Digital Commons Network^™

Synthesis, Characterization, And Applications Of Ammonium/Phosphonium Chitosan Derivatives For Investigative Dna Interactions, Quinton Kirk Wyatt

Graduate Theses/Dissertations

Chitosan is examined as a non-viral vector capable of efficient gene delivery due to its favorable properties such as biodegradability, biocompatibility, and is non-toxic to mammalian cells. Cell modifications and vector permeability are enhanced in non-viral vectors that incorporate electrostatic interactions. This work synthesizes cationic (N⁺, P⁺) chitosan derivatives, to enhance electrostatic interactions towards nucleotides, using amide coupling agents EDC and HOBt. The final chitosan derivatives will bear cationic functional groups (N⁺, P⁺) with various alkyl groups (Me, Et, Pr, Ph) to examine their affinities to nucleotides and antifungal properties. mPEG grafted …

Development Of Rapid, Homogeneous Assay For Investigating Isopeptide Bond Formation Using Fluorescence Polarization/Depolarization Measurements, Samuel Patricc Kasson

Graduate Theses/Dissertations

Autocatalytic intramolecular isopeptide bonds have been found in nature in certain gram-positive bacterial pilus structures. Recently, splitting of these domains that are capable of autocatalytic intramolecular isopeptide bond formation have been applied to create stable, selective, bio-orthogonal Catcher/Tag systems. The CnaB2 domain found in the FbaB pilus structure of Streptococcus pyogenes, has yielded the Catcher/Tag, Protein/Peptide systems termed SpyCatchter and SpyTag. Recent study has focused on tag optimization, stability and bio-orthogonality evaluation, along with applications in bioconjugation. I have recombinantly expressed SpyCatcher and SpyTag-fused proteins in E.coli, and conjugated them to fluorescent probes in order for use in fluorescence polarization/depolarization …

Effects Of Neem On Plasmatocyte Counts In Galleria Mellonella Larvae, Michael Anthony Fenske

Graduate Theses/Dissertations

The neem tree (Azadirachta indica) has been called the tree of the 21^st century, due to its many beneficial properties. This research studies the effects of neem products on plasmatocytes of Galleria mellonella larvae challenged by injections of Sumi ink. Counts of circulating plasmatocytes increased in response to the ink within 4 hours. Concurrent injections of neem essential oil reversed this process in a dose-dependent manner. Methanolic extracts of neem essential oil had similar effects on counts of circulating plasmatocytes mobilized by ink injections. Aqueous extracts from neem bark did not have effects on plasmatocyte counts. Comparative …

Immune Function And Metabolism Of Hibernating North American Bats With White-Nose Syndrome, Briana Nicole Anderson

Graduate Theses/Dissertations

White-nose syndrome (WNS) causes substantial mortality in certain species of hibernating North American bats. The responsible agent is Pseudogymnoascus destructans (Pd), a fungus which causes physiological complications such as increased arousals and energy depletion during the hibernation season. Tricolored bats (Perimyotis subflavus) and northern long-eared bats (Myotis septentrionalis) suffer extensive WNS mortality, while gray bats (Myotis grisescens) and big brown bats (Eptesicus fuscus) are infected, but mortality is rarely observed. It is hypothesized that there is a difference in immune responses and/or hibernation metabolism between these bat species, resulting in this …

The Identification Of Interspecific Hybrids Between Jaeger 70 X Vignoles Grapes Using Ssr Markers, Carl William Knuckles Iv

Graduate Theses/Dissertations

Jaeger 70 cultivar is an interspecific hybrid of Vitis linececumii x Vitis rupestris, with an interesting past. It was created by Hermann Jaeger of Neosho, MO, who is most notable for saving the French wine industry by developing phylloxera resistant rootstocks in the mid-1800s. Traditionally the Jaeger 70 variety has been grafted to French wine grape cultivars to impart disease resistance and cold hardiness while retaining desirable wine characteristics. One interesting characteristic of Jaeger 70 is that it is a female-only plant, allowing for many interesting areas of study. Vitis interspecific hybrid Vignoles, is a cold hardy cultivar of largely …

Nutrient Transport And Storage In A Karst Spring-Reservoir System During Baseflow, Missouri Ozarks, Heather A. Moule

Graduate Theses/Dissertations

Urban and agricultural land uses are important sources of nitrogen and phosphorus which, if in excess, can cause eutrophication in lakes and rivers. There have been few studies of nutrient transport and storage in karst spring and reservoir connected stream systems draining the Missouri Ozarks. This study aims to link the baseflow nutrient contributions of Sanders Spring to downstream reservoir outflow and the Headwaters South Dry Sac River Watershed in Springfield, Missouri. Water samples were collected seasonally and analyzed for total nitrogen (TN), total phosphorus (TP), and several other water quality parameters. Discharge was also monitored to calculate water and …

