Digital Commons Network^™

Evaluation Of The Performance Of Maldi-Tof Ms And Dna Sequence Analysis In The Identification Of Mycobacteria Species, Işin Akyar, Cengi̇z Çavuşoğlu, Meltem Ayaş, Süheyla Sürücüoğlu, Zeynep Arzu İlki̇, Deni̇z Ece Kaya, Yeşi̇m Beşli̇

Turkish Journal of Medical Sciences

Background/aim: Matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry is an alternative way of identifying mycobacteria via the analysis of biomolecules. It is being increasingly used in routine microbiology practice since it permits early, rapid, and cost-effective identification of pathogens of clinical importance. In this study, we aimed to evaluate the efficacy of phenotypic identification of mycobacteria by the MALDI-TOF MS MBT Mycobacteria Library (ML) 4.0 (Bruker, Daltonics) compared to standard sequence analysis. Materials and methods: A total of 155 Mycobacterium clinical and external quality control isolates, comprising nontuberculous mycobacteria (NTM) (n = 95) and the Mycobacterium tuberculosis complex (MTC) (n …

Antibody Dependent Enhancement Of Visceral Leishmaniasis, Alan K. Mcnolty

All Master's Theses

Leishmaniasis is a parasitic disease caused by protozoans of the genus Leishmania. This vector-born disease, transmitted by biting phlebotomine sandflies, typically manifests in one of three ways. The cutaneous form of the disease is characterized by localized lesions of the skin and is by far the most common manifestation. The visceral form of the disease is caused by parasitic infiltration of internal organs, particularly the spleen, liver, and bone marrow. The mucocutaneous form is caused by parasitic infection of the mucosa in the nose or mouth. While cutaneous leishmaniasis (CL) is often self-healing, visceral leishmaniasis (VL) is fatal if …

Demographic And Clinical Predictors Of Trait Impulsivity In Parkinson’S Disease Patients, Maddeson Riley, Megan Bakeberg, Michelle Byrne, Alexa Jefferson, Soumya Ghosh, Rick Stell, Frank L. Mastaglia, Dana A. Hince, Ryan S. Anderton

Health Sciences Papers and Journal Articles

Background: Impulsive behaviour has become increasingly recognised as a neuropsychiatric complication of Parkinson’s disease (PD). Thought to be a product of compromised cognitive control, the spectrum of impulsive behaviours in PD ranges from cognitive disinhibition to impulse control disorders (ICDs).

Objective: At present, there are no indicators for trait impulsivity in PD. The objective of the current study was to identify demographic and clinical predictors of susceptibility to trait impulsivity in a cohort of PD patients.

Methods: The current study assessed impulsivity using the Barratt Impulsiveness Scale 11 (BIS-11) in a cohort of 87 PD patients. General linear models (GLMs) …

Factors Contributing To Australian Adolescents’ Self-Report Of Their Motor Skill Competence, Amanda Timler, Fleur Mcintyre, Beth Hands

Health Sciences Papers and Journal Articles

An adolescents motor skill competence can affect areas such as sports participation, social activities and future academic or employment decisions. The Adolescent Motor Competence Questionnaire (AMCQ) is a 26-item questionnaire that uses a four point Likert response (never, sometimes, frequently, always) to assess motor-related activities during adolescence. This study aims to provide evidence of the construct validity using Principle Component Analysis (PCA) and to identify factors that contributed to Australian adolescent self-reported motor competence. A final aim was to determine whether individual item responses differed between males and females. The AMCQ was completed by 160 adolescents (M_age = 14.45 …

Anticorrosion Coating For Magnesium Alloys: Electrospun Superhydrophobic Polystyrene/Sio$_{2}$ Composite Fibers, Nesri̇n Horzum Polat, Özlem Kap, Ami̇r Farzaneh

