Open Access. Powered by Scholars. Published by Universities.®

Digital Commons Network™

Open Access. Powered by Scholars. Published by Universities.®

2018

Discipline
Institution
Keyword
Publication
Publication Type
File Type

Articles 23881 - 23910 of 31369

Full-Text Articles in Entire DC Network

Usft Collection Assessment Dss Annual Report Digital Collections 2017-2018fy, Usf Libraries Jan 2018

Usft Collection Assessment Dss Annual Report Digital Collections 2017-2018fy, Usf Libraries

All Assessment Data

No abstract provided.


Usft Collection Assessment Numeracy 2017-2018fy, Usf Libraries Jan 2018

Usft Collection Assessment Numeracy 2017-2018fy, Usf Libraries

All Assessment Data

No abstract provided.


Usft Services Assessment Dss Annual Report 2017-2018fy, Usf Libraries Jan 2018

Usft Services Assessment Dss Annual Report 2017-2018fy, Usf Libraries

All Assessment Data

No abstract provided.


Organic Carbon Burial In A Freshwater Marsh To Mangrove Transitional Area In Everglades National Park, Kailey R. Comparetto Jan 2018

Organic Carbon Burial In A Freshwater Marsh To Mangrove Transitional Area In Everglades National Park, Kailey R. Comparetto

USF St. Petersburg campus Master's Theses (Graduate)

No abstract provided.


Mos2 Doped Tio2 Nanomaterial And The Enhancement Of Photocatalytic Properties For Solar Disinfection, Timothy Mccolgan Jan 2018

Mos2 Doped Tio2 Nanomaterial And The Enhancement Of Photocatalytic Properties For Solar Disinfection, Timothy Mccolgan

USF St. Petersburg campus Master's Theses (Graduate)

This research project focused on the synthesis, characterization, and environmental application of a photocatalytic nanomaterial composed of molybdenum (IV) disulfide [MOS2] doped titanium dioxide [TiO2]. Extensive research has been conducted on the electronic uses of nanocrystalline MoS2 as a phototransistor and absorptive species (Yin et al., 2012), as well as the photocatalytic effects of TiO2 (Diebold, 2003). Research has also been performed into the generation of oxidizers from TiO2 nanocomposites (Zhang et al., 2015), but as of yet no research has been conducted on a practical application of this material. This project fills that void by evaluating its effectiveness while …


Spatial And Temporal Levels Of Microplastics In Tampa Bay Surface Waters, Laurinda Kinsley Mceachern Jan 2018

Spatial And Temporal Levels Of Microplastics In Tampa Bay Surface Waters, Laurinda Kinsley Mceachern

USF St. Petersburg campus Master's Theses (Graduate)

This study focused on collecting microplastics in Tampa Bay estuarine surface waters in order to identify temporal and spatial differences. While there have been many studies of microplastic contamination in the marine environment over the past decade, quantifying microplastics in urban estuaries is constrained. This study provides an understanding of the abundance, distribution, and composition of microplastics at twenty sites in Tampa Bay as well as four ancillary tributary stations. The objectives of the study were to identify how surrounding land use type, freshwater sources, seasonal changes in precipitation, and wastewater treatment plant discharge affect microplastic concentrations. Water samples were …


Determining Potential Uptake Pathways Of Persistent Organic Pollutants And Heavy Metals In Jobos Bay National Estuarine Research Reserve, Puerto Rico, Ashley Huber Jan 2018

Determining Potential Uptake Pathways Of Persistent Organic Pollutants And Heavy Metals In Jobos Bay National Estuarine Research Reserve, Puerto Rico, Ashley Huber

USF St. Petersburg campus Master's Theses (Graduate)

Fiddler Crab (U. rapax) tissue, mangrove tissue and sediment from crab burrows were collected in Jobos Bay National Estuarine Research Reserve, Puerto Rico, to quantitate Persistent Organic Pollutants (OCs, PCBs, PBDEs) and selected heavy metals (cadmium, barium, mercury, lead, vanadium, chromium, cobalt, nickel, copper, zinc, arsenic and selenium). Once quantified, the concentrations were applied to a bioconcentration factor equation to determine potential uptake pathways of the target analytes. Bioconcentration factors determined that fiddler crabs were the most efficient at concentrating OC pesticides, while mangroves were the most efficient at concentrating PCBs and heavy metals.


