Digital Commons Network^™

Intra- And Inter-Molecular Signaling In A Cardiac Connexin: Role Of Cytoplasmic Domain Dimerization And Phosphorylation, Andrew J. Trease

Theses & Dissertations

As critical mediators of cell-to-cell communication, gap junctions (GJs) are comprised of membrane channels that directly link the cytoplasm of adjacent coupled cells thereby allowing for the passage of ions, small metabolites, and secondary messengers. Each channel is formed by the apposition of two connexons from adjacent cells, each composed of six connexin (Cx) proteins. Each GJ channel functions to promote signal propagation and synchronization of cells and tissues in organs. Furthermore, GJs are essential for proper propagation of cardiac action potentials from one cell to the next, leading to the coordinated contraction and relaxation of heart muscle powering circulation. …

Functional And Mechanical Role Of Splice Variant Of Mucin4 (Muc4/X) And Trefoil Factors In Pancreatic Cancer Pathogenesis, Rahat Jahan

Theses & Dissertations

Pancreatic Cancer (PC) is one of the vicious cancers as it ranks third in the race of leading cause of cancer-related death. Lack of early diagnostic marker, poor understanding of molecular mechanism of the disease and failure to conventional chemotherapy makes this disease dreadful.

Mucin 4 (MUC4), a high molecular weight glycoprotein is one of the top differentially expressed molecules in PC while not expressed in normal pancreas. Accumulating evidence from our lab suggested its tumorigenic role in PC by increasing cell proliferation, invasion, chemotherapy resistance, tumor growth, and metastasis. Previously, our lab and other has identified 24 different splice …

Modulating Hallmarks Of Cholangiocarcinoma, Cody Wehrkamp

Theses & Dissertations

How are cholangiocarcinoma cells different from non-malignant cholangiocytes?

All of the hallmarks of cancer apply- those reported in this dissertation include cell proliferation, migration, apoptosis, and evasion of growth suppression. My studies began with testing how apoptosis might be regulated through embelin, a small molecule reported to sensitize cells to apoptosis by blocking XIAP. My data however revealed that embelin reduced the proliferative capacity in cholangiocarcinoma cells, but did not increase cell death. Malignant cells exhibit dysregulation of microRNA processing and expression. Hence, my studies seeking ways that cholangiocarcinoma eludes apoptosis transitioned to the oncomiR miR-106b, which is overexpressed in …

From La Costa Chica To Pasadena: Transnational Racial Politics Of Afro-Mexicans, Antonio Rodriguez-Santiago

Master's Theses

The ethnographic research in this thesis focuses on one group of Afro-Mexicans who migrated to Pasadena, California, from Mexico’s Costa Chica, on the Pacific Coast of Oaxaca and Guerrero, the area where a social movement for Afro-Mexican recognition and collective rights was founded in 1997. This study, using a framework of racial theory, examines the process of racial formation in a transnational setting, which has made this group different in many ways from the Black residents who remain in the Costa Chica. The study also considers how mestizaje, the Mexican national racial ideology that endorses a mixed-race society while …

Electropolymerization Of Polyaniline In The Presence Of Ferricyanide: Electrochemical, Spectroscopic And Gravimetric Studies, Lucas Marinelli

Master's Theses

In this work, ferricyanide-doped polyaniline (PANI) is electropolymerized by constant current and cyclic voltammetry (CV) measurements with simultaneous monitoring of mass changes using electrochemical quartz crystal microbalance (eQCM). Raman spectroscopy, Fourier transform infrared spectroscopy and CV measurements confirm the incorporation of the ferricyanide into the film and indicate modified electrical conductivity. UV-Vis absorbance spectroscopy and Raman spectroscopy confirm the conducting ‘emeraldine-salt’ state and confirm the presence of polarons, i.e. charge carriers.