Turkish Journal of Chemistry

Superhydrophobic nanocomposite coatings for magnesium surfaces with remarkable corrosion resistance were fabricated by electrospinning in the presence of fluorosilane-functionalized silica (SiO$_{2})$ nanoparticles. The effects of surface-modified silica (mod-SiO$_{2})$ nanoparticles on the superhydrophobicity and corrosion resistance of polystyrene (PS)/mod-SiO$_{2}$ fiber coatings were evaluated. The incorporation of the SiO$_{2}$ nanoparticles endows PS fibers with rough surfaces exhibiting a water contact angle (WCA) of 165$^{\circ}$. The surface wettability, corrosion resistance, and their relation to the inorganic content in the PS fibers and the contact angle of the composite coatings were explored. Analysis of the corrosion results confirmed that the PS/mod-SiO$_{2}$ coating protected the …

One-Pot Multicomponent Synthesis Of Piperidinium 3,3'-(Arylmethylene) Bis (2-Hydroxynaphthalene-1,4-Diones): Nmr Spectroscopic And X-Ray~Structure Characterization, Maryam Fatahpour, Nourollah Hazeri, Malek Taher Maghsoodlou, Fatemeh Noori Sadeh, Mojtaba Lshkari

Turkish Journal of Chemistry

A facile synthesis of piperidinium 3,3'-(arylmethylene) bis (2-hydroxynaphthalene-1,4-dione) analogs as organic salts is described by the one-pot pseudo-four-component reaction between 2-hydroxy-1,4-naphthoquinone, aromatic aldehydes, and piperidine. The single-crystal X-ray diffraction analysis of these systems confirms that the stabilized predominant interactions are N-H\... O and O-H\... O hydrogen bonds. Mild and clean reaction conditions, high atom economy, and operational simplicity of this one-pot multicomponent reaction coupled with excellent yields and no need for column chromatography have transformed this procedure to be a superior synthetic route for the efficient formation of families of naphthoquinone-derived compounds.

The Interaction Between A Zinc(Ii) Phthalocyanine Compound Bearing Octakis Phenoxyacetamide Substituents And Calf Thymus Dna, Ali̇ Arslantaş, Mehmet Sali̇h Ağirtaş

Turkish Journal of Chemistry

In this study, a zinc(II) phthalocyanine compound (PcZn) substituted with octakis phenoxyacetamide was synthesized and characterized. Calf thymus DNA (CT-DNA) was used to determine the DNA binding properties of the zinc(II) phthalocyanine compound. This interaction of CT-DNA with PcZn was investigated using electronic absorption spectra, emission spectroscopy, a thermal melting temperature study, cyclic voltammetry, and viscosity measurements in a Tris-HCl buffer solution at a pH of 7.2. The findings showed that zinc(II) phthalocyanine interacts with calf thymus DNA via partial intercalative binding mechanisms. In addition to the techniques above, a gel electrophoresis study was also carried out to verify the …

A Novel Core-Shell-Based Chromatographic Method Supported By Ratio Derivative Spectrophotometry For The Simultaneous Determination Of Perindopril, Indapamide, And Amlodipine Ternary Mixtures, Leyla Karadurmuş, Mehmet Gümüştaş, Sevi̇nç Kurbanoğlu, Bengi̇ Uslu, Si̇bel Ayşil Özkan

Turkish Journal of Chemistry

}In this work, ratio spectra of the first derivative spectrophotometric and liquid chromatographic methods have been described for the first time for the simultaneous determination of perindopril (PER), indapamide (IND), and amlodipine (AML) in dosage forms. For chromatographic separations several mobile phase compositions were tested for efficient separation with the use of a new column technology related to superficially porous particles. Optimum chromatographic separation was achieved using a Kinetex C18 column (150 $\times $ 4.6 mm I.D. 5 $\mu $m) at a flow rate of 1.5 mL min$^{-1}$. The separation was carried out at 30 $^{\circ}$C and the diode array …

Synthesis And Antimicrobial And Antioxidant Activities Of Hybrid Molecules Containing Benzotriazole And 1,2,4-Triazole, Mahesh Chand, Reena Kaushik, Subhash Chand Jain