Elusive Or Illusive Truth? The Role Of Sensors And Sampling Strategies In Data Collection And Its Implication For Scientific Knowledge And The Pursuit Of Truth, Christopher R. Mchan Jan 2018

Elusive Or Illusive Truth? The Role Of Sensors And Sampling Strategies In Data Collection And Its Implication For Scientific Knowledge And The Pursuit Of Truth, Christopher R. Mchan

USF St. Petersburg campus Master's Theses (Graduate)

No abstract provided.


Soil Accumulation, Accretion, And Organic Carbon Burial Rates In Mangrove Soils Of The Lower Florida Keys: A Temporal And Spatial Analysis, Amanda R. Chappel Jan 2018

Soil Accumulation, Accretion, And Organic Carbon Burial Rates In Mangrove Soils Of The Lower Florida Keys: A Temporal And Spatial Analysis, Amanda R. Chappel

USF St. Petersburg campus Master's Theses (Graduate)

This study compared the temporal variability in rates of soil accumulation, accretion, and organic carbon (OC) burial from three sites in the Lower Florida Keys using two different radiometric dating techniques (210Pb, 14C). Comparison of these rates across various timescales (decadal, centurial, and millennial) permitted examination of temporal responses and drivers. Further, spatial variability was measured for these associated site rates. Differences in geographic location and site morphologies as predicted, were supported by spatial variability in all rates across sites with the exception of centurial rates of accretion. All rates were greater when assessed over shorter (e.g. decadal) timescales. The …


Ciwag - Annual Symposium Report - 2018, The U.S. Naval War College Jan 2018

Ciwag - Annual Symposium Report - 2018, The U.S. Naval War College

CIWAG Events

The Center for Irregular Warfare and Armed Groups at the U.S. Naval War College presented its 10th annual symposium, “Access and Influence,” on June 26-27, 2018. Over 90 U.S. and international academics, practitioners, and operators from the U.S. Navy and other services attended to discuss the strategic and operational challenges associated with irregular warfare and armed groups. Attendees also included representatives from DHS, DIA, FBI, the intelligence community.


Insights Into Key Gene Regulatory Networks In Borrelia Burgdorferi, William Kenneth Arnold Jan 2018

Insights Into Key Gene Regulatory Networks In Borrelia Burgdorferi, William Kenneth Arnold

Theses and Dissertations--Microbiology, Immunology, and Molecular Genetics

Gene regulatory networks are composed of interconnected regulatory nodes created by regulatory factors of multiple types. All organisms finely tune gene expression in order to adapt to and survive within their current niche. Obligate parasitic bacteria are under extreme pressure to quickly and appropriately adapt their gene regulatory programs in order to survive within their given host. Borrelia burgdorferi is one such organism and persists in nature by alternating between two hosts; Ixodes spp. ticks and small vertebrate animals. These two hosts represent drastically different environments; requiring a unique gene regulatory program to survive and transmit between them. Microbiologists have …


The Imprint Of Childhood Abuse On Trauma-Related Shame In Adulthood., Joan Reid Jan 2018

The Imprint Of Childhood Abuse On Trauma-Related Shame In Adulthood., Joan Reid

USF St. Petersburg Campus Faculty Publications

Research has consistently linked residual trauma-related shame among child sexual abuse (CSA) survivors to sexual revictimization, health risk behaviors, and poorer response to mental health treatment. However, questions remain regarding the imprint of childhood maltreatment on trauma-related shame including which CSA characteristics or types of childhood maltreatment contribute to residual shame in adulthood. Using data drawn from a prospective study of 174 primarily African American women with histories of CSA and a matched comparison group, this study explores whether specific characteristics of CSA (familial CSA, CSA with penetration, force used by CSA perpetrator), repeat sexual victimization in adolescence, childhood physical …


Annual Report, Research & Instruction Department, 2017 – 2018, University Of South Florida St. Petersburg. Nelson Poynter Memorial Library Jan 2018

Annual Report, Research & Instruction Department, 2017 – 2018, University Of South Florida St. Petersburg. Nelson Poynter Memorial Library

Library Department and Committee Reports

Annual report for the Research and Instruction Department at the University of South Florida St. Petersburg's Nelson Poynter Memorial Library for the period July 1, 2017 to June 30, 2018.


