging technologies for disease detection, staging and diagnosis, without increasing patient burden. Immuno-positron emission tomography (PET) combines the targeted specificity of antibodies with the sensitivity of PET for whole body imaging by targeting molecular features amplified in lesions. ImmunoPET probes targeting different antigens and their utility to measure response to treatment were explored. 89Zr-trastuzumab was employed as a surrogate readout of Src inhibition after …

The Center Of All Beauty: Radical Democracy, Materiality, And The Poetic Subject In Twentieth-Century American Poetry, Marcus Merritt

Wayne State University Dissertations

In The Center of All Beauty, I trace a strain of poetics in twentieth-century American poetry from William Carlos Williams through Frank O’Hara, Alice Notley, and Amiri Baraka. This poetics is founded in a radically democratic conception of the poetic subject and in the use of poetry as a tool for developing critical knowledge about the material conditions within which the poetic subject is constituted. In Spring and All and The Embodiment of Knowledge, Williams articulates a poetics that denies the authority of any grounds upon which any poetic subject would be considered inadequate to poetic speech and outlines a …

Learning From Heterogeneous Data, Lu Wang

Wayne State University Dissertations

Data with both heterogeneity and homogeneity is now ubiquitous due to the development of multitudinous data collection techniques. To encode the data heterogeneity and homogeneity, we focus on unsupervised and supervised learning approaches. In unsupervised learning, to consider both data heterogeneity and homogeneity, we develop three clustering frameworks to maximize the heterogeneity among data sub-groups and homogeneity within each data sub-group for over-dispersed data in three different data types, i.e., alphabetic, network and mixed feature types data. In supervised learning, the traditional approaches, however, either build a global model for a whole group including all sub-groups, which fail to consider …

“I Was Prepared To Stay Here And Die With My Animals”: Pet Owners, Hurricane Harvey, And The Role Of Communication In Disaster Sense-Making, Ashleigh Day

Wayne State University Dissertations

Companion animals (also referred to as “pets”) are omnipresent in society and, increasingly, many companion animals are considered members of the family unit. However, crisis planning, preparedness, and communication efforts are sparse in including pets and their owners/guardians. This study investigates the disaster experience(s) and sense-making process(es) of pet owners (POs) and animal guardians (AGs) that were affected by the 2017 Hurricane Harvey. More specifically, examination pays close attention to the role and function of PO/AG identity and crisis and risk communication in individuals’ sense-making of their disaster experience. Importantly, participant experiences provide the means to examine the larger context …

Self-Defined: A Womanist Exploration Of Michelle Obama, Viola Davis, And Beyonce Knowles, Idrissa Nichelle Snider

Wayne State University Dissertations

Intersectional research that focuses on the experiences and representations of Black women should place emphasis on examining the communication of resistance. This dissertation builds upon the work of Womanist (Walker, 1983) and Black Feminist scholars (Collins, 1991; Harris-Perry, 2011) in order to identify and interrogate the harmful systemic nature of various stereotypes and controlling images of Black women. These controlling images historically include representations such as the Mammie, Sapphire, Jezebel, tragic mulatto, and even newer images like the angry black woman. Through a close reading of Josephine Baker’s “Danse Sauvage" performance, the research points to modern day examples of when …

Stress-Dependent Regulation Of A Major Node Of The Insulin-Like Peptide Network That Modulates Survival, Rashmi Chandra

Wayne State University Dissertations

Chronic stress disrupts insulin signaling, predisposing human populations to diabetes, cardiovascular disease, Alzheimer’s Disease, and other metabolic and neurological disorders, including post-traumatic disorders (PTSD). Thus, efficient recovery from stress optimizes survival. However, stress recovery in humans is difficult to study, but is much easier to dissect in model organisms. The worm genetic model Caenorhabditis elegans can switch between stressed and non-stressed states, and this switch is largely regulated by insulin signaling. Previously, the Alcedo lab proposed that insulin-like peptides (ILPs), which exist as multiple members of a protein family in both C. elegans and humans, implements a combinatorial coding strategy …