Learning Chemistry In English: Challenges Faced By Non-Native English Speakers, Arwa A. Alshehri

Graduate Theses/Dissertations

Every year many international students come to study in the United States. In addition to all the challenges associated with coming to a new country, non-native English speakers (NNES) face the additional challenge of having to learn their content in English. Therefore, in this project, I recruited NNES participants from general chemistry courses since preliminary inquiries indicated that they have the largest enrollments. Because there were relatively few students at MSU who are non-native English speakers studying chemistry, I used a case study design to be able to extract generalizations from an in-depth study of a few individuals. The aim …

(Don’T) Stand By Me: Social Regulation Of Response To Threat In Interracial Dyads, Carmelita Sharonique Foster

Dissertations

A number of factors contribute to retention among college students, including social support and feelings of belonging (Rayle & Chung, 2007). Although college campuses are among the most racially diverse settings in our segregated society (Fischer, 2011), students differ in their perception of the racial climate on campus (Chavous, 2005). Social Baseline Theory (SBT) proposes that human functioning under stress is dependent on adequate social support and that, at baseline, biological systems are adapted to operate interdependently rather than independently (Hughes, Crowell, Uyeji, & Coan, 2012). Specifically, the substantial empirical support for SBT shows that stressful situations are less toxic …

Screen Printed Moisture Sensor On Barrier Coated Sbs Board: The Characterizations Of The Hemicellulose-Based Biofilms And Their Applications For Smart Packaging, Ruoxi Ma

Dissertations

Fiber-based packaging materials have many advantages over their petroleum-based plastic competitors, such as sustainability, recyclability and stiffness/weight ratio. However, the poor barrier properties and sensitivity towards moisture are the main challenges for their extended use. Therefore, there has been a need to provide a biodegradable and biocompatible biopolymer packaging material with improved barrier properties for food and pharmaceutical packaging. Also, the moisture loss/gain problem causes a lot of waste every year. Therefore, there is a need of better tracking and sensing methods attached to the package in order to control the moisture level along the supply chain. To accomplish these …

Assessment Of Melanocyte-Specific Primary And Memory Autoimmune Responses In Vitiligo-Prone Smyth And Vitiligo-Susceptible, Non-Expressing Brown Line Chickens, Daniel Morales Falcon

Graduate Theses and Dissertations

Vitiligo is an acquired de-pigmentation disorder characterized by the post-natal loss of epidermal melanocytes (pigment-producing cells) resulting in the appearance of white patches in the skin. The Smyth line of chicken is the only model for vitiligo that shares all the characteristics of the human condition including: spontaneous post-natal loss of melanocytes, interactions between genetic, environmental and immunological factors and associations with other autoimmune diseases. In addition, an avian model for vitiligo has the added benefit of an easily accessible target tissue (a growing feather) that allows for the repeated sampling of an individual and thus the continuous monitoring of …

Sex Differences In Poisonings Among Older Adults: An Analysis Of The Toxicology Investigators Consortium (Toxic) Registry, 2010 To 2016., Gillian A. Beauchamp Md, Jennifer L. Carey Md, Tyler Adams Bs, Amy Wier, Manuel F. Colón Bs, Matthew D. Cook Do, Robert D. Cannon Do, Kenneth D. Katz Md, Marna R. Greenberg Do, Mph, Facep

Department of Emergency Medicine

PURPOSE: Adults aged >65 years are susceptible to intentional and unintentional poisoning, with contributing factors that include polypharmacy, comorbidity, susceptibility to medication error, and gaps in research. Although toxicologists are often tasked with managing and preventing poisoning among older adults, little is known about sex differences in these poisonings. The aim of this study was to review sex differences in poisonings among older adults managed at the bedside by medical toxicologists.