The Duration Of Untreated Psychosis: A Phenomenological Study, Sarah R. Kamens, Larry Davidson, Emily Hyun, Nev Jones, Jill G. Morawski, Matthew M. Kurtz, Jessica Pollard, Gerrit Ian Van Schalkwyk, Vinrod Srihari Jan 2018

The Duration Of Untreated Psychosis: A Phenomenological Study, Sarah R. Kamens, Larry Davidson, Emily Hyun, Nev Jones, Jill G. Morawski, Matthew M. Kurtz, Jessica Pollard, Gerrit Ian Van Schalkwyk, Vinrod Srihari

Mental Health Law & Policy Faculty Publications

Mounting evidence has indicated that early intervention leads to improved clinical and functional outcomes for young persons experiencing recent onset psychosis. As part of a large early detection campaign, the present study aimed to investigate subjective experiences during the duration of untreated psychosis (DUP), or time between psychosis onset and treatment contact. Participants were 10 young adults participating in early intervention services for psychosis. After DUP was estimated during standardized baseline assessment, participants engaged in qualitative interviews focused on their life experiences prior to treatment and leading up to the present. Mixed methods data analyses compared standardized DUP estimates with …


Unprecedented Diversity Of Ssdna Phages From The Family Microviridae Detected Within The Gut Of A Protochordate Model Organism (Ciona Robusta), Alexandria Creasy, Karyna Rosario, Brittany Leigh, Larry Dishaw, Mya Breitbart Jan 2018

Unprecedented Diversity Of Ssdna Phages From The Family Microviridae Detected Within The Gut Of A Protochordate Model Organism (Ciona Robusta), Alexandria Creasy, Karyna Rosario, Brittany Leigh, Larry Dishaw, Mya Breitbart

Marine Science Faculty Publications

Phages (viruses that infect bacteria) play important roles in the gut ecosystem through infection of bacterial hosts, yet the gut virome remains poorly characterized. Mammalian gut viromes are dominated by double-stranded DNA (dsDNA) phages belonging to the order Caudovirales and single-stranded DNA (ssDNA) phages belonging to the family Microviridae. Since the relative proportion of each of these phage groups appears to correlate with age and health status in humans, it is critical to understand both ssDNA and dsDNA phages in the gut. Building upon prior research describing dsDNA viruses in the gut of Ciona robusta, a marine invertebrate model system …


Genome Sequence Of Pm2-Like Phage Cr39582, Induced From A Pseudoalteromonas Sp. Isolated From The Gut Of Ciona Robusta, Brittany Leigh, Mya Breitbart, Hanna Oksanen, Dennis Bamford, Larry Dishaw Jan 2018

Genome Sequence Of Pm2-Like Phage Cr39582, Induced From A Pseudoalteromonas Sp. Isolated From The Gut Of Ciona Robusta, Brittany Leigh, Mya Breitbart, Hanna Oksanen, Dennis Bamford, Larry Dishaw

Marine Science Faculty Publications

Phage Cr39582 was induced by mitomycin C from Pseudoalteromonas sp. strain Cr6751, isolated from a marine invertebrate gut. Pseudoalteromonas phage Cr39582 has 85% pairwise nucleotide identity with phage PM2 but lacks sequence homology in the spike protein. This report supports previous bioinformatic identification of corticoviral sequences within aquatic bacterial genomes.


Elementary Student Outreach Activity Demonstrating The Use Of Phage Therapy Heroes To Combat Bacterial Infections, Mya Breitbart, Kema Malki, Natalie Sawaya, Chelsea Bonnain, Mark Martin Jan 2018

Elementary Student Outreach Activity Demonstrating The Use Of Phage Therapy Heroes To Combat Bacterial Infections, Mya Breitbart, Kema Malki, Natalie Sawaya, Chelsea Bonnain, Mark Martin

Marine Science Faculty Publications

No abstract provided.