The addition of ferricyanide during electropolymerization of the PANI film results in an increase of polymerization rate as confirmed using eQCM. A decrease in counter ion ingress/egress and an …

Afterschool Educators' Teaching Practices Through Tinkering: Nurturing Student Collaboration, Engagement, And Development Of Self-Confidence, Lianna Kali

Master's Theses

Since the early 2000s constructionist pedagogy has become an increasingly popular trend in both formal and informal science, technology, engineering, art, and math (STEAM) learning environments to support transformative educational outcomes through the making of personally meaningful objects and artifacts. But with this rise in popularity comes a challenge for educators: understanding what are the teaching practices that are most effective in a maker-centered learning environment to support student learning. Through an ethnographic case study of the teaching practices in a tinkering afterschool program, this study examines the core pedagogical facilitation moves of tinkering educators and investigates how those moves …

Detection And Melting Of Surface-Bound Dna Using A Purely Electrochemical Approach, Denny Ho

Master's Theses

In this thesis, we demonstrate an electrochemical approach for monitoring the electric-field induced melting of surface-bound double-stranded DNA. The electrochemical routine involves repeated chronoamperometry pulses to melt the duplex and square wave voltammetry to monitor the extent of melting. We utilize a scanning potential and constant potential technique to generate melting curves and access the stability and kinetics of the DNA duplexes. Our method uses a mixed monolayer of thiol-modified DNA oligomers and mercaptohexanol on gold electrodes, are subsequently incubated with target DNA covalently modified with electroactive methylene blue. Under room temperature, 10 mM Tris, and optimized electrochemical parameters, DNA …

The Best Interests Of The Child Or The State? The Rights Of The Child In Non-Lpr Cancellation Of Removal, Lizzie Bird

Master's Theses

This thesis argues that the United States is failing to fulfill its obligations under the Convention on the Rights of the Child (CRC) in its adjudication of the hardship standard in non-LPR cancellation of removal. It is well-documented that the current interpretation of the “exceptional and extremely unusual hardship” standard results in the separation of families and de facto deportation of children, many of whom are U.S. citizens. This thesis contends that this practice is not only unjust, but also unlawful.

First, it argues that the CRC in general and Article 3 (the “best interests” principle) in particular have risen …

Catalog Of Voluntary Carbon Offsets In Commercial Aviation: Understanding Roles Of Corporations And States, Shannon Hahn

Master's Theses

While the need for travel exists in the field of International Studies and in light of the very complex global climate crisis and increased international commercial air travel this paper catalogs and analyzes the trailblazing voluntary carbon offset programs of 1) Air New Zealand, 2) British Airways (IAG), 3) Cathay Pacific, 4) Emirates, 5) Lufthansa, 6) Qantas, 7) Scandinavian Airlines (SAS) and 8) United Airlines, arguing that voluntary carbon offsets as a response to the global climate crisis are ineffective and an echo to international agreements being unenforceable. The paper is hopeful for a corporate focus on technology and innovation …

Micrat: A Novel Algorithm For Inferring Gene Regulatory Networks Using Time Series Gene Expression Data, Bei Yang, Yaohui Xu, Andrew Maxwell, Wonryull Koh, Ping Gong, Chaoyang Zhang

Faculty Publications

Background: Reconstruction of gene regulatory networks (GRNs), also known as reverse engineering of GRNs, aims to infer the potential regulation relationships between genes. With the development of biotechnology, such as gene chip microarray and RNA-sequencing, the high-throughput data generated provide us with more opportunities to infer the gene-gene interaction relationships using gene expression data and hence understand the underlying mechanism of biological processes. Gene regulatory networks are known to exhibit a multiplicity of interaction mechanisms which include functional and non-functional, and linear and non-linear relationships. Meanwhile, the regulatory interactions between genes and gene products are not spontaneous since various …

Influence Of External Peer Reviewer Scores For Funding Applications On Funding Board Decisions: A Retrospective Analysis Of 1561 Reviews, Lexy Sorrell, Nicola Mcardle, Taeko Becque, Helen Payne, Beth Stuart, Sheila Turner, Jeremy C. Wyatt

Peninsula Medical School

ObjectivesTo evaluate the influence of external peer reviewer scores on the National Institute for Health Research (NIHR) research funding board decisions by the number of reviewers and type of reviewer expertise.DesignRetrospective analysis of external peer review scores for shortlisted full applications for funding (280 funding applications, 1236 individual reviewers, 1561 review scores).SettingFour applied health research funding programmes of NIHR, UK.Main outcome measuresBoard decision to fund or not fund research applications.ResultsThe mean score of reviewers predicted funding decisions better than individual reviewer scores (area under the receiver operating characteristic (ROC) curve 0.75, 95% CI 0.69 to 0.81 compared with 0.62, CI …

Transcriptome 3′End Organization By Pcf11 Links Alternative Polyadenylation To Formation And Neuronal Differentiation Of Neuroblastoma, A Poetsch, S Danckwardt