Defining The Effect Of Environmental Perturbation On The Male Germline, Molly Estill

Wayne State University Dissertations

Periconceptional environment, according to the Developmental Origins of Health and Disease (DOHaD) theory, influences offspring phenotype, primarily via epigenetic mechanisms. Although the paternal component in humans is poorly understood, both maternal and paternal peri-conceptional environment are now believed to contribute to this phenomenon. Manipulation of the early embryo for treating human infertility, is suspected of contributing to offspring abnormalities through epigenetic mechanisms. To directly address the effects of common assisted reproductive technology procedures on the offspring epigenome, the DNA methylation profiles of newborns conceived naturally, or through the use of intrauterine insemination (IUI), or in vitro fertilization (IVF) using Fresh …

Biochemical, Structural, And Drug Design Studies Of Aspartate Transcarbamoylase From Pseudomonas Aeruginosa And Staphylococcus Aureus, Chandni Patel

Wayne State University Dissertations

Sepsis affects 1.7 million people in the United States every year and nearly 270,000 people die as a result. Sepsis is characterized by systemic inflammation from an infection leading to organ dysfunction and death. Multi-drug resistance in bacteria is increasing globally, and Pseudomonas aeruginosa and Staphylococcus aureus are notorious for their multi-drug resistance and pose a serious need for the development of new antibiotics. The levels of pyrimidines in blood are too low to sustain the growth of bacteria, so they must rely on pyrimidine biosynthesis. Previous studies have shown that a defect in several pyrimidine biosynthetic enzymes resulted in …

Investigation Of The Role For Methyl-Cpg Binding Protein 2 Variant Mbd2_V2 In Cancer Stem Cells And Obesity-Associated Cancers, Emily A. Teslow

Wayne State University Dissertations

Obesity is a risk factor for both TNBC and PCa, and pro-inflammatory features associated with obesity, including upregulated production of ROS, promote CSCs. Previously published work from the Bollig-Fischer laboratory established that TNBC CSCs could be inhibited by neutralizing ROS in culture with H2O2 targeted antioxidants. In this report, antioxidant treatment resulted in the downregulation of mRNA splicing variant MBD2_v2. MBD2_v2 was highly expressed in CSCs versus bulk TNBC cells and supported self-renewal in vitro. As obesity is coupled with increased ROS, we hypothesized that obesity could drive CSCs via MBD2_v2 expression. The work presented in this thesis addressed this …

The Balance Between Prostaglandin E2 Ep3 And Ep4 Receptors Determines Severity Of Cardiac Damage In Myocardial Infarction And An Angiotensin Ii-Induced Model Of Hypertension, Timothy Dean Bryson

Wayne State University Dissertations

According to the center for disease control about 610,000 people die every year in the United States from heart disease, of which, coronary heart disease is the most common form. One major risk factor for heart attack is hypertension, which affects nearly half of all Americans [472, 473]. PGE2 plays an important role in regulating cardiovascular function and mediating inflammation, both of which contribute to the development of hypertension and/or heart disease. Prostaglandin E2 can act as a vasodilator or vasoconstrictor depending on which of its receptor subtypes are activated.

In general, activation of the EP1 and EP3 receptors is …

Novel Insights Into The Use Of Ercc1 As A Biomarker For Response To Platinum-Based Chemotherapy In Lung Cancer, Joshua Ryan Heyza

Wayne State University Dissertations

ERCC1/XPF is a DNA endonuclease with variable expression in primary tumor specimens, and has been investigated as a predictive biomarker for efficacy of platinum-based chemotherapy in non-small cell lung cancers where up to 30-60% of tumors harbor low to undetectable ERCC1 expression. The failure of an international, randomized Phase III clinical trial utilizing ERCC1 expression to predict response to platinum-based chemotherapy suggests additional mechanisms underlying the basic biology of ERCC1 in the response to platinum-DNA damage remain unknown. In this work, we aimed to characterize a panel of ERCC1 knockout cell lines generated via CRISPR-Cas9 where we identified a synthetic …