Turkish Journal of Chemistry

Eleven novel 1,2,4-triazolylbenzotriazoles have been prepared using 1-(hydrazinylcarbonylmethyl)-1$H$-benzot- riazole (3) as a potent intermediate. Compound 3, however, was obtained from benzotriazole in two steps. All synthesized compounds were characterized by detailed spectral studies like IR, $^{1}$H NMR, $^{13}$C NMR, and mass spectrometry. All synthesized compounds were evaluated for their \textit{in vitro} antimicrobial activity against seven strains of bacteria and four strains of fungi. Compounds 13, 17, 19, 20, and 21}were found to possess antibacterial activity comparable to that of ciprofloxacin against a Klebsiella pneumoniae strain. Except for compounds2 and 13, all compounds showed good antifungal activity against an Aspergillus niger …

Voltammetric Determination Of Glycopyrrolate In A Pharmaceutical Formulation, Muneer Fadr, Abdulaziz Nabil Amro, Sami Ben Aoun, Samer Ratrout, Fadi Asfour

Turkish Journal of Chemistry

The electrochemical activity of glycopyrrolate was studied. Different voltammetric techniques were applied in this study, including cyclic voltammetry (CV), differential pulse voltammetry (DPV), and linear sweep voltammetry (LSV). Glassy carbon and platinum working electrodes were utilized. CV indicated that glycopyrrolate has a reversible redox reaction on the working electrode surface, with an anodic peak current at approximately 1.05 V and a cathodic peak at 0.80 V. Several parameters that affect the sensitivity of these methods were optimized for the quantitation of glycopyrrolate. LSV showed a better correlation coefficient than DPV did, with a value of ca. 0.9990 in the range …

Determination Of Hydrogen Peroxide With An Enzymeless Amperometric Sensor Based On Poly(Vinylferrocene)-Supported Ag Nanoparticles, Mutlu Sönmez Çelebi̇, Kübra Öztürk, Mehmet Dumangöz, Fi̇li̇z Kuralay

Turkish Journal of Chemistry

An enzymeless electrochemical sensor for detection of low amounts of H$_{2}$O$_{2}$ with the aid of Ag nanoparticles supported on conducting poly(vinylferrocene) (PVF) film was developed. Experimental results revealed that contribution of Ag nanoparticles led to remarkable improvement by means of reduction potential and reduction current. Influence of experimental parameters (i.e. polymeric film thickness, concentration of Ag precursor, immersion time in precursor solution, reduction time, and reduction potential) were investigated. The Ag/PVF-modified electrode system was characterized physically by scanning electron microscopy. The results revealed that the sensor developed was easy-to-prepare, economic, selective, and sensitive, with a fast response time of 3 …

Improving The Phylogenetic Understanding Of The Genus Juniperus, Therese Balkenbush

All Master's Theses

Juniperus is a species-rich and geographically widespread genus of coniferous trees and shrubs. The genus is relatively recently diverged, and has experienced periods of rapid diversification. Recent phylogenetic investigations by others have compared DNA from selected regions of the chloroplast, but the resulting topologies conflict, and some relationships remain unresolved. Their relatively small data sets failed to capture sufficient variation to resolve events of rapid diversification in these closely related taxa. This study provides increased resolution and support by generating a plastome-scale phylogeny for 28 Juniperus species, revealing previously unresolved relationships at both deep and shallow nodes. One-third of the …

Determination Of The Oxidative Capacity Of Soot Toward Gsh And Characterization Of Soot Physicochemical Properties, Kaylee Troth

All Master's Theses

Fine atmospheric particulate matter (PM_2.5) emitted during the combustion of fossil and biomass fuels is known to adversely affect human health. While the underlying mechanisms are thought to be driven by the generation of reactive oxygen species (ROS), specific particle characteristics responsible for this detrimental effect are not well understood. In this research, the quantitative determination of the biologically relevant antioxidant, glutathione (GSH), was optimized for use as an indicator of oxidative stress to shed light on relevant particle characteristics. This was accomplished via fluorescent spectroscopy for GSH determination by way of reaction with o-phthalaldehyde (OPA), a …