Discovery Of Four Novel Circular Single-Stranded Dna Viruses In Fungus-Farming Termites, Mason Kerr, Karyna Rosario, Christopher C. M. Baker, Mya Breitbart Jan 2018

Discovery Of Four Novel Circular Single-Stranded Dna Viruses In Fungus-Farming Termites, Mason Kerr, Karyna Rosario, Christopher C. M. Baker, Mya Breitbart

Marine Science Faculty Publications

Here, we describe four novel circular single-stranded DNA viruses discovered in fungus-farming termites (Odontotermes sp.). The viruses, named termite-associated circular virus 1 (TaCV-1) through TaCV-4, are most similar to members of the family Genomoviridae and were widely detected in African termite mounds.


Evaluation Of Marine Zooplankton Community Structure Through Environmental Dna Metabarcoding, Anni Djurhuus, Kathleen Pitz, Natalie Sawaya, Jaimie Rojas‐Márquez, Brianna Michaud, Enrique Montes, Frank E. Muller-Karger, Mya Breitbart Jan 2018

Evaluation Of Marine Zooplankton Community Structure Through Environmental Dna Metabarcoding, Anni Djurhuus, Kathleen Pitz, Natalie Sawaya, Jaimie Rojas‐Márquez, Brianna Michaud, Enrique Montes, Frank E. Muller-Karger, Mya Breitbart

Marine Science Faculty Publications

Zooplankton dominate the abundance and biomass of multicellular animals in pelagic marine environments; however, traditional methods to characterize zooplankton communities are invasive and laborious. This study compares zooplankton taxonomic composition revealed through metabarcoding of the cytochrome oxidase I (COI) and 18S rRNA genes to traditional morphological identification by microscopy. Triplicates of three different sample types were collected from three coral reef sites in the Florida Keys National Marine Sanctuary: (1) 1 L surface seawater samples prefiltered through 3 μm filters and subsequently collected on 0.22 μm filters for eDNA (PF‐eDNA); (2) 1 L surface seawater samples filtered on …


Linking Capacity Development To Goos Monitoring Networks To Achieve Sustained Ocean Observation, Frank E. Muller-Karger Jan 2018

Linking Capacity Development To Goos Monitoring Networks To Achieve Sustained Ocean Observation, Frank E. Muller-Karger

Marine Science Faculty Publications

Developing enduring capacity to monitor ocean life requires investing in people and their institutions to build infrastructure, ownership, and long-term support networks. International initiatives can enhance access to scientific data, tools and methodologies, and develop local expertise to use them, but without ongoing engagement may fail to have lasting benefit. Linking capacity development and technology transfer to sustained ocean monitoring is a win-win proposition. Trained local experts will benefit from joining global communities of experts who are building the comprehensive Global Ocean Observing System (GOOS). This two-way exchange will benefit scientists and policy makers in developing and developed countries. The …


Seasonal Patterns In Phytoplankton Biomass Across The Northern And Deep Gulf Of Mexico: A Numerical Model Study, Fabian A. Gomez, Sang-Ki Lee, Frank J. Hernandez Jr, Frank E. Muller-Karger, John T. Lamkin Jan 2018

Seasonal Patterns In Phytoplankton Biomass Across The Northern And Deep Gulf Of Mexico: A Numerical Model Study, Fabian A. Gomez, Sang-Ki Lee, Frank J. Hernandez Jr, Frank E. Muller-Karger, John T. Lamkin

Marine Science Faculty Publications

Biogeochemical models that simulate realistic lower-trophic-level dynamics, including the representation of main phytoplankton and zooplankton functional groups, are valuable tools for improving our understanding of natural and anthropogenic disturbances in marine ecosystems. Previous three-dimensional biogeochemical modeling studies in the northern and deep Gulf of Mexico (GoM) have used only one phytoplankton and one zooplankton type. To advance our modeling capability of the GoM ecosystem and to investigate the dominant spatial and seasonal patterns of phytoplankton biomass, we configured a 13-component biogeochemical model that explicitly represents nanophytoplankton, diatoms, micro-, and mesozooplankton. Our model outputs compare reasonably well with observed patterns in …