School of Biomedical Sciences

Diversification at the transcriptome 3'end is an important and evolutionarily conserved layer of gene regulation associated with differentiation and dedifferentiation processes. Here, we identify extensive transcriptome 3'end-alterations in neuroblastoma, a tumour entity with a paucity of recurrent somatic mutations and an unusually high frequency of spontaneous regression. Utilising extensive RNAi-screening we reveal the landscape and drivers of transcriptome 3'end-diversification, discovering PCF11 as critical regulator, directing alternative polyadenylation (APA) of hundreds of transcripts including a differentiation RNA-operon. PCF11 shapes inputs converging on WNT-signalling, and governs cell cycle, proliferation, apoptosis and neurodifferentiation. Postnatal PCF11 down-regulation induces a neurodifferentiation program, and low-level PCF11 …

Establishing And Evaluating Agricultural Plantings And Supplemental Cover On Reservoir Mudflats As A Means To Increase Juvenile Game Fish Abundance And Growth, Hunter R. Hatcher

Theses and Dissertations

Reservoirs throughout the country exhibit degraded shorelines and simplified littoral habitats because of aging. This study evaluated the establishment of agricultural plantings on reservoir mudflats and the effectiveness of supplemental brush pile structures in providing recruitment habitat for juvenile fish. The mudflats of Enid Reservoir, Mississippi were seeded during the winter drawdown in October of 2016 and 2017 with agricultural plantings. Monitoring of plantings found grasses performed best in terms of establishment and providing potential fish habitat. During Summer 2017 brush piles and control sites, without brush, were sampled in Enid Reservoir using rotenone to evaluate juvenile fish use. Juvenile …

Estimating Clapper Rail (Rallus Crepitans) Survivorship And Implementation Of Estimates Into Individual-Based Population Models, Jared Feura

Theses and Dissertations

Sea-level rise is a concern for the future of coastal marsh obligate species such as the Clapper Rail (Rallus crepitans). Clapper Rails possess the potential to indicate changes to marsh ecological state due to population variation related to habitat features exhibited in previous study. Estimates for Clapper Rail survival are among the key missing parameters to create predictive models for Clapper Rail populations. I estimated Clapper Rail survival using data collected from six automated telemetry towers located in two Mississippi marshes. Thirty adult rails were harnessed with radio transmitters around telemetry towers to provide evidence of a rail’s status, alive …

Examining The Impact Of Trauma On Reading Performance Among Elementary Students, Shelby Lynn Mcgrew

Theses and Dissertations

Beginning prior to the foundational study conducted by Felitti et al. (1998), individuals of all ages have been exposed to a variety of traumatic events that had the capacity to alter overall functioning in a variety of ways. Children and adolescents are a vulnerable subset of individuals who are often exposed to various types of trauma which has the capacity to impact academic, behavioral, and social/emotional functioning (Cohen, Berliner, & Mannarino, 2010). The adverse effects related to trauma exposure in children and adolescents have been correlated with externalizing and internalizing disorders, and decreased academic performance (American Psychiatric Association, 2013). An …

Organic And Inorganic Contaminants Removal From Water With Engineered Biochar, Narada Bombuwala Dewage

Theses and Dissertations

Water pollution represents one of the major concerns of the modern world, after scientific and industrial development that generates hazardous organic and inorganic contaminants. Biochar (BC) has gained tremendous attention in the past decade as a cheap and efficient adsorbent for organic and inorganic contaminants from aqueous solutions. BC is considered to be a low-cost alternative to activated carbon, however, BC typically suffer performance reductions due to their low surface areas and poor mechanical properties. The main objective of this work is to develop novel biochar materials by modifying the biochar surface for the removal of organic and inorganic contaminants …

Parent And Child Perceived Coping Socialization During Peer Conflict: A Qualitative Study, Janet W. Kwan

Theses and Dissertations

Despite budding research on parent-child discrepant perceptions of a child’s psychosocial functioning, the potential difference in individual perception of coping socialization is unclear. Further, literature suggests the onset of various impulse-control disorders occurs around middle childhood. Thus, the current study used a phenomenological approach and thematic analysis to examine perspectives of coping socialization, identify intended take away messages, and examine the perceived impact of inconsistent understanding of the interaction. Results indicated both parents and children use verbal and physical cues to understand one another during coping socialization and that their lab discussion was representative of their typical interactions. Themes emerged …

Microbial Succession From A Controlled Death Event Following Simulated Mass Mortality, Lindsay K. Harrison