Paracest Agents Encapsulated In Reverse Nano-Assembled Capsules (Racs): How Slow Molecular Tumbling Can Quench Cest Contrast, Annah Farashishiko, Jacqueline R. Slack, Mauro Botta, Mark Woods

Chemistry Faculty Publications and Presentations

Although paraCEST is a method with immense scope for generating image contrast in MRI, it suffers from the serious drawback of high detection limits. For a typical discrete paraCEST agent the detection limit is roughly an order of magnitude higher than that of a clinically used relaxation agent. One solution to this problem may be the incorporation of a large payload of paraCEST agents into a single macromolecular agent. Here we report a new synthetic method for accomplishing this goal: incorporating a large payload of the paraCEST agent DyDOTAM3+ into a Reverse Assembled nano-Capsule. An aggregate can be generated between …

Factors Associated With Behavioural Problems Among Children Receiving Anti-Epileptic Drugs, Aswathy K. L Ms, P A Mohammed Kunju Dr, S Mini Dr

Manipal Journal of Nursing and Health Sciences

Children on anti-epileptic drug (AED) therapy have many health problems. Children with epilepsy are at a high risk for poor psychosocial outcomes, even without evident co-morbidities. Apart from the episodic seizures, multiple socio-cultural factors affect their behaviour and health. Objective: Objective of the present study is to find out factors associated with behavioural problems among children receiving anti-epileptic drugs. Methodology: A cross-sectional design was used in the present study. Data were collected from 275 children between 6 to 12 years, who were diagnosed with seizure disorder and on AED for a minimum of six months. Children along with their mothers …

Associated Risk Factors Of Postnatal Depression In South Asian Region - A Narrative Review, Savitha, Linu Sara George, Shyamala G Dr, Judith Angelitta Noronha Dr

Manipal Journal of Nursing and Health Sciences

Background: Postpartum depression (PPD) is a major source of morbidity among women who have recently delivered a child. The recognition and timely identification of risk factors of PPD is very essential to plan appropriate interventions. Objective: Current review aims to explore associated factors of postpartum depression in South Asian region. Methods: Searches of observational cohorts and surveys were performed in Medline (PubMed), CINAHL, ProQuest, and Scopus databases from 2005 to 2016 to identify English language articles. Results: Twenty-four articles were eligible for analysis. The risk factors related to postpartum depression were grouped under following categories: Demographic, psychological, social, obstetric, and …

Childhood Trauma: An Analysis Of Associated Co-Morbidities And Various Psychotherapies, Brittney L. Benson

Physician Assistant Scholarly Project Papers

American and European studies suggest that anywhere from 14 to 67% of children have experienced at least one traumatic event throughout their life. Furthermore, about 13.4% of them go on to develop post-traumatic stress disorder (PTSD) or symptoms (Diehle, J., Opmeer, B.C., Boer, F., Mannarino, A.P., & Lindauer, R.J., 2015). One study suggests that as many as 63.9% of adults suffer from ACEs (Adverse Childhood Experiences) (CDC, 2016). The 4th Edition of the Diagnostic and Statistical Manual (DSM-IV) states that PTSD is the most common Axis I disorder in children that are victims of abuse with statistics ranging from 20% …

Glycemic Control And Type 1 Diabetes Mellitus: Current Standard Treatment Vs. Closed-Loop Insulin Pumps, Kelsey Daley

Physician Assistant Scholarly Project Papers

As of 2015, 9.4% of the US population had a diagnosis of Diabetes Mellitus (DM). Although most of the data sets studied encompass type 1 (T1) and type 2 (T2) DM data in all ages of patients, the focus of this project will be primarily on T1DM patients. A thorough review of the current literature reveals that there are effective methods currently available for the management of T1DM patients. These methods include: closed-loop insulin pumps that integrate a continuous glucose monitor (CGM) and insulin pump into one effective system that calculates the needed insulin doses through complicated algorithms, CGM with …