Impacts Of 40 Years Of Land Cover Change On Water Quality In Tampa Bay, Florida, Matthew J. Mccarthy, Frank E. Muller-Karger, Daniel Otis, Pablo Méndez-Lázaro Jan 2018

Impacts Of 40 Years Of Land Cover Change On Water Quality In Tampa Bay, Florida, Matthew J. Mccarthy, Frank E. Muller-Karger, Daniel Otis, Pablo Méndez-Lázaro

Marine Science Faculty Publications

Land cover changes in the Tampa Bay watershed (Florida) over the past four decades were examined along with precipitation and wind observations to help understand causes of long-term changes in turbidity and chlorophyll concentration within the Tampa Bay estuary. Water quality showed a statistically significant relationship to land cover fraction in the watershed compared to long-term precipitation or wind stress. Redundancy Analyses with Akaike’s Information Criterion and non-parametric multiple regressions determined that turbidity and chlorophyll concentration decreased bay-wide from 1974–2012 with increased developed land fraction (R2 > 0.75, p-value < 0.05). Various segments of the estuary showed different significant responses to developed land (R2 > 0.75, p-value < 0.05), agricultural land (R2 > 0.93, p-value < 0.02), bare land (R2 = 0.77, p-value = 0.001), and wind stress (R2 = 0.91, p-value = 0.04) at different times of year.


Advancing Marine Biological Observations And Data Requirements Of The Complementary Essential Ocean Variables (Eovs) And Essential Biodiversity Variables (Ebvs) Frameworks, Frank E. Muller-Karger, Enrique Montes Jan 2018

Advancing Marine Biological Observations And Data Requirements Of The Complementary Essential Ocean Variables (Eovs) And Essential Biodiversity Variables (Ebvs) Frameworks, Frank E. Muller-Karger, Enrique Montes

Marine Science Faculty Publications

Measurements of the status and trends of key indicators for the ocean and marine life are required to inform policy and management in the context of growing human uses of marine resources, coastal development, and climate change. Two synergistic efforts identify specific priority variables for monitoring: Essential Ocean Variables (EOVs) through the Global Ocean Observing System (GOOS), and Essential Biodiversity Variables (EBVs) from the Group on Earth Observations Biodiversity Observation Network (GEO BON) (see Data Sheet 1 in Supplementary Materials for a glossary of acronyms). Both systems support reporting against internationally agreed conventions and treaties. GOOS, established under the auspices …


Understanding The Interactability Of Chikungunya Virus Proteins Via Molecular Recognition Feature Analysis, Ankur Singh, Ankur Kumar, Vladimir N. Uversky, Rajanish Giri Jan 2018

Understanding The Interactability Of Chikungunya Virus Proteins Via Molecular Recognition Feature Analysis, Ankur Singh, Ankur Kumar, Vladimir N. Uversky, Rajanish Giri

Molecular Medicine Faculty Publications

The chikungunya virus (CHIKV) is an alphavirus that has an enveloped icosahedral capsid and is transmitted by Aedes sp. mosquitos. It contains four non-structural proteins, namely nsP1, nsP2, nsP3, and nsP4, encoded at the 5′ end of the genome, and five structural proteins encoded at the 3′ end of the genome, including three glycosylated proteins, namely E1, E2, E3, a small 64 amino-acids glycoprotein 6K, and one non-glycosylated nucleocapsid protein C. The surface of this positive-stranded RNA alphavirus is covered with 80 trimeric glycoprotein spikes, which facilitate viral access into the host cell, with each consisting of three copies of …


Characterization Of The Virulence Of A Non-Rt027, Non-Rt078 And Binary Toxin-Positive Clostridium Difficile Strain Associated With Severe Diarhea, Chunhui Li, Céline Harmanus, Duolong Zhu, Xiujuan Meng, Shaohui Wang, Juping Duan, Sidi Liu, Chenchao Fu, Pengcheng Zhou, Ruisi Liu, Anhua Wu, Ed J. Kuijper, Wiep Klaas Smits, Lei Fu, Xingmin Sun Jan 2018