METHODS: All case subjects aged >65 years in the Toxicology Investigators Consortium (ToxIC) registry between January 2010 and December 2016 were reviewed. Data included reasons for exposure and consultation, …

Evaporation Depth Controls The Relationship Between Soil Water Mobility And Soil Water Isotopic Composition, John Byars Shuler

Boise State University Theses and Dissertations

Recent studies of plant water uptake assume that soil water isotopic composition can be used to infer soil water mobility. However, the strength of the relationship between mobility and isotopic composition remains poorly constrained. In addition, many ecohydrologic investigations are restricted by low sampling frequencies and insufficient soil moisture and matric potential data to support assumptions of soil water mobility. We sampled bulk soil water every 14 to 21 days in hillslope and riparian profiles during the 2016 and 2017 growing seasons in a semi-arid watershed outside Boise, ID. We collected twig samples of four tree and shrub species concurrently. …

Utilizing Satellite Fusion Methods To Assess Vegetation Phenology In A Semi-Arid Ecosystem, Megan Gallagher

Boise State University Theses and Dissertations

Dryland ecosystems cover over 40% of the Earth’s surface, and are highly heterogeneous systems dependent upon rainfall and temperature. Climate change and anthropogenic activities have caused considerable shifts in vegetation and fire regimes, leading to desertification, habitat loss, and the spread of invasive species. Modern public satellite imagery is unable to detect fine temporal and spatial changes that occur in drylands. These ecosystems can have rapid phenological changes, and the heterogeneity of the ground cover is unable to be identified at course pixel sizes (e.g. 250 m). We develop a system that uses data from multiple satellites to model finer …

Synchronous Online Peer Tutoring Via Video Conferencing Technology: An Exploratory Case Study, Christopher David Smith

Boise State University Theses and Dissertations

The objective of this study was to analyze the lived experiences of peer tutors who provide synchronous online tutoring services, at a large, accredited, public, four-year university located in the Middle Atlantic Region of the United States. An exploratory qualitative case study approach was used to conduct this study. The case chosen for this study consisted of a single holistic case that was both descriptive and intrinsic. Participants of the study consisted of students who were hired by the chosen university to serve as peer tutors as well as students that utilize the online peer tutoring service. Data collection and …

Evaluation Of Cardiomems Heart Failure Device, Danielle Moe, Msn, Rn

Doctor of Nursing Practice Projects

Background: Heart failure (HF) is a chronic condition where the heart cannot pump enough blood to adequately oxygenate cells. The CardioMEMS HF device is implanted into the pulmonary artery (PA) allowing practitioners to monitor pressures remotely and prescribe interventions. Objectives: The primary purpose of this project was to determine if CardioMEMS is an effective intervention in the reduction of hospital admissions, emergency department (ED), and clinic visits for HF patients. The secondary purpose was to determine if the quality of life (QOL) and exercise tolerance was improved post-implant. Methods: The author conducted a retrospective chart review by accessing previously compiled …

Shame, Aggression, And Self-Compassion In At-Risk Adolescents, Sara Ashline Hofmann

Dissertations

Prior research has identified a strong link between experiences of shame and aggressive behavior in at-risk and offender populations but the mechanisms of this relationship are unclear. One potential interrupter of this relationship is self-compassion, a teachable emotional regulation skill. The purpose of this study was to investigate whether nonoffender at-risk youth differed significantly in levels of self-compassion, shame, and aggression from nonviolent offender and violent offender youth with the goal of evaluating these relationships to aid in the future development of more tailored and effective interventions for court-involved youth. One hundred and six at-risk adolescents in the Rocky Mountain …

From Marriage To Households: Towards Equal Treatment Of Intimate Forms Of Life, Deborah Zalesne, Adam Dexter

Buffalo Law Review

Law and attitudes around marriage have changed drastically in our own history and are widely different across cultures. Same-sex marriage is now legal, polyamorous relationships are on the rise, and, as an empirical matter, marriage serves a different purpose than it did as little as forty years ago -- marriage is no longer a prerequisite for sexual intimacy, cohabitation, or parenthood. There are no essential elements to a definition of marriage to which the state can appeal without arbitrarily restricting citizens’ possibilities with respect to their most intimate relationships. Therefore, because any state-sanctioned version of marriage will be arbitrary, the …

An Investigation Of The Required Mr Bone Attenuation Correction For Quantitative Whole-Body Pet/Mr Imaging Using Clinical Naf Pet/Ct Studies, Hua A. Ai, Osama Mawlawi, Jason Stafford, James Bankson Phd, Yiping Shao, Michel Guindani, Richard E. Wendt Phd

Imaging Physics Journal Articles

Ai, H. , Mawlawi, O. , Stafford, R. , Bankson, J. , Shao, Y. , Guindani, M. and Wendt III, R. (2018) An Investigation of the Required MR Bone Attenuation Correction for Quantitative Whole-Body PET/MR Imaging Using Clinical NaF PET/CT Studies. International Journal of Medical Physics, Clinical Engineering and Radiation Oncology, 7, 273-295. doi: 10.4236/ijmpcero.2018.73023.