Decreased Mortality Of Pulmonary Arterial Hypertension In Duchenne Muscular Dystrophy And Down Syndrome, Mitchell Volin

Physician Assistant Scholarly Project Papers

The genetic cause of significant disorders like Down syndrome (DS) and Duchenne muscular dystrophy (DMD) is well documented. Pulmonary arterial hypertension (PAH), a co-morbid condition, is tied to mortality among these groups. If undetected or untreated, in DS, this condition can lead to complications with coronary and lung disease later in life. If undetected or untreated in DMD it can hasten the inevitable prognosis of the disease. Individuals with the ability to communicate their symptoms and actively participate in treatment are at an advantage with subsequent benefit. DS and DMD significantly decrease physical ability and communication capacity.

Genetic research has …

Design And Implementation Of A Secured Iot Device For Prognostics : An Application On Predicting Battery Cycles To Failure, Yuan Liu

Master’s Theses - College of Engineering

"IoT (Internet of Things) connects objects/things through a network to collect data and exchange information between different Internet-enabled objects or systems. All these “things” are basically the embedded system with sensors and communication capability through wired or wireless technologies. Currently, manufacturers are designing smart prod- ucts by using embedded devices powered by software to add new features and smart capa- bilities. By patching more and more software, the products become vulnerable to security attacks. Meanwhile, as mentioned by Intel, the number of Internet connected devices will increase from 15 billion to 200 billion by 2020. The dramatic growth is not …

Design And Analysis Of Nitinol-Embedded Silicone Membranes For Artificial Skin, Paul C. Mazza

Master’s Theses - College of Engineering

"The work on this project has covered many topics. It started off by showing that embedded SMA wires could potentially be used as artificial muscles, specifically by demonstrating the full range of motion of the human eyelid. The embedded wires’ contraction rates could be controlled with an electrical circuit. However, the slow response time of the wire/silicone system was noted, as well as a lack of knowledge of the material properties of the system. For the next phase of this work, a step back was taken to reexamine at the manufacturing method of these samples and to collect data on …

The Effect Of Elaborative Interrogation On The Synthesis Of Ideas From Multiple Sources Of Information, Omer Farooq

Criss Library Faculty Publications

The new Framework for Information Literacy for Higher Education (ACRL, 2016) highlights the ability to synthesize ideas from multiple sources of information as one of the key knowledge practices as students navigate an increasingly complex information landscape. With the introduction of this new document, there is a strong need for evidence-based guidance for information literacy instruction in academic libraries. There is little generalizable empirical research based on cognitive science principles to guide information literacy instruction practice. The present study examined the effectiveness of elaborative interrogation instructional strategy on integration and transformation of ideas from multiple sources of information. 86 participants …

Theoretical Circular Dichroism Spectra Of The Α-Helical Protein Calexitin With The Dipole Interaction Model Including The N-Π* Transition, Akongnwi C. Jungong, Tsvetan Aleksandrov, Rahul Nori, Justin Erickson, Kathryn A. Thomasson, Igor V. Uporov, Ethan R. Austhof, Emily Holt, Felix N. Ngassa

Chemistry Faculty Publications

Circular dichroism (CD) is an important structural biology technique used to study protein dynamics, and most especially the secondary structure of peptides and proteins. Although CD is a technique that is relatively easy to introduce to undergraduate students, the high cost of obtaining a conventional CD instrument and the time required for sample preparation prevents a good number of students from having hands-on experiments demonstrating the principle of CD. Herein, theoretical circular dichroism with the dipole interaction model, DInaMo, is proposed as a tool for introducing students to CD. Using the dipole interaction model, the CD spectra of an α-helical …

The Greek Stoa.” 2014. Entry For The Springer Encyclopedia Of Global Archaeology, Erika Z. Damer

Classical Studies Faculty Publications

Stoa is an ancient Greek term applied to a type of long, narrow, free-standing building with a colonnaded façade. The stoa developed as an architectural form in Archaic Greece, and was most popular from the fifth through first centuries BCE. The stoa should be distinguished from the colonnaded avenues typical of late Hellenistic and Roman cities and from the Roman porticus.