Capso: A Multi-Objective Cultural Algorithm System To Predict Locations Of Ancient Sites, Samuel Dustin Stanley

Wayne State University Dissertations

ABSTRACT

CAPSO: A MULTI-OBJECTIVE CULTURAL ALGORITHM SYSTEM TO PREDICT LOCATIONS OF ANCIENT SITES

by

SAMUEL DUSTIN STANLEY

August 2019

Advisor: Dr. Robert Reynolds

Major: Computer Science

Degree: Doctor of Philosophy

The recent archaeological discovery by Dr. John O’Shea at University of Michigan of prehistoric caribou remains and Paleo-Indian structures underneath the Great Lakes has opened up an opportunity for Computer Scientists to develop dynamic systems modelling these ancient caribou routes and hunter-gatherer settlement systems as well as the prehistoric environments that they existed in. The Wayne State University Cultural Algorithm team has been interested assisting Dr. O’Shea’s archaeological team by …

Queer Dispute Resolution, Daniel Del Gobbo

Cardozo Journal of Conflict Resolution

The article is organized as follows. Part H traces the conceptual shift in legal theory from thinking about the juridical subject as a bearer of rights to a bearer of interests. This provides the necessary context for my reframing the bearer of interests as a bearer of desires. Part III begins to explore the implications of this move, drawing on what I call the "ethic of positivity" in parts of queer theory that celebrates the potential for sexual desires to transcend identity categories. This theory suggests that the ethical promise of settlement derives from the potential of the subject's interests …

Spinal Matrix Metalloproteinase 8 Regulates Pain After Peripheral Trauma, Maral Tajerian, J. David Clark

Publications and Research

It is well documented that pain chronification requires a host of plastic mechanisms at the spinal cord (SC) level, including alterations in neuronal and glial structure and function. Such cellular plasticity necessitates the existence of a plastic extracellular matrix(ECM). Here, we describe a key role for ECM remodeling in the regulation of chronic pain following peripheral injury. Three weeks following tibia fracture in mice, we show increased levels of MMP8 in the SC. Furthermore, we show that the pharmacological or genetic downregulation of MMP8 ameliorates the pain phenotype observed after injury. These results delineate an extracellular mechanism for pain chronification, …

The Feres Doctrine: A Comprehensive Legal Analysis, Dwight D. Stirling

Theses and Dissertations

This study examines the Feres doctrine, the judicial policy that immunizes service members from civil liability when causing harm to each other. Created by the Supreme Court nearly 70 years ago, the insulation from judicial review is practically absolute, applying to nearly every variety of intra-military harm, from a negligent delivery that leaders to the mother's death to intentional, abhorrent wrongdoing such as sexual assault. The doctrine bars service member suits across the board. The study traces the doctrine's theoretical and philosophical roots, assessing its historical development and documenting its current state. A comprehensive legal analysis, not only is every …

Innovative Instruction: Learning In Blended Human Anatomy Education, Mia Summer Nobles

Theses and Dissertations

Despite the robust literature surrounding the benefits of blended learning including improved student learning and positive student perceptions of learning (Bishop & Verleger, 2013; O'Flaherty & Phillips, 2015), simply rearranging the structure of activities or incorporating technology does not ensure a more meaningful learning experience (Duffy & McDonald, 2008; Gopal et al., 2006). There exists a danger of educators attempting the translation of blended learning without thoroughly understanding how it works (Ash, 2012). Considering the definition of blended learning as the "organic integration of thoughtfully selected and complementary F2F and online approaches and technologies" Garrison & Vaughan, 2008, p. 148), …

Understanding Aquarium Culture In The Context Of Strategic Change, Katie E. Mcconnell

Theses and Dissertations

This study examined the relationship between organizational culture and strategic change. Specifically, how leaders can identify a preferred culture to support a new vision and create an action plan for shifting the existing to the preferred culture. Two frameworks were used: The Competing Values Culture Framework and Appreciative Inquiry. The combination of these models can be a helpful tool to create practical knowledge about culture and provide action plans to support the preferred state. Leaders indicated a 52% decrease in hierarchy and a 120% increase in adhocracy culture would effectively support the new vision. These results mapped to literature findings …