Estimating The Post-Release Mortality Of Atlantic Cod (Gadus Morhua) In The Southern Gulf Of Maine Commercial Lobster Fishery, Brett Sweezey

All Theses And Dissertations

Atlantic cod, Gadus morhua, in the Gulf of Maine has experienced heightened fishing-induced mortality since the 1990s, leading to the lowest population abundances in recorded history. Unaccounted discard mortalities within Gulf of Maine commercial fisheries may be impairing recovery efforts in this and other cod populations. With over 4 million actively fished lobster traps, the Maine commercial lobster fishery has been suggested to be a significant contributor to cod mortality rates within this region. Therefore, the discard mortality rate of Atlantic cod captured by commercial lobster gear was examined to assess its potential influence on recovery efforts using acoustic …

An Electromyography Study Of Muscular Endurance During The Posterior Shoulder Endurance Test, Neil A. Evans, Emily Dressler, Timothy L. Uhl

Physical Therapy Faculty Publications

The primary purpose was to determine if there is a difference between the median frequency slopes of 5 posterior shoulder muscles during the initial portion of the Posterior Shoulder Endurance Test (PSET) at the 90° and 135° shoulder abduction positions. Fifty-five healthy volunteers (31 females) participated. The median frequency of the posterior deltoid (PD), upper trapezius (UT), middle trapezius (MT), lower trapezius (LT), and infraspinatus (INF) was measured during the PSET at 90° and 135° of shoulder abduction. External torque of 13 ± 1 Nm was used for females and 21 ± 1 Nm for males. A fixed effect multi-variable …

Development To A Flexible Surface Acoustic Wave Sensor For Strain Sensing, Neha Vedavathi Ramachandran

Doctoral Dissertations and Master's Theses

Surface acoustic wave (SAW) sensors have increasing demand in structural health monitoring due to their passive, variable bandwidth, reliable life-cycle, high accuracy, small size, and light nature. SAW sensors can not only provide the static states of the structural system, such as temperature and pressure but also enable continuous real-time monitoring of dynamic states including strains. SAW sensors are generally fabricated with a rigid piezoceramic substrate, incapable of adapting to highly curved surfaces or flexible objects. The ongoing demands of sensor adaptability with flexible substrates, which is also capable of wireless monitoring, is the basis of the research that can …

Fgf21 Increases Water Intake, Urine Output And Blood Pressure In Rats, Tod Turner, Xian Chen, Matthew Zahner, Alan Opsahl, George Demarco, Magalie Boucher, Bryan Goodwin, Mylène Perreault

ETSU Faculty Works

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Fibroblast growth factor 21 (FGF21) is a hormone secreted by the liver in response to metabolic stress. In addition to its well-characterized effects on energy homeostasis, FGF21 has been shown to increase water intake in animals. In this study, we sought to further explore the effects of FGF21 on fluid homeostasis in rats. A single dose of a long-acting FGF21 analog, PF-05231023, significantly increased water consumption, …

The Emperor’S New Clothes: The Variety Of Stakeholders In Climate Change Regulation Assuming The Mantle Of Federal And International Authority, Linda A. Malone

Faculty Publications

In June 2017, President Donald Trump announced the United States would be withdrawing from the Paris Climate Accord. President Trump believes the United States should be more focused on its economic wellbeing than on environmental concerns. Since being elected, President Trump has, with the help of the Environmental Protection Agency, been rolling back, or attempting to roll back, major climate change regulations. However, this Article points out that due to factors such as international law, the United States Constitution, and the Administrative Procedure Act, one cannotjust simply withdraw from an international agreement, such as the Paris Accord, or take back …

Impact Of Reproductive Mode On Skeletal Development In A Reproductively Bimodal Squamate Species, Amanda Tedder

Electronic Theses and Dissertations

Viviparity has evolved multiple times within squamates. Eggshells are reduced in viviparous forms, which reduces calcium available to embryos during development. This study tested the hypothesis that reduced calcium impacts neonates of viviparous forms. Developmental series from oviparous and viviparous populations of Zootoca vivipara (reproductively bimodal) were cleared & stained to reveal bone development. Photographs (high magnification, calibrated to size) were used to obtain measurements of lengths of the body, humerus, femur, skull and lower jaw, and of ossified portions of limb bones. Percent ossification was scored for targeted skull bones. Results were analyzed using general linear models and revealed …

Species Delimitation Predictions Using Mitochondrial And Nuclear Dna Sequences From The Heteromys Pictus-Spectabilis Species Complex, Joanna Rosa Bateman