2018-2019 Undergraduate Bulletin, Rochester Institute Of Technology

RIT Digital Archives

No abstract provided.

Case Ip Law, Case Western Reserve University School Of Law, The Spangenberg Center For Law, Technology & The Arts

Case IP Law News

Vol. 5, # 1

• Fusion program examines the fundamentals powering the world’s fourth industrial revolution
• IP Venture Clinic helps launch student startup aimed at increasing efficiency in aerodynamics
• Law school signs international partnership agreement with the IÉSEG School of Management in France
• Graduating 3L Aaron Childs’ paper to be published in the Virginia Sports and Entertainment Law Journal
• Great Lakes Sports & Entertainment Law Academy available online
• Patent Pro Bono Program opens new doors for Ohio inventors
• Professor Craig Nard coauthors new book, Intellectual Property and the Judiciary 
• Zahra Smith joins IP Venture Clinic as director
• Masters in Patent Practice …

Comprehensive Functional And Transcriptional Analysis Of Human Pancreatic Islets From Controls And Individuals With Type 1 Diabetes (T1d), Shristi Shrestha

Dissertations

Type 1 Diabetes is chronic disease where immune moderated destruction of insulin producing β cells disrupts normal glucose homeostasis in the body. Recent findings show that few β cells remain in the pancreatic islets decades after disease onset challenging the previous understanding that β cells are completely destroyed. Overcoming the limited availability of T1D tissues, we studied functional and molecular features of pancreatic islets from individuals with T1D and investigated the nature of the remaining β cells. We found that remnant β cells have normal insulin secretory function and normally express key transcription factor that regulates β cell function. On …

Process Improvement For Maximized Therapeutic Innovation Outcome., Scott A. Waldman, Andre Terzic

Department of Pharmacology and Experimental Therapeutics Faculty Papers

Deconvoluting key biological mechanisms forms the framework for therapeutic discovery. Strategies that enable effective translation of those insights along the development and regulatory path ultimately drive validated clinical application in patients and populations. Accordingly, parity in What vs. How we transform novel mechanistic insights into therapeutic paradigms is essential in achieving success. Aligning molecular discovery with innovations in structures and processes along the discovery-development-regulation-utilization continuum maximizes the return on public and private investments for next-generation solutions in managing health and disease.

Buprenorphine In Neonatal Abstinence Syndrome., Walter K. Kraft

Department of Pharmacology and Experimental Therapeutics Faculty Papers

Infants exposed in utero to opioids will demonstrate a withdrawal syndrome known as neonatal abstinence syndrome (NAS). Buprenorphine is a long-acting opioid with therapeutic use in medication-assisted treatment of opioid dependency in adults and adolescents. Emerging data from clinical trials and treatment cohorts demonstrate the efficacy and safety of sublingual buprenorphine for those infants with NAS who require pharmacologic treatment. Pharmacometric modeling will assist in defining the exposure-response relationships and facilitate dose optimization.

Neonatal Safety Information Reported To The Fda During Drug Development Studies., Debbie Avant, Gerri Baer, Jason N. Moore, Panli Zheng, Alfred Sorbello, Ron Ariagno, Lynne Yao, Gilbert J. Burckart, Jian Wang

Department of Pharmacology and Experimental Therapeutics Faculty Papers

BACKGROUND: Relatively few neonatal drug development studies have been conducted, but an increase is expected with the enactment of the Food and Drug Administration Safety and Innovation Act (FDASIA). Understanding the safety of drugs studied in neonates is complicated by the unique nature of the population and the level of illness. The objective of this study was to examine neonatal safety data submitted to the FDA in studies pursuant to the Best Pharmaceuticals for Children Act (BPCA) and the Pediatric Research Equity Act (PREA) between 1998 and 2015.

METHODS: FDA databases were searched for BPCA and/or PREA studies that enrolled …