Law Of Laughter (Lol), Yakov Smirnoff

Theses and Dissertations

This dissertation derived from recognition that in the beginning of a romantic relationship people experience and remember a lot of laughter. If relationship ends there isn't any laughter. It has been proven by research that in the honeymoon stage hormones secreted in our body enhance our ability to meet each other's needs and provide a sense of well being to achieve the desired result, which would be procreation. Once that stage of the relationship is completed, Mother Nature stops supplying hormones like dopamine, oxytocin, and serotonin to help us in the process of creating happy and fun-filled relationships. The purpose …

Advocacy Education For Psychology Graduate Students: A Curriculum For Professional Advocacy In Psychology, Tracey Wheeler

Theses and Dissertations

The aim of this dissertation was to synthesize information from the empirical literature, psychology professional organizations and other relevant sources into a graduate-level curriculum for education in professional advocacy for psychology. Professional advocacy practices, such as public policy and legislative involvement, are necessary to protect and grow psychology's place in the healthcare system, funding for psychological research and access to services for the public. Remarkably low involvement by psychologists in advocacy is theorized to be due to a lack of training, awareness and professional values related to advocacy. This dissertation proposes that graduate school education is an optimal and effective …

The Plantation Pull: Modernities And Genre In The Anglo-Hispanic-Dutch Caribbean-Atlantic, 1831-1935, Natalie Magdalena Aikens

Electronic Theses and Dissertations

Using Benedict Anderson’s Imagined Communities, “The Plantation Pull: Modernities and Genre in the Anglo-Hispanic-Dutch Caribbean-Atlantic, 1831-1935” contrasts the idea of homogeneous national ideals with depictions in literature of stratified geopolitical regions deeply divided by issues of race, ethnicity, socioeconomic status matching those ambivalent spaces described by Homi Bhabha in Nation and Narration. The project demonstrates in literature the way that the values of the capitalist plantation machine based around mechanization and modernization, what I term “the plantation pull,” nevertheless thwarts one of the major iterations of modernity in the nineteenth century: nation formation. The “plantation pull” …

Households And Changing Use Of Space At The Transitional Early Mississippian Austin Site, Benjamin Garrett Davis

Electronic Theses and Dissertations

The Austin Site (22TU549) is a village site located in Tunica County, Mississippi dating to approximately A.D. 1150-1350, along the transition from the Terminal Late Woodland to the Mississippian period. While Elizabeth Hunt’s (2017) masters thesis concluded that the ceramics at Austin emphasized a Late Woodland persistence, the architecture and use of space at the site had yet to be analyzed. This study examines this architecture and use of space over time at Austin to determine if they display evidence of increasing institutionalized inequality. This included creating a map of Austin based on John Connaway’s original excavation notes, and then …

Nanosuspension Based Electrolyte Sensitive In Situ Gel For Topical Ocular Delivery Of Natamycin, Poorva H. Joshi

Electronic Theses and Dissertations

Natamycin (NT) is a commercially available antifungal drug used for the treatment of fungal keratitis, an infection which affects the clear corneal surface and its associated layers. Currently, NT is available commercially as a 5% w/v ophthalmic suspension, to be administered topically. The main objective of the present investigation was to develop and evaluate the natamycin loaded nanosuspension (NT-NS), as well as it’s corresponding in-situ gel (NT-NS-GG), for the treatment of fungal keratitis. NT-NS was prepared using homogenization technique and optimized based on size, PDI, zeta potential (ZP), assay and process stability. Further, optimized NT-NS was modified into an in-situ …

Ecological Factors Associated With Habitat Use Of Baird’S Tapirs (Tapirus Bairdii), Victoria Diane Monette