Theses and Dissertations

An increasing trend in mass mortality events (MMEs) has been observed in recent years, leading to an increased study of these events and their causes. Still to be investigated are the immediate and long-term effects of these environmental disturbances. Microbial communities found on and within the carcass are a major contributor to decomposition. With an increased biomass from several carcasses, transfer of these microbes to secondary death events may be affected. For this project, several simulated MMEs were used in conjunction with a secondary death event to observe the effects of transfer between the microbial communities and changes in the …

Scaling Undergraduate Scientific Writing Via Prominent Feature Analysis, Katarzyna Zaruska Gallo

Theses and Dissertations

Prominent Feature Analysis (PFA) is a reliable and valid writing assessment tool, derived from the writing it is used to assess. PFA, used to assess on-demand expository essays in Grades 3-12, uncovers positive and negative characteristics of a sample. To extend PFA to a new academic level and genre, I assessed scientific writing of 208 undergraduates, identifying 35 linguistic and 20 scientific prominent features. An essay could earn up to 28 positive (24 linguistic and four scientific), and up to 27 negative marks (11 linguistic and 16 scientific). The minimum prominent features number in a paper was 3, the maximum …

Smoke Signals: New Contexts For The Emergence, Spread, And Decline Of Effigy Pipes In Southeastern North America, A.D. 1000-1600, Charles Van De Kree

Theses and Dissertations

The cultural significance of effigy pipes among southeastern groups during the Mississippian period (A.D. 1000-1600) has yet to be fully understood. Recent studies, however, have provided new archaeological contexts for framing explanations of their possible use and distribution among such groups. Apart from conjectures about their use as ceremonial objects, selection for effigy pipes in the Mississippian Southeast was directly related to fluctuating environmental and demographic conditions under which such objects were manufactured and distributed. These conditions provided the appropriate context for their emergence as costly signaling devices through which elite or special interest groups advertised fitness levels, typically expressed …

Social Inequality In The Early Bronze Age At Bab Edh-Dhra, Jordan, Natalie Patience

Theses and Dissertations

Bab edh-Dhra is the most extensively excavated cemetery from Early Bronze Age, Jordan. Despite thorough study, the social structure and existence of social inequality remain unclear. This was addressed using osteological evidence of physiological stress to compare between family tombs. In societies exhibiting social inequality, individuals of lower status experience higher levels of stress. Evidence of physiological stress (femoral length, LEH, metabolic disorders, periosteal reactions, cribra orbitalia, and porotic hyperostosis) was recorded using standard methods for 250 adults. The artifact counts in this study have been previously published. Differences in the frequency of stress indicators were compared using chi-square tests. …

Stress-Buffering Of Social Support On Depressive Problems: Moderation By Parental Depressive Problems, Erica Marie Szkody

Theses and Dissertations

The impact of parental depressive problems on children’s depressive symptoms has been widely studied. The Stress Buffering Hypothesis states that social support acts as a protective factor between the impacts of stress from negative life events on physical and psychological health. The current study examined the stress buffering hypothesis in terms of the relationship between parental depressive problems and emerging adult depressive problems. The differential buffering strength of both perceived social support and received social support between stress from negative life events and depressive problems were examined along with the moderating effects of parent and emerging adult gender. Neither perceived …

System Dynamics Model Of Necrotic Enteritis And Its Predisposing Factors In Broilers, Yu-Bin Chou

Theses and Dissertations

Necrotic enteritis (NE) caused by Clostridium perfringens type A is an important bacterial enteric disease of global broiler production. However, the dynamic interactions of NE and its predisposing factors are not fully presented by current studies. By using the System Dynamics (SD) Model, the epidemiological changes in susceptible-infected-removed models of NE and avian coccidiosis and their interactions in one or multiple grow-out cycles was established; meanwhile, the growth performance was measured by the average weights of infected and non-infected populations at harvest were estimated. The SD model provided direct and persuasive outcomes of the epidemiology and ecology of NE compared …

In Vivo Study Of The Effect Of Different Levels Of Chemical Fertilizers On The Indigotin Dye In The Indigofera Tinctoria Plant Using Raman Spectroscopy, Fatemah Alharthi

Theses and Dissertations

An impact of nitrogen, phosphorus and potassium fertilizers on the concentration changes of carotenoid pigments in the Indigofera tinctoria plant by using Raman spectroscopic techniques is studied. Three different concentration levels of the fertilizers with a normal supply as control were added to the plants at two stages. The Raman spectra were taken to determine the carotenoid concentration level changes in the plant leaves in vivo. The ring-stretching mode are the Raman spectroscopic signatures for the carotenoid pigment and its magnitude increased significantly (over 170%) for the case of phosphorus and potassium fertilizers. The effect from the nitrogen fertilizer was …