Characterization Of The Virulence Of A Non-Rt027, Non-Rt078 And Binary Toxin-Positive Clostridium Difficile Strain Associated With Severe Diarhea, Chunhui Li, Céline Harmanus, Duolong Zhu, Xiujuan Meng, Shaohui Wang, Juping Duan, Sidi Liu, Chenchao Fu, Pengcheng Zhou, Ruisi Liu, Anhua Wu, Ed J. Kuijper, Wiep Klaas Smits, Lei Fu, Xingmin Sun

Molecular Medicine Faculty Publications

The expression of the Clostridium difficile binary toxin CDT is generally observed in the RT027 (ST1) and RT078 (ST11) C. difficile isolates, which are associated with severe C. difficile infection (CDI). However, we recently reported that the non-RT027 and non-RT078 C. difficile strain LC693 (TcdA+TcdB+ CDT+, ST201) caused severe diarrhea in a patient in Xiangya Hospital in China. C.difficile LC693 is a member of Clade 3, and in this study, we identified LC693 as RT871 and compared its virulence and pathogenicity to those of C.difficile R20291 (TcdA+TcdB+CDT+, ST1/RT027), UK6 (TcdA+TcdB+CDT+, ST35/RT027), CD630 (TcdA+TcdB+CDT−, ST54, RT012), and 1379 (TcdA+TcdB+CDT−, ST54/RT012), with …


Elucidating The Inhibitory Potential Of Designed Peptides Against Amyloid Fibrillation And Amyloid Associated Cytotoxicity, Mohammad K. Siddiqi, Parvez Alam, Tabish Iqbal, Nabeela Majid, Sadia Malik, Saima Nusrat, Aftab Alam, Mohd R. Ajmal, Vladimir N. Uversky, Rizwan H. Khan Jan 2018

Elucidating The Inhibitory Potential Of Designed Peptides Against Amyloid Fibrillation And Amyloid Associated Cytotoxicity, Mohammad K. Siddiqi, Parvez Alam, Tabish Iqbal, Nabeela Majid, Sadia Malik, Saima Nusrat, Aftab Alam, Mohd R. Ajmal, Vladimir N. Uversky, Rizwan H. Khan

Molecular Medicine Faculty Publications

Inhibition of fibrillation process and disaggregation of mature fibrils using small peptide are the promising remedial strategies to combat neurodegenerative diseases. However, designing peptide-based drugs to target β-sheet-rich amyloid has been a major challenge. The current work describes, for the first time, the amyloid inhibitory potential of the two short peptides (selected on the basis of predisposition of their amino acid residues toward β-sheet formation) using combination of biophysical, imaging methods, and docking approaches. Results showed that peptides employed different mechanisms to inhibit the amyloid fibrillation. Furthermore, they were also effective in blocking the amyloid fibrillation pathway. In contrary to …


Anti-Correlation Between The Dynamics Of The Active Site Loop And C-Terminal Tail In Relation To The Homodimer Asymmetry Of The Mouse Erythroid 5-Aminolevulinate Synthase, Insung Na, Dominique Catena, Min J. Kong, Gloria C. Ferreira, Vladimir N. Uversky Jan 2018

Anti-Correlation Between The Dynamics Of The Active Site Loop And C-Terminal Tail In Relation To The Homodimer Asymmetry Of The Mouse Erythroid 5-Aminolevulinate Synthase, Insung Na, Dominique Catena, Min J. Kong, Gloria C. Ferreira, Vladimir N. Uversky

Molecular Medicine Faculty Publications

Biosynthesis of heme represents a complex process that involves multiple stages controlled by different enzymes. The first of these proteins is a pyridoxal 5′-phosphate (PLP)-dependent homodimeric enzyme, 5-aminolevulinate synthase (ALAS), that catalyzes the rate-limiting step in heme biosynthesis, the condensation of glycine with succinyl-CoA. Genetic mutations in human erythroid-specific ALAS (ALAS2) are associated with two inherited blood disorders, X-linked sideroblastic anemia (XLSA) and X-linked protoporphyria (XLPP). XLSA is caused by diminished ALAS2 activity leading to decreased ALA and heme syntheses and ultimately ineffective erythropoiesis, whereas XLPP results from “gain-of-function” ALAS2 mutations and consequent overproduction of protoporphyrin IX and increase in …