Theses and Dissertations

Heteromys pictus-spectabilis is a species complex within the subfamily Heteromyinae (Family: Heteromyidae) that is distributed along the western and southern Mexican coast and surrounding environments. Currently, the species complex is accepted as being 2 separate species (H. pictus and H. spectabilis), but this also renders H. pictus paraphyletic. Therefore, the species complex requires re-evaluation in order to resolve the paraphyly. Mitochondrial DNA sequences from a previously existing ~720 specimen database compiled by Victoria Vance were used in conjunction with new nuclear DNA sequences sequenced for the purpose of this study to generate multiple phylogenetic trees via the software programs RAxML, …

The Responses Of Ants And Other Invertebrates To Fire And Rodent Activity In North American Deserts, Joshua David Day

Theses and Dissertations

Human activities are increasing the size, frequency and severity of disturbance across earth’s ecosystems including deserts. Exotic annual grasses have altered fire regimes by increasing the size, frequency, and severity of fires in these systems. Invertebrates make up a large proportion of ecosystem diversity, provide a wide range of ecosystem functions, and are good indicators of ecosystem function and resilience. Ants are particularly good indicators of ecosystem stability. The ability of rodents to modify plant community structure post-fire, could result in rodent communities having important indirect effects on invertebrate communities. In chapter 1 we report changes in ant forager abundance …

Fluorescent Labeling Of Antibiotic Resistant Bacteria Model Dna, Janice Darko

Theses and Dissertations

Global threats to treatment of bacterial infections due to antibiotic resistance (AR) have been on the rise in recent years. Current diagnostic tests identify bacteria by using blood culture, which takes more than 24 hours. This study focuses on the fluorescent labeling of DNA derived from bacterial AR genes (KPC & VIM) and other model DNAs using oligreen dye (OG) and molecular beacons (MB). A NanoDrop 3300 fluorospectrometer was used to take fluorescence measurements. Linear dynamic range and labeling efficiency were dependent on the following optimized conditions: dilution factor of OG (200 fold), buffer (20 mM Tris HCl, pH 8), …

The Effects Of Ice And Tens Combination Treatment On Knee And Hip Joint Neuromechanics In Individuals With Experimentally Induced Knee Pain During Running, Sunku Kwon

Theses and Dissertations

Context: Knee injury is a common problem for runners. Knee pain is a common symptom in knee injury and is associated with alterations in knee and hip muscle activation and hip joint angles. Relieving pain through intervention may help to restore neuromuscular function. Objective: To examine the effects of ice and transcutaneous electrical nerve stimulation (TENS) combination treatment on perceived knee pain, hip frontal plane angle, and muscle activation during running in individuals with experimental knee pain (EKP). Design: Crossover. Setting: Laboratory. Subjects: 19 participants (11 males and 8 females, 23.2 ± 1.9 y, 176 …

Rna Binding Proteins Co-Localize With Small Tau Inclusions In Tauopathy, Brandon F. Maziuk, Daniel J. Apicco, Anna Lourdes Cruz, Lulu Jiang, Peter E. A. Ash, Edroaldo Lummertz De Rocha, Cheng Zhang, Wai Haung Yu, John Leszyk, Jose F. Abisambra, Hu Li, Benjamin Wolozin

Sanders-Brown Center on Aging Faculty Publications

The development of insoluble, intracellular neurofibrillary tangles composed of the microtubule-associated protein tau is a defining feature of tauopathies, including Alzheimer’s disease (AD). Accumulating evidence suggests that tau pathology co-localizes with RNA binding proteins (RBPs) that are known markers for stress granules (SGs). Here we used proteomics to determine how the network of tau binding proteins changes with disease in the rTg4510 mouse, and then followed up with immunohistochemistry to identify RNA binding proteins that co-localize with tau pathology. The tau interactome networks revealed striking disease-related changes in interactions between tau and a multiple RBPs, and biochemical fractionation studies demonstrated …

When Beauty Is The Beast: The Effects Of Beauty Propaganda On Female Consumers, Savannah Greenfield

Theses/Capstones/Creative Projects

Women are faced with a constant barrage of advertisements every day and the subliminal messages that come with this visual assault. These messages are influenced by years of sexism and oversexualization in the media, and shape the actions of female consumers. This investigation reviews three existing studies that demonstrate the negative effects of beauty advertisements on women. These effects are then studied through the lens of female consumer behavior to evaluate women’s motivations in continuing to buy beauty products. A link between negative effects and consumer motivations is discussed to reveal the harsh grip of sexism on the beauty and …