Influence Of Winter Annual Cover Crops And Insect Management Strategies On Insect Pests Of Mississippi Soybean, Daniel Adam Whalen

Theses and Dissertations

An increasing cultural practice in soybean, Glycine max (L.), production is the use of winter annual cover crops before planting. Species of grasses, legumes, and forbs are planted for many agronomic purposes during the fall months. In the spring, cover crops are killed and soybean planted into the residue. When the termination of the cover crops is delayed for longer lasting benefits, insect pest issues can arise. The movement of insect pests from cover crops to subsequent cash crops happens through a connection known as the “Green Bridge”. Pests found in cover crops such as the pea leaf weevil, Sitona …

Interaction Energies And Electronic Spectra Of Fluorene-Receptors Molecules For Carbon Dioxide Detection, Mawuli Deegbey

Theses and Dissertations

The world’s oceans absorb a significant percentage of anthropogenic carbon emissions, and CO2 levels have profound effects on the marine environment. Of primary concern is the acidification of the oceans due to dissolved CO2. The goal of this research is to design new sensing technologies for deployment in the marine environment to detect CO2 pollutant. A series of carbon dioxide (CO2) receptors that are complexed to fluorene oligomers were studied computationally. In chapter 1, an overview of CO2 chemistry and various CO2 sensors is discussed. A short overview of the method (Kohn-Sham density functional theory and time-dependent density functional theory …

Health Science Curriculum For Early Childhood: Teacher Implementation And Impact On Child Health Knowledge, Carla J. Mays

Theses and Dissertations

This dissertation contains two secondary quantitative data analyses studies. In the first, implementation of the WannaBee Healthy? Be Smart! Be Active! Be a Leader! health science curriculum was examined to expand understanding about teacher usage of an integrated health curriculum. Specifically, researchers assessed the amount of activities within each curriculum domain (i.e., books, creative expressions, language/literacy, math, science) and the number of activities within each theme of the lessons (i.e., nutrition, physical activity, sleep) utilized by participating teachers. Prior to implementation, teachers (N = 68; M age = 35.5 years old) attended a one-hour training where use of the curriculum …

Healthcare Experiences Of Transgender Mississippians: A Qualitative Study, Micah D. King

Theses and Dissertations

For this study, in-depth, semi-structured interviews were conducted with 16 transgender adults who lived, or had previously lived, in Mississippi and sought healthcare services in the state. The questions asked focused on identifying the barriers to care that these individuals faced as well as the strategies for navigating the barriers they employed. Recommendations for providers were also considered.

Removal Of Dietary Antimicrobials And Effects Of Their Replacement With Bacillus Subtilis On The Growth And Intestinal Health Of Male Broilers, Kacey O'Donnell

Theses and Dissertations

The effects of dietary antimicrobial removal and Bacillus subtilis supplementation on the growth and intestinal health of male broilers were investigated. Birds were fed either a control, antimicrobial, or a B. subtilis probiotic diet at different feeding phases. Birds were challenged with a 10 × dose of a coccidiosis vaccine. Supplementation of B. subtilis in for antimicrobials in the late grower and early finisher phases improved growth similar to birds fed antimicrobials until withdrawal, while antimicrobial removal without B. subtilis supplementation in those periods hindered growth. The improved growth suggests that the probiotic was able to alleviate the stress of …

Expression Analysis Of Micrornas And Microrna-Like Rnas In Aspergillus Flavus-Infected Aflatoxin Resistant And Susceptible Maize Inbred Lines, Amanda Benton Harper

Theses and Dissertations

Corn (Zea mays) is frequently infected by a soil fungal pathogen Aspergillus flavus. The fungus produces aflatoxins, which cause liver cancer. Maize inbred lines that are resistant to infection by A. flavus have been developed, and these inbred lines provide excellent models for studying molecular mechanisms of maize resistance to the fungus. MicroRNA-like RNAs (milRNAs) recently identified in A. flavus had been found to be correlated with aflatoxin production conditions, suggesting that the milRNAs might play a role in the regulation of aflatoxin production. In this research, small RNAs were isolated from kernels of maize (resistant Mp719 and susceptible Va35) …