Recombinant Fusion Protein Joining E Protein Domain Iii Of Tick-Borne Encephalitis Virus And Hsp70 Of Yersinia Pseudotuberculosis As An Antigen For The Ti-Complexes, Vasily Golotin, Nina M. Sanina, Ludmila Davydova, Natalia Chopenko, Andrey Mazeika, Manuel Roig, Valery L. Shnyrov, Vladimir N. Uversky, Eduard Kostetsky Jan 2018

Recombinant Fusion Protein Joining E Protein Domain Iii Of Tick-Borne Encephalitis Virus And Hsp70 Of Yersinia Pseudotuberculosis As An Antigen For The Ti-Complexes, Vasily Golotin, Nina M. Sanina, Ludmila Davydova, Natalia Chopenko, Andrey Mazeika, Manuel Roig, Valery L. Shnyrov, Vladimir N. Uversky, Eduard Kostetsky

Molecular Medicine Faculty Publications

Domain III (DIII) of the tick-borne encephalitis virus (TBEV) protein E contains epitopes, which induce antibodies capable of neutralizing the virus. To enhance the immunogenicity of this protein, which has a low molecular weight, the aim of the present work was to express, isolate, and characterize a chimeric protein based on the fusion of the bacterial chaperone HSP70 of Yersinia pseudotuberculosis and EIII (DIII + stem) as a prospective antigen for an adjuvanted delivery system, the tubular immunostimulating complex (TI-complex). The chimeric construction was obtained using pET-40b(+) vector by ligating the respective genes. The resulting plasmid was transformed into DE3 …


Intrinsically Disordered Proteins And The Janus Challenge, Prakash Kulkarni, Vladimir N. Uversky Jan 2018

Intrinsically Disordered Proteins And The Janus Challenge, Prakash Kulkarni, Vladimir N. Uversky

Molecular Medicine Faculty Publications

To gain a new insight into the role of proteins in the origin of life on Earth, we present the Janus Challenge: identify an intrinsically disordered protein (IDP), naturally occurring or synthetic, that has catalytic activity. For example, such a catalytic IDP may perform condensation reactions to catalyze a peptide bond or a phosphodiester bond formation utilizing natural/un-natural amino acids or nucleotides, respectively. The IDP may also have autocatalytic, de novo synthesis, or self-replicative activity. Meeting this challenge may not only shed new light and provide an alternative to the RNA world hypothesis, but it may also serve as an …


Ecosystem Model Of The Entire Beaufort Sea Marine Ecosystem: A Temporal Tool For Assessing Food-Web Structure And Marine Animal Populations From 1970 To 2014, Paul M. Suprenand, Cameron H. Ainsworth, Carie Hoover Jan 2018

Ecosystem Model Of The Entire Beaufort Sea Marine Ecosystem: A Temporal Tool For Assessing Food-Web Structure And Marine Animal Populations From 1970 To 2014, Paul M. Suprenand, Cameron H. Ainsworth, Carie Hoover

Marine Science Faculty Publications

The Beaufort Sea coastal-marine ecosystem is a 476,000 km2 area in the Arctic Ocean, which extends from -112.5 to -158.0° longitude to 67.5 to 75.0° latitude. Within this polar area the United States indigenous communities of Barrow, Kaktovik, and Nuiqsut, and the Canadian indigenous communities of Aklavik, Inuvik, Tuktoyaktuk, Paulatuk, Ulukhaktok, and Sachs Harbour, subsist by harvesting marine mammals, fish, and invertebrates to provide the majority of their community foods. The Beaufort Sea coastal-marine ecosystem includes many specialized marine animals whose life history is tied to the sea ice, such as polar bears that rely on sea-ice for foraging activities …