Electronic Theses and Dissertations

The Baird’s tapir, the largest terrestrial mammal in Central America and a crucial seed disperser and ecosystem engineer, has experienced a 50% loss of habitat in its geographic range during the past 30 years. Efforts to conserve this species need to consider factors that have contributed to its endangerment, such as how human presence and human-mediated habitat change may influence tapir behavior, body condition, and disease susceptibility. In this thesis I had two goals: 1) understand how human disturbance affects tapir activity patterns, and 2) noninvasively determine the association of disturbance with tapir health. I first compared the activity patterns …

Formulation Strategies To Address Physiological And Anatomical Constraints For Improved Topical Ocular Drug Delivery, Karthik Yadav Janga

Electronic Theses and Dissertations

Delivery of therapeutic molecules to the eye has been a complicated task owing to its anatomical and physiological barriers. This dissertation delineates several formulation strategies to circumvent dynamic and static constraints hampering the ocular drug delivery. Advanced vesicular systems, bilosomes and transfersomes, were prepared to improve the ocular pharmacotherapy of natamycin (NT). NT bilosomes and transfersomes were further loaded into in situ gel systems which transformed immediately to hydrogel, in the simulated tear fluid, with good viscoelastic and mucoadhesive properties.In vitro corneal transport studies confirmed the superior permeability characteristics of NT from the optimized bilosome and transfersome in situ gel …

Rambling Blues: Mapping Contemporary North American Blues Literature, Josh-Wade Ferguson

Electronic Theses and Dissertations

“Rambling Blues: Mapping Contemporary North American Blues Literature” revises the methodological assumptions that have underwritten our understanding of blues literature and the politics of race and region that surround it. Where previous commentators have defined blues literature primarily through its formal and thematic connections with blues music and with the sociohistorical contours of black southern life more generally this dissertation expands the boundaries of how we conceive blues literature by examining Langston Hughes’ poems “The Weary Blues” (1925) and “Po Boy Blues” (1926) August Wilson’s Ma Rainey’s Black Bottom (1984) Jesmyn Ward’s Salvage the Bones (2011) James Hannaham’s Delicious Foods …

Decolonial Resistance In Latinx Writings From Peru To The United States: A Portfolio, Isabel Norwood

Electronic Theses and Dissertations

This collection begins with the premise that colonial relationships manifest in ways beyond exploitation of one nation by another. It relies on the decolonial theory of Walter D. Mignolo in its assumption that imbalances of power in the realms of race gender sexuality and class are fundamentally colonial. With this more expansive understanding of coloniality in mind I examine resistance to colonial exploitation in a range of texts from across the Americas. The first essay in this collection explores the role of the guinea pig in Andean food culture arguing that the continued consumption of guinea pig represents a form …

Subverting The Patriarchal Panopticon: Challenges To Eugenics Rhetoric In The Novels Of Mccullers And Welty, Regina Marie Young

Electronic Theses and Dissertations

My thesis takes into consideration the scope of eugenics ideologies and their influence on literature specifically two mid-twentieth century authors from the U.S. South Carson McCullers and Eudora Welty. I contend that both writers engage with eugenics rhetoric challenging and subverting the prevailing ideology of the day albeit in differing ways. McCullers and Welty address different facets of eugenics rhetoric in their novels— namely the nature of “defect” and the criteria for “fitness” for “citizenship.” This thesis interrogates the ways in which these writers develop rhetorical strategies for resisting eugenics ideologies in their respective novels Reflections in a Golden Eye …

Diversification In The Neotropics – Evolution And Population Genetics Of The Armored Catfish Hypancistrus Sp. From The Xingu River, Marcella Goncalves Santos

Electronic Theses and Dissertations

The Xingu River, one of the largest tributaries of the Amazon River, is currently in peril due to the recent construction of hydroelectric dams, but little is known about the numerous fish species it supports. This dissertation focuses on three pleco catfish species belonging to the genus Hypancistrus from the Xingu River with partially overlapping distributions: H. zebra, H. sp. (L174), and H. sp. (L66/333). Chapter 1 is a bibliographic review of Amazonian freshwater fish diversity, with the goal of discussing the hypotheses of speciation mechanisms that can be tested in this system, including the relative importance of ecological adaptation …